Genomic dna fragments, Biology

Assignment Help:

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 


Related Discussions:- Genomic dna fragments

Biogeochemical cycle, The cyclic exchange of nutrient material between orga...

The cyclic exchange of nutrient material between organisms and non-living  environment is called biogeochemical  cycle ,'geo means earth: ''chemical' means chemical elements, thus

PHYLUM PROTOZOA, STATE THE ECONOMIC AND ECOLOGICAL IMPORTANCE OF ECHINODERM...

STATE THE ECONOMIC AND ECOLOGICAL IMPORTANCE OF ECHINODERMS

Why are salt and sugar used in the production of dried meat, Why are salt a...

Why are salt and sugar used in the production of dried meat and dried fruits? Substances that maintain a highly hypertonic environment, such as sugar and salt, are used in the

#title.growth hormone., according to the graph,which age is the amount og g...

according to the graph,which age is the amount og growth hormone produced at its highest?

State the term - migraine stroke, State the term - Migraine Stroke Sinc...

State the term - Migraine Stroke Since the late 1800s, physicians have recognised that migraine attacks may lead to infarcts and permanent neurological deficits. Such migraine

Marine extremophiles, how to make a assignment on recent research on marine...

how to make a assignment on recent research on marine microbiology

Does the fish heart pump venous or arterial blood, Q. Does the fish heart p...

Q. Does the fish heart pump venous or arterial blood? After oxygenation in the gills the arterial blood goes to the tissues so the fish heart pumps venous blood, the venous blo

Explain the nutritional and functional role of zinc, Minerals  :-  Zinc ...

Minerals  :-  Zinc Food Source      Meats, cereals.  Nutritional Functional role Essential nutrient: Deficiency produces loss of appetite, growth retardation, skin

Categorisation of major brain functions, Categorisation of Major Brain Func...

Categorisation of Major Brain Functions As a framework, the major brain functions typically divided into modalities and domains. The major modalities are motor function and th

Explain the basic concept of proteins, Explain the Basic Concept of Protein...

Explain the Basic Concept of Proteins? Proteins, as we already knows by now are products of amino acids. Each molecule of protein is composed of many molecules of amino acids j

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd