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Nuclear run-on is a technique used to estimate the relative rate of the transcription of a given gene, as opposed to steady-state level of the mRNA transcript (which is influenced not just by the transcription rates, but by the stability of the RNA). This method is based on the assumption that the highly-transcribed gene should have more molecules of RNA polymerase bound to it than will the same gene in the less-active state. If it is properly prepared, isolated nuclei will continue to transcribe genes and incorporate 32P into RNA, but only in those transcripts which were in progress at the time the nuclei were isolated. Once the polymerase molecules complete transcript they have in progress, they should not be able to re-initiate the transcription. If that is true, then amount of the radiolabel incorporated into a specific type of mRNA is theoretically proportional to the number of RNA polymerase complexes present on that gene at the time of isolation. A very difficult method, rarely applied appropriately from what I understand
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A fruit fly has four pairs of chromosomes in its cells. At meiosis, how many dissimilar combinations of maternal and paternal chromosomes are possible in the gametes? In gamet
Explain Nutritional Support Management for radiation therapy? For managing these patients on radiation therapy, the following measures can be under taken: 1. Administration
Kingdom Plantae The kingdom plant are includes multicellular eukaryote organisation with photosynthetic nutrition. Typically cell has cellulose wall, sap vacuole, plastids and .
Determine uses of Soybean protein? Soybean protein concentrates have been used as stabilized dispersions in milk- like beverages and simulated dairy products, such as sour crea
Define water losses by visible perspiration? The water losses by visible perspiration are highly variable; the amount could be as high as 4L in hot climate or during strenuous
Primary Succession - Community Change In primary succession on a terrestrial site the new site is first colonised by a few hardy pioneer species that are often microbes, liche
Q. Enlist the modalities available for hand wash? Modalities available for hand wishing are a) 2-4% Chlorhexidine b) 0.3-1 %Triclosan c) 0.6% Parachlorometaxylenol (PC
a) Name three parts of the body where smooth (involuntary or unstriated) muscle may be found. b) In any one of your examples, say what the smooth muscle does.
Denaturation: With respect to the nucleic acids, refers to the conversion from the double-stranded to single-stranded state, many times achieved by heating or alkaline conditions.
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