Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Natural selection of heterogeneous environments, While describing normalisi...

While describing normalising selection it was said that in uniform environments selection limits the variability of populations and evolves a genetic homeostasis. The type of selec

Fluorescence microscopy, Fluorescence Microscopy Certain compounds when e...

Fluorescence Microscopy Certain compounds when exposed to short wavelength radiations, e.g., UV and X-rays. absorb and emit energy as light of a longer wavelength. This process o

Temperate shrublands, These are areas where woody shrubs predominate rather...

These are areas where woody shrubs predominate rather then trees. In regions with a Mediterranean type of climate i .e., hot dry summers and cool wet winters, shrubs grow close tog

Isolation of dna fragments, Isolation of dna fragments bound strongly with ...

Isolation of dna fragments bound strongly with protein 1.     Through pcr Pcr permits isolation of dna fragments from genomic dna through selective amplification of a spe

Into which classes are mollusc divided, Into which classes are mollusc divi...

Into which classes are mollusc divided? What are some representing beings of each class? The phylum Mollusca is separated into five major classes: pelecypods, or bivalves (Pele

Inrinsic conduction system of the heart, The cardiovascular system controls...

The cardiovascular system controls the movement of blood through thousands of miles of capillaries so that every tissue  in every part of the body is perfused. Essential nutrients

Explain dextrins, Dextrins Dextrins are also polymers of D-glucose held...

Dextrins Dextrins are also polymers of D-glucose held by a(l,4) glycosidic linkages. Dextrins are,  in  fact,  formed due to partial hydrolysis of starch by  enzymes such as sa

What is biological magnification, a) What is biological magnification? ...

a) What is biological magnification? b) Describe how DDT as a water pollutant undergoes biological magnification?

What is demographic transition, What is Demographic Transition? The who...

What is Demographic Transition? The whole process of change with regard to population size and characteristics is called demographic transition. In order to understand this ter

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd