Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Agro industrial-mineral biofortification of plants, Mineral biofortificatio...

Mineral biofortification of plants One sustainable agricultural approach to reducing the mineral deficiencies in livestock animals is to enrich major staple food crops (rice,

Define surface properties of proteins, Define Surface properties of Protein...

Define Surface properties of Proteins? The surface properties relates primarily to surface tension, emulsification and foaming characteristics of proteins, which are discussed

Functions of cell wall, FUNCTIONS OF CELL WALL 1.       Provides shape ...

FUNCTIONS OF CELL WALL 1.       Provides shape to plant cell rigidity to cells. 2.       Functions as a barrier to entry of pathogens into the cells. 3.       Provides pr

What are the three main limiting factors of photosynthesis, What are the th...

What are the three main limiting factors of photosynthesis? The three major limiting factors of photosynthesis process are light intensity, carbon dioxide concentration and tem

Testing a leaf for statch, State four steps to be followed to test for star...

State four steps to be followed to test for starch in a leaf

Sp and spp., what is the deference between sp. and spp.?

what is the deference between sp. and spp.?

Explain thermal destruction of enzymes during sterilization, Explain about ...

Explain about Thermal Destruction of Enzymes during Sterilization? Before we move on to canning, we need to emphasize here that like thermal destruction of microorganisms, ther

Cardiac branches and cardiac plexuses, The vagus gives of one superior cerv...

The vagus gives of one superior cervical cardiac branch and an inferior cervical cardiac branch in the neck. These branches descend into the thorax and take part in performing the

Describe directly and indirectly modes of transcription , 1.  A completely ...

1.  A completely new protein has been synthesized by a research lab. You are inadvertently exposed to it and have an allergic response. What type of antibody initiates this respons

Maintain nutrition in diarrhoea, Maintain nutrition Your responsibilit...

Maintain nutrition Your responsibility as a nurse is to take care of nutrition and prevent the child from starvation. Breast feeding'should be continued during the diarrhoea e

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd