Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

What is passive transport, What is Passive Transport? Passive Transpor...

What is Passive Transport? Passive Transport :  Specialized proteins called permeases also move specific substances across the cell membrane in a process called carrier transp

Ion channel-linked receptors, Ion channel-linked receptors will transmitter...

Ion channel-linked receptors will transmitter-gated ion channels or ionotrophic receptors that   are included in the rapid synaptic signaling among electrically excitable cells. No

Explain counseling children, Counseling children Assess the child's sta...

Counseling children Assess the child's stage of development. Adjust counseling for a child's  dependency needs,  lack of  experience and  the development tasks faced by  him

What are the healing events around implants, Healing Events around Implants...

Healing Events around Implants Healing of an implant is compared with the processes in normal bone healing or bone regeneration and with stable osteosynthesis.

Do protozoans have asexual or sexual reproduction, Q. Do protozoans have as...

Q. Do protozoans have asexual or sexual reproduction ? In protozoans reproduction is asexual or sexual. The most frequent form of sexual reproduction is binary scissiparity or d

Patterns of metamorphosis, Patterns of Metamorphosis The pattern of me...

Patterns of Metamorphosis The pattern of metamorphosis is not the same in all insects and so metamorphosis can be broadly classified into 3 types: (1) Complete metamorphosi

Structure of hiv, Structure of HIV HIV is a spherical enveloped virus o...

Structure of HIV HIV is a spherical enveloped virus of about 90-120 nm diameter .In nucleocapsid is icosahedral. Its genome consists of a single stranded RNA filament segmented

Define functional role of sugars in food, Define Functional Role of Sugars ...

Define Functional Role of Sugars in Food? From the consumer's point of view, sugars are basically related with sweetness. Sweetness is essentially a physiological sensation. Th

Genetic material, Assuming that variability of populations were non-genetic...

Assuming that variability of populations were non-genetic, that is, not controlled by genetic material, once again chance events alone would determine which of the organisms would

Nursing care and treatment for cleft palate, Nursing Care and Treatment  ...

Nursing Care and Treatment   The only treatment is surgical repair and child may be operated before 18 months as per the choice of surgeon so  that speech pattern of child may

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd