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There are three steps of PCR
a) Denaturation. The reaction combination is heated to 95°C for a short time period (about 15-30 sec) to denature the goal DNA into one strands which can act as templates for DNA synthesis.
b) Primer annealing. The combination is rapidly cooled to a described temperature that allows the two primers to bind to the sequences on each of the two strands flanking the goal DNA. This annealing temperature is measured carefully to ensure in which the primers bind only to the desired DNA sequences. Every primer binds to each strand. Two parental strands do not reanneal with each other because the primers are in huge excess over parental DNA.
c) Elongation. The temperature of the mixture is raised to 72°C (commonly) and kept at this temperature for a preset period of time to permits DNA polymerase to elongate each primer by copying the single-stranded templates. At the last of this incubation both single-stranded template strands have been made partially double stranded. The new strand of each double-stranded DNA extends for a variable distance downstream
Based on your reading of the article "Origin of Life on Earth" which of the following is a false statement regarding how life developed? A. As proteins are needed for the repli
When I had predicted into class, we had a huge snow storm this weekend. Now what I want you to do are to engineer several grass for my yard therefore my grass never lets snow accum
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Explain Risk Assessment Risk Assessment : The scientific evaluation of known or potential adverse health effects resulting from human exposure to food borne hazards
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TYPES OF CELL WALL Cell wall differentiated into the following layers : 1. Primary Cell Wall 2. Secondary Cell wall 3. Tertiary Cell wall 1 .
State the definition of Bio-medical waste 'Bio-medical waste' means any solid and/or liquid waste including its container and any intermediate product, which is generated durin
Q. Describe about defecation reflex? An example of a reflex having an autonomic (and somatic) component is the DEFECATION REFLEX: The rectum is generally empty, but when fec
Cosmid: The type of artificially constructed vector taken in use for cloning the 35-45 kb of DNA. These are plasmids carrying the phage l cos site (which permits packaging into l
working of pcr
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