Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Digestion and feeding mechanism, state 5 notification and feeding habits in...

state 5 notification and feeding habits in some animals

Parasite and host during incubation period, Parasite and host during incuba...

Parasite and host during incubation period Incubation period is the second stage in the process of a disease. It is the time between the day of infection to the appearanc

Explain the executive functions - psychological tests, Explain the Executiv...

Explain the Executive Functions - Psychological tests Executive functions are those involved in the planning, organisation, regulation and monitoring of goal directed behaviour

Change in structure of a protein, Q. Is it expected that a change in the pr...

Q. Is it expected that a change in the primary, in the secondary or in the tertiary structure of a protein will produce furthermore functional consequences? Any change of the p

Explain vitamin a deficiency - micronutrient deficiencies, Explain Vitamin ...

Explain Vitamin A Deficiency - Micronutrient Deficiencies? Vitamin A deficiency (VAD) is a major public health problem, and the most vulnerable are preschool children and pregn

Apomixis, Apomixis The formation of sporophyte from the gametophyte w...

Apomixis The formation of sporophyte from the gametophyte without sexual process signifies apomixis. It relates to the replacement of alternation of a reduced gametophyte and

Assessment of wilm tumour, Assessment   The child with wilm's tumour wi...

Assessment   The child with wilm's tumour will present you with a large mass in the loin or an enlarging abdomen. This mass may be noticed accidently by  the parent of  the chi

Vacuoles, VACUOLES They are of 4 types - 1.       Sap Vacuole - ...

VACUOLES They are of 4 types - 1.       Sap Vacuole - Outer membrane of sap vacuole is called T onoplast . Sap vacuole contains amino sugars, carotenoids, proteins, m

Explain some medical considerations during breastfeeding, Explain some Medi...

Explain some Medical Considerations during Breastfeeding? Let us look into some special concerns during breastfeeding that might be useful to you as a dietitian. Medical Con

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd