Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Explain scombroid poisoning, Q. Explain scombroid poisoning? Ingestion ...

Q. Explain scombroid poisoning? Ingestion of improperly handled and stored seafood (yellow-fin tuna, skipjack, bonit and mackerel) causes scombroid poisoning. Maitoxin has been

Preparation of burette for estimation of reducing sugar, Explain Preparatio...

Explain Preparation of burette for Estimation of Reducing Sugar by Fehling Soxhlet? Preparation of burette - Take a 50 ml burette. 1. Fix the burette into the burette holder

How many of each type of core histone, How many of each type of core histon...

How many of each type of core histone would it take to wrap the entire human genome into nucleosomes? How has evolution solved the problem of producing such a large number of prote

Define the drugs effects on excretion, Define the Drugs Effects on Excretio...

Define the Drugs Effects on Excretion? Use of certain drugs can influence the excretion of certain substances. For example, besides their intended increase in sodium excretion,

Some species of plant are strongly adapted to pollination, Some species of ...

Some species of plant are strongly adapted to pollination by certain insects.   Characteristics which are regarded as adaptations to pollination by bees are: ( a) white o

Define features of fusarium - fungi and yeast, Define features of Fusarium ...

Define features of Fusarium - Identification of Fungi and Yeasts? Identifying features of Fusarium: 1. Wooly, white fuzzy colonies changing colour to pink, purple or yellow.

Zoology of Protozoa, How does the metachronal waves form in the ciliary row...

How does the metachronal waves form in the ciliary row of protozoa ? Explain the cilia beating mechanism associates with it.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd