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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Guanine is one of the nitrogenous bases in the nucleic acids, guanine is one of the two purine bases.
Q Which is the biological molecule that holds the genetic information that is transmitted hereditarily and controls the cellular functioning? The hereditary molecule that contr
Maintain nutrition Your responsibility as a nurse is to take care of nutrition and prevent the child from starvation. Breast feeding'should be continued during the diarrhoea e
explain protozoa taxonomy
In the previous sub-section we discussed the possible ways by which variabiliti can, be generated. We shall now examine one instance that illustrates the consequence of variability
What is the clinical manifestation of the disease known as daltonism? An X-linked daltonism is a disease in which the affected individual sees the red color as green or confoun
Pesticides - Danger to Wildlife Another recently realised danger to wildlife in many parts of the world has come from the development of more effective pesticides. As agricult
Embrylogical Devlopment of Hypospadias The development of the penis and scrotum is intluenced by the testes. In the absence of the testes, the female structures of the
Q. What is the intermediate host of Schistosoma mansoni? Where does that host live? The intermediate host of a schistosome is a gastropod mollusc, Biomphalaria genus and a snai
Effects of Cardiogenic Pulmonary Edema Interference with oxygen transfer in the lungs Depression arterial oxygen tension Sense of suffocation and oppression in the
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