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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Class adverse effects of Protease inhibitors Many protease inhibitors can cause gastrointestinal distress, increased bleeding in hemophiliacs, hyperglycemia, insulin r
What is Waterston Shunt in palliative operations? In this, direct anastomosis between the posterior aspect of ascending aorta and anterior aspect right pulmonary artery is done
What is an example of a hypothesis which may explain why there is not a big representation of the class Reptilia found in polar regions? Beings of the class Reptilia are abunda
Define about skeletal muscle? A. The binding of calcium ion to a binding site on the troponin molecule leads to a movement of the tropomyosin molecule that, in turn, exposes a
Which kind of weak interaction can define higher order structure and in the same time DNA.
What is the logic of the transfusional compatibility concerning the Rh blood group system? The Rh + donor can only donate blood to the Rh + recipient. The person that lacks t
Define Gastrointestinal tract - Excretion of Zinc in Humans? Majority of zinc is lost from the body in faeces. Endogenous zinc in the form of enzymes or metallo-proteins is sec
Define Influence of Gardening on the Development of Botany? The influence of gardening on the development of botany may be seen in the work of John Gerard (1545-1612). Gerard p
Armamentarium 1. Denture duplicator or a modified plastic soapdish. (A plastic large soapdish with a base and cover can be adapted by drilling 2 mm holes interspersed and cover
Nutrition of Embryo Sac The morphology of the ovule suggests that the chalazal end is the main route for the entry of nutrients. The funicular vascular supply ends at the bas
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