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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
State the similarity between Shigella and Salmonella There are many points of similarity between Shigella and Salmonella. They dwell primarily in the gastrointestinal tract, wi
BLOO D CORPUSCLES (= BLOOD CELLS) - Blood corpuscles are of the following three types: Erythrocytes, Leucocytes and Thrombocytes. (A ) Erythrocytes (Red Blood Corpusc
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Carrier ions like Na+ facilitate the absorption of substances like: 1. amino acids and glucose 2. glucose and fatty acids 3. fatty acids and glycerol 4. fructose and so
Ask question #M1. Discuss why Obelia is considered to be of special interest in Zoology as an animal showing an intermediate grade of organization. inimum 100 words accepted#
The F2 phenotypic ratio from a dihybrid cross with epistasis was 9:3:4. If instead of a dihybrid cross, a double heterozygote was test crossed (crossed to a homozygous recessive in
Define Sample Titration for Saponification Number of Fats? Measure 2 ml of oil with the help of a pipette and transfer this oil to a 250 ml conical flask. Add 40 ml of alcoholi
What are toxic effects of lectins or haemagglutinins? Well, these can cause growth inhibition in animals and diarrhoea, nausea, bloating and vomiting in case of human beings. W
Polysaccharide - polymer composed of monosaccharide monomers Starch - Energy storage in plants à straight (amylose) and branched (amylopectin) chains of α-glucose Glycogen
Haemoglobin Among the respiratory pigments we shall consider haemoglobin in some detail as this is the most familiar, widespread as well as the most efficient respiratory pigm
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