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You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.
If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.
Q. What are the major events of the final mitotic period? The final mitotic phase is the telophase. In the telophase the following events occur decondensation of chromosomes ea
TRANQUILLIZER S - These are the drugs that reduce tension and anxiety, bring calmness without inducing sleep. (i) Phenothiazines - These are anti psychotic drugs.
Circulation, Respiration and Excretion of Asteroidea Asteroids depend upon the circulation of coelomic fluid for the transport of gases and some nutrients. The blood vascular
Q. Show Upsloping ST-Segment? There are few subjects in exercise literature that has generated more controversy than the significance of up sloping ST-segments. The up sloping
There is no Interphase preceding second meiotic division. There is a brief intervening period called Interkinesis During this period there may be synthesis of some reserve fo
Define about the Spectroscopy- colorimetry? Spectroscopy, therefore, is the study of the absorption and emission of radiation by matter. Absorption Spectroscopy is usually used
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Describe Gastrovascular cavity in detail? The name given to the internal cavity of cnidarians. A blind-ended (incomplete) gut with only one opening. Food is to be digested and
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