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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Can you explain Ventricular Septal Defect? VSD can be found in various part of ventricular septum hence all possible views except suprasternal views are used to detect an
Asymptomatic patient: The current opinion is that in asymptomatic patients with left ventricular dysfunction and severe MR surgery should not be delayed. The echo cardiographic a
functions of iron in the body: Iron is the major component in haemoglobin. It helps to carryout oxygen from lungs to the tissues. About 60 to 70% of Iron in the body i
Physiological Changes - Consequences of Aging Various physiological regulatory mechanisms show decreased efficiency due to aging. For instance, normally the glucose level in t
Q. What is the function of the feet in molluscs? How is the mollusc foot related to the name given to the classes of the phylum? The mollusc foot has the function of support, l
Cell - Cell is the structural & functional unit of life. Study of the structure of the cell is called cytology. Study of structure and functions of the cell is called
Q. What are anticoagulants? What are the practical applications of anticoagulants, like heparin, in Medicine? Ordinarily there are anticoagulants circulating in the plasma sinc
What is the cost-benefit relationship regarding sewage treatment as a method to fight water pollution? To treat sewage is much cheaper for society. The non treated sewage pollu
when the father and the mother of a newborn baby possesses blood group ''O'' +ve,is it possible that the baby can have a blood group ''A'' +ve or ''B''+ve ? Conventionally it shoul
I want to do assignment on kerb cycle with in text citation
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