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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
According to the Mendel's law the phenotypical characteristics would be determined by pair of factors (alleles) that separate independently in gametes. What are the major types of
how energy enters and then flows through a biologial system?
Q. Investigations process of constrictive pericarditis? Electrocardiogram Low voltage complexes can occur. Left atrial enlargement may be seen. Atrial fibrillation occu
Q. Explain about Metabolic diseases? Metabolic diseases, as you already know, refer to those disorders in which the various reactions in the cells are effected (production of e
Effects of long term malnutrition on children The effects of long time malnutrition are more severe in children. Unlike adults children do not have sufficient amounts of c
Explain Risk Factors For CAD? As has been noted, CAD is very common among the Indians and many studies have been undertaken to find out the reasons behind this predilection. Wh
The urea cycle which is also known as the ornithine cycle is a cycle of biochemical reactions occurring in several animals which produces urea ((NH2)2CO) from ammonia NH3. That cyc
Q. Concerning the maintenance of body temperature how do beings of the class Reptilia classify? Like amphibians and fishes, beings of the class Reptilia are heterothermic anima
Female Gametophyte The development of a female gametophyte is initiated with the enlargement of one of the megaspores (usually the one close to the chalaza in a linear tetrad)
Q. What are the main types of waste? The waste can be classified into many kinds, each of them carrying its own different environmental problem: recyclable waste, organic waste
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