Nuclear run-on, Biology

Assignment Help:

Nuclear run-on is a technique used to estimate the relative rate of the transcription of a given gene, as opposed to steady-state level of the mRNA transcript (which is influenced not just by the transcription rates, but by the stability of the RNA). This method is based on the assumption that the highly-transcribed gene should have more molecules of RNA polymerase bound to it than will the same gene in the less-active state. If it is properly prepared, isolated nuclei will continue to transcribe genes and incorporate 32P into RNA, but only in those transcripts which were in progress at the time the nuclei were isolated. Once the polymerase molecules complete transcript they have in progress, they should not be able to re-initiate the transcription. If that is true, then amount of the radiolabel incorporated into a specific type of mRNA is theoretically proportional to the number of RNA polymerase complexes present on that gene at the time of isolation. A very difficult method, rarely applied appropriately from what I understand


Related Discussions:- Nuclear run-on

What are SOL, What are sols?  A colloidal system in which solid  partic...

What are sols?  A colloidal system in which solid  particles are dispersed in a liquid is referred to as a sol, to distinguish it from a true solution. In a true solution, the

Cell, what is cell and what are the components of cell

what is cell and what are the components of cell

Explain the term bleeding, Bleeding Bleeding from injury to small blood...

Bleeding Bleeding from injury to small blood vessels pushed along the lateral wall of expanding maxillary sinus should be contained by bone wax or electrocautery rather than cr

Nitrogen cycle, Nitrogen Cycle The nitrogen is an essential constituen...

Nitrogen Cycle The nitrogen is an essential constituent of protein - the building block of all living cells. It is also a major constituent of the atmosphere (79 per cent). Al

Animal around us, #question.what are the reason that arthropodans are abund...

#question.what are the reason that arthropodans are abundant in nature.

Anatomy, what is the longest cell in human body

what is the longest cell in human body

What is meant by substrates of enzymatic reactions, Substrates are reagent ...

Substrates are reagent molecules upon which enzymes act. The enzyme has spatial binding sites for the attachment of its substrate. These sites are known as activation centers of

What do you mean by hansens disease, Q. What is Hansen's disease (etiologic...

Q. What is Hansen's disease (etiological agent, mode of transmission, clinical manifestations and prevention)? The etiological agent of the Hansen's disease is bacteria called

Guidelines for diagnosis and treatment planning, Guidelines for diagnosis a...

Guidelines for diagnosis and treatment planning The diagnosis and planning the case is by far the most important step in the entire sequence of the treatment and due considerat

Types of blood vessel, Types of Blood Vessel Arteries Arteries ca...

Types of Blood Vessel Arteries Arteries carry blood away form the heart via the pulmonary artery and aorta. Arterioles As major arteries begin to branch , th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd