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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Traumatology : This is the study of wounds. In other words we can say that traumatology is the study of wounds or injuries which can be caused by accidents or violence to a person
What is cytoskeleton? What are its main constituents in animal cells? Ans) Cytoskeleton is the cytoplasmic structure that handles the cell, keeps its shape and fixates and moves
A simple method used to identify areas of high conservation priority is the selection of countries according to highest species richness. Among the top 12 countries identified this
What is the normal duration of the menstrual cycle? How does the calendar contraceptive method work? The normal duration of the menstrual cycle is 28 days but it can vary betwe
Which type of cell came first in evolution the eukaryotic cell or the prokaryotic cell? Ans) This is an interesting problem of biological evolution. The most accepted hypothesis
what is the correct classification of phylum mollasca
In a comparison of brain found in lower classes of vertebrates to the brain in higher classes of vertebrate's area showing most increase in relative size is: a) Optic lobe b
Stress - Responses of Plants to Stress Let us recall what happens within the natural communities occupying the same habitat. The relative location of two plants may place them
Are nematodes diploblastic or triploblastic animals? Just like platyhelminthes, nematodes are triploblastics, i.e., they show three germ layers (ectoderm, mesoderm and endoder
What is Simple Transposition in Neonates ? A baby with this malformation needs to be operated without delay. A very cyanosed infant will require palliation by balloon arteries
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