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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
Acellular and Cellular Organisms you should be able to characterise living and non- living matter. However, the boundary-line between the two is not very precise. As you have
In phylum colenterata if tissues are present in the body of an organism then why organs are not present as group of tissues makes an organuestion..
Explain Cultivation of microorganisms It is for the production of food colourants has attractions, these must be measured against the financial legislative and user constraint
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In 1809, Maggiolo described a process of fabricating and inserting gold roots into freshly extracted sockets. The implant was constructed from the three pieces of gold which were s
Define the Disc Electrophoresis? Disc electrophoresis has improved resolution and strong bands are obtained. It requires a two-gel system and several different buffers. The run
Q. Investigations process of acute pericarditis? 1) Blood Examination Erythrocyte sedementaion rate (ESR) may be elevated in tuberculous; collagen and purulent pericarditis.
Cytoplasmic inclusions are cytoplasmic molecular aggregates, like as pigments, organic polymers and crystals. They are not considered cell organelles. Fat drops and glycogen gra
Q. Why is waste considered one of the major environmental issues? The environmental problem regarding waste worsens with industrial development and the global growth of consump
I am trying to get help on the creation of a dataset for protein prediction in matlab
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