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Genomic blot is the type of Southern blot particularly used to analyze the mixture of DNA fragments derived from total genomic DNA. Because the genomic DNA is quite complicated, when it has been digested with the help of restriction enzymes, it produces a quite complex set of fragments ranging from tens of the bp to tens of the thousands of bp. Though, any specific gene will be reproducibly found on only one or a few particular fragments. A million identical cells will produce a million identical restriction fragments for any of the given gene, so probing a genomic Southern with a gene-specific probe will produce the pattern of perhaps one or of just a few bands.
What are the hormones produced by the testicles and the ovaries? The testicles make androgenic hormones, the major of them being testosterone. The ovaries make estrogen and pro
Define Reagents for Determination of the Iodine Number of Lipids? The following reagents are required to conduct this experiment. 1. Hanus solution (Iodine monobromide solut
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Explain Negative Staining Technique? Here, a stain has a negatively charged chromophore that is repelled by the negative bacterial surface. It results in indirect staining of m
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Explain Hazard Hazard : A biological, chemical or physical agent that is reasonably likely to cause illness or injury in the absence of its control.
What is the minimum sample weight, at USP standards (U = 0.1% and k = 3), that can be measured on an analytic balance in which the repeatability is 0.0001 g. Include the uncertaint
Bovine ephemeral fever Ephemeral fever is commonly known as 'three-day sickness'. It affects mainly cattle and occasionally sheep in India. Causative agent is a mosquito-borne vir
Define Hydroxybenzoic acid and Hydroxycinnamic acid? Hydroxybenzoic acid: e.g. ellagic and gallic acids, which are hydrolyzable tannins, present in berries and nuts. Hydroxy
Determine the use of natural colourants The use of natural colourants is limited due to their instability, low tinctorial power or price disadvantage. The trend towards natura
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