Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Resource use of biodiversity, Biodiversity is multiple resource based offer...

Biodiversity is multiple resource based offering us a range of products, materials and services. Our survival and well being of man-kind is directly dependent on biodiversity. Biod

Explain ventricular aneurysm in miscellaneous conditions, Indications for S...

Indications for Surgery :  95 per cent of ventricular aneurysms occur after transmural myocardial infarction. Trauma, Chaga's disease, sarcoidosis and congenital causes are the ot

What is catabolism, Q. what is Catabolism? Catabolism is a process of b...

Q. what is Catabolism? Catabolism is a process of breaking down large or complex molecules of food components into smaller components. These supply energy to the cell in a form

Physical and physiological changes - geriatric nutrition, Define Physical a...

Define Physical and Physiological Changes? Every stage has its unique requirements due to different changing needs. With respect to nutrition and health, four different basic a

Whcih group of individual animals and plants called species, Q. Whcih group...

Q. Whcih group of individual animals and plants called Species? A group of individual plants and animals that are fundamentally alike is treated as a ,species. Species is a par

How do malign neoplasias appear, Q. How do malign neoplasias appear? Th...

Q. How do malign neoplasias appear? The Neoplasias appear due to DNA mutations in genes that regulate the cellular proliferation thus making the cell lose its capacity to contr

How are nematodes characterized, Q. Nematode identity card. How are nematod...

Q. Nematode identity card. How are nematodes characterized according to examples of representing beings, type of symmetry basic morphology, , germ layers and coelom, digestive syst

Define colonization of the gut - probiotic effect, Define colonization of t...

Define colonization of the gut - probiotic effect? It is not clear how probiotics influence the flora and produce a beneficial effect. However, colonization of the gut appears

Applications in horticulture and agriculture, Applications in Horticulture ...

Applications in Horticulture and Agriculture While going round in public parks, you might have observed plants trimmed in such a way giving appearances of animals like camel,

What is standard operating procedure manual (sop), Question 1 What is s...

Question 1 What is standard operating procedure manual (SOP)? Do you think it is important to maintain SOPs in all the laboratories? List the advantages of maintaining SOPs in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd