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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Why is water conservation in c3 c4 and cam plants important? Cam stands for Crassulacean acid metabolism. C3 and C4 conserve less water than Cam plants. Actually, C4 plan
Explain Gastric Bypass - surgical management for obesity? Surgery is the current 'gold standard' for bariatric surgical procedures. It involves use of a stapling device to crea
Q. What are the major negative ions found in living beings? The main anions found in living beings are the chlorine anion (Cl - ), the sulphate anion (SO - ), the nitrate ani
Gene expression is controlled by chromatin state, _______ and _________. a. transcription factors and epigenetic regulators b. polymerases and control proteins c. delta and tau pro
What is omnispective classification
As the functions of individual genes become known, the power of this new biology can be used to change organisms in predictable and desirable pathways. Organisms which have been c
Protozoa classification
VACUOLES They are of 4 types - 1. Sap Vacuole - Outer membrane of sap vacuole is called T onoplast . Sap vacuole contains amino sugars, carotenoids, proteins, m
Define Classification of foods based on pH? Of these, the nature of the food, primarily the pH of food, is the most significant determinant of how severely the food will be pro
Primary Production - Ecosystem Energy accumulated by plants during photosynthesis is called production or more specifically primary production. It is the first and the basic f
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