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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Evidence Similarities between 79° and 70°, eg Feeding activity continuous / continues for 24 hours during polar day (summer months) feeding activity more intense i
Transmission Electron Microscopy The source of illumination in a TEM is a tungsten filament that emits electrons. A vacuum is created to prevent the collision of electrons with a
What are the main functions of the bacterial flora within the human gut? Bacteria that live inside the gut have great significance in digestion. Some polysaccharides like cellu
Estimation of protein by Availability of amino acids include? Regeneration of blood and liver constituents includes Liver protein regeneration Blood protein regener
What is (a) the male gamete, and (b) the female gamete in a flowering plant? (a) The male gamete in a flowering plant is the pollen grain (strictly, the gamete is the male
define heart?
#queshition..
Determine the term - Chlorine The application of chlorides results in leaves of greater spread, a lighter green colour and with a smoother surface. However, the combustibilit
whats the difference in respiratory system in mammals,reptilia and amphibian?
Transport of Gases in Blood We know that two types of gas exchanges are constantly occurring in the animal body--one at the inter phase of the respiratory membrane and externa
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