Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

What are the advantages of intra oral periapical radiographs, Advantages of...

Advantages of Intra Oral Periapical Radiographs a) It is a useful high yield modality for ruling out local bone or dental disease b)  It is of value in identifying critical

A plant growing on a mountainside, What two abiotic factors might affect (a...

What two abiotic factors might affect (a) an animal living at the bottom of the sea, (b) a plant growing on a mountainside?   (a) The abiotic factors which might

Recycling of plasma membrane components, Recycling of Plasma Membrane Compo...

Recycling of Plasma Membrane Components During exocytosis secretory vesicles fuse with plasma membrane, adding to the membrane surface area. Yet, the surface area of plasma me

What are the features of retrograde peri-implantitis, The Features of Retro...

The Features of Retrograde Peri-Implantitis include: - Periapical Bone loss without gingival inflammation. - Microflora consisting mainly of streptococci and nonmotile organ

Differentiate between glycogenesis and glycogenolysis, Differentiate betwee...

Differentiate between glycogenesis and glycogenolysis. The synthesis  of glycogen in liver and muscle  is  called glycogenesis.  The breakdown of glycogen in the liver (glycoge

Technique of operation, Technique of Operation :  TEE probe i...

Technique of Operation :  TEE probe is passed in all cases soon after anaesthesia. The initial steps and exposure of mitral valve are done as for open mitral valvotom

Tuberculosis, T ub e rc u l o si s Tuberculosis in animal is ca...

T ub e rc u l o si s Tuberculosis in animal is caused by Mycobacterium bovis , M. tuberculosis and M. avium and among these the most important causal agent is M.

What do you understand by global warming, Q. What do you understand by glob...

Q. What do you understand by global warming? The Global warming is the increase in the temperature of the planet due to accumulation of some gases in the atmosphere, especially

Adaptations in skeleton for upright posture, ADA PTATIONS IN SKELETON FOR ...

ADA PTATIONS IN SKELETON FOR UPRIGHT POSTURE - 1.      Foramen magnum is directed downward so that the head may rest vertically on the vertebral column. 2.      Four curves

Maternity and child health & family planning, M A TERNIT Y AND CHILD HEA...

M A TERNIT Y AND CHILD HEALTH & FAMILY PLANNING - 1.       The health centers give necessary information, guidance & assistance to mother before & after delivery pregnant wo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd