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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Vitamin C (Ascorbic acid) Vitamin C, ascorbic acid is also called anti-scorbutic vitamin. Ascorbic acid occurs as white crystals or white crystalline powder without odour and o
why is it necessary to grind food before the testng?
Q. What are the three phases into which the HIV infection is often divided? The HIV infection is frequently divided into three phases the acute phase, from the infection to 1 u
Sheep and goats The disease is readily transmissible to human being from goats, and is known as Malta fever, Mediterranean fever or undulant fever. The chief causal agent is B
Just definition
What is the function of the secretions of the prostate, seminal vesicle and bulbourethral glands in reproduction? These secretions with sperm cells from the testicles form the
Which are plant tissues responsible for the supporting of the plant? The plant supporting tissues are the sclerenchyma and the collenchyma. The collenchyma is made of elonga
Determine How to Calculate the Amount of Nitrogen? The amount of nitrogen can be calculated for this value using the equation: 1000 ml of 1N H 2 SO 4 = 1000 ml of 1N NH 3
what is the structure of hiv virus?
A drug has a molec. weight of 200. How many grams would you use to make 100 ml of a solution that is isotonic (for man)? Assume that the diluent is distilled water and that the dru
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