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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What are the functions of the osseous tissue? The main functions of the osseous tissue that are to provide structural rigidity to the body and to delineate the spatial posit
Q. How can the hypothesis that asserts that chloroplasts as well as mitochondria were primitive prokaryotes that related in mutualism with primitive anaerobic eukaryotic cells be c
Vital Signs of clinical evaluation Patient's clinical evaluation starts with the examination of certain vital signs which are: B.P. (Take average of two regarding with atlea
What do you understand by Flagella. A cellular hairlike locomotory structure which comprise an extension of the plasma membrane surrounding a 9+2 organization of microtubules.
Population Growth - Population Parameters and Regulation The size of a population depends upon the balance between natality and immigration through which individuals are added
Ask questionImagine you are studying a group of amphibian species that vary in their habitats, some living in dense murky water, and others living in very clear ponds. What kind of
Class of Subphylum Uniramia - Chilopoda Chilopoda have the centipedes. The class contains some 2500 species that have been described so far. They inhabit both of the tropical
What do enzymes do, and how? How is enzyme activity regulated in cells? How do ATPases u se ATP to make reactions go?
Effects on Aquatic Organisms - Water Pollution Toxic water pollutants such as metals, pesticides, insecticides and chemicals affect aquatic species directly whereas the non-t
Can you use a regular syringe instead of a gas syringe in a lab
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