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You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.
If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.
Food Chain - Ecosystem In a food chain, the food energy is transformed from a given source through a series of species, each of which eats the one before itself in the chain.
You are studying a gene, and its effects on skin cells. You want to find out A) If the protein made encoded by this gene binds to DNA in a particular place B) if the protein binds
A 20 year old female university student came to the clinic complaining of palpitations. She has had occasional rapid heart rates but never lasting more than 2 minutes. This time it
Explain what is Third Heart Sound S 3 ? S, is generated during the rapid filling pliase. It is a low frequency event. Mechanism of S, production: It is due to a sudden limita
Q. What are the three major types of passive transport? The three main types of passive transport are simple osmosis, diffusion and facilitated diffusion.
Micrographia is abnormally small, cramped handwriting and/or the progression to continually smaller handwriting. This is one of the symptoms of Parkinson''s disease.
For patients with end-stage heart failure, cardiac transplantation has become a promising therapy especially with the advent of immunosuppressive therapy and more careful screenin
Explain acylglycerols The most abundant class of food lipids is the acylglycerols, also known as glycerol esters of fatty acids, which dominate the composition of depot fats in
Define the Disturbances in Fluid Balance? The correct functioning of cells and tissues depends on appropriate concentrations of nutrients; so any abnormal loss or accumulation
Q. What is the name of the molecule that transports oxygen in red blood cells? The respiratory pigment of the red blood cells is hemoglobin.
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