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A) Definitive Infective Endocarditis
1) Pathological Criteria
• Micro organism: demonstrated by culture or histology in a vegetation; or in a vegetation that has embolized, or in an intra cardiac abcess, or • Pathological lesions: vegetation or intracardiac abscess present, confirmed by histology showing active endocarditis.
2) Clinical Criteria, using specific definitions listed below:
• Two major criteria, or • One major and three minor criteria, or • Five minor criteria.
B) Possible Infective Endocarditis
• One major +1 minor or 3 minor.
C) Rejected
• Firm alternative diagnosis for manifestations of endocarditis, or • Sustained resolution of manifestations of endocarditis, with antibiotic therapy for 4 days or less, or • No pathological evidence of infective endocarditis at surgery or autopsy, after antibiotic therapy for 4 days or less.
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Blotting method to detect particular one proteins Ans) Western blotting
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