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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is population density? The Population density is the relation between the number of individuals of a population and the area or volume they occupy. For instance, in 200
METABOLISM - Word given by Shwann . It includes bio-chemical reactions occur in body may be constructive or destructive. It includes - Anabo
Assessment 1. Assess the past and present history of illness, sore throat, rheumatic fever and treatment received previously. 2. Family history of rheumatic fever. 3. S
Explain the Food Applications of Gum Arabic A major use for gum Arabic is in the confectionery industry where it has two important functions; to retard or to prevent crysta
outline the process of yeast fermentation stating all enzymes involved
(i) Effect on Human Health: Air pollution causes many respiratory problems in human being. (1) Dust, soot and smog causes several respiratory troubles such as bronc
Define Procedure for estimation of vitamin c - colorimetric method? Carry out the experiment following the instructions given herewith: 1. Prepare a series of tubes for blan
Food Applications of hemicelluloses The hemicelluloses find their application in food systems as emulsifer, stabilizer and binder in flavor bases, dressings and pudding mixes.
nervous system structure
Q. Physiological Characteristics of molds? The physiological characteristics of molds will be reviewed only briefly here and will be discussed in more detail subsequently. Mois
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