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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Explain Treatment of Acute pericarditis? Symptomatic Treatment Pain relief can be achieved with indomethacin 25 to 50 mg. thrice daily; Ibuprofen 600 mg. thrice daily
Weaning the Infant from the Incubator When heater output reading is minimal or nil it suggest that infant is capable of generating enough metabolic heat to keep himself war
Arteries - Circulation The arteries deliver blood from the heart. Figure shows the structure of arteries and the different layers of the vessel wall. The thick walls of these
Q. What are pericardium, pleura and peritoneum? Pleura are the membrane that covers the inner wall and the lungs of the chest pericardium are the membrane that covers the heart
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Q. Medical and Nutritional Management for pectic ulcer? To provide physiological rest and support tissue healing, treatment should be based on providing rest to the affected ar
Explain Taxonomic Concepts and Their Development? The history of classification is an exciting aspect of plant taxonomy. The discovery of the use of plants for food and later
Method for Radiographic evaluation of the outcome or RCT Ørstavik and associates suggest the use of the periapical index (PAI) for radiographic evaluation of the outcome of roo
DISORDER OF SKELETON AND JOINTS - 1 . Sprain - It involves a stretching or tearing of tendons or ligaments. 2. Arthritis - It is infalmation of joints.
Shift of the phosphate group from carbon 3 to carbon 2 Shift of thephosphate group form carbon 3 to carbon 2: This reversible reaction is catalyzed by phosphoglycerate mutas
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