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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Advantages of Direct Microscopic Count? 1. It is easy, inexpensive and relatively quick method. 2. It gives information regarding size and morphology of microorganism
Explain Evidence of coverage of Ecology Evidence of coverage of Ecology and Evolution to the level of Descriptor 2 and an evaluation or discussion that shows depth of underst
What is Congenital Mitral Stenosis ? The onset of symptoms or signs depends on severity of the stenosis, severe the stenosis earlier the presentation. With significant MS, the
what is ecology?sub divison of ecology?
Molluscus - Hormones in Growth and Reproduction We know quite well about the endocrine mechanism of growth and reproduction in the freshwater snail Lymnaea stagnalis. This is
Q. Which are the most important inorganic molecular substances for living beings? The most important inorganic substances for living beings are mineral salts, water, molecular
When do you use the term 2' carbon and when do you use 2 carbons on a molecular structure? I need examples please!
Insulin Concentrations The doctor always prescribes insulin dosage in units. Thus all types of insulin (crystalline, NPH, Lente, and so forth) is available in 10-ml vials that
What are the three main types of RNA? What is meant by heterogeneous RNA? Messenger RNA, or mRNA, transfer RNA, or tRNA, and ribosomal RNA, or rRNA, are the three main kinds of
Growth Phase -Stages in spermatogenesis Growth phase is characterized by the acquisition of the structural and functional characteristics of distinct sex cells. Also here is a
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