Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Food-borne clostridium, Food-borne Clostridium Clostridium botulinum a...

Food-borne Clostridium Clostridium botulinum and Cl. perfringens, the two members of the group have been implicated in food-borne illnesses. C. botulinum causes classical food

Phylum platyhelminyhes, general classification of phylum platy helminthes

general classification of phylum platy helminthes

Explain the obligatory losses of nutrients, Explain the Obligatory Losses o...

Explain the Obligatory Losses of Nutrients? Obligatory losses of nutrient are defined as 'the losses that occur when an individual is put on a diet free of that nutrient'. For

How cleaning agents works, Q. How Cleaning agents works? Food particles...

Q. How Cleaning agents works? Food particles and other debris provide good nutrients for the nlicroorganisms to grow. In fact, the food particles protect microorganisms d

Muscular force, 2.?In order to increase muscular force production, muscular...

2.?In order to increase muscular force production, muscular contractions may be modulated in two ways. Explain.

Types of nursing units, TYPES OF NURSING UNITS: The design of nursing ...

TYPES OF NURSING UNITS: The design of nursing unit should maximize the function of the unit by following its task. The design of nursing unit has also under gone a radical cha

What phenotypic ratios of the diploid, A wild-type strain of haploid yeast ...

A wild-type strain of haploid yeast is crossed to a mutant haploid strain to make a diploid. What phenotypic ratios will be observed in the haploid progeny of the diploid?

Eucaryotic cell structure , Eucaryotic cell structure All eucaryotic c...

Eucaryotic cell structure All eucaryotic cells have a cytoskeleton made up of a network of protein filaments (Figure shown below). This network gives the cell its shape, capac

How is cancer usually treated, Q. How is cancer usually treated? If the...

Q. How is cancer usually treated? If the cancer is in its initial stage cure is often done by surgical removal of the neoplastic tissue. The Cancers already disseminated are of

ATP FORMATION, what compound is phosphorylated for ATP? what is the resulti...

what compound is phosphorylated for ATP? what is the resulting compound when ATP releases energy

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd