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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
15 character of protozoa
Group specificity Some enzymes prefer a specific functional group to be present\%on the substrate molecules. Example:- alcohol dehydrogenase acts on alcohols having -OH gr
What STRUCTURAL features do these three organisms have in common in terms of a) excretory systems b) respiratory systems c) circulatory systems and d) nervous systems ? The species
Main successful vaccines are live attenuated and heat killed vaccines.
Cidofovir (Vistide) IV cidofovir given once weekly for 2 weeks and then once every 2 weeks for maintenance therapy can delay progression of CMV retinitis in patients with AIDS
Phototropic curvature is the result of uneven distribution of : 1. Gibberellin 2. Phytochrome 3. Cytokinins 4. Auxin Auxin
Oxygen is a major component of all living organisms. All the organisms and plants required oxygen for aerobic respiration which they absorb from environment. Only plants release ox
E n z y m e - linked immunosorbent assay (ELISA): ELISA in its var io us modifications e.g. plate ELISA, sandwich ELISA, competitive ELISA, ELISA strip or
Freezing Point - Properties of Solutions The freezing point of a material is the temperature at which it changes from a liquid to a solid. A liquid freezes when its vapour pre
which bone forms the non-moving muscle attachment in the hip joint
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