Isolate genomic dna, Biology

Assignment Help:

You may have heard in the news currently that a new species of frog was discovered within a rainforest.

You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.

Questions:

a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.

b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.


Related Discussions:- Isolate genomic dna

Define nutritional requirement in cold and polar environment, Define Nutrit...

Define Nutritional Requirements in Cold and Polar Environment? Energy requirements are the major consideration for providing nutritional support in a cold environment. Energy e

Where can rna be found within cells, Where can RNA be found within cells? ...

Where can RNA be found within cells? In the eukaryote cell nucleus RNA can be found dispersed in the nuclear fluid, with DNA, and as the main constituent of the nucleolus. In c

Foraminiferans - protozoan, Foraminiferans - Protozoan Foraminiferans ...

Foraminiferans - Protozoan Foraminiferans are largely benthic marine species. They have multi chambered calcareous tests or shells with numerous pores, hence the name foramini

Transcription and processing of prokaryotic rrna, In E.  coli  there  are  ...

In E.  coli  there  are  seven  rRNA  transcription  units  scattered  by  the genome,  each of that contains one copy of every of the 16S, 23S  and 5S rRNA genes and one to four c

Selenium, S e l e n i u m Selenium toxicity due to pollution of ...

S e l e n i u m Selenium toxicity due to pollution of environment has been reported in animals. Contamination of pasture from industries emitting fly ash particularly fo

Why is the cytoplasm of sperm cells very reduced, Q. Why is the cytoplasm o...

Q. Why is the cytoplasm of sperm cells very reduced? Why do mitochondria of sperm cells concentrate in the base of the flagellum? The reduced cytoplasm of sperm cells reduces t

Larval forms - porifera, Larval forms - Porifera In the majority of sp...

Larval forms - Porifera In the majority of sponges the larval stage develops in the body of the parent. The larva is generally at the blastula stage of development. Figure dis

Subphylum sarcodiha, Subphylum Sarcodiha Pseudopodia typically presen...

Subphylum Sarcodiha Pseudopodia typically present; flagella present in developmental stages of some species; free living or parasitic. Superclass - Rhizopoda Loc

Waste Water, Why is there a concern on waste water?

Why is there a concern on waste water?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd