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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Which of the below refers to science concerned with the application of genetic principles to improvement of the human species? a) Eugenics b) Assortive mating c) Holandri
Protein structure is determined solely by a protein amino acid sequence. Should a genetically engineered protein in which the order of all amino acids is reversed therefore have th
what is the structural formula for Galactose?
Q. What are the enzymes? What is the significance of enzymes for living beings? Enzymes are proteins that are catalysts of the chemical reactions. From Chemistry it is known as
Equine encephalomyelitis Encephalomyelitis or inflammation of the brain tissue and the spinal cord, can be brought about by different viruses. Eastern-, Western- and Venezuelan
What is Hemorrhage Mild to moderate capillary ooze can readily be controlled by pressure packing. A more severe venous or, in rarer instances, an arterial bleed may require cl
Explain the Colorimetric Method Glucose Estimation? Alkaline copper reduction methods are by far the most commonly used methods for glucose estimation. This time honoured metho
Lack of iron in the photic zone of the ocean restricts the size of plankton population.Iron is what kind of factor for marine plankton?
What are Flavanols? Examples are catechin and epicatechin. You may have heard of the benefits of tea. Some of them are due to gallic acid, which is combined with epicatechin. F
Clinical symptoms of HIV Generally, a person infected with HIV does not show any apparent symptoms for a number (3 to 12) of years. Ten to fifteen percent infected people may,
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