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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How Drugs Increase Food Intake? You must understand that drugs may also increase food intake. This is because they can: Stimulate appetite : This is a common side effect
THYMU S GLAND Flat, bilobed gland located in the mediastinal space in front of heart. Well developed in new born child and grows upto puberty but starts degenerating the
An ecosystem consists of two types of components: (a) Abiotic components: This includes the non-living components of the ecosystem. These are physical and chemical fact
Q. Discuss the role of dental implants in orthodontic anchorage? The increasing popularity of dental implants has led to its use for orthodontic anchorage. Conventional means o
WHAT IS THERE BETWEEN THE TRACHEA OF COCKROACH AND RABBIT
What are plant hormones? Plant hormones, also known as phytohormones, are substances that control the embryonic development and the growth of the adult plant.
Contamination of the Implant Body before Insertion The implant may be contaminated by manufacturing error, by the operator, from non titanium instrumentation and by the bacteri
Explain the Analysis of Food Samples? Now, in this practical, we shall deal with the endogenous and exogeneous microorganisms which are present on the food as contaminants. We
Q. Show the Use of Incineration for sterilization? This is an excellent method for rapidly destroying materials such as soiled dressings, animal carcasses, bedding and patholog
Coccidioidomycosis Coccidioidomycosis is a soil-borne infection of pet, domestic, wild animals and man. The disease is caused by Coccidioides immitis, a dimorphic fungus wh
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