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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
steps involved in glycolysis
Which of the branches of applied biology ?
Q. List the stages of process of behaviour change? Stages of behaviour change process: · Unawareness · Awareness · Acquiring knowledge · Motivation · Trial
Explain Hypertensive Disorders of Pregnancy? Hypertension may have been existing before pregnancy. Alternatively, a mother may develop hypertension during pregnancy, a pregnanc
ROL E OF WATER - In general water constitutes 60-90% of protoplasm. Average 80%. It forms 2/3 of human body weight, i.e. 66.66%. Due to electrophillic nature of O, wa
Kidney - 2 in number. Dark red. Slightly flattened. Bean shaped. 10 cm. long, 5 cm wide and 4 cm thick (In rabbit 20-25 mm). Weighing 150 gm in male 135 gm in fema
Nitrogen is needed mostly for the synthesis of amino acids and nucleotides. Sources of nitrogen can be obtain from organic as well as inorganic sources, but the whole target is the
Kinds of Matter On basis of its chemical organization, matter id of three categories elements, compounds and mixture An element is composed of obviously, earth similar atoms
Define the Intradermal Dye Test? The rate of decolouration of a small quantity of the dye, 2, 6- dichlorophenol injected intradermaly is an index of the ascorbic acid nutrition
In eukaryotes the SER has enzymes able to begin double bonds into fatty acyl CoA molecules in an oxidation reaction which uses molecular oxygen. This reaction is catalyzed
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