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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
A brief history of heart surgery : The dawn of Paediatric cardiac surgery was on August 26, 1938 when Robert, Gross, at the boston Children's Hospital, ligated ductus arteriosus
What are the main proteins that constitute the sarcomere? What is the function of those molecules in the muscle cells? In the sarcomere there are organized myosin and actin blo
Q. Medical and Nutritional Management for pectic ulcer? To provide physiological rest and support tissue healing, treatment should be based on providing rest to the affected ar
Q. Clinical manifestations of maple syrup urine disease? Clinical manifestations are expressed upon protein loading or with febrile illness. In most severely impaired enzyme de
Define Classification of proteins on the basis of attributes? Besides classifying proteins on the basis of soluble and insoluble, proteins have been further classified based on
Write functions of epithelial tissue
Protoplasmic Streaming and Tubular Peristaltic Flow Model The first of the above two models involves the well known phenomenon of cytoplasmic streaming in giant algal cells. C
Define Determinants of Food Security - Food Utilization? It is the proper biological use of food, requiring a diet providing sufficient energy and essential nutrients, potable
PSYCHOSOCIAL THERAPY: Psychosocial therapy is one of the important treatment modaIities used for the patients with mental disorders. It is given along with other therapies or
What is a food chain? The food chain is not branched it is linear sequence in which a living being serves as food for another, starting with the producers and going up to the d
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