Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Human impact on nitrogen cycle , Human Impact on Nitrogen Cycle Human...

Human Impact on Nitrogen Cycle Human activities are profoundly affecting the cycling of nitrogen in nature. Over 30 x 10 6 metric tons/yr. of N 2 is fixed in the commercial

Sublimation-evaporation-transpiration-water cycle , Sublimation is the pr...

Sublimation is the process by which solid water changes directly to vapour phase without passing through the intervening liquid phase. The gradual disappearance of flakes of ice

What are the applications of nicotinic acid, What are the Applications of N...

What are the Applications of Nicotinic acid Nicotinic acid is mainly used in the vitamin fortification of flour, macaroni and noodle products. Independent of its vitamin effic

What is a genotype, What is a genotype? What is the difference between geno...

What is a genotype? What is the difference between genotype and phenotype? Genotype is the genes, DNA nucleotide sequences have in the chromosomes of an individual that conditi

What is biosynthetic pathways, The intermediates in the cycle give precurso...

The intermediates in the cycle give precursors for various biosynthetic pathways. An instance is as given below: -  From citrate synthesis of fatty acids; -  Amino acid synth

Nutrition in animals, filter feeding mode of nutrition is observed in- a) a...

filter feeding mode of nutrition is observed in- a) amoeba b) euglena c)paramoecium d) all of the above

Explain how the boll weevil populations changed, The boll weevil, an insect...

The boll weevil, an insect native to Mexico and Central America, is another pest of commercial cotton. When chemical insecticides were first discovered, they proved highly effectiv

What are the main representatives of the pteridophytes, What are the main r...

What are the main representatives of the pteridophytes? Is this plant group cryptogamic or phanerogamic? The better known pteridophytes are the ferns and the maidenhairs, from

Psychology, Psychology : This is the study of human mind. On other words Ps...

Psychology : This is the study of human mind. On other words Psychology is the science of behavior or mental processes. Its immediate aim is to understand the individuals or groups

Define management of canal blockage canal calcification, Define Management ...

Define Management of Canal Blockage Canal Calcification Diamond coated instrument rt 1 designed for better visibility and cutting efficiency a) Very powerful for efficient

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd