Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

Strawberry slain - common disorders of skin, Strawberry Slain: It  sig...

Strawberry Slain: It  signfies the dilatation  of deeper vessels  to form  cavernous spaces like those seen  in  the erectile tissue of the penis. The  lesion  is well-defined

What is plutonium reprocessing, What is plutonium reprocessing? Why is it a...

What is plutonium reprocessing? Why is it a big environmental issue? Plutonium is the highly radioactive chemical element produced from uranium by nuclear plants. Plutonium can

What is the structural depiction of a carboxyl group, Q. What is the struct...

Q. What is the structural depiction of a carboxyl group? Carboxyl groups have a carbon attached to one hydroxyl group by a simple bond and one oxygen by a double bond. The othe

Explain about the lignans, Explain about the Lignans? Lignans are diphe...

Explain about the Lignans? Lignans are diphenolic compounds formed by dilnerization of 2 cinnamic acid residues. Most lignans apparently pass through the GIT as fibre. Some lig

Kangroo rat, Kangroo Rat Kangroo rat Dipodornys merriami, a native of ...

Kangroo Rat Kangroo rat Dipodornys merriami, a native of South-West America is a classical example of how small mammals survive in desert. It exhibits all the osmoregulatoryad

Determine the members of kingdom plantae, Normal 0 false fals...

Normal 0 false false false EN-IN X-NONE X-NONE

Sensitive/critical periods: windows of enhanced plasticity, There are speci...

There are specific sensitive/critical periods in development during which acquisition of certain skills is substantially easier than at other times. In the animal domain there are

Describe pharmacologic agents in amyl nitrite, Describe Pharmacologic Agent...

Describe Pharmacologic Agents in amyl nitrite ? Amyl Nitrite : It produces vasodilatation which initially results in marked fall in BP and in the next 30-60 seconds there is

What is plant coleoptile, What is plant coleoptile? Why does the removal of...

What is plant coleoptile? Why does the removal of the coleoptile extremity disallow plant growth? Coleoptile is first (one or more) aerial structure of the sprouting plant that

Determine the diversity of biological evolution, Is crossing over important...

Is crossing over important for the diversity of biological evolution? Sexual reproduction and recombination of linked genes (crossing over) are, with mutations, the main instru

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd