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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Miscibility in binary liquid systems? When two different pure liquids are unable to mix in all proportions, they are said to be partially miscible. When these liquids ar
Nutrition As we have said earlier all animals are heterotrophs and require food from the environment. What is this food made up of? If the food of a number of different animal
1. Effect on general health: Noise pollution increases the rate of heart beat, constriction of blood vessels and blood pressure. Noise also causes anxiety, stres
Define Categories of Indicators of Malnutrition? The indicators may fall under the following categories: 1. Macro indicators for sector-wide monitoring and evolution, 2.
ALL ABOUT AUTOCHORY?HOW IT WORKS
Q. What is Cardiac Catheterization? Routine cardiac catheterization is not necessary in patients with mitral regurgitation. It may be done when there is discrepancy between cli
Construct a genomic DNA library with the help of some methylaee and finall do viral packaging.
Soil pH and Nutrient Availability Soil pH is the most important factor which governs the availability of nutrients in soil. All the nutrients are absorbed by plants in their io
Q. What is Malabsorption Syndrome? Did you know that a major part of the absorption of nutrients takes place in the small intestine and the set of enzymes involved in this proc
what are the modes of nutrition in different animals
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