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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
why brains can''t be transplant?
Variability refers to the differences in heritable traits exhibited by the individuals of a species. One of the major contributions of Darwin to the study of evolution was his book
Pathophysiology Large haemorrhages and fibrinous lesions vegetate along the inflaked edges of valves. The lesions develop on adjacent valve leaflets so that the edges adh
Explain the technology in which transgenic plant was constructed in which seeds that will produce plants for a single crop.
Adverse Effects The most common adverse effects of itraconazole are dose-related nausea and abdominal discomfort. Rash and serious hepatic toxicity can happen. The drug can ca
The genetically-modified (GM) brinjal in India has been developed for: 1. Insect-resistance 2. Enhancing shelf life 3. Enhancing mineral content 4. Drought-resistance
What are Taxonomic characters Taxonomic characters Classification is done on the basis of information we have. More information gives better classification. Modern taxonomy
THE CALORIE VALUE - The amount of heat liberated from complete combusion of 1gm food in a bomb caloriemeter (a closed metal chamber filled with O ) is its gross calorific
Describe what happens during activation of the lac operon. Lactose binds to the repressor protein, which causes the repressor protein to be released from the operator site. Th
Define about the Metabolism of Selenium compounds? Selenium compounds' are generally very efficiently absorbed by humans and selenium absorption does not appear to be' under ho
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