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There are three steps of PCR
a) Denaturation. The reaction combination is heated to 95°C for a short time period (about 15-30 sec) to denature the goal DNA into one strands which can act as templates for DNA synthesis.
b) Primer annealing. The combination is rapidly cooled to a described temperature that allows the two primers to bind to the sequences on each of the two strands flanking the goal DNA. This annealing temperature is measured carefully to ensure in which the primers bind only to the desired DNA sequences. Every primer binds to each strand. Two parental strands do not reanneal with each other because the primers are in huge excess over parental DNA.
c) Elongation. The temperature of the mixture is raised to 72°C (commonly) and kept at this temperature for a preset period of time to permits DNA polymerase to elongate each primer by copying the single-stranded templates. At the last of this incubation both single-stranded template strands have been made partially double stranded. The new strand of each double-stranded DNA extends for a variable distance downstream
The human body can exhibit a wide range of resistances to current depending on the path of the current, contact area, and sweatiness of the skin. Suppose the resistance across the
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Explain about the Food Science and Technology? Food Science and Technology are so inextricably linked that usually these are treated as one field of study. While Food Science d
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Indications For Surgery : The normal aortic valve area in the adult is about 3-4 cm2. Significant symptoms occur when the valve area is seduced to 1/4"' the normal area
Endocrine glands Endocrine glands have no ducts - so these are also called as ductless glands Eg.Pituitary etc., These glands secrete chemical substances called HORMONES
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What is the significance of magnesium for plants? What are the other main biological functions of magnesium? Magnesium is fundamentally significant for plants because it is par
Define Advantage & disadvantage of using Algae as source of protein? Advantage Produces proteins which have almost all the Essential Amino acids. Rich in tyrosine
PURPOSE OF EVALUATION: The purpose of evaluation of service is to answer the questions, "To what extent and how well did the programme meet the objectives of the service?'
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