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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How can the enzymes be classified? Explain giving examples. Based on structure, enzymes can be classified into monomeric enzymes and oligomeric compounds. Monomeric enzym
Inorganic Substances - Abiotic Components There are about forty elements that are required in various processes of living organisms. Some of these are macronutrients which the
Active instruments - Do groove occlusally or buccally until reach tooth structure - Apply this instrument then screw to break the bond between tooth structure and crown GC
Define about the Mid-day Meal Programme? The National Programme of Nutritional Support to Primary Education commonly known as the Mid Day Meal (MDM) Scheme (was launched in Aug
Lactation tetany of mares (eclampsia, transit tetany) Hypocalcaemia in mares causes lactation tetany, which is characterized by abnormal behaviour, in coordination and tetany.
cassification of mode of nutrition
What is a stream? A stream is a incessant series of bytes that flow into or out of your program. Input and output from devices like the mouse, , disk, keyboard, screen, modem, a
Which of the following defines why the vast majority of segregation errors in human patients include the two sex chromosomes (XXY, XO, XYY) or chromosome 21 (triple 21)? A. The
Which of the following statements is not true of mitosis? A. The original cell produces two identical daughter cells B. It follows duplication of DNA in the nucleus C. It involves
What is Phylum Platyhelminthes - Flatworms ? This Class consists of the free-living flatworms, the best known of which is the Genus Planaria. Most of the species in this class
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