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Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.
Conformational Protection - Azotobacter In conformational protection a Fe-S redox protein provides protection to the enzyme. The protein gets oxidised in the presence of oxyge
Describe the Timing of Surgery and Intervention For Various Congenital Heart Diseases ? The timing of surgical or trans-catheter intervention for congenital heart disease (CHD
Preventive steps for global warming : Growing trees in vacant places. Growing social forests and protect them. Promoting abundant growth of phytoplankton plant species in marine
Q. Explain Inborn Errors of Metabolism? Some of the disorders caused by inborn error of metabolism namely phenylketonuria, tyrosinemia, maple syrup urine disease, homocystinur
a) What is symbiotic nitrogen fixation? b) Name the two protein components required for this process. Define their role.
Number of Implants The use of more implants decreases the number of pontics and the associated mechanics and strains on the prosthesis, and dissipates stresses more effectively
Why do climbers such beans plants coil up a post?
African horse sickness African horse sickness (AHS) is a highly fatal insect born viral disease of equidae caused by an Orbivirus (family Reoviridae) and characterized by sever
define histon protein?
. which is the main part of respiratory organ?
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