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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
why we call leech as sangivorous
what is binomial nomenclature? What are the importance of it?
Determine the function of Osseointegration Osseointegration is also a measure of implant stability, which occurs at two stages- primary and secondary. Primary stability has be
Growth-form and Structure In a community the number of species and size of their populations vary greatly. A community may have one or several species. The environmental factor
The fact that some pure solutions of hydrocarbons do not readily evaporate at room temperature is a result of- Select one: a. London dispersion forces. b. The hydrophobic
Explain Class Bivalvia in animal kingdom? This name of this Class reflects the group's most distinguishing feature. Clams, oysters, scallops and mussels all have two shells tha
what we the strengths and weaknesses of pangenesis theory
In what way may we be able to take advantage of retro transposes in human gene therapy? How would this differ from our current use of retroviruses?
What is Molting? Explain in brief. The periodic shedding of outer body covering of an animal. The term is applied to a variety of things including: loss of the outer exoskeleto
I nfe c tio u s laryngotracheitis It is a slow, laterally spreading respiratory disease of chickens, pheasants, peafowl and turkeys caused by a herpes virus with high morb
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