Methods including quantification of rna species, Biology

Assignment Help:

1.   Northern blotting

The quantity of rna species can be quantified by calculating the quantity of rna through northern blotting which provide size and sequence information regarding the rna molecule.

In this technique a sample of rna is separated by an agarose gel and hybridise to a radioactive rna probe that is corresponding to target sequencer. The radio labeled rna is then identified through an autoradiograph.

2.    Reverse transcriptase-real time quantitative pcr

The other technique for quantification of mrna is rt-qpcr, in this technique reverse transcription creates a dna template from the mrna, this single stranded template is known as cdna. The c dna template is amplified through rt-qpcr in a quantitative step during which fluorescence emitted through labeled hybridization probes or intercalating dyes variates as dna amplification process proceeds. With the standard curve qpcr can create an absolute measurement of the number of copies of real rna.

3.   Hybridisation micro array

This method comprises of single array or chip that include probes to know transcript level for every known gene in the genome therefore assist in quantification of rna species.

4.  Serial analysis of gene expression and rna sequencing are tag based methods which can give a relative measurement of the cellular concentration of diverse rna.

 


Related Discussions:- Methods including quantification of rna species

What do you meam by parthenogenesis, Q. What is parthenogenesis? Parthe...

Q. What is parthenogenesis? Parthenogenesis is the formation or reproduction of a new individual from the egg cell but without fecundation by the male gamete. According to the

Ecology, does jellyfish depend on solarenergy

does jellyfish depend on solarenergy

Structure of the vascular cambium, Structure of the Vascular Cambium T...

Structure of the Vascular Cambium This meristem, in the anatomical sense of the term usually includes two histologically distinct kinds of cells: i) Fusiform Initials, i

Determine the assess mobility of prosthesis, Assess Mobility of Prosthesis ...

Assess Mobility of Prosthesis All implant-supported prostheses must be stable.  To detect mobility, place an explorer or scaler under the embrasures and apply gentle pressure i

Hyphae and the mycelium of pluricellular fungi, Q What are the hyphae and t...

Q What are the hyphae and the mycelium of pluricellular fungi? The major structures of pluricellular fungi are the hyphae threadlike filaments made of contiguous uni or multinu

Formation of new membranes, Formation of New Membranes Divisions of t...

Formation of New Membranes Divisions of the egg or blastomeres raise the total surface area of the two daughter cells to be covered via membrane at every cleavage. The parent

What are the amylase inhibitors, What are the Amylase Inhibitors? Inhib...

What are the Amylase Inhibitors? Inhibitors of the enzyme a-amylase are found in wheat, rye, beans, mango, legumes, potatoes, sorghum (jowar) and oats. Most amylase inhibitors

Types of axons, Types of Axons Axons are of two types Mye...

Types of Axons Axons are of two types Myelinated and Non-myelinated In the case of myelinated axons the neurilemma is ensheathed by another cover

Describe coronary spasm, Q. Describe Coronary Spasm? Usually spasm deve...

Q. Describe Coronary Spasm? Usually spasm develops at the site of subcritical or critical stenoses, but it may also occur in angiographically normal coronary arteries, the so c

Invertebrate zoology, give an example invertebrate phylum/phyla for protopl...

give an example invertebrate phylum/phyla for protoplasmic grade organization

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd