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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
what are the chemical changes takes place during cleavage
After stinging its victim, the honeybee leaves behind the barbed stinger, poison sac, and muscles that continue to pump venom into the wound. A study compared two methods of removi
Leishmanias – Flagellates Species of the genus Leishmania are parasites of mammals including man. Leishmanias infections occur over wide regions of the world from Asia, the ne
Explain the barrier function in the cornea. Barrier Function: It is the first line of barrier between the external environment and the corneal stroma. The superficial epi
Mr. Jones I.V. was hung at noon. It is a liter bag and is infusing at 65 ml/hr. How many hours will this bag last?
Gametogenesis - Human Development Gametogenesis as you are responsive is the process of formation and development of specialized reproductive cells, ova in females and sperms
Define the effect of Vitamins (A, D, K and B-Complex) on athletes? Vitamins A, D and K have been found to have no ergogenic effects. Ingesting large doses of these vitamins hav
Fertilisation: Fertilisation is the process of fusion of male and female gametes. For fusion of male and female gametes, pollen grains have to reach the surface of the stigm
Reaction - Processes in Succession This is the most important stage in succession. The mechanism of modification of environment, through the influence of living organisms on i
Porifera - Regeneration in Invertebrates Sponges have immense regenerating ability and show two ways of doing so as displayed below. : (a) Regeneration from small segments-
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