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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
What is NAD? What is the role of the NAD molecule in glycolysis? NAD (nicotinamide adenine dinucleotide) is a hydrogen acceptor essential as reductant (to receive hydrogen) in
proteins contain
Mention the product and its use formed by each the microbes listed below: i) Lactobacillus ii) Streptococcus iii) Saccharomyces cerevisiae
how i prepare my best assignment on DNA Polymerase 1 2 and 3
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Ovary is half-inferior in the flowers of : 1. Guava 2. Plum 3. Brinjal 4. Cucumber Plum is the flower in which ovary is half - inferior
Explain the Scaling from individuals to Ecosystems? Models that explain how individual organisms acquire energy and materials, and how they use them for survival, growth and re
Define Lipids - Tests for Presence of Exoenzymatic Activity? Lipids are also high molecular weight compounds. Enzyme lipases (esterases) cleaves the ester bond to form glycerol
Give three examples of cell structures which contain structural proteins. What is the other type of protein in a cell? (a) Cytoplasm, the cell membrane, memb
1. DNA must be extensively folded in order to fit inside a nucleus that is less than 10 um in diameter. a. Describe how DNA is folded into chromatin structures of increasing com
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