Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Where can rna be found within cells, Where can RNA be found within cells? ...

Where can RNA be found within cells? In the eukaryote cell nucleus RNA can be found dispersed in the nuclear fluid, with DNA, and as the main constituent of the nucleolus. In c

Best relates the severity and frequency of natural events, In general, whic...

In general, which of the following best relates the severity and frequency of natural events, such as storms or droughts? A) Nothing is known about this relationship. B) The severi

Extinction, EXTINCTIO N  - It refers to disappearance of a species from...

EXTINCTIO N  - It refers to disappearance of a species from earth when its last surviving member dies. Organic evolution not only creats new sps. but also eliminates some ol

Explain the radiographic success of root canal treatment, Explain the Radio...

Explain the Radiographic Success of Root Canal Treatment Elimination or lack of development of an area of rarefaction (= Radiolocency )  for a minimum of I year after treatment

Plants are supplied with adequate amount of phosphorus, Plants are supplied...

Plants are supplied with adequate amount of phosphorus Phosphorus has also been associated with early maturity of crops particularly the cereals. Its shortage is accompanied by

What is phytates, Q. What is Phytates? Phytates are widely distributed...

Q. What is Phytates? Phytates are widely distributed in seeds of vegetables, fruits and grains. Unrefined cereals and millets are the richest sources of phytates. Refined or

State in brief about gullstrands theory, State in brief about Gullstrands T...

State in brief about Gullstrands Theory A change in the lens is controlled by the balance between elasticity of the lens capsule and the pull of zonules. It is believed that on

What are alleles of a gene, What are alleles of a gene? Diploid individ...

What are alleles of a gene? Diploid individuals have paired chromosomes. For instance in humans there are 23 pairs of chromosomes totalling 46 chromosomes. Each pair comprehend

Adaptations to high wind velocity, Adaptations to high wind velocity Th...

Adaptations to high wind velocity The mechanical force of the wind and the grinding action of sand, dust, snow and other materials driven by it cause the plants to adapt themse

Respiration would cease even oxygen and food were supplied, If a tissue was...

If a tissue was heated to 65ºC for 10 minutes, respiration would cease even if oxygen and food were supplied. Why is this? A temperature of 65°C for 10 minutes would denature

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd