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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Freezing Point - Properties of Solutions The freezing point of a material is the temperature at which it changes from a liquid to a solid. A liquid freezes when its vapour pre
Japanese encephalitis Japanese encephalitis (JE) is a mosquito-borne encephalitis and is caused by flavivirus belonged to the family Flaviviridae. It is a zoonotic disease, i
F A TT Y ACIDS They are monocarboxylic organic acids (R.COOH) having a hydrocarbon chain of 4 - 30 carbon atoms. The polar carboxylic group is hydrophilic (Gk. hydro
Genes and Alleles The inheritance of any character can be studied only when thcre are two contrasting conditions, such as yellow versus green seed colour (as observed by Mendel
Heifers As efficient reproductive performance is essential for economic livestock production, the female calves must grow rapidly to attain sexual maturity, ovulate and be mated b
ARE BONY FISHES AMMONOTELIC?!
Q. Can the heat capacity of water be taken small or large ? What is the biological importance of that characteristic? Ans. From Thermology it is define that the quantit
Explain different modes nutrition like Autotroph,hetrotroph,saprotroph,holozoic
Define the Causes of Failures of Root Canal Treatment 1. Errors in diagnosis and treatment planning 2. Coronal leakage ( inadequate restorative protection) 3. Lack of kno
Determine the Testing for Albumin in Urine Acetic Acid Test About 2 - 8 mgm/dl of protein is excreted in urine which is not usually detected by routine test. The testing o
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