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Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.
Explain the senses taste and smell ? The pigmented cone cells of the retina transmit information to bipolar nerve cells, then to ganglion cells, and on to the optic nerve and t
Explain Deficiency diseases due to vitamin C? Vitamin C deficiency in human's results in the disease called scurvy, whose symptoms include hemorrhaging (especially in the gums)
Black quarter Synonym: BlacklegBlack-quarter (BQ) is an acute infection but a non-contagious disease characterized by inflammation of skeletal and cardiac muscles, severe toxae
Which of the following best defines the reasons why the sliding clamp is able to confer processivity on DNA polymerase and not DNA primase? A. The sliding clamp coats the templ
Purifying antigens present in small quantities as a mixture by relating an antibody to an antigen.
Permissive Interaction - Pancreas Development Figure describes the development of pancreas in a mammal. In an organism like mouse the pancreas is first seen as a diverticulum
#questiocan you help in a biology question?.
In continuous wave Doppler, the transducer has two Doppler crystals, one to constantly transmit and one to continuously receive. As a result of this continuous mode of tra
What is Deletion of cell? A deletion is simply when a tip or fragment of a chromosome breaks off and fails to reattach itself to the chromosome from which it came. The gene, or
What is the Speech Perception Test The subject is asked to listen to a series of 60 sounds, each of which consists of a double e digraph with varying prefixes and suffixes. The
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