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Gene targeting: There is always a debate on gene integration in to the host animal.
It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.
Normal or General Diet This diet is planned to be consistent with the Recommended Dietary Allowances (RDAs) of nutrients and is based on the food groups. It is usuall
Renal parenchymal disease is the commonest cause of secondary hypertension. Hypertensive and diabetic nephropathy and chronic glomerulonephritis are the main causes. Eventually, t
(a) What effect does alcohol have on reaction time? (b) What other short-term effects does alcohol have? (c) What long-term effects can result from an exce
Describe the appearance of a single E. coli colony. Why can it be considered genetically homogenous?
Controlling Introduction of Alien Species As you know the alien species can cause the existing population to decrease or even become extinct. The individuals of alien species
what are autoimmunedisorders?
Founder effect The difference in gene pools amongs an original population and a new population founded by one or a few individuals randomly separated from the original population,
Basic Instruments /Equipment Used In biochemical laboratories and important Working tips The first practical in this manual will orient you to the various equipment, apparatuses
a) What are the advantages of human milk over cows' milk for feeding babies? b) Apart from the composition of the milk, what are the other advantages of breast- feeding?
Cost due to Injury to Worker Medical cost - medicine, surgery, hospitalization, extended treatment. Working time lost due to injury or death. Time loss of cowork
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