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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the Recommended Dietary Allowances - Nutrition? RDA: The RDA is the average daily dietary intake that is sufficient to meet the nutrient requirement of nearly all healt
Define the Indications for Root-End Resection Apicoectomy a) Persistent..... "Exactly the SAME of Curettage except the biopsy. b) When the apical portion of the root canal s
what is the bet way to asses energy production in the body of an athlete?
Animals - Rapidly Flowing Waters In the exposed rock surface habitats only those organisms are found which have efficient mechanisms for staying in one place. In fact despite
(i) Effect on Human Health: Air pollution causes many respiratory problems in human being. (1) Dust, soot and smog causes several respiratory troubles such as bronc
Define Ascorbic acid content of white blood cells? The ascorbic acid content of white blood cells and platelets is a good index of the tissue levels of ascorbic acid. The level
Nucleosides : compounds formed from a nitrogenousbase and a penstose sugar.
what is the correct classification of phylum mollasca
Blastulation explain
What respectively are zygotic meiosis, gametic meiosis and sporic meiosis? Zygotic meiosis is the one that happens in the haplontic haplobiontic life cycle. Gametes from adult
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