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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Requirements of Glutamine in diverticular disease? Glutamine: Wile specific nutrients that may have an impact on diverticula disease have not been studied as thoroughly as t
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Describe the meaning of Electrical Stimulation Brain stimulation has been used to map connections in the brain and to elicit changes in behaviour. Much of the pioneering work o
Q. Antimicrobial Barriers - proliferation of microorganisms? The first barrier is the integument: a physical barrier to protect the food, e.g., the shell on eggs, the skin on
General Principles for Incisions The general principles for placing incisions so as to create surgical flaps remain the same regardless of the reason for creating them. These a
Q. If a person eats raw or badly cooked meat infected by Taenia solium or Taenia saginata will this person develop taeniasis or cysticercosis? If a person eats badly cooked or
Standardisation Research of Luria Nebraska There are published manuals for the Luria Nebraska that define the battery in detail and provide information pertinent to validity,
Indications for Surgery : 95 per cent of ventricular aneurysms occur after transmural myocardial infarction. Trauma, Chaga's disease, sarcoidosis and congenital causes are the ot
Induction of Defensive Barriers in Plants Several polymers deposited near surface of plants form an effective barrier against invasion or stress. Cuticle (which consists of a
In mammalian body, this element plays numerous important roles. Try to identify this element with the fewest number of clues. This element is needed to insure the integrity and per
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