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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Surgical Considerations in anterior mandibular region During the harvesting of a monocortical symphyseal block of bone from the mandibular symphysis region (autogenous block bo
Explain about Hypothalamus Another important part of brain is hypothalamus. It is very small in sie. It controls many important functions which are as follows: Blood pre
Describe the events which lead to the formation of (a) identical twins, (b) fraternal twins. (a) Identical twins are derived from the products of a one zygote which separa
• Cyclins accumulate during the G1, S, and G2 phases of the cell cycle. • By the G2 checkpoint (the red bar in the figure), enough Cyclin is available to form MPF complexes (aggre
The long term adaptive mechanisms involve myocardial hypertrophy and remodeling which occurs slowly over weeks to months. The capacity of these adaptive mechanisms meant to sust
The sequence for the Beta Actin gene (ACTB) is given below. Which pair of primers (designed to amplify the entire sequence below) could be used to successfully produce the beta act
Determine Effect of Inadequate calcium intake and iron deficiency anaemia? The requirement of iron and calcium is at its peak in adolescence due to an increase in the skeletal
Define Descending type of paper chromatography? In descending chromatography, the movement or flow of mobile phase is in downward direction. In this technique the solvent is ke
Is there a difference between something that is alive and something that is a living organism?
This problem refers to the MN and ABO loci mentioned in class. It also refers to the Rh locus, which is responsible for the positive/negative part of the blood type. The Rh+ allele
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