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A restriction map of plasmid and a human gene of identified sequence is demonstrated below. Your job is to clone the human liver cDNA for that gene than the start codon is closer to Eco RI site than Pst I site of the polylinker, nevertheless get rid of the promoter+RBS+Hin insert already into the plasmid. Describe how you would accomplish the cloning by using a series of numbered steps.
Map of plasmid and a human gene
Remember that in the plasmid, there (1) refers to the frequency of such sites in the plasmid plus existing insert. Into the gene’s restriction map, each the relevant restriction sites are specify d when they are present.
Describe Surgical Closure of Patent Ductus Arteriosus technique? The patient is positioned in right lateral position and a left posterolateral thoracotomy is done. In infants
DIFFERENC E BETWEEN LAMARCKISM AND NEO-LAMARCKISM - S.No Lamarckism Neo-Lamarckism 1. It refers of the original
Explain the Carbohydrates and Fat Requirements during Sepsis? Carbohydrates should constitute nearly 60% to 70% of the total energy. Glucose is the primary calorie substrate in
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Open Pulmonary Valvotomy : Open pulmonary valvotomy by technique of inflow occlusion is done without cardio pulmonary bypass. Surface hypothermia is used and inflow occlusio
human genome project
TRANSPLAN T PROCEDURE - Operation is done under general anaesthesia, duration 3-4 hrs. Cut is given in the lower abdomen. Donar's kidney is transplanted retroposistonicaly
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