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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Explain scombroid poisoning? Ingestion of improperly handled and stored seafood (yellow-fin tuna, skipjack, bonit and mackerel) causes scombroid poisoning. Maitoxin has been
Explain Preparation of burette for Estimation of Reducing Sugar by Fehling Soxhlet? Preparation of burette - Take a 50 ml burette. 1. Fix the burette into the burette holder
What is reproduction?
How many of each type of core histone would it take to wrap the entire human genome into nucleosomes? How has evolution solved the problem of producing such a large number of prote
Define the Drugs Effects on Excretion? Use of certain drugs can influence the excretion of certain substances. For example, besides their intended increase in sodium excretion,
peptide bond
Some species of plant are strongly adapted to pollination by certain insects. Characteristics which are regarded as adaptations to pollination by bees are: ( a) white o
Define features of Fusarium - Identification of Fungi and Yeasts? Identifying features of Fusarium: 1. Wooly, white fuzzy colonies changing colour to pink, purple or yellow.
How does the metachronal waves form in the ciliary row of protozoa ? Explain the cilia beating mechanism associates with it.
where is liquid tissue found
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