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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
MYCOPLASMOSIS Mycoplasma organisms are the smallest free living, lack of cell-wall and bounded by a triple layer of plasma membrane. The organisms are pleuomorphic in shape, viz.
What is the activation center of an enzyme? Is it the key or the lock of the lock and key model? The activation center is a region of the enzyme formed by its spatial conformat
newer trends of animal taxonomy
Myoglobin We can build up the structure of haemoglobin by considering a single unit or monomeric form caned myoglobin. This consists of a single polypeptide chain the globin i
Why is cannibalism an inharmonious intraspecific ecological interaction? In cannibalism an individual eats other of the same species (occurs in some insects and arachnids). Sin
ONSE T OF PUBERTY IN FEMALE - Attains at the age of 13 by estrogen hormone. It includes - 1. Growt h of breasts 2. Growth
Determine about the term - Copper Copper is known to act as an electron carrier in enzymes which bring about oxidation - reduction and regulates respiratory activity in plant
Q. What are the anatomical relationships between the organs of the female reproductive system from the external vulva to the ovaries? The external female genitalia are called t
Which is the cell organelle that is specialized in aerobic respiration? The cell organelles that are specialized in aerobic respiration are the mitochondria. Cell Respiratio
Parthenogenesis The development of egg to form an animal without fertilization is called as parthnogenesis. Parthenogenesis was discovered by Charles Bonnet in the egg of se
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