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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Radial artery: Carpentier used radial aartc14y us a conduit for CABG in 197 1. He had 30 percent early occlusion of the graft and it was given up. The mail1 problem was radi
Factors Affecting Fertilizer Requirements The rapid increase in the use of fertilizers is based on the fact that large profits are obtained from the money invested in them. The
Bacterial food poisoning Food poisoning is an acute gastro-enteritis or intestinal disorder resulting from the ingestion of foods contaminated with either living microorganism
How is the gastric mucosa protected from the acid pH of the stomach? The gastric epithelium is mucus secretory, i.e., it makes mucus. The mucus covers the stomach wall preventi
SYM P A THETI C NERVOUS SYSTEM These sympathetic chain are connected with ramus comminicause of thoracic & lumber part. Due to this reason this system is called as t
Iron Iron is an essential component of the proteins involved in oxygen transport or utilization. These proteins, including hemoglobin, myoglobin and a number of cytochrome and
Telenursing Telenursing refers to the exchange of expertise in various areas like nursing diagnosis data over a distance. It is divided into the following categories: a)
How are amino acids brought to the cellular site where translation takes place? What is an anticodon? Amino acids are brought to ribosomes by RNA molecules called as transfer R
Describe the Frequency of low-risk coronary patients? For many low-risk coronary patients, particularly those following myocardial revascularization procedures, rehabilitation
Primitive Nervous System - Nerve Net A nervous system creates its appearance for the first time in the phylogeny among Cnidaria. In this group the nerve cells make an irregula
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