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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Q. Vitamins and minerals requirement in diabetes? The recommendation of vitamins and minerals is the same as for general population. However, lower levels of magnesium are ass
Define drawbacks for underwater weighing method? Since the major drawbacks with UWW method are that the subject needs to be totally submerged underwater and exhale all of the a
Protein Protein: The requirement of protein is increased in typhoid, as there is a massive tissue loss. Thus, the protein intake should be increased above the normal of lg/
biting mechanism in snakes
mechanisms of reciprocal inhibition
Interrupted suture technique: Synthetic braided 2 '0' sutures (ethibond) with 17mtn needles on either end and a pledget is used. Alternate green and white sutures are taken aft
Embryonic Induction and Cell Determination The cell determination or fate of embryonic cells is regulated through factors which may reside within the embryonic cells or by the
Pathophysiology of Acute Renal Failure It is usually reversible but the deviation of physiologic function can be extreme and mortality in pediatric age group remains high. Th
The stress lab must have the following equipment: 1) A mercury Blood Pressure apparatus. 2) A Bed so that the patients ECG can be recorded in the recovery period in supine po
Define Clinical Manifestations and Nutritional Problems Associated with Cancer? In the previous section we learnt that cancer results in several changes in the metabolism of ca
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