Illustrate about sterilization protocol, Biology

Assignment Help:

Q. Illustrate about Sterilization protocol?

Sterilization protocol includes proper transportation and cleaning of instruments followed by their packing. Items can be sterilized using modalities like dry heat such as flaming, or moist heat like pasteurization, boiling though highly unreliable as a sterilization technique and more effective for disinfection, it is used very often.  Autoclave means self locking and is used to denote an apparatus that sterilizes by use of steam under pressure. It operates on same principle as pressure cookers. Radiation is cold sterilization with no heat involved; non ionising radiation include infrared and UV rays. Lethal effect is through generation of heat and DNA damage. Penetration is low but efficacy is high. By chemicals such as alcohols: ethyl alcohol, isopropyl alcohol; aldehydes: formaldehyde: widely employed for the fumigation of operation theatres. Gutaraldehyde: commercial preparations are active at different pH and have percentage of 2-3.2. They can destroy microbes in 10 hours. Gases: ethylene oxide. The choice of opting for a particular mode of sterilization depends on the physical and chemical properties of the items to be sterilized. Proper sterilization needs to be coupled with aseptic storage envoirnment.

Second part of infection control includes operatory room protocol which starts with preparation of operatory followed by preparation of patient and the operator. For the operator  use of personal protective equipment (PPE) - clothing, masks, eyewear, and gloves and Hand washing is considered important measures to reduce the risk of transmitting organisms to patients and HCWs (health care workers). The purpose of washing is twofold: to reduce the both transient and resident flora for the duration of a procedure to protect the operator in the event that there is non-intact skin on the hand, thereby accomplishing the aim of maintaining the "chain of asepsis".


Related Discussions:- Illustrate about sterilization protocol

Biological significance of incompatibility, Biological Significance of Inco...

Biological Significance of Incompatibility Both interspecific and intraspecific incompatibility determines the degree of inbreeding and out breeding of plants. Self-incompati

What is the function of the vitellus in the vertebrate egg, Q. What is the ...

Q. What is the function of the vitellus in the vertebrate egg? How are these eggs classified according to the amount of vitellus within them? Vitellus (yolk) is the nutritive m

Objective of ecological study, The main objectives of study of ecology are:...

The main objectives of study of ecology are: (i)              To study the distribution of biota local and geographical level. (ii)             To study the interrelationship

Why do ribosomes move along mrna during translation, Why do ribosomes move ...

Why do ribosomes move along mRNA during translation? During translation the ribosome always exposes two mRNA codons to be translated by moving along the mRNA. When a peptide bo

Define direct measurement of individual''s work capacity, Define Direct mea...

Define Direct measurement of individual's Work Capacity? Direct measurement of VO 2 max is determined by having the subject perform exercise at increased loads, for 12 to 15 m

Define reagents required and methodology for benedict test, Define reagents...

Define reagents required and methodology for Benedict Test? Reagents Required - Sugar solutions of glucose, fructose, galactose, maltose, lactiose, sucrose and starch.

Explain counter stain - ziehl-neelsen method, Explain Counter Stain - Ziehl...

Explain Counter Stain - Ziehl-Neelsen Method? Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs c

Types of aortic stenosis-aortic stenosis-valve disease, Types of Aortic Ste...

Types of Aortic Stenosis:  Obstruction to left ventricular outflow is commonly at the valvar level. Less commonly it is at the sub valvar or supra valvar level. Sub valvar ob

Nutrition, The oxidation of sugar in the cell of higher organisms takes pla...

The oxidation of sugar in the cell of higher organisms takes place in the where

How many computers can access the ebook on, I usually study in the library ...

I usually study in the library on the schools computers so I will be accessing the eBook from many different computers throughout the semester. Is there a limit to how many compute

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd