Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Determine the solubility of micronutrient cations, Determine the solubility...

Determine the solubility of micronutrient cations The solubility of micronutrient cations is at a maximum at low pH values. As the pH increases the ions of these elements first

Describe the functional problems of implant failures, Functional Problems o...

Functional Problems of implant failures The masticatory efficiency of the implant supported prosthesis depends on the anchorage related factors and the prosthesis related fact

Physical map, Physical map is the map of locations of the identifiable lan...

Physical map is the map of locations of the identifiable landmarks on DNA (for example restriction of the enzyme cutting sites, genes), regardless of their inheritance. Distance i

How many cellular nuclei does the pollen tube have, How many cellular nucle...

How many cellular nuclei does the pollen tube of angiosperms have? What is the ploidy of each of these nuclei? The pollen tube that is the mature male gametophyte of angiosperm

What is behavioural neurology, What is Behavioural neurology Behavioura...

What is Behavioural neurology Behavioural neurology contains the study of disorders of mood, personality, intelligence, perception and arousal and is concerned with the structu

Artificial parthenogenesis, Artificial Parthenogenesis In various cas...

Artificial Parthenogenesis In various cases, the eggs that normally develop only after fertilisation can be experimentally induced to develop parthenogenetically by specific

Define advantages of using bacteria as a source of protein, Define Advantag...

Define Advantages of using Bacteria as a source of protein? Advantages of using Bacteria as a source of protein are as follow: 1. High yield of protein (60-80%) 2.

Opiate narcotics - psychological drug dependence, OPI A T E NARCOTICS - ...

OPI A T E NARCOTICS - They suppress brain activity and relieve pain popularly known as pain killers. They are sedative and astringent in nature. An astringent cause

Explain scope of food science and technology as a subject, Explain Scope of...

Explain Scope of Food Science and Technology as a Subject? Food science and technology has developed as a discipline to systematically organize and link various kinds of knowle

ASSINMENT, HISTORY ON CLASSIFICATION IN BIOLOGY

HISTORY ON CLASSIFICATION IN BIOLOGY

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd