Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Procedure for use microbiological laboratory equipment, Define Procedure fo...

Define Procedure for use Microbiological Laboratory Equipments? Now carry out this activity following the steps given herewith: 1. Observe each instrument (as mentioned abov

Explain holding method of pasteurization, Explain holding method of pasteur...

Explain holding method of pasteurization In the holding method of pasteurization (62 o C for 30 minutes) or the high-temperature short-time (HTST), 71 o C for 15 minutes method

Which are the brain regions associated with memory, Which are the brain reg...

Which are the brain regions associated with memory? According to researchers some of the main regions of the nervous system associated with the memory phenomenon are the hippoc

Devonian, Devonian  is the period of geologic time from 410 - 360 million y...

Devonian  is the period of geologic time from 410 - 360 million years before the present. Life on land diversified, with amphibians appearing late in this period of time. Plants we

Define the post-herbal period, Define the Post-Herbal Period? It is di...

Define the Post-Herbal Period? It is difficult to draw a sharp line of demarcation between the transition period, marked by various attempts of classification, all of which we

#sponges, expanciate the process of oxygen and carbon dioxide exchange in s...

expanciate the process of oxygen and carbon dioxide exchange in sponges

What is pulmonary venous congestion ?, Q. What is Pulmonary Venous Congesti...

Q. What is Pulmonary Venous Congestion ? In pulmonary venous hypertension, the earliest change is an increase in calibre of the upper lobe vessels. If the upper lobe veins meas

Compare between isp and soy flour, Compare between ISP and soy flour Th...

Compare between ISP and soy flour The cost of isolated soybean proteins is five to seven times higher than that of defatted soy flour. On an equivalent protein weight basis, th

#title.zoology., #question.why obelia is considered of special interest in ...

#question.why obelia is considered of special interest in zoology.

Reptiles, biting mechanism in snakes

biting mechanism in snakes

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd