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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
#question.what is the mechanism of circulatory system .
Write a note on cutaneous respiration in frog
Define Proteins required for underweight - Nutritional Care? Proteins are required for tissue building, as well as, to take care of the daily wear and tear. Under weight indivi
Why roots of many swamp plants have a special morphology? The Swamp and The marsh plants in general present supporting roots that ramify from portions of the stem above the gro
In a cohort study that found an association between alcohol consumption and bladder cancer, 20,000 middle-aged men were asked about their drinking habits and then tracked for five
Explain the Absorption and Transport of Vitamin E? Absorption of vitamin E from the intestine depends on adequate pancreatic function, biliary secretion and micelle form
WHAT IS PANDA BAO BAOLOGY.?
Into which classes are mollusc divided? What are some representing beings of each class? The phylum Mollusca is separated into five major classes: pelecypods, or bivalves (Pele
Liver necrosis?
Of which type of defense cell do worm infections stimulate the multiplication? The major leukocytes that generally multiply and participate in the defense against worm infectio
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