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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Which of the following properties of dsDNA is imprtant for DNA purification? A. Hydrophilic B. Positively charged phosphate backbone C. Can only bind to divalent cations
Fertilization - Pre-Embryonic Development Ovulation generally takes place between 9th to 14th day after the end of last menstrual period. Fertilization of oocyte initiates dev
Embrylogical Devlopment of Hypospadias The development of the penis and scrotum is intluenced by the testes. In the absence of the testes, the female structures of the
What is Classic Blalock-Taussig BT Shunt in Shunt Operations ? Subclavian artery, which arises from innominate artery, is anastomosed to pulmonary artery. In a patient with lef
Explain Horizontal Full Mucoperiosteal Flaps - Endodontic Surgery a) Also called: Envelope flap, b) Only a Horizontal incision, without the vertical releasing incision
Explain the introduction of history of mart disease? Cardio-vascular diseases (CVD) are the leading causes of death and disability both in the developed and the developing na
Glycosylation: The covalent addition of the sugar moities to N or O atoms present in the side chains of various amino acids of certain proteins, usually occuring within the Golgi
The cardiac output is the volume of blood pumped from the heart every minute. It is obtained by the volume pumped with each beat (stroke volume) multiplied by the heart rate. The c
What is an antigen? Antigen is any substance, particle or infectious agent known as foreign to the body. The contact of the antigen with the body promotes a defense reaction ag
human digestive system and the functions
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