Genomic dna fragments, Biology

Assignment Help:

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 


Related Discussions:- Genomic dna fragments

Categorisation of major brain functions, Categorisation of Major Brain Func...

Categorisation of Major Brain Functions As a framework, the major brain functions typically divided into modalities and domains. The major modalities are motor function and th

Biodiversity maintains, Biodiversity maintains the air we breathe and the w...

Biodiversity maintains the air we breathe and the water we drink. Green plants purify our air and our water by taking in carbon dioxide, regulating water vapour, releasing oxygen,

Pulmonary valvotomy cardio bypass and use cardioplegia, Pulmonary Valvo...

Pulmonary Valvotomy on Cardio Pulmonary Bypass and use of Cardioplegia :  Single aortic cannulation and separate SVC and IVC cannulation arc done. The cavae are snared to

What do you mean by dicoumarol, Q. What is dicoumarol? How does this substa...

Q. What is dicoumarol? How does this substance act in the clotting process and what are some examples of its toxicity? Coumarinic anticoagulants cannot be administered during p

Define precaution-estimation of nitrogen and protein content, Define Precau...

Define Precautions - Estimation of Nitrogen and Protein Content? 1. The apparatus should be airtight to prevent leakage of NH 3 . 2. The tip of the delivery tube should be i

Define evolution of food processing, Define Evolution of food processing? ...

Define Evolution of food processing? 8000 - 7000 BC. Mankind first began farming, growing crops and raising animals for food instead of hunting and gathering for food.

How can total count can be obtained?, How Can Total Count Can Be Obtained? ...

How Can Total Count Can Be Obtained? Total count can be obtained microscopically by using fluorescent dyes such as acridine orange or DNA stain DAPI. The counts obtained micros

Rules followed by diabetic patient, Q Show Rules followed by diabetic patie...

Q Show Rules followed by diabetic patients? A  "sick day" is any health problem diabetic patient may have. This could include a common cold, infection, diarrhoea, fever, injury

What are neurons, Question 1 Give the definition and describe the mechanis...

Question 1 Give the definition and describe the mechanism of Active transport. Passive transport with suitable examples Question 2 What are neurons? List out the

Define classification of carbohydrates - oligosaccharides, Define classific...

Define classification of carbohydrates - Oligosaccharides? Oligosaccharides are short chains of saccharide units and are condensation products of three to ten monosaccharides.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd