Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

What are the main human blood group systems, What are the main human blood ...

What are the main human blood group systems? In humans the major blood group systems are the ABO system, the Rh system and the MN system.

Main species of taenias that cause human diseases, Q. What are the two main...

Q. What are the two main species of taenias that cause human diseases? The two chief species of taenias that cause disease in humans are Taenia solium, or the pork tapeworm, an

Explain about the toxicity of pyridoxine, Explain about the Toxicity of pyr...

Explain about the Toxicity of pyridoxine? Though toxicity related to pyridoxine intake are rare, but use of high doses of pyridoxine for the treatment of pre-menstrual syndrom

Importance of forests - fuel wood, Importance of Forests - Fuel Wood T...

Importance of Forests - Fuel Wood The forests are renewable resources which provide us a wide variety of commodities. For man, forests have been a source of recreation and the

What is placebo and nocebo, Problem 1: What is Placebo and Nocebo? Desc...

Problem 1: What is Placebo and Nocebo? Describe the significance of Placebo in clinical trials. Definition of placebo and Nocebo Significance of Placebo in clinical t

What do you know about tricuspid stenosis, Q. What do you know about Tricus...

Q. What do you know about Tricuspid stenosis? It is rare disease, with rheumatic etiology seen in 90 per cent of cases. In patients with rheumatic mitral stenosis only 3-5 per

Inorganic nitrogen and sulphur metabolism, Inorganic Nitrogen and Sulphur M...

Inorganic Nitrogen and Sulphur Metabolism Productivity in agriculture, forestry and other ecosystems is basically limited by the availability of nitrogen nutrients like nitrat

What is hemoglobin releasing hormone, In a red blood cell, which of the fol...

In a red blood cell, which of the following will lead to a reduction in the percentage of hemoglobin that has oxygen bound to it? A. A decrease in the amount of HbRH (Hemoglobi

basic phases of pcr, What are three basic phases of PCR?  Which phase of t...

What are three basic phases of PCR?  Which phase of these three phases is used for analysis for the end-point PCR? Why? Which phase is used for analysis for the quantitative real-t

How can a great biological diversity protect an ecosystem, How can a great ...

How can a great biological diversity protect an ecosystem from environmental damage? Why are less biodiverse ecosystems at risk of suffering deep biological harm if submitted to ev

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd