Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

.micro organisms, what are the disadvantages of protozoa

what are the disadvantages of protozoa

Explain about oil-bearing fruits, Explain about Oil-bearing fruits Oil-...

Explain about Oil-bearing fruits Oil-bearing fruits, nuts and seeds have been grown and used for food for many centuries. More than 100 varieties of plants are known to have oi

Which equipments are required for stress laboratory, The stress lab must ha...

The stress lab must have the following equipment: 1) A mercury Blood Pressure apparatus. 2) A Bed so that the patients ECG can be recorded in the recovery period in supine po

Stress of hospitalization, STRESS OF HOSPITALIZATION   Stress refers to...

STRESS OF HOSPITALIZATION   Stress refers to the imbalance between the environmental  and societal demands and the child's coping abilities  and resources that puts  them in a

Explain the process of determination of vitamin C, Determination of vitamin...

Determination of vitamin c Majority of chemical methods of determination is based on the rapid oxidation of ascorbic acid and therefore not too highly specific. Titration of

What is the physiological explanation for archaic method, In ancient Greece...

In ancient Greece the father of Medicine, Hypocrates, described a method of diagnosing diabetes mellitus by tasting the patient's urine. What is the physiological explanation for t

What would the result of a haploid egg, If a diploid sperm fertilized a hap...

If a diploid sperm fertilized a haploid egg, what would the result be? a.Haploid (n) b.Diploid (2n) c.A mixture of diploid (2n) and haploid (n) d.Triploid (3n)

Assignment of botany, habitat, habit, cell wall chemistry, reason why cell ...

habitat, habit, cell wall chemistry, reason why cell wall like this of Archaebacteria , eubacteria,fungi,algae,bryophytes ,pteridophyes, gymnosperms , angiosperms..

Micro, What are the differences between the two domain

What are the differences between the two domain

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd