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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define briefly about the Cereal Proteins? Cereal grains, properly ripened and dried for optimum storage stability, have protein content ranging from 6-20%. Proteins are found i
Proto-oncogenes Proto-oncogenes are common genes present. In all organisms and emerge to be highly conserved in the course of evolution. They have been isolated in species ra
Explain about the Vitamin A? Vitamin A, one of the fat soluble vitamins, refers to a sub-group of retinoid that possess the biological activity of all-trans-retinol. The term '
List three ways that DNA technology could be used to improve the lives of humans. Answers could include: identifying human remains, determining a person's paternity, tracing h
1) The presence of epicardial coronary artery stenosis caused by artherosclerotic plaques is by far the most frequent angiographic finding in any cardiac ischaemic syndrome. 2)
The fragility of erythrocytes The fragility of erythrocytes is impaired in the absence of NADPH generation due to the deficiency of glucose-6-phosphate dehydrogenase thereby ca
general character and classification of platyhelminthes
Bacteriophage Vectors Bacteriophages are viruses that attack bacteria. Mainly phage lyses the bacterial cells they infect (lytic phages). But many others may choose to follow e
Factors that affects Oxygen Dissociation Curves - Temperature At higher temperature haemoglobin gives up oxygen more readily and the dissociation curve shifts to the right. Th
Zinc deficiency Zinc is essential for several physiological functions in the body such as protein synthesis, carbohydrate and nucleic acid metabolism, and foetal growth. The de
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