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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
what are alleles
In vitro fertilisation is a technique that involves transfer of which one of the following into the fallopian tube? 1. Embryo only, upto 8 cell stage 2. Either zygote or ear
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Q Why do protease-supplying cells of the stomach and of the pancreas make only precursors of the active proteolytic enzymes? The stomach and the pancreas make zymogens of the p
#qprokroyotic flagella structure and protocoluestion..
Partial Pericardiectomy : The approach can be either by a left anterolateral thoracotomy or median sternotomy. For pyogenic pericarditis with adhesions and loculations a par
What is the location of the salivary glands in humans? There are 6 major salivary glands and they are located one in every parotid gland, two beneath the mandibles (submandibul
Q. Why iodine is vital for human beings? Iodine is a essential chemical element for the proper functioning of the thyroid since it is part of the hormones produced by this glan
What are seed gums? What are its characteristic properties? Seed gums are a group of polysaccharides with the following properties: light to dark cream colored amorphou
Fatty acids are synthesized in the cytosol, but acetyl CoA is produce from pyruvate in the mitochondria. Therefore the acetyl CoA must be transferred from the mitocho
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