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Freeze Fracture Electron MicroscopyFreeze fracture electron microscopy i a technique lo reveal the surface features of hydrophobic interior of cell membrane. In this technique, the cells/tissues are first frozen in liquid freon at - 179°C. The frozen block is cut with a cold knife blade alongwith the planes of weakness, run through each cell. Generally, these planes occur between the two layers of lipid molecules of the membrane. The plane of fracture at these places exposes the interior of a plasma membrane (Figure shown below).
The newly exposed faces of a plasma membrane, the exoplasrnic fracture face (EF) and the protoplasmic fracture face (PF) are covered with a very thin layer of platinum by dissolving away the organic material. The freshly fractured membrane may be covered with platinum and carbon to dtain replicas (exact copies) of the surface and this is observed under a TEM. This technique demonstrates that intercellular junctions (where cells make contact with each other) are composed of specilaised membrane protein.
what term is used to indicate its grooves?
Explain the term - Neurochemical Manipulations Neurochemical and immunological methods have been used to identify groups of neurons in the central nervous system that use speci
type study of polystomella
Equipment : Basically, it has an intra aortic balloon pump. A balloon with a capacity of 40 ml is passed percutaneously through the femoral artery up to the upper end of descen
peptide bond
Characteristics of Ammonium Transport System The following are the characteristics of ammonium transport system: Ammonium transport system is ammonia' repressible
Cardiac Care on Admission (First two hours) ECG is monitored by more than one lead (three to five). Left atrial pressure, arterial BP, central venous pressure, respiration
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Or nit h o s i s (psittacosis) This is an important zoonotic bacterial infection and causes disease in humans and birds. Collectively, these conditions are called as chla
Q. How are the female gametes of gymnosperms formed? What is the relationship between this process and the concept of alternation of generations? In the female strobiles (cones
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