Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Can you explain non-restrictive ventricular septal defect, Q. Can you expla...

Q. Can you explain Non-restrictive ventricular septal defect? In non-restrictive VSD PA pressures are elevated hence VSD gradient is low. Non-restrictive VSD is larger in s

Define sex-linked inheritance of nonmendelian inheritance, Why is sex-linke...

Why is sex-linked inheritance an example of nonmendelian inheritance? The Sex-linked inheritance is a kind of nonmendelian inheritance because it opposes Mendel's first law, wh

Determine the effect of iodine deficiency, Determine the Effect of Iodine D...

Determine the Effect of Iodine Deficiency? Iodine deficiency affects all populations at all stages of life, from the intrauterine stage to old age. However, pregnant women, lac

Vesicular exanthema, Vesicular exanthema Vesicular exanthema of pigs close...

Vesicular exanthema Vesicular exanthema of pigs closely resembles foot-and-mouth disease in these animals. The virus causing the disease belongs to the genus Calicivirus in the fa

Craninal nerves, Craninal nerves: Cranial nerves take their origin f...

Craninal nerves: Cranial nerves take their origin from different areas of brain  Some of them are sensory, some are motor and a few are mixed nerves. There are 12 pair

Explain acid and bases, Explain acid and bases? Acids and Bases :  So...

Explain acid and bases? Acids and Bases :  Some aqueous solutions, however, such as HCl, form an excess of hydrogen ions [ H + ] and are called acids. Others, such as NaOH,

Biochemistry, Water is most dense and thus heavier at 4 degreese celceus. A...

Water is most dense and thus heavier at 4 degreese celceus. At 0 degreese celceus ice forms and can float on liquid water. Assuming that ice were most dense at 0 degreese celceus,

#title.circulatory system, #question.what is the mechanism of circulatory s...

#question.what is the mechanism of circulatory system .

Important conditions for malabsorption syndrome, Q. Important conditions fo...

Q. Important conditions for malabsorption syndrome? Let us now discuss a few important conditions grouped collectively under the term of malabsorption syndrome. These are:

How can you categorize flowering plants, Q. What are the two major groups i...

Q. What are the two major groups into which flowering plants are divided? Angiosperm plants are separated into monocotyledonous (monocots) and dicotyledonous (dicots).

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd