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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the Culture Media and Its Types? A culture medium (Pl. media), we already know, is a solid or liquid preparation containing all the nutrients required by microorganisms
Natural Classification Natural classification is based on the natural characters of the taxa. Some consider natural classification a phylogenetic one reflecting the evolutionar
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History of diet nnd or tube feeding tolerance History of diet nnd or tube feeding tolerance: The dietitian presents the patient's history, which may include presence or hi
Which of the following is composed of ONLY glucose monomers? (mark all that apply) Select one or more: a. Starch b. glycogen c. Maltose d. chitin e. Cellulose
Q. Why is a leguminous crop rotation used in agriculture? The Leguminous crop rotation and other crop rotations are used in agriculture for the reason that in these plants many
Define and compare bone modeling and remodeling Bone modeling is a surface-specific activity (apposition or resorption) that produces a net change in the sie and/or shape of a
Explain the process of diagnosis of cholera Diagnosis: Cholera can be confirmed only by the isolation of the causative organism from the diarrheic stools of infected individual
Explain Turnover number Turnover number:- The number of molecules of substrate transformed per catalytic site of the enzyme per minute,
Q. What is biotic potential? The Biotic potential is the capability of growth of a given population under hypothetical optimum conditions, i.e., in an environment without limit
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