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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Deficiency of vitamin -A (hypovitaminosis-A) Vitamin A deficiency develops due to insufficient supply of the vitamin or its defective absorption from alimentary tract. The defi
Which of the following best compares the results of mitosis and meiosis? Mitosis results in four diploid cells, while meiosis results in two haploid cells. Mitosis results in four
What is Invertebrates? Invertebrates: About 99% of all the animals lack backbones, and are invertebrates! Invertebrates include the phylum Arthropoda, or the animals with joi
Explain how many bones in the human face? Ans) 14 bones are present in human face and these bones are known as sepio
Describe the process of excitation-contraction coupling (ECC) in the heart. How do adrenaline, noradrenaline and acetylcholine alter heart rate and or force?
What is the relation between secretion of parathormone and the calcium blood level? The parathormone enhances the calcium blood level as it stimulates the resorption (remodelat
Explain Inhibitors Inhibitors: Citrate synthase is inhibited by ATP, NADH, succinyl CoA and acyl CoA derivative of fatty acids (fatty acyl CoA). The rate of the reaction is
Chloroplasts Like mitochondria, chloroplasts are also distinctive organelles of eucaryotic cell. But they are found only in plant cells. Chloroplasts contain membranes forming
State the symptoms of epilepsy Three particular symptoms are found in many types of epilepsy: 1) An aura, or warning, of impending seizure: This aura may take the form of a
Define about the Sporocyst Larva? Sporocyst is the second larval stage in the life cycle of F. hepatica. It develops from the miracidium larva within the pulmonary chamber of
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