Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
There are three steps of PCR
a) Denaturation. The reaction combination is heated to 95°C for a short time period (about 15-30 sec) to denature the goal DNA into one strands which can act as templates for DNA synthesis.
b) Primer annealing. The combination is rapidly cooled to a described temperature that allows the two primers to bind to the sequences on each of the two strands flanking the goal DNA. This annealing temperature is measured carefully to ensure in which the primers bind only to the desired DNA sequences. Every primer binds to each strand. Two parental strands do not reanneal with each other because the primers are in huge excess over parental DNA.
c) Elongation. The temperature of the mixture is raised to 72°C (commonly) and kept at this temperature for a preset period of time to permits DNA polymerase to elongate each primer by copying the single-stranded templates. At the last of this incubation both single-stranded template strands have been made partially double stranded. The new strand of each double-stranded DNA extends for a variable distance downstream
Growth and development of multi cellular organisms The normal growth and development in all multicellular organisms occur in a programmed manner. The new born babies have
protozoa locomotion
Simple diffusion Only comparatively small uncharged or hydrophobic molecules (H2O, O2, and CO2, other gases, urea and ethanol) cross the lipid bilayer through simple
Explain Mechanism of Absorption of Haem Iron? Haem iron is soluble in the alkaline environment of the intestine. It binds to the receptor on the enterocytes and is internalized
Pseudocoelom - Metazoa The platyhelminths which do not have a body cavity surrounding the gut, have a solid type of body constitution. The mesoderm completely fills the space
Agriculture - Degradation of Ecosystem Agriculture whether traditional or modern involves intervention and modification of the natural ecological systems. Ever since man has s
Q. Objective of nutritional management of hypertension? The objective of nutritional management of hypertension includes: • To achieve gradual weight loss in overweight and
1. By direct measurement of the activity of the thyroid gland in several patients who had received 131 I for diagnostic purposes, it was observed that the biological elimination h
2. Vaccinations against various childhood diseases have contributed to the entry of women, particularly mothers, into the fulltime workplace. a. Is this statement supported by data
Sir/Madam, I am a Banquet Farm Owner and face the problem with the Grass that in November & December the grass does not grow well or disappear beacuse of heavy foot fall. Is their
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd