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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Miscellaneous Functions of Protein? Besides the functions enumerated above certain other important miscellaneous functions of proteins are included herewith. These inclu
In the long term reversible causes of heart failure like valvular lesions, myocardial ischemia, uncontrolled hypertension, arrhythmias, alcohol, negative inotropic agents, intracar
What is Fern Allies - Equisetum ? The earliest land plants are thought to have evolved from green algal ancestors, since they share many features. Both green algae and plants h
Fructose is an abundant sugar in the human diet; sucrose (table sugar) is a disaccharide that when hydrolyzed yields glucose and fructose and fructose is also a major sugar in hone
Q. What are the morphological differences and similarities between nematodes and annelids? Nematodes, like annelids, have a cylindrical elongated body. Annelids differentiate f
Hereditary fructose interolance is a rare recessive disorder that is caused by a mutation in the aldolase B gene. As a result of this disorder individuals may have all of the follo
Q. Etiological factors contributing to lactose intolerance? The etiological factors contributing to lactose intolerance include: • Genetic factor • Reduction in jejunal
Define Excretion of toxins and Act as precursors? Excretion of toxins: Glucuronic acid, a metabolite of glucose, combines with chemical and bacterial toxins and some normal met
Q. Show How Adaptation affecting taste quality? Adaptation - A low concentration solution will not give any sensation after tasting a higher concentration solution due to the a
List the four basic points to be kept in mind when advising food exchange. To develop the food exchange list points to keep in mind are: a) Group similar foods in one group.
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