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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain about the Vacuum Evaporators? It is common to construct various vacuumised vessels in series that is why the product moves from one vacuum chamber to the next and thus
The non-oxidative phase generates ribose precursors The non-oxidative phase of the pathway, including the following reactions, converts pentose-5-phosphate to other sugars.
How are lipids classified?
Composition of blood: Take few ml. of fresh blood in a test tube and before collecting the blood add a few drops of sodium oxalate or sodium citrate solution to prevent clot
Define Defensive Proteins - Biological Functions of Proteins? These proteins protect in opposition to diseases. Antibodies are the instance of defensive proteins. These combat
green gland is excretory organ of which ortopodic phylem
Family Centred Activities to Ensure Harmonious Relationship: Between various members of the family attitudes of mutual trust, love and respect for one another need to be fo
Q. What is the major cell organelle involved in cell digestion and what are the properties of that organelle that enable it to do the task? The organelles accountable for intra
A woman who is heterozygous for a particular X-linked recessive trait marries a phenotypically normal man. What percentage of their sons will show the recessive phenotype?
Name the Foods to avoid in soft diet include: Fried foods and nuts, rich pastries and desserts, Raw vegetables, Heavily spiced foods, Gas-forming vegetables, S
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