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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Observation or Inference required for osazone test? 1. Crystals of different shapes will be seen. Glucose and fructose give needle shaped crystals and galactose gives f
Explain Food lipids It is either consumed in the form of "visible" fats, which have been separated from the original plant or animal sources, such as vegetable oil and butter,
How does a DNA vaccine prevent future disease? A DNA vaccine having DNA from a pathogen but cannot cause disease. When the vaccine is injected into a patient, the DNA directs t
Explain the Evolution of the Vascular Plant Body? Vascular Plant anatomy reflects adaptation to life on land. In an aquatic environment, the photosynthetic surfaces are support
In Reggie's case, he fractured the proximal end of his right femur, an integral component of his hip. Name the joint disorders that Reggie is more a risk of in (a) the short term a
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Effects of malnutrition The effects of malnutrition are not the same in adults and children. When the amount of food is not sufficient or when the nutrients are missing f
Q What are some examples of tissues and organs where mitosis is more frequent, less frequent or practically absent? Generally in vertebrates mitosis is more frequent in tissues
Issues Impacting Health and Development Poverty and malnutrition are the leading causes of high maternal and child mortality rates in developing countries. Together, they seri
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