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The movement of Na+ and glucose from the lumen of the intestine across the epithelial cell to the blood sets up a dissimilarity in osmotic pressure across the cell. As per the result, water flows by the cell, across the apical and basolateral membranes by easy diffusion. Therefore the uptake of water needs both Na+ and glucose (or amino acids) to be present in the lumen of the intestine. The incidence of water alone in the lumen of the intestine is much fewer effective. That is the basis of glucose rehydration therapy as a remedy for dehydration; a solution of glucose and salt (NaCl) is administered to the patient. This is a easy inexpensive but very extremely significant treatment that has saved the lives of a lot of infants in developing countries who would have otherwise died of the effects of dehydration, commonly associated with diarrhea.
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What technique would you use to see if two proteins interact with each other inside of a cell?
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