Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

What are differentiations of the cell membrane, In some types of cells, the...

In some types of cells, the cell membrane present differentiations that are essential for the specific functions of the cells. The major differentiations are the microvilli and the

Explain nelfinavir, Explain Nelfinavir (NFV, Viracept)  Nelfinavir is a...

Explain Nelfinavir (NFV, Viracept)  Nelfinavir is a commonly used protease inhibitor because it is well tolerated. It appears to be less potent than lopinavir/ritonavir or efav

Signify the term - analogy, Signify the term - Analogy, Analogous? Refe...

Signify the term - Analogy, Analogous? Refers to structures that do not have the same evolutionary origin but have the same functions. The wings of a bat and insect are analogo

Define sugar as a type of carbohydrates, Define Sugar as a type of Carbohyd...

Define Sugar as a type of Carbohydrates? This group includes monosaccharide and disaccharides. List down the names of sugars under both these groups and check it out with those

What are the two major morphological patterns of cnidarians, Q. What are th...

Q. What are the two major morphological patterns of cnidarians? Concerning locomotion how do these forms differentiate from each other? Morphologically, cnidarians classify as

In vitro studies - nutrition of embryo, In vitro Studies - Nutrition of Emb...

In vitro Studies - Nutrition of Embryo Studies involving culture of embryos excised of various stages of development (i.e., early globular proembryo, late globular proembryo o

Features of gateway vector, Could any of the following features be useful i...

Could any of the following features be useful in a gateway vector; (i) Origin of replication (ii) multiple cloning site (iii) blue/white selection (iv) M13 forward and rev

Explain iodophors, In these disinfectants, iodine is incorporated along wi...

In these disinfectants, iodine is incorporated along with a detergent. They are not as effective as hypochlorites against bacterial spore but also get inactivated in the pres

Polypeptide chains of hemoglobin, Q. In sickle cell anemia, a hereditary di...

Q. In sickle cell anemia, a hereditary disease, there is replacement of one amino acid by another in one of the four polypeptide chains of hemoglobin. In this case are all of the s

How campylobacteriosis occurs in humans, How CAMPYLOBACTERIOSIS  occurs in...

How CAMPYLOBACTERIOSIS  occurs in humans C. jejuni frequently contaminates raw chicken. Survey shows that 20-100% of retail chickens are contaminated. Many healthy chickens car

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd