Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

J-shaped growth curve, J-shape d - It shows 2 phases - (i) Lay ph...

J-shape d - It shows 2 phases - (i) Lay phase (ii) Exponential phase

Define methods of food preservation, Define methods of Food Preservation? ...

Define methods of Food Preservation? Food preservation is one of the oldest technologies used by human beings. The perishable food materials like fruits, vegetables, milk, meat

St depression only on recovery, Q. ST Depression only on Recovery? Alth...

Q. ST Depression only on Recovery? Although ST depression during exercise often persists into recovery, it may not manifest until exercise has been terminated. The reasons for

Lungs, what is the lungs structure in relation to the excretory system?

what is the lungs structure in relation to the excretory system?

#biology tutor title.., When nd how i can srt my online work in this site q...

When nd how i can srt my online work in this site question #Minimum 100 words accepted#

Fluid mosaic model, FLUI D MOSAIC MODEL Proposed by Nicholson and ...

FLUI D MOSAIC MODEL Proposed by Nicholson and Singer (1972) Proteins and lipids are arranged in a mosaic fashion. Lipids and proteins are in a semi fluid structure.

What is the connection between tissue fluid and plasma, What is the connect...

What is the connection between tissue fluid, plasma and lymph? Tissue fluid is plasma (minus its proteins) which has leaked out of the capillaries. Lymph is tissue fluid which

Define metabolic response to food - requirement of energy, Define Metabolic...

Define Metabolic response to food - Requirement of Energy? Eating requires energy for the ingestion and digestion of food, and for the absorption, transport, inter conversion,

Why protein requirements of athletes get increased, Why Protein requirement...

Why Protein requirements of athletes get increased? Protein requirements of athletes, particularly those engaged in strength and power events, may be increased above those of s

Skeletal system - cerebro-spinal fluid, Cerebro-spinal Fluid - Formed m...

Cerebro-spinal Fluid - Formed mainly by choroid plexus and ependyma of ventricles. It comes out of medulla oblongeta by foramen of magandie and lushka . Slightly alkalin

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd