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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Reaction specificity Some enzymes catalyze only one reaction acting on a specific substrate. Example: urease and catalase acts only on urea and hydrogen peroxide,
Explain the Skin fold method to measure body fats? The skin fold method utilizes predication of body fat from sum of three or four skin folds (at biceps, triceps, sub scapular,
What is the life cycle of the gymnosperms? As all plants they show a diplobiontic life cycle, i.e., alternation of generations with diploid and haploid stages. The lasting (fin
what is tissue
Define Amino acid requirements for Human? Data regarding the essential amino acid requirements of infants, children and adults are given in terms of egg protein and cow's milk
What do you understand by the terms retrogradation and gelatinization? The realignment of the amylose and amylopectin and swollen starch granules to form a pocket is termed
What is Chroma in soil Chroma is the relative purity or strength of the spectral colour and its numerical value extend from (/0) for neutral colours to (/8) for the strongest e
Explain the Concept of Menu Planning? You have learnt from the previous units that our body's many functions, be it physical activity, mental exercise, growth (in the case of c
What are the similarities between chlorplasts and prokaryotic cells?
You are studying how proteins are targeted to the mitochondria. MIP1 is in the mitochondrial inner membrane, whereas MIP2 is targeted to the matrix. You are most interested in how
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