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Gene targeting: There is always a debate on gene integration in to the host animal.
It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.
Heartwood differs from sapwood in: 1. Presence of rays and fibres 2. Absence of vessels and parenchyma 3. Having dead and non-conducting elements 4. Being susceptible t
Biotin Biotin forms long, colourless needles or a white crystalline powder. It is sparingly soluble in cold water, but more soluble in hot water and dilute alkalis. Biotin is s
Why is it not correct to assert that DNA self-replicates? DNA is not completely autonomous in its duplication process because the replication does not happen without enzymatic
#applications of biotechnology..
Describe three mechanisms cells use to produce diversity of ion channel structure and function.
What is the difference between recessive allele and dominant allele? The Dominant allele is the allele that determines phenotypical features that manifest in heterozygous or ho
What is sporopollinin?
Modes of Respiration Many small organisms obtain oxygen by diffusion through their body surfaces. They do not have any specialised respiratory organs nor do they have blood ci
site of fertilistion in human
Stems transport liquids (a) Cut about 2 cm from the end of stems of celery and place them in cold water for about an hour to freshen. Next place the stems in dishes having red
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