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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
What is polyembryony? Polyembryony is the phenomenon in which a single embryo in its initial embryonic stage separates itself forming many new individuals of the similar sex an
Define Excretion of Methyl malonic acid in urine (Methyl malonil aciduria)? Methyl malonic acid is found only in traces in normal urine (1-2 mg/day). In vitamin B 12 deficie
about apiculture
Q. Explain Spoilage by fungi and bacteria? The dominant spoilage organisms in fruits are fungi, as their pH is low (normally 5),whereas the pH of vegetables is fiom 5.0 to 7
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Feeding Mechanisms of Animals All animals have evolved successful methods for extracting their required nutrition from the environment. Thus we find a diversity of feeding mec
Q. What are yeast? Yeasts are unicellular fungi, which are widely distributed in nature. They are somewhat larger than bacteria. The cell length is about 10μm and the diameter
Define Physical and Physiological Changes? Every stage has its unique requirements due to different changing needs. With respect to nutrition and health, four different basic a
Explain root pressure? How can you demonstrate it experimentally? What is the cytological basis of Down's syndrome? Give two physical symptoms of this disorder. Describe why the
Sulphur Sulphur is a component of the animo acids methionine, cysteine and cystine; the B-vitamins, thiamin and biotin; as well as a number of the organic compounds. Sulphate,
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