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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What are carbohydrates? Carbohydrates are important organic compounds widely distributed in animals and plants. Plants can synthesize carbohydrates by the process of photos
Marine Ecosystems Marine ecosystems cover 70 per cent of the earth's surface and have an average depth of 3.750 m (the greatest known depth being 10,750 metres in the Mariana
Amino acid is any of a class of 20 molecules which are combined to form the proteins in living things. Consisting of the general formula NH2-CHR-COOH, where "R" is the side chain
List three important discoveries that resulted from the Human Genome Project. Answers should contain three of the following: Only about 2 percent of the human genome encodes p
Explain the Steps for Injecting Insulin Injection It is important to teach patient to take injections at regular times, 30 to 60 minutes before meals. Take insulin everyday, ev
Signal transduction occurs when an extracellular signaling molecule activates a cell surface receptor. In words, this receptor change intracellular molecules changes a response. Th
Adenine is one of the four nitrogen-containing bases occurring in the nucleotides, the building blocks of organic macromolecule group called as nucleic acids (DNA and RNA). Adenin
HOMOPO L YSACCHARIDE S (= HOMOGLYCANS) These are complex carbohydrats that are formed by repeated condensation or polymerisation of only one type of monosaccharide monome
Which of the following processes in capillaries in the lung assist in the removal of carbon dioxide from the body? A. Net flux of carbon dioxide from red blood cells into plasm
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