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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Besides being fundamental for the activation of the main gastric digestive enzyme how does HCl also directly participate in digestion? With its corrosive effect HCl also hel
Influenza Influenza is an acute infectious disease caused by influenza viruses of genus Orthomyxovirus in family Orthomyxoviridae. The name Influenza is derived from an Italian ph
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Tracheae - Respiratory Organs Tracheal respiration is characteristic of insects. Onychophorans, diplopods, arachnids, and chilopods as well use tracheal system for respiration
What are the major functions of the organic molecules for living beings? Organic molecules, such as proteins, lipids and carbohydrates, perform numerous functions for living or
Explain Objectives of Root-End Resection Apicoectomy a) Removal of untreated apical portion of the root, b) Enable the obturator to determine the cause of failure, c) To
Q. Explain about Gastro Oesophageal Reflux Disease? GERD refers to the regurgitation of acidic stomach contents into the oesophagus. It results in a spectrum of clinical manife
Enzyme Activity Enzymes are biomolecules which catalyze (i.e. increase the rates of) chemical reactions. Almost all enzymes are proteins. In enzymatic reactions, the molecules
Define Methods of Food Processing? Food is undeniably most vital to the survival of human beings. Hence, it must be processed using various scientific techniques. This is done
What proportion of children with Down syndrome do you expect when women with down syndrome have children with men who have 46 chromosomes? Justify answer
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