Genomic dna fragments, Biology

Assignment Help:

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 


Related Discussions:- Genomic dna fragments

Polarity do water-soluble and fat-soluble substances, Q. Which type of pola...

Q. Which type of polarity do water-soluble and fat-soluble substances respectively have? Water-soluble substances are polar molecules that are they have electrically charged ar

Is the bacterium mrsa pathogenic or non-pathogenic, Is the bacterium MRSA p...

Is the bacterium MRSA pathogenic or non-pathogenic? MRSA bacteria are pathogenic. This group of bacteria belongs to Staphylococcus aureus family, which have grown resistant to

Gene targeting in somatic cells, Gene targeting in somatic cells: Since t...

Gene targeting in somatic cells: Since the pioneering work with sheep at Roslin cloning by nuclear transfer has now been exemplified in a number of other livestock species includ

Soil water-physical properties of soil, Soil Water Soil water relations...

Soil Water Soil water relationship is very important. In the fist place the supply of large quantities of water is necessary to satisfy the evapo-transpiration requirements of

Explain precautions for isolation of pure culture, Explain Precautions for ...

Explain Precautions for Isolation of Pure Culture? 1. Plating of the medium should be done 24 hours before performing the streaking. There should not be any moisture on the

What are nymph and imago, What are nymph and imago? Nymphs are larvae o...

What are nymph and imago? Nymphs are larvae of hemimetabolic insects (like grasshoppers). They are very same to the adult insect although smaller. In holometabolic insects (lik

Define the starch gel electrophoresis, Define the Starch gel electrophoresi...

Define the Starch gel electrophoresis? Starch gel has the advantage of fine texture and absence of protein adsorption with a molecular sieving effect retarding proteins of larg

Contraceptive mechanism of the iud, Q. What is the contraceptive mechanism ...

Q. What is the contraceptive mechanism of the IUD? The IUD (intrauterine device) is a piece of plastic coated with copper that is put-in within the uterus by a doctor. Coppe

What is the classification of burns, What is the Classification of Burns? ...

What is the Classification of Burns? Burns can be classified on the basis of the extent, depth, patient age and associated illness or injury. On the basis of depth, burns are u

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd