Genomic dna fragments, Biology

Assignment Help:

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 


Related Discussions:- Genomic dna fragments

Learning objectives of coronary artery disease, Q. Learning objectives of c...

Q. Learning objectives of coronary artery disease? • describe the various forms of coronary heart diseases, • enumerate the risk Factors (genetic and environmental) in causati

Describe surgical treatment prosthetic valve endocarditis, Describe Surgica...

Describe Surgical Treatment prosthetic valve endocarditis ? If there is definite indication for early surgery, the current understanding is to proceed with valve replacement ir

List disbeliefs which patients have about insulin, Q. List disbeliefs which...

Q. List disbeliefs which patients have about insulin? a) Insulin is not effective. Some patients believe that insulin is not effective for treating diabetes. b) Insulin c

Packaging of meat products, P a ck a g i n g of Meat Products Me...

P a ck a g i n g of Meat Products Meat needs to be packaged to prevent contamination, color deterioration, loss of moisture, odor pickup, oxidative rancidity etc and pro

Explain the dark adaptation of vitamin a, Explain the Dark Adaptation of Vi...

Explain the Dark Adaptation of Vitamin A? In the early stages of VAD, the individual cannot see objects in dim light. This' phenomenon is used as a criterion for assessment in

How sequences of events occur to activate protein kinase a, When glucagon b...

When glucagon binds to it's receptor on a liver cell, which one of the following sequences of events occurs to activate protein kinase A? -activation of G protein, activation of

What are the functions of the eyelids, What are the functions of the Eyelid...

What are the functions of the Eyelids? Functions of the Eyelids: The eyelid makes the outer most layers, covering the eye. Because of its strategic position, the eyelid s

Animal around us, #question.what are the reason that arthropodans are abund...

#question.what are the reason that arthropodans are abundant in nature.

In which part of the digestive tube is water is absorbed, Q. In which part ...

Q. In which part of the digestive tube is water is chiefly absorbed? What about the mineral ions and vitamins? Most part of water, mineral and vitamins ions are absorbed by the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd