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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define essentiality of Sulphur for growth of micro-organism? Sulphur is a part of some amino acids, some carbohydrates, biotin and thiamine. It is obtained either as elemental
Ontogeny: This is the study of history of an individual. Ontogeny is also known as ontogenesis and morphogenesis. Ontogeny describes the origin or the development of an organism -
M e t h ods of curing Curing ingredients are applied in dry form or as a solution of curing ingredients in water (pickle or brine). The combination cure is applied in the i
Define General Purpose and Specialized Culture Media? General purpose media - These support the growth of many microorganisms. Example: nutrient agar, trypic soy agar etc. S
Population Dispersal Population dispersal is the movement of individuals into or out of the population or the population area. It occurs in three following ways in a popu
A simple method used to identify areas of high conservation priority is the selection of countries according to highest species richness. Among the top 12 countries identified this
What are plant cell vacuoles? What are their functions? How is the covering membrane of the vacuoles called? Ans) Plant cell vacuoles are cell structures delimited by membranes
#question.what are the organs of respiration in the lwer form of animals .
how do you define sex
Define Interaction of pyridoxine with Ascorbic acid and Leucine? Ascorbic acid: Vitamin B 6 metabolism increases with. Higher levels of vitamin C intake. Whole blood a
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