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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
State in brief about the Macronutrients Each element is specific in its function in plant metabolism, however, the exact functions for a number of them are still not known. T
Describe the location and structure of the pituitary gland and explain its structural and functional relationships with the hypothalamus.
In the evolution of whales, there is evidence that whales and hippo form a monophyletic group. Show all the evidence that supports the close relationship between whales and hippos
BIOLOGY - A SCIENCE OF EXCEPTION - Some common examples to prove it are - 1. Embryos of dicot plants have 2 cotyledons but in cuscuta (Dodder plant or amer bel or Aka
Where does cartilage occur in a joint and what it its function? Cartilage might be found covering the surface of bones where they meet in a movable joint. Cartilage decreases f
T hese are some important uses of biodiversity. 1. Source of food, medicines and drugs: (a) Wheat, rice and maize are three carbohydrate rich crops and contribute
Define Potential Health benefits from resistant starch? Like dietary fibre, RS can also play a potential role in helping to maintain or improve health of an individual. As you
Mice (Mus musculus) have 40 chromosomes per diploid cell (2n=40). How many double- stranded DNA molecules, and how many chromosomes are there in a mouse cell that is in the G2 stag
Q. What is the difference between sexual gametes and spores? Do humans present sexual gametes or spores? Sexual spores are structures generated from meiosis with ploidy number
Explain the Objectives of Taxonomy Let us summarise the objectives of taxonomy: 1. To inventory the world flora and fauna 2. To provide a method for identification and co
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