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You may have heard in the news currently that a new species of frog was discovered within a rainforest.
You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.
Questions:
a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.
b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.
Benefits of energy pyramids
Determine about the term - neuropsychology The clinical practice of neuropsychology involves an integration of knowledge bases from the disciplines of psychology, psychometrics
Functions of Citric Acid Cycle The citric acid cycle is an amphibolic pathway i.e. it is involved in both anabolic and catabolic processes
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Q. What is Intermediate acting insulin? Intermediate acting: This type works more slowly than short acting. It works hardest 8-12 hours after injection and still keeps on worki
Illustrate the theory of Goldman according to corneal transparency. Goldman's Theory: The fibrils are small in relation to light and do not interfere with light transmissi
drawandlabelthemajorendocrineglandsofthehumanbody
What is the window phase of an infection? How is this concept important for the test of HIV infection in blood banks? The primary immune response of the body facing any infecti
Explain for Procedure Quantitative Determination of Viable Microbes? Now carry out the exercise following the steps included herewith: 1. Label the diluent tubes from 10 -1
Soybean protein used as stabilized dispersions Soybean protein concentrates have been used as stabilized dispersions in milk-like beverages and simulated dairy products, such a
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