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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What are the main cytoplasmic structures present in animal cells? The main cytoplasmic structures of the cell are the centrioles, the cytoskeleton, mitochondria, peroxisomes
Chymotrypsin Chymotrypsin is secreted in an inactive form 'chymotrypsinogen', which is activated by trypsin. Chymotrypsin is specific for peptide bonds containing unc
Microevolution is: the change in allele frequency in a population. Cannot occur if genetic equilibrium is reached. Results in the formation of a new species. Is influenced by mutat
Phototherapy As you know exposure of the jaundiced infant to blue, white or green light is effective in reducing the serum billirubin level. When bilirubin absorbs light, ser
Somatic Embryogenesis The process of embryo development is called embryogenesis. It is not the monopoly of the egg to form an embryo. Any cell of the female gametophyte (Embr
Classify phylum mollusca?
Q. What is the spinal cord? Of which elements is the spinal cord constituted? The spinal cord is the dorsal neural cord of vertebrates it is the part of the central nervous sys
describe food procurement and digestion in ciliates.
State the Goals of neuropsychological assessment The psychological domains/processes/ components are mediated by specific brain structures and connected brain structures formin
Define Precautions for estimation of vitamin c in a solution? 1. Rinse all glassware with 3% metaphosphoric acid before you begin your practical and subsequently each time you
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