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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
There are three steps of PCR a) Denaturation. The reaction combination is heated to 95°C for a short time period (about 15-30 sec) to denature the goal DNA into one strands w
Q. Write the blood sugar in normal range and range in diabetes? Normal Diabetes mellitus >126 mg / dl >200 mg / dl
Explain Magnetic Stirrer - Food Microbiology It can be used for mixing ingredients at the time of media or reagents preparation. Mixing happens with spinning of a teflon coat
what is the phylum protozoa and when he was discover
Q How do amoebae, paramecia and trichomonas respectively move? Amoebae move by amoeboid movements small invaginations and projections of their plasma membrane (pseudopods) that
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Foraminifera is the single-celled protists which secrete a shell or test. This is the accumulations of the shells of dead foraminifera and the other microscopic sea creatures form
10 organisms &there mode of nutrition
What is the relationship between the estrogen level and the LH level in the menstrual cycle? What is the function of LH in the menstrual cycle and when does its blood concentration
cutaneous respiration
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