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You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.
If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.
Q. Explain Barrier techniques - Protective clothing? Protective outerwear is worn to reduce exposure to debris in spatter. Clinic jackets should have long sleeves, a high neck,
PEROXISOMES AND GLYOXYSOMES Certain small ovoid unit membrane bound sac like cytoplasmic organelles, resembling lysosomes in size and appearance were isolated from var
Hepaticae (Liverworts) The gametophyte is thalloid body or simple stem and leaves, with aquatic or semi-terrestrial habit. Rhizoids are multiceillular. Some examples : Mar
This process involves the regulation of the concentration of the body fluids through control of water content and salt content of the blood. If a person loses water from their bloo
Metastasis - Characteristics Define Cancer Metastasis is the capability of a malignant cell to detach itself from a tumor and establish a tumor in another site. This capabilit
Why is the AIDS treatment often done with a drug cocktail? The treatment of acquired immune deficiency syndrome is often done with one or more anti-retroviral drugs of dissimil
1. In modern blimps, the gas of choice used to inflate them is helium rather than hydrogen. Hydrogen would be lighter, but helium is safer. Compare and contrast the atomic structur
Determine about benefits of Jogging Jogging gives a sense of wellness to a diabetic patient. The frequency of jogging should be at least 3 days in week with a distance of 2 km
Explain function of lymph nodes? Lymph nodes have white blood cells which ingest bacteria and prevent them from reaching the circulation.
It is important to explain to the patient about importance of taking treatment regularly. In case he misses the dose or takes medicine or insulin irregularly, explain the side effe
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