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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What are the most important worm infections? The most significant human worm infections are taeniasis, schistosomiasis, cysticercosis, ascariasis and ancylostomiasis (hookwo
Q. What are the blood vessels that carry venous blood to the heart? What is the blood vessel that collects arterial blood from the heart? The blood vessels that debouch in the
Fibro-osseous Retention V/S Osseointegration Fibro-osseous retention is the tissue to implant contact interposition of the healthy, dense collagenous tissue between the implant
can u show the different activities on the topic
Q. List the steps of mitosis and briefly describe what happens in each.
Define needs of Vitamins arid Minerals in Postoperative Nutritional Care? Vitamins are required for wound healing after a surgery. Vitamin C provides the cementing material of
Exterrral and Internal Environment Let us try to understand the concept of environment with some examples. Can you identify the environment of a single carp fish in the pond? It
Term Translation or protein synthesis, is quite similar in eukaryotes and prokaryotes. We will look at the details of the process in prokaryotes and consider the differences in euk
Define the Criteria for Assessment of Thiamin Status? Thiamin status has been assessed by measuring urinary thiamin excretion under basal conditions or after thiamin loading, t
Q. Cardiac Catheterization of mitral stenosis? Cardiac catheterization is rarely needed to diagnose mitral stenosis. An end diastolic gradient more than 5 mm Hg. across mitral
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