Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Respiration, explain the 4 stages of aerobic respiration

explain the 4 stages of aerobic respiration

Osteoporasis, OSTEOPORASIS - Reduction in bone tissue mass causing weak...

OSTEOPORASIS - Reduction in bone tissue mass causing weakness of skeletal strength. It leads to crush fracture. It occurs in pastmenopausal women & elderly man. Common

Distribution of stomata, Distribution of Stomata There are two paramet...

Distribution of Stomata There are two parameters normally used for expressing the distribution of stomata. Stomatal frequency: The number of stomata per unit area

Classification of coal by rank, Coal can be classified into following categ...

Coal can be classified into following categories in the order of rank. Peat:  peat is a brown and fibrous mass. It is the first stage of coalification. It is not used as

Excretory system, extensive note on the excretory system of amphibian

extensive note on the excretory system of amphibian

Learning objectives of gastrointestinal disease, Q. Learning objectives of ...

Q. Learning objectives of gastrointestinal disease? • explain the disease conditions, causes, complications of the disorders of the gastrointestinal tract, • discuss the ef

What are sensory receptors, Q. What are sensory receptors? Sensory rece...

Q. What are sensory receptors? Sensory receptors are structures specialized in the acquiring of information, mechanical pressure, like temperature, pH, luminosity and chemical

Cyanide - enzyme-activity control, Cyanide - Enzyme-activity Control P...

Cyanide - Enzyme-activity Control Plants generate cyanide from cyanogenic glucides and histidines. Ethylene biosynthesis is also accompanied by cyanide production. Nitrate red

Hutchinsons system of classification, Q. Hutchinsons System of Classificati...

Q. Hutchinsons System of Classification? John Hutchinson, a renowned British Botanist proposed 24 phyletic dicta or principles and based on these dicta he published a phylogene

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd