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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
A cell with a euploid number of chromosomes contains: A.one half of a complete set of chromosomes B.several complete sets of chromosomes C.the correct number of chromosomes D.two c
What is the Speech Perception Test The subject is asked to listen to a series of 60 sounds, each of which consists of a double e digraph with varying prefixes and suffixes. The
Define the Recommended Dietary Allowance for Thiamin (RDA)? Thiamin, as it must be clear by now, is needed mainly for the metabolism of carbohydrate, branch-chained amino aci
Q. What are the structures that form the external ear? What is its function? The internal ear comprises the auricle or pinna and the auditory canal its function is to conduct t
What do numeric pyramids represent? The Numeric pyramids represent the number of individuals in each trophic level of a food chain.
what is the main excretory organ of a lizard
Addition of glycine to the physiological saline A complete motor neuron is removed from a frog and placed in a large volume of normal physiological saline. The neuron is heal
Metaphase chromosomes: Each metaphase chromosomes is a duplicated structure which consists of two sister chromatids, attached at a point called centromere or primary constric
Amniocentesis is a way of prenatal testing in which the amniotic fluid is withdrawn from the uterus by the help of a needle. The fluid and the fetal cells it contains are analyzed
Access Cavity Perforation - Types of Root Perforation Can occur during access cavity perforation (specially in anterior) Due to misdirection of bur during access cavit
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