Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

What happens to the movement of molecules at equilibrium, What happens to t...

What happens to the movement of molecules at equilibrium? At equilibrium, the movement of molecules continues, but because there is no concentration gradient, there is no net m

Integral membrane protein movement and distribution, Various proteins are f...

Various proteins are free to take a move laterally in the plane of the bilayer. One experiment used to represent this included fusing cultured mouse cells with human cells under ap

What are some examples of biological activities, What are some examples of ...

What are some examples of biological activities in which osmosis plays an important role? Hemolysis (destruction of red blood cells) by entrance of water, the hydric regulation

Role of chemical factors in controlling apical dominance, Role of Chemical ...

Role of Chemical Factors in Controlling Apical Dominance There were indications about the existence of plant hormones in the last part of 19th century. The plant hormones had

What is the large size of cephalopods, Q How is the large size of some ceph...

Q How is the large size of some cephalopods related to the kind of circulatory system they present? In cephalopods the circulatory system is closed and this provides more press

Explain about spring model, Q. Explain about Spring Model ? The spring ...

Q. Explain about Spring Model ? The spring model depicts elasticity. A force is applied in terms of load onto the spring as illustrated in the figure 8.6. When the force is app

State the swinging flashlight test, State the Swinging Flashlight Test ...

State the Swinging Flashlight Test The patient looks into the distance while the examiner shines a bright light first into one eye for a few seconds and then the other. As the

Medical and nutritional management for pectic ulcer, Q. Medical and Nutriti...

Q. Medical and Nutritional Management for pectic ulcer? To provide physiological rest and support tissue healing, treatment should be based on providing rest to the affected ar

Explain about the bioavailability of minerals, Explain about the Bioavailab...

Explain about the Bioavailability of Minerals? Polyphenols can form complexes with metal cations through their carboxylic or hydroxylic groups, and thus interfere with the inte

Explain separation of homologous chromosomes, Q. What are the respective fu...

Q. What are the respective functions of the separation of homologous chromosomes and of the separation of alike chromatids in meiosis? The separation of homologous chromosomes

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd