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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is the major cell organelle involved in cell digestion and what are the properties of that organelle that enable it to do the task? The organelles accountable for intra
Theory of Germplasm (i) 'August Weismann' (1889) suggested the theory of continuity of germplasm. (ii) He referred to the reproductive cells as germplasm and
At the starting of the first round of elongation, the initiation codon (AUG) is positioned in the P site with fMet-tRNAfMet bound to it by codon-anticodon base pairing. In The nex
Define the term- Adaptation The terms scotopic and photopic vision are relative to the lighting conditions of an individual place. Light adaptation is a very quick process and
Ask question #Minimum 100 words accepted Which gas is essential for pjoto synthesis
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State in brief about the soil horizon A soil horizon may be defined as "a horizontal layer of regolith, approximately parallel to the soil surface, and possessing relatively
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Define Physiologic Functions of Iodine? Thyroid hormone performs multiple functions as regulator of cell activity and growth. The hormone has crucial metabolic roles in the foe
Explain Stavudine It can be given either in initial combination therapy or after failure of regimens containing other NRTIs. Cross-resistance with zidovudine is frequent, ho
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