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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the working of Conical Flasks? Conical flasks of different volumes can be used for media preparation and also for culturing large amount of microorganisms in liquid med
Reptiles Heart is incomplete 4 chambered, ventricles are not divided completely 2 auricels & 2 ventricles. Sinus venosus present, Truncus artiorsus absent [In lizzard fo
merits and demerits system of classification by Linnaeus
. Explain why the traditional classification of unicellular eukaryotes as ‘protozoa’ or ‘protists’ is invalid in terms of modern systematics and evolutionary theory. Why are trad
What are the main substances transferred from the mother to the fetus through the placenta? And from the fetus to the mother? From the mother to the fetus the major transferre
Q. Adverse effect to intestinal mucosa? Alcohol: Alcohol is known to cause damage to intestinal mucosa independent of gastric acid content. Thus high amounts are not advised.
Explain the Procedure for Preparation of Culture Media? Now carry out the exercise following the steps given herewith: 1. To prepare potato dextrose medium, first, peel off
Question 1 Discuss how you would perform mounting of stained sections. List commonly used mounting media in histopathology laboratory. Add a note on advantages and disadvantages o
Q. What are the three stages into which the cell respiration is divided? The three stages of aerobic cell respiration are glycolysis, Krebs cycle and respiratory chain also kno
Q. What is continuous full thickness incision? 2. Continuous full - thickness incisions are indicated for a variety of situations outlined below. This type of incision may be u
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