Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Causes of degradation of ecosystem due to agriculture, Causes of Degradatio...

Causes of Degradation of Ecosystem Due to Agriculture Degradation of the ecosystem due to agriculture can be on the following accounts: a) Removal of trees (deforestation

What main idea, What key idea, contained in Malthus's essay on populations,...

What key idea, contained in Malthus's essay on populations, helped Darwin formulates his theory of natural selection?

Why is the amazon rainforest under risk of desertification, Despite having ...

Despite having a great biodiversity why is the Amazon Rainforest under risk of desertification? The natural soil of the Amazon Rainforest is not very fertile but it is enriched

Mechanisms for response – protozoan, Mechanisms for Response – Protozoan ...

Mechanisms for Response – Protozoan Protozoa are sensitive to many kinds of stimuli like touch, temperature changes, light, chemicals, etc. It is not clear how they do so but

Give the introduction to congenital heart disease, Give the introduction to...

Give the introduction to Congenital Heart Disease ? The past 3 or 4 decades have witnessed a revolution in pediatric cardiac sciences. A number of conditions previously conside

Why is sub-culturing essential in plant tissue culture, Why is sub-culturin...

Why is sub-culturing essential in plant tissue culture? Explain the process of heterosis. How is it dissimilar from inbreeding depression? Where and how is urea produced in

Explain about stacking faults, Explain about Stacking faults Stacking ...

Explain about Stacking faults Stacking faults happen in a number of crystal structures, but the common example is in close-packed structures.  Face-centered cubic (fcc) struct

Respiration, Respiration It is important that you comprehend the main ...

Respiration It is important that you comprehend the main points because they ate essential for your understanding of respiratory physiology. To enter or leave the body, oxygen

Illustrate the stages of contact osteogenesis, Stages of Contact Osteogenes...

Stages of Contact Osteogenesis Osteoconduction: This is the first and the most important healing phase and relies on the recruitment and migration of osteogenic cells to the

Proteins of plant origin - nuts, Proteins of Plant Origin - Nuts Nuts a...

Proteins of Plant Origin - Nuts Nuts are excellent sources of proteins. Examples of nuts include cashew nuts, almond nuts , hazel nuts , coconuts, walnuts, brazil nuts ,cashew

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd