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Gene targeting: There is always a debate on gene integration in to the host animal.
It is random gene integration Vs gene targeting. Perhaps most significantly, pro-nuclear injection cannot be used to modify the animals' own genes. By contrast, target modifications to endogenous genes have been achieved earlier in mouse model and now in farm animals also. When introduced into the early embryo these cells contribute to the germline. They can also undergo homologous recombination with transfected foreign DNA so that precise, targeted changes can be introduced. The combination of these two properties means that gene targeting of the mouse germline is now routine, allowing for the deletion, replacement or modification of selected genes. Recombination on either side of the marker gene results in its precise insertion into the chromosome at the same time and delete some or the entire target gene. Precise targeting events are very rare (perhaps 1 in 106 cells) and, therefore special methods must be taken for identifying and selecting the correctly targeted cells.
Q. How Drying used for sterilization? Moisture is essential for bacteria, drying therefore has a deleterious effect on most bacteria. Spontaneous drying can often kill bacteria
What is Coelom - Body Cavity explain? Among the bilaterally symmetrical organisms, animals have three different types of body plans with respect to body cavities. The term coel
working of pcr
Reaction specificity Some enzymes catalyze only one reaction acting on a specific substrate. Example: urease and catalase acts only on urea and hydrogen peroxide,
Microfilaments - Role of Cytoskeleton Structures Microfilaments are as well present in all animal cells. They are found singly throughout the cytoplasm organized like a meshwo
Explain the Interaction of Riboflavin with other Nutrients? Riboflavin, as already discussed under the functions, interrelates with other B vitamins, notably niacin, which r
What is Choanoflagellate? Explain in brief. The protozoans which resemble the choanocyte cells found in sponges. They have a collar of microvilli which surround a central flage
Q. Explain Consciousness as inner state? Consciousness as inner state: We are conscious of thoughts, images, emotions and memories within ourselves though they may not have phy
How is hydration control by active pump mechanisms responsible in the corneal hydration? Hydration Control by Active Pump Mechanisms: a) Na/K ATPase pump, which is more acti
Slow Moving Waters - Biota of Rivers The habitat of a slowly moving part of the river is very different from the one just described. Here the water flow is comparatively slow
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