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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Fluconazole Fluconazole is active against most Candida species other than C. krusei, which is intrinsically resistant, and many strains of C. glabrata, which are increasingly
The allele that causes albinism (p) is recessive to the allele for normal pigmentation (P). A normal woman whose father is an albino marries an albino man whose parents are both no
Wind pollinated flowers are: 1. Small, brightly coloured, producing large number of pollen grains 2. Small, producing large number of dry pollen grains 3. Large producing
how do we write a limerick spell?
Synthesis and Storage of Hormone The synthesis and storage of hormone depends on the nature of the hormone. Steroid hormones are secreted in diffuse molecular form and usually
why obelia is of special interest in zoology
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Q. Basis of Classification on Form-relationship principle? As pointed out earlier, this system is mainly based on Form-relationship principle. thus the various genera were. g
Q. How do birds reproduce? Birds like every vertebrate have sexual reproduction. Their embryos develop within shelled eggs containing extraembryonic membranes and exterior the
AMOEBA Systematic Position Amoeba was discovered by Russel von Rosenhoff in 1755. He called it the little proteus after the mythical sea god who is believed to be c
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