Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Can our experiences change our brain, Can our experiences change our brain ...

Can our experiences change our brain A. It is now know by Scientists that brain is remarkably "plastic:" it continues to change during the course of life in accordance with our

Define general characters and classification of arthropoda, Define General ...

Define General Characters and Classification of Arthropoda? These are metamerically segmented animals with an exoskeleton of cuticle. Scliizocoelic coelom is much reduced and is

Explain phylum mollusca, Phylum Mollusca (47,000 species) The segmentat...

Phylum Mollusca (47,000 species) The segmentation and coelom may not exist. Diversification through modifications of head, foot; mantle and 'radula for feeding. .Visceralorgans

What are the problems regarding terrestrial environment, Q. What are the pr...

Q. What are the problems that vertebrates needed to solve to adapt to the terrestrial environment since they came from the aquatic habitat? How evolution does solve those problems?

Methods of prevention of flap tearing, Prevention of flap tearing Teari...

Prevention of flap tearing Tearing of a flap is a common complication of the inexperienced surgeon who attempts to perform a procedure using a flap that provides insufficient a

What is the life cycle of ascaris, Q. What is the life cycle of ascaris? ...

Q. What is the life cycle of ascaris? The Adult ascaris that live within the human intestine can release up to 200 thousand eggs a day. The eggs are eradicating with human fece

Explain sterilization, Explain Sterilization The physical process of ...

Explain Sterilization The physical process of killing microorganisms, including pathogenic and the bacteria,  vegetative forms and other spores on or in an inanimate object o

Which kind of gamete produced by a heterozygous individual, Which is the ki...

Which is the kind of gamete produced by a heterozygous individual? And what is the genotypical proportion of these gametes? The Heterozygous individuals, for instance, AA, prod

What are changes in st-segment, Q. What are changes in ST-Segment? Nor...

Q. What are changes in ST-Segment? Normal Exercise Electrocardiogram ST and J Point As the heart rate increases with exercise, a number of predictable changes occur in a

How you can repair ventricular aneurysm with a patch?, How you can Repair V...

How you can Repair Ventricular Aneurysm with a patch? Repair of Ventricular Aneurysm with a patch :  Originally described by Cooley, as endoaneurysmorra

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd