Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.
what type of skull does amphibians possess in general?
Imaginal Discs In the holometabolous larva, there are two cell populations: (1) The larval cells that are used for the larval structures and (2) The imaginal disc and th
Name the high-energy complex generated during the conversion of succinyl CoA to succinate in the citric acid cycle GTP or ATP is the high-energy complex generated during th
Explain the Cardiovascular Effects? You must have heard of the 'French Paradox'. The lower incidence of heart attacks in certain areas of France despite higher prevalence of fa
Q. Is the epithelium vascularized? How do oxygen and nutrients reach the epithelium? Why is this feature an important evolutionary acquisition? Epithelia are not vascularized c
Strobilation - Types of Asexual Reproduction Strobilation is a type of asexual reproduction in which successive segments are separated off from the body one after other. It is
Hexokinase, that catalyzes the first irreversible step of glycolysis, is inhibited by glucose 6-phosphate. Thus when PFK is inhibited and fructose 6-phosphate builds up an
Q. How Linnaeus classify the plant kingdom? Linnaeus classified the plant kingdom into 24 classes in his famous work 'Genera Plantarum' (1737) and 'Species Plantarum' (1753). I
Mammals - Regeneration in Vertebrates Mammals are not capable of regenerating limb or tails. However, there are a few exceptions where lost tissues are regenerated. The annual
Define Corneal Ulcer - Micronutrient Deficiencies? Corneal xerosis, if not treated promptly, leads to ulceration of the cornea. Initially, the ulcer may be shallow, and if it b
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd