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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Regents for Estimation of Vitamin C - Colorimetric Method? 1. 2, 4 dinitrophenylhydrazine reagent - Dissolve 2 g of dinitrophenyl hydrazine in 100 ml of 9N sulphuric aci
natural selection
Rejection Reaction For rejection reaction the physiological and biochemical processes are set in the pistil by the recognition reaction specific to the type of pollen that lan
In the last unit we traced the human evolutionary history mostly from a palaeontological viewpoint. Although still fragmentary, the available fossil evidence makes it possible to c
what is virus
Cilia and flagella share a common structure, with a microtubular core which has a 9+2 organization with nine paired doublets of microtubules surrounding a central pair to form the
harmful and beneficial usesa of phylum porifera
Cancer ; Cell division , like all other biological processes, is under genetic control. Certain genes must regulate the processes of cell growth and division in respons
The transducer is placed in the mid axillary line with the transducer ridge pointing towards the patients left to obtain the four-chamber view. The left ventricular apex is
what is cromosome jumping
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