Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Human ovum, A pair of ovaries are the reproductive organs in woman. They...

A pair of ovaries are the reproductive organs in woman. They are present just below the kidneys in the abdominal cavity. Each ovary has several sac like structures called ova

Show examples of cnidarians, Q. What are few examples of cnidarians? In whi...

Q. What are few examples of cnidarians? In which environments can these animals be found? Hydra, Jellyfish, sea anemones and corals are good examples. All of them are aquatic,

Ideal characteristics of oxygenator, Ideal Characteristics 1) Maximize...

Ideal Characteristics 1) Maximize gas transfer (oxygen, carbon dioxide and anaesthetic gases) 2) Minimize blood trauma 3) Good heat transfer efficiency 4) Minimize

Cellular and molecular events of bone formation, Q. Summarize the cellular ...

Q. Summarize the cellular and molecular events of bone formation? The events involved in osseous wound healing at implants recapitulate the events of wound healing. Haemostasi

Explain metastatic carcinoma, Explain Metastatic carcinoma Metastatic ...

Explain Metastatic carcinoma Metastatic carcinoma:- Cancer that can be  transferred from one part of the body to other unrelated parts.

What is biological contaminants, Q. What is Biological Contaminants? Yo...

Q. What is Biological Contaminants? You may recall reading about food borne diseases caused by the consumption of contaminated food items in the last unit. In the

Transported soils-types of soils, Transported Soils These soils are for...

Transported Soils These soils are formed from the weathered material which is transported and deposited away from the site of origin. Depending upon the nature of the transport

Organization levels of life, Q. Define the Organization Levels of Life? ...

Q. Define the Organization Levels of Life? Ans. The cell is the basic unit of life - the smallest unit that can carry on all the processes of life. Organisms that consist of a

Treatment and management of hypertension, Q. Treatment and Management of Hy...

Q. Treatment and Management of Hypertension? The first choice of treatment and management of primary hypertension is through behaviour modifications pertaining to food choices

Explain microbiology of vegetables, Q. Explain Microbiology of vegetables? ...

Q. Explain Microbiology of vegetables? Ans. All of us are well aware that vegetables are grown in soil, which, we already know, are a rnajor support system for the mi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd