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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Some ecosystems possess relatively large number of species as compared to others. This is particularly true of tropical forests which exhibit a very rich-biodiversity. For example,
Define Colorimetric Method - 2, 4 Dinitrophenylhydrazine Method? We shall use this method in estimating vitamin C in a given sample in the laboratory. Here, let us look at the
Q. Explain Hydraulic Formula? This relates to pressure gradient and velocity of flow: V2 = (Cv)2 2gh or V = (Cv) 2gh Wherein, V = velocity of flow Cv = coefficient
What is an argument that shows that the emergence of photosynthetic beings was crucial for life to reach the marine surface and later the dry land? The Ultraviolet radiation fr
Q. Etiologic factor of atherosclerosis? Various factors are responsible for atherosclerosis. These include: 1. Hyperlipidemin: Excess circulating fats in blood especially th
Temperature - Seed Dormancy Low temperature treatment is an essential prelude to germination in many seeds, and high temperature may be inhibitory at the time of germination.
Definition,functions and features of a mammalian lungs
Q. What is parthenogenesis? Parthenogenesis is the formation or reproduction of a new individual from the egg cell but without fecundation by the male gamete. According to the
The following histograms were produced using flow cytometry after labelling B-cell lymphocytes with propidium iodide. Histogram A is from a healthy individual while Histogram B is
Define the symptoms in Bone marrow of pernicious anaemia? The nucleated red cells of the marrow are greatly increased. The successive nucleated cell stages in erythropoiesis a
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