Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What is physiologically corrected transposition of arteries, What is Physio...

What is Physiologically corrected transposition of arteries? Physiologically corrected Transposition of the great arteries (ZTGA): Atrio- ventricular connections are also inapp

Explain about round worms, Which one of the following statements about cert...

Which one of the following statements about certain given animals is correct? 1. Round worms (Aschelminthes) are pseudocoelomates 2. Molluscs are acoelomates 3. Insects ar

Explain phylum phytofagellata, Phylum Phytofagellata (Plant flagellates...

Phylum Phytofagellata (Plant flagellates) 1) At some stage of life cycle they have one or more flagella. The flagella are used for locomotion. 2) Photosynthetic protistan

How will this increase survival, Why would we evolve so that when we are st...

Why would we evolve so that when we are stressed our immune system is suppressed? How would this increase survival?

Hypocoagulation disorder and vitamin k deficiency, Which of the following p...

Which of the following patients likely faces the highest risk of an acquired hypocoagulation disorder and vitamin K deficiency? Answer A. A patient who is immunocompromised as a re

Infructescences- pseudofruits and parthenocarpic fruits, What are the infru...

What are the infructescences, pseudofruits and parthenocarpic fruits? The Infructescences are aggregated fruits formed from inflorescences, aggregated flowers. Grape clusters a

Eye testing and its significance, Eye complications are very common in pati...

Eye complications are very common in patients with diabetes. In fact, diabetes related eye diseases may not produce symptoms for a very long time and hence, it remains undetected t

Heat-shock response, Heat-shock Response When growing plantlets or tis...

Heat-shock Response When growing plantlets or tissues of plants are shifted to 42°C and above, the synthesis of normal proteins rapidly declines and instead a set of new prote

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd