Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

Secondary production - ecosystem, Secondary Production - Ecosystem Th...

Secondary Production - Ecosystem The net primary production is the only energy available to consumers or heterotrophs including man. Herbivores such as cow or deer gaze upon

What nerve is being compressed, what major nerve do you think is being comp...

what major nerve do you think is being compressed when a person often feel pain in the posterior surface of the thigh radiating to the area behind the knee and where is the likely

Germ layers - metazoa, Germ Layers - Metazoa The infolding of the blas...

Germ Layers - Metazoa The infolding of the blastoderm of the blastula forms a gastrula with two or more layers. The outer layer is the ectoderm' and the endoderm lines the inn

Working of eye, WORKIN G OF EYE - Human eye is sensitive to light wave...

WORKIN G OF EYE - Human eye is sensitive to light wavelenght ranging from 380 - 760 nm. Bees, ants, spiders and gold fish can see uttraviolet light which is invisible to hu

Explain cumulative interceptive supportive therapy, Cumulative Interceptive...

Cumulative Interceptive Supportive Therapy (ITI Consensus report) Systematic and continuous monitoring of the periimplant tissue conditions is recommended for the diagnosis of

Why ups is so important to health practitioners, Give a short explanation o...

Give a short explanation of what UPS really means and why it is so important to health practitioners and consumers/patients.

Sensor measures the current value of the controlled variable, In a properly...

In a properly functioning negative feedback system A. value of the controlled variable will always be very close to the threshold value when the system is in steady state.

Calorie value, THE CALORIE VALUE - The amount of heat liberated fr...

THE CALORIE VALUE - The amount of heat liberated from complete combusion of 1gm food in a bomb caloriemeter (a closed metal chamber filled with O ) is its gross calorific

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd