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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the primary function of blood? The primary function of the blood to transport oxygen from the lungs to body tissues for interior respiration. The blood helps in maintai
diseases of the large intestine
Which of the following best describes the tenants of Pangenesis Theory? A. The hereditary material is composed in every organ/tissue and is transmitted to the next generation b
Define Discovery of other Essential Nutrients? Following the identification of the food factors involved in beriberi, pellagra and scurvy, the search was on for other water-sol
Define Most Probable Number (MPN) Method? The MPN method is like agar shake tube method where no agar is used. The dilutions of the microbial suspension are made in liquid medi
What are the examples of Law of Minimum?
EGG S OF INSECT This eggs is centrolecithal and it is elliptical. It diameter is 2-3 mm. It has two eggs membrane i.e. vitelline membrane and chitinous capsule. The chit
Explain about the Dietary Reference Intakes? Dietary Reference Intakes (DRIs) are relatively new to the field of nutrition. The DRIs are a set of four nutrient-based reference
Endothelium is a source of vasoconstrictor factor also. The best characterized of these are the endothelins. Endothelium produces only ET1. Unlike NO, which is released rapidly in
PAEDIATRIC NURSING Parent education, family health promotion and health maintenance have been the concern of yesterday's and today's nurses. Florence Nightingale over a hu
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