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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
A genetic trait which is considered to be harmful in the present day might prove to be beneficial in future. For example by removing the trait for sickle cell we may increase the s
Q. Is fecundation in amphibians internal or external? In this aspect are amphibians evolutionarily proximal to fishes or to reptiles? In the majority of the amphibian species f
ACCESSORY GLANDS - 1 . PROSTATE GLAND - One in number, 4-5 lobed, it forms 15-30% of semen.Citric acid, lipid, acid phosphatase spermin, fibrinolysine are present.
Describe Protocol that are used for Endotracheal Intubation in ICU Intubation is a three person procedure; skilled assistance is mandatory: • "Top end" intubator who coordi
Agroup of realated genera are classified as-?
Tubercular Meningitis (TBM) Tubercular meningitis is a serious complication of childhood tuberculosis. It usually occurs secondarily to primary infection with tuburculosi
Explain th eobjective of intensive care? After reading this unit, you should be able to: • know how to organize an intensive care unit; • practice effective cardio pulmonary
How might the destruction of large areas of tropical rain forest have world-wide consequences? As plants consume CO 2 during photosynthesis, extensive deforestation could resu
Competition - Processes in Succession The aggregation of individuals in an area leads to inter specific and intra specific competition. The competition is usually for
Describe the Phylum Chordata in animal kingdom? This is the other group of animals along with the Echinodermata whose anus develops prior to the mouth's development in the embr
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