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During our discussion of replication origins we took a step-by-step look at the method of cloning DNA fragments into plasmids. Based on your knowledge of this discussion which of the experimental manipulations would lead to the failure to insert a fragment of genomic DNA into the plasmid vector?
A. Digest plasmid DNA with the BamH1 restriction enzyme and genomic DNA with the EcoR1 restriction enzyme.
B. Add bacteria that have been transformed with a plasmid having the kanamycin (antibiotic) resistance gene to an agar plate that having chloramphenicol (antibiotic).
C. DNA polymerase is not added to the ligation mixture.
D. Add bacteria that have been transformed with plasmid DNA to a plate that lacks an antibiotic.
Ecotone - Nature and Structure of Community The zone of vegetation separating two different types of communities is called ecotone. It is also known as a transition zone. The
Thickness and toughness of plant walls are increased by deposition within original cellulose wall, of a substance called as lignin. Process of deposition is called: a) Lignific
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Cell culture products: Animal cell cultures are used to generate virus vaccines, as well as different kind of useful biochemical which are mainly high molecular weight prote
skeletal system of fish .exoskeleton and endoskeleton
(a) Bedroom : · Do not place room heaters near any inflammable materials. · Do not use heaters
how nematodes adapt to their mode of feeding
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4 The lac operon is an
The gene is a distinct portion of a cell's DNA. Genes are coded instructions for making all the body needs, mainly proteins. Human beings have about 25,000 genes. Researchers have
Ecosystem diversity measures based on the species composition of flora, or fauna, or other ecosystem properties, are all equally justifiable: but measures based on different crite
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