How much template dna will you add, Biology

Assignment Help:

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?


Related Discussions:- How much template dna will you add

What is the spontaneous generation hypothesis, What is the spontaneous gene...

What is the spontaneous generation hypothesis? The impulsive generation abiogenesis or hypothesys asserts that life on earth has come from nonliving material. For instance, t

Hypothalamus and pituitary, Hypothalamus and Pituitary The most obviou...

Hypothalamus and Pituitary The most obvious neuroendocrine link is between the hypothalamus and pituitary. The hypothalamus is a part of the brain which is connected to the pi

Describe how the various molecular mechanisms act, 1. Using specific exampl...

1. Using specific examples describe how variations in DNA sequence between individuals can lead to risk of disease. Describe how a range of techniques have been adapted to detect s

Study of human age pyramids, Q. What are the analyses provided by the study...

Q. What are the analyses provided by the study of human age pyramids? The study of human age pyramids able to provide the following analyses, proportion of individuals at an ec

What is the endocrine function of the placenta, Q. What is the endocrine fu...

Q. What is the endocrine function of the placenta? The placenta besides being the organ through which the exchange of substances between the fetus and the mother is done also h

Diversity, who is the farther of bio diversity?

who is the farther of bio diversity?

Electron transport chain, ELECTRON TRANSPORT CHAIN All processes requir...

ELECTRON TRANSPORT CHAIN All processes require energy. In living cells, we constantly use energy for a number of biochemical reactions e.g. muscular movements, synthesis  of ne

What is an instance of a hypothesis, Q. What is an instance of a hypothesis...

Q. What is an instance of a hypothesis which may explicate why there is not a big representation of the class Reptilia found in Polar Regions? Beings of the class Reptilia are

What is the name of group of fungi, Most of the fungi normally observed are...

Most of the fungi normally observed are members of this group of fungi that includes toadstools, mushrooms and puffballs. This group of fungi is termed as: a) Phycomycetes (pro

Congenital discrete subvalver stenosis, Congenital Discrete Subvalver Steno...

Congenital Discrete Subvalver Stenosis : The abstraction could be fibrous or fibro muscular. It could be anywhere from just below the aortic cusps upto the level of the free edge

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd