Genomic dna libraries , Biology

Assignment Help:

A genomic DNA library is made from the genomic DNA of an organism. For instance, a mouse genomic library could be made by digesting mouse nuclear DNA with a restriction nuclease to make a huge number of several DNA fragments but all with identical cohesive ends.

175_22.png

           Figure:  A simple method of DNA cloning using a plasmid vector

The DNA fragments would then be ligated into linearized plasmid vector molecules or into an appropriate virus vector.  This library would hold all of the nuclear DNA sequence of the mouse and could be searched for any ceratin mouse gene of interest.  Each clone in the library is called as a genomic DNA clone. Not each genomic DNA clone would hold a complete gene since in various cases the restriction enzyme will have cut at least once within the gene. Thus some clones will have only a category of a gene.

 


Related Discussions:- Genomic dna libraries

Embryonic stem cells (es), E mb ryonic stem cells (ES): These a...

E mb ryonic stem cells (ES): These are pleuripotent cells of early embryo (blastocyst) capable of giving rise to all differentiated cells, including germ line cells. Em

Multiple membrane-spanning proteins, Various integral proteins have multipl...

Various integral proteins have multiple membrane-spanning   -helices. Bacteriorhodopsin, a protein found in a photosynthetic bacterium captures the energy from light and uses it to

Define volumetric flasks - nutritional biochemistry, Define volumetric flas...

Define volumetric flasks - Nutritional Biochemistry A volumetric flask is used to make up a solution of fixed volume very accurately. It is calibrated to contain a particular v

Conductometry, Highlight ten applications of conductometry and explain four...

Highlight ten applications of conductometry and explain four in detail

What is the results of congenital pulmonary stenosis, What is the Results o...

What is the Results of Congenital Pulmonary Stenosis? Early mortality for open pulmonary valvotomy in neonates varies between 6 and 10 per cent. For infants and children, surgi

What are the abnormalities of gaze, What are the Abnormalities of gaze ...

What are the Abnormalities of gaze Normal gaze is when visual axes both eyes are parallel in primary gaze. when visual axes are not parallel in primary gaze, it is abnormal ga

Define absorption, Define Absorption, transport and Excretion of phosphorus...

Define Absorption, transport and Excretion of phosphorus? As you have seen that food contains both organic and inorganic phosphorus, but most of it is absorbed in its inorganic

Explain coiling of garden pea tendrils, Coiling of garden pea tendrils arou...

Coiling of garden pea tendrils around any support is an example of: 1. Thigmotaxis 2. Thigmonasty 3. Thigmotropism 4. Thermotaxis Thigmotropism

Haemorrhagic septicaemia, H a e m o r r ha g i c septicaemia ...

H a e m o r r ha g i c septicaemia It is also known as septicemic pasteurellosis or barbone and the disease is clinically characterized by high fever, excessive saliv

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd