Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Chronic complications of diabetes, Q. Chronic complications of diabetes? ...

Q. Chronic complications of diabetes? These occur gradually when the diabetics do not monitor the blood sugar and they are careless about eating, exercise and the medications a

What percentage should be homozygous, If 40% gene pool is heterozygous then...

If 40% gene pool is heterozygous then what percentage should be homozygous?

Explain what is cyanosis and polycythemia, Explain what is Cyanosis and pol...

Explain what is Cyanosis and polycythemia? Cyanosis and Polycythemia: Central cyanosis involving the mucous membranes and trunk along with the lips and extremities in absence

What are possible treatments available, What are possible treatments availa...

What are possible treatments available for a herniated disc injury?

Animal pharming, A n i m a l pharming Using animals...

A n i m a l pharming Using animals as bioreactors is also cost-effective and advantageous because animals naturally carry the cellular mechanisms needed to

Atoms, the mass number is used to calculate the number of _________in one a...

the mass number is used to calculate the number of _________in one atom of an elemement. In order to calculate the number of neutrons you must subtract the _______from the ______.

System that permits movement and fixation to echinoderms, What is the syste...

What is the system that permits movement and fixation to echinoderms? The system that allows movement and fixation to substrates in echinoderms is known as the ambulacral syste

Writing transport and flow expressions in simbiology, How do I write the ex...

How do I write the expression for reaction rate (transport) of species between the arterial compartment and the other tissue compartments in symbiology?

Name two forms of respiration gives more energ, Which of the two forms of ...

Which of the two forms of respiration (aerobic and anaerobic) gives more energy from a given quantity of food? Aerobic respiration gives more energy than anaerobic respiration

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd