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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Dietary fibre Dietary fibre: Typhoid patient has an inflamed intestinal mucosa, which can be easily perforated and ulcerated leading to internal haemorrhage. Thus foods hig
Why are most ammoniotelic beings aquatic animals? Aquatic animals, like crustaceans, bony fishes and amphibian larvae, usually are ammoniotelic since ammonia diffuses more easi
what is exonephric
Explain the Characteristics of Agar- Agar? There are many characteristics, which make agar-agar, an ideal solidifying agent. These are: (a) It has high gel strength, so it c
C dna is made from a mature m rna of eukaryotic cell with the utilization of enzyme called as reverse transcriptase. Steps in c dna creation 1. m rna is made and purified f
Define Functions of fluoride? The only beneficial role demonstrated for fluoride is in reducing the prevalence and severity of dental caries in children and adults. This is en
What is the primary structure of a protein? What is the significance of the primary structure? The primary protein structure is the linear sequence of amino acids that form the
What is Polymerase Chain Reactionin genetics? A great advance in sequencing and cloning DNA is the polymerase chain reaction, devised by the American biochemist Kary Mullis in
Explain the Procedure of Taking Blood Pressure - Explain the procedure to the patient. In case patient is coming for first time to check the blood pressure explain to the patie
Q. Inhibition of microorganisms in food? Microorganisms use our food supply as a source of nutrients and energy. They increase their numbers by utilizing nutrients. This can re
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