Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Eye ball, WALL OF EYE BALL - Outer to inner 3 layers present. 1. Scl...

WALL OF EYE BALL - Outer to inner 3 layers present. 1. Sclerotic or fibrous Tunic 2. Choroid or uvea or vescular Tunic. 3. Retina or neuro sensory tunic. Sclerotic

Categories of benthic flowering plants, Categories of benthic flowering pla...

Categories of benthic flowering plants Zone of emergent plants Consisting of plants whose roots and stems remain submerged in water and whose upper leaves and ste

Explain about the bulimia nervosa, Explain about the Bulimia Nervosa? B...

Explain about the Bulimia Nervosa? Bulimia Nervosa, as you may recall studying earlier, is a disorder characterized by episodes of binge eating or very rapid intake of large am

Bovine leukemia, B ov i n e leukemia Bovine leukemia, also known as...

B ov i n e leukemia Bovine leukemia, also known as bovine lymphosarcoma or leucosis, is a lymphoproliferative neoplastic disease of bovines. The virus belongs to genus Delt

Fats, definition and explanation of fats

definition and explanation of fats

Define fish as a rich source of protein, Define Fish as a rich source of pr...

Define Fish as a rich source of protein? The edible portion is skeletal muscles of the body. Even though the skeletal muscles of different animals are basically similar, fish s

What is the estimated percentage of water in the human body, Q. What is the...

Q. What is the estimated percentage (in mass) of water in the human body? Is this percentage expected to be larger in the adult or in the old individual? Ans. Approximatel

Composition of body fluids, Composition of Body Fluids All body fluids...

Composition of Body Fluids All body fluids - plasma, interstitial and intracellular have one common feature i.e. they are formed mostly of water. You would be amazed to know t

Evaluate what is the frequency of carriers in a tank, Zebrafish are raised ...

Zebrafish are raised in fish tanks with one family per tank. The mutation spadetail is a recessive lethal. A stock tank for spadetail was made by crossing two carriers. The fish in

Transcription of a eukaryotic protein-coding gene, 1. Describe how the vari...

1. Describe how the various molecular mechanisms act to regulate transcription of the genes within the lactose operon of E. coli . 2. Transcription of a eukaryotic protein-codi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd