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Gene targeting: There is always a debate on gene integration in to the host animal.
It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.
Determine requirements of Vitamins at high altitude? It is observed that requirement of vitamins is not different as compared to plains. In humans, exposure to high altitude ha
You need to prepare 500 ml of a solution containing 10 mM Tris, 0.15 M NaCl and 1 mg/ml SDS. At your work disposal are stock solutions containing 1 M Tris, 2.5M NaCl and 10% (w/v)
Cinemicrography Earlier it was impossible to follow the evenvts within the cell due to: i) non-visibility of cell organelles under a light microscope, ii) maintenance of cells
Structure and development of leaf
Placenta - Human Development We had said previous in the unit that in the second week of development a primitive uteroplacental circulation is established. Early in this stage
A mother has brought her 2-week-old infant to the emergency department due to the baby's persistent and increasing jaundice. Blood testing reveals that the infant's unconjugated bi
The vertebral bodies are much larger in the lower back than the neck. What is the functional significance of this structural difference?
How Poor Selection of Food causing the Underweight? Poor Selection of Food: Poor selection of food along with irregular eating habits may be responsible for insufficient food
PKU is a recessive disorder. Suppose two people who were heterozygous for PKU married and had a child. What is the probability that the child will have PKU?
How is energy transferred along a food chain? The energy flux beside a food chain is always unidirectional, from the producers to the decomposers.
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