Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

Maedi/visna, Maedi/visna Maedi, a progressive pneumonia, and visna, a n...

Maedi/visna Maedi, a progressive pneumonia, and visna, a neurotropic disease, found in sheep and goats are caused by the Maedi/visna virus, belonging to the family Retroviridae

Explain the feeding process for preschoolers, Explain the Feeding process f...

Explain the Feeding process for Preschoolers? The word preschool children, you may be aware, is used for children less than six years of age. After the first year, there is a s

Respiration, different types of animals and their respiratory organ

different types of animals and their respiratory organ

The concentration of the tissue fluid, The concentration of the tissue flui...

The concentration of the tissue fluid, which bathes all cells in the body, is kept more or less constant. Why is this important? If the tissue fluid became more dilute, the ce

Procedure for estimation of vitamin c - colorimetric method, Define Procedu...

Define Procedure for estimation of vitamin c - colorimetric method? Carry out the experiment following the instructions given herewith: 1. Prepare a series of tubes for blan

Blood groups, BLOO D GROUPS- In R.B.C. on plasmalemma antigen or agglu...

BLOO D GROUPS- In R.B.C. on plasmalemma antigen or agglutinogen present form antibodies or agglutinines. Blood groups are based on antigens. 1 .      ABO SYSTEM -

Describe a method of diagnosing diabetes mellitus, Q. In ancient Greece the...

Q. In ancient Greece the father of Medicine, Hypocrates, described a method of diagnosing diabetes mellitus by tasting the patient's urine. What is the physiological explanation fo

What do you meant by medical writing, Question 1 : What do you meant by ...

Question 1 : What do you meant by medical writing? Show various types of medical writing. Define and explain briefly medical writing Discuss various types of medical w

Explain significance of mitosis for embryonic development, Q. What is the s...

Q. What is the significance of mitosis for the embryonic development? Every embryo grows from a single cell that bears mitosis and generates other cells that also divide themse

Does mitosis properly occur before or after the interphase, Does mitosis pr...

Does mitosis properly occur before or after the interphase? Is it a mere "point of view" issue? Mitosis must be considered a succeeding phase after interphase since this

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd