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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is inharmonious ecological interaction? The Inharmonious or negative ecological interaction is that in which at least one of the participating beings is harmed.
You measure the stability of your favourite protein kinase and find that half of the protein is degraded every 10 minutes. How might you test whether the protein is degraded throug
Renal Glycosuria Renal glycosuria is a condition in which the glucose is excreted in the urine in spite of normal blood sugar levels. Self Monitoring of Blood Glucose (SMBG
C dna is made from a mature m rna of eukaryotic cell with the utilization of enzyme called as reverse transcriptase. Steps in c dna creation 1. m rna is made and purified f
Parturient paresis (milk fever, hypocalcaemia) Parturient paresis is an acute to peracute non-febrile disease, which occurs in diary cows and buffaloes usually around the t
Q. Prevention strategy for diverticular disease? The prevention strategy for the disease involves the following: • Eat a high-fiber diet (more than 15 g/day of crude fiber)
Photoreceptors Photoreceptors are concerned in absorption of light by photosensitive pigments. The chemical change involved produces the impulse concerned in the nerve cells.
What is Resection and Primary End to End Anastomosis ? For neonates and infants the best operation is resection of coarctation and primary end-to-end anastomosis. With the baby
Why Pulses are important for human - nutritional factor? Pulses are rich sources of proteins (20-25 g/100 g), the limiting amino acid being methioniize. However, protein quality
characteristics of class mamalias
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