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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
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What is the significance of Pharyngeal gill slit ? Lateral opening in the wall of the pharynx which allows water to enter into the mouth and exit through the pharynx. This is on
HOW CAN YOU JUSTIFY NEWTON THIRD LAW OF MOTION IN A TYPICAL PLANT CELL?
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DNA or RNA segment tagged with a radioactive molecule is called: 1. Vector 2. Probe 3. Clone 4. Plasmid Probe
Which of the following contributes to the degeneracy of the genetic code? A. The presence of 64 codons but only 20 amino acids. B. The ability of a single tRNA to bind to ma
What is the result of Post Myocardial Infarction Ventricular ? Results: Hospital mortality may be as high as 30 per cent. This depends on the extent of infarction and- pre-op
Q. What is the molecular formula of glucose? How can its structural formula be explained? The molecular formula of glucose is C 6 H 12 O 6 . Structurally glucose is a hexago
Cori Cycle Skeletal muscles have some mitochondria, yet are competent of vigorous activity. as a output, the little stores of oxygen are rapidly depleted, causing the muscle ti
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