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You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.
If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.
What is the clitellum of earthtworms and where it is located? The clitellum is a special region of the annelid constituted by rings (metameres) with reproductive function. It c
Diffusion is the spreading of substance molecules from a region where the substance is more concentrated to other region where it is less concentrated. For example, during the boil
G L YCOLIPIDS They are lipids having sugar residues. Two common glycolipids are cerebrosides and gangliosides. (1) Cerebrosides They are made of amino alcohol sphi
Basic Research Terms: Some of the basic terms that are defined in this section are: Assumptions Operational Definitions Variables Delimitation and Limitation H
Exterrral and Internal Environment Let us try to understand the concept of environment with some examples. Can you identify the environment of a single carp fish in the pond? It
Please explain the differences between an integral membrane protein, a Davison-Danielli peripheral membrane protein, and a protein in which alpha-helices would not be found.
Hypoglycaemia When Driving Hypoglycemia is particularly dangerous if it happens to someone who is driving. People with hypoglycemia may have trouble concentrating or se
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What do you mean by Syconoid sponge. Of different sponge architectures, this is intermediate in its complexity. Spongocoel is no longer lined with choanocytes, now located in r
Once quite widespread throughout Asia and Africa, there has been a large reduction of the cheetah's habitat and numbers though it is still seen in widely separated areas of Africa.
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