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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Pure Culture Techniques We learnt the techniques involved in sub-culturing, i.e., the process involved in transfer of culture from one medium to another or transfer of
Proteins destined to be secreted from the eukaryotic cell are synthesized by ribosomes bound to the RER (rough endoplasmic reticulum). As the protein is synthesized it is transloca
Delimitation and Limitation: There may be many aspects of the problem that need to be explored, but it is difficult to cover all aspects in a single research study bec
Taxonomic Hierarchy The sheer complexity of organisms seems so bewildering that some people believe, life to be unfathomable by the rational mind. Biologists constantly chip aw
How did the experiments of Redi and Pasteur refute the hypothesis of spontaneous generation? To refute the impulsive generation hypothesis many experiments were performed. The
Define the term - lateralisation and localisation Discriminative validity studies including lateralisation and localisation achieved satisfactory results, but the localisation
Explain the Prevalence of iron deficiency anaemia? We can find out about the prevalence of anaemia if we know what percentage of population is suffering from anaemia. The WHO h
It is IgG mediated cytotoxic hypersensitivity. Typical manifestations contain erythroblastosis fetalis, hemolytic anemia, blood transfusion reactions etc.
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