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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How is litmus paper and testing for acid, base, and neutral, related to biology?
If a person has a stomach ulcer (that is aggravated by excessive hydrochloric acid {HCl} secretion ), why on earth would he or she be drinking milk to feel better, when milk is als
Extruded Starch Cereal products, that are prepared by pumping cereals through a small opening for instance, pasta, Ready-to-eat cereals etc.
how can petorl formed
Explain the Virtual Dissection of the Rat in multimedia tutorials? This animation will teach you about basic mammalian anatomy. The major organ systems in a rat are very simila
Define Evolution and Growth of Sports Nutrition as a Discipline? An academic discipline is basically a formal body of knowledge discovered, developed, and disseminated through
Zoonotic diseases and trade Disease and trade have a long-standing, interwoven history. During the 14th century, Europeans realized the value of exotic nature of goods and mat
Classification of hydrocolloids by function It must be clear to you now that gum ghatti, gum karaya, gum tragacanth ( classified as exudate gums) locust bean gum, guar g
Collection of Cerebro-spinal Fluid (CSF) Examination of the CSF is one of the most useful laboratory procedures in neurological diagnosis. Four types of punctures ma
Aim: To prove that Carbon dioxide is essential for photosynthesis. Apparatus: A potted healthy plant with long and narrow leaves, a wide mouthed glass bottle, split cork. Chemicals
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