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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Name the disorder in which the immune mechanism of the body of a patient gets suppressed. Which pathogen is responsible for it? Give any two reasons of its transmission.
Stem Cell Stem cell therapy could enable individuals to survive and contribute their alleles to the gene pool who might not have already done so thus increasing genetic diversi
Describe about Postural Changes in Dynamic Auscultation? a) On sudden assumption of supine position from standing on sitting position or sudden passive elevation of both legs:
Aortic Stenosis Aortic stenosis is due to an obstructive lesion that interferes with the blood flow out of the left ventricle. Depending upon the site of obstruction to the o
Define Efficiency - terminologies used in chromatography? Efficiency in chromatography is expressed as number of theoretical plates. H = L/N Where, H is heigh
Q. Explain Right and left Dominant Circulation? Right Dominant Circulation: In 85 per cent of patients, the right coronary artery goes on to form the AV nodal artery, the poste
Explain An Overview of Water Soluble Vitamins? Vitamins, we already know, are classified by the materials in which they will dissolve. Fat-soluble vitamins -vitamin A, D, E a
Q. Medical therapy for tricuspid stenosis? Medical therapy is ineffective. With diuretics, symptoms of congestion are replaced by that of low cardiac output state. Occasional c
What is meant by Gene Expression? The synthesis of protein under the influence of gene is called Gene Expression. All human cells are derived from a single cell, fertilized egg
What are the organs that are part of the female reproductive system? The organs that comprise the female reproductive system are the ovaries, the Fallopian tubes (or uterine t
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