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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
why obelia shows an intermediate grade of organisation
Wastes are classified into following categories: 1. Biodegradable wastes: The waste materials that can be degraded by micro-organisms are called biodegradable wastes. Th
Preoperative antibiotics Preoperative antibiotics can decrease the incidence of infection after colorectal surgery; for elective operations, an oral regimen of neomycin (not a
Q. What is meant by unsaturation or saturation of oils and fats? When it is said that a triglyceride is saturated it means that in its molecule the carbon chain is bound in its
What are the parasympathetic neurons Patient A has a disease that has destroyed half of the alpha-adrenergic receptors on each of the smooth muscles that control the diameter o
Direct demonstration of the causative agent by electron microscopy (EM): Where facilities for electron microscopy are available and a viral disease is suspected, presence o
Insulin Devices - Insulin Pen Insulin pen is an insulin injection system. A pen consists of disposable needle, a vial of insulin and a pen. There are two pen systems replac
Extended point within pulp chamber: Gripping action: - Steglitz forceps or pin pilers. - Access cavity flooded with chloroform to dissolve the sealer. - Grasp the
Explain Trifluridine (Viroptic) Trifluridine is a nucleoside analog active against herpes viruses, including acyclovir-resistant strains. Marketed in an ophthalmic preparatio
Benefits of Computerization Computerization were the following benefits in nursing: Improved professional care Better accuracy (by decreasing human error) Effect
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