Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Somatic embryogenesis, Somatic Embryogenesis The process of embryo de...

Somatic Embryogenesis The process of embryo development is called embryogenesis. It is not the monopoly of the egg to form an embryo. Any cell of the female gametophyte (Embr

Explain acyclovir, Explain Acyclovir Available in topical, oral, and in...

Explain Acyclovir Available in topical, oral, and intravenous (IV) formulations, acyclovir is used to treat herpes simplex virus (HSV) and varicella-zoster virus (VZV) infectio

How are birds characterized, Q. Bird identity card. How are birds character...

Q. Bird identity card. How are birds characterized according to examples of representing beings, basic morphology, respiration, skin, nitrogen waste, circulation,thermal control an

Omnispective classification, Omnispective Classification This is the ex...

Omnispective Classification This is the extension of the concept of natural classification put forward by Black Welder (1967). The approach seems quite realistic and pragmatic.

Explain the uses of isP in infant formulas, Explain the Uses of ISP in Infa...

Explain the Uses of ISP in Infant formulas Infant formulas  Infant formulas, where milk solids have been changed by soy products, are well established commercial products

Define feeding and nutritional management of dysphilgia, Define Feeding and...

Define Feeding and Nutritional Management of Dysphilgia? A multidisciplinary approach is essential for successful management of dysphilgia. Patient, nurse, physician, die titi

Protozoa., show me the schamatic diagram of chrysomoeba?

show me the schamatic diagram of chrysomoeba?

What are the catalysts, Q. What are the catalysts? Catalysts are substa...

Q. What are the catalysts? Catalysts are substances that decrease the activation energy of a chemical reaction, facilitating it or making it energetically viable. The catalyst

Define essentiality of nitrogen for growth of micro-organism, Define Essent...

Define Essentiality of Nitrogen for Growth of Micro-Organism? Nitrogen is an essential atom in certain cellular macromolecules, e.g. amino acids, purines, pyrimidines, enzymes,

Explain venous pulsation, Explain venous pulsation? Venous Pulsation: N...

Explain venous pulsation? Venous Pulsation: Normally the jugular venous pulsation faithfully reflects the pressure changes in right atrium. It is described as a, x, c, x, v, y,

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd