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You may have heard in the news currently that a new species of frog was discovered within a rainforest.
You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.
Questions:
a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.
b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.
Male reproductive disorders due to infections Septicemic infections have a greater affinity for vesicular glands than other regions of the reproductive tract viz. prostate, am
Biochemical Changes Many workers have tried to follow the biochemical changes that precede flowering and result in meristems which give rise to flowers instead of vegetative s
5 beneficial effects of decomposers and 2 harmful effects
Ganglia - Organisation of Nervous System In between higher non-chordates with a central nervous system, you will observe that the association neurons and motor neurons are con
What is Glands? The central nervous system controls much of the endocrine system through the hypothalamus. The hypothalamus directs many of the body's functions through the "ma
Posterior Maxilla Posterior maxilla has very poor quality of bone demanding special consideration in implant placement: Increasing the number of implants to decrease the cr
Define the term - Acoelomate Triploblastic animals which do not have an internal body cavity. This includes flatworms and ribbon worms. However the term could be applied to oth
Early Pre-operative Care 1. Admission of patient-usual admission procedure to be completed. 2. Assessment of the patient. 3. History 4. Physical examination
Describe the structure of alginate The commercially available alginates are white to cream coloured powders. 1% alginic acid solution gives a pH value of 2.9 while it is 2.3 an
(a) What carbohydrates does a plant make from glucose? (b) Which of these carbohydrates is transported round the plant? (c) Which carbohydrate is the main s
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