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Nuclear run-on is a technique used to estimate the relative rate of the transcription of a given gene, as opposed to steady-state level of the mRNA transcript (which is influenced not just by the transcription rates, but by the stability of the RNA). This method is based on the assumption that the highly-transcribed gene should have more molecules of RNA polymerase bound to it than will the same gene in the less-active state. If it is properly prepared, isolated nuclei will continue to transcribe genes and incorporate 32P into RNA, but only in those transcripts which were in progress at the time the nuclei were isolated. Once the polymerase molecules complete transcript they have in progress, they should not be able to re-initiate the transcription. If that is true, then amount of the radiolabel incorporated into a specific type of mRNA is theoretically proportional to the number of RNA polymerase complexes present on that gene at the time of isolation. A very difficult method, rarely applied appropriately from what I understand
Osmoregulation in Marine Non-Chordate Metazoans Studies on the osmotic pressure of body fluids of marine organisms have displayed that their internal osmotic pressure is more
Ground level ozone: Ground level ozone is the ozone present at the lower level of earth atmosphere. It is not emitted directly into the environment but produced by complex chemi
Explain the Polycarbonate Nucleopore Filtre? A given sample is filtered through a membrane filter, usually polycarbonate nucleopore filter with a pore size that will retain bac
Q. Signs of constrictive pericarditis? 1) Jugular venous pressure (JVP) is elevated in all patients of CP which reflects the elevated diastolic pressure of the right atrium.
Composition of blood: Take few ml. of fresh blood in a test tube and before collecting the blood add a few drops of sodium oxalate or sodium citrate solution to prevent clot
what three connective tissues are liquid
Excluding the effects of cardiac output and hormones, describe the other factors that may affect blood pressure and blood flow in a middle-aged man who is exercising in an aerobics
Urea molasses mineral block (UMMB) licks Development of urea molasses mineral block licks is another technology being increasingly used in several parts of India and such lick
how the blood is circulated with the help of heart
Describe the Intensity of S 2 in second heart ? Loud A 2 occurs in patients with hypertension, when aorta is closer to anterior chest wall owing to root dilatation or TGA or
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