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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Explain Development of excretory system? The filtration rate of kidney is low and infants find it difficult to eliminate high concentration of solutes. However, by the end of t
Blunt Injury Closed penumothorax Tension pneumothorax Tracheobronchial injury Fracture ribs and flail chest Mediastinal injury
Define Precautions - Estimation of Nitrogen and Protein Content? 1. The apparatus should be airtight to prevent leakage of NH 3 . 2. The tip of the delivery tube should be i
what are viroids
Mutations and genetic recombination (in sexually reproducing organisms) are major sources of variations in natural populations, In this section we will briefly mention the various
What was the experiment of Stanley Miller (1953) on the origin of life? In 1953 the Stanley Miller arranged an experimental apparatus that simulated the atmospheric conditions
Artificial respiration First - aid for drowning victims should aim to restore respiration as quickly as possible. First of all, nose, mouth and throat of the victim shoul
Explain the Autonomic neuropathy It leads to dry skin due to decreased sweating. Dryness of the skin leads to cracking which makes entry of infection in to the deeper plane eas
Place a slide of Amoeba (common species is A. proteus) under tlie lnicroscope and focus it under low power (5x x 5x), and observe it carefully. Amoeba looks like an irregular colou
Explain Risk Assessment Risk Assessment : The scientific evaluation of known or potential adverse health effects resulting from human exposure to food borne hazards
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