Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Explain the shear or moment diagrams, Explain the Shear or Moment Diagrams ...

Explain the Shear or Moment Diagrams Shear diagram changes by magnitude of the load. Shear is constant along unloaded portions of a beam For a point load, the she

Is vacuoles easily found in fresh or in salt water, Are protozoans presenti...

Are protozoans presenting contractile, or pulsatile, vacuoles easily found in fresh or in salt water? Fresh water is the less concentrated of solutes than sea water and it (fre

Define reflection - endodontic surgery, Define Reflection - Endodontic Surg...

Define Reflection - Endodontic Surgery -  Marginal gingival is very delicate and easily injured, therefore you should begin reflection of the vertical incision in the attached

What are seed gums, What are seed gums? What are its characteristic propert...

What are seed gums? What are its characteristic properties? Seed gums are a group of polysaccharides with the following properties: light to dark cream colored amorphou

Bacteria, Bacteria The study of bacteria is called bacteriology. Bacte...

Bacteria The study of bacteria is called bacteriology. Bacteria are unicellular organisms and possess a distinct cell wall. They are included in kingdom Prokaryote. The bacter

Describe the structure of lipoprotein, There are several different lipoprot...

There are several different lipoprotein species found in plasma but their basic structures are similar. The insoluble lipid (cholesterol ester and triglyceride) forms a central cor

What is the meaning of chelicifore, What is the meaning of Chelicifore? ...

What is the meaning of Chelicifore? These feeding mouthparts are found on most, pycnogonads. Though the proboscis is the main feeding structure in these animals when chelicifor

Define meitrac system instrument removal system, Define Meitrac System Inst...

Define Meitrac System Instrument Removal System a) Trephining bur to remove dentine and uncover the coronal end of the file b) Extractor of appropriate size is placed over t

Fungi, Candida albicans, and Aspergillus species are the most common of the...

Candida albicans, and Aspergillus species are the most common of the many fungal organisms identified as causing IE. Fungal endocarditis arises in specific settings. Valve replace

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd