Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Circulatory system, Circulatio n I n  Animal Circulation is served ...

Circulatio n I n  Animal Circulation is served by a fluid as blood/ lymph that circulates through the body in a regular manner, receiving & distributing materials in the wa

Define half saturation test and full saturation test, Define Half Saturatio...

Define Half Saturation Test and Full Saturation Test? This test is specific to polysaccharides. The test is used to detect dextrin from starch. The details related to the test

Main species of taenias that cause human diseases, Q. What are the two main...

Q. What are the two main species of taenias that cause human diseases? The two chief species of taenias that cause disease in humans are Taenia solium, or the pork tapeworm, an

Find a chemically gated protein, You have identified a protein in a cell th...

You have identified a protein in a cell that you know that comes from the human body. With respect to that protein you have also determined that it allows sodium ions to go through

Vitamins, Vitamins Animals cannot sustain a healthy life if they are ...

Vitamins Animals cannot sustain a healthy life if they are fed on a diet having only carbohydrates, fats and proteins. They also require vitamins in small quantities in the r

Why the two dna polymerase proteins are oriented in opposite, Which of the ...

Which of the following best defines why the two DNA polymerase proteins that are held by the sliding clamp are oriented in opposite directions? A. The efficiency of replication

What are the common changes in the eye, What are the common changes in the ...

What are the common changes in the eye Other changes in the eye are: 1) Increase in curvature of anterior surface of lens. 2) Anterior pole of lens moves forwards without

What is fern allies - equisetum, What is Fern Allies - Equisetum ? The ...

What is Fern Allies - Equisetum ? The earliest land plants are thought to have evolved from green algal ancestors, since they share many features. Both green algae and plants h

What is the procedure of examining foot, Procedure of Examining Foot Le...

Procedure of Examining Foot Let us learn the steps of examining the foot and identify foot at risk. Steps to follow: 1. Make the patient comfortable. 2. Provide priv

Bacillary dysentery, Bacillary Dysentery: You have learnt about the di...

Bacillary Dysentery: You have learnt about the diarrhoea in the foregoing sub-section, now let us take for example a child who has loose motion which contains blood and mucus

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd