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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Eye complications are very common in patients with diabetes. In fact, diabetes related eye diseases may not produce symptoms for a very long time and hence, it remains undetected t
Define Essentiality of Phosphorus for Growth of Micro-Organism Phosphorus is used in the form of phosphate salt by microorganisms. It is involved in formation of nucleic acids,
Pseudorabies Pseudorabies is a viral disease caused by pseudorabies virus (PRV) that is classified under family Herpesviridae. This disease is also known as “Aujeszky’s diseas
Electron Transport Inhibitors Several compounds, including specific drugs, chemicals and antibiotics have been known to inhibit the electron transfer reactions at specific
Determine about the Colloblast cell? The unique sticky discharge cells found in the tentacles of ctenophorans. They are used to capture prey, and although cnidoblasts resemble
A cell with a euploid number of chromosomes contains: A.one half of a complete set of chromosomes B.several complete sets of chromosomes C.the correct number of chromosomes D.two c
Fredrick Griffith published a series of experiments that collectively were dubbed "The Transforming Principle" in 1928. Which of the following prevented him from being able to decl
The nominalists deny the existence of Plato's "types". For them only individuals exist. Species are man-made artificial abstractions. But naturalists, the biologists who have actua
subject for botany assignment
One example of animals having a single opening to the outside that serves both as mouth as well as anus is: 1. Octopus 2. Asterias 3. Ascidia 4. Fasciola Ascidia i
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