Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

List the requirements of implant materials, List the requirements of implan...

List the requirements of implant materials. a) Biologically compatibility: an ideal implant material will elicit mainly physiological reactions within the surrounding tissues (

Define the estimation of haemoglobin in blood, Define the Estimation of Hae...

Define the Estimation of Haemoglobin in Blood? Several methods are used to determine haemoglobin. In these methods hemoglobin is measured as oxyhaemoglobin, carboxyhaemoglobin,

Explain the process of determination of vitamin C, Determination of vitamin...

Determination of vitamin c Majority of chemical methods of determination is based on the rapid oxidation of ascorbic acid and therefore not too highly specific. Titration of

Prevention strategy for diverticular disease, Q. Prevention strategy for di...

Q. Prevention strategy for diverticular disease? The prevention strategy for the disease involves the following: • Eat a high-fiber diet (more than 15 g/day of crude fiber)

Sequence of cardiac activation, Sequence of Cardiac Activation Depolar...

Sequence of Cardiac Activation Depolarisation is initiated by an impulse form the SA node. Impulse spreads through both atria. Inter-atrial conduction and atrial myocardial

Cardiac resynchronization therapy, This innovative, pacemaker-based approac...

This innovative, pacemaker-based approach to the treatment of patients with heart failure who have a wide QRS complex (>140 ms) on 12-lead ECG aims at providing electromechanical c

Define historical example of virulence, Define Historical example of virule...

Define Historical example of virulence? The "classical dogma" of epidemiologists was that pathogens all the time evolve in the direction of lower virulence, eventually becoming

The best describes the acid properties of guanine, Which statement best des...

Which statement best describes the acid/base properties of guanine? -at pH, guanine will be negatively charge -at pH, guanine will be neutral -it will be an effective buff

How to defibrillate, How to Defibrillate? Three shocks should be given...

How to Defibrillate? Three shocks should be given initially in a 200J - 200J - 360J sequence. For most recoverable situations, 200J would be adequate and results in least myco

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd