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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Ethidium Bromide intercalates within structure of the nucleic acids in such a manner that they fluoresce under the UV light. Ethidium bromide staining is generally used to visuali
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Consider a simple spherical model cell that consists of cytoplasm and a plasma membrane. The cell's initial volume is 2 nL and contains 0.2 M protein. The cell is placed in a la
What is vision? Why is vision important for life on earth? Vision is the ability of some living beings to perceive, to differentiate and to interpret luminous stimuli. Visio
In many countries of Asia biomass contributes l argely as a source of primary energy. People in rural areas at the peripheries of forests, continue to collect fuel wood from adjace
Q. What is Glycosylated Haemoglsbin? Glycosylated haemoglobin values give important diagnostic inferences regarding the recent past of a diabetic i.e. how well he/she managed t
The human skeleton has evolved from that of four-legged animals. Unfortunately, the adaptation is far from perfect; thus, our upright posture causes problems like backage and the k
Locomotion in Echinodermata Locomotion in echinoderms is accomplished through a unique canalicular system which is termed as the water-vascular system. This system is charact
Skeletons of radiolarians The skeletons of radiolarians and foraminiferans form a primary constituent of ocean bottoms where they form 30% or more of the sediment. It is calle
Meteorological Factors and Air Pollution Air pollution levels in a region are affected by wind, location, topography, precipitation and temperature inversions. Wind can carry
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