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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Stress Testing after Myocardial Infraction? Many post infarction deaths are sudden and occur with in the first 6 months after infarction. Exercise testing from 10 days to we
Q. Explain Spoilage of Soft Drinks? We all consume soft drinks, fruit juices, as well as, preserves, which normally are available in the market as tetrapacks, cans, bottles
Pulmonary Regurgitation : Most of the clinical cases are after balloon valvotomy or surgical valvotomy using a trans annular patch. It can be quantified by Echocardiography and D
What happens to transcription at the lac operon when lactose is absent and why? What happens to transcription at the lac operon when lactose is present and why? What happens
- Nuclear membrane disorganize at the beginning of this phase. - Spindle fibers originate and the kinetochore fibers attach to the kinetochore of homologous chromosomes from eac
Cutaneous respiration in earthworm. The respiration performed by the skin is called cutaneous respiration In earthworm skin is the only organ of respiration. The skin
Inhibitor Proteins - Enzyme-activity Control One kind of inhibitor protein found in higher plants is an endopeptidase which degrades nitrate reductase thus causing irreversibl
long ans about types of ovules
Q. Is the epithelium vascularized? How do oxygen and nutrients reach the epithelium? Why is this feature an important evolutionary acquisition? Epithelia are not vascularized c
Vitamins Vitamins, as you may already know, derive its name from the word 'Vital Amine'. These are the organic compounds required for normal metabolism, growth and developme
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