Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Experiment of an egg osmometer, An egg osmometer Place some dilute hydr...

An egg osmometer Place some dilute hydrochloric acid or strong vinegar in a shallow dish, likeas a saucer, to a depth of about one centimetre. Hold the large end an egg in the

What is interdental threaded cleaners, What is Interdental Threaded Cleaner...

What is Interdental Threaded Cleaners The use of floss is recommended around any implant-supported bridge, crown, or bar. Instruct the patient to wrap the floss around the impl

Determine what is the Cell Cycle, The cell cycle undergoes a sequence of ch...

The cell cycle undergoes a sequence of changes which involve a period of growth replication of DNA, Followed by cell division. This sequence of changes is called cell cycle.

What are the allele frequencies, You observe the following numbers of indiv...

You observe the following numbers of individuals of each genotype in a population: AA 700 Aa 200 aa 100 a.) what are the observed genotype frequencies in this populaiton?

Hypertension or blood pressure, Hypertension is one of the commonest diseas...

Hypertension is one of the commonest disease states encountered in medical practice. It is  one of the simplest to diagnose and to treat if one follows certain guidelines which are

Define oven - nutritional biochemistry, Define Oven - Nutritional Biochemis...

Define Oven - Nutritional Biochemistry? Oven is a general term used to describe a chamber or enclosed compartment for heating, baking, or roasting food, as in a stove, or for f

Whta is the effect of processing on mineral content of foods, Effect of pro...

Effect of processing on mineral content of foods Minerals are comparatively stable under processing conditions such as heat, light, use of oxidizing agents and extremes in pH.

Studying leaf arrangements, Studying leaf arrangements Observe as many ...

Studying leaf arrangements Observe as many growing plants as possible by looking down on them from above. Draw sketches of the dissimilar patterns of leaf arrangement.

Define effect on oestrogens and progesterone in pregnancy, Define effect on...

Define effect on Oestrogens and progesterone in pregnancy? Oestrogens and progesterone are secreted by the placenta from 8-10 weeks of gestation. Progesterone increases through

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd