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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define the Concept of Public Nutrition? The rapidly changing global trends in the area of food consumption patterns, lifestyles and environment have a tremendous impact on the
G a ngrenous dermatitis (necrotic dermatitis) A bacterial condition in chickens caused by spores of Clostridium septicum , sometimes that of Cl. novyi / Cl. edematiens a
Q. What is the importance of iron in diet? What is the disease caused by iron deficiency? Iron acts as a constituent of the hemoglobin molecule and of enzymes of the energetic
Q. What is the difference between the reptile heart and the amphibian heart? The reptiles have incomplete and double circulation three heart chambers two atria and one ventricl
what does the sperm cell fuses with?
Consequences of Aging It is hard to enumerate the general consequences of aging in different animals. Gradual loss of reproductive ability, neuromuscular coordination and not
Physical Lay Out: Physical lay out of the nursing unit should be planned and built in such a manner so that it helps the head nurse to discharge her duties/carry out her resp
Food Chain - Ecosystem In a food chain, the food energy is transformed from a given source through a series of species, each of which eats the one before itself in the chain.
Blood from the fetus circulates through the placenta. a) What substances pass (i) from the maternal to the fetal blood, (ii) from the fetal to the maternal blood?
Q. Define Mushroom Poisoning? Mushroom poisoning is caused by the consumption of raw or cooked fruiting bodies (mushrooms, toadstools) of a number of species of higher fungl.
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