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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
State in detail about the thylakoid membrane The thylakoid membrane contains all the factors associated with the photosynthetic electron transport leading to the generation of
Advantages of ecological pyramids?
Gum Karaya Gum karaya (sterculia gum) is the dried gummy exudate from Sterculia urens Roxburgh and other species of Sterculia (Family: Sterculiaceae) or from Cochlosperm
Q. What is the relationship between environmental resistance and the population growth according to the biotic potential curve and the real population growth curve? The differe
What is the logic of the transfusional compatibility concerning the ABO blood group system? The transfusional compatibility for the ABO system takes into account the antigens s
Q. What is the full chemical equation of photosynthesis? The full chemical equation of photosynthesis is given below: 6 CO 2 + 12 H 2 O + light --> C 6 H 12 O 6 + 6 H 2
What are the specialized structures that help the adhesion between cells? The structures responsible for the union of the epithelial cells are known as cell junctions. The main
These mycotoxins are structurally and biologically related to the aflatoxins, and like the latter, they cause hepatocarcinogenic activity in animals. Among the organisms that prod
Skull of Pisces?
Give a brief overview of "HIV & Streptococcus Pyogenes" including signs and symptoms. Who is typically affected by this infection?(Children? Immunocompromised patients?)? What is t
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