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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Autotrophic and Heterotrophic Succession The succession where initially the green plants are much greater in quantity than the animals, is known as autotrophic succession. Su
What is the role of Linked Traits in genetics? In his study of inheritance in the fruit fly, Morgan observed that certain genes are distributed together, or linked, rather than
Based on your reading of the article entitled "The Evolution of Color Vision", which of the following is a false statement? A. Trichomatic vision is found in all male and femal
Q. What is Colony Collapse Disorder? Bee colonies are attacked by a variety of pathogens and parasites. One major pathogen is the acute bee paralysis virus (IAPV), which cau
What is Machined implant Machined implant has irregular surface roughness from approximately 10μm grooves and ridges in the machining direction. The roughness of implants can
Define some Precautions for Estimation of the Amount of Bacteria? 1. Dilution should be made carefully. 2. Use fresh sterile pipette for making each dilution. 3. Aseptic
Use of trichomoniasis in pregnancy Trichomoniasis in pregnancy has been associated with adverse pregnancy outcomes (D Soper, Am J Obstet Gynecol 2004; 190:281). Metronidazole
The Eukaryotic transcription is more difficult than prokaryotic transcription. For example, in eukaryotes the genetic material DNA and therefore transcription is basically localize
Q. Concerning tissue complexity how dissimilar are cnidarians from poriferans? Poriferans present only some dispersed specialized cells with no tissue differentiation, Cnidaria
Explain a review of Eating Disorder? If you talk to a group of young boys and girls in an informal setting about their physical appearance, you will find that a majority of the
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