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You are working at a large, well funded research institute with the latest biochemical techniques available. The focus of your research is to find a specific mutation, or set of mutations, that lead to differential expression of the mutated gene in different tissues. For a variety of reasons, you cannot, or do not wish to use micro-array technology. So, you choose to start by measuring by some other means the mRNA levels in a variety of tissues.
What is a good combination of techniques that will allow you to rapidly quantify the amounts of multiple RNA species and to give some indication of the amounts of specific messages (transcripts of genes)? You do have the ability to do many types of PCRs and also have an assortment of DNA probes that are known to effectively hybridize to regions of mRNAs that may contain the mutated regions of interest.
Define Distribution of body iron in different compartments? In humans, the total quantity of iron in the body varies with haemoglobin concentration, body weight, gender and the
Describe how enzymes assay is helpful in milk and meat Wheat seeds a) Determination of α-amylase, content: an increase indicates sprouting/ germination of stored wheat;
Why d scientists pick hydrogen as the basis for MRI scanning? Name of the human body that do not appear in an MRI scan. Explain thermal stratification? How does thermal stratifi
"Diversity is good" is one of the axioms of conservation biology. However in absentia of historical information, why is noting higher diversity in a system NOT the best way to eval
Explain Skeletal muscle Skeletal muscle has low activity of glucose-6-phosphate dehydogenase. Yet, like most other tissues it can synthesize ribose-5-phosphate. This is.
living and non living
Cell wall is the structure produced by some of the cells outside their cell membrane; variously composed of the chitin, peptidoglycan, or cellulose.
Endoplasmic reticulum (ER) is the network of membranous tubules in the cytoplasm of a cell; involved in production of the phospholipids, proteins, and the other functions. Rough E
Define Absorption, Storage and Elimination of cyanocobalamin? Vitamin B 12 in food is bound to proteins and is only released by the action of a high 'Concentration of hydroch
What is End-to-End Anastomosis with Subclavian Flap Aortoplasty ? The two operations can be combined as a single procedure. Medially end-to-end anastomosis is done between the
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