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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain about the various Cooking methods? All of us eat food either raw or in cooked form. Have you ever thought why we need to cook food? Cooking is a primary process to mak
Explain the difference between meiosos and mitosis
What is Arsenite inhibitor Arsenite inhibits synthesis of ATP, in the conversion of 1,3-bisphosphoglycerate to 3- phosphoglycerate, by causing uncoupling of oxidation and
What are Herbicide tolerant crops in ecological Herbicide tolerant crops (HTC) Ecological a) Increased use of the herbicides that plant is tolerant to (describes ecologi
Tikka Disease in Groundnut
How different are reptiles and birds concerning the maintenance of body temperature? Are birds rare in polar regions? Reptiles are heterothermic, i.e., they do not control thei
Question 1 What is autologous transfusion? Discuss how would you handle and prepare blood for autologous blood transfusion. List the advantages of autologous transfusion Qu
Captive Breeding - Measures for Species Conservation Species which are reduced to dangerous levels need more intensive management, and one strategy is their captive breeding.
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Q. Explain about Gout disease? Gout is a chronic disease due to an inherited abnormality of purine metabolism. What are purines? You may recall studying about purines and their
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