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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
During mitotic anaphase is there separation of homologous chromosomes or separation of identical chromatids? In the anaphase of mitosis the identical chromatids separate and co
Empirical validity of test procedures Reitan's great concern has always been with the empirical validity of test procedures. Such validity can only be established by the collec
You are studying a gene, and its effects on skin cells. You want to find out A) If the protein made encoded by this gene binds to DNA in a particular place B) if the protein binds
What Do We Mean By Culture Media? Culture media, you would realize, is a solid or liquid preparation containing all the nutrients required by microbes for growth. So then, what
Q What are the chloroplasts? What are the major functions of chloroplasts? Chloroplasts are organelles present in the cytoplasm of algae and plant cells, like mitochondria, chl
What is Septal Ablation (TASH/PTSMA) ? This is a nonsurgical interventional treatment. The septal myocardium supplied by 1 st septal branch of left anterior descending artery
Explain the chemical properties of milk Autoclaving milk, wherein temperature of around 121 o C is achieved, causes browning. The brown colour is due to the heat effecting an i
Explain the Principle of alkaline copper reduction method? All sugars which contain a free aldehyde group undergo enolization when placed in hot alkaline solution. This propert
GLUCOSE IS OXIDISED TO PRODUCE ENERGY TO GIVE ADP TO FORM ATP
What is the main function of chloroplasts? Ans) Chloroplasts are organelles represent in the cytoplasm of plant and algae cells. Similarly mitochondria, chloroplasts have two bo
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