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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Consistently overwatering a potted tomato plant will eventually kill it. Using the map, suggest why waterlogged soil results in plant death. O2 cannot reach respiring root cells. T
Alfred Sturtevant and Seymour Benzer's studies of recombination in both Drosophila and bacteriophage demonstrated which of the following? A. The gene is an indivisible unit and
Explain the Objectives of Congenital Heart Disease ? After reading this unit, you should be able to: 1 Comprehenced the epidemiologic significance of congenital heart disease (
Structure of human spermatozoon: Nature: Human sperms are minute, microscopic and motile. Structure: Each sperm have an oval shaped head piece, a neck, a middle piece and
An ecosystem includes
Q. What is meant by unsaturation or saturation of oils and fats? When it is said that a triglyceride is saturated it means that in its molecule the carbon chain is bound in its
What are the alleles of a gene? The Diploid individuals have paired chromosomes. For instance in humans there are 23 pairs of chromosomes totaling 46 chromosomes. Every pair co
Body Fluids of Marine Teleost The body fluids of marine teleost are hypotonic to seawater, so these fishes lose water to the environment especially across the gill epithelium.
Biota of Pelagic Zone Pelagic region constitutes 90 per cent of the total ocean surface and is less rich in species and numbers of organisms than the two regions discussed bef
Q. Rules of Nomenclature classification? The Rules of Nomenclature prescribed the categories into which plants should be classified. These categories constitute the units of cl
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