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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Why have biologists and bioinformaticians embraced the Web as a vehicle for disseminating data so quickly, whereas clinicians and clinical informaticians have been more hesitant to
Concerning permeability what type of membrane is the cell membrane? The cell membrane is a selectively permeable membrane, i.e., it permits the passage of water and some select
S T ARC H - Polymer of a-D-glucose. Starch is glucosan homopolysaccharide which is the major reserve food of plants. Starch is formed as an end product of photosynt
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Conduction in Myelinated Axons The myelin sheath provides insulation for the axon preventing movements of Na + and K + through the membrane. Therefore, if this myelin sheath
Advantages of Tissue Valv es Need for anticoagulation therapy is only for a short duration in xenograft and only there is rare need for anticoagulation therapy in au
How Pregnancy and Lactation affects the bmr? These periods of physiological stress also has an impact on the metabolic rate. Earlier, we studied that REE is highest during the
Explain the Dietary Modifications - Obesity? The dietary modifications serve as a guide for the obese to make healthy food choices. The first step towards prescribing a diet fo
The bar-tailed godwit, Limosa lapponica baueri, or kuaka, is a wading shorebird seen on marshy estuaries and wetlands during the New Zealand summer. Populations of this species emb
Valvular heart diseases : Congenital and acquired valvular heart diseases often require surgical intervention. 1 is well known that conservative surgery by valve repair gives bet
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