Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Explain iron uptake by cells, Explain Iron Uptake by Cells? Iron partic...

Explain Iron Uptake by Cells? Iron participates in a large number of biochemical reactions. However, for iron to perform any function, it first needs to be taken up by the cell

Draw a class diagram, Draw a class diagram: You are required to provid...

Draw a class diagram: You are required to provide a practical implementation in C++ for the following class diagram. You have to provide the solution in the form of classes in

Method of cloning dna fragments into plasmids, During our discussion of rep...

During our discussion of replication origins we took a step-by-step look at the method of cloning DNA fragments into plasmids . Based on your knowledge of this discussion which

Briefly explain about semantides, Q. Briefly explain about semantides? ...

Q. Briefly explain about semantides? The information carrying molecules in plants are called semantides, and they have been recognised to be 3 kinds; deoxyribonucleic acid or D

Explain the chemical properties of monosaccharides, Explain the Chemical Pr...

Explain the Chemical Properties of Monosaccharides? As you already know the chemical properties of monosaccharides depend on the presence of the hydroxyl the aldehyde or the ke

Natural and artificial ecosystem, Natural and Artificial Ecosystem i) N...

Natural and Artificial Ecosystem i) Natural ecosystems are those which are mostly free from human disturbances, such as tropical forests, grasslands, oceans, lakes and deserts.

What do you understand by heterotrophic, What do you understand by Heterotr...

What do you understand by Heterotrophic? Organisms which are not capable of converting light into chemical energy. They should consume other organisms or material produced by o

Are nematodes triploblastic or diploblastic animals, Q. Are nematodes tripl...

Q. Are nematodes triploblastic or diploblastic animals? Just like platyhelminthes, nematodes are triploblastics that is they present three germ layers (ectoderm, endoderm and m

Biotechnology, Clonig,plasmid vectors,lambda&M13based vectors

Clonig,plasmid vectors,lambda&M13based vectors

Write the blood sugar in normal range and range in diabetes, Q. Write the b...

Q. Write the blood sugar in normal range and range in diabetes? Normal           Diabetes mellitus >126 mg / dl >200 mg / dl

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd