Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Toxoplasmosis, Toxoplasmosis Toxoplasmosis, one of the important parasitic...

Toxoplasmosis Toxoplasmosis, one of the important parasitic zoonoses, occurs all over the world, and is caused by a protozoan parasite Toxoplasma gondii. Its definitive hosts are

Are there living beings without cells, Q Are there living beings without ce...

Q Are there living beings without cells? Viruses are considered the only living beings that do not have cells. Viruses are constituted by genetic material RNA or DNA enwrapped

Regression of primitive streak, Regression of Primitive Streak As...

Regression of Primitive Streak As the inward migration of cells by the primitive streak ends progressively along its anterior to posterior regions the streak slowly regre

Extended point within pulp chamber-endodontics principles, Extended point w...

Extended point within pulp chamber: Gripping action: -    Steglitz forceps or pin pilers. -    Access cavity flooded with chloroform to dissolve the sealer. -    Grasp the

Foot-rot, Foot-rot Foot-rot is a term applied to the condition of feet ...

Foot-rot Foot-rot is a term applied to the condition of feet of cattle, sheep, goats and sometimes pigs. It is characterized by inflammation, necrosis and ulceration of underly

What is the endocrine function of the placenta, What is the endocrine funct...

What is the endocrine function of the placenta? The placenta is not a permanent gland of the endocrine system but it also has endocrinal function. The placenta produces estroge

Describe what types of results describe hypothesis, Why did giraffes develo...

Why did giraffes develop long necks? 1) Describe an experiment to test this hypothesis. Be explicit about the methods you will use, the setting, the time that the experiment wil

What is the call frequency, During residency: This alters from program to p...

During residency: This alters from program to program depending on the number of sites covered and number of residents. At McMaster, we do call roughly 1 in 7 or 8 (averages out to

Explain about urinary system, Explain about Urinary system Urinary sys...

Explain about Urinary system Urinary system provides us with the service of disposing the waste products carried by blood. This function is also known as excretion.

What raw materials require for the manufacture of margarine, What raw mater...

What raw materials require for the manufacture of margarine Soybean and peanut (or groundnut) oils are of great economic importance. Refined soybean oil contains branched fura

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd