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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the Advantage of Sponge Method? Advantage: The method is suitable when the surface to be swabbed is large or low incidence of microbes in the environment.
Study of Plsmodium
Q. What are the pentoses? What are the vital roles of pentoses in DNA and RNA molecules? Pentoses are carbohydrates whole of five carbons. The DNA molecule is made of a sequ
Cell body in a neuron, the part which contains the nucleus and most of the cytoplasm and organelles. Cell cycle It is the sequence of events from one division of the cell to
1. Awareness - First people become aware of a problem. 2. Acquire Knowledge and skills - Next, they gather knowledge and learn new skills. 3. Motivation - At the next stag
Give a short explanation of what UPS really means and why it is so important to health practitioners and consumers/patients.
What is the difference between octopuses and mussels regarding their circulatory systems? How does that difference influence the mobility of these animals? Cephalopod molluscs,
Pyruvate carboxylation Pyruvate carboxylation : In this reaction, pyruvate, CO, and ATP are converted to oxaloacetate, ADP and Pi catalysed by the enzymepyruvate carboxylase
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Ventricular arrhythmias are usually produced by excess catecholamines and vagal withdrawal and occasionally re-entry and triggered activity also plays a role. PVCs are more common
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