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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Explain Iron Uptake by Cells? Iron participates in a large number of biochemical reactions. However, for iron to perform any function, it first needs to be taken up by the cell
Draw a class diagram: You are required to provide a practical implementation in C++ for the following class diagram. You have to provide the solution in the form of classes in
During our discussion of replication origins we took a step-by-step look at the method of cloning DNA fragments into plasmids . Based on your knowledge of this discussion which
Q. Briefly explain about semantides? The information carrying molecules in plants are called semantides, and they have been recognised to be 3 kinds; deoxyribonucleic acid or D
Explain the Chemical Properties of Monosaccharides? As you already know the chemical properties of monosaccharides depend on the presence of the hydroxyl the aldehyde or the ke
Natural and Artificial Ecosystem i) Natural ecosystems are those which are mostly free from human disturbances, such as tropical forests, grasslands, oceans, lakes and deserts.
What do you understand by Heterotrophic? Organisms which are not capable of converting light into chemical energy. They should consume other organisms or material produced by o
Q. Are nematodes triploblastic or diploblastic animals? Just like platyhelminthes, nematodes are triploblastics that is they present three germ layers (ectoderm, endoderm and m
Clonig,plasmid vectors,lambda&M13based vectors
Q. Write the blood sugar in normal range and range in diabetes? Normal Diabetes mellitus >126 mg / dl >200 mg / dl
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