Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Define the coenzyme activity of nicotinic acid, Define the Coenzyme activit...

Define the Coenzyme activity of nicotinic acid? Nicotinamide exists within the redox-active coenzymes,  nicotinamide adenine dinucleotide (NAD) and its phosphate (NADP), which

Agarose gel electrophoresis, Agarose gel electrophoresis  is the method to ...

Agarose gel electrophoresis  is the method to analyze the size of the DNA (or RNA) fragments. In the presence of an electric field, bigger fragments of DNA move through a gel slowe

Explain disease typhoid, Explain disease typhoid Typhoid is often call...

Explain disease typhoid Typhoid is often called enteric  fever because the infection or bacteria is found in the intestines and attaches itself to the epithelium of  the intes

Forebrain - diancephalon, DIANCEPHALO N - Less visible. Occupies on...

DIANCEPHALO N - Less visible. Occupies only 1% of brain's volume. Its lumen is diocoel or III ventricle. Roof is epithalamus . On it pineal body is present. Fl

Respiration in cockroach, what is the chemical reaction of respiration in c...

what is the chemical reaction of respiration in cockroach?

What are the products and reagents of fermentation, Q. In general what are ...

Q. In general what are the products and reagents of fermentation? In fermentation glucose sugar is degraded into pyruvic acid each glucose molecule forms two pyruvic acid molec

What is population density, Q. What is population density? The Populati...

Q. What is population density? The Population density is the relation between the number of individuals of a population and the area or volume they occupy. For instance, in 200

Lipids - digestion process, Lipids are essential fats that have much import...

Lipids are essential fats that have much importance to the human body. Lipids are biological molecules that are insoluble in aqueous solutions and soluble in organic solvents are c

Describe the access in heart disease newborn, Describe the access in heart ...

Describe the access in heart disease Newborn? A secure peripheral or central intravenous access is very essential. Inotropic agents with vasoconstrictor properties like Dopamin

Explain the term lipids, Explain the term Lipids? If somebody discovers...

Explain the term Lipids? If somebody discovers that you have some knowledge of nutrition, you will be promptly shooted with two questions, first, "Oh! You really can tell how m

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd