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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the Quebec Colony Counter? It is used for counting the number of colonies in a plate. Culture plate is kept on the screen which is illuminated from beneath. Counting is
Question 1. State the basic properties of light. Discuss the special features of ultra-microscopy and phase contrast microscopy 2. What are ultrasonic waves? Explain the dif
what arr the economic importance of phylum protozoa
Q. Carbohydrates requirement in chronic diarrhoea? Adequate amount of carbohydrates i. e 60-65% of the total energy should be given to the patient. Easily assimilated carbohydr
Moss Stage - Xerarch The accumulation of soil, particularly in the crevices and depressions of rock favours the growth of certain xerophytic mosses, e.g., species of Polytrich
what are the general characteristicss of porifera
what is the deference between sp. and spp.?
History of Psychiatric Nursing: In 1873, Linda Richards, developed better nursing care in psychiatric hospitals, and organized nursing services and educational programmes in
1. You are observing adsorption of a virus to the host cell, it uses long leg-like structures, what are they called?
Explain the Uses of ISO in Seafood products Seafood products The most significant application in this category is the use of ISP in fish sausage and surimi based restructu
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