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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is the difference among cryptogamic and phanerogamic plants? Cryptogamic (hidden sex organs) plants are those that do not show flowers or seeds. They comprise the bryophy
What are some ways that humans can reduce the effect they have on the phosphorus cycle?
S-Gene The S-gene has been suggested to be a super gene complex with several linked genes. It is supposed to have at least six (may he more) closely-linked genes which determi
Facilitation Model - Models of Succession This is considered as the classical model of succession. It is based on the assumption that species of a previous stage are replaced
Q. What are the digestive functions of the liver? The liver has other digestive functions, besides making bile for release in the duodenum. The venous network that absorbs n
Define the Interaction of Vitamin C with other Nutrients? Having gone through the discussion above it must be evident to you that nutrients are interdependent and are related t
Design a controlled experiment to test the hypothesis that acid precipitate inhibits the growth of elodea, a common freshwater plant.
Despite having a great biodiversity why is the Amazon Rainforest under risk of desertification? The natural soil of the Amazon Rainforest is not very fertile but it is enriched
define inflorescence and explain in detail about it''s types
What is Diatoms? Bacillariophyta are commonly known as diatoms because of their unique structure. These unicellular photosynthetic organisms are composed of two overlapping glas
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