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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
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What is the difference between the alpha helix and the beta-sheet protein conformations? Ans) Alpha helix and beta-sheet conformations are the two major types of secondary struc
Spermiogenesis - Spermatogenesis At the end of the meiosis the spermatids appear as simple spherical cells with a centrally located nucleus. Their differentiation into sperm r
Point mutations are often caused by substances called mutagens explain? Point mutations are often caused by substances called mutagens. Base analogs, such as 5-bromouracil, a t
Individual Psychotherapy: It is conducted on one to one basis. It is effective when a therapeutic rapport develops between patient and therapist. The common types of indiv
Explain the Aseptic Technique - Sub Culturing? Aseptic technique is a series of procedures used to prevent contamination of cultures and sterile culture media. It is one of the
In these disinfectants, iodine is incorporated along with a detergent. They are not as effective as hypochlorites against bacterial spore but also get inactivated in the pres
What are antioxidants? To put it simply, antioxidants are important naturally occurring nutrients, (vitamins, minerals) which help to protect body from certain types of cancers
What is the rule for the pairing of nitrogen-containing bases in the DNA molecule? And in the RNA? The rule for the pairing of nitrogen-containing bases of the polynucleotide c
sample assignment on tagmatizatio
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