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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
There are 3 types of DNA sequences. Genomic DNA comes from the genome and haves both genes and extragenic material. cDNA is reverse transcribed from mRNA and corresponds only
Class Hirudinea - Classification of Coelom These are leeches. Number of body segments of these types of animals is fixed; usually there are 34 segments in a few groups there m
the appendages of arthropods; a. may serve as walking legs, b. may be modified into atennae, c. may be modified into large pincers, or d. all of the above
When the Balance Sheet column totals don't agree on the first attempt work backward through the process used in preparing the work sheet exclusively take the following steps until
How different are gymnosperms from pteridophytes and bryophytes? Gymnosperms are not cryptogamic as pteridophytes and bryophytes are. They are phanerogamic and so they form see
Analyse the model that highlights the factors which influence the environmental impact of a population
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The nutrition care process acknowledges the common dimensions of practice by the following: Defining a common language that allows nutrition practice to be more measurable,
advantages and disadvantages of protozoa
Intergenic: Amongs the two genes; for example intergenic DNA is the DNA found amongs two genes. The term is frequently used to mean non-functional DNA (or at least DNA with no kno
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