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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
The process of fatty acid elongation is similar to fatty acid synthesis as it also requires both: -malonyl CoA and NADPH -malonyl CoA and NADH -Acetyl CoA and NADH
what is ascus ?
Soil Mixing The larger soil organisms are responsible for considerable mechanical mixing and weathering of soil. The wedge-like action of roots and other underground plant orga
virus symmetry
Signify the term - Proglottids? Serially repeating segment like structures found in tapeworms which contain reproductive organs. Immature proglottids are continually added at t
Q. What are the major harms caused by vitamin A deficiency? How does this vitamin act in the physiology of vision? Deficiency of vitamin A (retinol) may corneal dryness (xeroph
Regeneration Leading To Asexual Reproduction Regeneration is described as the replacement of the lost parts of the body of an organism. This capacity is present in approximat
Causes of Degradation of Ecosystem Due to Agriculture Degradation of the ecosystem due to agriculture can be on the following accounts: a) Removal of trees (deforestation
Explain Biochemical investigation Biochemical investigations: These help to reveal nutrients and metabolites in blood and /or urine, and/or faeces that indicate an infectio
A snapdragon plant that bred true for white petals was crossed to a plant that bred for purple petals, and all the F1 had white petals. The F1 was selfed. Among the F2, three other
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