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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
If you observe symptoms of overall wilting, stunting, and thinning of the leaves, would you expect a foliar or root disease? Why?
Discovery of Plant Hormones To date, five major classes of plant hormones have been discovered namely auxins, gibberellins, cytokinins, abscisic acid and ethylene. It is possi
write the charecteristics of lake ecosystem
Risk during crown removal -Tooth inadvertently extracted using a crown\bridge remover . -Endodontic therapy was performed , and the tooth was replanted , a procedure known
Corpus Cardiacum - Endocrine Organs We before mentioned that corpus cardiacum (pl. corpora cardiaca) is a neurohemal organ in insects. It is gland of neural origin. They are c
Diffusion of Gases Gases diffuse from areas of higher partial pressure to areas of lower partial pressure. The diffusion along the partial pressure gradient ceases only when
W A TER Occurence Water is the most abundant substance, covering 70% of earth's surface. It constitutes 60-90% of living cells. 5-6% in tooth e
Q. What is the role of the ozone layer for living beings? The Ozone, O3, is a gas of the atmosphere that filters ultraviolet radiation from the sun disallowing most of that rad
Functions of Stomata The cuticle forms outermost surface of the leaf and offers resistance to the evaporation of water vapour and entry of carbon dioxide necessary for photosy
Calculate the pH: 300mL of 0.25M sodium ascorbate plus 150mL of 0.2M HCl (the pKa of ascorbic acid is 4.04) okay, what's the conjugate acid and base of this problem? Can someone wa
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