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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Ulcerative enteritis (quail disease) Ulcerative enteritis, caused by Clostridium colinum, is found in chicken, quails, pheasants, turkeys and some other birds. Clostridial or
How does substrate concentration affect the initial rate of an enzyme- catalyzed reaction?
Define the Rehabilitation Phase of spinal trauma disease? Once the patient s condition stabilizes, individualized diets need to be recommended, based on the energy and nutrient
what are golgi apparatus
Life cycle of malarial parasite When the mosquito sucks the blood, gametocytes enter its digestive system. They migrate into the walls of the digestive system and undergo
plants
distinguishing charecters and classification of protozoa upto phyla
Q. Why are glucorticoids used in transplant patients? In the treatment and prevention of this common problem patients are given other immunosupressants or glucorticoids, Glucoc
How you can Repair Ventricular Aneurysm with a patch? Repair of Ventricular Aneurysm with a patch : Originally described by Cooley, as endoaneurysmorra
How many pints (473 ml) of Fat Tire amber ale can an 80 kg person drink in Arizona before reaching the blood alcohol limit? Fat Tire's ethanol concentration of 5.3% (v/v). Assume c
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