Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What is echocardiography, Q. What is Echocardiography? Ans. Echoca...

Q. What is Echocardiography? Ans. Echocardiography has become an established and powerful tool for diagnosing presence of CAD and defining its consequence. It can help in

How water content influence the rs content, How Water content Influence the...

How Water content Influence the RS content? The yield of RS formed in heat-moisture treatment is closely related to water content, which may be an inherent component of food or

Different osmotic pressures, Q. Can solutions with the same concentration o...

Q. Can solutions with the same concentration of diverse solutes have different osmotic pressures? The osmotic pressure of a solution does not depend on the nature of the solute

Define the effect of breastfeeding on mother, Normal 0 false ...

Normal 0 false false false EN-IN X-NONE X-NONE

Describe how many bones are there in human foot, Describe how many bones ar...

Describe how many bones are there in human foot The human foot is a great mechanical marvel. It has 26 bones, 29 joints, and 42, various ligaments, a sensitive and protective s

Zoogly, are protozoans primitive to life .

are protozoans primitive to life .

Basic properties to proteins, Define Basic Properties To Proteins, To Be a ...

Define Basic Properties To Proteins, To Be a Good Foaming Agent? A protein must: a) Be able to rapidly absorb at the air-water interface during whipping, b) Undergo rapid

Explain procedure for the use of light microscope, Explain Procedure for th...

Explain Procedure for the use of Light Microscope? Now carry out the exercise following the steps enumerated herewith. 1. Place the microscopic slide with any specimen on th

explain alternative processing, Alternative polyadenylation sites Exa...

Alternative polyadenylation sites Exact pre-mRNAs hold more than single group of signal sequences for 3' end polyadenylation and cleavage.   In  some  cases,  the  area  of  t

Phylum Coelenterata, What are the classes in Phylum coelenterata?

What are the classes in Phylum coelenterata?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd