Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

Signify the difference between bryophytes and tracheophytes, Which of the b...

Which of the below determines the major difference between bryophytes (pron: bry-oh-fites) and tracheophytes (pron: tray-key-o-fites)? a) Tracheophytes can make their own food

What are the four groups of protozoans, What are the four groups of protozo...

What are the four groups of protozoans? The four major groups of protozoans are the sarcodines (that form pseudopods, like amoebae), the mastigophores (flagellated, such as the

If the titration is done by adding naoh to a solution, If the titration is ...

If the titration is done by adding NaOH to a solution containing 2.661 moles of lysine, how many milliliters (mL) of a 5 M solution of NaOH would you have to add to reach point "E"

Hormones secreted by thyroid gland, Hormone s . The thyroid gland secretes...

Hormone s . The thyroid gland secretes following hormones. Thyroxine (tetraiodothyronine or T 4 )' and tri-iodothyronine or T 3 are secreted by the thyroid follicular cells.

Nutrition, prove oxygen is produced during photosynthesis in the presence o...

prove oxygen is produced during photosynthesis in the presence of light

Heteromorphic types - intra specific incompatibility, Heteromorphic Types -...

Heteromorphic Types - Intra specific Incompatibility Plants of the same species produce flowers that differ in morphology. This involves two (distyly) or three (tristyly) morp

Louping ill, Louping ill This is an infectious encephalomyelitis of sh...

Louping ill This is an infectious encephalomyelitis of sheep and man occurring during spring and summer when the vectors like ticks are active. It is reported from British Isl

Air pollutants - effects on plants, Air pollutants - Effects on plants ...

Air pollutants - Effects on plants Particulate matter such as cement, coal, petro coke, dust, and fly-ash screen the light and thus change its quantity and quality. Dust plug

Animal nutrition - zoology, Anima l Nutrition Nutrition is the procu...

Anima l Nutrition Nutrition is the procurement of substances necessary for growth, maintenance and activities of a living body. The substances used for nutrition are called

How can the fermentation explain the origin of muscle cramps, How can the k...

How can the knowledge about fermentation explain the origin of muscle cramps and pains after intense physical exertion? A typical fermentation process because of oxygen scarcit

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd