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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
S m o k in g of Meat Products The purpose of smoking meat is to develop a distinctive flavour, aroma and appearance. These attributes can also be achieved with liquid smok
Carbohydrates are the next important nutrients as they have to perform a variety of functions such as: 1) Provide major source of energy 2) Breakdown of fatty acids and preve
Q. How do arthropods grow? Due to the presence of exoskeleton the growth of an arthropod is periodical during the growth period the animal loses the exoskeleton, develops and g
notes on butterfly
Define the Recommended Dietary Allowance of Vitamin E? The requirements for the vitamin E are expressed in terms of tocopherol equivalents (TE) as mentioned earlier - 8 mg fo
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Q. What are changes in ST-Segment? Normal Exercise Electrocardiogram ST and J Point As the heart rate increases with exercise, a number of predictable changes occur in a
Assume you were given a mixture consisting of one molecule each of all possible sequences of a smallish protein of molecular weight 4800 daltons. If the average molecular weight of
What are muscle tissues? How is the function of this tissue related to the typical characteristics of its cells? Muscle tissues are tissues made of cells able to perform contr
Q. In which areas of the globe is gymnosperm abundance noteworthy? These plants are the typical vegetation of cold regions like the boreal forest or taiga of the northern hemis
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