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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Name few polymers in the eukoratic cell? Name the mummers that make up the DNA? Compare and contrast between mummers of DNA VS monomers of RNA?
The initial evaluation of new onset heart failure should include an electrocardiogram, chest radiograph, and B-type natriuretic peptide assay. The cardiac rhythm may be normal sinu
difference btn massflow and pressure law
Determine the importance of Educational History- assessment A complete school history includes information regarding a child's current grade placement, any grade repetitions o
Theodosius Dobzhansky and Boris Spassky demonstrated the working of normalising selectioion a behavioural trait in two populations of Drosophida pseudobscura. The two populatiors w
basic difference beyween gymnosperm,angiosperm and pteridophytes.
Estimation of Burn Area: The estimation of the extent of burn area expressed as the percentage of the surface area of the skin burnt to the total body surface
explain cell cycle
Gene expression must begin with the process of transcription a) Describe the promoter motifs commonly associated with eukaryotic protein-coding genes, and explain their influen
Explain the Pre Pregnancy Weight and Foetal Outcome? Several studies from around the world from developing and developed countries have demonstrated an association between pre-
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