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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is Centrioles in cell structure? Most cells of higher organisms contain pairs of cylindrical bodies called centrioles, which help to organize microtubules in cell division
C a ttle and buffalo diseases Rinderpest: Rinderpest is the most destructive of the virus diseases of cloven-footed animals, viz. cattle, buffaloes, sheep, goats, pigs and
Dry Stigma - Category of Stigma The Cotton (Gossypium hirsutum) stigma is covered with long unicellular hairs. At the time of pollination, the stigmatic hairs show a distinct
How many carbon dioxide molecules are liberated after each cycle of the Krebs cycle? For a single glucose how many carbon dioxide molecules were already liberated by the aerobic re
Define Dietary Management of Sepsis with or without MODS? Before we begin with the dietary management of patients suffering from sepsis with or without MODS let us read a case
ROLE OF NaCl - It forms .9% of blood of mammal. It forms .7% of blood of frog. Bicarbonates of Na+ act as buffer for pH constancy. Involved in active transport of gluc
Q. How does the quantity of genetic material differ within the cell during the sequential phases of the cell cycle? The first period of the first phase interphase of the cell c
General Guidelines While working in the ward, you are always required to record. You should follow the general guidelines while recording which are as follows: All entri
Why do membranes figure so prominently in eukaryotic cells? What essential function do they serve?
Valuation of biological resources includes the process of deriving a monetary value to the things that are not sold in the market. Examples are the value given to fuelwood gathere
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