Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Name the corrosion resistant metals, Name the corrosion resistant metals ...

Name the corrosion resistant metals Most of the corrosion resistant metals or alloys used for implants have a layer of oxides on their surface. The thin (approximately 5 nm) la

What is phellogen, What is phellogen? What is its function? Phellogen, ...

What is phellogen? What is its function? Phellogen, also called as cork cambium, is the meristematic plant tissue responsible for the formation of the periderm (the covering of

Define the effect of niacin deficiency on skin, Define the effect of niacin...

Define the effect of niacin deficiency on Skin? Dermatitis is the characteristic feature of the' disease, It is symmetrical in distribution. In early stages, a bright red eryt

Define reagents required and methodology for mucic acid test, Define Reagen...

Define Reagents Required and Methodology for Mucic Acid Test? -   Sugar solution -   Conc. Nitric acid Methodology Take about 50 mg of sugar in a test tube. Add 1 ml

Vivipary - development biology, Vivipary - Development Biology In flow...

Vivipary - Development Biology In flowering plants the seed or fruit generally is dispersed and germination occurs when the conditions are congenial for growth. However, in so

Ecology, what is the importance to study the tolerance range in real-life...

what is the importance to study the tolerance range in real-life situations?

Molecular bio, he recombinant plasmid extracted from the surviving colonies...

he recombinant plasmid extracted from the surviving colonies contains a gene called SUC2 that codes for convertase, an enzyme needed to metabolize sucrose. Mutations in the SNF gen

Studying life histories of insects, Studying life histories of insects ...

Studying life histories of insects Cut large rectangular holes in the sides of a big cardboard container and cover them with muslin folded over at the edges and gummed or paste

Protozoa, general charactres and classfication

general charactres and classfication

What is dengue, What is dengue? Dengue, or dengue fever, is an epidemic...

What is dengue? Dengue, or dengue fever, is an epidemic disease in some countries (for instance, in Brazil), and its most dangerous form is hemorrhagic dengue. It is caused by

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd