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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Listeriosis Listeriosis is an important food-borne zoonosis caused by pathogenic species of Listeria. The disease affects both in man and animals. It is a serious invasive dise
Explain False Left Ventricular Aneurysm? False Left Ventricular Aneurysm : False aneurysm develops after acute rupture of an infarct. It is usually fatal, but a few survive
How do the plants classify according to their photoperiodism-based flowering? According to their photoperiodism-based the flowering plants classify as long-day plants, those th
This is the amount of blood that is returned to the heart by the veins. This is influenced by various factors. Inspiration decreases the pressure in the central veins and thus inc
As in several fields of molecular biology studies of E. coli have gives the model for subsequent investigations of transcription in eukaryotic cells. The basic mechanisms by that t
Q. What is Diverticular Disease? A common disorder of the large bowel, diverticulosis, is an early stage of the disease. It can be identified in 15% of the people over the age
scientific name of apple
Structure of human testis: The male reproductive system consists of a pair of testis. Testis are male reproductive organs and produce (male gametes) sperms and also secrete
the characteristics of flying fish belong to pisces
Physical map is the map of locations of the identifiable landmarks on DNA (for example restriction of the enzyme cutting sites, genes), regardless of their inheritance. Distance i
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