Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Determine the membrane voltage of neuron b, Consider Neuron B in the frog c...

Consider Neuron B in the frog central nervous system whose plasma membrane has a previously unknown channel that is selectively conductive to a newly discovered tetravalent anion n

Pollinia and massulae, Pollinia and Massulae In most cases the pollen ...

Pollinia and Massulae In most cases the pollen grains of each tetrad become separated from one another and they lie freely in the pollen sac. In some plants, mostly members of

What is the principle of the design, In designing an experiment to find out...

In designing an experiment to find out whether light is required for photosynthesis    (a) what is the principle of the design    (b) what control would you use?

Thermal relations, Normal 0 false false false EN-IN X...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

How bisphosphate levels will be elevated in the liver, Following a meal, fr...

Following a meal, fructose 2,6 bisphosphate levels will be elevated in the liver. Under these metabolic conditions, all of the following enzymes will be active except: -PFK1

Chest drainage tubes (24-72 hours), Chest Drainage Tubes (24-72 hours) ...

Chest Drainage Tubes (24-72 hours) Continue milking of chest tube hourly.  Monitor the amount, colour and record on the flow chart.  Assess dressing for any soak

Types of asexual reproduction, TYPES OF ASEXUAL REPRODUCTION ...

TYPES OF ASEXUAL REPRODUCTION 1 . Binar y fission - Amoeba 2. Multipl e fission -

Antimicrobial prophylaxis - controversial, Antimicrobial Prophylaxis - Cont...

Antimicrobial Prophylaxis - Controversial The need for prophylaxis in breast surgery, herniorraphy and other "clean" surgical procedures has been controversial. Medical Letter

Define requirements of zinc during pregnancy period, Define requirements of...

Define requirements of Zinc during pregnancy period? Zinc, as a mineral, was found to be associated with foetal growth and birth weight. Low plasma zinc was associated with mor

Normality, what is the normality law?

what is the normality law?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd