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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is coronary bypass graft? The Coronary artery bypass graft is a kind of surgical myocardial revascularization that is a way to provide blood to a myocardium whose blood
(a)State the law of independent assortment. (b )Using punnett square demonstrate the law of independent assortment in a dihybrid cross including two heterozygous parents.
different types of respiration
Which of the below refers to science concerned with the application of genetic principles to improvement of the human species? a) Eugenics b) Assortive mating c) Holandri
what is the classification of protozoa
What is a cytoskeleton? What are its main constituents in animal cells? Cytoskeleton is the cytoplasmic structure that handles the cell, keeps its shape and fixates and moves t
project based learning
Define Micronutrients Requirements for Anorexia Nervosa? It is advisable to include one 100% RDA multivitamin tablet with minerals. The use of prophylactic thiamine supplements
Define the Criteria for an Indicator Organism? Following criteria are suggested for an indicator organism: i. It should be suitable for the analysis of all types of water, i
Horse-pox Horse pox is a benign disease characterized by the typical pox lesions on the lower limbs or around the muzzle and buccal mucosa. This is a rare disease, caused by a
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