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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Class of Subphylum Uniramia - Symphyla Symphyla is yet other small myriapodous group that includes around 160 described species. These are also soil living forms and live in l
Q. What are zoonoses? What are few examples of zoonoses transmitted by birds? Zoonoses are human diseases transmitted by animals. Psittacosis, a bacterial disease, cryptococcos
What are heterochromatin and euchromatin? Chromatin is uncondensed nuclear DNA, the typical DNA morphology in interphase (the phase of the cell cycle in which the cells is not
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Conventional CABG on Cardiopulmonary Bypass Chest is opened by midline incision and median sternotomy. Simultaneously saphenous vein or radial artery is harvested. 'T
Q. Where does most of the water resorbed after glomerular filtration go? What are the other substances resorbed by the nephron tubules? Only 0.5 to 1% of the glomerular filtrat
Explain the histologic techniques A clinician has to base the diagnosis only on the clinical and radiographic findings as microbiologic and histologic techniques are restricte
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Explain Risk Assessment Risk Assessment : The scientific evaluation of known or potential adverse health effects resulting from human exposure to food borne hazards
What is the difference between an ecological niche and a habitat? An ecological niche is a set of peculiar activities, resources and methods that a species explores to survive
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