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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Factors Associated With Accident Cost in Administrative Functions Loss of prestige. Costs for notification, investigation and legal action. Loss of pu
Pathophysiology Asthma result from several physiological alterations, including altered immunologic response, increased airways resistance, increased lung-compliance, impaire
Carbohydrates Fifty five to seventy per cent of the required energy in animals is derived from carbohydrates. However, fats and proteins can also be broken down and used for
Define the Marasmic Kwashiorkor? In countries where the incidence of protein-calorie malnutrition (PCM) is high, a large number of cases show signs and symptoms of marasmus and
How much Vitamins should be taken for management of obesity? If adequate amount of fresh fruits and vegetables are included in the diet, the body stores of water soluble vitami
Recent molecular analyses indicate that the artiodactyls, which include hippos and camels, are paraphyletic, whereas cetaceans are monophyletic and cetaceans and hippos form a clad
Consumers Heterotrophs (other nourishing) are incapable of photosynthesis and depend on organic food derived from animals, plants or both. Consumers can be divided into two br
Explain The proteins stabilize emulsions The proteins stabilize emulsions and contribute physical and rheological properties, like thickness, viscosity, elasticity and r
Objective of the Radiographic Technique The ball bearings are opaque and their diameter have to be measured with the help of vernier caliper prior to use. (2 to 5 mm diameter b
African swine fever African swine fever (ASF), a highly contagious viral disease of swine and other porcine animals (generally confined to the African continent, part of Weste
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