Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Explain procedure for performing the completed coliform test, Explain Proce...

Explain Procedure for Performing the Completed Coliform Test? Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise f

EMBRYOLOGY., WHAT ARE THE TYPES OF EGG ON THE BASIS OF YOLK ?

WHAT ARE THE TYPES OF EGG ON THE BASIS OF YOLK ?

Define direct measurement of individual''s work capacity, Define Direct mea...

Define Direct measurement of individual's Work Capacity? Direct measurement of VO 2 max is determined by having the subject perform exercise at increased loads, for 12 to 15 m

Enzymes, what is the composition of enzymes ?

what is the composition of enzymes ?

Does peptide bonds are amide linkages, Which of the following statements ab...

Which of the following statements about peptide bonds are true? Peptide bonds are amide linkages Peptide bonds form from nucleophillic attack by an electron pair on an alpha-

Bovine rotavirus diarrhoea, B o v i ne rotavirus diarrhoea The bovi...

B o v i ne rotavirus diarrhoea The bovine rotavirus is a RNA virus with 11 segments of double stranded RNA belonging to the genus Rotavirus in the family Reoviridae. Rotavi

Father, who is the father of science

who is the father of science

How do mineral salts participate in osmotic regulation, How do mineral salt...

How do mineral salts participate in osmotic regulation? Osmotic pressure depends on the number of particles dissolved in a solution and not on the nature of such particles. Min

Autoregulation of coronary blood flow, The ability to maintain myocardial p...

The ability to maintain myocardial perfusion at constant levels in the face of changing driving presence is termed autoregulation. In normal cases, autoregulation is maintained at

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd