Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Disbeliefs about insulin injection, Some people have disbeliefs regarding i...

Some people have disbeliefs regarding insulin injection. You have to find out if there are any disbeliefs and try to explain the importance of insulin injection. Do not force the p

Determine the occurrence of vitamin A, Determine the Occurrence of vitamin ...

Determine the Occurrence of vitamin A In the vegetable kingdom, vitamin A  probably occurs in the form of its provitamins which belong to the group of carotenes. Carrots, spina

Explain the type I diabetes mellitus, What the difference is between type I...

What the difference is between type I diabetes mellitus and type II diabetes mellitus? Type I diabetes, also called as juvenile diabetes, or insulin-dependent diabetes (this na

Protonephridia - excretion, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Transmission electron microscopy, Transmission electron microscopy: Ar...

Transmission electron microscopy: Around 1931-32 two German scientists. Knoll and Ruska. Invented transmission electron microscopy, and built the first transmission electron m

ANIMAL KINGDOM , CLASSIFICATION OF PHYLUM PLATYHELMINTHES..

CLASSIFICATION OF PHYLUM PLATYHELMINTHES..

How d-glucose differs from d-galactose, D-glucose differs from D-galactose ...

D-glucose differs from D-galactose only in the arrangement around carbon 4. Therefore D-glucose and D-galactose are- Select one: a. enantiomers b. epimers c. mirror ima

Determine the evaluation of the bone, Evaluation of the bone Evaluation...

Evaluation of the bone Evaluation of the bone -implant interface needs to be done by the following: Failures in Implant Dentistry 1) Implant mobility and discomfort 2)

What is the etiology of cholera, Q. What is the etiology of cholera? T...

Q. What is the etiology of cholera? The most common cause of cholera is consumption of food or drinking water that has been contaminated with the bacteria. It is important to

Amoeba, figure of contractile vacuole of amoeba

figure of contractile vacuole of amoeba

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd