Green fluorescent protein - mitochondrial inner membrane, Biology

Assignment Help:

You are studying how proteins are targeted to the mitochondria. MIP1 is in the mitochondrial inner membrane, whereas MIP2 is targeted to the matrix. You are most interested in how proteins get into the inner membrane, so you focus on MIP1.

A) Analysis of a GFP-tagged MIP1 in a Tom 20 yeast mutant suggests that MIP1 targeting is Tom 20-dependent. Where would the GFP-tagged MIP1 be found in this mutant? Include your rationale in your answer.

B) Another scientist has already identified the matrix-targeting sequence at the N-terminus of MIP1. You think you have also found a stop-transfer sequence. How would you determine if the sequence you identified is NECESSARY for targeting to the membrane? You have the following tools (and you don't have to use all of them):

-GFP-tagged MIP1

-GFP-tagged MIP1 with the putative stop-transfer sequence mutated

-GFP-tagged MIP2

-GFP-tagged MIP2 with the putative stop-transfer sequence added

-Yeast

Describe the experiment including controls. State your predicted results if the sequence is necessary for inner membrane targeting.


Related Discussions:- Green fluorescent protein - mitochondrial inner membrane

Self quiz, levels of biological organization in order from smallest to larg...

levels of biological organization in order from smallest to largest

Excretion in cockroach, EXCRETIO N IN COCKROACH- Animal is uricotelic....

EXCRETIO N IN COCKROACH- Animal is uricotelic. Excretory organs are malphighian tubules. 60-150 in 6-8 groups. In each group 10-15. Yellow coloured, blind tube, open in gut

State the uni-ocular movements, State the Uni-ocular Movements In each...

State the Uni-ocular Movements In each eye, for every movement there is an agonist, antagonist, and a synergist. An agonist is the main muscle that is active in carrying out t

How parasitic infections are found, How Parasitic infections are found? ...

How Parasitic infections are found? Parasitic infections are found throughout the world. With increasing travel, immigration, use of immunosuppressive drugs and the spread of A

Mold, Does mold have roots? When mold is inside on wood - are there roots t...

Does mold have roots? When mold is inside on wood - are there roots that go into substrate? I am trying to find this out today if possible to evaluate a remediator''s plan. I am wo

What do you understand by flagella, What do you understand by Flagella. ...

What do you understand by Flagella. A cellular hairlike locomotory structure which comprise an extension of the plasma membrane surrounding a 9+2 organization of microtubules.

Explain leptospirosis, Leptospirosis It is a bacterial disease that oc...

Leptospirosis It is a bacterial disease that occurs in many domestic and wild animals, is endemic worldwide, but the highest incidence is in tropical and subtropical areas. Tr

Define glass photo-emissive tubes, Define Glass photo-emissive tubes? G...

Define Glass photo-emissive tubes? Glass photoemissive tubes are those which consist of a curved metal cathode, coated with a photosensitive material. While light strikes its s

Fungal diseases - aspergillosis, F u n g a l diseases A spergill...

F u n g a l diseases A spergillosis Aspergillosis is mycotic disease of poultry and all other species of birds caused by a fungus known as Aspergillus fumigatus . F

Explain rodent control and pests, Q. Explain Rodent Control and pests? ...

Q. Explain Rodent Control and pests? In any food establishment, varieties of insects, pests and rodents pose a big threat to the maintenance of hygienic surroundings. They

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd