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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Define Ion exchange chromatography? In this method a reversible exchange of ions is effected. Usually resins and ionic solutes are the two components involved in ion-exchange.
Imaginal Discs In the holometabolous larva, there are two cell populations: (1) The larval cells that are used for the larval structures and (2) The imaginal disc and th
Q. Which are the major positive ions found in living beings? The major cations found in living beings are the calcium cation (Ca++), the potassium cation (K+), the sodium catio
For homework, I had to prove that a certain catalyst was a protein, then tell how I would purify the protein, then tell how I would clone a human chromosomal region that contains t
Loading and Unloading of Sieve Tubes In order to understand the loading of food from manufacturing leaf cells to sieve tubes we must examine the anatomy of a minor vein shown
Photosynthesis is the most significant producer of molecular oxygen (O2) on our planet. From which molecule do oxygen atoms liberated by photosynthesis come? From which other molec
Water Reuse And Recycling Are Becoming Much More Common As Demands For Water Exceed Supply. Unplanned reuse occurs as the result of waste effluents entering receiving waters or g
Some of the situations that concern ethical decisions in nutrition care are as follows: Difficulty of adequate nutritional support of malnourished patients, a) A problem
Green leaves give off oxygen in sunlight Place some water weed under a funnel in a beaker of water reverse a test tube full of water over the tube of the funnel. Leave the appa
Q. Do the arteries that carry blood from the heart to the lungs contain arterial or venous blood? What happens to the blood when it passes through the lungs? Arteries of the pu
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