Genomic dna fragments, Biology

Assignment Help:

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 


Related Discussions:- Genomic dna fragments

Define osseointegration and its theories, Q. Define osseointegration and it...

Q. Define osseointegration and its theories? Osseointegration implies that "it is a contact established without interposition of non bony tissue between normal remodeled bone a

Define regulation of water balance - for digestive process, Define Regulati...

Define Regulation of Water Balance - For the Digestive Process? For the digestive process alone, the estimated daily volume of fluid that enters and leaves the gastrointestinal

Ripening bananas, you tested three different treatments for ripening banana...

you tested three different treatments for ripening bananas. Before you did the lab, which one did you think would have ripened most? State your hypothesis and explain your reason

Define signs and symptoms of iron deficiency anaemia, Define Signs and symp...

Define Signs and symptoms of iron deficiency anaemia? Since the level of haemoglobin is reduced in the blood, it causes paleness (pallor) on certain parts of the body. Initiall

What is the role of linked traits in genetics, What is the role of Linked T...

What is the role of Linked Traits in genetics? In his study of inheritance in the fruit fly, Morgan observed that certain genes are distributed together, or linked, rather than

What are the biological troubles, Q What are the biological troubles that m...

Q What are the biological troubles that molluscs face due to their soft body? Because molluscs have a soft body they are further fragile. They also have more complexity to supp

Define requirements of sodium during pregnancy period, Define requirements ...

Define requirements of Sodium during pregnancy period? As you know, sodium plays a role in fluid balance. Sodium metabolism is altered during pregnancy under the stimulus of a

Illustrate the diabetic contract of the eye, Illustrate the diabetic contra...

Illustrate the diabetic contract of the eye? Diabetic Cataract: 1) Sorbitol Aldose Pathway: In patients with diabetes, there is increase in sugar alcohol which results in

Influence of diabetes mellitus, Influence of Diabetes Mellitus :  Diabetes...

Influence of Diabetes Mellitus :  Diabetes causes micro vascular obstructive disease in heart, kidney, retina and peripheral nerves. Scrupulous rather than casual control of diabe

Steps to determine thermal resistance of microorganism, Define Steps To det...

Define Steps To determine thermal resistance of a microorganism? Most food components and microorganisms obey first order reaction kinetics, which means that the destruction ra

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd