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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Q What are the major morphological features of birds? Birds are animals that present aerodynamic bodies covered with feathers, pneumatic bones, anterior limbs transformed into
Q. Physical Signs of aortic regurgitation? Chronic severe aortic regurgitation is characterized by wide pulse pressure and multiple peripheral signs it produces. Sharp rapid up
Genomic libraries are screened by hybridization with a DNA probe which is complementary to component of the nucleotide sequence of the desired gene. The problem should be
(a) Bedroom : · Do not place room heaters near any inflammable materials. · Do not use heaters
M a g n etic resonance imaging (MRI): It is in use as a clinical diagnostic tool since 1980. The distribution of hydrogen nuclei (protons), present in cellular wa
Explain the Steps of Exercise You have learnt the steps of exercises in Block 3, Unit 1 of theory block. Let us review the steps. In aerobic exercises one has to under go fo
Mas s Extinction - Since beginning many species evolved and got extinct (in fact only 1 % is living). Sometimes it occured suddenly at large scale, (most recently dinosa
Trout beck zone - Lotic Ecosystem This is larger and more constant than the head stream. The greater volume of torrential water carves channels into exposed rock floor (bed ro
Q. What is the protein denaturation? Is there any change in the major structure when a protein is denatured? Secondary, tertiary and quaternary structures of proteins are spati
Q. What is the average incubation period of Chagas' disease? What are some signs and symptoms of the acute phase of Chagas' disease? The Chagas' disease may or may not present
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