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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Describe the Complete transposition of great arteries ? Lethal, relatively frequent malformation. Without treatment, it results in 30 per cent mortality within the first week o
African horse sickness African horse sickness (AHS) is a highly fatal insect born viral disease of equidae caused by an Orbivirus (family Reoviridae) and characterized by sever
Describe how many bones are there in human foot The human foot is a great mechanical marvel. It has 26 bones, 29 joints, and 42, various ligaments, a sensitive and protective s
Who created the two-part naming system used in biology? The scientific naming system that is used worldwide today was primary devised by Swedish naturalist Carl Linnaeus in 173
Q. What is Constipation? Constipation is irregular, infrequent or difficult passage of faeces. It is the most common physiological disorder of the alimentary tract. It is chara
Abdomen check the contour of the abdomen; normally it should be soft, symmetrical, slightly round and moves in synchronously with chest in premature neonate abdomen w
Determine Minerals Requirements at high altitude? Increased urinary excretion of Nat and K + on exposure to hypoxia is reported while some workers have found only increase in
Describe about Congenital Cardiac Conditions Encountered in Adults ? The entire population of adults with CHD is constituted by patients whose cardiac malformations have a natu
This assignment is a 1500 word 'clinical update' journal article. The format for this article is similar to that of the 'ANF clinical update' series published in the Australian Nur
Define about the Photometry - colorimetry? Photometry is the measurement of the luminous intensity light or the amount of luminous light falling on a surface from such a source
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