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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Types of Forests - Ecosystem The forest biomes of the world have been classified into the three major categories: coniferous forest, tropical forest and temperate forest. C
Respiratory Gases -Respiration Physiologically the most important gases are oxygen, carbon dioxide and nitrogen. Molecular oxygen comprises 21% of the atmosphere; carbon diox
Which of the following is NOT produced by the hypothalamus? A) releasing hormones B) inhibiting hormones C) antidiuretic hormone D) oxytocin E) thyroid-stimulating h
how mollusca bore into hard substrate
HOW ARE THE ORIGIN AND EVOLUTION OF ALGAE..
Q. Fats requirement in chronic diarrhoea? Total amount of fat may be restricted as its digestion and absorption is compromised. In order to increase on the calorie density of t
What is the compound that is phosphorylated for ATP formation? What is the resulting compound when ATP liberates energy? ATP, or adenosine triphosphate, is produced after the
What is normal bone physiology An insight into the normal bone physiology and its adaptation by modeling and remodelling process to functional stresses is also of importance. I
Q. Illustrate Pathology? Following rheumatic fever, over next few years to decades, the typical funnel shaped mitral valve assumes a fish mouth appearance. During rheumatic fev
Aeromonas associated zoonotic disease Aeromonas causes gastrointestinal infections and extra intestinal infections such as cellulitis, wound infectiopn, peritonitis, endocardi
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