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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Carbohydrate characteristic chemical features include: Select one: a. the potential to form multiple hydrogen bonds. b. the existence of one or more asymmetric centers.
What is the cardiovascular system The cardiovascular system, nervous system, kidneys and special sensory organ, eyes is directly affected by diabetes and leads to fatal conditi
Uncompetitive inhibition In this type of inhibition, the inhibitor only binds with the enzyme-substrate complex making it inactive. As a result, the product formation
Which are the brain regions associated with memory? According to researchers some of the main regions of the nervous system associated with the memory phenomenon are the hippoc
Which of the following statements is true regarding a trihybrid cross between two true-breeding homozygous individuals with contrasting phenotypes? Which of the following statement
Determine about the Chloroplast One of the most distinguishing features of all eukaryotic plant cells is that in addition to mitochondria, they contain special light harvesting
Life cycle of Parasite - Protozoan The life cycle of the parasite in man begins when an infected mosquito, while biting and taking a blood meal, injects sporozoites into the
Define Clinical Trials for Changing nutrient requirements with age? Several clinical trials provide evidence of changing nutrient requirements with age. The metabolic demand fo
Q. Discovery of insulin? The discovery of insulin has dramatically changed the lives of people having type 1 diabetes. With this wonder drug diabetics can lead a normal, enjoya
Explain Procedure for Capsule Staining in a Culture? We have learnt that Capsule staining can be undertaken either by negative staining or Anthony method. Now carry out the exe
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