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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
explain the modes of nurition in animals
Describe in detail about the chlorophyll molecule Each chlorophyll molecule contains magnesium ion, there could be no green plants without magnesium. As magnesium is present in
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Humans produce insulin from certain cells in the pancreas.The insulin gene is isolated from a human pancreas cell and then inserted into a plasmid.The DNA responsible for the synth
How Viruses Multiply? Obligatory parasitism - Outside cells viruses are nonliving, inactive particles but after entering into live cells these multiply fast by replication
What gases will be taken in and given out by a green plant (a) in darkness, (b) in bright sunlight? a) In low light intensities a green plant will be taking in oxygen and
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