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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Water soluble Vitamins - B complex Vitamins and Vitamin C? Vitamins are essential nutrients found in foods. The requirements are small but they perform specific and vit
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1. Fats promote absorption of fat soluble vitamins like vitamin A, D, E and K. Patients of cystic fibrosis often absorb fat poorly and are at-risk for fat-soluble vitamin deficienc
How different is the fish epidermis from the amphibian epidermis? The fish epidermis is very thin and having mucus-secreting cells. The fish skin does not present keratin. The
what is the largest nerve centre
Q. What are the characteristics of protozoans that make them resemble animals? Protozoans are unicellular beings with some related characteristics to animal cells. In compar
What are the specialized structures that help the adhesion between cells? The structures responsible for the union of the epithelial cells are known as cell junctions. The main
Explain Tertiary structure and Quaternary structure of proteins Tertiary structure Secondary structure, in turn, folds back and bonds to itself in a three-dimensional mann
ATP is DIRECTLY required in which of the following processes in muscle? A. Net flux of sodium ions from intracellular space to extracellular space. B. Detachment of myosin h
Cobalt Cobalt functions as a component of vitamin B12 (cobalamin). Ruminants are not dependent on a dietary source of vitamin B12 because ruminal microorganisms can synthesize B
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