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Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.
Explain Diagnostic Approach to Congenital Heart Disease ? It is often easy to recognise the presence of CHD in older children and adults. As in any other form of heart disease
he recombinant plasmid extracted from the surviving colonies contains a gene called SUC2 that codes for convertase, an enzyme needed to metabolize sucrose. Mutations in the SNF gen
history of biology
How much time is required for a single cell of Detoxivicatium completeium to grown into a population large enough to fill the volume of the earth?
Explain Dietetics Dietetics has been defined as the science and art of feeding individuals based on the principles of nutrition. It can also be said to be the "science
Importance of Research in Nursing: It is the responsibility of the nursing profession to discover, verify, structure and restructure the professional knowledge through syst
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Nucleosides : compounds formed from a nitrogenousbase and a penstose sugar.
Q. Selection of foods for diabetic patients? Some handy guidelines for selection of foods for diabetic patients is presented next. You may be aware that some foods need to be
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