Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Define the biochemical analysis, Biochemical analysis A basic metabolic...

Biochemical analysis A basic metabolic panel measures sodium, potassium, chloride, bicarbonate, blood urea nitrogen (BUN), magnesium, creatinine, and glucose. It also sometimes

Why do fats have thermal isolation properties, Why do fats have thermal iso...

Why do fats have thermal isolation properties? Triglycerides are weak heat conductors and in addition they form thick layers of fat tissue when accumulated by the organism. Tha

Contractile vacuole - protozoans, Contractile vacuole - Protozoans The...

Contractile vacuole - Protozoans The contractile vacuoles may differ in complexity in various groups of protozoans. In amoebae the vacuoles are carried around in the cytoplasm

How does the body defend itself from microorganisms, Q. How does the body d...

Q. How does the body defend itself from microorganisms and other harmful substances that enter the airway during the breathing process? The epithelium of the airway is a ciliat

Pathology of aortic regurgitation, Q. Pathology of aortic regurgitation? ...

Q. Pathology of aortic regurgitation? In Post Inflammatory situations scarring and retraction of valve cusps lead to aortic regurgitation. Varying degrees of commissural fusion

Is protein collagen has a high proportion of glycine, The protein collagen ...

The protein collagen has a high proportion (20% or more) of A. asparagine and glutamine B. proline and hydroxyproline C. glycine D. both A and B E. both A and C F

Define water of hydration & bound water- water found in food, Define Water ...

Define Water of hydration and Bound Water - Water Found in Food? Water of hydration: This moisture is chemically bound to the constituents of the material and in most cases w

Explain procedure for test the presence of sugar in honey, Procedure for Te...

Procedure for Test the Presence of Sugar in Honey? 1. Mix 5 grams of honey with 5 ml ether in a mortar and pestle. 2. Decant off the ether extract into an evaporating dish.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd