Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

What is the water cycle, What is the water cycle? The water cycle repre...

What is the water cycle? The water cycle represents the circulation and recycling of water in nature. Liquid water on the planet surface is heated by the sun and turns into

Preet, what is microbiology

what is microbiology

What is the large size of cephalopods, Q How is the large size of some ceph...

Q How is the large size of some cephalopods related to the kind of circulatory system they present? In cephalopods the circulatory system is closed and this provides more press

Becoming online tutor, how can i work with u as an online biology tutor?

how can i work with u as an online biology tutor?

Signify the term - analogy, Signify the term - Analogy, Analogous? Refe...

Signify the term - Analogy, Analogous? Refers to structures that do not have the same evolutionary origin but have the same functions. The wings of a bat and insect are analogo

Explain clinical manifestations of cardiomyopathy, Q. Explain Clinical Mani...

Q. Explain Clinical Manifestations of cardiomyopathy? 1) Asymptomatic, when the diagnosis is made by 2D Echocardiography. 2) Enlargement of LV. Apex is shifted down and out.

Explain about the vitamin d, Explain about the Vitamin D? Vitamin D is ...

Explain about the Vitamin D? Vitamin D is a generic term and indicates a molecule of the general structure with  rings (A, B, C, D), as you may have noticed in Figure B. The ri

Erythrocytes or red blood cells, Erythrocytes or red blood cells: Th...

Erythrocytes or red blood cells: These are otherwise called Red Blood cells or corpuscles. These are the most numerous cells in the blood.There are about 4.5 to 5.5x 10 9

Describe how to prepare 200 ml of naoh, Describe how you would prepare 200 ...

Describe how you would prepare 200 ML of a 0.25 Msoluation of NaOH from a 3 M stock solution?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd