How much template dna will you add, Biology

Assignment Help:

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?


Related Discussions:- How much template dna will you add

Integumentary system - claws, CL A W S - In rabbit and other animals...

CL A W S - In rabbit and other animals claws present, made up of keratine or S. corneum. Dorsal plate is unguise & ventral plate is sub unguise . Helpful in digging

Health hazards with poor management of bio-medical waste, Health Hazards wi...

Health Hazards with poor management of Bio-medical waste Health hazards associated with poor management of Bio-medical waste are: - Injury from sharps to staff and waste han

What is cardiac catheterization, Q. What is Cardiac Catheterization? Ro...

Q. What is Cardiac Catheterization? Routine cardiac catheterization is not necessary in patients with mitral regurgitation. It may be done when there is discrepancy between cli

Can you explain about krebs cycle, Q. Why can it be said that each glucose ...

Q. Why can it be said that each glucose molecule runs the Krebs cycle twice? Each glucose molecule "cycles" the Krebs cycle twice because after glycolysis each used glucose has

Explain free water - water found in food, Explain Free Water - Water Found ...

Explain Free Water - Water Found in Food? Water which is bound by such minute forces, that its vapour pressure is equal to the vapour pressure of pure water. It can be found as

State the term- hue, State the term- Hue Hue  is the dominant spectral ...

State the term- Hue Hue  is the dominant spectral colour (rainbow) and is related to the wavelength of light. In Munsell system there are five principal hues; red (R), yellow (

Orthopedics, 1. List alternative therapies for musculoskeletal problems. ...

1. List alternative therapies for musculoskeletal problems. 2. What is a xyrospasm? What is the Greek derivative of the word? 3. Name and describe the three types of varus con

Project for seminar, Ask question #Minimum 100 words acceptethe best topics...

Ask question #Minimum 100 words acceptethe best topics for seminAR d#

Left atrio-ventricular, The left atrio-ventricular aperture in man is guard...

The left atrio-ventricular aperture in man is guarded by

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd