Gene targeting, Biology

Assignment Help:

Gene targeting: There is always a debate on gene integration in to the host animal.

It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.


Related Discussions:- Gene targeting

Why roots of many swamp plants have a special morphology, Why roots of many...

Why roots of many swamp plants have a special morphology? The Swamp and The marsh plants in general present supporting roots that ramify from portions of the stem above the gro

Excreatory organ, what is the excreatory organ of silverfish?

what is the excreatory organ of silverfish?

Animal biodiversity, Discuss why Obelia is considered to be of special inte...

Discuss why Obelia is considered to be of special interest in Zoology as an animal showing an intermediate grade of organization.

Explain the working of pulmonary circulation, Explain the working of Pulmon...

Explain the working of Pulmonary Circulation? In pulmonary circulation, blood is pumped to the lungs, where carbon dioxide is exchanged for oxygen. Blood returning from the bod

Define endocrine or hormonal disorders - reproductive tract, Define Endocri...

Define Endocrine or Hormonal Disorders affect the Reproductive Tract Endocrine or hormonal disorders can affect several aspects of reproduction, from menstruation to fertility.

What are the other substances resorbed by nephron tubules, Where does most ...

Where does most of the water resorbed after glomerular filtration go? What are the other substances resorbed by the nephron tubules? Only 0.5 to 1% of the glomerular filtrate i

Explain the filtration - air sampling, Explain the Filtration - Air Samplin...

Explain the Filtration - Air Sampling? Filtration - Air stream may also be filtered through a micro-filter.  Microorganisms are released from the filter using a suitable diluen

Find the genotypes and phenotypes of the f1, Determine the genotypes and ph...

Determine the genotypes and phenotypes of the F1 generation from a colour blind father and a mother who is homozygous for normal colour vision.

Stomata can open and close in response to changes in the co2, Stomata can o...

Stomata can open and close in response to changes in the CO 2 concentration inside the leaf. Would you expect stomata to open or close if the CO 2 concentration decreased? Explai

Endoplasmic reticulum, ENDOPLASMIC RETICULUM (ER) Eucaryotic celIs have t...

ENDOPLASMIC RETICULUM (ER) Eucaryotic celIs have two major compartments- nucleus and cytoplasm. Cytoplasm was known to have no structure until the discovery of electron micrsscop

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd