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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Rumen protection of nutrient (bypass nutrients) technology The amino acid and energy requirements of medium and high yielding cows and buffaloes are not fully met from the micr
What is lactic acid
Elaborate Evolution by Natural Selection ? The conelaboratecept of natural selection lies at the heart of Darwin's Theory of Evolution. Darwin understood that plants and animal
what is glycolysis?
Suppose you were planning to use liposomes in an attempt to deliver drugs to a particular type of cell in the body, for example, a fat or muscle cell. Is there any way you might be
CILIA AND FLAGELLA Purkinj e first observed ciliary movement. Hodge studied detail structure of sperm flagellum. Manton studied structure of cilia in plants.
Define the Insulin resistance - Obesity? Insulin resistance is a condition in which your body cells cannot utilize insulin efficiently although sufficient amounts are secreted
Types of Surgery : Tricuspid valvotomy is done as an open procedure at the end of surgery on mitral and/or aortic valve. It can be done on an arrested heart or on a beating heart
Healthy human female X is 25 years old and not pregnant. During the postovulatory phase of X's menstrual cycle, A. there are high blood plasma levels of LH and FSH.
Classification of zoonoses More than 300 zoonoses of diverse etiologies are recognized. Thus, a very large number of zoonoses call for classification, especially for teaching
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