Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Pasteur''s experiment, Louis Pasteur (1860-1862) French): Even after th...

Louis Pasteur (1860-1862) French): Even after the experiments of Spallanzani, the conflict of a biogenesis and biogenesis continued well into the middle of 19 th century till

Fertilization, Fertilization is the fusion of the two gametes (sperm and o...

Fertilization is the fusion of the two gametes (sperm and ovum) to produce a zygote which develops into the new individual with a genetic heritage derived from both the parents. S

What is sick sinus syndrome, Q. What is Sick Sinus Syndrome? Ans. ...

Q. What is Sick Sinus Syndrome? Ans. When a slow resting pulse fails to accelerate normally with exercise, it has been labeled chronotropic incompetence, however, there i

What are the applications of pcr, Polymerase chain reaction already has ext...

Polymerase chain reaction already has extremely widespread applications and new uses are being devised on a regular basis. Some and definitely not all of the applications are given

Cloning protocol, Sally Subcloner wants to clone a fragment into pUC19 (Map...

Sally Subcloner wants to clone a fragment into pUC19 (Map above – From New England Biolabs). Her fragment was a protein coding gene of 2.7 kb, and had XhoI sites on either end (and

Preparing financial statements from the work sheet, When the work sheet is ...

When the work sheet is finished all the essential information to prepare the statement of retained earnings, income statement and balance sheet is readily available. Currently you

State about the latent squint disease, State about the Latent squint diseas...

State about the Latent squint disease Latent squint is seen commonly as exophoria in infancy and presbyopic age and esophoria in childhood. Hypermetropes can have esophoria and

Explain the term - diffusion, Explain the term-Diffusion Diffusion occu...

Explain the term-Diffusion Diffusion occurs from the side of greater concentration to the side of lesser concentration. In aqueous humour this occurs from the plasma side in th

Role of lymphatic system - circulation, Normal 0 false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Research and documentation - conservation of wildlife, Research and Documen...

Research and Documentation - Conservation of wildlife First, list of endangered species are established by various national and international agencies. Another important actio

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd