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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is antistaphylococcal prophylaxis and pseudomonas aeruginosa? I''m doing a project on cystic fibrosis, and I''m curious what these terms have to do with the disease. Thanks!
Define Phenolic Acids and Derivatives? Two families of phenolic acids are widely distributed in plants: a range of benzoic acid derivatives and those derived from cinnamic acid
Binding of Pigments, synthetic dye In addition to water, lipids and volatile flavours, food proteins can bind a number of other substances by weak interactions or
Define the Structure of D and L isomerism? The prefix D or L are used to refer to the configuration of the carbon next to the primary alcoholic group or in other words, the con
Aves has a nucleated rbc or not???
whats the meaning of asexual reproduction
LIGHT MICROSCOPY (LM) A study of the structure of organisms is an essential requirement of biology. However, unaided human eye cannot see objects smaller than 0.1 mrn(100 p) in d
Please answer the following question on Sequence Y: Protein databases 1. What sequence from which organism is this sequence most similar to? 2. Can you find any homo
Biological Stress Since in nature, the various organisms do not live in complete isolation from others, stress to a plant species might also be caused by what other organisms
Q What are some examples of tissues and organs where mitosis is more frequent, less frequent or practically absent? Generally in vertebrates mitosis is more frequent in tissues
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