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Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.
What are the synthetic auxins and what are their uses? The Synthetic auxins like naphthalenic acid (NAA) and indolebutyric acid (IBA) are substances similar to IAA (a natural a
Procedure for Morphological Characteristics of Yeast Cells? Now carry out the exercise following the steps enumerated herewith: (1) Label the clean, non-greasy slide. Put fe
Psychological Problems Failure to fulfill the patient's expectations and failure to gain the patient's acceptance and satisfaction constitutes a psychological failure. It is hi
Demographic Transition As we saw earlier, death rates and birth rates have been nearly equal throughout much of human history. However, in Western Europe, death rates began to
M a s t iti s Mastitis is one the important diseases in cows that has serious economic impact on livestock production. The inflammation of mammary gland is associated with
MITOCHONDRIA It is the power house of the cell because they are the major centers of release of energy in the aerobic respiration. Mitochondria and chloroplast both are a
WHAT IS SYMBIOTIC THEORY. PLEASE EXPLAIN THIS TO ME IN A VERY SIMPLE WAY.
excretory organs in spiders?
Define Advantages of using Yeast as a source of Protein? 1. Large size, hence separation from the culture medium is easy. 2. As the pH of the growth is towards acidic side,
R e striction enzyme analysis: The restriction endonuclease analysis of viral DNA fragments or DNA fingerprinting discovered in 1970s has revolutionized the study o
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