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The first step of the purification of an integral membrane protein is to disrupt its interactions with other integral proteins and the lipids in the membrane. This is commonly achieved by adding a detergent that solubilizes the membrane. In the order to solubilize membrane but not denature the protein mild detergents like as octyl glucoside or Triton X-100 is used rather than the harsh detergent SDS. they readily intercalate into the lipid disrupt & bilayer the hydrophobic interactions as the detergent molecules are themselves amphipathic .
figure: Structures of (a) Triton X-100 and (b) octyl glucoside.
figure: (a) Detergent solubilization and (b) reconstitution into artificial lipid vesicles of an integral membrane protein.
Simple sugars are monosaccharides consisting of a single polyhydroxy aldehyde or ketone unit. Their general formula is (CH 2 0) n (n=3 to 7).
Define Some Example of Acidic and Basic Buffer? Frequently used example of acidic and basic buffer includes: - Acidic buffer: a mixture of CH 3 COOH (acetic acid) + CH 3 C
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Restriction endonucleases are enzymes which: 1. make cuts at specific positions within the DNA molecule 2. recognize a specific nucleotide sequence for binding of DNA ligase
If a solution has a pH of 7.5, what would its new pH be if the concentration of H 3 O ions in the solution were increased by 100 times? Explain your reasoning. As a tenfold inc
Explain the Turbidity Measurement? Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estim
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