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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Define Goals of National and International Requirement Estimates and RDAs? One of the goals of the RDAs has been reiterated through this unit. They serve as the guidelines for
Phosphofiuctokinase-I Phosphofiuctokinase-I is activated by AMP and inhibited by ATP and citrate. When ATP is utilized in energy requiring process, the concentration ofAMP
Explain the Catabolic Responses? Hormonal responses during the hyper inetabolic phase of infection are same as in case of injury. Serum cortisol levels are elevated, glycogen i
Production of haploids by tissue culture,
DNA Replication Deoxyribonucleic acid (DNA) is the carrier of genetic data for all living creatures. An organism's genome, made of DNA, encodes the genetic blueprint for buildi
West Nile virus infection West Nile virus (WNV) is a type strain of flaviviruses and is related to Japanese encephalitis group. The virus was first isolated from a woman in the
Congenital Discrete Subvalver Stenosis : The abstraction could be fibrous or fibro muscular. It could be anywhere from just below the aortic cusps upto the level of the free edge
In a properly functioning negative feedback system A. value of the controlled variable will always be very close to the threshold value when the system is in steady state.
What is Periotest Periotest: An electronic device originally developed by Schulte designed to measure the dampening effect or attenuation degree characteristics of the peri
What are the main proteins that constitute the sarcomere? What is the function of those molecules in the muscle cells? In the sarcomere there are organized myosin and actin blo
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