Illustrate about sterilization protocol, Biology

Assignment Help:

Q. Illustrate about Sterilization protocol?

Sterilization protocol includes proper transportation and cleaning of instruments followed by their packing. Items can be sterilized using modalities like dry heat such as flaming, or moist heat like pasteurization, boiling though highly unreliable as a sterilization technique and more effective for disinfection, it is used very often.  Autoclave means self locking and is used to denote an apparatus that sterilizes by use of steam under pressure. It operates on same principle as pressure cookers. Radiation is cold sterilization with no heat involved; non ionising radiation include infrared and UV rays. Lethal effect is through generation of heat and DNA damage. Penetration is low but efficacy is high. By chemicals such as alcohols: ethyl alcohol, isopropyl alcohol; aldehydes: formaldehyde: widely employed for the fumigation of operation theatres. Gutaraldehyde: commercial preparations are active at different pH and have percentage of 2-3.2. They can destroy microbes in 10 hours. Gases: ethylene oxide. The choice of opting for a particular mode of sterilization depends on the physical and chemical properties of the items to be sterilized. Proper sterilization needs to be coupled with aseptic storage envoirnment.

Second part of infection control includes operatory room protocol which starts with preparation of operatory followed by preparation of patient and the operator. For the operator  use of personal protective equipment (PPE) - clothing, masks, eyewear, and gloves and Hand washing is considered important measures to reduce the risk of transmitting organisms to patients and HCWs (health care workers). The purpose of washing is twofold: to reduce the both transient and resident flora for the duration of a procedure to protect the operator in the event that there is non-intact skin on the hand, thereby accomplishing the aim of maintaining the "chain of asepsis".


Related Discussions:- Illustrate about sterilization protocol

Do all mammals have a placenta, Q. Do all mammals have a placenta? Mamm...

Q. Do all mammals have a placenta? Mammals of the monotreme group (platypus, echidnas) are oviparous egg- laying and they don't have a placenta. Mammals of the marsupial group

Define triglycerides, Q. Define Triglycerides? Triglycerides are produc...

Q. Define Triglycerides? Triglycerides are produced by the esterification of glycerol with three fatty acid molecules. They are the body's major energy store, particularly in a

Genetic, 1. The genes for ruby eyes (rb), tan body (t) and cut wings (ct) a...

1. The genes for ruby eyes (rb), tan body (t) and cut wings (ct) are all found on the X-chromosome of Drosophila melanogaster. All of these are recessive traits. They map in the or

Explain glucokinase, Glucokinase In the fed state, clearance of blood  ...

Glucokinase In the fed state, clearance of blood  glucose is mainly by  liver via glucokinase. Glucokinase, which  is an  inducible enzyme, removes most of the blood glucose fr

Show some examples of migratory animals, Q. What are some examples of migra...

Q. What are some examples of migratory animals? Instance of the migratory animals are southern right whales from Antarctica, that procreate on the Brazilian coast; the migrator

Metabolic cataysis, Metabolic Cataysis A large number  of chemical reac...

Metabolic Cataysis A large number  of chemical reaction  occur  every  moment in a cell  to keep it. In the living  state, The rate  if  chemical  reaction normally depends upo

Heterotropic nutrition, what is the meaning of heterotropic mode of nutrit...

what is the meaning of heterotropic mode of nutrition. with examples.

Assessment of aplastic anaemia, Assessment   The patient will present w...

Assessment   The patient will present with striking pallor, irritability and lethargy due to anaemia, bledding or bruising and petechiae due to thrombocytopenia, fever and infe

Indicator organism, a) What are the needsfor an organism to be an indicator...

a) What are the needsfor an organism to be an indicator organism? b)  In Australia, National Parks and coastal beaches attract various visitors during holiday periods.  EPA is

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd