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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Regulation of Ammonia Assimilation Heterotrophic bacteria like Escherichia coli and Klebsiella aerogenes induce the operation of GS-glutamate synthase pathway of ammonia assim
Define the Buffer capacity - Nutritional Biochemistry? Buffer capacity can be defined as, 'the capacity of a solution to resist changes in pH on the addition of strong acid or
Heart of cockroach: In cockroach, heart is situated in pericardial sinus. It has13 chambers These are one behind the other. Each chamber opens into preceeding one. The
Defence mechanisms of the host The healthy animal is protected from pathogenic organisms present in the environment by virtue of the effective immune mechanism present from bi
How does phosphocreatine act in the muscle contraction and relaxation? Phosphocreatine is the main means of energy storage of the muscle cells. During relaxed periods ATP mo
Acetylcholine, a well known neurotransmitter, plays a critical synaptic role in the initial formation of memory. Chemical blockage of the acetylcholine receptors makes it harder to
Q. Bird identity card. How are birds characterized according to examples of representing beings, basic morphology, respiration, skin, nitrogen waste, circulation,thermal control an
Define Stage - Parts of the Microscope? It is a fixed platform positioned about halfway up the arm and provide surface for keeping the slide. Slide is held in position either b
VAS A DIFFRENTIA - Originate from wollfian duct . It comes out fo cauda epdidymis enters into abdominal cavity through inguinal canal, forms loop round the uretor and j
Which of the following pairs of events in a human female occur at, or nearly at, the similar time during the menstrual cycle? A. High blood plasma LH levels and ovulation. B
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