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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Determine SNP variation among the aligned DNAs for a genomic region. See below for how to count SNP variation. The output file (Your_name_snp.txt) should have two
If the generation time (t) is the incubation time (t) per generation (G), or t = t/G, rewrite the formula you derived in question 2 for bacterial population growth in terms of incu
Explain the Techniques of Culturing? In the last practical we learnt how to prepare culture media. This practical has been introduced to make you aware of the technique of sub-
Explain the Infarct Exclusive Technique? Infarct Exclusive Technique : Left ventriculotomy is done through the infarcted area. No attempt is made to close the ventricu
What are the major natural plant hormones and what are their respective effects? The major natural plant hormones and their respective functions are the following- Auxins that
Cancer Lung Incidence of cancer lung is more in persons who smoke. Environmental and industrial pollution is also a risk factor in the increase in incidence. Clinic
Q. A netlike membranous complex of superposed flat saccules with vesicles detaching from the extremities seen in electronic microscopy. What is the observed structure? What is its
How do the cDNAs vary from the original genes? A. The inserted cDNA copies lack enhancers, promoters and introns. B. The genomic structures of the original gene and the ins
Carbon dioxide needed for photosynthesis enters the leaf via the stomata but water is also lost when these pores are open. Water is often limiting and to limit water loss through s
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