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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
EGG S OF INSECT This eggs is centrolecithal and it is elliptical. It diameter is 2-3 mm. It has two eggs membrane i.e. vitelline membrane and chitinous capsule. The chit
What is the purpose of DNA? Actually, DNA makes you who you are. It has all your heredity info, like your hair color, your personality, etc. No two person's DNA is the similar,
Which disease is associated with the following symptoms? Sudden onset of profuse watery stool followed by vomiting, rapid dehydration and muscular cramps?
Assessment This includes the data collection (nursing history and physical assessment), comparison of data with the normal, and analysis of the data gathered. Systematic an
VERTEBR A T A It is divided into two parts - 1. Agnetha 2. Gnetha S.No. C haracter A gnetha G
Several TCA cycle intermediates can be used as a substrate for the synthesis of other compounds. Succinyl CoA is on intermediate that is directly used for the synthesis of which of
POR T AL CIRCULATION When a vein branches into set of capillary to distribute blood in some parts, such venous circulation is called portal system. Three types of portal syst
Q. Explain about Rheumatic Heart Disease? Rheumatic Heart Disease (RHD) is a very common cause of cardiovascular disorder in children and adolescents in India. This disease inv
A climate classification system divides regions according to _____?
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