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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
why should breathe faster after a hard work
Enumerate about the diseases Insomnia The results from studies of people, who claim that they do not sleep, or wake up frequently from sleep show that their insomnia can have m
Explain the Fistulative surgery - Endodontic Surgery = (Incision and drainage) 1 Cortical trephination 2 Decompression 3
P a r a typhoid and other Salmonella infections Paratyphoid salmonellae are non-host-specific. The commonly reported species are S. Typhimurium, S . Enteritidis S . T
Many endangered plant species are currently being propagated by commercial growers and botanical gardens and the sold (as both plants and seeds) to government agencies. Conservatio
When a cell is respiring aerobically, which two gases are likely to be diffusing in and out of the cell, and in which direction will they be diffusing? When a cell is res
fat digestion requires two steps, what are the steps and what enzymes are use to accomplish
How Vitamin K used to Prevents bone loss? Vitamin K is known to inhibit bone loss through inhibiting effect on osteoclast formation. Thus, adequate levels of vitamin K must be
Indications For Surgery : The normal aortic valve area in the adult is about 3-4 cm2. Significant symptoms occur when the valve area is seduced to 1/4"' the normal area
Therophytes - Classes of Life Form These are annual plants of the summer season or of the favourable season. They complete their life cycles in a single favourable season and
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