Genomic dna fragments, Biology

Assignment Help:

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 


Related Discussions:- Genomic dna fragments

Dissolved gases and alkalinity, Dissolved Gases and Alkalinity Disso...

Dissolved Gases and Alkalinity Dissolved Gases The marine environment serves as a gigantic reservoir of dissolved oxygen and carbon dioxide, which respectively help reg

Bacterial diseases- black quarter, Black quarter Synonym: BlacklegBlack...

Black quarter Synonym: BlacklegBlack-quarter (BQ) is an acute infection but a non-contagious disease characterized by inflammation of skeletal and cardiac muscles, severe toxae

Types of osmotic exchanges, Types of Osmotic Exchanges The osmotic exc...

Types of Osmotic Exchanges The osmotic exchanges that take place between an animal and its environment are of two different types: Obligatory exchanges and Regulated

What are neurons, Question 1 Give the definition and describe the mechanis...

Question 1 Give the definition and describe the mechanism of Active transport. Passive transport with suitable examples Question 2 What are neurons? List out the

What do you mean by tympanum, Q. What is the tympanum? In which part of the...

Q. What is the tympanum? In which part of the ear is it located and what is its function? The tympanum (or ear drum) is a membrane located in the middle ear just after the audi

Is heart patient can do exercise or not, Is heart patient can do exercise ...

Is heart patient can do exercise Advise the patient to consult the doctor before starting any exercise. Doctor will advise the patient to choose exercises as per his condition;

Avian leukosis (sarcoma group of retroviruses), A v i a n leukosis (Sar...

A v i a n leukosis (Sarcoma group of retroviruses) This is a complex of viral diseases caused by an avian retrovirus with various manifestations such as lymphoid leukosis,

Post-mating isolating mechanisms, The second category of isolating mechanis...

The second category of isolating mechanism that may permit interspecific mating but ensures reduced viability is called post-mating isolating mechanism. This category could be subd

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd