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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the gradient theoryof experimental embryology
1. A pure-breeding black, smooth guinea pig was mated to a pure-breeding white, rough female. They produced several litters, and eventually, five males and twenty females resulted
The allele that causes albinism (p) is recessive to the allele for normal pigmentation (P). A normal woman whose father is an albino marries an albino man whose parents are both no
Relate the oxide layer and biocompatibility of titanium. The compatibility of a metal with its host environment depends on its resistance to biodegradation and on the degree of
Types of Amoeboid Movements As the amoeba's cell body throws out one or a few pseudopodial lobes, a temporary rear end or uroid is pulled along. The central, more fluid protop
Q. Dietary management during atherosclerosis? Dietary management and the nutrient requirements during atherosclerosis remain the same as for the management of dyslipidemia. Hen
Seed gums Polysaccharides derived from plants and seeds have been in use for thousand of years. Seed gums are a group of polysaccharides with the following properties: S
Explain Dough Formation Gluten is the protein found in wheat. A unique property of gluten proteins of wheat grain endosperm (and to a lesser extent of rye and barley g
During final stage of cell division, mitotic apparatus disappears, chromosomes become attenuated, centrioles duplicate and split, nuclear membrane becomes reconstituted and nucleol
Q. What are target organs of the hormones? Target tissues, Target organs and target cells are those specific organs, cells and tissues upon which each hormone produces and acts
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