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You may have heard in the news currently that a new species of frog was discovered within a rainforest.
You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.
Questions:
a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.
b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.
What happens to a denatured enzyme regarding its functionality? How can that result be explained with the help of the lock and key model? According to the lock and key model th
DISACCHARIDES They are oligosaccharides composed of two monosaccharide residues . Three common disaccharides are sucrose (glucose + fructose) = cane suger maltose
What is Physiology and Hemodynamic ? Normally, in the post-natal circulation the pulmonary and aortic circuits are in series so that the deoxygenated blood coming into the righ
Working of Specific Acquired Immunity Primary lymphoid organs, as you already know, are thymus and bone marrow. Secondary organs are lymph nodes, spleen and lymphoid tissue. Wh
Q. Generally which phase of the cell cycle has longer duration? The mitotic phase has quite a shorter length and the interphase comprises approximately 4/5 of the cell cycle.
How is the circulatory system of reptiles characterized? What is the basic difference among the reptile and the amphibian heart? The circulatory system of beings of the class R
Obelia is considered to be of special interest in zoology as an animal showing an intermediate grade of organization?
Which of these lists contains the four postulates of natural selection? Which of these lists contains the four postulates of natural selection? Uniform population, heritable trait
Determine the Steps for Withdrawing the Insulin The important thing about measuring a dose is to use the front of the plunger not the back - Check doctor's prescription.
what is endosperm haustorium
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