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Gene targeting: There is always a debate on gene integration in to the host animal.
It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.
Q. What are the three fundamental sexual life cycles studied in Biology? Which of them corresponds to metagenesis? Which of them is the human life cycle? Sexual reproduction ma
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How bone implant interface can be evaluated 1) Evaluation of the bone - implant interface can be done by following: i) Implant mobility and discomfort a) Clinical implan
are they diploblastic or triploblastic
Cryopreseravtion of gametes , embryos or DNA segments can be quite an effective and safe approach for breeds or strains whose populations are too small to be conserved by any other
What are the disadvantages of protozoa
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I have 30µg of a primer, whose molecular weight is 600. How much water should I add to get a stock primer concentration of 100µM?
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