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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Are the extraembryonic membranes the same in all vertebrates? The presence of each extraembryonic membrane varies according to the vertebrate class. In fishes and amphibians
MALE REPRODUCTIVE DISORDERS Generally, about 5-10% of cattle bulls reaching sexual maturity would be suffering from poor reproductive efficiency or sterility. Crossbreeding ha
Branemark's Theory of Osseointegration In 1952, Swedish orthopedic surgeon, Branemark, conducted microscopic studies done in situ of the bone marrow in rabbit's fibula with in
Describe a. the factors responsible for promoting lactation, and b. the mechanism responsible for the cessation of lactation when a mother weans her baby.
Determine Nutritional Requirements for High Altitude? High terrestrial altitudes and mountains have aroused great fascination and cham for mankind. Every year millions of peopl
The excretory organs and excretory materials of plants and animals
Define briefly about the Seed Proteins? Although a large number of plants produce seeds having protein contents in excess of 15%, only a few are utilized for food, eg. soybean,
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What are the forces that facilitate to make water to flow within the xylem from the roots to the leaves? Water enters the roots because of the root pressure and a water column
What are the uses of microorganisms
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