Gene targeting, Biology

Assignment Help:

Gene targeting: There is always a debate on gene integration in to the host animal.

It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.


Related Discussions:- Gene targeting

Reproduction, write about asexual reproduction in mucor?

write about asexual reproduction in mucor?

Pisces fish, description on characteristics of pisces fish

description on characteristics of pisces fish

Draw a diagram of transverse section of the spinal cord, Draw a diagram of ...

Draw a diagram of transverse section of the spinal cord showing reflex are and label the following parts: (i) Afferent nerve fibre (ii) Grey matter (iii) Motor nerve fibre

Explain glucokinase, Glucokinase In the fed state, clearance of blood  ...

Glucokinase In the fed state, clearance of blood  glucose is mainly by  liver via glucokinase. Glucokinase, which  is an  inducible enzyme, removes most of the blood glucose fr

How age factors affect the requirement of protein, How age factors affect t...

How age factors affect the requirement of protein? Age: Protein in excess of maintenance needs is required, when a new tissue is being fornled. Certain age periods, when growth

While meiosis results in four diploid cells, Which of the following best co...

Which of the following best compares the results of mitosis and meiosis? Mitosis results in four diploid cells, while meiosis results in two haploid cells. Mitosis results in four

Flower, flowers formation without any leaves?

flowers formation without any leaves?

What is post myocardial infarction ventricular septal defect, What is Pos...

What is Post Myocardial Infarction Ventricular Septal Defect ? Post Myocardial Infarction Ventricular Septal Defect (Septal Rupture) The correct terminology is septal

Explain the procedure estimation of the amount of bacteria, Explain the Pro...

Explain the Procedure Estimation of the Amount of Bacteria? Now carry out the exercise following the steps enumerated herewith. 1. Prepare ten fold dilutions of E. coli cult

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd