Genomic dna fragments, Biology

Assignment Help:

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 


Related Discussions:- Genomic dna fragments

Are there aquatic and flying mammals, Q. Are there aquatic and flying mamma...

Q. Are there aquatic and flying mammals? Sirenians (dugongs, manatees) and Cetaceans (whales, dolphins) are aquatic mammals Chiropterans (bats) are flying mammals.

Explain procedure for gram staining of bacterial cultures, Explain Procedur...

Explain Procedure for Gram Staining of Bacterial Cultures? Now carry out the exercise following the steps enumerated herewith. 1. Prepare bacterial smear on a clean, non-gre

Define peri-implantitis, What is peri-implantitis? Peri-implantitis as ...

What is peri-implantitis? Peri-implantitis as defined by Meffert is the progressive loss of peri-implant bone as well as soft tissue inflammatory changes. Bacterial invasion of

Bacteria, Bacteria The study of bacteria is called bacteriology. Bacte...

Bacteria The study of bacteria is called bacteriology. Bacteria are unicellular organisms and possess a distinct cell wall. They are included in kingdom Prokaryote. The bacter

Poultry and duck diseases-infectious laryngotracheitis (ilt), Infectious la...

Infectious laryngotracheitis (ILT) Infectious laryngotracheities, caused by fowl Gallid Herpesvirus 1 belonging to the family Herpesviridae, is a quickly spreading acute res

Determine the carbon based materials, Carbon Based Materials These incl...

Carbon Based Materials These include Pyrolytic carbon, polycrystalline (Vitreous Carbon), and carbon/silicon interstitial combination. Vitreous Carbon -Solid or porous vitre

Activitiesthat cause fish kills, Explain how human activities can cause an ...

Explain how human activities can cause an imbalance in biogeochemical cycling and lead to problems such as cultural eutrophication and fish kills

How do organisms adjust to changes in temperature, How do organisms adjust ...

How do organisms adjust to changes in temperature? Some of the most ordinary way for an organism to adjust to changes in body temperature is by perspiration or panting.

Explain how inhibition might contribute, We now understand that mutations t...

We now understand that mutations that cause the inhibition of apoptosis are found in tumors. Because proliferation itself is not induced by the inhibition of apoptosis, explain how

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd