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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are some human diseases caused by virus and what are their respective modes of transmission? The main viral diseases transmitted by respiratory secretions (cough, sneezes)
Explain about Stacking faults Stacking faults happen in a number of crystal structures, but the common example is in close-packed structures. Face-centered cubic (fcc) struct
Biodiversity maintains the air we breathe and the water we drink. Green plants purify our air and our water by taking in carbon dioxide, regulating water vapour, releasing oxygen,
Littoral and Benthic Zone Littoral Zone This is the shallow water zone, near the shore, where light penetrates to the bottom. Rooted plants can grow only in this region.
Explain Acyclovir Available in topical, oral, and intravenous (IV) formulations, acyclovir is used to treat herpes simplex virus (HSV) and varicella-zoster virus (VZV) infectio
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The calorific value of a fuel can be theoretically determined by using dulong's formula. It is assumed that heat evolved comes from the combustion of carbon, hydrogen, and sulphur
What type of bonds is susceptible to hydrolysis? Book examples are C-N and C-OH.
Explain the Glass Test Tubes - Food Microbiology Sterile glass tubes are used for culturing microorganisms in liquid or solid media. Sterility in tubes is maintained by using c
Q. What is the relation between the concepts of chromosome and chromatin? Are heterochromatin and euchromatin part of chromosomes? Every filament of chromatin is a complete DNA
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