Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Adverse effects of noise pollution, 1.      Effect on general health:  ...

1.      Effect on general health:          Noise pollution increases the rate of heart beat, constriction of blood vessels and blood pressure. Noise also causes anxiety, stres

Explain about the physiological accommodation, Explain about the Physiologi...

Explain about the Physiological Accommodation Physiological Accommodation contractile power of the ciliary muscle required to change the focussing power of the eye by ID. Meas

Myofilaments of myofibrils, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Define th technique - stage two using a mucoperiosteal flap, Stage two usin...

Stage two using a mucoperiosteal flap A Mid crestal incision over the implant site is made and reflection of mucoperisteal flap is done under local anesthesia. After locating t

Difference between reproduction system of man and rabbit, Difference betwee...

Difference between Reproduction System of man and rabbit -   S.No.   Man   Rabbit   1.   2.   3.

Pathology of aortic regurgitation, Q. Pathology of aortic regurgitation? ...

Q. Pathology of aortic regurgitation? In Post Inflammatory situations scarring and retraction of valve cusps lead to aortic regurgitation. Varying degrees of commissural fusion

Do bacteria cells have a nucleus, Q. Do bacteria cells have a nucleus? ...

Q. Do bacteria cells have a nucleus? In bacteria the genetic material is dispersed in the cytosol and there is no inner membrane that delimits a nucleus.

Chara, Assighments of chara

Assighments of chara

Explain the adsorption or binding ability, Explain the Adsorption or Bindin...

Explain the Adsorption or Binding Ability? Some fibre components have the ability to bind (adsorb) substances in the gastrointestinal tract. Wheat bran, guar gum, mannan and is

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd