Genomic dna fragments, Biology

Assignment Help:

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 


Related Discussions:- Genomic dna fragments

Determine the enzymes utilization in food industry, Enzymes utilization in ...

Enzymes utilization in food industry  Enzymes may be used in industry as components of living cells or after isolation in free or immobilized forms. All of them may be referred

TISSUES, difference between axon and cyton

difference between axon and cyton

Hydrosphere, The water on earth surface constitutes hydrosphere. About 75% ...

The water on earth surface constitutes hydrosphere. About 75% of earth surface is covered with water and 97% of that water is present in oceans, which is very salty. We are only

Describe the various types of rna, Describe the various types of RNA? ...

Describe the various types of RNA? Types of RNA :  There are three different types of RNA, all formed by transcription from DNA: (1) Messenger RNA (mRNA) is a single unc

Intrisic and Extrinsic Limiting Factors, How do the ideas of energy and che...

How do the ideas of energy and chemical cycles, community structure, biodiversity and succession fit together to form the basis of the way the natural world works?

Algae, The difference b/n algae,bryophyte&pteridophyte?

The difference b/n algae,bryophyte&pteridophyte?

Genetics, what about cytoplasmic sex determination

what about cytoplasmic sex determination

Post-operative care of teeth, Post-operative care of teeth After implan...

Post-operative care of teeth After implant surgery, patients should be warned to expect:  Some swelling and possibly bruising.  Some discomfort which can usually be contr

Explain wind pollinated flowers, Wind pollinated flowers are: 1. Small, ...

Wind pollinated flowers are: 1. Small, brightly coloured, producing large number of pollen grains 2. Small, producing large number of dry pollen grains 3. Large producing

Taxonomy, newer trends of animal taxonomy

newer trends of animal taxonomy

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd