Genomic dna fragments, Biology

Assignment Help:

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 


Related Discussions:- Genomic dna fragments

Nitrogen fixation, Nitrogen Fixation Nitrogen is an essential constitue...

Nitrogen Fixation Nitrogen is an essential constituent of living organisms and there is an inexhaustible supply of it in the atmosphere in the free form. Majority of living o

What is a plant-incorporated protectant, What is a plant-incorporated prote...

What is a plant-incorporated protectant? Plant-incorporated protectants are pesticidal substances formed and used by the living plant, typically to protect the plant from pests

Pre-embryo - human development, Pre-Embryo - Human Development About t...

Pre-Embryo - Human Development About thirty hours after fertilization the zygote goes through mitosis giving rise to 2 blastomeres. These blastomeres go through further cleava

Define results of miscellaneous conditions, Results :  In the present day,...

Results :  In the present day, surgical mortality with or without CABG is repture to be 5-7 per cent. The late survival is 85 per cent, 75 per cent and 65 per cent at 1, 3 and 5

Active Transport, How does the addition of a watery secretion to the conten...

How does the addition of a watery secretion to the contents of the gut help in the process of digestion?

Benefits of cross-pollination, Benefits of Cross-Pollination Because o...

Benefits of Cross-Pollination Because of the specific benefits of cross-pollination, flowering plants have evolved many devices to prevent self-pollination and to encourage cr

Closed style - style of stigma interaction, Closed Style - Style of Stigma ...

Closed Style - Style of Stigma Interaction Cotton shows an epidermis with stomata, a cortex of thin-walled parenchyma with several vascular bundles and strands of transmitting

Heroin (diacetylmorphine or diamorphine), HEROI N (DIACETYLMORPHINE OR DIA...

HEROI N (DIACETYLMORPHINE OR DIAMORPHINE) - Heroin is a white, crystalline semi synthetic compound prepared from morphine by acetylation. Most dangerous opiate. It i

What does boiling do to an enzyme, What does boiling do to an enzyme? Enz...

What does boiling do to an enzyme? Enzymes are denatured (their shape is changed so it doesn't work) when boiled.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd