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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is the difference between xylem and phloem? Both xylem and phloem are vascular tissues found in a plant. Xylem is a tubular structure, which is liable for water transport
Define about the Chemotherapy - Cancer? Chemotherapy results in lot of side effects. This is because the drug effects are not specific to cancer cells alone. Even the host cell
what is the life cycle of a protozoa
Describe the term Epinephrine? Epinephrine is administered IV in increments of 1 mg diluted in 10 mls of normal saline (1:10,000). It can be given through the endotracheal tube
Define the objectives in the biochemistry of the eye. After going by this section, you should be familiar with: a. Dynamic of vascular supply of eye; b. The cornea metabo
Minerals and Trace Elements Oxygen, carbon, hydrogen and nitrogen are the most common elements that make up 96% of the total weight of a mammal. The next most abundant element
Transcription continues until a termination series is reached. The most common termination signal is a GC-rich region which is a palindrome, followed by an AT-rich sequen
Complete history taking is an initial step in diagnosis. You will ask the patient about present complaints, past history, family history, any drug allergy and also the kind of diet
Linolenic Acids Fats in this group contain substantial amounts of linolenic acid (C18 triunsaturated). Examples are soybean, mustard, rapeseed, flaxseed and wheat germ hemps
what is primitive fungi?
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