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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Specify the term in detail - Swim bladder. Found in bony fish, this gas-filled chamber is used to main neutral buoyancy. Oxygen in blood is added or removed as required. Swim b
Tapetum - Anther Wall Layers Tapetum is the innermost and most important layer of the anther wall. It is generally composed of a single layer of cells. These cells completely
Define Some Food Related Problems in parkinson's disease? As the disease progresses some food related difficulties appear. These are: 1. Difficulty in food preparation
Why is it more probable that the photosynthetic prokaryotes appeared before the aerobic eukaryotes? It is more feasible that photosynthetic prokaryotes appeared before the aero
At the present level of the biotechnology what are the main techniques of genetic engineering? The major techniques of genetic engineering today are: the recombinant DNA techno
Enzyme glutamate oxaloacetate transaminase catalyzes the reaction between glutamate and oxaloacetate with the formation of a-ketoglutarate and aspartate due to transfer of one
List and briefly describe the mechanisms that prevent acid-base imbalances from appearing in the body. In addition, indicate where each mechanism functions most significantly.
Define isolated soybean proteins in Seafood products? The most important application in this category is the use of ISP in fish sausage and surimi based restructured fish produ
how many chromosomes in human
Describe Metabolic Syndrome ? As discussed, the risk factors like obesity, high blood pressure, high blood glucose or impaired glucose tolerance, and dyslipidaeinia are very im
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