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Fluorescence MicroscopyCertain compounds when exposed to short wavelength radiations, e.g., UV and X-rays. absorb and emit energy as light of a longer wavelength. This process of emitting light from a compound is known as fluorescence and is now commonly used in microscopy. Compounds which naturally emit light exist in most living tissues. For example, the mitochondria of liver and kidney, collagen and chlorophyll give a strong fluorescence. Such a type of natural fluorescence is called as autofluorescence. Sometimes fluorescent dyes llKe rhodamine, quinacrine etc. called fluorochromes are used to stain cell components to produce secondary fluorescence. Such important dyes are also used in the study of chromosome behaviour.The fluorescent microscope is essentially an ordinary optical instrument. It has been modified by the incorporation of special filters. These filters allow only the required wavelength of light to pass and cause fluorescence in the specimen. The most significant application of fluorescent microscopy is in the field of immuno-fluorescent,antibody labelling technique about which you will study later in this Unit.
Triacylglycerols are synthesized from fatty acyl CoAs and glycerol 3-phosphate.The glycolytic intermediate dihydroxyacetone phosphate is first reduces to glycerol 3-p
ORGNELS WITH SINGLE WALL
Define Glycogenolysis Glycogenolysis : degradation of glycogen.
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Rash Diseases such as Lyme disease and SLE which present with rash may be mistaken for ARF. Lyme disease presents with characteristic rash and arthritis (which appears 1 to 2
It is a complex of antibody bound to antigen, which contains complement components.
Question 1: Describe the term Intellectual Property Rights. Show the difference between Patents, industrial designs, and trademarks. Define the term Intellectual Property
Recently, the media have promoted the idea of consuming only locally grown, organic produce. Are there any nutritional values that might be present in locally grown produce that mi
Define Causes of iron Deficiency Anaemia? Anaemia is a condition in which the blood cannot carry enough oxygen. This may be because there are fewer red blood cells than normal,
SIGNIFICANC E OF GASTRULATION - It results in the formation of three germ layers. It also resutls in the formation of different organs & organ systems. Gastrulation is e
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