Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Explain about vitamin C, Vitamin C (Ascorbic acid) Vitamin C, ascorbic ...

Vitamin C (Ascorbic acid) Vitamin C, ascorbic acid is also called anti-scorbutic vitamin. Ascorbic acid occurs as white crystals or white crystalline powder without odour and o

Temperate shrublands, These are areas where woody shrubs predominate rather...

These are areas where woody shrubs predominate rather then trees. In regions with a Mediterranean type of climate i .e., hot dry summers and cool wet winters, shrubs grow close tog

Sharps-hazards in biology laboratory, SHARPS : Cuts due to careless handli...

SHARPS : Cuts due to careless handling of sharps such as sectioning razors, microtome blades etc. are probably the most common cause of injury in the biology lab. The only real re

Define the beer''s law - nutritional biochemistry, Define the Beer's Law -...

Define the Beer's Law - Nutritional  Biochemistry? Beer's law states that the concentration of a substance is directly proportional to the amount of light absorbed or inversely

What are presbyopia and astigmatism, What are presbyopia and astigmatism? ...

What are presbyopia and astigmatism? Presbyopia is the visual impairment in which there is loss of the cililary muscle strength therefore reducing the capability of the crystal

Biochemical production , Biochemical Production: Plants are the foundat...

Biochemical Production: Plants are the foundation of a large variety of biochemical which are metabolites of both secondary and primary metabolism. But secondary metabolites ar

Explain diabetes mellitus, Diabetes mellitm This anabolic hormone exert...

Diabetes mellitm This anabolic hormone exerts its action on key glycolytic  enzymes  thus  leading  to the conversion of glucose  to  pyruvate as explained under the regulation

Monosaccharides, discuss the stereo isomerism of monosaccharides

discuss the stereo isomerism of monosaccharides

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd