Gene targeting, Biology

Assignment Help:

Gene targeting: There is always a debate on gene integration in to the host animal.

It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.


Related Discussions:- Gene targeting

Chemical stress - nature of the solutes, Chemical Stress - Nature of the so...

Chemical Stress - Nature of the solutes The acidic or basic reaction of soil and water of a particular habitat reflects its geochemical history beginning with its formation an

Effect of a deletion in the operator of the lactose operon, Compare and con...

Compare and contrast the effect of a deletion in the operator of the lactose operon with one in the operator of the histidine operon.

Functions of endoplasmic reticulum, FUNCTIONS OF E.R. (1 )      Mecha...

FUNCTIONS OF E.R. (1 )      Mechanical support - E.R. participates in formation of cytoskeleton with microfilaments, microtubules and intermediate filaments. (2 )

Define absorption, Define Absorption, Storage and Elimination of Vitamin K?...

Define Absorption, Storage and Elimination of Vitamin K? Dietary vitamin K, mainly phylloquinone, is absorbed chemically unchanged from the proximal intestine after solubilizat

History of the cell, HISTORY OF THE CELL Term "Cytology" was given b...

HISTORY OF THE CELL Term "Cytology" was given by Hertwig , he also wrote a book on " Cell and Tissue ". Father of cytology = Robert Hooke. Father of Modern cytology

Class of coclentrata, ????? # 100 ??????????? #Minimum ?????? ?????

????? # 100 ??????????? #Minimum ?????? ?????

Define influence of polyphenols on carbohydrates, Define influence of polyp...

Define influence of polyphenols on Carbohydrates? Binding of proteins may indirectly affect carbohydrate absorption. Inhibition of amylolytic enzymes and subsequent reduction o

What do you understand by term - acanthus, What do you understand by term -...

What do you understand by term - acanthus The egg and larval phase of an acanthocephalan which passes from the female parasite to the feces of host.

Nucleic acids, Why is the number of bonds important in nucleic acids

Why is the number of bonds important in nucleic acids

Cytoplasm plays the most important pathogenetic role, The formation of surf...

The formation of surface blebs caused by ischemia reflects in part plasma membrane injury, but also a sign of cytoplasmic injury. Which filamentous component of the cytoplasm plays

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd