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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What are the hydrophobic molecules (or hydrophobic molecular regions)? What are the hydrophilic molecules? How can they be characterized in relation to their polarity? Hydro
State about Bone physiology Bone physiology that is most relevant to the study of mechanically mediated bone adaptation and its relevance in implant dentistry. These aspects in
Phylum Placozoa The phylum Placozoa contains a single species of a minute marine animal Trichoplax adharens composed of a dorsal and ventral epithelial layer enclosing loose m
In yeast, a new mutation called suc was recently discovered. The suc mutant cannot use sucrose as a source of carbon, and will not grow if the culture medium contains only sucrose
What do you understand by Polyp? The sessile, asexual stage in the cnidarian life cycle. In some species they are independent organisms; in others, they form colonies where som
what are the examples of parasitic plants
In humans, attached earlobes are a dominant feature over free earlobes while hypertrichosis of the ear is a holandric (Y-linked) feature. A person with attached earlobes and extens
Q. What is the criterion used to classify hosts as intermediate hosts or as definitive hosts? The criterion used to categorize hosts as intermediate hosts or as definitive host
iwant to know all about phylem protozoa?
Discuss why Obelia is considered to be of special interest in Zoology as an animal showing an intermediate grade of organization.
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