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During our discussion of replication origins we took a step-by-step look at the method of cloning DNA fragments into plasmids. Based on your knowledge of this discussion which of the experimental manipulations would lead to the failure to insert a fragment of genomic DNA into the plasmid vector?
A. Digest plasmid DNA with the BamH1 restriction enzyme and genomic DNA with the EcoR1 restriction enzyme.
B. Add bacteria that have been transformed with a plasmid having the kanamycin (antibiotic) resistance gene to an agar plate that having chloramphenicol (antibiotic).
C. DNA polymerase is not added to the ligation mixture.
D. Add bacteria that have been transformed with plasmid DNA to a plate that lacks an antibiotic.
State the process of metabotropic receptors Consider a system that contains two neurons and one cardiac SA node cell in a culture dish bathed in normal physiological saline. A
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Q. What are the two major morphological patterns of cnidarians? Concerning locomotion how do these forms differentiate from each other? Morphologically, cnidarians classify as
Mollusks
Looking at what the academic world is publishing, microarray research is actually hot right now, and people haven't quite figured out what the best way is (some might argue if we s
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Define about the Resistant Starch? Until 1980's starch was thought to be completely hydrolyzed and absorbed from the small intestine of man, independent of its source, type and
Biologists established that new cells Consequent to this doctrine, biologists established that new cells are formed by cell division and by the end of the nineteenth century, t
What is the term for the pattern of base pairing between one DNA strand and its partner in a duplex?
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