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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is the mode of nutrition in fish,human,amoeba,scorpian & toad ?
How to monitor body weight to enhance athletic performance? Monitoring body weight is a practical way to assess energy balance. Weight stability, particularly during periods
a) List the a variety of modes of heterotrophic nutrition in plants. b) Define any two modes giving one example of each.
Determine the appearance of a plant cell when water is plentiful? When water is plentiful, the central vacuole expands. The other organelles are pushed against the plasma me
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