Gene targeting, Biology

Assignment Help:

Gene targeting: There is always a debate on gene integration in to the host animal.

It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.


Related Discussions:- Gene targeting

Physiology, Slacklining is the act of walking across a suspended nylon stra...

Slacklining is the act of walking across a suspended nylon strap tied between two vertical anchor points. List the senses used to maintain an upright position while slacklining an

Deficiency diseases-neonatal hypoglycaemia, Neonatal  hypoglycaemia Hy...

Neonatal  hypoglycaemia Hypoglycaemia is a metabolic condition of newborn piglets that develops in first few days of life due to decreased caloric intake and increased cataboli

What is ascending aortic aneurysm, What is Ascending Aortic Aneurysm ? ...

What is Ascending Aortic Aneurysm ? Ascending Aortic Aneurysm :  In aneurysms confined to ascending aorta well below the innominate artery origin, the technique used is

Explain functional properties of food lipids, Functional properties of food...

Functional properties of food lipids Chemically, fats and oils, as you may already know by now, are a combination of glycerol and fatty acids. The glycerol molecule has three s

Zoonoses disease-leptospirosis, Leptospirosis Leptospirosis is a gener...

Leptospirosis Leptospirosis is a general term that denotes all infections of man and animals caused by spirochaetes of the genus Leptospira. At one time, the infection had bee

What are the major complications of diabetes mellitus, Q. Why do diabetic p...

Q. Why do diabetic patients often undergo dietary sugar restriction? What are the major complications of diabetes mellitus? Diabetic patients are frequently advised to ingest f

Explain polio, Explain Polio Adults who have not previously been immuni...

Explain Polio Adults who have not previously been immunized against polio should receive a primary series of inactivated polio vaccine (IPV) if traveling to areas where polio i

What are the three main parts of a eukaryotic cell, What are the three main...

What are the three main parts of a eukaryotic cell? The eukaryotic cell can be divided into two major portions: the cell membrane that divides the intracellular space from the

Reproduction in cnidarians, How does metagenesis in cnidarians differ from ...

How does metagenesis in cnidarians differ from the true alternation of generation shown by plants?

Importance of counselling for diabetic patient, Q. Importance of counsellin...

Q. Importance of counselling for diabetic patient? Diabetes is a life-long illness. Life style modification and precautions taken by the patient can prevent complications and i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd