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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Which type of virus is the HIV? What is the enzyme reverse transcriptase present in HIV? HIV is a retrovirus that is RNA viral (its genetic material is RNA and not DNA).
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Define Functions of Copper? Copper serves as a co-factor, as well as, an allosteric component of enzymes. In many enzymes copper functions as an intermediate in electrno transf
Q. Which Of the substances is chromatin made? Chromatin is made of the DNA molecules associated to proteins known as histones.
List a few applications of starches in the food industry. A few applications of starches in the foods industry include thickener, a fat sparing agent, adhesive, binder,
Gastrulation in Amphibians Amphibians comprise a large and moderately telolecithal egg. Cleavage is holoblastic and unequal generating a spherical blastula along with a reduc
Explain Micafungin sodium Micafungin sodium (Mycamine - Astellas), the second echinocandin antifungal1 to become available in the US, has been approved by the FDA for intraveno
Define Impediments to infuse biology - Lack of trained people? At present, biologists as a rule have not been trained sufficiently in quantitative approaches. A heavy dose of m
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What is an evolutionary tree? Is there a precise evolutionary tree known by science that explains the emergence and origin of every type of living being? Evolutionary tree is t
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