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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How does the structure of a chloroplast enable it to build up a concentration gradient of protons? Chloroplasts have an inner membrane system having of thylakoids. The pumpin
The major ecological imbalance on Earth is between the human species and the rest of Earth's biota. What are two specific actions we could do to achieve a balance (for example, rec
Hydrophily - Cross-pollination All hydrophytes are not necessarily pollinated by water. In fact most of the aquatic plant are anemophilous, e.g. Alisma, Nymphaea. Like anemoph
are viruses cellular organisms
hmp shunt introduction types significant
"Diversity is good" is one of the axioms of conservation biology. However in absentia of historical information, why is noting higher diversity in a system NOT the best way to eval
What are the two mains divisions of the chordate phylum? Chordates are separated into protochordates (cephalochordates and urochordates) and vertebrates. Life Kingdoms Revi
Define Fluids requirement to avoid underweight problem? Take fluids only after a meal instead of with or before meals so that food intake is not reduced. High calorie nourishin
Lakes - Lentic Ecosystems Most lakes occur in regions which have recently been subjected to geological changes; say within the past 20,000 years. However, a few lakes, such a
Assessment and Nursing Care Plans Clustered Case Study Mr. Smith is an 83 year old gentleman (DOB 1.2.1928), who lives alone in his home on a cattle farm west of Ravenshoe.
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