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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are ions? What are the two types of molecules into which ions are classified? Ions are atoms or substances electrically charged by means of loss or gain of electrons.
Define the meaning of Vital signs Vital signs are measures of different physiological statistics, often taken by health professionals, in order to assess the most basic body f
Define Precautions for Estimation of Cholesterol Using Zlatkis Method 1. Use aldehyde free glacial acetic acid. 2. Use only glacial acetic for making up and adjusting the vo
Explain the metabolic pathways in the cornea. Metabolic Pathways: a. 88 per cent of glucose is utilized through glycolysis. b. Only 12 per cent of glucose utilized by
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Phosphate detergent as cause of water pollution
factors affecting nitrogen balance
Q. Explain process of Stress Testing in Women? Estrogen has been implicated as a cause of ST depression. For years it seemed that estrogen protect women from coronary artery di
SED A TIV E HYPNOTICS - They depress the activity of CNS. Reduce excitement, give feeling of calm. Higher doses induces sleep. Sleep inducing drugs are also called
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