Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Types of drug action, TYPES OF DRUG ACTION - 1 .       PHARMACODYNAMI...

TYPES OF DRUG ACTION - 1 .       PHARMACODYNAMIC DRUGS - These drugs modify the physiological and biochemical processes in the patient's body to eliminate the disease.

What is root cap, What is root cap? Root cap is a protective structure ...

What is root cap? Root cap is a protective structure located in the tip of the growing root. It defends the meristematic tissue of the root forming a cap that surrounds the tip

Cephalisation - metazoa, Cephalisation - Metazoa Bilateral animals whe...

Cephalisation - Metazoa Bilateral animals when creeping or swimming, have a tendency to keep the same end of the body forward and the same surface down towards the substratum.

Bacterial diseases- braxy, Braxy The causative agent of braxy is Cl. s...

Braxy The causative agent of braxy is Cl. septicum. It usually affects lambs. The agent is a normal inhabitant of soil and is frequently found in the faeces of herbivores. Bra

What is the destination of the branchial clefts in humans, What is the dest...

What is the destination of the branchial clefts in humans? In humans the branchial clefts situated in the anterior region of the pharynx (also called as pharyngeal clefts) are

Exercises and ambulation for cardiac patients, Exercises and ambulation ...

Exercises and ambulation Continue taking all exercises taught in the hospital. Steam inhalation may be continued. Walk on levelled space first, gradually increase the dista

Respiratory system in living organisms, Respiratory System in Living Organi...

Respiratory System in Living Organisms Respiration is a necessary physiological process in all living organisms by which they obtain energy for carrying out all the metabolic

Divisions of meiosis, Divisions of Meiosis: Meiosis is characterized by ...

Divisions of Meiosis: Meiosis is characterized by two nuclear divisions, meiosis I and meiosis II. • The first division is a reduction division. • The second meiotic divis

Importance of dark period, Importance of Dark Period For quite sometim...

Importance of Dark Period For quite sometime the role of light period (photoperiod) was emphasised in flowering? However, based on certain experiments it was realised that it

What is the normal duration of the menstrual cycle, What is the normal dura...

What is the normal duration of the menstrual cycle? How does the calendar contraceptive method work? The normal duration of the menstrual cycle is 28 days but it can vary betwe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd