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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Production Efficiency - Ecosystem The maximum amount of solar energy harvested by plants is about 5 per cent but the average for green plants, on the whole is only a small fra
Define the term - lamellac The inner membrane is folded into very closely packed membranes called the lamellac. The lamellae flatten out at regular intervals into structures c
An element playing significant role in nitrogen fixation is : 1. Molybdenum 2. Copper 3. Manganese 4. Zinc An element playing significant role in nitrogen fixation
List the five major phases of the cell cycle, and briefly explain what happens during each phase. G1 phase: the cell grows. S phase: DNA is copied. G2 phase: the cell prepares
How to reduce calorie intake from fat? Efforts should be made to increase the nutrition knowledge of the general public through mass media. The foods with lower fat co
Demonstration of specific antigen(s): The presence of the viral antigen in the infected tissues and cells can be demonstrated by fluorescent antibody technique (FAT) and
Explain the Peptones - Complex Media? Peptones are protein hydrolysates obtained by partial digestion of meat, casein, soya meal, gelatin or other protein source. These provide
Subphylum Mandibulata Most of them have three pairs of walking legs, mandibles, compound eyes, antennas, some with wings. There are four classes. Crustacea - aquatic with
Explain Monascus species Most of the reports of food colourants from microbial sources involve Monascus species, particularly Monascus purpureus, followed by Rhodotorula speci
Please help with the following: a) Draw a diagram (showing all the C and H atoms, and their bonds) for a hydrocarbon with 5 carbons with a double bond between carbons 2 and 3.
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