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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
ISOZYMES Sometimes an enzyme present in the same organism is found to have different molecular forms but catalyzing the same reaction. These are called isozymes. Th
heating a food sample with Benedict''s solution is a test for...
Define Growth and Development of Infants and Preschoolers? In this unit, we learnt about the various aspects related to the growth and development of infants and preschoolers.
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summarise the comparative anatomy of the digestive system of the invertebrate and the vertebrate.
how can i read role od fungi as saprophyte?
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