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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
is the leukamia dangerous
Cell body in a neuron, the part which contains the nucleus and most of the cytoplasm and organelles. Cell cycle It is the sequence of events from one division of the cell to
Q. What are the enzyme cofactors? Some of enzymes require other associated molecules to work. These molecules are known as enzyme cofactors and they can be, for example, organi
Class Arachnida of Phylum Arthropoda Body divided into cephalothorax and abdomen. Cephalothorax along with four (4) pairs of legs; abdomen segmented or unsegmented, with or wi
explain why expired air contain co2
TB in pregnancy Treatment of TB should be initiated in pregnancy when there is moderate to high suspicion of disease because active infection during pregnancy poses a risk to t
1. Many bacterial genes show adaptive regulation of their transcription. a) How is the lac operon regulated by the sigma factor? b) How is the lac operon regulated by lactose
Explain about Regenerative therapy Is also used to achieve the above objectives but with the ultimate goal of regeneration of lost bone tissue.
What are the main functions of (a) the basal (Malpighian) layer, (b) the cornified layer of the skin? (a) The basal (Malpighian) layer makes new skin cells and the pigment
Why is there a concern on waste water?
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