Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

Hospitalization, Hospitalization Hospitalization is stressful experien...

Hospitalization Hospitalization is stressful experience  for children,  adolescents  and their families.  It is an interruption  of the child's active cycle of growth and deve

Before purchasing - nursing software, Before Purchasing  Ideally it...

Before Purchasing  Ideally it's wise to buy a readymade software with options to further customize it to suit your managerial functions.   A totally Custom-made softwar

Which are specialized conductive tissues of the plants, Which are specializ...

Which are specialized conductive tissues of the plants? Vascular tissues of the plants are the phloem and the xylem. The Xylem is the plant tissue that forms the vessels that c

Explain saturated fatty acids, Explain saturated fatty acids Some of th...

Explain saturated fatty acids Some of the more significant saturated fatty acids with their systematic and common names are listed in table, and some of the unsaturated fatty a

Counselling diabetic travellers, Counselling Diabetic Travellers The c...

Counselling Diabetic Travellers The counsellor needs to educate a diabetic patient and /or his family members to following issues when such patients plan to travel: ·  Plan

Explain about anabolism, Q. Explain about Anabolism? Anabolism is a pro...

Q. Explain about Anabolism? Anabolism is a process of synthesis or making of larger or complex molecules from smaller molecules. The molecules are of different kinds like hormo

Define steps in the development of exchange list, Define Steps in the Devel...

Define Steps in the Development of Exchange List? As mentioned above, when we group together similar food items so that each supplies a constant amount of a particular nutrient

Explain the buccal perforations, Buccal Perforations Buccal concavitie...

Buccal Perforations Buccal concavities in the bone can result in some threads of the implant being exposed. Where these are very circumscribed and covered with thick and well-

What is oxidative phosphorylation, What is oxidative phosphorylation? O...

What is oxidative phosphorylation? Oxidative phosphorylation is  the process by which ADP is phosphorylated by Pi  to ATP  in  the  respiratory chain.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd