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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Q. What is Galactosemia? Galactosemia is a genetic disorder caused by deficient functioning of any of these three enzymes namely galactokinase, galactose -1 - phosphate uridyl
Signal transduction occurs when an extracellular signaling molecule activates a cell surface receptor. In words, this receptor change intracellular molecules changes a response. Th
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Explain Development of excretory system? The filtration rate of kidney is low and infants find it difficult to eliminate high concentration of solutes. However, by the end of t
Viridans Streptococci These streptococci, which cause 30 to 65 per cent of NVE case unrelated to drug abuse, are normal inhabitants of the oropharynx, characteristically produce
Use of Spectinomycin in Pregnancy Spectinomycin (Trobicin) can be used to treat pregnant women allergic to beta-lactam antibiotics, but is unreliable against pharyngeal gonoco
Germplasm Conservation - plant tissue and organ culture Totipotent plant cells and shoot tips can be freeze-preserved in liquid nitrogen (-196? C) for long periods, and wh
Describe Fourth Heart Sound in details? S 4 is generated during the atrial-filling phase. It is a low frequency event. LVS, is best heard over apex. Mechanism of S 4 generat
Explain Fibre and Cardiovascular Disease (CVD)? The role-of dietary fibre in modulation of blood lipids was demonstrated by Keys and his co-workers in a series of experiments c
Q. Why is the endocrine system considered one of the integrative systems of the body? What is the other physiological system that also has this function? The endocrine system i
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