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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
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What are the reasons of Food Processing? The reasons for food processing may vary, but the main objectives are to: Preserve the nutritive quality of food by preventing th
what is entero coelom
What are the principal sources of excessive nitrate and phosphate in rivers and lakes?
Q. Definition of Consciousness? Consciousness is a phenomenon that is shared by nearly all people. It cannot be directly seen or touched. Yet it is real enough to most people.
Explain the Quebec Colony Counter? It is used for counting the number of colonies in a plate. Culture plate is kept on the screen which is illuminated from beneath. Counting is
Poisoning: Poisoning is a common medical emergency in childhood. In children under 5 years of age essentially all poisoning are accidental. Nearly 75 per cent of all poi
Restricted Food Items Foods to be avoided by diabetic patient are carbohydrate and fatty food. Carbohydrates are classified into sugars and starches. As you have studied in und
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Discuss FASB and IASB. Comment on at least two specific aspect of the two standard setting boards and their role in setting accounting.
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