Gene targeting, Biology

Assignment Help:

Gene targeting: There is always a debate on gene integration in to the host animal.

It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.


Related Discussions:- Gene targeting

Determine the number average molecular weight of bag, 1.  You have two bags...

1.  You have two bags of polymer. Bag A has 10 kgs of polymer with weight average molecular weight of 336.6 kg and Bag B has 20kg of polymer with weight average molecular weight 39

Sexology, what is meant by double penetration sex?

what is meant by double penetration sex?

Sedimentation, Simple sedimentation: The process of removal of suspende...

Simple sedimentation: The process of removal of suspended, colloidal impurities by allowing water to stand undisturbed in big tanks about 5m deep. Most of the suspended particl

Define role of zinc in controlling gene expression, Define role of Zinc in ...

Define role of Zinc in controlling Gene Expression? Zinc atoms have specific structural roles in enzyme molecules, as well as, in many other proteins and in bio membranes. Thes

Nutritional requirements of fats and oils during pregnancy, Define Nutritio...

Define Nutritional Requirements of Fats and Oils during Pregnancy and Lactation? ICMR (1990) recommends that linoleic acid requirements should be raised to 4.5 en% during pregn

Explain phylum coelentrat, Phylum Coelentrat a : (9,000 'species) The ...

Phylum Coelentrat a : (9,000 'species) The body is radial and made up of two cell layers. Gastro-Vascular cavity is saclike, tentacles and stinging cells are present. They may

Theory of natural selection, What key idea, contained in Malthus's essay on...

What key idea, contained in Malthus's essay on populations, helped Darwin formulate his theory of natural selection?

Phytochrome - development of plant, Phytochrome - Development of plant ...

Phytochrome - Development of plant You know that plants capture light energy during photosynthesis, now you are familiarised with another important and interesting role of lig

What is meant by the law of use and disuse, What is meant by the law of use...

What is meant by the law of use and disuse and by the law of the transmission of acquired characteristics? As per to the law of use and disuse the characteristics of a body var

The halstead-reitan neuropsychological battery, The halstead-reitan neurops...

The halstead-reitan neuropsychological battery The beginnings of the battery can be traced to the special laboratory established by Halstead in 1935 for the study of neurosurgi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd