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You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add
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Describe the Intensity of S 2 in second heart ? Loud A 2 occurs in patients with hypertension, when aorta is closer to anterior chest wall owing to root dilatation or TGA or
Substrates are reagent molecules upon which enzymes act. The enzyme has spatial binding sites for the attachment of its substrate. These sites are known as activation centers of
What is gluconeogenesis? Highlight its significance giving any two examples Gluconeogenesis (i.e synthesis of new glucose) is the synthesis of carbohydrate from non-carbohydrat
Q. What is the treatment process of mitral stenosis? Presence of symptoms or any complications is an indication for treatment. Apart from penicillin prophylaxis no other treatm
Q. Explain Spoilage of pasteurized milk? You would recall that pasteurization is one of the techniques to control and destroy the pathogenic, as well as, .spoilage bacteria i
What are colonies and societies? Colonies are functional integrated aggregates produced by individuals of the same species. Colonies are often confused with a one individual. E
Minerals :- Iron Food Source Cereals, legumes, meat contamination from iron utensils and soil Nutritional Functional role Essential nutrient: Deficiency lead
What is the most accepted hypothesis about the origin of life on earth? How does it compare to the other main hypotheses? The heterotrophic hypothesis is the strongest and most
What is the respective importance of water, carbon and nitrogen for living beings? Water is the major solvent of living beings and it is essential practically for all biochemic
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