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Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.
Explain about the Physiological Accommodation Physiological Accommodation contractile power of the ciliary muscle required to change the focussing power of the eye by ID. Meas
how are excretory products brought to the excretory organs to be excreted?
Types of Variations - Somatogenic and Blastogenic Variation Changes in somatic parts of the body are somatic variations e.g. wrestler's muscle, circumcised penis of mus
Q. Proteins requirement in diabetes? Proteins: Proteins should be provided in adequate amounts to maintain a normal body composition and prevent depletion of lean tissue mass.
First Stage of Invagination The large endodermal cells that are remaining in the vegetal plate move laterally towards the centre of the plate and fill the gap in the vegetal
Q What are the kinds of cell respiration? There are two types of cell respiration aerobic cell respiration a reaction with participation of molecular oxygen (O2) and anaerobic
Explain the Occurrence of vitamin B 6 Vitamin B 6 activity is attributed to the 3 compounds-pyridoxol (pyridoxine), pyridoxal and pyridoxamine, generally comprised in the vit
Hom o sapiens sapiens (MODER N MAN) - Developed from Cro-Magnon about 10,000 years ago after last glacial period in the regions of Caspian and Mediterranean seas.
Consider cell with a diploid chromosome number of 4 (2n=4), one pair of the chromosomes is metacentric,the other is acrocentric. The metacentric is labeled a 1 a 1 while the acr
Name the two groups of nephrons on the basis of their position in the kidney. How are they different from each other? a) How is the halophyte Rhizophora adapted to survive in i
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