Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Explain major role in contributing towards motivation, Major role in contri...

Major role in contributing towards motivation. Psychological factors: Depression, anxiety or phobia induced by illness,  life- style changes or medication effects, may hinder

How many heterozygote ''carriers'' for this disease, Phenylketonuria is a s...

Phenylketonuria is a severe form of mental retardation caused by a recessive allele. The condition affects about 1 in 25,000 newborn babies. In a population of 250,000 people, how

Explain carrageenan, Carrageenan Carrageenan is a collective term for p...

Carrageenan Carrageenan is a collective term for polysaccharides prepared by alkaline extraction (and modification) from red seaweed (Rhodophycae). Carrageenan is a sulfated ga

Neck examination of new born, Neck Inspect and palpate the neck for an...

Neck Inspect and palpate the neck for any mass, check if neck is short, and webbed which is indicative of Turner's syndrome. Palpate posteriorly along cervical spine; later

Animal nutrition - zoology, Anima l Nutrition Nutrition is the procu...

Anima l Nutrition Nutrition is the procurement of substances necessary for growth, maintenance and activities of a living body. The substances used for nutrition are called

State the important role of microorganisms, State the important role of mic...

State the important role of microorganisms The important role of microorganisms was recognised as early as 1838, when J.B. Boussingeutt a French agricultural chemist observed t

Cytosol of prokaryotes, The  citric  acid  cycle  functions  in  the  mitoc...

The  citric  acid  cycle  functions  in  the  mitochondria   of  eukaryotes  and  in  the cytosol of prokaryotes. The Succinate dehydrogenase, the only membrane-bound enzyme   in t

Life cycle of filarial worm, Life cycle of Filarial worm Filariasis : ...

Life cycle of Filarial worm Filariasis : It is popularly known as elephantiasis. It is caused by a nematode parasite, wuchereria bancrofti, and W.Malayi. Parasite : Wucher

Explain about the lignans, Explain about the Lignans? Lignans are diphe...

Explain about the Lignans? Lignans are diphenolic compounds formed by dilnerization of 2 cinnamic acid residues. Most lignans apparently pass through the GIT as fibre. Some lig

What is autophagic intracellular digestion, What is autophagic intracellula...

What is autophagic intracellular digestion? Why is this type of intracellular digestion intensified in an organism undergoing starvation? Autophagic intracellular digestion is

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd