Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Light microscopy:
Microscopy started with light microscopes using visible light to illuminate the objects, the most used and familiar microscope is called compound light microscope. Its simplest form it consists of two optical lenses, one at each of a hollow tube. The lens closer to the object is called objective and the one closer to the observer eyes is called eyepiece. The object, mounted upon a glass slide, which is put upon a centrally perforated satge under the objective, is illuminated by light , a mirror is fitted below the stage to focus light on to the object, a third lens system ,called condenser may be fitted between the mirror and the stage to concentrate the light. The limit of the resolving power of best compound light microscopes is about 0.2 achieving a magnification of about 2000 times the size of the objects. Thus these microscopes can resolve even most of the bacteria, but they cannot reveal the internal details of bacterial cells.
Use of ground glasses as optical lenses to magnify objects was presumably realized several centuries ago. The first name on record is of Conrad Gesner who used magnifying lenses to observe certain foraminifera's. Next Zacharias Janssen (Dutch 1590) constructed, together with his father, Hans Janssen the first compound light microscope to study insects which could magnify objects 10 to 30 times. Galileo constructed microscope with greater magnifying powers and studied the arrangement of facets in the compound eyes of insects. Marcello Malpighi (1628-1694) microscopically studied animal and plant tissues in thin slices of several organs, earning the title of the father of microscopic anatomy, Robert Hooke (English 1665) designed a compound light microscope either a magnifying power of about 42 times and studied amongst other object thin slice of cork discovering cells. Antony van Leeuwenhoek (Dutch 1676) designed single lens microscopes with magnifications approaching 300 times and become the first to observe sperms, blood cells muscle fibres, lenses of eyes, hydrae yeast cells and microscopic organisms in water like bacteria, protozoan's rotifers tec. Earning the little of the father of microbiology so commonly used these days by students in schools and collages all over the world , was developed from Hooke model by Wilson (1970).
Zigmondy (1900) designed an ultra microscope using ultraviolet rays in place of ordinary light and quartz lenses in place of ordinary glass lenses, achieving a resolving power of0.1 and magnification of about 4000 times.
Alterations occurring in meat and poultry Meat, as you already know, is rich in proteins and contains most of the essential amino acids. It is also rich in minerals such as c
Q. Dietary tips for hyperlipideinic patients? It is important to note here that the dietary modifications need to be individual in each patient and these should be tailored lo
Composition of blood: Take few ml. of fresh blood in a test tube and before collecting the blood add a few drops of sodium oxalate or sodium citrate solution to prevent clot
What is Gene Expression ? Gene expression is the process by which a gene is activated to produce a certain protein. Cells are able to respond to changes in the environment i
Heat Production In the Homeotherms heat production must be elevated if the ambient temperature falls below the critical temperature. Although all metabolic processes result i
What is the valve that separates the aorta from the heart? What is the importance of that valve? The valve among the left ventricle and the aorta is the aortic valve. The aorti
Is nitrogen is important plant nutrients Nitrogen is an important plant nutrient which is assimilated by most of the plants as nitrate and ammonium ions. Organic matter is the
E m u l s io n products Ground meat products such as sausages, patties and luncheon meats are emulsion based products and their quality depends upon the ability of lean m
What are the three major groups into which mammals are divided? The three groups into which mammals are divided are: monotremes (or prototherian, e.g., platypus), marsupials (o
Planning the Nursing Care 1) Maintain hydration and electrolyte balance. 2) Administer accurate and adequate antibiotics drugs. 3) Prevent the spread of infection.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd