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Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.
Pulmonary edema occurs when movement of liquid from the blood to the interstitial space and/or into the alveoli exceeds the return of liquid to the blood and its drainage through t
Mas s Extinction - Since beginning many species evolved and got extinct (in fact only 1 % is living). Sometimes it occured suddenly at large scale, (most recently dinosa
Define Some Example of Acidic and Basic Buffer? Frequently used example of acidic and basic buffer includes: - Acidic buffer: a mixture of CH 3 COOH (acetic acid) + CH 3 C
Q. Management goals for diverticular disease? The management goals discussed above are basic to prevention of diverticulitis. However, diet therapy during diverticulitis may b
What does skin color in man shows?
Explain about functions of the lacrimal apparatus. Tear Secretion Aqueous tears are secreted by the main (reflex secretion) and accessory (basic secretion) lacrimal
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Pharynx: Pharynx is a funnel shaped tube about 13cm long that starts at the internal nares and extends to the level of the cricoid cartilage. It lies posterior to the n
Osmoregulation in Terrestrial Environment Earlier you have learnt about osmoregulation in aquatic environment. In this section, we shall study how the terrestrial animals cop
disadvantages of protozoa in points
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