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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Wet Stigma - Category of Stigma Petunia shows several randomly distributed 2-celled papillae on its surface. In a developing stigma, the epidermis is covered by a continuous,
Explain Ledge bypass - Non-surgical Endodontic Retreatment Ledge bypass - StSt not NiT cause it has shape memory Ledges from outside the curvatures. The file t
Flow cytometry is an analysis of biological material by detection of light- absorbing or fluorescing properties of cells or subcellular fractions (that is chromosomes) passing in
Figure shows part of a DNA molecule (a) (i) What do the dotted lines in Figure represent? (ii) Name J to M. J.............................. K..............................
What are the three phases into which the HIV infection is often divided? The HIV infection is often separated into three phases: the acute phase, from the infection to 1 until
what is biology
Explain the Rotary Dryers? Rotary dryers are widely used to dry relatively large throughputs of granular products and by products in a number of industries, including the food
Puerperal Psychosis: Child birth can precipitate schizophrenia, depression and mania. Management: it depends on the nature of the symptoms. Danger to self and othcrs (inc
What is the control center of the cell? The nucleus contains DNA, which having the instructions for all the functions of the body.
Describe the difference between dna and protein. (structural unit, linkage bond, primary and spatial structure)
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