Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

#title.respiratio., i want notes for respiration in humans full qand to the...

i want notes for respiration in humans full qand to the point si i can learn them

Favus, Favus Fowl favus is a superficial, chronic, sporadic mycotic in...

Favus Fowl favus is a superficial, chronic, sporadic mycotic infection of birds caused mainly by Trichophyton gallinae. Very rarely Trichophyton simii is also involved in the

Determine the atomic number of element, A.    In order to determine the ato...

A.    In order to determine the atomic number of an element we need to know the number of protons in an atom. As we know that atom is neutral we can also take the number of electro

Determine the expected genotypic and phenotypic results, 1. In guinea pigs,...

1. In guinea pigs, black coat color (B) is dominant to white (b), and a rough coat (R) is dominant to smooth (r). What are the expected genotypic and phenotypic results of the foll

Respiratory system - developmental changes, Respiratory System - Developmen...

Respiratory System - Developmental Changes after Birth The new-born's lungs are collapsed and need powerful breaths to inflate them; airways too are small, offering remarkable

Define hexose monophosphate pathway, Define hexose monophosphate pathway ...

Define hexose monophosphate pathway The hexose monophosphate pathway (HMP also called the pentose phosphate pathway, or phoshogluconate pathway) consists of Mo irreversible o

Symptoms of celiac disease, Q. Symptoms of celiac disease? The list of ...

Q. Symptoms of celiac disease? The list of symptoms as mentioned in various sources for celiac disease includes: • Digestive symptoms - diarrhoea, abdominal pain, abdominal

Texture of food product, Q. Texture of food product? Texture may be acc...

Q. Texture of food product? Texture may be accessed through touch or mouth feels. When the food is placed in the mouth, the surface of the tongue and other sensitive skin react

Why domain archaea are known to contain a true nucleus, Bacteria never have...

Bacteria never have a nucleus but some members of the domain Archaea are known to contain a true nucleus.

Define the obesity in children, Define the Obesity in Children? It is d...

Define the Obesity in Children? It is difficult to measure overweight or obesity in children and adolescents because they grow and mature at different rates. Weight status in c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd