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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
BASICS OF ENVIRONMENT We live in two world one is natural world of animals, plants, air, water and soil that was present since the evolution of earth. The other world is of soc
Describe Valvar Aortic Stenosis Murmurs ? Characteristic: Harsh, rasping crescendo-decrescendo murmur best heard anywhere in a straight line from right 2nd ICS to apex ("sash"
What is the Definition of Polyphenols? Polyphenols are the compounds that contain an-ON group attached to the benzene ring. The main classes are: flavonoids, phenolic acids and
1. Briefly explain the dynamics in enzyme reaction mechanism with potential energy surface diagram. 2. How to find the rate constant of any enzymetic reaction? 3. Derivation
MOD E OF HORMONE ACTION THROUGH EXTRACELLULAR RECEPTORS - The molecules of amino acid derivatives, peptides or polypeptide (protein) hormones bind to specific receptor molecul
Vitamin E (DL-α-Tocopherol) DL-α-Tocopherol is a yellow to red-brown, clear, viscous oil almost without odour which decolourise when exposed to light and air. DL-α-Tocopherol
Q. What are the major events of the final mitotic period? The final mitotic phase is the telophase. In the telophase the following events occur decondensation of chromosomes ea
What is meant by substrates of enzymatic reactions? Substrates are reagent molecules upon which enzymes act. The enzyme has spatial binding sites for the attachment of its s
P o x diseases Small-pox in human beings and pox in a few animal species are closely related to each other. It is shown by the fact that the vaccine for the prevention of sma
NE R VE IMPULSE (IT'S GENERATION) - It is an electro chemical phenomenon manifested through ionic movement across cell membrane. It was discovered by A. L. Hodykin & A
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