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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Which of the following best explains the reason why DNA ligase is needed to complete the replication of internal chromosomal segments? A. DNA ligase is able to delete the last
what is allometry?
Contig: Number of uses, all nouns. The term comes from the shortening of the word 'contiguous'. A 'contig' might refer to the map showing placement of a set of clones which comple
What is a stream? A stream is a incessant series of bytes that flow into or out of your program. Input and output from devices like the mouse, , disk, keyboard, screen, modem, a
LOCOMOTION IN STAR FISH - With the help of tube feet aided by fluid pressure in them. In a tube feet upper ampulla, middle podium and lower sucker present.
Isoniazid Serum aminotransferase activity increases in 10% to 20% of patients taking isoniazid, especially in the early weeks of treatment, but often returns to normal even wh
Unsaturated fatty acids Unsaturated fatty acids are liquid at room temperature and are characterized by having one or more double bonds. Fatty acids having two or more
You wish to use PCR to sequence a particular gene that you have isolated. The sequencing protocol suggests that you use 3.2pmole (pico moles) of your primer in a 2 micro liter volu
Suggest a mechanism that would allow a plant to grow better if it had intermorph neighbors than if it had intramorph neighbors. Think about the degree of genetic similarity between
Adenosine triphosphate (ATP) is a general form in which the energy is stored in living systems; comprises of a nucleotide (with ribose sugar) with three phosphate groups. Energy c
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