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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Which of the following variations on translation would be most disadvantageous for a cell? A) translating polypeptides directly from DNA B) using fewer kinds of tRNA C) having only
discuss why obelia is considered to be a special organism in zoology
How would consuming fewer than 1,200 kcalories per day impact metabolism.
differences of plants and animals in terms of form and structure?
Q. In which period of meiosis does the pairing of homologous chromosomes occur? The pairing of homologous chromosomes is a very important step for meiosis because the rightness
1. Hazard Is An Extreme Event Whereas Disaster Is Its Consequence. 2. A hazard can occur without human interference but it may become a disaster on account of careless
Define the Recommended Dietary Allowance for Vitamin B 12 (RDA)? Vitamin B 12 deficiency is common in true vegans who can be treated with small doses since the daily requirem
What do you mean by Advanced characters The traits, or characteristics, that an animal has that are not ancestral to the taxon. They appear later in the evolutionary history of
Explain the Executive Functions - Psychological tests Executive functions are those involved in the planning, organisation, regulation and monitoring of goal directed behaviour
explain in brief about phyletic lineage?
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