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Genomic blot is the type of Southern blot particularly used to analyze the mixture of DNA fragments derived from total genomic DNA. Because the genomic DNA is quite complicated, when it has been digested with the help of restriction enzymes, it produces a quite complex set of fragments ranging from tens of the bp to tens of the thousands of bp. Though, any specific gene will be reproducibly found on only one or a few particular fragments. A million identical cells will produce a million identical restriction fragments for any of the given gene, so probing a genomic Southern with a gene-specific probe will produce the pattern of perhaps one or of just a few bands.
Botulism Botulism is a rapidly fatal motor paralysis. It is caused by ingestion of toxin of Clostridium botulinum in food. Many species of domesticated, wild animals and birds
a) What is meant by 'vaso-dilation'? b) What are the effects of vaso-dilation in the skin? (a) Vaso-dilation is an enhance in diameter of small arterioles and
Define Renal Function & cardiovascular - change related with ageing? Changes associated with the cardiovascular and renal function: The progressive accumulation of athermanous
Define Disadvantages of using yeast as a source of protein? 1. Less protein yield (45-60%) 2. Growth rate is low (1-3 h) 3. High nucleic acid content leading to the forma
Width of Attached Gingiva at Implant Site The zone of attached gingiva needs to be intact and of adequate width to protect (by separating) the tissue around the implant. If it
Write short note on glycogen storage diseases. Glycogen storage diseases are caused by genetic defects that result in deficiencies in certain enzymes of glycogen metabolism.
Hazard Analysis Two steps recognized as preliminary and detailed analysis of hazard are taken. Preliminary Hazard Analysis (PHA) serves two aims: (i) It can expedite bringi
Describe the Methods of Assessment The four general principles outlined above, that is, adaptation, brain and behaviour, context, and development serve as the foundation for th
Q How does the amoeboid movement occur and what are examples of beings and cells that use such movements for locomotion? Amoeboid movements are created by cytoplasmic movements
What is Gene Expression ? Gene expression is the process by which a gene is activated to produce a certain protein. Cells are able to respond to changes in the environment i
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