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The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane. This is commonly achieved by adding a detergent that solubilizes the membrane. In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS. they readily intercalate into the lipid disrupt & bilayer the hydrophobic interactions as the detergent molecules are themselves amphipathic .
figure: Structures of (a) Triton X-100 and (b) octyl glucoside.
figure: (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.
Write about embryonic stem cell invitro methord in which transgenic animal were generated and efficient show how homologous recombination happening?
Who is the founder of theory survival of the fittest ?
Cat scratch disease It is caused by Bortenella henselae, a gram-negative, non-motile bacterium. The organism is found in all parts of the world. The cat is an important reserv
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Discuss about the Common migraine This is the most frequent type, occurring in more than 80% of migraine sufferers. There is no clear aura as there is in classic migraine, but
Of which type of tissue is the heart made? How is this tissue oxygenated and nutrified? The heart is made of striated cardiac muscle tissue. The heart muscle is known as the my
How does a plasma membrane regulate movement of molecules into and out of a cell?
Roots have no chlorophyll and grow in darkness. So how do roots obtain their food? Food made in the leaves is transported to the roots in the phloem of the vascular bundles.
A lk a l in e indigestion The disease occurs due to the consumption of excessive amount of protein rich concentrate or non protein nitrogenous substance like urea, and is
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