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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Class of Subphylum Uniramia - Symphyla Symphyla is yet other small myriapodous group that includes around 160 described species. These are also soil living forms and live in l
In holly, serrated leaves are dominant to smooth-edged leaves, and red berries are dominant to green berries. Two holly plants heterozygous for leaf edge shape and berry color are
Which of the following is true for a G-protein? A. After the ATP-ase of the alpha subunit of a G protein converts the ATP bound to the alpha subunit to ADP and inorganic phosph
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Which of the following are true for ventilation? A. The problems with ventilation induced by injection of curare occur because of the drug's direct action on nicotinic ACh Rec
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Amino acid sequence is also known as the primary structure of a protein/polypeptide; the series of amino acids in a protein/polypeptide controlled by the series of DNA bases.
Define the Cooper's Run (12 minute run) Method? To undertake this test, a 400 metre running track is required-marked every 100 m and a stop watch. The test comprises of noticin
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Define Methods of Quantitative Analysis and Colorimetry? Many methods of quantitative analysis are based upon the production of coloured solutions in such a way that the intens
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