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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Determine Materials for Separation of Amino Acids by Paper Chromatography? Petridishes (200 mm diameter) with lid, Whatman Chromatographic paper, brown paper, 500 ml separating
Simultaneously with the formation of two daughter nuclei, a cleavage furrow appears in the middle of the cell. The furrows gradually deepen and divide the cell into two daughter ce
principle behind seliwanoff''s test
Fowl spirochaetosis The causal agent Borrelia anserina is a spiral-shaped organism about 8-30 um in length but commonly 14-15 um long and 0-3 um wide. The organisms are activel
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Classification of coelentrata upto sub classes
Excretory organ of crustaceans and agama lizard
What are the chemical elements that form most of living biological matter? The elements are:- a) oxygen (O), b) carbon (C), c) hydrogen (H) and d) Nitrogen (N).
Fensom and Spanner Electroosmotic Flow Hypothesis In electroosmosis the ions flow across a membrane in response to electrical gradient. Ions pull along water and other content
characteristic of spider that makes it the phylum arthropoda?
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