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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. How do calcium ions participate in muscle contraction? Why do both muscle relaxation and muscle contraction spend energy? In the muscle cells calcium ions are stored within
Diversity is defined as the no of species present in a community termed as "species richness". We can represent species biodiversity at different geographical scales as alpha, beta
The type of gene interaction in which effects of one gene override or mask the effects of other entirely different genes is known as: a) Mutation b) Linkage c) Pleitropy
Q. Since among the 64 codons of mRNA 61 codify amino acids that form polypeptide chains what are the functions of the three remaining codons? Since there are 20 amino acids and
The chances of transmission in female is mor than males
How is a genetic trait determined by the genetic code within a DNA molecule? A DNA molecule has 4 dissimilar bases, either CGTA. Any specific combination of these things forms
function of epithelial tissue
Explain Positive Staining Technique? Here, a stain has a positively charged chromophore that gets attached to the negatively charged outer surface of the microbial cell and thu
Q. Explain Percutaneous Interventions Over the last two decades, significant strides have been made in the field of Balloon Valvuloplasties both in terms of technique as well a
In this 1st step, the corresponding aminoacyl-tRNA for the second codon binds to the A site by codon-anticodon interaction. Binding of the aminoacyl-tRNA needs elon
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