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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How brain thinks?
Where is the gall bladder located? The gall bladder is located in the Upper Right Quadrant (URQ) of the abdomen just below the liver. It keeps bile secreted by the liver. The d
Q. Is fecundation in amphibians internal or external? In this aspect are amphibians evolutionarily proximal to fishes or to reptiles? In the majority of the amphibian species f
Explain Protostomes vs. Deuterostomes breifly? Branching Evolutionary Lines : It appears that the Coelomate animals separated into two divergent lines of evolution in terms o
List three types of evidence used by systematic taxonomists to construct phylogenetic diagrams. Types of evidence contain the morphology of fossil and living species, patterns
what is biological sickness of soil and its management
defination of phyletic lineage?
Explain about common congenital heart diseases ? Congenital Heart Disease is commonly classified as acyanotic and cyanotic. Acyanotic Left to right shunts Obstructive Lesion
Pyruvate carboxylase is a mitochondrial matrix enzyme whereas the other enzymes of gluconeogenesis are situated outside the mitochondrion. Thus oxaloacetate are produced by p
Pinocytosis: - The ingestion of dissolved materials by endocytosis. The cytoplasmic membrane invaginates and pinches off placing small droplets of fluid in a pinocytic vesi
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