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Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.
Please consider the phenomenon of bacterial resistance to antibiotics. How did the combination of human advances and basic evolutionary principles lead to this situation? How is it
What are the positions of actin and myosin molecules in the sarcomere before and during the muscle contraction? Schematically actin filaments attached perpendicularly to both s
State the symptoms of epilepsy Three particular symptoms are found in many types of epilepsy: 1) An aura, or warning, of impending seizure: This aura may take the form of a
Caseous lymphadenitis Caseous lymphadenitis is a chronic disease of sheep and goats. It is characterized by abscess formation in lymph nodes and sometimes in lungs and other organ
A plant grown from one of Mendel's yellow peas is selfed. Five progeny peas are obtained from this self and they are all yellow. If the original selfed plant had been homozygous, w
a) Name the mineral elements needed by (i) bones, (ii) red blood cells, (iii) the thyroid gland b) Which of these elements is (i) present in milk, (b) lacking in milk?
Q. Causes of gastro oesophageal reflux disease? GERD may develop due to any of the following reasons: • decreased muscle tone or abnormal relaxation of the LES, • reduced st
Define Impediments to infuse biology - Lack of trained people? At present, biologists as a rule have not been trained sufficiently in quantitative approaches. A heavy dose of m
How is the circulatory system of reptiles characterized? What is the basic difference among the reptile and the amphibian heart? The circulatory system of beings of the class R
For a sample containing both protein and nucleic acid, the absorbance measured at a particular wavelength will be the sum of contributions from both types of molecule. Using the pr
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