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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is PCR? How does PCR works? The PCR, polymerase chain reaction, is a method to synthesize many copies of specific regions of a DNA molecule known as target-regions. Its in
Q. Generally how does a male animal realize that the female is receptive to copulation? In most vertebrate species with internal fecundation the females have reproductive cycle
Many disease states are heralded by changes in the blood count Leukocytosis can be a sign of infection. Thrombocytopenia can result from drug toxicity. Pancytopenia is ge
Glucose-6-phosphate to Glucose Glucose-6-phosphate to Glucose : Glucose-6-phosphate is converted to glucose by glucose-6-phosphatase which is present in intestine, liver and
Endosperm with micropylar Haustorium A very prominent and aggressive micropylar haustorium is seen in Impatiens. Here the division of the primary endosperm nucleus is followed
Outline three client considerations when taking an accurate pulse oximetry reading.
KIDNE Y (RENAL FAILURE) - RF - Partial or total inability of kidney to carry excretory functions is called RF. It leads to uremia (excess of urea in blood), salt-water imba
Genome wide association studies must account for the fact that covering the entire genome with marker loci will produce ______ associations between linked marker alleles and possib
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N e c r otic enteritis It can be designated as enterotoxaemia of chickens, turkeys and ducks caused by Clostridium perfringens. The toxin released by this spore-bearing a
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