Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What is the structural depiction of a carboxyl group, Q. What is the struct...

Q. What is the structural depiction of a carboxyl group? Carboxyl groups have a carbon attached to one hydroxyl group by a simple bond and one oxygen by a double bond. The othe

Explain about the connecting models and data, Explain about the Connecting ...

Explain about the Connecting models and data? Perhaps the most dramatic change in quantitative environmental biology in recent years has been the burgeoning progress in the dir

Medical management of meningitis , Medical Management Trealment of ch...

Medical Management Trealment of choice is cephalosporin which is given interavenously, alternatively can be given. In case of increased intra cranial pressure mannitol and o

What do you understand by carcinogens, Q. What are carcinogens? The Car...

Q. What are carcinogens? The Carcinogens are factors capable of producing neoplasias and any mutagen, a substance that can induce DNA mutation, is a potential carcinogen. Insta

Explain the importance the relationship of structure, Explain the importanc...

Explain the importance of understanding the relationship between structure and function?

Various modalities available for sterility assurance, Q. Enlist the various...

Q. Enlist the various modalities available for sterility assurance? Various modalities available for sterility assurance are a) Prevention of overloading b) Chemical indi

Interkinesis, There is no Interphase preceding second meiotic division. ...

There is no Interphase preceding second meiotic division. There is a brief intervening period called Interkinesis During this period there may be synthesis of some reserve fo

Diversity in living organisms, which organism are called primitive and how ...

which organism are called primitive and how are they different from the so-called adavnced organisms

Explain the decolourizing agent - ziehl-neelsen method, Explain the Decolou...

Explain the Decolourizing Agent - Ziehl-Neelsen Method? Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allow

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd