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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Descent of the Testis The testis develops on the posterior abdominal wall at the mesonephric ridge. To reach the adult position in the scrotum, it must decent. A fibrous cor
What is Respiratory System explain their functionality ? The respiratory system is responsible for the transport of oxygen and carbon dioxide from outside the body to and from
Determine the term - Neuropsychological approach The neuropsychological approach relies on the use of tests in which poor performance may indicate either focal (localised) or d
Please consider the phenomenon of bacterial resistance to antibiotics. How did the combination of human advances and basic evolutionary principles lead to this situation? How is it
how to determine that the phenotype of a wild type is due to the action of one or two genes?
Define Solubility affecting absorption of dietary iron? Solubility is crucial for non-haem iron absorption as the inorganic iron salts have to be solubilized in the intestine f
Define Nutritional Requirements of Fats and Oils during Pregnancy and Lactation? ICMR (1990) recommends that linoleic acid requirements should be raised to 4.5 en% during pregn
Acute Rheumatic Fever Rheumatic fever is an inflammatory disease that usually follows infection of upper respiratory tract with group A beta hemolytic streptococci. It is c
SOM E COMMON REFLEXES RELATED TO RESPIRATION - 1. Cough Reflex: Due to stimulation in Trachea before it 2.5 lit. air is inhaled. 2. Sneezing Reflex: Stimulati
What is the vector of Chagas' disease? How is the disease transmitted? The vector of Chagas' disease is its middle host, a triatomine bug. The major species is Triatoma infesta
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