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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Problem 1: "Structural Genomics aims at determination of the 3D structure of all proteins". Describe with techniques Show genomic techniques for determination of 3D struc
An increase in the calcium conductance of all sarcoplasmic reticulum membranes of a skeletal muscle with no external forces on it leads to A. Increased binding of calcium ions
What are abiotic factors? The Abiotic factors are the nonliving elements that constitute a given environment, like light, temperature, water, minerals, gases, atmospheric press
In the previous sub-section we discussed the possible ways by which variabiliti can, be generated. We shall now examine one instance that illustrates the consequence of variability
Q. Explain Ciguatera Fish Poisoning? Ciguatera probably represents the most common cause of poisoning from fish in tropics. Ciguatera comprises over 50% of all reported case
Define Diarrhoea problem of infants & preschoolers nutrition? We have just covered control and strategies in diarrhoea management in the above section. Crawling, unclean hands,
Explain Water - An Essential but Overlooked Nutrient? You may already know that the total body water (TBW) constitutes 50-60% of the body weight. A 70 kg 'standard male' contai
Q. Explain procedure of Aortic Valvuloplasty? BAV is usually performed by the retrograde transarterial approach. Often another catheter is placed in the LV through transseptal
Describe Infectious Agents ? Recently there has been increasing interest in the relation between chronic low grade systemic inflammation and mortality of coronary artery diseas
Gastrointestinal Antibiotic prophylaxis is recommended for esophageal surgery in the presence of obstruction, which increases the risk of infection. After gas troduodenal surge
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