Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Temperate deciduous forest - ecosystem, Temperate deciduous forest - Ecosys...

Temperate deciduous forest - Ecosystem The temperate forests are characterised by a moderate climate and broad-leafed deciduous trees, which shed their leaves in fall, are bar

Determine the physiological effects phytoestrogens, Determine the Physiolog...

Determine the Physiological Effects phytoestrogens? Little is known about the biologic and physiologic effect of phytoestrogens in humans. Animal studies suggest the following

Mechanoreceptors - receptors, Mechanoreceptors - Receptors Mechanorece...

Mechanoreceptors - Receptors Mechanoreceptors involve those receptors involved in perception of touch, pressure, tension, hearing, vibration, gravity, muscle tension etc. Th

Explain structural proteins, Explain Structural protiens Structural pro...

Explain Structural protiens Structural proteins are fibrous proteins. The most familiar of the fibrous proteins are probably the  keratins, which form the protective covering o

Explain about regenerative therapy, Explain about Regenerative therapy   ...

Explain about Regenerative therapy   Is also used to achieve the above objectives but with the ultimate goal of regeneration of lost bone tissue.

Determine the glycogen receptors in the plasma membranes, Which of the foll...

Which of the following serves as an effector, or as part of an effector, that functions in a negative feedback system? A. Glycogen Receptors in the plasma membranes of liver ce

Explain brotherhood and sisterhood using abo blood typing, Is it possible t...

Is it possible to perform investigation of natural paternity, maternity or brotherhood and sisterhood using the ABO blood typing? By using an ABO blood typing it is possible on

Etenobotany, Ethnobotany : This is the study of relationship between tribal...

Ethnobotany : This is the study of relationship between tribals and plants. Ethnobotany is from "ethnology" - study of culture or "botany" - study of plants. This is the scientific

Determine the reliability of the battery, Determine the reliability of the ...

Determine the reliability of the battery The issue of reliability of the battery has been addressed, with reasonably successful results. Clinical interpretation of the battery

Describe about defecation reflex, Q. Describe about defecation reflex? ...

Q. Describe about defecation reflex? An example of a reflex having an autonomic (and somatic) component is the DEFECATION REFLEX: The rectum is generally empty, but when fec

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd