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You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.
If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.
Explain the Impedance method to Measure Body Fats? Impedance method: This method can give entire body composition like total body water (TBW), fat free mass (FFM) or lean body
Adverse Effects of Imiquimod Application site reactions (irritation, pruritus, flaking, erosion) are generally mild to moderate in intensity and resolve within 2 weeks of cessa
South Dakota and Oregon are at similar latitudes, and yet, on average, Oregon has warmer winters and cooler summers than South Dakota does. What property of water helps account for
When the level of AMP is high, this shows the requirement for more ATP synthesis. AMP stimulates PFK increases the rate of glycolysis and inhibits fructose 1 and 6- bisphosphatase
Explain the process of Absorption of Fats? After digestion, only 25% of triglycerides are broken completely to glycerol and fatty acids. Major digestion product is 2-monoacylgl
What is the Determination of vitamin B 2 In the chemical method, the yellow-green fluorescence of aqueous solution of riboflavin is measured. In the microbiological assay, tur
Symptoms contain fever, weakness, rashes, with erythema and edema. Serum sickness depends on the immune difficult formed and the size of the complexes.
Wat type of animal is tick and weevil?
give detail account of modes of locomotion in protozoa and describe various type of asexual reproduction in protozoa
Phases of Seed Formation The process of seed formation can be divided into two phases: The division phase, in which fertilised egg divides mitotically and complet
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