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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is the difference between metabolic acidosis and respiratory acidosis and what is the difference between metabolic alkalosis and respiratory alkalosis? Respiratory acid
Metamorphosis The phenomenon of metamorphosis is described as a process during development which involves a remarkable change in morphology and physiology of the larva, so tha
Explain Factors influencing food production? Appropriate food production involves getting an adequate output (i.e. food) using appropriate inputs. An essential requisite for th
To develop the food exchange list, a doctor or dietician keeps in mind four important basic points. These are: 1) Group similar foods in one group. For example cereals, roots,
The five kingdom systems To cope up with above discussed problem a number of alternative classificatory schemes have been suggested with more than two kingdoms. The one which h
Benzathine penicillin given every three weeks as IM injection (1.2 mega units) has given the best results. Oral agents (penicillin V) are given in patients with lower risk of rheum
Q. What do you mean by Recovery Period? At the instant exercise is discontinued, the ECG recorded is turned on and left running for a few seconds while the blood pressure is re
what is mean by fisson
information on climbing beans
Q. What are the five classes of vertebrates? To which of these do human beings belong? The five classes of vertebrates are amphibians, reptiles, fishes (osteichthyes and chondr
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