Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Explain about the epilepsy, Explain about the Epilepsy? Epilepsy is a n...

Explain about the Epilepsy? Epilepsy is a neuromuscular disorder in which transient seizures recur due to an abnormal brain activity. The brain, through all orderly electric ex

Male gamete and the female gamete in a flowering plant, What is (a) the mal...

What is (a) the male gamete, and (b) the female gamete in a flowering plant?   (a) The male gamete in a flowering plant is the pollen grain (strictly, the gamete is the male

Nutrition - heterotrophic nutrition, HETEROTROPHI C NUTRITION Animals ...

HETEROTROPHI C NUTRITION Animals derive organic food materials by consuming products of plants - Heterotrophic nutrition. T ype s of Heterotrophic Nutrition (i ) H

Explain the chloride permeability, An impermeable membrane separates one li...

An impermeable membrane separates one liter of a 1M KCl solution in the left compartment from one liter of 1M NaCl solution in the right compartment.  At 2 AM today the membrane be

Future challenges for scaling from individual to ecosystems, Define Future ...

Define Future challenges for scaling from individual to ecosystems? Scaling of biogeochemical fluxes in terrestrial systems has confirmed much harder. While ecosystem ecologist

Wbc.., Ask question #Minimuwwm 100 words accepted#

Ask question #Minimuwwm 100 words accepted#

Illustrate dilated cardiomyopathy, Q. Illustrate Dilated cardiomyopathy? ...

Q. Illustrate Dilated cardiomyopathy? It is a disease of unknown etiology, affecting myocardium. Its diagnosis is established by presence of left ventricular dilatation and sys

Why conserve wild life ?, WHY CONSERVE WILD LIFE ? 1 .       ECONOMIC...

WHY CONSERVE WILD LIFE ? 1 .       ECONOMIC IMPORTANCE OF WILD LIFE - (i) Plants - (ii) Animals - (iii) Micro organisms - 2 .       ECOLOGICAL BALANCE -

Uses of plural embryos, Uses of Plural Embryos Although the basic tha...

Uses of Plural Embryos Although the basic that trigger causes polyembryony are not fully understood, there has been no dearth of interest in exploiting supernumerary embryos.

Was there molecular oxygen in the earths primitive atmospher, Was there mol...

Was there molecular oxygen in the earth's primitive atmosphere? How has that molecule become abundant? The presence of the molecular oxygen in the primitive atmosphere was prob

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd