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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Anticoagulant therapy To prevent blood clotting on the valve, patients with prosthetic valves require life long anticoagulant therapy. The patient is put on oral antico
P a ck a g i n g of Meat Products Meat needs to be packaged to prevent contamination, color deterioration, loss of moisture, odor pickup, oxidative rancidity etc and pro
diversification and economic importance
Food Applications of Carrageenan Carrageenan consists of a family of hydrocolloids, which have different properties and it has a wide variety of uses. Some examples of properti
Coronal Disassembly - If the existing restoration with marginal defect: - The crown was sectioned through the bucco-occlusaly , Christensen crown remover elevate and remove
Factors Limiting the Productivity of Aquatic Habitats Sunlight and oxygen are the two most important limiting factors of the aquatic ecosystems. This distinguishes them from t
ASDd
Long ago, there lived an Australopithecus girl by the name of Lucy. She died 3.2 million years ago. There are a few molecules of Lucy’s carbon in YOU! Starting from the gaseous C
Bacillary Dysentery: You have learnt about the diarrhoea in the foregoing sub-section, now let us take for example a child who has loose motion which contains blood and mucus
In the previous sub-section we discussed the possible ways by which variabiliti can, be generated. We shall now examine one instance that illustrates the consequence of variability
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