Method to estimate phytochrome, Biology

Assignment Help:

Method to Estimate Phytochrome

Since there is no chemical test, the only method available to test and estimate phytochrome is the spectrophotometric method. Firstly, the difference in the absorbance between 660 and 730 nm is measured after red light irradiation (Δ OD red, No. 1.). Next, the sample is irradiated with far-red light and again the difference in Δ OD is measured (Δ OD far-red, No. 2). The content of total phytochrome is measured by subtracting the value obtained after red light irradiation from far-red light irradiated value.

2343_Method to Estimate Phytochrome.png

Nowadays special, spectrophotometers are also available which can measure the absorption difference between two wavelengths. These are called dual wavelength spectrophotometers.


Related Discussions:- Method to estimate phytochrome

Define ascorbic acid content of white blood cells, Define Ascorbic acid con...

Define Ascorbic acid content of white blood cells? The ascorbic acid content of white blood cells and platelets is a good index of the tissue levels of ascorbic acid. The level

Energetic function of organic molecules, Q. What are the few examples of th...

Q. What are the few examples of the energetic function of organic molecules? Ans. Since they are complex molecules, organic molecules store large amount of energy, present

How can we assess the nutritional status of a chd infant, How can we assess...

How can we assess the nutritional status of a CHD infant? Various assessment methods can be used, particularly anthropometrical -weight, length (height), head circumference and

Skeletal system - arm bones, ARM BONES - Each arm contain 30 bones as ...

ARM BONES - Each arm contain 30 bones as : humerus in upper arm, radius ulna in forearm, 8 carples in wrist, 5 metacorples  in plam, 14 phalnges in fingers. HUMERU

Races of modern man, RACE S OF MODERN MAN Australoids Negroids ...

RACE S OF MODERN MAN Australoids Negroids Caucasoids Monogoloids American Indian Polynesian All human races have the same number of chromosomes (46) and b

What is the major cell organelle involved in cell digestion, Q. What is the...

Q. What is the major cell organelle involved in cell digestion and what are the properties of that organelle that enable it to do the task? The organelles accountable for intra

What is counselling for diabetic patient, Q. What is counselling for diabet...

Q. What is counselling for diabetic patient? The word counselling has been understood in a number of ways. Counselling can be understood as "consultation, mutual interchange

What is the constitution of the cartilaginous matrix, What is the constitut...

What is the constitution of the cartilaginous matrix? The cartilaginous matrix is made of collagen fibers, mostly collagen type II, and of proteoglycans, proteins associated to

Membrane that selects based on pore size, A transport process in which phys...

A transport process in which physical (hydrostatic) pressure drives part of a fluid through a membrane that selects based on pore size

Distribution of stomata, Distribution of Stomata There are two paramet...

Distribution of Stomata There are two parameters normally used for expressing the distribution of stomata. Stomatal frequency: The number of stomata per unit area

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd