Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Ribosome in the translation of one mrna molecule, Q. How many of the same p...

Q. How many of the same proteins are made at the same time by each ribosome in the translation of one mRNA molecule? How does successive protein production occur in translation?

Explain transfer of culture from one liquid media to another, Explain Trans...

Explain Transfer of Culture from One Liquid Media to another Liquid Media? 1. Inoculating loop is to be held in the right hand. It is sterilized by incineration in the inner bl

Determine the term - chlorine, Determine the term - Chlorine   The appl...

Determine the term - Chlorine   The application of chlorides results in leaves of greater spread, a lighter green colour and with a smoother surface. However, the combustibilit

What do you determine by mantle, What do you determine by Mantle? A thi...

What do you determine by Mantle? A thin sheetlike membranous extension of visceral mass of molluscs which forms two flaps of skin. Mantle secretes the shell on the dorsal side

Genetics, discuss chromosomal theory of heredity.

discuss chromosomal theory of heredity.

Explain herbicide tolerant crops, Explain herbicide tolerant  crops C...

Explain herbicide tolerant  crops Candidates who reached the scholarship standard demonstrated a wide general knowledge of the living  world to which they could relate biologi

Stems transport liquids, Stems transport liquids (a)  Cut about 2 cm fr...

Stems transport liquids (a)  Cut about 2 cm from the end of stems of celery and place them in cold water for about an hour to freshen. Next place the stems in dishes having red

Determine the excitation threshold concerning neurons, How different are th...

How different are the concepts of action potential, resting potential and excitation threshold concerning neurons? Action potential is the maximum positive voltage level achiev

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd