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Fluorescence MicroscopyCertain compounds when exposed to short wavelength radiations, e.g., UV and X-rays. absorb and emit energy as light of a longer wavelength. This process of emitting light from a compound is known as fluorescence and is now commonly used in microscopy. Compounds which naturally emit light exist in most living tissues. For example, the mitochondria of liver and kidney, collagen and chlorophyll give a strong fluorescence. Such a type of natural fluorescence is called as autofluorescence. Sometimes fluorescent dyes llKe rhodamine, quinacrine etc. called fluorochromes are used to stain cell components to produce secondary fluorescence. Such important dyes are also used in the study of chromosome behaviour.The fluorescent microscope is essentially an ordinary optical instrument. It has been modified by the incorporation of special filters. These filters allow only the required wavelength of light to pass and cause fluorescence in the specimen. The most significant application of fluorescent microscopy is in the field of immuno-fluorescent,antibody labelling technique about which you will study later in this Unit.
ATP-sensitive potassium channel A. The channel is a spanning protein with a receptor site for ATP located on an intracellular region of the protein. B. When blood plasma lev
Q. How is HIV transmitted? What is the disease caused by this virus? The HIV (human immunodeficiency virus) is supposed to be transmitted through blood, semen, vaginal secretio
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Explain Counter Stain - Ziehl-Neelsen Method? Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs c
An A=T mispairing leads to an A=C substitution. The other DNA helix will contain a(n) __ pair. a. A=C b. A=T c. G=C d. B=Q e. T=T Can you also explain it please so if I'm ask
The left atrium is a thin walled cavity. Most of the wall is smooth. Musculi pectinati are present only in the auricle of the atrium. The interatrial septum separates the cavity of
Write about 25 parasites of protozoans? And writ the class family and full history about these parasites?
There may also be non-use existence values for components of biological diversity due to the value placed on biodiversity purely based on its continued existence, irrespective of w
Q. What is the function of the right ventricle? To where does the right ventricle pump the venous blood? The venous blood is carried as of the right ventricle to the lungs by t
The Waldorf family was caught in a fire but escaped. Unfortunately, the father and daughter suffered burns. The father had second-degree burns on his entire chest, abdomen, and bot
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