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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are the intermediate and the definitive hosts of the plasmodium? In the life cycle of the plasmodium humans are the middle hosts (where asexual reproduction takes place) a
Japanese encephalitis Japanese encephalitis (JE) is a mosquito-borne encephalitis and is caused by flavivirus belonged to the family Flaviviridae. It is a zoonotic disease, i
How does the addition of a watery secretion to the contents of the gut help in the process of digestion?
Explain the Sticky Films - Food Microbiology? Sticky films or tape are pressed against the surface to be assessed. Exposed film/tape is then pressed on the agar plate and analy
Q. What are the morphological, chemical and functional similarities and differences between peroxisomes and lysosomes? Similarities: Peroxisomes and lysosomes are small membran
Why do diabetic patients often undergo dietary sugar restriction? What are the main complications of diabetes mellitus? Diabetic patients are often advised to ingest fewer carb
Explain Feeding Schedule in Infancy? 1) Milk feed 5-6 times/day. Continue breast-feeding. Exclusive breast-feeding up to 6 months even during illness. Follow demand feeding.
As coenzymes participate in a variety of functions, they can be classified broadly into two groups: i) Hydrogen transferring coenzymes, and ii) Group transferring coenzym
what are the organs of respiration in the lower form of animals?
A healthy person is on a diet that consists of very small amounts of water. A. This person will have high water permeability across the luminal membranes of the medullary colle
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