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Nucleic acid thermodynamics is the learning of how temperature affects the nucleic acid structure of dsDNA (double-stranded DNA). The melting temperature (Tm) is explained as the temperature at that half of the DNA strands are in the double-helical state and half are in the random coil state. The Tm is depends on the length of the DNA molecule and its particular nucleotide sequence. The DNA, when in a state where its two strands are dissociated example for the dsDNA molecule be as two independent strands is given to as having been denatured by the high temperature
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