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Peptide bond formation is catalyzed by peptidyl transferase. The carboxyl end of the amino acid bound to the tRNA in this reaction and in the P site is uncoupled from the tRNA and becomes joined by a peptide bond to the amino set of the amino acid connect to the tRNA in the A site. A protein with peptidyl transferase activity has certainly not been isolated. The reason is now obvious; in E. coli at least, the peptidyl transfers activity is related with part of the 23S rRNA in the vast ribosomal subunit. Alternatively, peptidyl transferase is a ribozyme, a catalytic activity which resides in an RNA molecule.
How to Calculate the Biological Value of Protein? A method for determining the biological value of proteins was developed by Mitchell in 1925. It measures the quantity of dieta
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