Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Explain zanamivir, Zanamivir  (Relenza) Started within 2 days after on...

Zanamivir  (Relenza) Started within 2 days after onset of symptoms, this orally inhaled neuraminidase inhibitor can shorten the duration of illness and may decrease the incide

Define biomaterial and an implant and state, Q. Define biomaterial and an i...

Q. Define biomaterial and an implant and state? a) Biologically compatibility: an ideal implant material will elicit mainly physiological reactions within the surrounding tissu

Explain economic status of diet, Economic Status Economic Status  :  I...

Economic Status Economic Status  :  It is one of the important practical considerations to be kept in mind while  formulating  a diet prescription. During  an acute illness, a

Two chromosomes in cell have identical length, Two chromosomes in a cell ha...

Two chromosomes in a cell have identical length and centromere position yet are not homologous, what is different about them.

What are the epithelial tissues, Q. What are the epithelial tissues? What i...

Q. What are the epithelial tissues? What is their general function and how is that function associated to the features of the tissue? Epithelial tissues also called as epitheli

Explain about bequest value of biodiversity, Q. Explain about Bequest value...

Q. Explain about Bequest value of biodiversity? Sometimes people derive satisfaction from the fact that conserved biodiversity may benefit other individuals in the future, givi

How tyndallisation used for sterilization, Q. How Tyndallisation used for s...

Q. How Tyndallisation used for sterilization? John Tyndall devised a process of sterilisation by steaming for a few minutes at 100 o C on 3-4 successive operations separated by

Reaction of the futile cycle, Reaction of the futile cycle:- A) An  ade...

Reaction of the futile cycle:- A) An  adequate level of  cAMP  stimulates formation of  the  inactive  'D'  form  of glycogen synthase and the active form of phosphorylase. Thu

Explain lipid formulations, Explain Lipid Formulations Three lipid form...

Explain Lipid Formulations Three lipid formulations of amphotericin B are marketed in the US: amphotericin B lipid complex (ABLC, Abelcet), liposomal amphotericin B (L-AmB, AmB

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd