Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

What are the two groups of defense mechanisms of the body, What are the two...

What are the two groups of defense mechanisms of the body against foreign or harmful agents? What is the difference between them? The body has many defense mechanisms against f

Right atrial pressure, RAP can be determined semiquantitatively by assessi...

RAP can be determined semiquantitatively by assessing collapse of the inferior vena cava during inspiration. The inferior vena cava is imaged subcostally and its diameter is exa

Mitochondria, Mitochondria Mitochondria are commonly known as the "pow...

Mitochondria Mitochondria are commonly known as the "power house" of cells. These are the distinct organelles of all eucaryotic cells, plants, fungi and some protists. In the

Preparation - culture medium, Preparation - Culture Medium Now that ...

Preparation - Culture Medium Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available

Explain the sticky films - food microbiology, Explain the Sticky Films - Fo...

Explain the Sticky Films - Food Microbiology? Sticky films or tape are pressed against the surface to be assessed. Exposed film/tape is then pressed on the agar plate and analy

Experiment to test your hypothesis, Deinococcus radiodurans is a bacterium ...

Deinococcus radiodurans is a bacterium that was isolated from cooling ponds in and around nuclear power plants. It is highly resistant to ionizing radiation. Propose an hypothesis

Spermatogonia - spermatozoa, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4 Spermatogonia - Spe

Carbohydrate case study, CT is a 3 year old female who develops signs of hy...

CT is a 3 year old female who develops signs of hypoglycemia in the early morning unless she is fed during the night.  CT eats balanced meals for her age group during the day, with

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd