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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
in human and mammals how many cells result from oogenesis
What is vascular cambium? What are their functions? The Vascular cambium is the secondary meristematic tissue that in roots and in the stem forms the vascular tissues phloem an
Explain the Turbidity Measurement? Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estim
how do honey bees breathe
Nature: Matter and Energy Nature is not just our immediate surrounding or environment, but is the whole Universe of Cosmos. Study of the origin and topography of cosmos is ca
Q. What are the typical components of a closed circulatory system? The typical components of the closed circulatory system are the blood vessels within which blood circulates c
What is drugs? Intravenous access must be established. Although administration of drugs through a central vein is ideal in a low cardiac output situation, it is rarely possible
PRINCIPLES OF DARWINISM - 1 . OVERPRODUCTION (PRODIGALITY) - All organisms have enormous fertility and they multiply in geometric ratio. Examples of over product
Soil pH and Nutrient Availability Soil pH is the most important factor which governs the availability of nutrients in soil. All the nutrients are absorbed by plants in their io
Kidney - 2 in number. Dark red. Slightly flattened. Bean shaped. 10 cm. long, 5 cm wide and 4 cm thick (In rabbit 20-25 mm). Weighing 150 gm in male 135 gm in fema
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