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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is risk factor interaction ? Coronary artery disease, as has been explained, is a multifactorial disease with diverse risk factors coming together and interacting to produ
Syngamy and Triple Fusion After traversing through the stylar region, the ultimate destination of the pollen tube is to reach the female gametophyte and release the male game
Explain Free Water - Water Found in Food? Water which is bound by such minute forces, that its vapour pressure is equal to the vapour pressure of pure water. It can be found as
Why acquired traits are not directly related to the process of evolution? As acquired traits are not genetically determined, they cannot be passed on to offspring. Thus, they
P e s t e des petits ruminants It is also known as goat plague or kata resulting in high fever, diarrhoea and respiratory distress. In India, the disease was reported in th
Use of micro-organisms to control insect pests, pathogens or weeds constitutes biological control, and the biological agents employed are called biocontrol agents. Micro-organis
discuss about protozoa,metazoa,mesozoa and parazoa
Types of polyembryony
STRUCTURE Golgi body consists of :- (1 ) Cisternae These are plate like unbranched structure with dilated ends. One golgi body has 4-8 cisternae. Mono cisternal
Neuroglia - Organisation of Nervous System We may refer neuroglia as the connective tissue of the nervous system. It involves all elements of the nervous system other than ne
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