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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is the mechanism described by Darwin that eliminates species less adapted to environmental conditions? The mentioned mechanism is the natural selection.
MICROTUBULES Discovered by De Robertis and Franchi . Term given by Slautterback . These are hollow structures, consists of tubulin protein. Each protein diamer
DISORDER S OF FEMALE REPRODUCTIVE SYSTEM - (i) Sterility (ii ) Menstrual irregularity - This may be amenorrhea (absence of M.C.) or excessive bleeding (
diseases of the large intestine
What is the function of the skin in humans? The skin is the external covering of the body. In humans its major functions are protection, perception of information from the envi
Which of the following statements about hemoglobin and myoglobin structure are true? Select the five true statements. 1. By itself, heme is not a good oxygen carrier. It must be
A plant grown from one of Mendel's yellow pea is selfed. Three progeny peas from this are obtained and they are all green. Is the original plant homozygous or heterozygous, and wha
Explain about the Solar Dryers? There are so many different designs for solar dryers, these range from simple convection dryers, cabinet dryers, shelf dryers to more complicate
Enumerate about the Baule unit A mathematician from Gottingen by the name of B. Baule assisted Mitscherlich with his calculations. The quantity of any growth factor (nutrient
The life of a eukaryotic cell can be explained as a cell cycle. Mitosis and cell division happens in the M phase that lasts for only about 1 h. This is followed by the G1 phase whe
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