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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Pyramid of Numbers - Ecological Pyramids A graphic representation of the total number of individuals of different species belonging to each trophic level in an ecosystem is kn
#question.unique features of amoeba.
What are the general symptoms of brain tumors The general symptoms of brain tumors, which result from increased intracranial pressure, include headache, vomiting, swelling of t
What are some examples of human cells that produce proteins for exportation? Which cytoplasmic organelle is expected to be well-developed and abundant in those cells? Specializ
Celia is a nurse in a geriatric ward. She noticed that older persons in her care are having problems sleeping at night. She decided to introduce non-pharmocologic ways of relaxat
Q. What is the name of the cells capable of making gametes? What is the ploidy of these gamete-forming cells? The cells that form gametes are the germ cells as opposed to the s
Define Criteria for Assessment of Niacin Status? Niacin status can be monitored by daily urinary excretion of methylated metabolites, especially the ratio of the 2-pyridone to
Which type Ribosome occurs exclusivley in Mitocondria?
You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate
NEO-LAMARCKISM Modem modified form of Lamarck theory is regarded as Neo-Lamarckism. Mc Dougall (1938) found that time taken for training the mice was reducing gradually
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