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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
The biologicaI species concept claims that species consist of natural populations and that species are real and objective. They are not man-made subjective abstraction. According
Indications for Surgery : Once diagnosis of constrictive pericarditis is made and confirmed by chest X-ray, ECG, echocardiogram, CT, MRI scan, cardiac catheterisation and angio,
Explain about the water-soluble vitamin - Riboflavin? Riboflavin (vitamin B 2 ), a water-soluble vitamin, was discovered in milk as a pigment possessing a yellow green fluore
The main causes for the rapid growth in the quantity of solid wastes are- (i) Over population: the ever increasing population is increasing all types of pollution; same is t
Define about Skeletal system Skeletal system provides framework to the body; supports the soft tissues; protects delicate organs. The skeletal system is made up of bones an
Define the Management of Epileptic Patients? The primary treatment of epilepsy is anticonvulsant drugs. The focus of nutritional management is a diet, which is appropriate for
Describe Alternation of Generations? Alternation of Generations : In meiosis, four haploid daughter cells are formed from one diploid mother cell. The life cycles of sexuall
how many arrs are present in mataphasic telocentric chromosomes
Defone Economic consequences of malnutrition? Figure explains the economic consequences of malnutrition. You would note from the Figure that the economic productivity of the in
Q. What is the respective importance of water, carbon and nitrogen for living beings? Water is the major solvent of living beings and it is necessary practically for all bioche
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