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Gene targeting: There is always a debate on gene integration in to the host animal.
It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.
Organic Molecules Some organic molecules in life are Carbohydrates and Lipids à C, H and O Proteins à C, H, O, N & sometimes S Nucleic acids à C, H, O, N & P
When ATP gives energy to the cellular metabolism it loses one of its phosphates and ADP reappears. ADP can also lose more phosphates and produce AMP (adenosine monophosphate) o
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The components of treatment of NKHDC are: 1) control of water loss. 2) control of sugar using insulin. 3) adjust electrolytes like sodium and potassium. 4) control infe
Is the biological species concept subjective or objective? How about the phylogenetic species concept or is it subjective or objective?
HMP shunt in erythrocytes HMP shunt in erythrocytes is of importance due to the generation of NADPH, which maintains the glutathione (G-SH) in the reduced state by glutathi
Spermiogenesis - Spermatogenesis At the end of the meiosis the spermatids appear as simple spherical cells with a centrally located nucleus. Their differentiation into sperm r
Explain the History of Plant Classification? Before Darwin's theory of evolution and publication of his epoch making work 'Origin of Species' in 1959 no outstanding basis for
What is reflexive memory? Reflexive memory relies on the cerebellum and amygdala. Compare among aerobic respiration and anaerobic respiration. Aerobic respiration happens in th
Explain the Bunsen Burner? It is a type of gas burner that gives very hot flame by allowing air to enter at the base and mix with the gas. It is used - (a) For sterilizing i
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