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You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.
If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.
translation in eukaryotes..
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Didanosine (ddl, Videx) Didanosine is available as buffered tablets, buffered or non-buffered powder, and non-buffered enteric-coated capsules (Videx EC). Adverse effects
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A haploid cell contains: A.one half of a complete set of chromosomes B.several complete sets of chromosomes C.the correct number of chromosomes D.two complete sets of chromosomes E
Properties of Phytochrome Phytochrome is a chromoprotein: this means it is composed of a protein and a chromophore. The chromophore is actually attached to the protein and is
Compare and contrast the effect of a deletion in the operator of the lactose operon with one in the operator of the histidine operon.
This constitutes about 2.4% of the body weight of man viz. Brain-1400 gram Spinal nerve-150 gram Spinal cord-30 gram Cranial nerve-10 gram C.S.F. is altogether not present in
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