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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Why are salt and sugar used in the production of dried meat and dried fruits? Substances that maintain a highly hypertonic environment, such as sugar and salt, are used in the
Can enhancers work in Trans? That is, can an enhancer on one piece of DNA activate a promoter on another piece of DNA? Propose an experiment to test this. Be sure to discuss any ap
In hormonal terms why does menses occur? Menses is the endometrial monthly desquamation that happens as the estrogen and progesterone levels fall after the regression of the co
Q. Do plants present only sexual reproduction? There are asexual forms of reproduction in plants few naturally detached pieces of root, leaves or limbs develop into another com
buffalo can be dies due ashyxia
Presenting Features Anterior abdominal wall and anterior bladder wall is deficient and on the surface everted posterior wall of bladder with two ureteric orifices is seen.
State the Diagnosis and prevention of VIBRIO PARAHAEMOLYTICUS Diagnosis: Diagnosis of gastroenteritis caused by this organism is made by culturing the organism from the diarrh
Metamorphosis The phenomenon of metamorphosis is described as a process during development which involves a remarkable change in morphology and physiology of the larva, so tha
STRUCTURE Golgi body consists of :- (1 ) Cisternae These are plate like unbranched structure with dilated ends. One golgi body has 4-8 cisternae. Mono cisternal
who is the father of science
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