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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Red-green colour blindness is X-linked recessive. A woman with normal colour vision has a father who is red-green colour-blind. The woman has four sons, none of whom are colour-bli
explain industrial melanism and genetic repatterning during isolation with example
Explain the Obligatory Losses of Nutrients? Obligatory losses of nutrient are defined as 'the losses that occur when an individual is put on a diet free of that nutrient'. For
Carbohydrates are the next important nutrients as they have to perform a variety of functions such as: 1) Provide major source of energy 2) Breakdown of fatty acids and preve
Define the Food Sources of Thiamin? Thiamin is present in many food products and depending on the amount of vitamin present, we have categorized the foods as rich, good or fair
Define Advantage & disadvantage of using Algae as source of protein? Advantage Produces proteins which have almost all the Essential Amino acids. Rich in tyrosine
Induction in Metamorphosis Some of the morphogenetic changes throughout metamorphosis are found fo be quite independent of hormone action. For instance, generally during meta
Q. Illustrate the Important rules of nomenclature? Nomenclature is allied to taxonomy as it deals with the determination of the correct name to be applied to a known taxon. The
Q. Definition of Osseointegration in microscopic biology? From a view point of macro and microscopic biology and medicine. Osseointegration of a fixture in bone is defined as t
Conjugated Proteins Conjugated proteins are composed of easy proteins combined with a non- proteinous substance. The non-proteinous substance is known as prosthetic group o
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