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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What does it mean when a plasmid is cleaved with Hind III and Sca I? Does that mean that it opens up at those two locations and I have to insert whatever is complementary to it?
Define the Goals of treatment of Obesity? As said in the beginning, the goal of treatment of obesity today has shifted from mere 'weight loss' to 'weight management'. Each weig
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Explain transposition with VSD with restricted pulmonary? Transposition of great arteries with VSD with restricted pulmonary blood flow: Restriction to pulmonary blood flow
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As we age, our skin wrinkles due to change in collagen and elastic fibers in the dermis and a decrease in their production. Explain why topical application of collagen and elastic
In eukaryotes, the electron transport and oxidative phosphorylation occurs in the inner membrane of mitochondria. These processes re-oxidize the FADH 2 and NADH which comes
Which type of genetic disease can be identified from the visual analysis of the number of chromosomes present in a karyotype? The counting and the identification of chromosomes
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