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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are Cytokines? Cytokines are polypeptides, which influence the proliferation, differentiation, metabolism and activation of cells. There are three areas in cancer that hav
Q. What are the main opportunistic diseases that can affect AIDS patients? Among the opportunistic diseases that affect HIV contaminated people during the AIDS stage some are:
diagram of dna replication
Root - Plant Water Relation Root system is directly related to the absorption of water and its growth under field conditions is very much influenced by soil. In dry land agric
Q. What are some antibiotics used against fungi? The systemic or topical azoles (like fluconazole, itraconazole and others), amphotericin B, the echinocandins (caspofungin, mic
What is the difference between a hormone and a morphogen? How do they act and what type of development do they control?
Aeromonas associated zoonotic disease Aeromonas causes gastrointestinal infections and extra intestinal infections such as cellulitis, wound infectiopn, peritonitis, endocardi
Q. What are the major events of the first mitotic period? The first mitotic period is the prophase. During prophase the following events occur migration of each centriole pair
Explain the Probiotics in Foods? Yoghurts have been supplemented with probiotic strains of bifidobacteria and Lactobacilli. Milks fermented solely by intestinal isolates of lac
Explain water Universal Solvent ? Water - Universal Solvent : Water is usually referred to as the "universal solvent." because it is able to dissolve many different substan
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