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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Pericardiocentesis It is removal of fluid form the pericardial sac. It is a specialized procedure done n ICU or cardiac cath lab or OT. A 16 or 18 gauge needle is inserted
a) Explain what is meant by ovulation. b) How often does it happen in humans? (a) Ovulation is the release of an ovum from a mature follicle in the ovary.
For many years, students who have occupied my Molecular Biology course have now applied for lab tech jobs exact after graduation. But I have NEVER had a student fail to obtain a jo
sanguivarous mode of feeding in annelida
What are the processes of the photochemical stage of the photosynthesis process? Photolysis of water, with liberation of molecular oxygen, and photophosphorylation of ADP, with
How much ampicillin (sodium sal, mw=371.40) would you dissolve in 400 mL of water to make 80 mg/ml solution of ampicillin?
What are the typical vegetation and the typical fauna of the tundra? Tundra has vegetation formed mainly by mosses and lichens. In the fauna the densely furred animals, like ca
How are the main classes of arthropods classified according to the presence of larval stage in their embryonic development? In crustaceans there are species with direct and oth
For the biological diversity is mitosis or meiosis the more important process? Meiosis is the cell division process that permits the formation of gametes to sexual reproduction
Define the Insulin resistance - Obesity? Insulin resistance is a condition in which your body cells cannot utilize insulin efficiently although sufficient amounts are secreted
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