Gateway reaction-entry clone and destination vector, Biology

Assignment Help:

1530_Gateway Recombination reaction.png

(1) From the above gateway reaction (entry clone and destination vector);

(i) which plasmid will be selected for when transformed into E.coli(A or B) and why?

(ii) which antibiotic would you included in the selection?

(iii) which strain of E. coli would you transform the gateway reaction into?

(iv) which strain of E. coli do you need to maintain and amplify the entry clone

(v) which strain of E. coli do you need to maintain and amplify the destination vector.


Related Discussions:- Gateway reaction-entry clone and destination vector

Endocytosis and exocytosis, Endocytosis and Exocytosis Endocytosis is the...

Endocytosis and Exocytosis Endocytosis is the process by which substances are brought into the cell from outside by formation of membrane bound vesicles. These extra cellular sub

Evaluate the magnitude of st depression, Q. Evaluate the Magnitude of ST De...

Q. Evaluate the Magnitude of ST Depression? It is intuitive that the magnitude of ST depression should correlate with the degree of the ischaemia. In patients with left main or

Sources of air pollution, Combustion of fossil fuels and wood in industries...

Combustion of fossil fuels and wood in industries, automobiles, aircrafts, railways, thermal plant kitchens etc., agricultural operations and industrial processing are major source

What are the main prophylactic measures against malaria, Q. What are the ma...

Q. What are the main prophylactic measures against malaria? The major preventive measures against malaria are the elimination of the treatment of infected people, vector mosqui

What percentage of acid is not ionized, A weak acid (HA) has a pKa of 4.691...

A weak acid (HA) has a pKa of 4.691. If a solution of this acid has a pH of 4.920, what percentage of the acid is not ionized? (Assume all H in solution came from the ionization of

Biological oxygen demand - effect on water bodies, Biological Oxygen Demand...

Biological Oxygen Demand - Effect on Water Bodies Addition of biodegradable matter exerts demand on oxygen of the water body. The demand of oxygen is directly related to incre

Nursing care and treatment for cleft palate, Nursing Care and Treatment  ...

Nursing Care and Treatment   The only treatment is surgical repair and child may be operated before 18 months as per the choice of surgeon so  that speech pattern of child may

Are the extraembryonic membranes the same in all vertebrates, Q. Are the ex...

Q. Are the extraembryonic membranes the same in all vertebrates? The presence of each extraembryonic membrane differs according to the vertebrate class. In amphibians and fi

Skeletal muscle need an antagonistic partner, Why does each skeletal muscle...

Why does each skeletal muscle need an antagonistic partner? Because muscles can only contract and relax, each skeletal muscle requires an antagonistic partner to extend it afte

Population genetics and evolution, POPUL A TIO N GENETICS AND EVOLUTION-...

POPUL A TIO N GENETICS AND EVOLUTION- Population is the number of all individual of same species in a defined area at a particular time. As per modem synthetic theory pop

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd