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Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.
What is Central Shunt in Palliative Operations? When the pulmonary artery branches are small, an interposition graft could be used as a shunt between main pulmonary artery and
Vitamin B 2 (Riboflavin) Riboflavin is a yellow to orange-yellow, crystalline powder of faint odour and intensely bitter taste. Its solubility in water is very slight and depe
Osmoregulation in Animals Osmoregulation associates to the regulation of water and ionic content of the body of non-chordate metazoans. These might be osmoconformers / osmoreg
PULSE Pulse frequency and heart rate are identical i.e. 72. It can be felt in superficial arteries as in wrist (radial artery [thumb] and ulner artery of brachial art.),
Differentiate between Total Fertility Rate (TFR) and Replacement Level (RL) Due to uncontrolled excessive hunting the population of tigers in a forest becomes Zero. Discuss the
Describe in brief about failing implant The failing implant may show evidence of pocketing, bleeding upon probing, purulence, and indicates the bone loss patterns are progressi
S T ARC H - Polymer of a-D-glucose. Starch is glucosan homopolysaccharide which is the major reserve food of plants. Starch is formed as an end product of photosynt
Q. What are the main types of cancer that affect humans? An Excluding skin cancer, that are the more easily detected and so cases are registered in larger number, the main type
define alimentary canal
What is Barfoed's test and its principle? This test is a specific test for monosaccharides. Principle This test is also a copper reduction test but differs from Fehling
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