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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Pharmacognosy : This is the study of medicinal plants. Pharmacognosy is a kind of the study of medicines which are derived from natural sources. The American Society of Pharmacogn
Some ecosystems possess relatively large number of species as compared to others. This is particularly true of tropical forests which exhibit a very rich-biodiversity. For example,
Competition - Processes in Succession The aggregation of individuals in an area leads to inter specific and intra specific competition. The competition is usually for
What is Total Cavo Pulmonary Connections (TCPC)-Lateral Tunnel Operation? Cardiopulinonary bypass is instituted by cannulation of ascending aorta, SVC innominate vein junction
Valvular heart diseases : Congenital and acquired valvular heart diseases often require surgical intervention. 1 is well known that conservative surgery by valve repair gives bet
Diet : Pre-operatively, patients are on low calorie, low fat diet. However in the immediate post-operative period, strict dieting is not advisable. They need good nutrition for p
What is cytoskeleton? What are its main constituents in animal cells? Ans) Cytoskeleton is the cytoplasmic structure that handles the cell, keeps its shape and fixates and moves
Explain the Localized Aggressive Periodontitis Localized Aggressive Periodontitis: It has been in literature that transmission of peridontopathic microorganisms from periodon
Illustrate the Horizons in detail 'O' is the organic horizon formed from t he organic litter derived from plants and animals and fresh or partially decomposed organic materi
If one determines the concentration of cortisol in the blood plasma to be 10 ugrams/ml and that in a 24 hour period a person excretes 10 mgs of cortisol, calculate the volume of bl
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