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Fluorescence MicroscopyCertain compounds when exposed to short wavelength radiations, e.g., UV and X-rays. absorb and emit energy as light of a longer wavelength. This process of emitting light from a compound is known as fluorescence and is now commonly used in microscopy. Compounds which naturally emit light exist in most living tissues. For example, the mitochondria of liver and kidney, collagen and chlorophyll give a strong fluorescence. Such a type of natural fluorescence is called as autofluorescence. Sometimes fluorescent dyes llKe rhodamine, quinacrine etc. called fluorochromes are used to stain cell components to produce secondary fluorescence. Such important dyes are also used in the study of chromosome behaviour.The fluorescent microscope is essentially an ordinary optical instrument. It has been modified by the incorporation of special filters. These filters allow only the required wavelength of light to pass and cause fluorescence in the specimen. The most significant application of fluorescent microscopy is in the field of immuno-fluorescent,antibody labelling technique about which you will study later in this Unit.
Define about the Food Production? We know that agriculture comprises the major source of food production. This is very true in a country like ours where the majority of the pop
Carbohydrates are the next important nutrients as they have to perform a variety of functions such as: 1) Provide major source of energy 2) Breakdown of fatty acids and preve
if i start in the superior mesenteric vain where do i have to go through to get to the left anterior circumflex humeral artery. by the way of the hepatic portal vein and the heart
Explain about the Demand curve or schedule in short. The demand curve or demand schedule shows the total quantity purchased through all buyers, within aggregate, into the marke
What is the primary energy source for life on earth? The primary energy resource for life on earth is the sun. The sun plays the vital role of keeping the planet warmed and it
Q. Show the Pre-Diabetes Conditions? Pre-diabetes is a condition which may occur between normal and diabetes stages. It is important for you to know about this group of people,
Glycosylated Hemoglobin (HbA1c) HbA1c should be done routinely for diabetic patients. It reflects the average glycemic (sugar) control over the previous few months and should b
A protein that is normally a single-pass transmembrane protein is absent from the cell surface of the mutant cell line YTM-15. When labeled so that the protein can be localized, yo
Explain Potts Shunt in palliative operations ? This is a similar shunt done through a left thoracotomy. Anastomosis is done between left pulmonary artery aid descending thoraci
In which areas of the globe is gymnosperm abundance noteworthy? These plants are the typical vegetation of cold regions as the taiga, or boreal forest, of the northern hemisph
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