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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
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Define Half Saturation Test and Full Saturation Test? This test is specific to polysaccharides. The test is used to detect dextrin from starch. The details related to the test
TEST CROSS Crossing of F hybrid with its homozygous recessive parent is called test cross and the progeny of test cross is called test cross progeny. A . Monohybrid Te
how does sodium and potassium ions maintain the resting petential of neurons
How might stem cells and other tools of "regenerative medicine" be used to treat brain diseases? Fuelled by the hope which stem cells may have the capacity to rebuild virtually
Define Liposuction - surgical management for obesity? Liposuction is a cosmetic surgical procedure different from bariatric surgery. It involves aspiration of subcutaneous fat
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M-Mode was the earliest form of cardiac ultrasound used clinically. It displays the amplitude signals of various structures along the length of ultrasound beam with distance or
Composite Endosperm - Variants of Endosperm In the family Loranthaceae, the development of endosperm is unique. The ovary lacks ovules. The sporogenous tissue located at the b
Cotyledon is a leaf-like structure which is present in the seeds of the flowering plants; it appears during seed germination and sometimes is referred to as the seed leaf.
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