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Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.
Which term is used to describe organisms that live on or in the bottom of an ocean or lake. Such organisms can be found anywhere from the shoreline to greatest ocean depths? a)
Osteomalacia Similar to rickets in young growing animals, osteomalacia is a disease of mature animals caused by absolute or relative deficiency of any one or combination of cal
digram or mammalian testis
DIFFERENCE S BETWEEN MYOSIN AND ACTIN FILAMENTS Myosin filaments (primary filaments) Actinfilament s (secondary filaments)
Progressive senescence - Senescence This is the third category of senescence. Here also the leaves are shed but it is gradual senescence of leaves up the stem. For example, th
Why Water is a essential nutrient for life? In this unit, we learnt that water in spite of being ignored, is an essential nutrient required for life. Though the content of tota
What are the scopes of Zoology?..
What are the indications of Cardiac Transplantation ? Indication : Patients in NYHA class IV with heart failure, refractory to medical management caused by end stage corona
In general compare and contrast the three functional classifications of joints according to movement. What are two characteristics that make synovial joints unique and different fr
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