Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Explain neurologic disease, Explain Neurologic disease For patients wit...

Explain Neurologic disease For patients with facial nerve palsy alone, oral doxycycline or amoxicillin may be effective. Patients with other neurologic involvement, such as men

What is quantitative techniques - microbial culture, What is Quantitative T...

What is Quantitative Techniques? Microbial quality of substances like food, milk, air, water, soil etc. can be assessed both qualitatively and quantitatively. Quantitative enum

How are chromosomes classified, How is the chromosome region where the cent...

How is the chromosome region where the centromere is located called? How are chromosomes classified in relation to the position of their centromere? The chromosome region where

Define absorption of zinc - micro minerals, Define Absorption of Zinc - Mic...

Define Absorption of Zinc - Micro Minerals? Like iron, zinc also needs to be liberated from food prior to absorption. During digestive process; proteases, nucleases and hydroch

Male accessory sex organs, Normal 0 false false false E...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What is the difference between rna and dna, Q. Concerning the nitrogen- con...

Q. Concerning the nitrogen- containing bases that participate in nucleotides, what is the difference between RNA and DNA? In DNA nucleotides can be formed of thymine (T), adeni

What is the concept of lophophore, What is the concept of Lophophore? A...

What is the concept of Lophophore? A unique double ring of hollow ciliated tentacles that surround the oral opening in some animal phyla. There is some debate as to whether the

Psychology, What name is given to the mental processing of sensory informat...

What name is given to the mental processing of sensory information?

What do you mean by sex ratio, What do you mean by sex ratio? Sex Rati...

What do you mean by sex ratio? Sex Ratio is defined as "the number of females per 1000 males". Sex ratio is an important social indicator to measure the extent of prevailing e

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd