Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What is hemoglobin s, Question 1 What is megalobastic anemia? Discuss brie...

Question 1 What is megalobastic anemia? Discuss briefly its lab diagnosis. How would you differentiate megaloblastic anemia from other anemias? Question 2 What is hemog

What do you meant by medical writing, Question 1 : What do you meant by ...

Question 1 : What do you meant by medical writing? Show various types of medical writing. Define and explain briefly medical writing Discuss various types of medical w

Brasseler endo extractor tube-endodontics principles, Brasseler Endo Extrac...

Brasseler Endo Extractor tube Insert H file buccally and lingually and remove silver by friction.If I can't remove use solvent to desolve cement If the tip of silver not appear

Define tests to measure flexibility component in the humans, Define Tests t...

Define Tests to Measure Flexibility Component in the Humans? The flexibility component measures the elasticity or range of motion around a joint. This component of physical fi

Pollen biology, Pollen Biology Fluorescence microscopy coupled with bi...

Pollen Biology Fluorescence microscopy coupled with biochemical analysis has helped resolve the differential activity of the enzyme, β-l, 3- glucanase that catalyses the disso

What do you understand by chromosome behaviour, Q. What do you understand b...

Q. What do you understand by Chromosome Behaviour? When we study meiosis. we not only observe the regularity of pairing which is important' for' the fertility of the plants, we

Anterior leaflet procedure-technique of operation, Anterior Leaflet Procedu...

Anterior Leaflet Procedure: Repair is more difficult and less successful. Previously, a triangular excision of the anterior leaflet used to be done for prolapse and rupture of

Determine the chance of developing cancer, A typical chest x-ray exposes th...

A typical chest x-ray exposes the patient to a radiation dose of 0.01 rem. If a man receives 10 such x-rays in a lifetime, what is his chance of developing cancer as a result of th

Natality rate - natality, Natality Rate - Natality Natality rate or bi...

Natality Rate - Natality Natality rate or birth rate is determined by dividing the number of individuals born by unit time and is expressed as follows: Natality rate =  ΔNn

Germinative zone , what is the function of the germinative zone

what is the function of the germinative zone

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd