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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Food Applications of alginate One of the most unusual properties of the alginates has been the ability of soluble alginate salts to produce attractive, edible gels or jellies.
Loading and Unloading of Sieve Tubes In order to understand the loading of food from manufacturing leaf cells to sieve tubes we must examine the anatomy of a minor vein shown
THEORIES OF AGEING - 1 . Compromise theory. 2 . Wear & tear theory. 3 . Somatic mutation theory. 4 . Immunity theory - Thymus becom
D i s e a s e s of Cardiovascular and Haemopoietic System Cardiovascular system maintains circulation of blood for normal exchange of fluid, electrolytes, oxygen and nut
WHAT is zoonosis?
What is scientific investigation?
Which of the following statements concerning enzymes is false? a. They function as catalysts b. The active site of an enzyme has a shape that is specific for its c. Many e
What is the significance of proteins for living beings? Proteins play a fundamental role in nearly all biological processes. Because of their diversity they can assume many dif
F A TT Y ACIDS They are monocarboxylic organic acids (R.COOH) having a hydrocarbon chain of 4 - 30 carbon atoms. The polar carboxylic group is hydrophilic (Gk. hydro
ECG: Myocardial ischemia is indicated by T-wave inversions. Left ventricular hypertrophy (LVH) criteria: R in aVL >13 mm R voltage in L1+ S III >25 mm S v1 + R v5 or v6
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