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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Define the importance of Iodine in human? Iodine derives the nutritional importance as a constituent of thyroid hormones, 3,5,3',5' tetraiodo-thyronine (thyroxine or T 4 ) and
Explain the Reinforcement of Patch using Fibrin Glue? Reinforcement of Patch using Fibrin Glue : In this technique Dacron graft is first sutured to the septum beyond i
Define Role in Glucose, Lipid and Nucleic Acid Metabolism? Chromium leads to a decrease in blood glucose concentration in people with elevated glucose levels and an increase in
How do i use the factor-label method to calculate the number of grams of KMnO4 that you would need to make 100 ml of a 0.0045 M potassium permanganate solution? Be sure to show all
How are radioactive probes useful in DNA fingerprinting? Radioactive probes bind to specific fragments in a DNA sample like a donor gene. A probe can identify which bacteria ha
Levels of organization of Matter- Metazoa The smallest structural units of all matter are subatomic particles, mainly electrons, protons and neutrons. The next larger units a
DNA sequencing is the process of reading the nucleotide bases in a DNA molecule. It includes any technology or method which is used to verify the order of the four bases- guanine,
Explain about the duration of exercise for diabetics patient After selecting an exercise, it is very important to know the vigorous nature, duration and timings of the exercise
Implant success 1) An individual, unattached implant is immobile when tested clinically. 2) A radiograph that does not demonstrate any evidence of perimplant radiolucency.
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