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You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.
If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.
The cyclic exchange of nutrient material between organisms and non-living environment is called biogeochemical cycle ,'geo means earth: ''chemical' means chemical elements, thus
STATE THE ECONOMIC AND ECOLOGICAL IMPORTANCE OF ECHINODERMS
Why are salt and sugar used in the production of dried meat and dried fruits? Substances that maintain a highly hypertonic environment, such as sugar and salt, are used in the
according to the graph,which age is the amount og growth hormone produced at its highest?
State the term - Migraine Stroke Since the late 1800s, physicians have recognised that migraine attacks may lead to infarcts and permanent neurological deficits. Such migraine
how to make a assignment on recent research on marine microbiology
Q. Does the fish heart pump venous or arterial blood? After oxygenation in the gills the arterial blood goes to the tissues so the fish heart pumps venous blood, the venous blo
Minerals :- Zinc Food Source Meats, cereals. Nutritional Functional role Essential nutrient: Deficiency produces loss of appetite, growth retardation, skin
Categorisation of Major Brain Functions As a framework, the major brain functions typically divided into modalities and domains. The major modalities are motor function and th
Explain the Basic Concept of Proteins? Proteins, as we already knows by now are products of amino acids. Each molecule of protein is composed of many molecules of amino acids j
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