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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is mucoperiosteal flap Silk is easy to handle, ties with a slip knot, and is relatively inexpensive compared with other nonabsorbable suture materials currently available.
Synthesis of citratefrom acetyl CoA and oxaloacetate: Citrate synthase catalyses this aldol condensation reaction with the release of CoA. There are certain inhibitors to this re
economic importance
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What is the function of the feet in molluscs? How is the mollusc foot related to the name given to the classes of the phylum? The mollusc foot has the function of locomotion, s
In the living condition (found in sea) you would be able to observe the following features in radiolarians: The cytoplasm is divided by a perforated and chitinous metnbrane into
Explain briefly why 'greenhouse' gases lead to global warming. The greenhouse gases do not interfere with the short-wave radiation reaching the Earth from the sun but take up t
Solar Tracking - Nastic and Epinastic Responses Many plants such as sun flowers are capable of solar tracking in which the flat blade of leaves or inflorescence will remain a
What Do We Mean By Culture Media? Culture media, you would realize, is a solid or liquid preparation containing all the nutrients required by microbes for growth. So then, what
Limiting Factor - Ecosystem In all ecosystems one factor, usually abiotic, limits the growth of organisms and is therefore called a limiting factor. The limiting factor is one
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