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The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane. This is commonly achieved by adding a detergent that solubilizes the membrane. In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS. they readily intercalate into the lipid disrupt & bilayer the hydrophobic interactions as the detergent molecules are themselves amphipathic .
figure: Structures of (a) Triton X-100 and (b) octyl glucoside.
figure: (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.
Genetic engineering has been successfully used for producing: 1. Transgenic mice for testing safety of polio vaccine before use in humans 2. transgenic models for studying ne
Q. Write the meaning of diabetes mellitus? The word "diabetes" is from the Greek word meaning "a siphon". Diabetes patients had polyuria (passing excessive urine) and "pass it
A laboratory mouse homozygous for an RFLP marker is mated to a wild mouse that is heterozygous for that marker. One of the heterozygous individuals resulting from this cross is mat
Q. What is the structure that to maintains identical chromatids bound? The structure that to maintains identical chromatids bound is the centromere.
Kingdom Animalia Multicellular, heterotrophic, eukaryotes, tissues are specialised and most of them have organs, mostly highly responsive. Only gametes are haploid, fertilisation
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Q. In which meiotic division does the separation of identical chromatids take place? And After the end of this process what are the ploidies of the new cells? The separation of
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