Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

Determine the two different alleles, What is meant by saying that in relati...

What is meant by saying that in relation to a given trait conditioned by a gene with two different alleles the gametes are always "pure"? To say that gametes are pure means tha

How are the male gametophytes formed in angiosperms, Q. How are the male ga...

Q. How are the male gametes and the male gametophytes formed in angiosperms? In the anthers of every stamen there are pollen sacs. Inside the pollen sacs there are microspore m

Importance of compliance, It is important to explain to the patient about i...

It is important to explain to the patient about importance of taking treatment regularly. In case he misses the dose or takes medicine or insulin irregularly, explain the side effe

Define about the ascorbic acid (vitamin c), Define about the Ascorbic acid ...

Define about the Ascorbic acid (vitamin C)? Ascorbic acid was discovered as the anti-scurvy vitamin. Vitamin C (chemical names ascorbic acid and ascorbate) is the six-carbon la

Explain bacterial vaginosis, Bacterial vaginosis  The role of sexual tr...

Bacterial vaginosis  The role of sexual transmission is unclear in bacterial vaginosis, in which normal H2O2-producing Lactobacillus  species are replaced by overgrowth with  G

Drug dependence, DRUG DEPENDENCE - Some drugs are prescribed by doctor ...

DRUG DEPENDENCE - Some drugs are prescribed by doctor for the prevention or treatment of a disease. Some drugs are used for long periods. A person becomes addict to these

Historic example of evolutionary quantitative geneticists, Define Historic ...

Define Historic Example of Evolutionary quantitative geneticists? Evolutionary quantitative geneticists have developed tools to create predictions about the short-range evoluti

Explain the histologic techniques, Explain the histologic techniques A...

Explain the histologic techniques A clinician has to base the diagnosis only on the clinical and radiographic findings as microbiologic and histologic techniques are restricte

Cadmium, C adm iu m Like lead and mercury, cadmium is also non-essen...

C adm iu m Like lead and mercury, cadmium is also non-essential biologically. The industrial use of cadmium in plastic production, electroplating and manufacture of alloys a

Expalain briefly vitamin A, Vitamin A (Retinol) Purified vitamin A is a...

Vitamin A (Retinol) Purified vitamin A is a viscous yellowish oil. It is freely soluble in most organic solvents such as methanol, ether, acetone, chloroform and petroleum ethe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd