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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Disadvantages of using yeast as a source of protein? 1. Less protein yield (45-60%) 2. Growth rate is low (1-3 h) 3. High nucleic acid content leading to the forma
Define the Binge Eating Disorder? Binge eating disorder is probably the most common eating disorder. Binge eating you may recall we studied as an element of bulimia nervosa. In
What is Interdental Threaded Cleaners The use of floss is recommended around any implant-supported bridge, crown, or bar. Instruct the patient to wrap the floss around the impl
what will happen to a plant kept in dark for 72 hours
Define General procedure of titration - Nutritional Biochemistry? In general a titration requires that we add precise amounts of the known titrant to the unknown analyte or ti
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Q. Symptoms of malabsorption syndrome? The most common symptoms are weakness, lassitude and marked weight loss. Steatorrhoea (excess fat in stools), anaemia and chronic ill h
Immunogenicity The safety and immunogenicity of the new vaccine was evaluated in two unpublished studies that are summarized in the package insert. Among 1280 adults (18-55 ye
Explain HIV/AIDS HIV/AIDS is another example of chronic infections. Its existence was discovered recently in 1981. The spread of HIV infection is widespread and can pro
Which of the below terms is used to describe animals which maintain a constant body temperature by producing heat by metabolic oxidations (muscle contractions) and losing excess he
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