Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

What is genomics , We are living in an unprecedented age of biological ...

We are living in an unprecedented age of biological discovery and the application of biological knowledge.   Programmed DNA sequencing delivered, in the year of  2001 over the 2.6

Define the operations of a public nutritionist, Define the Operations of a ...

Define the Operations of a Public Nutritionist? A public nutritionist can perform the following: In the hospital-based set up, she is a part of the team delivering thera

Secondary prevention - rheumatic fever, Benzathine penicillin given every t...

Benzathine penicillin given every three weeks as IM injection (1.2 mega units) has given the best results. Oral agents (penicillin V) are given in patients with lower risk of rheum

St depression only on recovery, Q. ST Depression only on Recovery? Alth...

Q. ST Depression only on Recovery? Although ST depression during exercise often persists into recovery, it may not manifest until exercise has been terminated. The reasons for

How enzyme to cut two pieces of dna, Critical Thinking Why is it necessary ...

Critical Thinking Why is it necessary to use the same restriction enzyme to cut two pieces of DNA that are to be joined together? As each restriction enzyme recognizes a specif

Determine number of sucrose molecules in the solution, Determine number of ...

Determine number of sucrose molecules in the solution When a solid is dissolved in a volatile solvent the vapour pressure of the solution is less than the vapour pressure of th

Describe the factors of biological evolution of humans, Although there are ...

Although there are differences in interpretation of the evidence, it is generally accepted that human biological evolution has not been a linear progression from one species to the

Objection, i have question that all scientist agree that the basic unit off...

i have question that all scientist agree that the basic unit off life is a cell and scientist said this to be a cell theory... and they also said that if a theory survive continuou

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd