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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Does thermal inversion occur in the winter or in the summer? The Pollutant low altitude thermal inversion take place in the winter and in this period of the year the sun hea
Q What is the radula? What is the function of this organ? Some molluscs have a tongue-like structure with harsh saliences similar to small teeth. This structure is called as ra
Diabetes Mellitus Management after Open Heart Surgery : Diabetic patients undergoing coronary artery bypass surgery who are on oral hypoglycemic drugs are pre operatively s
Lipid profile control Evaluation of lipid profile is essential to the management of diabetes and should be performed at initial visit in all patients with diabetes. You have l
Q. Is the epithelium vascularized? How do oxygen and nutrients reach the epithelium? Why is this feature an important evolutionary acquisition? Epithelia are not vascularized c
Oncogene is the gene in a tumor virus or in the cancerous cells which, when transferred into the other cells, can cause the transformation (note which only certain cells are susce
What are the main types of waste? The waste can be classified into main types, each of them carrying its own dissimilar environmental problem: organic waste, non recyclable was
Mention causes of implant failure due to improper implant selection. Implant selection errors : a) Improper implant type in improper bone type. b) Improper length, width
Candidiasis Candidiasis (Moniliasis) is a disease of man, animals and birds and is caused chiefly by Candida albicans. Crop mycosis (thrush) of poultry is caused by C. albicans
What is the difference between the concepts of karyotype and genome? Genome is the set of DNA molecules that characterizes each living being or every species. The concept then
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