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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Find the equilibrium molality of solute species? Consider a hypothetical system in which two aqueous solutions are separated by a semipermeable membrane. Solution α is prepared
Phosphorylation of glucose Glucose is converted to glucose-6-phosphate since phosphorylated intermediates do not readily penetrate cell membrane and this commits glucose t
What are the main intraspecific ecological interactions? The main harmonious intraspecific ecological interactions are colonies and societies. The major inharmonious intraspeci
Explain about the Zygomycota - Fungi? Fungi belonging to zygomycota are called zygomycetes. Hyphae in this sub-class are coenocytic. Asexual spores develop in sporangia at the
Describe about Acyanotic Congenital Heart Disease ? The following lesions will be discussed here: Left to right shunts: Atrial Septal Defect (ASD), Ventricular Septal Defect
In certain instances differences in the morphology of genitalia between species make it impossible for normal mating to occur, and sympatric species tend to remain isolated. When t
Q. Where can RNA are found within cells? In the eukaryote cell nucleus the RNA can be found to dispersed in the nuclear fluid, along with the DNA, and as the main constituent o
Equine infectious anaemia Equine infectious anemia (EIA), an acute or chronic viral disease of the equines, shows intermittent fever, loss of weight, depression, progressive w
Explain about the Brownian ratchets and molecular motors ? How work is carried out by molecules within biological cells is essential to understanding how cells function. A clue
What does an anther have on it? The anther is the part of the flower that keeps the pollen. This and the filament both make up the stamen.
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