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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Adverse Effects of ganciclovir Ganciclovir is teratogenic, carcinogenic and mutagenic and causes aspermatogenesis in animals. Granulocytopenia and thrombocytopenia, whi
classification of invertebrate
How is retaining wall works? A retaining wall together with the backfill the wall retains and the soil that supports the wall is a highly indeterminate system. The magnitudes o
on line mitochondria lab
Q. From which germ layer do blood cells originate? What are other organs and tissues made from that germ layer? Blood cells have a mesodermal embryonic origin and other organs
Explain how human activities can cause an imbalance in biogeochemical cycling and lead to problems such as cultural eutrophication and fish kills
The stepped steel shaft illustrated in Figure is subjected to a torque (T) at the free end and a torque (2T) in the opposite direction at the junction of the two sizes.
Q. Classification of plant on the basis of their resemblances? Pliny seemed to have little interest in the classification of plant on the basis of their resemblances. He classi
Define Post-surgical Nutrition Support for CHD Patient? Nutrition support should be started as soon as possible. If oral not possible, enteral nutrition support should be pr
What is the Importance of Vitamin K The site of action of vitamin K activity is the highly complex mechanism of blood coagulation. Due to its effect on prothrombin, vitamin K i
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