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Gene targeting: There is always a debate on gene integration in to the host animal.
It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.
Q. What are mycotoxins? How are these produced and where are these found? Are these naturally-occurring or produced? What is their significance? First what are mycotoxins?
how oviary work
Primary Egg Envelopes These are the egg envelopes which develop in the ovary between oocyte and follicle cells in the space occupied by the interdigitating microvilli. Such e
Most of the water taken up by a plant passes through it and is evaporated to the atmosphere.What use is made of the tiny fraction of this water which is retained by the plant?
Define effect on Human chorionic gonadotropin in Pregnancy? Human chorionic gonadotropin (HCG) begins to increase immediately on implantation of the ovum and reaches a peak at
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Q. DNA damaged by alkylating agents? 1. Some simple alkylating agents 2. Illustrations of products of alkylating agents 3.Many of these products can be repaired by excision rep
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Define Light Source of Microscope? It is either mirror or electric illuminator present at the base. Identify the mirror in Figure. Some microscopes have reversible mirror with
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