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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Precipitation or Solubility of Proteins? Most of the functional properties are dependent on the degree to which the proteins are soluble. The solubility behaviour provid
Conduction An action potential occurs at one point along the axon. Yet we know that neurological impulses are not fixed, they travel along a neuron. So how can the action pote
Community Gradients and Boundaries It is often difficult or impossible to determine where one community ends and the next begins. Many communities, in fact, grade continuousl
What is Implant therapy Successful implant therapy requires systematic thorough and meticulous planning to determine the ultimate prognosis and end result that will satisfy th
I stored a few of my things from April to September this year. When I wanted to unpack the boxes, I found hundreds of round, small, black things. They look like they might be snail
Virus envelope is known as: 1. Capsid 2. Virion 3. Nucleoprotein 4. Core Capsid
Why healthy skeletal muscle fiber is isolated A healthy skeletal muscle fiber is isolated and has no external forces on it. It has normal intracellular levels of ATP and is ba
Define the Criteria for Assessment of Thiamin Status? Thiamin status has been assessed by measuring urinary thiamin excretion under basal conditions or after thiamin loading, t
Since the availability of flu vaccine is limited, who should receive any future pandemic flu vaccine?
Density - Population Parameters and Regulation Density is defined as number of individuals or population biomass per unit of area or volume at any given time. Biomass refers t
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