Gene targeting, Biology

Assignment Help:

Gene targeting: There is always a debate on gene integration in to the host animal.

It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.


Related Discussions:- Gene targeting

Implications of pharmacogenomic research, Problem 1: Describe the funct...

Problem 1: Describe the functions of: a. Glutathione transferases b. Sulfotransferases. -Describe the functions of: a. Glutathione transferases Describe the functions of

Risk analysis - animal studies, Animal studies Most toxicological data ...

Animal studies Most toxicological data for risk assessment are derived from the animal studies and  it  is, therefore, essential that these studies be conducted following widel

What do you understand by abdomen, In organisms that have undergone tagmosi...

In organisms that have undergone tagmosisthe trunk often develops into two tagma, oneinvolved in locomotion and one that is notlocomotory. The tagma not involved in locomotionis re

Fisson, what is mean by fisson

what is mean by fisson

Explain nutrition monitoring and evaluation, Nutrition Monitoring and Evalu...

Nutrition Monitoring and Evaluation Monitoring and evaluation is an essential step in the nutritional care process and  is very important too. Monitoring specifically refers to

Contagious bovine pleuropneumonia (cbpp), Contagious bovine pleuropneumonia...

Contagious bovine pleuropneumonia (CBPP) This is a highly infectious disease and is of major economic importance in certain tropical countries. The causative organism is Mycop

Functions of insulin, FUNCTION S - (a) It is antagonistic to glucagon....

FUNCTION S - (a) It is antagonistic to glucagon. Insulin decreases the level of glucose in the blood. It acts by increasing the rate at which glucose is transported out of the

Excretory system, what is the excretory organ of agama lizard

what is the excretory organ of agama lizard

Excretory organ, What is the excretory organ of lizard

What is the excretory organ of lizard

Explain third mitotic period, Q. What are the major events of the third mit...

Q. What are the major events of the third mitotic period? The third mitotic period is anaphase. In anaphase the following events occur breaking and duplication of centromeres w

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd