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Nuclear run-on is a technique used to estimate the relative rate of the transcription of a given gene, as opposed to steady-state level of the mRNA transcript (which is influenced not just by the transcription rates, but by the stability of the RNA). This method is based on the assumption that the highly-transcribed gene should have more molecules of RNA polymerase bound to it than will the same gene in the less-active state. If it is properly prepared, isolated nuclei will continue to transcribe genes and incorporate 32P into RNA, but only in those transcripts which were in progress at the time the nuclei were isolated. Once the polymerase molecules complete transcript they have in progress, they should not be able to re-initiate the transcription. If that is true, then amount of the radiolabel incorporated into a specific type of mRNA is theoretically proportional to the number of RNA polymerase complexes present on that gene at the time of isolation. A very difficult method, rarely applied appropriately from what I understand
detail study of coelenterata
Primary Succession - Community Change In primary succession on a terrestrial site the new site is first colonised by a few hardy pioneer species that are often microbes, liche
Q. What are the major orders of placental mammals? What are a few representative species and distinguishing features of each of those orders? The orders into which placental ma
C o l l i ba c i ll o si s It is commonly seen in newly born farm animals and occurs in septicaemic and enteric collibacillosis forms. E tiology: The disea
Define Types of Root Canal Perforations According To Causes Iatrogenic causes. Pathological causes.
Why is water conservation in c3 c4 and cam plants important? Cam stands for Crassulacean acid metabolism. C3 and C4 conserve less water than Cam plants. Actually, C4 plan
Response to Heavy Metal Stress Several heavy metals emanating from industrial mining and sewage disposal operations contaminate the environment. Cadmium is a common contamina
Black quarter Synonym: BlacklegBlack-quarter (BQ) is an acute infection but a non-contagious disease characterized by inflammation of skeletal and cardiac muscles, severe toxae
Write a DNA palindrome or inverted repeat of at least 10 nucleotides in length.
Types of gastrulation
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