Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Describe the principles of dna sequencing, The ability to sequence DNA has ...

The ability to sequence DNA has led to a revolution in molecular biology. a) Describe the principles of DNA sequencing. b) A single sequencing reaction yields at best 1500 ba

Is gene a triplet of consecutive dna nucleotides, Is gene a triplet of cons...

Is gene a triplet of consecutive DNA nucleotides A gene isn't a triplet of DNA nucleotides with their respective nitrogen-containing bases, like CGT or AAG. The nucleotide trip

What is the function of the citric acid cycle, What is the function of the ...

What is the function of the citric acid cycle? The hnction ofthe  citric acid cycle can be discussed as  follows. The  intermediates of citric acid cycle are used as  precursor

Common equine diseases, COMMON EQUINE DISEASES - 1 .       INFLUENZA ...

COMMON EQUINE DISEASES - 1 .       INFLUENZA OR PINK EYE - Pink eyes. Swallon eye lids. 2.       STRANGLES - Fever, dullness, depression, nasal discharge, lumpy a

Poultry and duck diseases-duck plague, Duck plague Duck plague, caused ...

Duck plague Duck plague, caused by a member of Herpesviridae, has world wide distribution and occurs among domestic and wild ducks, geese, swans and waterfowls. Epidemiolo

How to draw the cell in metaphase, Start with a cell with chromosome number...

Start with a cell with chromosome number 2n=6, and having two acrocentric pairs and one metacentric pair of chromosomes. Draw the cell in metaphase I. Indicate one pair of sister c

Zoology, structural adaptation of mammalian alimentary canal

structural adaptation of mammalian alimentary canal

What are nutritional care related to neurological disorder, What are nutrit...

What are nutritional Care related to Neurological Disorder? Let us then move on to the nutritional care. The important goals of nutritional care are to: prevent further

Hygroscopic coeflicient-retention of soil moisture, Hygroscopic Coeflicient...

Hygroscopic Coeflicient To have a more complete picture of soil moisture relations, let us take a soil sample and oven dry it for 24 hours at 11 o C. Now if this soil is kept i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd