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Gene targeting: There is always a debate on gene integration in to the host animal.
It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.
Social Basis of Primitive Life: In the previous section, we gave you a glimpse of how the primitive human beings faced the problems of day to day existence and found some so
A person is declared to be dead upon the irreversible cessation of spontaneous body functions: brain activity, or blood circulation and respiration. However, only about 1% of a per
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What is reproductin
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What is the best microscope to get a detailed view of the parts inside of a preserved plant cell?
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Where does cartilage occur in a joint and what it its function? Cartilage might be found covering the surface of bones where they meet in a movable joint. Cartilage decreases f
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