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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
MOUT H - It is as transverse slit. It is pseudo type i.e. not open directly into alimentary canal. Mouth is covered by upper & lower movable lips. Movement is due to arb
Which part of the carbon cycle is responsible for the enhancing concentration of carbon dioxide in the atmosphere. The burning of fossilized products (coal and oil) of some pla
Carbohydrates Fifty five to seventy per cent of the required energy in animals is derived from carbohydrates. However, fats and proteins can also be broken down and used for
explain the mode of nutrition in paramecium, euglena and hydra
Sex Determination - Reproduction Sex, whether an individual will be a male or a female is determined at fertilisation, and this directs and controls all the later processes i
Respiratory system of Crustacea ?
What is the meaning of Food Exchanges Food exchanges mean grouping of foods in which the specified amount of all the food provides approximately equal amounts of carbohydrate,
Some species of plant are strongly adapted to pollination by certain insects. Characteristics which are regarded as adaptations to pollination by bees are: ( a) white o
Define Biodiversity and ecosystem stability? Human's have transformed ecosystems all through the world, frequently replacing diverse natural ecosystems with ecosystems which ar
Hypospadias Hypospadias is the most common congenital anomaly of the penis. In this uretheral opening is situated on ventral side of the shaft of penis in one or several posi
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