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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Where does poop come from?
Impoundments - Lentic Ecosystems We have so far discussed natural lakes. In addition to these there are a number of lakes both small and large artificially created by man cal
Explain the Failure to Management of Ledge Apical transportation or perforation: movement of the physiological foramen to a new iatrogenic location. Three types of apical tr
What is Pancreatic lipase Pancreatic lipase acts on the dietary triacylglycerols after it has been incorporated into the mixed micelle in the intestinal lumen. The lipa
Explain about colloidal system A colloidal system, on the other hand, is a heterogeneous system. The material that forms the base of the system is called the dispersion medium
Which of the branches of applied biology ?
Define Muscular endurance component for physical fitness? It is the ability of a muscle, or a group of muscles to sustain repeated contractions or to continue applying force ag
What is antistaphylococcal prophylaxis and pseudomonas aeruginosa? I''m doing a project on cystic fibrosis, and I''m curious what these terms have to do with the disease. Thanks!
Determine the functional brain activity PET is powerful means of assessing functional brain activity, although it does not directly measures neuronal events. Rather it indicate
Annuloplasty with a Ring : The lings commonly used are Carpentier- Edwards and Cosgrove's flexible half ring. The rings are C shaped and have incomplete circles to accommoda
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