General protocol for media preparation, Biology

Assignment Help:

General Protocol for Media Preparation

A general protocol for media preparation is as follows:

1. Prepare stock solutions one day before the medium is to be made.

2. Weigh the required quantities of agar and sucrose and dissolve them in water (about 314th the final volume of the medium) by heating in a-water bath or autoclaving at low pressure.

3. Pipette the required volumes of each of the stock solutions into the above solution kept on a stirrer.

4. Make up the final volume of the medium by addition of distilled water.

5. Adjust the pH to 5.8 with 0.1-0.5 N NaoH or Hcl.


Related Discussions:- General protocol for media preparation

Microbiology, general characteristics of cynobacteria

general characteristics of cynobacteria

Why does bark often die and break naturally, Why does bark often die and br...

Why does bark often die and break naturally? The bark is the mature periderm of the stem, branches and roots. It dies and breaks when these structures grow and thus the perider

Dietary recommendations proposed by whom, Q. Dietary recommendations propos...

Q. Dietary recommendations proposed by WHOM? We learnt about their etiological factors, metabolic alterations, clinical anifestations and dietary management of the disease of h

What is the intermediate host of schistosoma mansoni, Q. What is the interm...

Q. What is the intermediate host of Schistosoma mansoni? Where does that host live? The intermediate host of a schistosome is a gastropod mollusc, Biomphalaria genus and a snai

Gene interactions (post mendelian genetics), GENE INTERACTIONS (POST MENDEL...

GENE INTERACTIONS (POST MENDELIAN GENETICS) Phenotypic expression of a gene may be altered by the influence of other genes. The modification of the normal phenotype of certain

Name the term for cancer of the blood, Which of the below terms is also cal...

Which of the below terms is also called "cancer of the blood" - an uncontrolled, greatly accelerated production of white cells. Is it: a) Leukemia b) Polycythemia c) sick

What are age pyramids, What are age pyramids? Age pyramids are graphica...

What are age pyramids? Age pyramids are graphical representations in form of superposed rectangles every representing the number of individuals contained in age ranges into whi

How white blood cells are collectively referred, The various types of white...

The various types of white blood cells are sometime together referred to as: a) Erythrocytes (pron: eh-rith-row-cites) b) Erythroblasts (pron: eh-rith-rah-blast) c) Leuko

Differences between vertebrates and the other chordates, What are the diffe...

What are the differences between vertebrates and the other chordates? Vertebrates are dissimilar because they have a spinal column (vertebral column). In these animals the noto

Short note on glycogen storage diseases, Write short note on glycogen stora...

Write short note on glycogen storage diseases. Glycogen storage diseases are caused by genetic defects  that  result in deficiencies in certain enzymes of glycogen metabolism.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd