Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Skeleton, why do we get severe leg pains when the internal bone is cracked?...

why do we get severe leg pains when the internal bone is cracked?

Mature embryo, Mature Embryo A typical dicotyledonous embryo, as seen ...

Mature Embryo A typical dicotyledonous embryo, as seen in a median longitudinal section, consists of an embryonal axis having two broad cotyledons. The portion of embryonal ax

Types of endoplasmic reticulum, TYPES E.R. consists of two types -     ...

TYPES E.R. consists of two types -        1. Rough E.R.                2. Smooth E.R.   R o ugh E.R.   S m o o th E.R.

Explain nutritional effects of hemicelluloses, Nutritional effects Of Hemic...

Nutritional effects Of Hemicelluloses Hemicelluloses are classified under the category of soluble dietary fibers.  Hemicellulose is an indigestible complex carbohydrate that ab

What are some things that have algae in them, What are some things that hav...

What are some things that have algae in them? Yeast is considered Algae.

Pulse oximetry readings, Outline three client considerations when taking an...

Outline three client considerations when taking an accurate pulse oximetry reading.

Which term describes study of animal behaviour, Which of the below terms de...

Which of the below terms describes study of animal behavior based on comparisons, observation of patterns, and consideration of environmental adaptations? Is it: a) Ethology (p

Direct approach to counseling, Q. Direct Approach to counseling? The di...

Q. Direct Approach to counseling? The direct approach to counselling is also called as the problem-solving approach. The counsellor collects relevant information which aims to

Explain the interaction of vitamin a with iron, Explain the interaction of ...

Explain the interaction of vitamin A with Iron? Iron: Iron status of an individual correlates with vitamin A. The deficiency of  vitamin A has been found to be associated with

Define the chemical equation of the formation of bicarbonate, Q. What is th...

Q. What is the chemical equation of the formation of bicarbonate from water and carbon dioxide? What is the enzyme that catalyzes this reaction? The chemical equation of the ch

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd