Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

rumen protection of nutrient (bypass nutrients) technology, Rumen protecti...

Rumen protection of nutrient (bypass nutrients) technology The amino acid and energy requirements of medium and high yielding cows and buffaloes are not fully met from the micr

Elaborate evolution by natural selection , Elaborate Evolution by Natural S...

Elaborate Evolution by Natural Selection ? The conelaboratecept of natural selection lies at the heart of Darwin's Theory of Evolution. Darwin understood that plants and animal

Cells, Suppose you were planning to use liposomes in an attempt to deliver ...

Suppose you were planning to use liposomes in an attempt to deliver drugs to a particular type of cell in the body, for example, a fat or muscle cell. Is there any way you might be

Cilia and flagella, CILIA AND FLAGELLA Purkinj e first observed c...

CILIA AND FLAGELLA Purkinj e first observed ciliary movement. Hodge studied detail structure of sperm flagellum. Manton studied structure of cilia in plants.

Define the insulin resistance - obesity, Define the Insulin resistance - Ob...

Define the Insulin resistance - Obesity? Insulin resistance is a condition in which your body cells cannot utilize insulin efficiently although sufficient amounts are secreted

Types of surgery, Types of Surgery :  Tricuspid valvotomy is done as an op...

Types of Surgery :  Tricuspid valvotomy is done as an open procedure at the end of surgery on mitral and/or aortic valve. It can be done on an arrested heart or on a beating heart

Determine the postovulatory phase of x menstrual cycle, Healthy human femal...

Healthy human female X is 25 years old and not pregnant.  During the postovulatory phase of X's menstrual cycle,   A.  there are high blood plasma levels of LH and FSH.

Classification of zoonoses disease , Classification of zoonoses More t...

Classification of zoonoses More than 300 zoonoses of diverse etiologies are recognized. Thus, a very large number of zoonoses call for classification, especially for teaching

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd