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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Which of the following is true for the ATP-sensitive potassium channel? A. The channel is a spanning protein with a receptor site for ATP located on an extracellular region of
Define B-Complex Vitamins required for elderly? B-Complex Vitamins: Various B complex vitamins especially B folic acid, B 6 , and riboflavin are found to affect favourably t
Transplantation - Clonal Propagation The shoots or plantlets multiplied on a medium containing organic nutrients, show poor photosynthetic capability. Moreover, in these pla
what is role of macro and micro nutrients
Q. Show the Impressions at first - Transitional Restorations? The dental technique will have modified the abutment to the desired shape and will now be able to fabricate the tr
Eukaryote is the type of cell found in several organisms including single-celled protists and multicellular fungi, plants, and animals as well; characterized by a membrane-bounded
What is the meaning of Oedema? In some pathological conditions the body is in a positive water balance; that is the intake of fluids is greater than the excretion, and the pati
Q. Association of Fibrinogen With Cardiovascular Risk Factors? Ans. Fibrinogen, a large glycoprotein made mostly in the liver, is a clotting factor that activates thrombin
Bud grafting A 'T' shaped incision is made on the bark of the stock. A bark is removed on either side of vertical cut. A single bud scion with a little wood is placed
Define Functions of Calcium in Human Body? Calcium salts provide rigidity to the skeleton and calcium ions play a role in many if not most, metabolic processes. In the vertebra
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