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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
State about Electric Field Lines Electric Field Lines (lines of force) - show direction of force on a positive test charge from a distribution of charges. The electric field
Phylum Chlorophyta (green algae) 1) The chlorophyll is the main photosynthetic pigment. 2) There is little or no cell differention in thallus. Thallus is usually filamento
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Which of Mendel's postulates can only be demonstrated in crosses involving at least two pairs of traits? a) Segregation b) dominance/recessiveness c) independent assortment d) unit
different applications of microbiology
How will the urine concentration be affected if the henle''s loop of a person is removed and his PCT is joined directly with DCT?
TYPES OF PARTHENOGENESIS - 1 . NATURAL PARTHENOGENESIS - In many animals natural parthenogenesis is common process & is a method of reproduction. It is of two typ
Classification of agro-industrial byproducts Based on their nutrient content agro-industrial byproducts can be divided into: 1. Feed low in fiber and low in protein. These ar
Describe the term Humidification Dry gases result in the loss of ciliary activity of the mucosa of the airway and can lead to mucosal damage as well as causing the secretions
characters of phylum protozoa
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