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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Can you explain about Cardiomyopathies? Cardiomyopathy is a primary disorder of heart muscle that may cause cardiac dysfunction and is not related to any obvious disease pr
Explain the Procedure for Testing Quality of Water Using Presumptive Test? Now carry out the exercise following the steps indicated herewith. 1. Make 3 series of lactose bro
.respiration in animals
Planning of Nursing Care Prevent and control bleeding. Administer replacement therapy of factor VIII and IX as advised. Educate and support the patient and family.
What do you understand by Compound eye? The characteristic eye of the Arthropoda. It comprise many ommatidia, the basic optical unit of compund eye, grouped together to form th
Which of the following is true for the ATP-sensitive potassium channel? A. The channel is a spanning protein with a receptor site for ATP located on an extracellular region of
Define Obligatory and Facultative water excretion? i) Obligatory water excretion: The kidney is 'obligated' to excrete some water to rid the body of its daily load of urinary s
Q. What are the major features of fishes associated to the habitat where they live? Fishes are all aquatic animals and thus they have a hydrodynamic elongated body appropriate
When a piece of thread is tied tightly around an animal pancreatic duct the animal experiences difficult in digestion but does not suffer from diabetes mellitus.Explain why?
What is Class Reptilia ? Class Reptilia include the vertebrates that have successfully transitioned to a terrestrial lifestyle by evolving protective scales and a "land egg."
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