Gene targeting, Biology

Assignment Help:

Gene targeting: There is always a debate on gene integration in to the host animal.

It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.


Related Discussions:- Gene targeting

Bacteria-virus-types of procaryotes, Bacteria: Bacteria have a protect...

Bacteria: Bacteria have a protective covering called 'cell wall'. Beneath this. there is a plasma membrane which encloses protoplasm of the bacterium containing various types

Assessment of megaloblastic anaemia, Assessment   On observation of a c...

Assessment   On observation of a child with this type of anaemia you will find that in addition to the symptoms  of iron deficiency anaemia there is hyper-pigmentation over knu

1, what are the variations of the digestive system in animals? what musthav...

what are the variations of the digestive system in animals? what musthave caused these variation

How to decreases the rate limiting, When the product of a metabolic pathway...

When the product of a metabolic pathway decreases the rate limiting earlier in the pathway it is called?

Determine the concept of imitation in the learning process, Determine the c...

Determine the concept of  imitation in the learning process The concept of  imitation in the learning process is also a key developmental concept for infants and young children

How are radioactive probes useful in dna fingerprinting, How are radioactiv...

How are radioactive probes useful in DNA fingerprinting? Radioactive probes bind to specific fragments in a DNA sample like a donor gene. A probe can identify which bacteria ha

Energy storage, Energy Storage As we said above, food intake and ener...

Energy Storage As we said above, food intake and energy expenditure for animals is approximately equal. If energy expenditure exceeds food intake, then the excess energy is t

How do cells obtain energy for their functioning, How do cells obtain energ...

How do cells obtain energy for their functioning? Cells obtain energy for their metabolic reactions from the breaking of organic molecules with high energetic content. This ene

What are the different organ of the gastrointestinal system, The different ...

The different organs of the gastrointestinal system  Mouth or buccal cavity - Teeth 32 Permanent and - Tongue  Pharynx  Oesophagus  Stomach  Small Intestine

What is the thermopenetration concept, What is the thermopenetration concep...

What is the thermopenetration concept? The pasteurization of products packed in glass containers leads to a problem of a specific nature, which is referred to as 'thermopenetra

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd