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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is the phototropism? The Phototropism is the movement of plant structures in response to light. The Phototropism may be negative or positive. The Positive phototropism is
Osmoregulation in Aqueous Environments You are aware that the aqueous environments are of two types: i) Freshwater and ii) Seawater. The osmotic concentration of thes
STRUCTURE OF WATER It may interest you to know that water is a universal solvent and is a major constituent of all living organisms. Earth is the only planet where water exists i
Q What are the three periods into which interphase are divided? Interphase is the preceding phase to the mitotic division. It is divided into three periods, G1, S and G2 the le
Drawbacks of Free Food Items for diabetics patients In this context, it is important to note that a patient can have unlimited servings of 'free foods'. These are foods which d
Which of the following leads to the formation of atherosclerosis plaques? -uptake of oxidized LDL particles by macrophase -non specific interactions between LDL particles
What is Electron Cloud Model ? Electron Cloud Model : In recent models of the atom, electrons form electron clouds, in which electrons travel in spherical and dumbbell-shap
write about the asexual reproduction in mucor
What are the Maximum Crop Yields Mitscherlich conducted a large number of experiments with different plants, making use of pots 20 cm in diameter and 20 cm in depth. By addin
In order to define growth limit logistic equation was given by Verhulst. In a given ecosystem, the maximum population that can exit is called carrying capacity (k). The factors wh
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