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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Why can the amphibian circulation be classified as incomplete and double? The amphibian circulation is double because it is composed of pulmonary and systemic circulations r
Explain about the Adolescent Pregnancy? The pregnant adolescent is viewed as a high-risk patient, highly susceptible to suboptimal pregnancy outcome. Even when not pregnant, a
Plants - Slow Moving Waters Plant life is abundant in this habitat and includes rooted vascular plants such as pond weeds and grasses, firmly attached aquatic mosses and multi
Define the Diet intervention for lactose intolerance? Lactose is present in dairy products such as milk, cheese, yoghurt, ice cream etc. Hidden sources of lactose may include
what are floating plants? detailed information
What is the function of the skin in humans? The skin is the external covering of the body. In humans its major functions are protection, perception of information from the envi
Q. Definition of Osseointegration in microscopic biophysical? Osseointegration implies that at light microscopic and electron microscopic levels, the identifiable components of
What is pollen? Pollen grains are the male gametophytes of the phanerogamic (flowering) plants. Thus within the pollen grains the male gametes of these plants are produced by m
biting mechanism in snakes
The acidity and alkalinity (pH) of an environment has a strong influence on the activity and stability of macromolecules such as enzymes. These enzymes play an important role durin
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