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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Nicotinamide nucleotides These coenzymes are derived from the vitamin, niacin. They are of two types, nicotinamide adenine dinucleotide (NAD') and nicotinamide adenine dinu
If carbon 1 is the carbonyl group of a 6-carbon aldose (aldohexose), which carbon determines if the sugar is a D- or L- stereoisomer? Select one: a. 1 b. 2 c. 3 d. 4
Isonzerization of glucose-6-phosphate Isonzerization of glucose-6-phosphate : This step is catalyzed by phosphoglucoisomerase to form fructose-6-phosphate.
Hi, I have this presentation about the kingdom of fungi, and no website could clearly explain the two processes of fragmentation and sporulation. I mean what are their steps? And w
Stem cell research has been promoted as a future medical cure-all for a host of diseases, including Alzheimer's and diabetes, as well as a potential breakthrough for organ replacem
What is the phenomenon of the apical dominance in plants? How it can be artificially eliminated? The Apical dominance is the phenomenon by which high (over the positive range l
Genital warts and human papillomavirus (hpv) infection External genital warts are caused by human papillo- mavirus, usually type 6 or 11; other types (16, 18 and others) cause
Some hyperthermophilic organisms that grow in highly acidic (pH2) habitats belong to the two groups: 1. Eubacteria and archaea 2. Cyanobacteria and diatoms 3. Protists and
Wheel organ of amphioxus.
Define Prevalence of Weight Management? WHO (1998) estimates that in developing countries about 245 million adults are moderately underweight and 93 million severely underweigh
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