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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
The ability to sequence DNA has led to a revolution in molecular biology. a) Describe the principles of DNA sequencing. b) A single sequencing reaction yields at best 1500 ba
Is gene a triplet of consecutive DNA nucleotides A gene isn't a triplet of DNA nucleotides with their respective nitrogen-containing bases, like CGT or AAG. The nucleotide trip
What is the function of the citric acid cycle? The hnction ofthe citric acid cycle can be discussed as follows. The intermediates of citric acid cycle are used as precursor
COMMON EQUINE DISEASES - 1 . INFLUENZA OR PINK EYE - Pink eyes. Swallon eye lids. 2. STRANGLES - Fever, dullness, depression, nasal discharge, lumpy a
Duck plague Duck plague, caused by a member of Herpesviridae, has world wide distribution and occurs among domestic and wild ducks, geese, swans and waterfowls. Epidemiolo
Start with a cell with chromosome number 2n=6, and having two acrocentric pairs and one metacentric pair of chromosomes. Draw the cell in metaphase I. Indicate one pair of sister c
structural adaptation of mammalian alimentary canal
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What are nutritional Care related to Neurological Disorder? Let us then move on to the nutritional care. The important goals of nutritional care are to: prevent further
Hygroscopic Coeflicient To have a more complete picture of soil moisture relations, let us take a soil sample and oven dry it for 24 hours at 11 o C. Now if this soil is kept i
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