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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is the cyclosporin? How are fungi related to this substance? Cyclosporin is a drug discovered in the 1970's that revolutionized organ transplantation in Medicine, It is
What is ancylostomiasis? Ancylostomiasis is a disease caused by Ancylostoma duodenale or Necator americanus, both hookworms belonging to the nematode phylum (roundworms). Ancyl
Describe Gastrovascular cavity in detail? The name given to the internal cavity of cnidarians. A blind-ended (incomplete) gut with only one opening. Food is to be digested and
Define Factors Affecting Energy Expenditure and Requirement? As mentioned earlier, the energy needs vary widely among individuals in a group. Why? A number of factors cause the
Active Transport We have mentioned above that Transmembrane potential develops due to active transport of ions (H + ) outside the cell. Since this transport takes place again&
Describe cellular location and the basic processes of photosynthesis.
How do you draw in organic chemistry format a carbon double bonded to an oxygen atom when this carbon is the first carbon on a carbon skeleton of a molecule?
Regulation of Glycolysis There are three markedly exergonic reactions in the glycolytic pathway, which are considered physiologically irreversible. These reactions are cataly
Blood from a donor is sterile and stored in a sealed bag, but it is still kept at 4°C. What is the advantage of keeping it at this low temperature? At 4 °C, enzyme activi
Why are agricultural fields often less stable than natural communities in the similar area? Because agricultural fields usually having one species of crop plant, they have low
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