Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Blood from a donor is sterile and stored in a sealed bag, Blood from a dono...

Blood from a donor is sterile and stored in a sealed bag, but it is still kept at 4°C. What is the advantage of keeping it at this low temperature?   At 4 °C, enzyme activi

Llustrate the role of each area in the evolution of the hebe, llustrate the...

llustrate the role of each area in the evolution of the Hebe Uses appropriate examples from the Hebe data to illustrate the role of each area/concept in the evolution of the He

Explain the term - social attention and environmental influe, Explain the t...

Explain the term - Social Attention and Environmental Influences The importance of social environment and early experiences in neurodevelopment is increasingly recognised. Due

Denitrifying bacteria play in the nitrogen cycle, What part do (a) nitrifyi...

What part do (a) nitrifying, (b) nitrogen-fixing and (c) denitrifying bacteria play in the nitrogen cycle? a) Nitrifying bacteria in the soil change ammonia and other nitrogeno

Ilustrate the binding of atp to actin, Which of the following is true in a ...

Which of the following is true in a skeletal muscle? A. Movement of the cross-bridge occurs only when the myosin head is detached from the actin molecule. B. The binding of

What do you mean by syphilis, Q. What do you mean by syphilis? The Syph...

Q. What do you mean by syphilis? The Syphilis, as well known as lues, is a disease caused by the bacteria Treponema pallidum. Before the invention of penicillin, syphilis was a

How different are fecundation in osteichthyes, How different are fecundatio...

How different are fecundation in osteichthyes and in chondrichthyes? In chondrichthyes fecundation is internal by means of copulation. In osteichthyes fecundation generally is

What are alleles of a gene, What are alleles of a gene? Diploid individ...

What are alleles of a gene? Diploid individuals have paired chromosomes. For instance in humans there are 23 pairs of chromosomes totalling 46 chromosomes. Each pair comprehend

What is cost effective analysis, What is Cost Effective Analysis? Cost ...

What is Cost Effective Analysis? Cost effective analysis: Cost effective analysis provides for the choice of least expensive strategy at the least cost. Here the outcome is mea

Factors influencing rs content -amylose or amylopectiz ratio, Explain the F...

Explain the Factors Influencing RS Content -Amylose or Amylopectiz ratio? Amylose/Amylopectirz ratio: In general, foods containing high amylose/amylopectin ratio lead to higher

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd