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Fluorescence MicroscopyCertain compounds when exposed to short wavelength radiations, e.g., UV and X-rays. absorb and emit energy as light of a longer wavelength. This process of emitting light from a compound is known as fluorescence and is now commonly used in microscopy. Compounds which naturally emit light exist in most living tissues. For example, the mitochondria of liver and kidney, collagen and chlorophyll give a strong fluorescence. Such a type of natural fluorescence is called as autofluorescence. Sometimes fluorescent dyes llKe rhodamine, quinacrine etc. called fluorochromes are used to stain cell components to produce secondary fluorescence. Such important dyes are also used in the study of chromosome behaviour.The fluorescent microscope is essentially an ordinary optical instrument. It has been modified by the incorporation of special filters. These filters allow only the required wavelength of light to pass and cause fluorescence in the specimen. The most significant application of fluorescent microscopy is in the field of immuno-fluorescent,antibody labelling technique about which you will study later in this Unit.
Petrol or gasoline can be synthesized by following three methods: 1. Polymerisation 2. Fischer - tropsch method 3. Bergius process polymerisation In
During mitosis ER and nucleolus begin to disappear at: 1. Late prophase 2. Early metaphase 3. Late metaphase 4. Early prophase Late prophase
Q. What are vitamins? What are the major vitamins needed by humans? Most vitamins are coenzymes fundamental substances for the enzyme functioning that are not produced by the o
Hydrogen ( h) has 1 electron; Carbon ( c) has 6 electrons, use the octet rule to determine which of the following is most stable. Note: some of these may have double bonds. A. O
Ecosystems main functions are physical, chemical and biological processes. Which is required for if maintenance of ecosystem. Some important function- 1. Production of bi
Q. How is the gastric mucosa protected from the acid pH of the stomach? The gastric epithelium is mucus secretory that is it produces mucus, the mucus covers the stomach wall p
How does a plasma membrane regulate movement of molecules into and out of a cell?
Define the Stress Response - nutrition during stress? The terms trauma, stress, shock are very often used interchangeably and encompass a variety of conditions such as sepsis
List and briefly describe the mechanisms that prevent acid-base imbalances from appearing in the body. In addition, indicate where each mechanism functions most significantly.
Percussive instruments -this method used a selected and controlled percussive removal force -Instruments delivers an impact either directly to a restorative or indirectly t
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