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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Effects of long term malnutrition The effects of long term malnutrition are more severe in children. Children do not have sufficient amounts of carbohydrates and fats in
Q. Explain Spoilage by fungi and bacteria? The dominant spoilage organisms in fruits are fungi, as their pH is low (normally 5),whereas the pH of vegetables is fiom 5.0 to 7
What is factor VIII? What is the genetic disease in which this factor is absent? Factor VIII has the function of activating factor X that in its turn is essential for the trans
i need to decribe, analyse and evaluate the 2cells
is crossing over of chromosomes is a method?
How will the urine concentration be affected if the henle''s loop of a person is removed and his PCT is joined directly with DCT?
If levels of ATP become high in a cell (i.e more than the cell needs at that time), describe the mechanism that the cell would use to alleviate this situation (include the name of
Describe Fourth Heart Sound in details? S 4 is generated during the atrial-filling phase. It is a low frequency event. LVS, is best heard over apex. Mechanism of S 4 generat
Overturn - Thermal stratification The summer or winter stratification is seasonal. Circulation of lake water occurs twice a year, in the spring and autumn (fall) seasons by a
Q. How can an organism that once underwent contact with an antigen be immunized against future infections by the same agent? This phenomenon is called as immune memory when an
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