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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Is there anything I can do to keep my brain healthy in older age? It is increasingly clear that how we live our lives on a daily basis strongly influences how well our brain ag
Define need of vitamin E and K during pregnancy period? Vitamin E needs are believed to increase during pregnancy but deficiency in humans rarely occurs. Vitamin E in the infan
advantages of cuticle of anthropodes
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1. In which layer of skin are follicles usually found? 2. How are sebaceous glands associated with hair follicles? 3. In which layer of skin are sweat glands usually located?
Will there be an opportunity to comment? In a supplemental notice accompanying the last rule package, EPA is soliciting additional information and public comment on issues fro
The various organs involved in the digestion process are: Alimentary tract which includes mouth, pharynx, esophagus stomach, small intestine, large intestine, appendix, rec
Q. What are the major divisions and representing species of the gymnosperms? This group of plants can be separated into conifers (pine, sequoia, cypress), that have flowers cal
Problem: Evaluation of disabilities due to occupational diseases (a) Describe the following terms: "impairment", "disability "and "handicap" (b) Show how the evaluation o
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