Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Role of private sector in health care, Role of Private Sector in Health Car...

Role of Private Sector in Health Care One of the biggest policy challenges for the government is to decide on the appropriate areas and a suitable policy framework for having

Long lasting vaccine against common cold, Q. Why does not a long lasting va...

Q. Why does not a long lasting vaccine against common cold exist yet? Viruses that present a high mutation rate like the virus that causes the common cold escape easily from th

What is forebrain, Q. What is Forebrain? Forebrain: Found in the area o...

Q. What is Forebrain? Forebrain: Found in the area of the forehead, this part of the brain is concerned with all the emotions, planning, organising, reasoning, memory, movement

Conditions necessary for outbreak of botulism, Q. Conditions Necessary for ...

Q. Conditions Necessary for outbreak of botulism? The following conditions are necessary for an outbreak of botulism: 1. Presence of spores of C. botulinum of type A, B o

What is a monohybrid cross, A monohybrid cross: A.Determines the genetic ma...

A monohybrid cross: A.Determines the genetic makeup of an organism B.always involves homozygous alleles. C.always involves organisms that are heterozygous at all loci. D.Always inv

Dopamine for working memory, Dopamine is important for working memory and d...

Dopamine is important for working memory and drug that increases the level of dopamine in the brain or facilities the action of dopamine, enhances working memory capabilities.Dopam

Ontogeny, Ontogeny: This is the study of history of an individual. Ontogen...

Ontogeny: This is the study of history of an individual. Ontogeny is also known as ontogenesis and morphogenesis. Ontogeny describes the origin or the development of an organism -

Lipids - digestion process, Lipids are essential fats that have much import...

Lipids are essential fats that have much importance to the human body. Lipids are biological molecules that are insoluble in aqueous solutions and soluble in organic solvents are c

Bats , 1. Explain how bats determine the distance (range) of their target u...

1. Explain how bats determine the distance (range) of their target using echolocation. Be sure to describe the properties of their call that make it possible (use a sketch), and ho

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd