Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Define about the gastric cancer, Define about the Gastric Cancer? Surgi...

Define about the Gastric Cancer? Surgical treatment is a very common mode of treatment in gastric cancer. Radiation and/or chemotherapy are given for patients with resected but

Predict whether narrowing or widening of the blood vessels, Body temperatur...

Body temperature is homeostatic ally regulated around a set point. Given your knowledge of negative feedback and homeostatic control system, predict whether narrowing or widening o

What is the menstrual cycle, What is the menstrual cycle? The menstrual...

What is the menstrual cycle? The menstrual cycle is the periodic succession of interactions among hormones and the organs of the female reproductive system that, after the star

Fusion of egg and sperm pro-nuclei, Fusion of Egg and Sperm Pro-Nuclei ...

Fusion of Egg and Sperm Pro-Nuclei The fusion of the sperm and egg which is the beginning of the fertilization process is completed with the fusion of the male and female pro

Types of parthenogenesis, TYPES OF PARTHENOGENESIS - 1 .      NATURAL...

TYPES OF PARTHENOGENESIS - 1 .      NATURAL PARTHENOGENESIS - In many animals natural parthenogenesis is common process & is a method of reproduction. It is of two typ

How does iodine kill germs, How does iodine kill germs? The microbiocid...

How does iodine kill germs? The microbiocidal action of Iodine is because of the active form, I2, which is polarized by water and like all halogens (chlorine, fluorine, bromine

Types of parthenocarpy, Types of Parthenocarpy Three types of Partheno...

Types of Parthenocarpy Three types of Parthenocarpy are generally recognized: Genetical Environmental, Chemically-induced. Genetical Parthe

Define natural disasters - public nutrition, Define Natural Disasters - Pub...

Define Natural Disasters - Public Nutrition? Disaster prone settings also shape the intensity and prevalence of food insecurity, these consist of poor who are exposed to recurr

Name the reserve food materials in pheophyceae, Name the reserve food mater...

Name the reserve food materials in pheophyceae Ans) Reserve food materials in pheophyceae are laminarin and mannitol

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd