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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is connective tissue proper? The name connective tissue proper is used to designate the connective tissue that fills interstitial spaces as opposed to the specialized c
a) Mention the main cause of air pollution in metro cities. Write any three ways by which it can be decreased. b) How did Eli Lilly synthesize the human insulin? Mention
Assume that the blood volume of a rabbit is 0.5 liters. If the effective dose of the anesthetic urethane is 10 micrograms/mL, calculate the volume of a 25% urethane solution that m
what is the mode of nutrition in pisces
What are the plant organs responsible for the perception of light variation? What is the pigment responsible for this perception? Leaves are mostly responsible for perception o
a) How is the milk production regulated by hormones in human female? Define. b) Explain the importance of the auxin / cytokinin ratio in plant tissue culture.
What do you understand by Heterotrophic? Organisms which are not capable of converting light into chemical energy. They should consume other organisms or material produced by o
DEVELOPMENTAL DISORDERS - 1 . AMNIONITIS- Inflammation of amnion due to infection. 2 . ABORTION- Escape of a embryo prior to the stage of viability (ab
W a s t e Management in other species Solid and pellet nature of sheep and goat faecal matter make it easier to handle. This manure can easily be swept off the floors and d
Anton van Leeuwenhoek was the first to propose placing microorganisms into the Kingdom Monera. True or False
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