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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
he Lac Repressor protein, which we will talk about a bit later in the course, is important for controlling expression of a number of genes in E. coli. There are about 10 copies of
What is Tetralogy of Fallot ? Figure: Anatomical location of tetralogy of fallot Indications for Surgery : The diagnosis of tetralogy is considered as an indicatio
What are the differences between electron acceptors and electron carriers? Give an example of each.
Explain Non-Glycerides in Edible Oils? You may be aware of the fact that the glyceride fraction (an ester of glycerol and fatty acids that occurs naturally as fats and fatty oi
Assortment is a method in which the meiosis produces new combinations of the genetic information. The paternal and maternal chromosomes line up randomly during the process of syna
Define Structural Elements - Biological Functions of Proteins? Structural proteins are fibrous proteins. The well known of the fibrous proteins are possibly the keratins, which
Q. Explain about Oxidation reduction potential? The tendency of a substrate to accept or donate electrons is termed its redox potential (Eh). When a substrate loses electrons,
Salmonellosis in poultry A wide variety of serovars are prevalent among chicken, turkeys, ducks and geese. The poultry is an important reservoir of salmonellae. The common dise
Anomalies Related to Oral Cavity Under these anomalies we will briefly discuss the cleft lip and cleft palate. You must have seen and/or nursed a baby born with a cut on
Insulin is released into the bloodstream by the β cells of the pancreas when blood glucose stages are high after feeding and stimulates glycogen synthesis to kept exces
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