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Q. How can amine groups be classified?
Amines can be classified into primary amines, those to which one -R (variable radical) is attached to a -NH2, secondary amines, those where one hydrogen of NH2 is substituted by another -R, thus having two -R, and tertiary amines, those with no hydrogen bound to the nitrogen and with three - R.
Assume that you have 1 ml of a solution of amylase (an enzyme) at a concentration of 15 mg protein/ml. Calculate the volume of diluting buffer that you would have to add to 1.0 ml
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