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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Explain the Secondary structure of proteins The secondary structure of a protein involves the way that the chain of amino acid either twists or folds back upon itself to form a
Define Reagents for Measurement of pH? Buffer solutions of pH 4, pH 7 and pH 9.18 for calibration and an unknown solution (i.e. solution of unknown pH) You can prepare these
Explain Resistance to Infection in Nutritional Care? Amino acids help to build the body's defence mechanisms like antibodies, blood cells, hormones and enzymes so as to prevent
Looking at what the academic world is publishing, microarray research is actually hot right now, and people haven't quite figured out what the best way is (some might argue if we s
a) In humans, is it the sperm or the ovum which verifies the sex of the offspring? b) Give a reason for your answer. (a) The sperm verifies the sex of the off
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Explain Malnutrition Malnutrition, impaired immunity and infection can form a triad or vicious cycle, as illustrated in Figure, which works synergistically and thus worsens the
Sunlight - Aquatic Habitats Sunlight is a major limiting factor for water bodies, since light rapidly diminishes as it passes down the column of water. The upper layers of the
components of human skeleton
DISORDER S OF FEMALE REPRODUCTIVE SYSTEM - (i) Sterility (ii ) Menstrual irregularity - This may be amenorrhea (absence of M.C.) or excessive bleeding (
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