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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Elephant Trunk Technique in Aortic Aneurysm ? Elephant Trunk Technique : When aneurysm involves arch of the aorta and large portion of descending thor
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Q. What are the homologous chromosomes? Which are the human cells that do not have homologous chromosomes? Chromosomes contain genes genetic information in the form of nucleoti
How we explain intenal organs when we talk about symmetry??Is there any animal that is completely symmetric even insid the body?
Q. Function of Dopamine in consciousness? An increase in dopamine activity produces an increase in wakefulness. Dopaminergic neurons in the ventral tegmental areas are constant
Q. What are the major human diseases caused by fungi? The main human diseases caused by fungi are paracoccidioidomycosis, histoplasmosis, blastomycosis, coccidioidomycosis, or
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What are some industrial processes that use bacteria? Bacteria are used by industry in many ways. There are vaccines made of attenuated pathogenic bacteria or of antigens prese
Define absorption, transport and excretion of magnesium? Magnesium absorption to some extent is similar to that of Ca. Absorption of Mg occurs throughout the small intestine, a
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