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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Explain the Colorimetric Method Glucose Estimation? Alkaline copper reduction methods are by far the most commonly used methods for glucose estimation. This time honoured metho
MUTATION THEORY (Hugo de Vries 1901) - New species are not formed by continuous variations but sudden appearance of variations i.e., mutation. Mutation are heritable and per
Q. How is the cerebrum anatomically divided? The cerebrum is divided into two cerebral hemispheres, the left and the right. Each hemisphere is made of four cerebral lobes: pari
Are proteins with the same number of each different amino acid that form them necessarily identical proteins? Even if many proteins have the similar number of each dissimilar a
Q. What is the neurotransmitter of the neuromuscular junction? How does the nervous system trigger muscle contraction? The nervous cells that trigger the muscle contraction are
Q. Counselling for day to day Management? As we know that diabetes is a chronic disease, a major part of its control requires counselling the patient and his family for day to
What is Modified Blalock-Taussig Shunt explain? This is usually done by interpositioning a PTFE (Goretex) graft of 3.5 or 4 mm in a neonate. It is better done by a left lateral
Food Intolerances Food Intolerances: The intolerances of the patient for specific food items must be assessed. For example, milk may lead to diarrhoea in some people whil
Tracheoles - Respiration The tracheal system consists of an air filled system of tubes - the tracheal tubes that branch repeatedly until they become fine as capillaries, and
Fumonisins The most recently characterized mycotoxins of any major significance in human health are the fumonisins produced by species of Fusarium, such as F. moniliforme. Like
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