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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
The pancreas performs digestive and hormonal functions. The enzymes secreted by the exocrine tissue in the pancreas help in the break down of carbohydrates, fats, proteins, and
Explain about the Hypokalemia? Normal serum K ranges from 3.5-5 mM/L. Hypokalemia or low plasma K levels can occur with a net shift of K from the plasma to the cells. This shi
Creb binding protein (CBP) is a histone acetyl transferase (HAT) protein. What role does the addition of acetyl groups to histones play during development? A. The addition of
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How can an organism that once underwent contact with an antigen be immunized against future infections by the same agent? This phenomenon is known as immune memory . When an a
information on climbing beans
character and classification of pigeon
How to Calculate the Biological Value of Protein? A method for determining the biological value of proteins was developed by Mitchell in 1925. It measures the quantity of dieta
Pollen Tube Growth There are significant differences in the fine structure of the tips of pollen tubes in compatible and incompatible pistils such as Lilium. Tubes growing in
Ethylene - Apical Dominance Ethylene also plays a role in the inhibition of lateral bud growth. It is present in those tissues like shoot apex where auxins are also present. I
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