Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

What are living and nonliving reservoirs, What are living and nonliving res...

What are living and nonliving reservoirs? Viruses are both living as well as non-living. They have reservoirs of genes. A one nucleotide is a unit of gene. Viral genes make use

Explain the difference among dna primase and dna polymerase, Which of the f...

Which of the following is not a difference among DNA primase and DNA polymerase? A. DNA primase can initiate replication of DNA de novo whereas DNA polymerase needs a short oli

Temperature - seed dormancy, Temperature - Seed Dormancy Low temperatu...

Temperature - Seed Dormancy Low temperature treatment is an essential prelude to germination in many seeds, and high temperature may be inhibitory at the time of germination.

What are the dietary goals for diabetes, Q. What are the dietary goals for ...

Q. What are the dietary goals for diabetes? Well here they are. These are to: 1. Supply optimum nutrition to maintain good health, 2. Provide calories for maintaining ide

How emulsion are formed, How emulsion are formed The most common method...

How emulsion are formed The most common method of preparing an emulsion is by mechanically dispersing one liquid phase in another by formation of small droplets. This takes pla

Circulation - initiation of cpr, Circulation: If central pulses (femor...

Circulation: If central pulses (femoral  in infants and  carotid in  children) are not  palpable, begin chest compression without losing any time.  In  young infants, encircle

Sexual reproduction in animals, Sexual Reproduction All metazoans, if...

Sexual Reproduction All metazoans, if or if not capable of propagating asexually, produce special cells termed as gametes. The gametes may be either male gametes or sperms or

Explain endonucleases, Explain Endonucleases Endonucleases  are  nucle...

Explain Endonucleases Endonucleases  are  nucleases present  in  the pancreatic juice like RNAase  andDNAase. These hydrolyze internal phosphodiester bonds which are located m

What is the predicted cell morphology, What are the predicted cell morpholo...

What are the predicted cell morphology, cell arrangement and gram stain results for each of the follow? 1. Bacillus megaterium 2. Staphylococcus aureus 3. Serratia marcesc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd