Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Why are pteridophytes more common in humid places, Q. Why are pteridophytes...

Q. Why are pteridophytes more common in humid places? Pteridophytes are more common in humid places since they depend on water for their gametes to fecundate one another. In hu

Define physical capacity tests for aerobic capacity, Define Physical Capaci...

Define Physical Capacity Tests for Aerobic capacity? Aerobic capacity has already been defined. Aerobic capacity is usually measured using a tread mill test in which the intens

Define role of public nutritionist in health care delivery, Define the Role...

Define the Role of Public Nutritionist in Health Care Delivery? It is clearly evident from the foregoing discussion that nutrition is an important, though not the only, determi

Draw a section of the microscopic structure of human retina, Draw a section...

Draw a section of the microscopic structure of human retina and label any six parts in it. a)  Name the structure that determines the eye colour in humans. What is the normal f

What is the initial maintenance steps of implants, Initial Maintenance Step...

Initial Maintenance Steps of implants It is very essential, that from the beginning of the patient interaction regarding the implant therapy, through the diagnosis and planning

Nastic and epinastic responses, Nastic and Epinastic Responses Parato...

Nastic and Epinastic Responses Paratonic variation movements of plant parts in which direction of movement is independent of the direction of stimulus. Nastic responses are l

Theory or principle of determination of the quality of milk, Explain the Th...

Explain the Theory or Principle of Determination of the Quality of Milk Milk is an ideal medium for the survival and growth of microorganisms. It contain all the nutrients, i.e

Procedure for use microbiological laboratory equipment, Define Procedure fo...

Define Procedure for use Microbiological Laboratory Equipments? Now carry out this activity following the steps given herewith: 1. Observe each instrument (as mentioned abov

What is this atp synthesis called, How in the respiratory chain do electron...

How in the respiratory chain do electrons from FADH2 and NADH2 passing through cytochromes liberate energy for the ATP synthesis? What is this ATP synthesis called? FADH2 and N

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd