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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Procedure for Performing the Completed Coliform Test? Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise f
Q. Introduction of Quality Attributes of Food? Seasonal fruits and vegetables are grown in plenty, and are available in their respective seasons in abundance. While choosing th
Q. What are the six criteria used to build a complete evolutionary branch of vertebrates? Dichotomy in each of the six following criteria builds the vertebrate evolutionary bra
Q. Vitamins requirement during myocardial infarction? The requirement of vitamins and minerals is largely governed by the existing nutritional status and the clinical parameter
OBSESSIVE COMPULSIVE DISORDER (OCD): OCD is another common neurotic disorder seen in the hospital. Definition Obsessive Compulsive Disorder is the disorder characte
Explain Enzymatic Proteins The most varied and most highly specialized proteins are those with catalytic activity - the enzymes. Virtually all the chemical reactions of organic
What is eukaryotic cell?
adaptation of the phylum protozoa to its function
Define the Prevention of Adverse Food Reactions? Considering the increasing incidence, cost and morbidity associated with allergic reactions, it is perhaps useful to design pre
Chromosome walking is the technique for cloning everything in genome around the known piece of DNA (the begning probe). You screen the genomic library for all the clones hybridizi
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