Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

#protozoa, #what is the habitat of phylum protozoa

#what is the habitat of phylum protozoa

Biological species concept, The biologicaI species concept claims that spec...

The biologicaI species concept claims that species consist of natural populations and that species are real and objective. They are not man-made subjective abstraction. According

Rectum examination of new born, Rectum check for anal patency and p...

Rectum check for anal patency and passage of meconium and observe whether meconium is passed or not. Confirm its patency then pass a catheter or gloved finger  check if

Explain prophylaxis with antimicrobial, Explain Prophylaxis with antimicrob...

Explain Prophylaxis with antimicrobial Prophylaxis with antimicrobials has decreased the incidence of surgical site infection after head and neck operations that involve an inc

Aeolian-transported soil, Aeolian Wind transported materials constitute...

Aeolian Wind transported materials constitute this category. This can be further divided into dunes or loess. Dunes are found in thr& types of situations. Fitly, these may occu

Type one diabetes mellitus, Q. Type one Diabetes Mellitus? Type 1 DM oc...

Q. Type one Diabetes Mellitus? Type 1 DM occurs in children and adolescents (6-14 yrs). In this disease, the body makes little or no insulin. Daily injections of insulin are ne

Determine the viscosity of solutions, Viscosity Viscosity, as you may ...

Viscosity Viscosity, as you may already know, is associated with fluid flow.  It is the internal friction which tends to bring to rest portions of the fluids moving relative to

Define the terms transcriptomics and transcriptome, 1. One of the greates c...

1. One of the greates challenges following the human genome project is to understand how genes are regulated and what functions they perform. a. Define the terms 'Transcriptomic

Which statement is false, Which is FALSE? Diastereomers: Select one: ...

Which is FALSE? Diastereomers: Select one: a. Have the same molecular weight b. Have the same connectivity c. Are mirror images d. All of the above e. None of the

What happens when the ovum is fertilized, What happens when the ovum is fer...

What happens when the ovum is fertilized? If the ovum is fertilized, there is no breakdown of endometrium and no menstrual flow. The fertilized ovum travels through uterine tub

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd