Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Taxonomy, A In taxonomy, what is a "KEY" ? List the different types of key...

A In taxonomy, what is a "KEY" ? List the different types of keys. How are they prepared and what are they used for ?sk question #Minimum 100 words accepted#

Explain the periodic table of the elements, Explain the periodic table of t...

Explain the periodic table of the elements ? Periodic Table of the Elements :  An element is a pure substance composed of atoms all of a single kind. It cannot be broken down

Explain the theory of law of minimum, Explain the theory of Law of Minimum ...

Explain the theory of Law of Minimum    This is one of the earliest hypotheses put forward by von Liebig on the relationship between the amount of plant  nutrient in the soil a

Define fat requirement for cancer patients, Define Fat requirement for canc...

Define Fat requirement for cancer patients? During cancer there is enhanced mobilization of free fatty acids from adipose tissues resulting in subsequent depletion of total bod

Phylum protozoa, classification of organisms in phylum protozoa according t...

classification of organisms in phylum protozoa according to their class,order,family,genus,species.

Risk analysis - in-vitro studies, In-vitro studies Mechanistic  data mi...

In-vitro studies Mechanistic  data might be  supplemented by  data  from in-vitro  studies, like  as information on genotoxicity derived from reversion assays or other same ass

Explain amylose, Amylose Amylose molecules have molecular weights rangi...

Amylose Amylose molecules have molecular weights ranging from 10 4 -20 6 . Amylose can form an extended shape (hydrodynamic radius 7-22 nm) but generally tends to wind up into

Explain ophthalmic, Ophthalmic Data are limited on the effectiveness of...

Ophthalmic Data are limited on the effectiveness of antimicrobial prophylaxis for ophthalmic surgery, but postoperative endophthalmitis can be devastating. Most ophthalmologist

Explain about manual and automatic toothbrushes, Manual and Automatic Tooth...

Manual and Automatic Toothbrushes Brushing is imperative and soft or medium toothbrushes are recommended. Small heads are useful because they allow better access. Automatic too

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd