Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Cell body of neuron or perikaryon, CEL L BODY (= CYTON OR SOMA) OR PERIKAR...

CEL L BODY (= CYTON OR SOMA) OR PERIKARYON - It is nucleated & broader part of neuron. It may be up to 13.5 mm in diameter and may be irregular, spherical, oval, rounded

Define chagas and arrhythmogenic right vent cardiomyopathy, Chagas' Cardiom...

Chagas' Cardiomyopathy It is common in south and Central America and is caused by Trypanosoma cruzi. Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) It is mark

Why do plants need to exchange gases with the environment, Why do plants ne...

Why do plants need to exchange gases with the environment? Plants require to do gas exchange because they create aerobic cellular respiration (like animals) and they need to ge

What is atrial fibrillation, Q. What is Atrial Fibrillation ? As the le...

Q. What is Atrial Fibrillation ? As the left atrial size increase and atrial wall gets fibrosed, depolarization wave fronts get fragmented and atrial fibrillation sets in. Atri

Loss-of-function mutations, Choose the correct answer. A. The phenotype det...

Choose the correct answer. A. The phenotype determines the genotype. B. True-breeding individuals are produced by repeated backcrossing. C. Recessive alleles are usually loss-of-fu

Providing critical resources - conservation of wildlife, Providing Critical...

Providing Critical Resources - Conservation of Wildlife Another way to improve the habitat of a threatened species is to determine which resource is limiting the population si

Classification of plant on the basis of their resemblances, Q. Classificati...

Q. Classification of plant on the basis of their resemblances? Pliny seemed to have little interest in the classification of plant on the basis of their resemblances. He classi

Why physical fitness is tested, Why Physical Fitness is Tested? 1) To a...

Why Physical Fitness is Tested? 1) To assess pre-training fitness status: Before beginning an exercise programme and allow the exercise, intensity and duration to be set at th

Estimation of protein by carcass nitrogen analysis method, Define Estimatio...

Define Estimation of protein by Carcass nitrogen analysis method? Carcass nitrogen analysis: This method includes Nitrogen retention method Net protein utilization

Brain, How brain thinks?

How brain thinks?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd