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Time-of-entry experiments indicate that a gene for resistance to kanamycin, kan-r, is closely linked to a gene for resistance to bacteriophage T5, T5-r. A geneticist has strains of two genotypes available, kan-r T5-s (resistant to kanamycin, sensitive to phage T5) and kan-s T5-r (sensitive to kanamycin, resistant to phage T5). In an experiment using phage P1 to learn whether the genes are close enough for cotransduction, how would the experiment be done:
A) Which strain would you infect with the P1 to obtain the transducing phage?
B) Which strain would you infect with the transducing phage?
C) Which marker would you select to obtain transductants?
D) How would you determine whether the genes were cotransduced?
Q. Illustrate phase diagram? Ans. There are two things which influence phase change: 1. The pressure exerted on the surface of a substance. 2. The addition or rele
merits& demerits of free radical mechanism
Q. What is standard electrode potentials of lanthanides? The standard electrode potentials of lanthanides for the half reaction Ln 3+ (aq) + 3e ----------------> Ln (s)
Explain Bromination of N-N-dimethylaniline Bromination of N,N-dimethylaniline is faster because nitrogen has an unshared electron pair that can stabilize the carbocation inter
In lab, you're having fun along with buffers based onto a 0.1 M solution of sodium phosphate at pH7.0. Into this system, there are three of pK A s as: 12.7, 7.21 and 2.12.
Silver salt method for acids - Chemical methods It is based upon the fact that silver salt of an organic acid on heating provides residue of metallic silver.
Tert-butylamine Tert-butylamine cannot be prepared by the Gabriel synthesis because it would require that the nitrogen of phthalimide be alkylated with tert-butyl bromide in an
Uses of Phenol - Hydrocarbon Phenol is extensively used in industry. The important applications of phenol are (i) Phenol is used as an antiseptic in soaps, lotions and ointm
Q. Show Redox Reactions as Electron Transfer? Ans. Oxidation is a process where electrons are lost, and reduction is a process where electrons are gained. For example,
When 100 ml of M/10 H2SO4 is mixed with 500 ml of My/10 NaOH then nature of resulting solution and normality of excess reactant left is
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