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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain assessment of iron status - Transferrin receptors? Transferrin receptors: As iron deficiency progresses into second stage, the number of transferrin receptors on the ce
What is the Determination of vitamin B 2 In the chemical method, the yellow-green fluorescence of aqueous solution of riboflavin is measured. In the microbiological assay, tur
What is Oral Hygiene An index can be used to evaluate the oral hygiene level in the patient e.g. Oral hygiene Index (Green and vermilion) or Plaque index (Silness and Loe). An
Water is considered a universal solvent because- Select one: a. it can dissolve polar and non-polar compounds b. it can dissolve both positively and negatively charged ion
Equipment and Supplies: Nurses who are aware of location of equipment and supplies do give accurate delivery of patient care without delay and wastage of time and energy for
Define Maternal Dietary Intake and Foetal Outcome? Among the various nutrients, energy intake appears to be the most crucial, for developing countries. Studies from India have
What are angiosperms, the flowering plants? What is the major feature that distinguishes them from the gymnosperms? Flowering plants have flowers and seeds (phanerogamic plants
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How is extracellular digestion related to cellular and tissue specialization? Several of specialized cells and tissues appeared with extracellular digestion to give enzymes and
Explain about colloidal system A colloidal system, on the other hand, is a heterogeneous system. The material that forms the base of the system is called the dispersion medium
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