Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

What are the basic constituents of the cell membrane, The cell membrane is ...

The cell membrane is produced of lipids, proteins and carbohydrates. The membrane lipids are phospholipids, a particular type of lipid to which one extremity a phosphate group i

Why is genetic homozygosity dangerous, Why is genetic homozygosity dangerou...

Why is genetic homozygosity dangerous to a nearly extinct species? Genetic homozygosity leaves no variation for natural selection to act on. Thus, a new disease could wipe

Atrial fibrillation or flutter, Q. Atrial Fibrillation or Flutter? Tran...

Q. Atrial Fibrillation or Flutter? Transient atrial fibrillation or flutter is seen frequently and can be associated with CAD, rheumatic heart disease, thyrotoxicosis, or myoc

Determine the muscular-skeletal system, Determine the Muscular-Skeletal Sys...

Determine the Muscular-Skeletal System It is known we all do movement and these take place by movement of muscles in coordination with the skeletal system. In human body muscle

What is mitosis, What is Mitosis? Mitosis is a process whereby a cell...

What is Mitosis? Mitosis is a process whereby a cell that is preparing to divide separates the duplicated pairs of chromosomes from each other so that each of the new daughte

Light stress in chloroplasts, Light Stress in Chloroplasts As mentione...

Light Stress in Chloroplasts As mentioned earlier, very high light intensity can inhibit photosynthesis due to accumulation of excess of excitation energy. A portion of this r

Determine the term poliomyelitis - brian diseases, Determine the term Polio...

Determine the term Poliomyelitis - Brian diseases Poliomyelitis is an acute infectious disease caused by a virus that has a special affinity for the motor neurons of the spinal

Explain the largest group of bacteria eubacteria, Explain the largest group...

Explain the largest group of bacteria Eubacteria? Eubacteria are the largest group of bacteria within Kingdom Monera. They are the "true" bacteria, what most people referred to

Explain doctrine of signatures, Explain Doctrine of Signatures? Handed ...

Explain Doctrine of Signatures? Handed down from the ancients, and elaborated on by the herbalists, was the 'Doctrine of Signatures', which was based on features that resembled

What is the indolacetic acid (iaa), What is the indolacetic acid (IAA)? ...

What is the indolacetic acid (IAA)? IAA or Indolacetic acid (indolyl-3-acetic acid) is the major natural auxin made by plants. It promotes plant growth and cellular differentia

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd