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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Name two possible why the number of live bacteria cell have reached the stationary growth by 60hrs and start to die off after 12hrs?
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Endodontics principles and practice According to American association of endodontic, retreatment defined as:A procedure to remove root canal filling materials from the tooth vi
Q What are the retroviruses? How do they reproduce and what is the role of the enzyme reverse transcriptase? Retroviruses are viruses whose genetic material is RNA, HIV and the
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Which type of reproductive structure has a better chance of survival if there was a sudden temperature change in its environment? A.)asci B.)zygosporangium C.)basidium D.)lichen
Risk Characterization Risk Characterization : Integration of hazard identification, hazard characterization and exposure assessment into an estimation of the adverse e
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