Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Morphogenesis and Totipotency
A chlamydomonas mother cell gives rise to two daughter cells by a simple division. But a leaf cell cannot give rise to a new plant except in cases like Bryophyllum or Kalanchoe. What makes a daughter cell of a zygote to loose the 'potential' to develop into a whole plant? Or if all the daughter cells possess this potential- i.e. if they are totipotent-why it does not express? These questions troubled Haberlandt-hundred years ago. He postulated that any living plant cell should be able to grow into a full plant. He experimented with mesophyll cells at a time when tissue culture had not been unheard for. His experiments failed because we now know that isolated mesophyll cells are not really easy to grow to unleash their morphogenetic potential.
F.C. Steward-who took up this work sixty years later-successfully cultured whole carrot plants from carrot phloem parenchyma cell. He published the papers in the American Journal of Botany in the late 1950's. Steward and his coworkers took 2 mg. tissues of secondary phloem of carrot roots and grew them in special flasks with nipples or tubes called tumble tubes. The medium was whites' medium with coconut water. These tubes/flasks were mounted on a wheel that was rotated on a shaft at the rate of 1 r.p.m. So that the pieces were alternately aerated and bathed in the liquid medium. There was an enormous increase in size of the explants. A callus ensued. A few peripheral cells slaughtered off into the medium and started dividing and gave rise to clumps,-occasionally with roots these could be transferred to semi-solid medium in (still) tubes. Shoots arose opposite the roots to yield full plants. Subsequently other parts were also used to, demonstrate cellular totipotency.
Triacylglycerols are synthesized from fatty acyl CoAs and glycerol 3-phosphate.The glycolytic intermediate dihydroxyacetone phosphate is first reduces to glycerol 3-p
Amelioration of mineral deficiency Performance of livestock in the tropics is mainly governed by the quality and quantity of nutrients provided in the diet. In most of the dev
Which one of the following statements about morula in humans is correct? 1. It has almost equal quantity of cytoplasm as an uncleaved zygote but much more DNA 2. It has far l
Alcohol Related Emergencies: Alcohol is one of the most serious health problems. Alcoholism is defined as a primary or chronic disease with genetic, psychological and environ
What is the name of enzyme in gastric juice?
Explain Procedure for Gram Staining of Bacterial Cultures? Now carry out the exercise following the steps enumerated herewith. 1. Prepare bacterial smear on a clean, non-gre
Natural and Artificial Ecosystem i) Natural ecosystems are those which are mostly free from human disturbances, such as tropical forests, grasslands, oceans, lakes and deserts.
Determination of Limb Polarities The limb pattern is characterized by three axes (or polarities) as displayed in diagram: (1) The direction from the shoulder (or hip) to t
Explain Keshan disease caused by Selenium deficiency? It is a cardiomyopathy (disease of the myocardium, involving heart muscle) that was identified to affect children and wome
Advantage of solar energy: 1. It is renewable: it produce comes from sun, so it is plentiful and will always be there as long we need it . 2. Solar
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd