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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Say about thikka desease of ground nut
What are mycorrhizas? How does each participant benefit in this ecological interaction? Mycorrhizas are mutualist ecological interactions among fungi and some plants roots. Fun
ECONOMI C IMPORTANCE OF WILD LIFE - (i) Plants - Produce food. Provide Timber, paper, gums, drugs, pigments, fibres, rubber, oils, condiments & fuel. Release O
A foreign DNA has to target a precise chromosomal site and integrate with the host DNA. Are the methods of gene therapy strong enough to produce desired expression without mistakes
Your breakfast consists of a cup of black coffee with sugar as well as a plain bagel covered with cream cheese. Describe the digestion of this breakfast as it passes through each m
Define about the Physical Fitness? Physical fitness is a general term we come across quite often and use it to describe an individual if he or she is able to carry out a variet
Neurons - Nervous System Although neurons occur in a great variety of sizes and morphological forms, they have a basic plan of structure representing certain common characteri
Q. Indications for Surgery of mitral regurgitation? Surgery is indicated in all symptomatic patients (class II and above) with severe mitral regurgitation and normal or decreas
what is the largest nerve centre
middle man of the body
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