Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

#virus, how do retroviruses reproduce?

how do retroviruses reproduce?

To test the gas given off when seeds germinate, To test the gas given off w...

To test the gas given off when seeds germinate Place some mustard seeds in a flask with some damp cotton wool, in the apparatus shown in the diagram, and permit them to germina

Describe class diplopoda and chilopoda in detail, Describe Class Diplopoda ...

Describe Class Diplopoda and Chilopoda in detail? Members of the Subphylum Crustacea and the Subphylum Uniramia have one major characteristic in common. Both groups have biting

What is teratology, Teratology: It is the study of abnormal embryos. In ot...

Teratology: It is the study of abnormal embryos. In other words teratology is the study of abnormalities of physiological development. It is often use as the study of birth defect

Gastrulation process - formation of primitive streak, Gastrulation Process ...

Gastrulation Process - Formation of Primitive Streak Gastrulation in all amniotes involving eutherian mammals is related to a characteristic structure termed as the primitive

Define nutritional management of food allergies, Define Nutritional Managem...

Define Nutritional Management of Food Allergies and Food Intolerance? The term "hypersensitivity" is general and may include true allergies, reactions that do not affect the im

Explain about the adolescent pregnancy, Explain about the Adolescent Pregna...

Explain about the Adolescent Pregnancy? The pregnant adolescent is viewed as a high-risk patient, highly susceptible to suboptimal pregnancy outcome. Even when not pregnant, a

Define application of nutrition knowledge in geld of sports, Define Applica...

Define Application of Nutrition Knowledge in Geld of Sports? By the time you are ready to do this unit, you have been exposed to a good deal of the basic knowledge of nutrition

Describe what is respiration in dynamic auscultation, Describe what is Resp...

Describe what is Respiration in Dynamic Auscultation ? a) S 2 : Splitting of S 1 is audible during inspiration. b) Diastolic sounds and Ejection sounds: Inspiration augment

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd