How you would accomplish cloning using of numbered steps, Biology

Assignment Help:

A restriction map of plasmid and a human gene of identified sequence is demonstrated below. Your job is to clone the human liver cDNA for that gene than the start codon is closer to Eco RI site than Pst I site of the polylinker, nevertheless get rid of the promoter+RBS+Hin insert already into the plasmid. Describe how you would accomplish the cloning by using a series of numbered steps.

1791_Map of plasmid and a human gene.png

Map of plasmid and a human gene

Remember that in the plasmid, there (1) refers to the frequency of such sites in the plasmid plus existing insert. Into the gene’s restriction map, each the relevant restriction sites are specify d when they are present.


Related Discussions:- How you would accomplish cloning using of numbered steps

Describe the term - ambulacral groove, Describe the term - Ambulacral groov...

Describe the term - Ambulacral groove The groove that runs down the oral surface of each echinoderm arm and contain the tube feet. If the region contains a visible furrow, or gr

Agro industrial-phenolic compounds, Phenolic compounds Gossypol: Gos...

Phenolic compounds Gossypol: Gossypol is a toxic compound found in the cotton plant. It is concentrated in the cottonseed but can also be found in other parts of the cotton

Excretion in prawn, EXCRETION IN PRAWN- A pair of antennary gland or gr...

EXCRETION IN PRAWN- A pair of antennary gland or green gland and a median renal sac present. Green glands lie at the base of antennae & open out by renal aperture.

Newcastle disease / ranikhet disease (nd/rd), N e wcastle disease / Ranik...

N e wcastle disease / Ranikhet disease (ND/RD) This disease is caused by Newcastle disease virus (NDV) belonging to the genus Paramyxovirus of the family Paramyxoviridae. Alo

Define translation phase of gene expression process, Define Translation Pha...

Define Translation Phase of gene expression process? The NDA which the transcript into m RNA is translated into protein with the help of ribosomes, m RNA, amino acids, a number

Explain the concept of dressing, Explain the concpet of Dressing Dressi...

Explain the concpet of Dressing Dressing: Means medicine wound covering; a bandage or other sterile covering is put on a wound to protect it from infection or further damage.

Acid indigestion, Acid indigestion It is also known as ruminal acidosis...

Acid indigestion It is also known as ruminal acidosis, acute carbohydrate engorgement, rumen over load or lactic acidosis. Ingestion of large quantities of highly fermentable c

Define absorption, Define absorption, transport and excretion of magnesium?...

Define absorption, transport and excretion of magnesium? Magnesium absorption to some extent is similar to that of Ca. Absorption of Mg occurs throughout the small intestine, a

Objective of nutritional management of hypertension, Q. Objective of nutrit...

Q. Objective of nutritional management of hypertension? The objective of nutritional management of hypertension includes: • To achieve gradual weight loss in overweight and

How can we keep our brain healthy in older age, Is there anything I can do ...

Is there anything I can do to keep my brain healthy in older age? It is increasingly clear that how we live our lives on a daily basis strongly influences how well our brain ag

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd