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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Perception of Light - Floral Induction Stimulus Leaves are found to be organs that perceive light 'stimulus', K.C. Hamner and J. Bonner showed in 1938 that even if all the lea
Define Supplementation to prevention of iron deficiency anaemia? Supplementation with low doses of iron is necessary to prevent anaemia in particular groups of people. Fortifie
Into which subphyla are the protochordates divided? What are some representatives of each protochordate subphylum? Urochordates (or tunicates) and cephalochordates are the two
If an enzyme-controlled reaction normally takes place at 10ºC, in general terms how will the reaction be affected by (a) a fall in temperature to 2°C , (b) a rise in temperature t
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how respiration takes place in mammls and reptiles
Fredrick Griffith published a series of experiments that collectively were dubbed "The Transforming Principle" in 1928. Which of the following stopped him from being able to declar
a) What does secondary productivity in an ecosystem indicate? b) List any two factors by which productivity is limited in aquatic ecosystems
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