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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Looking at what the academic world is publishing, microarray research is actually hot right now, and people haven't quite figured out what the best way is (some might argue if we s
Birth Marks (Naevi): Birth Marks is also known as naevi. It is a multiplication of one or more cutaneous elements within the skin. It is defined as a congenital, ci
Impoundments - Lentic Ecosystems We have so far discussed natural lakes. In addition to these there are a number of lakes both small and large artificially created by man cal
Q. What are the major theoretical models that try to explain the formation of the enzyme-substrate complex? There are two major models that explain the formation of the enzyme-
Explain about the Importance of Vitamins? Vitamins are the organic substances that act as coenzyme and/or regulator of metabolic processes. There are 13 known vitamins, most of
In the fruit fly Drosophila, a rudimentary wing called "vestigial" and dark body color called "ebony" are inherited at independent loci and are recessive to their dominant wild-typ
Or nit h o s i s (psittacosis) This is an important zoonotic bacterial infection and causes disease in humans and birds. Collectively, these conditions are called as chla
Determine Guidelines for Planning Balanced Diets for Elderly? The key element in planning diets for the elderly should be nutrient-based dietary guidelines, in order to reduce
Define the term- Adaptation The terms scotopic and photopic vision are relative to the lighting conditions of an individual place. Light adaptation is a very quick process and
Define Objective of national programmes targeting infants? These programmes have the objective of hinging down under-5 mortality. Under Integrated Child Development Services (I
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