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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Which is the biological molecule that contains the genetic information that is transmitted hereditarily and controls the cellular functioning? The hereditary molecule that cont
Buffalo-pox The disease is caused by an orthopox virus, closely related to the vaccinia virus. It is not clear whether it should be considered a separate virus or not. By RE m
A professor wonders whether good study habits lead to better grades in the final exam. Formulate a specific hypothesis and then design an experiment to test it. In your answer outl
What are the differences between the two domain
IN WHAT ASPECT ARE CNIDARIANS SIMILAR TO PROTOZOANS
What are complementary genes? Does this inheritance pattern obey Mendel's second law? Complementary genes are the different genes that act together to determine a given phenoty
Extended point within pulp chamber: Gripping action: - Steglitz forceps or pin pilers. - Access cavity flooded with chloroform to dissolve the sealer. - Grasp the
It is important to recognise underlying causes and precipitating factors of heart failure for its appropriate management. That would also help in prevention and treatment of heart
Phylum Ascomycetes 1) Sexual reproduction is by conjugation and is followed by the formation of ascospores inside a sac called ascus. 2) The asci may be grouped together to
CHLOROPLASTS Schimper (1883) coined the term chloroplastid for green plastids. Mayer called them Autoplast. Chloroplast name proposed by Erera. Green plastids wi
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