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Q. How execute the two complementary nucleotide chains of the DNA facilitate the replication process of the molecule?
The fact that DNA molecule is made of two polynucleotide chains whose nitrogen-containing bases form hydrogen bonds facilitates the duplication of the molecule. During the DNA replication, the binding of the two chains is broken and each of them serves as a template for the formation of a new nucleotide sequence along it, with the help of the enzyme the DNA polymerase and obeying the pairing rule C-G, A-T. At the end of the process two double helix of DNA are produced, all made of an original template chain and of a new synthesized polynucleotide chain.
Assume your tilapia fish muscle homogenate has a protein concentration of 5769 ?g/ml. You take 32.5 ?l of this fish homogenate, mix it with 12.5 ?l sample buffer and 5 ?l reducing
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