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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
classification of virus
Food Chain The land mass, water bodies and atmosphere are the three endowments of nature on which the living beings have to depend for their nourishment and sustenance. The
Nuclear transport is an energy-dependent process mediated through saturable receptors. Export and Import receptors are by to distinguish and bind to nuclear localization signals or
SEMEN - Sperms and secretion of accesory glands collectively known as seminal fluid or semen. It is milky, semi-solid in nature having particular smell. pH : 7.35 - 7.5. Spe
Difference between Reproduction System of man and rabbit - S.No. Man Rabbit 1. 2. 3.
What is the Objectives of Neuropsychological assessment? Define and describe neuropsychological assessment; Elucidate the neuropsychological methodology for assessing i
What are some diseases or genetic abnormalities caused by dominant genes? Why are severe dominant genetic diseases rarer than recessive ones? Instance of dominant genetic disea
Why is ATP known as the energy currency of body?
Frequency - Synthetic Characters This term refers to the degree of dispersion of individual species in an area, and is usually expressed in terms of percentage. Frequency can
Define about the Carbohydrates - nutritional biochemistry? Carbohydrates, you should observe by now, are made up of carbon, hydrogen and oxygen only with hydrogen and oxygen at
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