Genomic dna fragments, Biology

Assignment Help:

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 


Related Discussions:- Genomic dna fragments

Effect of a deletion in the operator of the lactose operon, Compare and con...

Compare and contrast the effect of a deletion in the operator of the lactose operon with one in the operator of the histidine operon.

Respiration in insect - cockroach, RESPIR A TIO N IN INSECT (COCKROACH) ...

RESPIR A TIO N IN INSECT (COCKROACH) - Respiration is direct. So metabolic rate is high. This system is related to each cell of the body so respiratory pigment is ab

Pethidine and methadone, PETHIDIN E - Meperidine hydrochloride. ...

PETHIDIN E - Meperidine hydrochloride. Widely used as narcotic analgesic. It has local anesthetic action. METHADON E - It is an orally effective anal

Explain about the biofertilizers, Explain about the Biofertilizers Biof...

Explain about the Biofertilizers Biofertilizers, are the cultures of microorganisms used for inoculating seed or soil or both under ideal conditions to increase the availabilit

Respiratory system - developmental changes, Respiratory System - Developmen...

Respiratory System - Developmental Changes after Birth The new-born's lungs are collapsed and need powerful breaths to inflate them; airways too are small, offering remarkable

How are poriferans characterized, Q. Concerning digestion how are poriferan...

Q. Concerning digestion how are poriferans characterized? Sponges are diverse from other animals since they present only intracellular digestion. Do they release digestive enzy

Ciguatera fish poisoning, Q. Explain Ciguatera Fish Poisoning? Ciguater...

Q. Explain Ciguatera Fish Poisoning? Ciguatera probably represents the most common cause of poisoning from fish in tropics. Ciguatera comprises over 50% of all reported case

Fowl typhoid, F o wl typhoid Fowl typhoid is caused by Salmonella G...

F o wl typhoid Fowl typhoid is caused by Salmonella Gallinarum , a Gram negative bacillus in the family enterobacteriaceae (serogroup D). Chickens are the natural hosts fo

Fate of pyruvate, Pyruvate Pyruvate has three different  fates. Under a...

Pyruvate Pyruvate has three different  fates. Under aerobic conditions,  pyruvate enters mitochondriaand is converted to acetyl CoA. The acetyl CoA enters thecitric acid cycle.

Vaccines, Ask quesWhat type of antigenic agents may constitute vaccines? ti...

Ask quesWhat type of antigenic agents may constitute vaccines? tion #Minimum 100 words accepted#

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd