Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

Nutritional management for constipation, Q. Nutritional management for cons...

Q. Nutritional management for constipation? Calories, proteins, carbohydrates and fat the requirements would be the same as the RDA for a particular age, sex, occupation of t

BIOLOGY , HOW CAN YOU JUSTIFY NEWTON THIRD LAW OF MOTION IN A TYPICAL PLANT...

HOW CAN YOU JUSTIFY NEWTON THIRD LAW OF MOTION IN A TYPICAL PLANT CELL?

Mollusca, General characters of mollusca

General characters of mollusca

Canine parvovirus infections, Canine parvovirus infections The canine p...

Canine parvovirus infections The canine parvovirus infections are caused by a virus, which belongs to the genus Parvovirus in the family Parvoviridae. This virus is similar to

Definition of rabies, Question 1 Explain various methods employed for ...

Question 1 Explain various methods employed for the lab diagnosis of rabies Definition of rabies Listing of various methods employed for rabies Explaining the meth

Which does not support evolution, Which of the following does not support e...

Which of the following does not support evolution? biogeography the fossil record comparative anatomy radiometric dating All of these choices are evidence of/support evolution.

Explain cephalization in nematodes and platyhelminthes, How is the nervous ...

How is the nervous system characterized in beings of the phylum Annelida? How can one compare cephalization in annelids to cephalization in nematodes and platyhelminthes? Annel

#title.circulatory system, #question.what is the mechanism of circulatory s...

#question.what is the mechanism of circulatory system .

Explain about the supply curve or supply schedule, Explain about the supply...

Explain about the supply curve or supply schedule. The supply curve or schedule shows the total quantity of a good or a service supplied within aggregate, by whole sellers or f

What phrase best describes oxidation- reduction reaction, What phrase best ...

What phrase best describes oxidation- reduction reaction? A. phosphoryrylation energezing endergounic reactions b. transferring of electrons from one molecule to another c. shar

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd