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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define the effect of Dietary fibre on Satiety? Satiety: Several investigators have speculated that ingestion of a high fibre food induces a feeling of satiety, reduces meal siz
Measures of Evenness The maximum Shannon-Wiener index for a given number of species can be calculated as: The minimum Shannon-Wiener index for a given data set can be c
Palynofossil s (Microfossils) - Fossils of planktons, diatoms, unicellular algae (diatoms) and shelled protozoans. (Heliozoans, Radiolarian and Foraminifera). Its finding in s
economic importance of tubularia
In the scientific competition against fixism what are the main arguments that favor evolutionism? The major arguments in favor of evolutionism are: paleontological, from the st
Q. What is the mitosis? What is the significance of mitosis? Mitosis is the process in which one eukaryotic cell divides into two cells identical to the parent cell generally i
Explain Corrective Surgery - Endodontic Surgery perforation repair periodontal management (root resection, tooth resection) intentional reimplantation
Nutrient Cycling in Tropical and Temperate Forests From this study of the nutrient cycles you must have realised the importance of the role of green plants that take up nutri
Q. Dietary approach for pectic ulcer? Let us now move on to understand the dietary approach. We shall begin with the nutrient requirements of the patients. However before we ta
what is hydrostatic skeleton?
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