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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
COMPOSITION Proteins = 44-76%, Lipids = 20-53%, Carbohydrates = 1-8%, (Protein-Lipid ratio = 0.8 : 1 to 4 : 1) Most of lipid are "Phospholipids" which are amphipat
Anomalies Related to Oesophagus and Stomach Let us discuss briefly the anomalies of oesophagus and stomach. These are summarised as follows: Associated Problems 1) P
Question 1 List various methods used for determination of blood glucose. Explain the principle of each test. Add a note on advantages and disadvantages of each method Qu
Any elevation in BP is accompanied by increased risk of cardiovascular and cerebrovascular events and renal damage. The most distinctive pathological consequence
Electron acceptor is a molecule which forms the part of the electron transport system which transfers electrons ejected by chlorophyll during the process of photosynthesis. Part o
Name the hormones produced by (a) the testes, (b) the ovaries. (a) The testes produce testosterone. (b) The ovaries produce oestrogen and progesterone.
Shapes of viruses Viruses occur in a wide variety of shapes. Some are spherical or polyhedral . Some are helical appearing cylindrical or rod-like and other are complex. Virus
Q. Is it expected that a change in the primary, in the secondary or in the tertiary structure of a protein will produce furthermore functional consequences? Any change of the p
What is the difference between spermatogonium and spermatocyte I? The male germ cells are the spermatogonia (diploid cells, 2n) located in the testicles. They mature and by mea
classification
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