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You may have heard in the news currently that a new species of frog was discovered within a rainforest.
You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.
Questions:
a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.
b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.
Whenever a chromosome is replicated the replication machinery passes by the nucleosomes without deleting the histones from the DNA. How this happens is since not fully understood.
Population Growth - Population Parameters and Regulation The size of a population depends upon the balance between natality and immigration through which individuals are added
Tapetum - Anther Wall Layers Tapetum is the innermost and most important layer of the anther wall. It is generally composed of a single layer of cells. These cells completely
Explain radiation sterilization Various studies have been conducted which show the effect of processing on vitamins especially, thiamine. In one study, which compared the effec
What is the difference between bryophytes and tracheophytes? Bryophytes are nonvascular plants (mosses, hornworts, liverworts), i.e., they do not have a conductive system for t
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Define Endurance capacity by standard running tests? Endurance capacity, on the other hand, is measured by standard running tests in which time to exhaustion is taken as the en
G L YCOLIPIDS They are lipids having sugar residues. Two common glycolipids are cerebrosides and gangliosides. (1) Cerebrosides They are made of amino alcohol sphi
Carbohydrates and proteins question #Minimum 100 words accepted#
Q. Show the Error Detection and Editing Function? Error Detection and Editing Function: Consciousness helps us avoid acting solely through habit. It allows us to make novel res
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