Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Modification of carbohydrate intake for specific disorder, Define Modificat...

Define Modification of Carbohydrate Intake for Specific Disorder? In our daily diet almost 60-7096' of energy is contributed by carbohydrates. Majority of them are comprised of

Do sponges have circulatory system, Q. Do sponges have circulatory, nervous...

Q. Do sponges have circulatory, nervous and excretory systems? Sponges do not have a nervous system neither excretory system nor circulatory system.

What is pulmonary artery banding, What is Pulmonary Artery Banding ? B...

What is Pulmonary Artery Banding ? Banding of pulmonary trunk is done to reduce pulmonary flow in a baby. It is seldom done for isolated ventricular septa] defect. Multiple mu

Explain about cyanocobalamin, Explain about Cyanocobalamin Cyanocobala...

Explain about Cyanocobalamin Cyanocobalamin occurs as dark red crystals or as a crystalline hygroscopic powder of the same colour without odour and taste. Upon standing in the

Briefly describe about the micro minerals, Briefly Describe about the Micro...

Briefly Describe about the Micro Minerals? The last unit focused on the macro minerals. Now in this unit we will study about the micro minerals, namely, iron, zinc, copper, sel

Explain the the use of soy protein in food industry, The use of soy protein...

The use of soy protein concentrates in different applications in food industry is highlighted herewith. a. Bakery products Unlesss higher protein fortification leve

Is it possible x chromosome of women come from father, Is it possible that ...

Is it possible that an X chromosome of a woman can have come from her father? It isn't only possible that an X chromosome of a woman is from her father, it is certain. Each wom

Lipoidal models, LIPOIDA L MODELS According to Overton (1902) plasm...

LIPOIDA L MODELS According to Overton (1902) plasma membrane consists of single layer of lipid, because cell permeability is related to lipids. According to Gorter and

Interdependance, Give an example of how two organisms are interdependent.

Give an example of how two organisms are interdependent.

What are deciduous trees, What are deciduous trees? The Deciduous trees...

What are deciduous trees? The Deciduous trees are plants that lose their leaves in a period of the year and in the case of the deciduous trees of the temperate forest the fall

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd