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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. According to the cellular organization how are living beings divided into two groups? Cellular beings are divided into two groups, unicellular beings and the prokaryotes who
Describe the monomer unit of a protein and how monomer units are assembled into peptides.
C o m p o s t i n g It is a modification of a conventional or solid manure handling system with anaerobic decomposition. Types of composting systems include windrow, b
what is genes
H yd r o p e r i c a r d i um - h e pa titi s syndrome Also known as 'Angara disease' or 'leachy heart disease', this complex illness of poultry spread rapid
Phases of Water The state of water can be changed from gaseous to liquid and liquid to solid and vice versa by the addition or removal of heat energy. To convert one gram of li
What is adaptive convergence? The Adaptive convergence is the phenomenon by which living beings facing the same environmental pressure (problems) and undergoing genetic variabi
NUCLEUS First described and christened by Robert brown 1831, a nucleus is the largest and most important organelle of eukaryotic cells. It is membrane bound and generally locat
Q. Which are the three hormones that participate in the regulation of the renal function? Antidiuretic hormone or ADH or vasopressin, atrial natriuretic factor (or ANF) and ald
Andalusian chickens may be either black, white, or gray. The gene for black is not dominant over the gene for white, nor is the gene for white dominant over the gene for black. Whe
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