Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What is centrioles in cell structure, What is Centrioles in cell structure?...

What is Centrioles in cell structure? Most cells of higher organisms contain pairs of cylindrical bodies called centrioles, which help to organize microtubules in cell division

Cattle and buffalo diseases, C a ttle and buffalo diseases Rinderpes...

C a ttle and buffalo diseases Rinderpest: Rinderpest is the most destructive of the virus diseases of cloven-footed animals, viz. cattle, buffaloes, sheep, goats, pigs and

Dry stigma - category of stigma, Dry Stigma - Category of Stigma The C...

Dry Stigma - Category of Stigma The Cotton (Gossypium hirsutum) stigma is covered with long unicellular hairs. At the time of pollination, the stigmatic hairs show a distinct

How many carbon dioxide molecules are liberated, How many carbon dioxide mo...

How many carbon dioxide molecules are liberated after each cycle of the Krebs cycle? For a single glucose how many carbon dioxide molecules were already liberated by the aerobic re

Define dietary management of sepsis with or without mods, Define Dietary Ma...

Define Dietary Management of Sepsis with or without MODS? Before we begin with the dietary management of patients suffering from sepsis with or without MODS let us read a case

Role of nacl in metabolism, ROLE OF NaCl - It forms .9% of blood of ...

ROLE OF NaCl - It forms .9% of blood of mammal. It forms .7% of blood of frog. Bicarbonates of Na+ act as buffer for pH constancy. Involved in active transport of gluc

Illustrate sequential phases of the cell cycle, Q. How does the quantity of...

Q. How does the quantity of genetic material differ within the cell during the sequential phases of the cell cycle? The first period of the first phase interphase of the cell c

General guidelines of maintaining records of patients, General Guidelines ...

General Guidelines While working in the ward, you are always required to record. You should follow the general guidelines while recording which are as follows: All entri

What essential function do they serve, Why do membranes figure so prominent...

Why do membranes figure so prominently in eukaryotic cells? What essential function do they serve?

Environmental accounting, Valuation of biological resources includes the pr...

Valuation of biological resources includes the process of deriving a monetary value to the things that are not sold in the market. Examples are the value given to fuelwood gathere

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd