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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is the second polar body? After termination of the second meiotic division of the oocyte II two cells are generated: the second polar body and the egg cell proper. The
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Explain the Penicillium - Fungi? Classification Kingdom - Mycetae Division - Amastigomycota Class - Ascomycetes Subclass - Plectomycetidae Order - Eurotiales
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Define the Utilization of Most Probable Number Techniques? The MPN technique (multiple tube technique) can be used to determine the presumptive coliform count in the food. The
Microorganisms and Plants can synthesize all of the 20 standard amino acids. Mammals, furthermore, cannot synthesize all 20 and must obtain some of them in their diet. Those amin
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Describe the Planes of Muscles The planes of superior and inferior recti in primary position form an angle of about 23 0 with the Y-axis. Therefore, the axis of rotation of th
Benefits of Exercises Benefits of exercises for a diabetic patient are given below: - Improves sugar control. - Decreases HbA1C level. - Decreases blood pressure. -
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