Genomic dna fragments, Biology

Assignment Help:

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 


Related Discussions:- Genomic dna fragments

Structure of spermatozoan, STRUCTUR E OF SPERMATOZOAN Structure of spe...

STRUCTUR E OF SPERMATOZOAN Structure of sperm is divided into 3 parts -      (1) Head                  (2) Middle piece             (3) Tail 1 .       HEAD

Define obligatory and facultative water excretion, Define Obligatory and Fa...

Define Obligatory and Facultative water excretion? i) Obligatory water excretion: The kidney is 'obligated' to excrete some water to rid the body of its daily load of urinary s

Determine the stabilized forms of natural colourants, Stabilized forms of n...

Stabilized forms of natural colourants The mimicking of the native environment in which a natural colour exists is just one way of producing a stabilized form. In this case, th

Explain why statement is logical in terms of kin selection, Biology once sa...

Biology once said why this statement is logical in terms of kin selection and use the coefficient of relatedness he'd be willing to lay down his life to save two brothers or eight

Describe dna replication in details, Describe DNA replication in details? ...

Describe DNA replication in details? Replication :  DNA replicates itself by first breaking the hydrogen bonds between the nitrogen base pairs, and "unzips" itself into two s

Phylum arthropoda - classification of multicellular animals, Phylum Arthrop...

Phylum Arthropoda - Classification of Multicellular Animals Earlier you known how the coelom evolved and the body of the coelomates got segmented leading to evolution of segme

When aflatoxigenic molds can occur, Q. When Aflatoxigenic molds can occur? ...

Q. When Aflatoxigenic molds can occur? Occurrence: Aflatoxigenic molds can occur in warmer parts of the world and aflatoxicosis maybe produced in a wide range of tropical and s

Steps of fatty acid synthesis, Fatty acids are synthesized by the condensat...

Fatty acids are synthesized by the condensation of two-carbon units. Furthermore, in  terms  of  the  enzymic  steps  contained,  the  procedure  is  not  the  reverse  of  β- oxid

Results of pericardial diseases, Results :  Pericardiectomy used to have a...

Results :  Pericardiectomy used to have a mortality of 10-15 per cent in the earlier era. At present it is around 3 to 5 per cent and does not approach 0 per cent even though it

Why is the proximity between ribosomes and amino acids, Why is the proximit...

Why is the proximity between ribosomes and amino acids important for the protein formation? What is the enzyme that catalyzes that reaction? The proximity among ribosomes and a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd