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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Complications in celiac disease? Patients with severe form of celiac's disease for long period are at risk for several complications mainly due to nutrient absorption probl
Diabetes Mellitus Management after Open Heart Surgery : Diabetic patients undergoing coronary artery bypass surgery who are on oral hypoglycemic drugs are pre operatively s
Concerning reproduction what is the function of the testicles? The testicles are the male gonads, i.e., the organs where the production of gametes takes place. In human beings
Q. Extractive use of biodiversity? This includes direct use such as harvesting of wild plant species for use as food, fuel, fodder, fibre, shelter or medicine and hunting anima
An episode of diarrhoea can be acute (recent origin) or chronic (extended duration and repeated episodes) in nature. You may recall reading in the Food Microbiology ans Safety Cour
Name some emulsifiers which are present in nature? There are many emulsifiers which are present in nature such as phospholipids e.g. lectin which is present in egg yolk which a
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Explain the dispersed phase of colloidal system Colloidal systems are not restricted to the dispersion of a solid in liquid. Each of the three states of matter - gaseous, soli
Delimitation and Limitation: There may be many aspects of the problem that need to be explored, but it is difficult to cover all aspects in a single research study bec
Explain the Techniques of Surgical Correction ? Techniques of Surgical Correction proximal Dissection (De Bakey type I and IZ or Stanford A) Monitoring lines are insert
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