Gene targeting, Biology

Assignment Help:

Gene targeting: There is always a debate on gene integration in to the host animal.

It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.


Related Discussions:- Gene targeting

Ctenophora.., Ask queAffinities of ctenophora with platyhelminthesstion #Mi...

Ask queAffinities of ctenophora with platyhelminthesstion #Minimum 100 words accepted#

Define discovery of other essential nutrients, Define Discovery of other Es...

Define Discovery of other Essential Nutrients? Following the identification of the food factors involved in beriberi, pellagra and scurvy, the search was on for other water-sol

Description and measurement of circadian rhythms, The description and measu...

The description and measurement of circadian rhythms. Describe the standard method used for the recording and graphing of behavioral rhythms in animals, especially the use of

Why the inflammatory response is considered a body, List specific reasons w...

List specific reasons why the inflammatory response is considered a body defense mechanism.

Define human beings need energy for basal metabolism, Define human beings n...

Define human beings need energy For Basal metabolism? This comprises a series of functions that are essential for life, such as cell function and replacement; the synthesis, se

Define functions of magnesium - macro minerals, Define functions of magnesi...

Define functions of magnesium - Macro minerals? Like Ca, Mg too has a role in bone formation. Soft tissue magnesium functions as a cofactor of many enzymes involved in energy m

Describe two differences among green algae and red algae, Some biologists p...

Some biologists prefer to categorize brown, red, and some green algae as plants rather than protists. What characteristics of these algae support like classification? Brown and

Signify the term - proglottids, Signify the term - Proglottids? Seriall...

Signify the term - Proglottids? Serially repeating segment like structures found in tapeworms which contain reproductive organs. Immature proglottids are continually added at t

Advances in mineral and vitamin nutrition of livestock, Advances in Mineral...

Advances in Mineral and Vitamin Nutrition of Livestock Even though, minerals and vitamins as nutrients are as essential as energy and protein, general negligence occurs primari

Toxoplasma gondii - protozoan, Toxoplasma gondii Another well known Sp...

Toxoplasma gondii Another well known Sporozoan is Toxoplasma gondii which has an unusually wide host- range for n protozoan parasite. It can probably infect all warm-blooded a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd