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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain about the water-soluble vitamin - Riboflavin? Riboflavin (vitamin B 2 ), a water-soluble vitamin, was discovered in milk as a pigment possessing a yellow green fluore
Pre-procedural mouth rinse Patients' use of a an anti-microbial mouthwash of 0.12% chlorhexidine gluconate solution for 30 seconds prior to intra-oral procedures will reduce th
What happens to transcription at the lac operon when lactose is absent and why? What happens to transcription at the lac operon when lactose is present and why? What happens
What is a trace element and how are they important in organisms, give an example.
.respiration in animals
Foot-and-mouth disease Foot-and-mouth disease (FMD) is an extremely contagious viral disease of cloven footed animals most notably cattle, pig and sheep. It is well known for
Define Agar Syringe/Agar Sausage Method? Agar syringe method involves 100 ml syringe, which is filled with agar. A layer of agar is pushed beyond the end of the barrel by means
Three point charges are located at the corners of an equilateral triangle as in the figure below. Find the magnitude and direction of the net electric force on the 1.40 µC charge.
Some activities that make use of biological resources, without involving their extraction or destruction, denote non- extractive use of biological resources. This includes ecotouri
High Energy Compounds Primary need of the animal body from the nutrients is energy. Free energy is the term used to denote the energy available to do useful work. Anabolic or synth
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