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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
These are areas where woody shrubs predominate rather then trees. In regions with a Mediterranean type of climate i .e., hot dry summers and cool wet winters, shrubs grow close tog
how parasitic protozoans exibit saprophtic mode of nutrition
I nfectious coryza A highly infectious bacterial disease of chickens caused by Hemop hilus paragallinarum, is characterized by catarrhal inflammation of the upper respirator
Q. Define Accuracy of Stress Echocardiography? The accuracy of stress echo tests for the detection of coronary artery disease is expressed as the sensitivity and specificity of
Cardiac surgical intervention has an increasingly important role in the treatment of intracardiac complications of endocarditis. Retrospective data suggest that mortality is unacce
Define the Dietary and Lifestyle Modifications - Obesity? The management of obesity basically comprises the following three- pronged approach. a) Dietary modifications b)
Why is the plant life cycle known as alternation of generations? The plant life cycle is called as alternation of generations because in this cycle there are two dissimilar for
Explain Protostomes vs. Deuterostomes breifly? Branching Evolutionary Lines : It appears that the Coelomate animals separated into two divergent lines of evolution in terms o
How to Promote Physical Activity to combat from Obesity? For many children, low physical activity rather than excessive energy intake is the primary culprit. Management of obes
Why Ascending Method of Paper Chromatography is Preferred? The ascending method is preferred because of the simplicity of the set up. In actual method the substance to be separ
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