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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the Functions of Folate? Folate, also known as folic acid, is essential for good health. Folate requiring reactions include those involved in phases of amino acid metabol
WHICH SOIL WILL BE FRUIFUL FOR GROWING LENTILS AND OTHER PULSES
Q. Illustrate the major cells of the nervous system? The major cells of the nervous system are the neurons. as well the neurons the nervous system is also constituted of glial
most recent classification of parasitic protozoa with its general characters
Define Principle for Estimation of cholesterol - Lipids? Zlatkis and associates have evolved a method of quantitative determination of cholesterol based on a reagent containing
What is an endospore? An endospore is a specialized, highly resistant, dormant structure formed within the vegetative cell of some bacteria e.g. Bacillus (rod), Clostridium (ro
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How do contraceptive pills generally work? Contraceptive pills generally have the hormones estrogen and progesterone. If taken daily from the 4th day after menses the abnormal
Q. Define Clostridium perfringens-associated gastroenteritis? Clostridium perfringens-associated gastroenteritis is a food borne disease which is frequently reported and is
In the experiments that led Fredrick Griffith to discover the "Transforming Principle", which of the following was the key step in illustrating that a substance within one cell cou
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