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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Pregnancy - Fungal Infections Amphotericin B is the preferred treatment for deep fungal infections during pregnancy. Fluconazole is teratogenic in animals and a same pattern of
Disease Y Study: Let us consider the Disease Y study, a brief description of the study is as follows. A case-control study was conducted of 75 cases with a rare genetic disorder "
Hydralazine is a potent arteriolar dilator and markedly increases cardiac output in patients with congestive heart failure. However, as a single agent, it has not been shown to imp
In a numeric pyramid to which trophic level does the base always refer? What about the top level? In a numeric pyramid the base corresponds to the first trophic level, i.e., to
Define Food Science as a Discipline and Modern Developments? As an undergraduate student you may have taken a course in food science and technology. Do you recall, what did the
What is the most abundant form under which nitrogen is found in nature? The most abundant nitrogen-containing molecule found in nature is molecular nitrogen (N2). The air is 80
Where in the leaves is photosynthetic tissue often located? The major photosynthetic tissue is the photosynthetic parenchyma (also known as chlorenchyma, do not confuse with co
Which level of a gene, such as the ADH gene and its encoded product should have the highest homology among different species? Explain your answer. A. The genomic DNA sequences o
what are the two reactants and the two products of a dehydration reaction/ What are the two reactants and products of a hydrolysis reaction
whats ur clear definition of virus
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