Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Define main gastric digestive enzyme, Q. Besides being fundamental for the ...

Q. Besides being fundamental for the activation of the main gastric digestive enzyme how does HCl also directly participate in digestion? With its corrosive effect HCl also hel

Zoonotic diseases-influenza, Influenza Influenza is an acute infectious di...

Influenza Influenza is an acute infectious disease caused by influenza viruses of genus Orthomyxovirus in family Orthomyxoviridae. The name Influenza is derived from an Italian ph

#biology tutor title.., When nd how i can srt my online work in this site q...

When nd how i can srt my online work in this site question #Minimum 100 words accepted#

Tracheae - respiratory organs, Tracheae - Respiratory Organs Tracheal ...

Tracheae - Respiratory Organs Tracheal respiration is characteristic of insects. Onychophorans, diplopods, arachnids, and chilopods as well use tracheal system for respiration

Explain main functions of organic molecules for living being, What are the ...

What are the major functions of the organic molecules for living beings? Organic molecules, such as proteins, lipids and carbohydrates, perform numerous functions for living or

Explain Objectives of root-end resection apicoectomy, Explain Objectives of...

Explain Objectives of Root-End Resection Apicoectomy a) Removal of untreated apical portion of the root, b) Enable the obturator to determine the cause of failure,  c) To

Explain about gastro oesophageal reflux disease, Q. Explain about Gastro Oe...

Q. Explain about Gastro Oesophageal Reflux Disease? GERD refers to the regurgitation of acidic stomach contents into the oesophagus. It results in a spectrum of clinical manife

Enzyme activity, Enzyme Activity Enzymes are biomolecules which catalyz...

Enzyme Activity Enzymes are biomolecules which catalyze (i.e. increase the rates of) chemical reactions. Almost all enzymes are proteins. In enzymatic reactions, the molecules

Define methods of food processing, Define Methods of Food Processing? F...

Define Methods of Food Processing? Food is undeniably most vital to the survival of human beings. Hence, it must be processed using various scientific techniques. This is done

What proportion of children with down syndrome found, What proportion of ch...

What proportion of children with Down syndrome do you expect when women with down syndrome have children with men who have 46 chromosomes? Justify answer

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd