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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are the main water properties that make water special for life? The water properties that make water biologically significant are molecular polarity, thermal stability (el
What is Active Transport--Secretion This method is now a well accepted process of aqueous formation. The rate of aqueous humour formation depends on the rate of active solute
Which of the following statements is correct? A. Hemoglobin binds O2 more tightly at pH 7.2 than at pH 7.4. B. Fetal hemoglobin under physiological conditions binds O2 more t
VITAMIN - D Also known as Calciferol / Anti ricket vitamin / sun shine vitamin. It is necessary for bones & teeth. It is synthesized in presence of UV rays of sunl
Q. Carbohydrates requirements for ulcerative colitis? Carbohydrates: They form the easily absorbable source of energy. Bulk-producing vegetables are restricted so as to allow b
Foot examination For all diabetics, foot examination is important and necessary in all visits to identify risk factors of ulcers or injury of foot. This will help in preventing
Nutrient Budgets - Nutrient Cycles Nutrients are constantly being added and removed by natural and artificial processes. The measure of the input and outflow of nutrients thro
DIFFERENCE S BETWEEN AXON AND DENDRITE (DENDRON) AXON DENDRIT E (DENDRON) 1. It is single per neur
Chlorophyll Chlorophyll is the porphyrin most widely used as a natural colourant. Its widespread occurrence in photosynthetic tissue and its breakdown character have prompted
What is phosphorylation? What are some biological processes in which phosphorylation plays a critical role? Phosphorylation is the name given to processes of the addition of ph
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