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Gene targeting: There is always a debate on gene integration in to the host animal.
It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.
Ears We have a pair of ears. Each ear has three parts - external, middle and inner ear. The function of the middle ear is transmission of sound waves from external to inner ear
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Consider the following four statements (a - d) regarding kidney transplant and select the two correct ones out of these. 1. Even if a kidney transplant is proper the recipient m
Biotin Biotin forms long, colourless needles or a white crystalline powder. It is sparingly soluble in cold water, but more soluble in hot water and dilute alkalis. Biotin is s
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