Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Of what subunits are ribosomes are made, Q. Of what subunits are ribosomes ...

Q. Of what subunits are ribosomes are made? Ribosomes are made of two subunits the small subunit and the large subunit. These subunits are made of proteins and ribosomic RNA (r

Explain about central nervous system, Central Nervous System (CNS) Cove...

Central Nervous System (CNS) Covering of the brain and spinal cord is called Meanings (membranes which cover and protect brain and spinal cord are called meanings) There is flu

Define keratomalacia - micronutrient deficiencies, Define Keratomalacia - M...

Define Keratomalacia - Micronutrient Deficiencies? This is a condition of rapid destruction and liquefaction of full thickness of cornea, leading lo prolapse of iris, resulting

What is the menstrual cycle, Q. What is the menstrual cycle? The menstr...

Q. What is the menstrual cycle? The menstrual cycle is the periodic succession of interactions between the organs and hormones of the female reproductive system after the begin

Pathophysiology, Case scenario Mr Kit Lee, a 28-year-old unrestrained dr...

Case scenario Mr Kit Lee, a 28-year-old unrestrained driver of a utility, was travelling on a highway at 100kph and swerved to miss road debris. He lost control and struck a tre

What is physical activity ratio, What is Physical activity ratio? Physi...

What is Physical activity ratio? Physical activity ratio (PAR): The energy cost of an activity per unit of time (usually a minute or an hour) expressed as a multiple of BMR. It

What is biological magnification, a) What is biological magnification? ...

a) What is biological magnification? b) Describe how DDT as a water pollutant undergoes biological magnification?

Explain the periapical surgery - endodontic surgery, Explain the Periapical...

Explain the Periapical surgery - Endodontic Surgery a) Curretage 1 b) Root-end ressection 2 c) Root-end preparation 3 d) Root-end filling 4

Define tuberculosis, Define tuberculosis Tuberculosis (TB) is still a p...

Define tuberculosis Tuberculosis (TB) is still a problem in the United States, even though the incidence continues to decline in most of the country. Treatment of TB can be div

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd