Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

Define some precautions for estimation of amount of bacteria, Define some P...

Define some Precautions for Estimation of the Amount of Bacteria? 1. Dilution should be made carefully. 2. Use fresh sterile pipette for making each dilution. 3. Aseptic

Define tracheal system in details., Define tracheal system in details. ...

Define tracheal system in details. Respiratory system found in insects and some spiders where air passes directly to the tissues through a series of tubules. Since oxygen tran

Proteins - digestion process, Proteins are very essential macromolecules as...

Proteins are very essential macromolecules as they have a range of many important functions like: • Required for building and repair of body tissues (including muscle) • Enzy

Sedge-meadow stage - hydrarch, Sedge-Meadow Stage - Hydrarch Favoured ...

Sedge-Meadow Stage - Hydrarch Favoured by an increasing amount of light, as the former occupants disappear, they gradually change the reed swamp into a sedge meadow. And now s

Fuel requirements - deforestation, Fuel Requirements - Deforestation T...

Fuel Requirements - Deforestation The increasing demand for fuel wood is one of the major factors leading to the degradation of the forest ecosystem. Fuel wood is of such majo

Effect of genetic drift, Is the effect of genetic drift likely to be the sa...

Is the effect of genetic drift likely to be the same in pop 1 and pop 2? How are genetic drift and pop size related? when there is strong selection against the homozygous recessive

Define standard titration for estimation of reducing sugar, Define Standard...

Define Standard Titration for estimation of reducing sugar by Fehling Soxhlet Method? Pipette 5 ml of Fehling's A and 5 ml of Fehling B solution into a 100 ml conical flask. Ad

Explain syphillis, Syphillis  Parenteral penicillin G remains the drug ...

Syphillis  Parenteral penicillin G remains the drug of choice for treating all stages of syphilis. Primary, secondary or latent syphilis known to be of less than one year's dur

Common features of eye and limb development, Common Features of Eye and Lim...

Common Features of Eye and Limb Development A comparison of the embryonic development of the very much different organs, the eyes and the limb, points to various interesting

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd