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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is Coevolution ? There is considerable evidence that supports an interesting theory that two individual species can affect each others evolution in reciprocal fashion. In
Limitations of Computed Tomograpy Scan This technology is costly Increased amount for radiation
How many cells are in the human body? According to "The Handy Science Answer Book" noted by the Science and Technology Department of the Carnegie Library of Pittsburgh (1994) th
parasitic adaptations
What is pcr?
Explain Membrane Filtration Technique? Another method used for determining the bacterial number is the filtration technique. The margain figure illustrates the membrane filter
What is Respiratory System explain their functionality ? The respiratory system is responsible for the transport of oxygen and carbon dioxide from outside the body to and from
Blunt Injury Closed penumothorax Tension pneumothorax Tracheobronchial injury Fracture ribs and flail chest Mediastinal injury
Define Integrity of cartilage Function of Manganese? Mn plays an important role in proteoglycan biosynthesis, which is essential for the integrity of cartilage. Bone defects ha
Explain the factor Chromosomal Inheritance? A huge step forward in our understanding of heredity came in 1902, when a biologist named Walter S. Sutton proposed that Mendel's "f
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