Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Physiology of the heart, The heart is the muscular pump that pushes blood t...

The heart is the muscular pump that pushes blood through the circulatory system. Each heartbeat can be felt as an arterial pulse. The heart valves when closed prevent the blood fro

Xylem transfer cells, Xylem transfer cells The lateral transport of io...

Xylem transfer cells The lateral transport of ions from root xylem to leaves probably takes place via xylem transfer cells which have two special features: The ce

Hemerythrins - respiratory pigments, Hemerythrins - Respiratory Pigments ...

Hemerythrins - Respiratory Pigments The Hemerythrins are rather rare. They take place in some animals belonging to the minor phyla like the sipunculid worms, some brachiopods,

Define endocytosis of glut4 transporters, Define endocytosis of GLUT4 Trans...

Define endocytosis of GLUT4 Transporters Person X is a healthy human who has volunteered to take experimental drug Y.  Person X has a normal dinner at 6 PM on April 1 and then

U, uses of conductometry in biological experiments

uses of conductometry in biological experiments

State the term in detail hydrostatic skeleton., State the term in detail hy...

State the term in detail hydrostatic skeleton. Formed from a fluid-filled and closed cavity surrounded by a body wall comprising muscles oriented in different directions. Muscu

How much fat contain vegetable butters, Vegetable Butters  Fats of thi...

Vegetable Butters  Fats of this group are derived from the seeds of various tropical trees and are distinguished by their narrow melting range, which is due mainly to the arra

What is autophagic intracellular digestion?, Autophagic intracellular diges...

Autophagic intracellular digestion is the cellular internal digestion of waste and residual materials. In general it is completed by lysosomes. Autophagic intracellular digestio

Illustrate about the insulin and insulin syring, Insulin, insulin syringe ...

Insulin, insulin syringe and insulin devices Insulin is the hormone used to treat diabetes mellitus. Insulin is given in the form of injection, because the gastro intestinal tr

Explain seed gums, Seed gums Polysaccharides derived from plants and se...

Seed gums Polysaccharides derived from plants and seeds have been in use for thousand of years. Seed gums are a group of polysaccharides with the following properties: S

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd