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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What makes the cluster of atoms of carboxylic acid functional group an acid? a) a non-polar O-H bond near another electronegative oxygen. b) an ionic O-H bond near another el
A genomic DNA library is made from the genomic DNA of an organism. For instance, a mouse genomic library could be made by digesting mouse nuclear DNA with a restriction nuclease to
Coo k in g loss and quality Yields and cooking losses of products are important as these determine acceptability and economics of products. Higher cooking losses increase th
In a population of 200, 90 are homozygous dominant, 40 are heterozygotes, and 70 are homozygous recessive for a particular trait. What do you expect the frequencies of the dominant
Transferring the Patient form ICU to Ward The patient is made to ambulate in ICU before shifting to ward . vitals are stabilized. The ward nurse is explained the pa
Define the Bioavailability of cyanocobalamin? Vitamin B 12 is widely available. Availability is more from non-vegetarian foods as described earlier under the food sources sec
Define Measuring Direct Aerobic Fitness (VO 2 max) For direct measurement of VO, max, the subject is asked to perform exercise for 12 to 15 minutes. A stationary bicycle or a
Heat Loss Temperature regulation is extremely uneconomical if it depends only on variations in metabolism. Therefore, mechanisms for losing excess heat have been developed by
Why are transgenics considered a threat to the environmental safety? Transgenics can be dangerous to the entire biosphere as the transfer of genes among species may have immed
Q. Is all the cartilage in the body the same? There are 3 types of cartilage in the human body: 1) hyaline cartilage; 2) elastic cartilage and 3) fibrocartilage. Hyaline cartil
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