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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How can I construct a fat molecule whose fatty acid tails are 4 carbon atoms long
Working of Specific Acquired Immunity Primary lymphoid organs, as you already know, are thymus and bone marrow. Secondary organs are lymph nodes, spleen and lymphoid tissue. Wh
Ecosystem Degradation and Wildlife Man is an animal with ecological requirements just like any other animal. It constantly needs a source of energy and.minera1 nutrients for i
Determine the steps in surgical therapy a) The first step in surgical therapy is to reflect the tissue and degranulate the defect. b) It is followed by exposing and treating
How did Pasteur's experiment answer the objections raised by supporters of the "vital force" hypothesis? Pasteur's experiment allowed air from the out- side to mix with air fro
Define the term - Stimulus Stimulus and response characteristics of the tests themselves, as well as of the test instructions, become exceedingly important considerations. In g
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what is a bio molecule
Explain Heat Preservation - Method of Food Preservation? The process of heating was used centuries before its action was understood. Food is heated up or cooked. Heat kills mi
Q. What is the relation between the hypophysis and the hypothalamus? The hypothalamus is a part of the brain situated just above the hypophysis and the hypothalamus gets centra
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