Gene targeting, Biology

Assignment Help:

Gene targeting: There is always a debate on gene integration in to the host animal.

It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.


Related Discussions:- Gene targeting

What do you understand by the term accommodation, (a) What do you understan...

(a) What do you understand by the term 'accommodation'? (b) What part does the lens play in this process?   a) Accommodation is the way the eye can focus either

Aortic valve replacement-surgical techniques, Aortic Valve replacement ...

Aortic Valve replacement :  The initial steps of the operation have been described earlier. Ascending aorta is cannulated. A single two-stage atrio-venous cannula is inse

Glucose-6-phosphate to glucose, Glucose-6-phosphate to Glucose Glucose...

Glucose-6-phosphate to Glucose Glucose-6-phosphate to Glucose : Glucose-6-phosphate  is converted to glucose  by glucose-6-phosphatase which is present in intestine, liver and

Causes of variation, CAUSE S OF VARIATION - Effect of environmental co...

CAUSE S OF VARIATION - Effect of environmental conditions Change in the gene pattern like - (a) Random distribution of homologous genes in meiosis (b) Crossing over

Structure and content of neuropsychological battery, Structure and Content ...

Structure and Content of NEUROPSYCHOLOGICAL BATTERY The battery contains 269 items, each of which may be scored on a 2- or 3- point scale. A score of 0 indicates normal perform

Explain iron uptake by cells, Explain Iron Uptake by Cells? Iron partic...

Explain Iron Uptake by Cells? Iron participates in a large number of biochemical reactions. However, for iron to perform any function, it first needs to be taken up by the cell

Define fuel molecules and lipogenesis, Define Fuel Molecules and Lipogenesi...

Define Fuel Molecules and Lipogenesis? Lipogenesis is a collective name for the complex process of producing lipids (fatty acids) from smaller precursor molecules. The synthesi

Overgrazing - degradation of ecosystem, Overgrazing - Degradation of Ecosys...

Overgrazing - Degradation of Ecosystem It refers to the condition when the grazing pressure on the vegetation is so intense that it does not recover. Ultimately, there is a lo

Growth charts, Growth Charts Management alone without any standard of ...

Growth Charts Management alone without any standard of comparison do not serve useful purpose. A number of standards have been developed to compare the measurement of any

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd