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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Why do the sons of a haemophiliac father never vary from this trait? Why is the cleavage in mammals referred as simple homoplastic?
Precautions for determination of iodine number of lipids? 1. If using the pipette for transferring the oil, allow the pipette to drain slowly and completely, as oil takes time
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What is the difference between octopuses and mussels regarding their circulatory systems? How does that difference influence the mobility of these animals? Cephalopod molluscs,
A "phosphodiester bond" links together: Answer the 3' hydroxyl group and the N9 atom of adenine in ATP. The Phosphate group and N9 atom of adenine in ATP. Sequential nucleosides in
Q. What are taenias? What are the diseases caused by them? The Taenias, as well know as tapeworms, are platyhelminth animals (flatworms). The major diseases caused by taenias a
EXCRETIO N IN AMOEBA - NH 3 is excreted out through plasmalemma. Osmoregulation takes place by contractile vacoule, generally one, towards posterior end, contractile in
Role of Nerves - Regeneration It has been seen that soon after amputation nerves invade the regeneration blastema. If the stump is denervated by cutting the nerves supplying
Neo-zoonoses In recent times, some of the pre-existing low profile and less frequent zoonoses and some entirely newly recognized zoonoses are emerging with a new dimension. Th
What is a membrane? Membrane is any delicate sheet that divides one region from another blocking or permitting (selectively or completely) the passage of substances. The skin,
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