Genomic dna fragments, Biology

Assignment Help:

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 


Related Discussions:- Genomic dna fragments

What are the physical properties of tear film, What are the physical proper...

What are the physical properties of Tear Film? Physical Properties of Tear Film: The tear film is a three-layered structure as given below: 1) Outermost Layer: Lipid Layer

Show the collection of primary samples of grain, Q. Show the Collection of ...

Q. Show the Collection of Primary Samples of grain? Sampling of grains begins with the collection of the primary sample i.e. sample of the consignment. The process involved in

Ocean thermal energy, The oceans and absorb solar radiations. There exit a ...

The oceans and absorb solar radiations. There exit a temperature difference between surface and deep ocean water. Ocean thermal energy conversion plants convert the solar energy

Differentiate individual atoms true or false, What is the first step normal...

What is the first step normally taken when you look through the oculars? The nuclei of the cheek cells have been stained using a special dye so that they appear purple. What is the

Choices of microscope and stain, Name TWO choices of microscope and stain (...

Name TWO choices of microscope and stain (if stain should be used) that you could use to view each of the following. For example, to view human cheek cells you could use (1) bright

Diagram, a well lablled diagram of a tilapia

a well lablled diagram of a tilapia

What are the processes of the photochemical stage, What are the processes o...

What are the processes of the photochemical stage of the photosynthesis process? Photolysis of water, with liberation of molecular oxygen, and photophosphorylation of ADP, with

Digestive system - teeth, TEET H - Study of teeth is odontol...

TEET H - Study of teeth is odontology. Accumulation of oral bacteria & their products on teeth is plaque. Some acids are secreted by bacteria causing carries.

In which area lengthening of long bones occurs, Lengthening of long bones i...

Lengthening of long bones in humans takes place in a specific area of the bone. This area is known as the: a) Cancellous bone b) Medullary canal c) Periosteum (pron: per-

Causes of extinction, CAUSES OF EXTINCTION - 1 .       HUNTING - ...

CAUSES OF EXTINCTION - 1 .       HUNTING - Man started hunting wild animlas for food and safety trade and fun. It may be subsistence hunting, commercial hunting and sp

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd