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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Classification of plants and animals
Q. Active Listening - counselling skills used in diabetes? Every day we listen to many things, may be not with any intention or paying attention to the words, speech etc. For i
How does the odor of flower petals help pollination? The purpose of the perfume is to attract a pollinator - insect, bat, bird or whatever. The reward for the pollinator is a m
Pre-Diabetes is a condition where blood sugar level is above normal but below the value to be diagnosed as diabetes mellitus. It has been found that those people with blood sugar v
Oxygenator : They serve the function of lungs during extra corporeal circulation (ECC) - Oxygenation, removal of carbon dioxide and transport of gaseous anaesthetic agents:*The o
HORMONE S OF PANCREAS AND THEIR ROLE - (i) Glucagon (Secreted by a-cells) It stimulates the liver to convert stored glycogen into glucose. Glucagon is controlled b
Ecology and Ecosystem The concepts of environment, ecology, ecosystem, energy flow and nutrient cycling in the foundation course on Science and Technology. As you are aware ec
Define the Use of Computers in Numerical Taxonomy The use of computers in numerical taxonomy has made it possible to compare a large number of characters from many organisms wi
Will there be an opportunity to comment? In a supplemental notice accompanying the last rule package, EPA is soliciting additional information and public comment on issues fro
#question.endocrine organ in crustaceans
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