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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
CLASSIFICATION OF HABITUATING DRUGS - There are a large number of drugs on which people become dependent. These are classified into 2 main categories - (i) Psychotropic d
Explain the Concept of Menu Planning? You have learnt from the previous units that our body's many functions, be it physical activity, mental exercise, growth (in the case of c
Explain disease typhoid Typhoid is often called enteric fever because the infection or bacteria is found in the intestines and attaches itself to the epithelium of the intes
Q. What is the difference between rough and smooth endoplasmic reticulum? The endoplasmic reticulum is a delicate membranous structure contiguous to the nuclear membrane as wel
Why, even though they have an open circulatory system, can flying insects like flies beat their wings with great speed? In insects the circulatory system is open but this syste
Q. Symptoms of gastro oesophageal reflux disease? Most commonly, people with GERD complain of heartburn, a painful or uncomfortable feeling in the chest, which may radiate to t
Assuming that all human proteins are 300 amino acids long, what fraction of the total number of possible different proteins is found in the human body?
Q. How does the male gamete penetrate the egg cell? How does the female gamete protect itself from the entrance of more gametes after the entrance of the first sperm cell? The
Decomposers - Biotic Components Also known as saprotrophs. Mostly, these are microscopic and are heterotrophic in nature. Decomposer organisms obtain their energy and nutrient
Explain Voriconazole It has a spectrum of activity similar to itraconazole but appears to be more active against Aspergillus spp. And some species of Candida, containing C.
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