Gene targeting, Biology

Assignment Help:

Gene targeting: There is always a debate on gene integration in to the host animal.

It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.


Related Discussions:- Gene targeting

Nursing care - megaloblastic anaemia, Planning the Nursing Care   The g...

Planning the Nursing Care   The goals of nursing care are:    Identify the causative factor of megaloblastic anaemia.    Administer appropriate vitamins depending  on

Give the results of distae dissection , Give the results of DistaE Dissecti...

Give the results of DistaE Dissection ? Results :  For acute dissection of different parts of aorta, the mortality is reported as 9 to 33 per cent. In the International Regis

Etiologic factor of atherosclerosis, Q. Etiologic factor of atherosclerosis...

Q. Etiologic factor of atherosclerosis? Various factors are responsible for atherosclerosis. These include: 1. Hyperlipidemin: Excess circulating fats in blood especially th

Nucleic acids, Why is the number of bonds important in nucleic acids

Why is the number of bonds important in nucleic acids

Digestion of proteins, Digestion  of proteins Digestion  of proteins  ...

Digestion  of proteins Digestion  of proteins  is  initiated  in  the  stomach by pepsin.  Pepsin  acts upon food proteins  and  converts them  to proteoses  and  peptones whic

The rapeutic nurse, THE RAPEUTIC NURSE: Development of  therapeutic  n...

THE RAPEUTIC NURSE: Development of  therapeutic  nurse patient  relationship is the basic requirement  of nursing. A helping relationship  in nursing may be explained as an in

Define other sex determination systems, Besides the XY system are there oth...

Besides the XY system are there other sex determination systems? A few animals have a sex determination system different from the XY system such as the X0 system is the sex det

Membrane potential, a solution of 5mMol/L CaCl2 is separated from a solutio...

a solution of 5mMol/L CaCl2 is separated from a solution of 1 micromole/L CaCl2 by a membrane that is selectively permeable to Ca+2 but is impermeable to Cl-1 what are the magnitu

What are the dominate phenotype, There is a population of 100 frogs. 20 are...

There is a population of 100 frogs. 20 are heterozygotes, 30 are homozygous recessive, 25 are homozygous dominate. What is the frequency of the dominate allele? The recessive allel

What is physiologic adaptation, Physiologic Adaptation Modeling is a su...

Physiologic Adaptation Modeling is a surface specific activity (apposition or resorption) that produces a net change in the size and/or shape of the bone. It is an uncoupled pr

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd