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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Annuloplasty with a Ring : The lings commonly used are Carpentier- Edwards and Cosgrove's flexible half ring. The rings are C shaped and have incomplete circles to accommoda
For homework, I had to prove that a certain catalyst was a protein, then tell how I would purify the protein, then tell how I would clone a human chromosomal region that contains t
Advice on Discharge : Patients are allowed to go home on 8th or 9th day. It could be even earlier after off pump coronary artery surgery (OPCAB). They should gradually increase t
Explain about the Phosphorus - Macro Minerals? Phosphorus is the second most abundant element in the human body, comprising 30% of the total mineral content. An adult human bod
Explain the Burden of Hypertension in heart diseases ? Since 1942, there have been several small and large population-based studies on Hypertension. A ineta-analysis showed an
Q.Explain Pericardial Effusion? The configuration of the heart in pericardial effusion depends on the volume of fluid and its distribution. It may have a globular or non-speci
conductometry and its uses in biological experiments
negative and positive feedback
Q. Illustrate Wild Life Sanctuaries? You must have seen how some of the parks are fenced. The intention is to keep the animals from wandering out and not to destroy neighbourin
Q. Why is the occurrence of eyelids in amphibians in comparison to their absence in fishes an adaptation to terrestrial life? Eyelids associated to lacrimal glands protect and
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