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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Why is ATP known as the energy currency of body?
Define Effect of Enery on quality and quantity of human milk? Energy: In case of chronic under nutrition, an association between postpartum weight loss and lower energy transfe
Q. How Drying used for sterilization? Moisture is essential for bacteria, drying therefore has a deleterious effect on most bacteria. Spontaneous drying can often kill bacteria
habitat of protozoa .
Explain Gastric Bypass - surgical management for obesity? Surgery is the current 'gold standard' for bariatric surgical procedures. It involves use of a stapling device to crea
SURGERY FOR CORONARY ARTERY DISEASE : Stenotic coronary artery disease (CAD) is caused by the thickening and narrowing of the coronary arteries (Atherosclerosis). Initially it cau
Dormancy - Plant Growth Substances Dormancy can be defined as a state of suspended growth and metabolism. When most plants are exposed to seasonal periods of very inclement we
Q. Write the meaning of Hyperglycemia? Hyperglycemia is a Greek term: hyper -meaning excessive; glyc - meaning sweet; and emia- means "of the blood". It is a condition in whic
Just definition
Slow creeping
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