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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Q. Pathophysiology of mitral stenosis? Normally there is no pressure gradient between left atrium and the left ventricle during diastole. However, as the valve orifice size dec
Define protein requirements of Preschoolers? For Preschoolers the protein requirements of children of various age groups are computed by the factorial method as employed by FAO
Q. Which are the major positive ions found in living beings? The major cations found in living beings are the calcium cation (Ca++), the potassium cation (K+), the sodium catio
Name few polymers in the eukoratic cell? Name the mummers that make up the DNA? Compare and contrast between mummers of DNA VS monomers of RNA?
How Phosphorus in Blood is presented? Phosphorus in Blood is Present as: 1. Inorganic phosphorus in the form of HPO 4 or H 2 PO 4 2. Organic or ester phosphorus such
Q. Classification of microorganisms? The microorganisms use our food as a source of nutrients for their own growth. This, of course can result in deterioration of the food. By
#question.sir please explain amoeba as in example of protozoa
Neural Basis of Responses to Sex Pheromones The ability of insects to detect and discriminate between a variety of pheromones with great sensitivity depends on the presence of
Energy Energy: Fever is characterized by elevation of BMR, thus caloric requirements are increased.. The increase in caloric needs is dependent on severity of infection
Culture Conditions The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of l
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