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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What are the cells produced in the first stage of the embryonic development called? The cells that result from the cleavage (the first stage of the embryonic development) ar
Pregnancy toxaemia in sheep (twin lamb disease, sleeping ewes disease, pregnancy ketosis). Pregnancy toxaemia is a multifactor disorder of energy metabolism affecting sheep du
Define Obesity - Excessive Fat Intake? In obesity, cutting down total energy intake or increasing output to ensure energy balance is the basic principle of prevention. Fat bein
GER M LAYER THEORY - Proposed by Pander, studied chick embryo development. Coined the term ectoderm, mesoderm & endoderm.
Define the Food and Nutrition Security? In the previous section, we learnt about the trends in production of food grains in the country and their relationship to population gro
A 37-year old woman is admitted to the hospital after complaining of chest pains. She admits to having had severe headaches for several days prior to seeking medical help. She is a
Define Mutual supplementation of Protein with legume and milk? Improvement of cereal diets with legume and milk proteins: The proteins of poor diets based on different cereals
Hemiplegic migraine and ophthalmologic migraine These are relatively rare and include loss of movement of the limbs and eyes, respectively.
Select the processes assist in the maintenance of high levels of dissolved solutes in the interstitial spaces of the kidney medulla? A. Net flux of sodium ions from intracellul
E s o pha g e a l obstruction It is also known as choke. It may be acute or chronic and is characterized by inability to swallow, regurgitation and bloat. E t
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