Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

How alkaline cleaning compounds works, Q. How Alkaline cleaning compounds w...

Q. How Alkaline cleaning compounds works? Carbonates, bicarbonates, hydroxides of various metals are called alkaline compounds. Alkaline cleaning compounds have a pH between

In which type of animals does the placenta exist, In which type of animals ...

In which type of animals does the placenta exist? What is its main function? True placenta is present in placental mammals. The placenta is produced from the chorion of the

Plants - rapidly flowing waters, Plants - Rapidly Flowing Waters Among...

Plants - Rapidly Flowing Waters Among the plants only small, well attached forms, such as sessile algae can survive here. Thus, due to the presence of only a few plants, the n

Are the limbs modified into wings of bats, Are the limbs modified into wing...

Are the limbs modified into wings of bats and the wings of birds examples of evolutionary analogy or homology? What about whale fins compared to fish fins? Bat and bird wings h

Neo darwinism or synthetic theory of evolution, NEO DARWINISM OR SYNTHETIC ...

NEO DARWINISM OR SYNTHETIC THEORY OF EVOLUTION - It is the synthesis of the ideas of Neo Darwinians as a new theory of evolution based upon recent findings of genetics and m

A useful way to grow seeds, A useful way to grow seeds Tie a piece of c...

A useful way to grow seeds Tie a piece of cloth over the mouth of an old potted meat jar. Permit extra cloth to hang down the sides and dip in about 2 cm of water contained in

Conservation of biodiversity, Conservation of biodiversity is very importan...

Conservation of biodiversity is very important to maintain the ecological balance of ecosystem. Various steps for conservation of biodiversity: 1.           Creation of consc

Name the nitrogenous waste excreted in the larval, What benefit does the se...

What benefit does the sea anemone get in the sea anemone-hermit crab facultative mutualism? Give an alternative term for this part of mutualism. Name the nitrogenous wast

Calculate the electric potential at the midpoint of the base, The three cha...

The three charges in the figure below are at the vertices of an isosceles triangle. Let q = 7.00 nC and calculate the electric potential at the midpoint of the base.

Can you illustrate chylomicrons, Q. What is the special route that lipids f...

Q. What is the special route that lipids follow during digestion? What are chylomicrons? Triglycerides emulsified by the bile within micelles suffer the action of lipases that

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd