Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Microorganisms on basis of oxygen requirement for growth, Q. Microorganisms...

Q. Microorganisms on basis of oxygen requirement for growth? On basis of oxygen requirement for growth: - Obligate Aerobes: Require oxygen for growth and multiplication e.g

What is archaebacteria, What is Archaebacteria ? Archaebacteria is a re...

What is Archaebacteria ? Archaebacteria is a relatively new grouping that was created in the light of relatively recent biochemical and electron microscopic evidence. These fin

Explain pseudocoelomates in classification animal kingdom, Explain Pseudoco...

Explain Pseudocoelomates in classification animal kingdom? In terms of body organization, Pseudocoelomates fall between the Acoelomates and the Coelomates. The body cavity in

Cell biology, Explain the functis of the main organelles in plant and anima...

Explain the functis of the main organelles in plant and animal cell as seen under the lightmicroscope

Comparison between regeneration and embryonic development, Comparison betwe...

Comparison between Regeneration and Embryonic Development By now you must have realized that the procedures of regeneration and embryonic development have several fundamental

Define nutritional requirements for young and older children, Define Nutrit...

Define Nutritional requirements for Young and Older Children? Young children need 3 en%, which would be easily met from 8-10 g of oil. However, more visible oil is needed to im

What is manifest squint, What is Manifest Squint (Heterotropia) Manifes...

What is Manifest Squint (Heterotropia) Manifest squint is a deviation of one eye from the normal position when the fusion reflex is present. The subcategories of manifest squin

Define factors that are involved in the regulation, Describe the factors th...

Describe the factors that are involved in the regulation of respiration in a child who decides to hold his/her breath for as long as possible.

Physical and physiological changes - geriatric nutrition, Define Physical a...

Define Physical and Physiological Changes? Every stage has its unique requirements due to different changing needs. With respect to nutrition and health, four different basic a

What are the results of atrial septal defect, What are the results of Atria...

What are the results of Atrial Septal Defect ? Mortality for ASD closure has approached 0 per cent in most cardiac surgery centers. Late survival of patients who has ASD closu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd