Illustrate about sterilization protocol, Biology

Assignment Help:

Q. Illustrate about Sterilization protocol?

Sterilization protocol includes proper transportation and cleaning of instruments followed by their packing. Items can be sterilized using modalities like dry heat such as flaming, or moist heat like pasteurization, boiling though highly unreliable as a sterilization technique and more effective for disinfection, it is used very often.  Autoclave means self locking and is used to denote an apparatus that sterilizes by use of steam under pressure. It operates on same principle as pressure cookers. Radiation is cold sterilization with no heat involved; non ionising radiation include infrared and UV rays. Lethal effect is through generation of heat and DNA damage. Penetration is low but efficacy is high. By chemicals such as alcohols: ethyl alcohol, isopropyl alcohol; aldehydes: formaldehyde: widely employed for the fumigation of operation theatres. Gutaraldehyde: commercial preparations are active at different pH and have percentage of 2-3.2. They can destroy microbes in 10 hours. Gases: ethylene oxide. The choice of opting for a particular mode of sterilization depends on the physical and chemical properties of the items to be sterilized. Proper sterilization needs to be coupled with aseptic storage envoirnment.

Second part of infection control includes operatory room protocol which starts with preparation of operatory followed by preparation of patient and the operator. For the operator  use of personal protective equipment (PPE) - clothing, masks, eyewear, and gloves and Hand washing is considered important measures to reduce the risk of transmitting organisms to patients and HCWs (health care workers). The purpose of washing is twofold: to reduce the both transient and resident flora for the duration of a procedure to protect the operator in the event that there is non-intact skin on the hand, thereby accomplishing the aim of maintaining the "chain of asepsis".


Related Discussions:- Illustrate about sterilization protocol

Metameric segment - segmentation, Metameric segment - Segmentation On ...

Metameric segment - Segmentation On the other hand true metameric segment as best observed in annelids has separate schizocoelic body cavity of mesodermal origin in each segme

What is cryopreservation, Name the location and function of Meibomian gland...

Name the location and function of Meibomian glands in the human eye. a) What is cryopreservation? Give its single use. b) Commercial importance of cryopreservation is relate

Cleavage - development biology, Cleavage - Development Biology Cleavag...

Cleavage - Development Biology Cleavage or segmentation is a series of cell divisions of the fertilized .egg through which it is converted into a multicellular structure, call

Explain pure culture techniques, Explain Pure Culture Techniques We lea...

Explain Pure Culture Techniques We learnt the techniques involved in sub-culturing, i.e., the process involved in transfer of culture from one medium to another or transfer of

Simple precautions to prevent injuries, When resheathing needles, simple pr...

When resheathing needles, simple precautions to prevent injuries are to: 1. use only one hand to scoop the cap 2. hold the sheath with a hemostat (suggested but will require

Respiratory system of the phylum annelida characterized, Q. How is the resp...

Q. How is the respiratory system of beings of the phylum Annelida characterized? Respiration in annelids can be branchial or cutaneous. Cutaneous respiration takes place due to

What is the function of keratin in the epidermis, What is the function of k...

What is the function of keratin in the epidermis? The epidermis is the outer layer of the skin made of epithelial tissue. In the epidermis there are keratin-secreting cells (ke

Conventional cabg on cardiopulmonary bypass, Conventional CABG on Cardiopul...

Conventional CABG on Cardiopulmonary Bypass   Chest is opened by midline incision and median sternotomy. Simultaneously saphenous vein or radial artery is harvested. 'T

Applications of various technologies in biotechnology, Question 1: Brie...

Question 1: Briefly explain the applications of various technologies in biotechnology. Definition of biotechnology Brief on different applications of various technolo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd