Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Morphology of angiosperms, define inflorescence and explain in detail about...

define inflorescence and explain in detail about it''s types

Oxidation reduction potential, what exactly is oxidation and reduction pote...

what exactly is oxidation and reduction potential and how its an imp. factor in spoiling meat

What are the three main parts of a eukaryotic cell, The eukaryotic cell can...

The eukaryotic cell can be separated into two major portions: the cell membrane that divides the intracellular space from the outer space phisically delimiting the cell; the cytopl

Define structures in a vertebrate with a four-chambered, Which of the follo...

Which of the following structures in a vertebrate with a four-chambered heart would have blood with the highest oxygen concentration? And why?

Pdf, study of phenomena of apical dominance

study of phenomena of apical dominance

Assuming that the two traits assort independent, In holly, serrated leaves ...

In holly, serrated leaves are dominant to smooth-edged leaves, and red berries are dominant to green berries. Two holly plants heterozygous for leaf edge shape and berry color are

Energy flow - ecosystem, Energy Flow - Ecosystem Our world is a solar-...

Energy Flow - Ecosystem Our world is a solar-powered system, and green plants are the entry gates of energy into ecosystem. The total incoming solar energy, only a very small

Explain the mechanical requirements of implant materials, Mechanical requir...

Mechanical requirements of implant materials Dental implant s must be able to sustain and transfer loads -It should have adequate mechanical strength in order to distribute th

Nerves - organisation of nervous system, Nerves - Organisation of Nervous S...

Nerves - Organisation of Nervous System Due to the pattern of distribution of neurons as described above, there are bundles of nerve fibers, called nerves, connecting the cent

Can you illustrate period of meiosis, Q. What is the crossing over? And In ...

Q. What is the crossing over? And In which period of meiosis does this event occur? Crossing over is the eventual exchange of chromosomal fragments among homologous chromosomes

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd