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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
An enhance in parasympathetic discharge to the heart leads to A. An enhance in the conductance of F-channels in SA node cells. B. An enhance in the conductance of potassium
Explain the Obligatory Losses of Nutrients? Obligatory losses of nutrient are defined as 'the losses that occur when an individual is put on a diet free of that nutrient'. For
Sexually-transmitted diseases Many travelers have new sexual contacts during their journeys and are at increased risk for acquiring sexually transmitted diseases including HIV
As the name shows, the cytoskeleton is responsible for the supporting of the normal shape of the cell; it also acts as a facilitator for substance transport across the cell and for
Q. SARS is a disease that appeared in 2003 with epidemic features in the province of Guangdong, in east China. What kind of agent causes SARS? SARS is caused by a virus from th
What are the cells that form the cartilaginous tissue? The main cells of the cartilages are the chondrocytes, originate from the chondroblasts that secrete the intersticial ma
Explain Procedure for the test of Presence of Coliform Bacteria? This test procedure is in continuation to the presumptive test procedure followed on last experiment. Now carry
Considering the hybridization in a trait like the color of the flowers of a given plant species (red/yellow) conditioned by a pair of different alleles in relation to complete domi
Medical Management Trealment of choice is cephalosporin which is given interavenously, alternatively can be given. In case of increased intra cranial pressure mannitol and o
Community Change Ecosystems are dynamic entities in which a number of events take place. Associated with the biotic communities of the ecosystem are some changes, which may be
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