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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Which of the following serves as a controlled variable in a negative feedback system? A. Levels of hydrogen ions in the interstitial spaces of the brainstem. B. Blood plasma
What are the properties og Gels? Polysaccharides, proteins or colloidal complex particles, such as caseinate micelles form gels at levels of 10% or less. Gums, pectin and gela
Q. What is commensalism? The Commensalism is the ecological interaction in which one individual benefits while the other is neither benefited nor harmed. The Commensalism is a
Starch exists in plants as insoluble starch granules in chloroplasts. Each starch granule holds a combination of two polysaccharide forms, amylopectin and amylose.
what is biological sickness of soil and its management
Gastrointestinal System The purpose of digestion is to change the food by chemical action and convert it into simple form to be utilized by various tissues. The process of dige
advantages and disadvantages of protozoa
Q. Meaning of Gestational Diabetes Mellitus? In this type of diabetes, increased blood sugar level is first diagnosed or recognized during pregnancy. Women are not known as dia
Which are the brain regions associated with memory? According to researchers some of the main regions of the nervous system associated with the memory phenomenon are the hippoc
Q. Microorganisms on On basis of nutrient degradation capacity? On basis of nutrient degradation capacity: - Proteolytic: Microorganisms, which are capable of protein degra
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