Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

Genetics, 5. Maple syrup urine disease is a rare inborn error of metabolism...

5. Maple syrup urine disease is a rare inborn error of metabolism. It derives its name from the odor of the urine of affected individuals. If untreated, affected children die soon

Determine the diversity of biological evolution, Is crossing over important...

Is crossing over important for the diversity of biological evolution? Sexual reproduction and recombination of linked genes (crossing over) are, with mutations, the main instru

What is the prothallus of pteridophytes, Q. What is the prothallus of pteri...

Q. What is the prothallus of pteridophytes? The gametophyte develops by mitosis from a spore and Prothallus is the pteridophyte gametophyte (the haploid individual that forms g

Endothecium - anther wall layers, Endothecium - Anther Wall Layers The...

Endothecium - Anther Wall Layers The layer of cells lying immediately next to the epidermis is the endothecium, which is responsible for the dehiscence of the anther. It is us

Explain the dietary management of a lactating mother, Explain the Dietary M...

Explain the Dietary Management of a lactating mother? Meeting the needs of lactation requires a varied nutrient-dense diet. Generally, a well-balanced diet will meet nutrient n

Protozoa., brief account on classification and general characters of protoz...

brief account on classification and general characters of protozoa

Role of endarterectomy, Role of Endarterectomy :  Endarterectomy is done a...

Role of Endarterectomy :  Endarterectomy is done as a semi open technique. 5-10mm long incision is made on the outer layers of the coronary artery and plain developed in 11ic thic

Define measuring direct aerobic fitness, Define Measuring Direct Aerobic Fi...

Define Measuring Direct Aerobic Fitness (VO 2 max) For direct measurement of VO, max, the subject is asked to perform exercise for 12 to 15 minutes. A stationary bicycle or a

Define measuring body composition- total body potassium, Define measuring b...

Define measuring body composition- Total body potassium? It applies the' concept that body cells contain a constant amount of potassium and that its measurement is theref

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd