How much template dna will you add, Biology

Assignment Help:

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add


Related Discussions:- How much template dna will you add

Determine the theory of evolution, What is the problem that the theory of e...

What is the problem that the theory of evolution and its rival theories try to solve? The problem that the theory of evolution, or simply evolution, and its rival theories try

Find out which allele is dominant, Diagram the cross and determine which al...

Diagram the cross and determine which allele is dominant for each of the following Drosophila matings? a. Two red-eyed flies yield 110 red-eyed and 35 brown-eyed offspring.!

Explain human cells that produce proteins for exportation, What are some ex...

What are some examples of human cells that produce proteins for exportation? Which cytoplasmic organelle is expected to be well-developed and abundant in those cells? Specializ

Define prevention of idd - double fortified salt, Define prevention of idd ...

Define prevention of idd - Double Fortified salt? iron deficiency anaemia and iodine deficiency disorders often co-exist, the most effective approach to control these public he

What is the way of life of sponges, Q What is the way of life of sponges? ...

Q What is the way of life of sponges? Sponges live exclusively in an aquatic environment and they are attached by their base to a substrate fixation ground. Sponges are filteri

How the allele frequencies in a population, A dominant allele will always i...

A dominant allele will always increase in frequency over time. Yes No Q44. When a gene has two alleles, the allele frequencies in a population automatically move toward an equilibr

Reproductive cycles, Normal 0 false false false EN-IN ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Define the sun and solar drying method, Define the Sun and Solar Drying Met...

Define the Sun and Solar Drying Method? The make use of solar energy to preserve the food items is mainly the oldest form of food reservation and is still practiced today for f

What emergency department after an automobile accident, An 22 year old man ...

An 22 year old man is admitted to the emergency department after an automobile accident. He has not lost a large amount of blood, but he suffers from a severe crush injury to hi

What is iris coloboma, What is Iris Coloboma Coloboma is a congenital a...

What is Iris Coloboma Coloboma is a congenital abnormality due to non-fusion of the embryonic choroidal fissure. It can affect any part of the eye and may involve the optic ner

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd