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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Q. What is the ecological role of earthworms? Earthworms have an important ecological role as they eat decomposing organic material. They also dig tunnels in the subsoil allowi
Define Endocrine Systems and Metabolism? As mentioned earlier, the main endocrine gland-pituitary gland secretion decreases. Another important feature in elderly is decline in
Explain Procedure for Capsule Staining in a Culture? We have learnt that Capsule staining can be undertaken either by negative staining or Anthony method. Now carry out the exe
Explain the Miracidium larva? Miracidium is the first stage larva that comes out from the fertilized egg. i) Miracidium is an oval, microscopic, flattened larva which appear
Define Potential Health benefits from resistant starch? Like dietary fibre, RS can also play a potential role in helping to maintain or improve health of an individual. As you
Determination of Limb Polarities The limb pattern is characterized by three axes (or polarities) as displayed in diagram: (1) The direction from the shoulder (or hip) to t
OXIDATION OF PYRUVATE TO ACETYL CoA The oxidation of pyruvate to acetyl CoA is the irreversible route from glycolysis to the citric acid cycle. What is citric acid cycle?
Q. Explain the Wash Technique used in sterilization process? Use of artificial nails is contraindicated as is use of colored nail polish. Nails should be short enough to allow
Whenever a pair of alleles has different alleles is there dominance between them? Not in all cases of a gene having two different alleles is the dominance complete. There are g
Define Determinants of Food Security - Public Nutrition? We learnt above that there is also a qualitative aspect to food security, which compels the perception of food not only
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