Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Cleavage, what are the chemical changes takes place during cleavage

what are the chemical changes takes place during cleavage

using a two-sample t-test , After stinging its victim, the honeybee leaves...

After stinging its victim, the honeybee leaves behind the barbed stinger, poison sac, and muscles that continue to pump venom into the wound. A study compared two methods of removi

Leishmanias – flagellates, Leishmanias – Flagellates Species of the ge...

Leishmanias – Flagellates Species of the genus Leishmania are parasites of mammals including man. Leishmanias infections occur over wide regions of the world from Asia, the ne

Explain the barrier function in the cornea, Explain the barrier function in...

Explain the barrier function in the cornea. Barrier Function: It is the first line of barrier between the external environment and the corneal stroma. The superficial epi

How many hours will the bag last, Mr. Jones I.V. was hung at noon. It is a ...

Mr. Jones I.V. was hung at noon. It is a liter bag and is infusing at 65 ml/hr. How many hours will this bag last?

Gametogenesis - human development, Gametogenesis - Human Development G...

Gametogenesis - Human Development Gametogenesis as you are responsive is the process of formation and development of specialized reproductive cells, ova in females and sperms

Define effect of vitamins (a, Define the effect of Vitamins (A, D, K and B-...

Define the effect of Vitamins (A, D, K and B-Complex) on athletes? Vitamins A, D and K have been found to have no ergogenic effects. Ingesting large doses of these vitamins hav

Fertilisation, Fertilisation: Fertilisation is the process of fusion...

Fertilisation: Fertilisation is the process of fusion of male and female gametes. For fusion of male and female gametes, pollen grains have to reach the surface of the stigm

Reaction - processes in succession, Reaction - Processes in Succession ...

Reaction - Processes in Succession This is the most important stage in succession. The mechanism of modification of environment, through the influence of living organisms on i

Porifera - regeneration in invertebrates, Porifera - Regeneration in Invert...

Porifera - Regeneration in Invertebrates Sponges have immense regenerating ability and show two ways of doing so as displayed below. : (a) Regeneration from small segments-

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd