Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Elaborates congenital aortic stenosis in details, Elaborates Congenital Aor...

Elaborates Congenital Aortic Stenosis in details? More common in males (4: 1). High incidence of Bicuspid Aortic valve. Murmur present from early infancy and sometimes at birth

Why physiological systems known as integrative systems, Q. What are the phy...

Q. What are the physiological systems known as integrative systems? Why is this designation justified? The integrative systems are the endocrine system and the nervous system.

How do calcium ions participate in muscle contraction, Q. How do calcium io...

Q. How do calcium ions participate in muscle contraction? Why do both muscle relaxation and muscle contraction spend energy? In the muscle cells calcium ions are stored within

What are the abnormalities of gaze, What are the Abnormalities of gaze ...

What are the Abnormalities of gaze Normal gaze is when visual axes both eyes are parallel in primary gaze. when visual axes are not parallel in primary gaze, it is abnormal ga

Morphological nature of endosperm, Morphological Nature of Endosperm ...

Morphological Nature of Endosperm The morphological nature of endosperm in angiosperms has been a subject of much discussion in evolution. The endosperm in gymnosperms is a g

Why fats have highest heat energy density, Why Fats have highest heat energ...

Why Fats have highest heat energy density? Fats have highest heat energy density of 9 Kcal/g. It is the major storage form of energy in body requiring least space and minimum w

Determine the recombination frequency of genes, Why does the recombination ...

Why does the recombination frequency of genes vary with the distance between them in the chromosome? The farther the distance among the loci of two genes in a chromosome the h

Tranquillizers - psychological drug dependence, TRANQUILLIZER S - Thes...

TRANQUILLIZER S - These are the drugs that reduce tension and anxiety, bring calmness without inducing sleep. (i) Phenothiazines - These are anti psychotic drugs.

How long is the incubation period of the hiv, How long is the incubation pe...

How long is the incubation period of the HIV? What is meant by acute AIDS? The incubation period of the HIV (the time interval among the infection and the beginning of the immu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd