Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Pathogenic bacteria, Pathogenic bacteria Heterotrophic bacteria could ...

Pathogenic bacteria Heterotrophic bacteria could be divided into saprophytes and parasites. Saprophytes normally do not cause any disease and they survive on dead or decaying

Can you explain process of ovulation, Q. What are the hormones that promote...

Q. What are the hormones that promote the release of the female gamete from the follicle and at which day of the menstrual cycle does this phenomenon happen? What is this event cal

Define aortic regurgitation, Q. Define Aortic regurgitation? Aortic reg...

Q. Define Aortic regurgitation? Aortic regurgitation is a common valvular disease that may present chronically or acutely. Normally the integrity of valve closure depends upon

Biogeochemical cycle, what are the effects of mining and farming on the pho...

what are the effects of mining and farming on the phosphorus cycle?

Body temperature and room temperature , You ran both a body temperature and...

You ran both a body temperature and room temperature test for each enzyme you tested. Did the two temperatures seem to have an effect on the enzyme Exercise? Why or why not?

Etiology, Etiology: This is the study of origin of diseases. In other words...

Etiology: This is the study of origin of diseases. In other words we can say etiology is the study of causation and origination. Etiology most commonly used in philosophical theori

Cytoplasmic bridge formed during the conjugation of paramoec, Cytoplasmic b...

Cytoplasmic bridge formed during the conjugation of paramoec: The two paramoecia that undergo conjugation are called conjugants. During conjugation, the two conjugants com

Explain cardiac examination auscultation and its techniques, Explain cardia...

Explain cardiac examination auscultation and its techniques? Auscultation of the heart sound and associatcd sound is very important to diagnose the cardiovascular diseases. It

Define nitrogen estimation - kjeldahl''s method, Define Nitrogen Estimation...

Define Nitrogen Estimation - Kjeldahl's Method? Nitrogen in the protein is estimated by a method referred to as Kjeldahl's method. Although over a period of time many other met

Explain dietetics, Explain Dietetics Dietetics  has been defined as  t...

Explain Dietetics Dietetics  has been defined as  the science and art of feeding individuals based  on the principles of  nutrition. It can also be  said  to  be  the "science

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd