Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Where in the body is the thyroid gland located, Where in the body is the th...

Where in the body is the thyroid gland located? The thyroid is located in the anterior cervical region (frontal neck), in front of the trachea and just below the larynx. It is

How tyndallisation used for sterilization, Q. How Tyndallisation used for s...

Q. How Tyndallisation used for sterilization? John Tyndall devised a process of sterilisation by steaming for a few minutes at 100 o C on 3-4 successive operations separated by

Types of leukemia, Types of Leukemia Leukaemia may be chronic  or acut...

Types of Leukemia Leukaemia may be chronic  or acute and is classified  into  the  following  categories:  i)  Acute Lymphocytic Leukaemia (ALL) This is  the most comm

Respiration, what is known as respiration in animals?

what is known as respiration in animals?

Explain bone healing process in nutritional care, Explain Bone Healing Proc...

Explain Bone Healing Process in Nutritional Care? Protein is essential for callus formation, calcification and bone healing especially in cases of orthopaedic surgery. The prot

Which part of the teeth is it important to remove plaque, From which part o...

From which part of the teeth is it particularly important to remove plaque. It is most significant to remove plaque from among the teeth and from the region where the gum cover

Urology., what is the effect of KClO4 on the kidneys in the nephrotic syste...

what is the effect of KClO4 on the kidneys in the nephrotic system?

Infection - complications of cardiac surgery, Infection Due to the m...

Infection Due to the many invasive monitoring techniques, ET tube, urinary catheter, surgical interventions. Cultures of blood or sputum/urine/swab form the wound is don

Explain basal metabolism rate (bmr) - ageing, Explain Basal Metabolism Rate...

Explain Basal Metabolism Rate (BMR) - Ageing? From age 25 years, the basal metabolism decreases by about 2 percent for each decade due to the increasing proportion of body fat

Why is photosynthesis referred to as a biochemical pathway, a) Why is it si...

a) Why is it significant that ions being transported across a cell membrane be shielded from the interior of the lipid bilayer? b) Why is photosynthesis referred to as

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd