Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Explain amylose, Amylose Amylose molecules have molecular weights rangi...

Amylose Amylose molecules have molecular weights ranging from 10 4 -20 6 . Amylose can form an extended shape (hydrodynamic radius 7-22 nm) but generally tends to wind up into

Minerals requirements for ulcerative colitis, Q. Minerals requirements for ...

Q. Minerals requirements for ulcerative colitis? Minerals: Mineral losses may be marked and unless replaced may contribute to a fatal outcome. A patient with moderately advance

Determine the symptoms of bacillus cereus, Determine the Symptoms of bacill...

Determine the Symptoms of bacillus cereus Extremely large numbers (10 8 per gram) of viable cells of B. cereus must be ingested to develop signs and symptoms of the syndrome.

What do you mean by eutrophication, Q. What do you mean by eutrophication? ...

Q. What do you mean by eutrophication? The Eutrophication is the process of excessive increasing of nutrients, like nitrate and phosphate in water due to direct deposit of non

What are the in-vivo imaging techniques, What are the in-vivo imaging techn...

What are the in-vivo imaging techniques The first of the in-vivo imaging techniques, computer tomography (CT) scanning, came on stream in the early 1970s. As technologies devel

Hematology case study , would i be able to get help on these case study que...

would i be able to get help on these case study questions

Define requirements of vitamin a for infants, Define requirements of Vitami...

Define requirements of Vitamin A for infants? As for vitamin A deficiency, requirement of vitamin A is the most easy to meet as it is abundantly present in green and yellow veg

What is a photosynthesis, Photosynthesis is a process used by plants and ot...

Photosynthesis is a process used by plants and other organisms to change the light energy captured from the sun into chemical energy which can be beneficial to fuel the organism's

What is the function of the antidiuretic hormone, Q. What is the function o...

Q. What is the function of the antidiuretic hormone? Where it made and which is the stimuli that increase or reduce its secretion? The antidiuretic hormone is secreted by the h

Explain the quebec colony counter, Explain the Quebec Colony Counter? I...

Explain the Quebec Colony Counter? It is used for counting the number of colonies in a plate. Culture plate is kept on the screen which is illuminated from beneath. Counting is

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd