Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

#tzoology, #affinities of ctenophora with platyhelmintheson..

#affinities of ctenophora with platyhelmintheson..

Asymptomatic patient-chronic mitral regurgitation, Asymptomatic patient: ...

Asymptomatic patient: The current opinion is that in asymptomatic patients with left ventricular dysfunction and severe MR surgery should not be delayed. The echo cardiographic a

From which structures is the nuclear membrane composed, Eukaryotic cells ha...

Eukaryotic cells have nucleus delimited by two juxtaposed membranes that start with the membrane of the endoplasmic reticulum. The nuclear membrane, or karyotheca, represents pores

Telophase, Telophase This is the final stage of mitosis  in which the m...

Telophase This is the final stage of mitosis  in which the mitotic  spindle  disappears  and  each polar  chromosomal group is reconstituted  into a daughter  nucleus,  A   nuc

How to devise a series of dilutions, A solution was prepared by dissolving ...

A solution was prepared by dissolving 25g NaOH powder in 100 ml water. Using not more than 15 ml water, devise a series of dilutions that will give a concentration of 5mg/ml NaOH.

Explain the digestibility coefficient - proteins, Explain the Digestibility...

Explain the Digestibility Coefficient? You have earlier learnt that dietary proteins are hydrolyzed to amino acids during digestion. The digestion begins in the stomach by the

Blood, How the blood is circulated with the help of Heart?

How the blood is circulated with the help of Heart?

Leaves give off water vapour, Leaves give off water vapour Use two same...

Leaves give off water vapour Use two same pots of soil, one with a small plant and the other without. Cover the soil in every pot with cardboard as shown in the diagram after w

Illustrate the respiratory system, Illustrate the Respiratory System In...

Illustrate the Respiratory System In general, respiration and breathing are understood to be same. But it is not so. Breathing simply means taking in oxygen from air and giving

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd