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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
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Bacteria never have a nucleus but some members of the domain Archaea are known to contain a true nucleus.
Define the Composition of Potato Dextrose Agar? This media is used for cultivation of yeasts, fungi and moulds. Potato (Peeled) - 200.0 gm Dextrose - 20.0 gm Agar - 15
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If the results of an experiment turn out differently then you expected, what does that mean? A. The experiment was a failure B. You did not follow scientific method C. You should e
Q. What is Salty taste? Various ions both cations and anions are responsible for the salty taste. These include: K (Potassium), Na (Sodium), Li (Lithium), Cl, Br (Bromine), I (
Action potentials include changes in the membrane potential of an axon, as shown in the schematic below. During the refractory period, sodium channels are inactivated and
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