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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Basic working of Midbrain? Midbrain: Also known as the mesencephalon, this part is located behind the frontal lobes and in the center of the entire brain. It deals with func
Explain about the Drying - methods of food processing? Drying is the oldest and probably the simplest way of preserving food. It is usually accomplished by the removal of water
Explain Requirements and Recommended Dietary Intake? A very small daily intake of vitamin C (10-15 mg/day for an adult) is required to avoid deficiency and stave off scurvy. Ho
Q. What is the name of the cells capable of making gametes? What is the ploidy of these gamete-forming cells? The cells that form gametes are the germ cells as opposed to the s
Testing for urine sugars is not recommended for either diagnosis or monitoring of patients with diabetes. This is because a urine sugar is not a reliable test. When no facilities a
Infectious proteins are present in: 1.Gemini viruses 2.Prions 3.Viroids 4.Satellite viruses Prions
Why TPP and TTP are vital for the nerves and cardiac tissues? TPP and TTP are interconvertible and arc involved in carbohydrate metabolism. As discussed above, when there is in
Q. Can you explain Listeriosis? Listeriosis is a food borne illness caused by a pathogenic bacterium called Listeria monocytogenes, which is food borne. Listeria monocytogenes
plz give the classificational chart of animals upto speceis level
Class Gastropoda Body asymmetrical, depicts torsion or its effects; shell coiled in most, well developed head with radula, large flat foot, gills one or two or with pulmonary
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