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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Defense mechanisms used in Diabetes Mellitus? Defense mechanisms are the strategies to cope with the real situation and to maintain self-image. Healthy persons normally use
Explain Water - An Essential but Overlooked Nutrient? You may already know that the total body water (TBW) constitutes 50-60% of the body weight. A 70 kg 'standard male' contai
Tularaemia The disease is known by different names like deer fly fever, rabbit fever, Ohara’s disease or tularensis. It is an acute, febrile, granulomatous infection, caused b
Q. If a fragment of nucleic acid has a nucleotide sequence TAC can one assert that it is an anticodon or a codon? A nucleic acid having a TAC sequence surely is not tRNA it is
Blastulation explain
using either nested brackets as a cladogram, what are the hierarchical relationships among the followingtaxa:raniates, amniotes, mammals, actinopterygians, chondrichthyes, aves, ve
Bone Remodeling Bone remodeling differs from the other means of bone structure alteration in that osteoblasts and Osteoclasts do not act independently but are coupled and bone
Approaches to Neuropsychological Assessment Probably the most useful model of neuropsychological interpretation is described in Reitan and Wolfson's (1993) four approaches to a
GRE stands for the Glucocorticoid Response Element: The binding site in a promoter to which the activated glucocorticoid receptor can be bind. The glucocorticoid receptor is signi
Q. What are the functional divisions of the nervous system? Functionally the nervous system can be divided into the visceral nervous system and somatic nervous system. The s
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