Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

What is circulation, Q. What is circulation? Circulation is the movemen...

Q. What is circulation? Circulation is the movement of substances like gases within blood vessels and nutrients and cavities throughout the organism. Q. Do all animals have

What are the typical features of mammals, What are the typical features of ...

What are the typical features of mammals? The typical features of mammals are: body (more or less) covered with hair; presence of the diaphragm muscle (that divides the thorax

Explain wound healing and formation of woven bone, Wound healing and format...

Wound healing and formation of woven bone Stage 1: Wound healing and formation of woven bone (callus) (2 to 6 weeks) The ability of the body to respond to the "trauma" indu

Dispersal of seeds , Dispersal of Seeds A plant usually bears many fr...

Dispersal of Seeds A plant usually bears many fruits and innumerable seeds. If all the seeds produced by a plant were to germinate in the immediate vicinity, this will have s

Spinal cord sectioned at the cervical level, Q. How is it explained that a ...

Q. How is it explained that a person with the spinal cord sectioned at the cervical level is still able to perform the patellar reflex? The arch reflex depends only on the inte

What is pollen, What is pollen? Pollen grains are the male gametophytes...

What is pollen? Pollen grains are the male gametophytes of the phanerogamic (flowering) plants. Thus within the pollen grains the male gametes of these plants are produced by m

Explain some important phospholipids, Explain some important phospholipids ...

Explain some important phospholipids The chemical structure of  phosphatidylcholine (lecithin) illustrated here in Figure is  typical of  the phosphatides  found in the brai

What are the grain boundaries, What are the Grain boundaries Grain boun...

What are the Grain boundaries Grain boundaries disrupt the motion of dislocations by a material, so reducing crystallite size is a common way to improve strength. Since grain b

What concerns should alek''s healthcare, Nine-year-old Alek is admitted to ...

Nine-year-old Alek is admitted to the emergency room with a closed fracture of the right femur. Radiography revels that the fracture crosses the distal epiphyseal plate. What conce

Procedure for define percentage of reducing and total sugar, Procedure For ...

Procedure For Define Percentage of reducing and total sugar by fehling soxhlet? 1. Standard titration (Standardization of Fehling's solution) - Titrate 10 ml of Fehling's solut

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd