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You are working at a large, well funded research institute with the latest biochemical techniques available. The focus of your research is to find a specific mutation, or set of mutations, that lead to differential expression of the mutated gene in different tissues. For a variety of reasons, you cannot, or do not wish to use micro-array technology. So, you choose to start by measuring by some other means the mRNA levels in a variety of tissues.
What is a good combination of techniques that will allow you to rapidly quantify the amounts of multiple RNA species and to give some indication of the amounts of specific messages (transcripts of genes)? You do have the ability to do many types of PCRs and also have an assortment of DNA probes that are known to effectively hybridize to regions of mRNAs that may contain the mutated regions of interest.
Metameric segment - Segmentation On the other hand true metameric segment as best observed in annelids has separate schizocoelic body cavity of mesodermal origin in each segme
What is Metabolism ? The sum total of vital activities ( movements , nutrition, growth, respiration, excretion , reproduction, behaviour ,etc. Is the life of a living bei
Protraction from HIV Under the following conditions HIV is not transmitted 1) The secretions and body fluids like Saliva, Tears, Sweat, Faeces, and Urine have negligible HIV c
What factors lead to spoilage of fish flesh? A difference in the composition of tissues among different species, climate, procurement and holding practices are amongst few of
Q. What is action mechanism of antibiotic penicillin? Penicillin, discovered by the Scottish doctor Alexander Fleming in 1928, is a drug that inhibits enzymes essential for the
Unprecedental clarity has come to our understanding of genetic differences by the analysis of DNA sequences. It is not surprising in which the latest DNA technologies are leading t
what the by products of sugarcane
DIFFERENCE BETWEEN PLANTS AND ANIMALS - S . N o . C h a ra c ter P l an ts A n i m al s 1 Nutrition
Structure of Blastula The structure of blastula turns into modified in several animal groups. The changes are related to the amount of yolk deposited in the egg, as you will s
Cortex can be described as follows 1) The outer part of the organ, such as, the adrenal cortex, which produces many steroidhormones; 2) in plants, the area of the stem or root
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