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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Q. Describe Ecosystem Diversity? Ecosystem diversity means the variation between different types of ecosystems. Different species of animals, plants and micro-organisms interac
Explain the Direct Microscopic Counts (DMC) The process includes making a smear of food sample on a microscope slide followed by staining with an appropriate dye and viewing an
Define reagent required and methodology for Barfoed Test? Reagents - Solutions of glucose, fructose, galactose, lactose, maltose, sucrose and starch - Barfoed's reagent
What is the Echocardiogram ? When doubts persist whether a patient has CHD or not despite a thorough clinical exam and chest X-ray, ECG, and hyperoxia test, an echocardiogram s
What is tuberculosis? How is the disease transmitted? Is there treatment for tuberculosis? Tuberculosis is a disease caused by the Mycobacterium tuberculosis, bacteria which at
Question 1 List the cells and organs involved in immune system. Explain the role of thymus in development of T cells. Add a note on recognition of self antigens by immune system
Single-cell organisms, protists, have always been a problem for taxonomists who have trouble describing categories for organisms. Their mixed feeding habits that include being capa
Q. Explain nutrient requirements during hypertension? In order to meet the above objectives, we need to understand the nutrient requirements during hypertension. Let us start w
Tongue grafting Top of the stock is cut diagonally and in an upward direction. Scion is cut diagonally in downward direction. A second cut is given from above downwards,
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