Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Industrial melanism, In this section, we shall discuss a classic example of...

In this section, we shall discuss a classic example of natulal selection in action. In the preceding unit, it was stated that natural selection always aims at eliminating alleles,

Tissue culture, Tissue Culture It is an important technique for maintai...

Tissue Culture It is an important technique for maintaining a'pm or piece of animal or plant tissue alive after their culture dishes. It is necessary to provide an environment

Biological properties of protoplasm, BIOLOGICAL PROPERTIES OF PROTOPLASM - ...

BIOLOGICAL PROPERTIES OF PROTOPLASM - 1 .       Respiration - Oxidation of food to liberate energy is respiration. Every living beings respire but every living beings do no

What is coevolution, What is Coevolution ? There is considerable eviden...

What is Coevolution ? There is considerable evidence that supports an interesting theory that two individual species can affect each others evolution in reciprocal fashion. In

Why is it not correct to assert that dna self-replicates, Why is it not cor...

Why is it not correct to assert that DNA self-replicates? DNA is not completely autonomous in its duplication process because the replication does not happen without enzymatic

Classification of protozoa, Classification of Protozoa Traditionally, ...

Classification of Protozoa Traditionally, protozoans have been classified as flagellates, amoebae, sporozoans and ciliates. We have retained this broad grouping for convenienc

Define association between inflammation and fever, Q. What is the associati...

Q. What is the association between inflammation and fever? In the tissue region where inflammation occurs prostaglandins, cytokines, bacterial toxins, interleukins and endothel

Define premotor cortex located in the frontal lobes, Q. Define Premotor Cor...

Q. Define Premotor Cortex Located in the frontal lobes? Premotor Cortex - Involved in control of more complex, learned motor skills like writing, driving, etc. Broca's area or

Define casein - tests for presence of exoenzymatic activity, Define Casein ...

Define Casein - Tests for Presence of Exoenzymatic Activity? Casein is a major milk protein composed of various amino acids linked through peptide bonds. Extra-cellular enzyme

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd