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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Metabolic Significance of HMP Pathway Having gone through the HMP pathway, you would have got some idea about the significance of this alternative oxidative pathway for t
Isomerization of citrate Isomerization of citrate: In this step, citrate is isomerized to isocitrate by aconitase which has iron-sulphur centre as its prosthetic grou
questions and answers on human impacts on environment
In the typical Western diet approximately 80-140 G triglyceride and 0.5-1.5 G cholesterol are eaten daily. Following digestion, absorption and reesterification, triglyceride and c
general charactristics of phylum protozoa
Determine about the diseases Sleep attacks These are brief, often irresistible, episodes of sleep, probably slow wave, NREM, naplike sleep that last about 15 minutes and can oc
Accidents in Industry Workplace It is a hard fact to bear in mind that accidents in industry workplace are to be avoided or discarded and efforts in this direction has been in
Chromophore - Development of plant The chromophore is a tetrapyrrole molecule like chlorophyll, but unlike chlorophyll it is an open tetrapyrrole and contains no metal ion. It
Q. What is an example of positive feedback of the homeostatic regulation? In positive feedback the effect caused by an action stimulates the action even more. This is a rarer m
what is photosynthesis
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