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• Cyclins accumulate during the G1, S, and G2 phases of the cell cycle. • By the G2 checkpoint (the red bar in the figure), enough Cyclin is available to form MPF complexes (aggregations of Cdk and Cyclin) which initiate mitosis.
o MPF apparently functions by phosphorylating key proteins in the mitotic sequence.
• Later in mitosis, MPF switches itself off by initiating a process which leads to the destruction of Cyclin
o Cdk, the non-Cyclin part of MPF, persists in the cell as an inactive form until it associates with new Cyclin molecules synthesized during Interphase of the next round of the cell cycle.
When the R-wave in the lateral precordial leads is less than 10 mm the sensitivity of ST depression is very low if 1 mm of ST depression is used as a standard. The corrected ST for
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