Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

What do you mean by pneumatic bones, Q What are pneumatic bones? Birds ...

Q What are pneumatic bones? Birds have lightweighted bones with internal spaces filled with air. These bones are called pneumatic bones this feature reduces the corporal densit

How many people carry the allele, Phenylketonuria is a severe form of menta...

Phenylketonuria is a severe form of mental disability caused by a homozygous recessive allele. The condition affects about 1 in 10,000 neborn Caucasians. Estimate the frequency of

Explain the types of cell reproduction, Explain the Types of cell Reproduct...

Explain the Types of cell Reproduction ? One of the major characteristics of living organisms is their ability to grow and reproduce. This is accomplished by cell division. In

Bacteria and archea, Based on the scientific name. Streptococcus agalactia...

Based on the scientific name. Streptococcus agalactiae what morphology would you expect these cells to have?

Initial phase for nutritional treatment of neuro trauma, Define Initial Pha...

Define Initial Phase for nutritional treatment of neuro trauma patients? In this, the life threatening conditions need to be controlled first.  Soon the nutritional support mu

Procedures for diagnosis - immune responses and biomarkers, Demonstration o...

Demonstration of immune responses and biomarkers: This may be done either on the basis of sero-conversion study on paired sera samples or by challenge (protection) test.

Define interaction of niacin with other nutrients, Define Interaction of ...

Define Interaction of niacin with other Nutrients? You may recall reading earlier that trypotophan present in dietary proteins is converted to niacin. There is an interdepend

What are flavonols and flavones, What are Flavonols and Flavones? These...

What are Flavonols and Flavones? These are the most widely distributed among the flavonoids. The most commonly occurring are Quercetin, Myricetin and Kaempferol. Quercetin is q

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd