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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
An impermeable membrane separates one liter of a 0.01 M glucose solution in water in the left compartment from one liter of a 0.1 M glucose solution in water in the right compartme
Advances in neurodiagnostic techniques Major scientific and technology advances have made it easier to correlate behaviours suspected as having a neurological basis with actual
Explain the Bunsen Burner? It is a type of gas burner that gives very hot flame by allowing air to enter at the base and mix with the gas. It is used - (a) For sterilizing i
Diuretic Therapy One of the aims of treatment of congestive heart failure is directed toward controlling salt and water retention (central or peripheral edema). Diuretics provid
A cross among wild-type fruitfly Drosophila melanogaster [normal bristles and grey body (s+s, e+ e)] with a mutant fly [short bristles and ebony body (ss ee)] produces following p
Subphylum Opalinata Body covered with longitudinal rows of cilium like ,organelles but true ciliature absent. Parasitic; cytosome lacking; two to many nuclei of one type.
What is Lactate Lactate is transported to the liver in the Cori cycle (lactic acid cycle) and is converted to pyruvate. Hepatic gluconeogenesis then converts lactate ba
Many disease states are heralded by changes in the blood count Leukocytosis can be a sign of infection. Thrombocytopenia can result from drug toxicity. Pancytopenia is ge
CHOLINE Found in egg liver & kidney. Synthesized in body with acetyle coA. It forms acetylcholine. Deficiency causes damage to kidney.
Inhalation Route for Injection Therapies that can be administered by inhalation are oxygen, humidification-cool mist and steam, administering local medication. The commonly u
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