Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Explain uses of modified starches in food, Uses of modified starches in foo...

Uses of modified starches in food and confectionery industries Visit a grocery shop and look out for products such as the instant desserts, jelly beans, salad dressings, tomato

What are the female pronucleus, What are the female pronucleus and the male...

What are the female pronucleus and the male pronucleus? The female pronucleus is the proper haploid nucleus of the egg cell. Male pronucleus is the haploid nucleus of the sperm

Explain the objective of cardiac rehabilitation, Explain the OBJECTIVE of C...

Explain the OBJECTIVE of Cardiac Rehabilitation ? After going through this unit, you should be able to: 1 identifies the eligible candidate for cardiac rehabilitation and be

Differences between prokaryotes and eukaryotes, Differences between Prokary...

Differences between Prokaryotes and Eukaryotes It is obvious that there are a number of differences between them. Prokaryotes, like bacterial cells are generally much smaller,

What is shunts in congenital heart surgery, What is shunts in Congenital He...

What is shunts in Congenital Heart Surgery? Shunts : Blalock Shunts are connections between systemic arteries such as a subclavian arteiy with its respective pulmonary arteiy

How do arthropods grow, How do arthropods grow? Because of the presence...

How do arthropods grow? Because of the presence of exoskeleton the growth of an arthropod is periodical. During the growth period the animal loses the exoskeleton, grows and de

What is the rh typing of the mother, What is the Rh typing of the mother an...

What is the Rh typing of the mother and of the fetus in the hemolytic disease of the newborn? In the hemolytic disease of the newborn the mother is Rh- and the fetus Rh+. In th

Biogeochemical cycling - nutrient cycles, Biogeochemical Cycling - Nutrient...

Biogeochemical Cycling - Nutrient Cycles The energy flows through ecosystems enabling the organisms to perform various kinds of work, and is ultimately lost as heat. It is gon

List the benefits of exercises, List the benefits of exercises. Benefit...

List the benefits of exercises. Benefits of exercise are : - improves sugar control - decreases HbA1c level - decreases  blood pressure - decreases triglyceride lev

Objectives of counselling technique of diabetic patient, - Highlight the is...

- Highlight the issues related to diabetes mellitus. - Apply different types of counselling skills to counsel patient with Diabetes and their family members - Develop pre-req

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd