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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is the difference between taeniasis and cysticercosis? The Taeniasis is the parasitic disease caused by the adult tapeworm installed within the human intestine. The
Ethylene glycol is a poison that causes about fifty deaths a year in the United States. Treating people who have drunk ethylene glycol with massive doses of ethanol can save their
Does every gene mutation cause alteration in the protein the gene normally codifies? Not each gene mutation causes alteration in the composition of the protein the gene codifie
Of what importance are lactic acid fermentation and alcoholic fermentation to the cells that use these pathways? These pathways regenerate NAD, which the cells can use to keep
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Where do the two neuronal pathways diverge For both the neuronal pathways i.e. light reflex and near reflex the efferent fibres go to are 3rd cranial nerve and then to sphinct
Illustrate in detail about the Cell theory All living organisms are composed of cells, and they are the basic structural and functional units of life, just like the atom is a
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