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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
does earthworm have rbc.
Q. Why is cannibalism an inharmonious intraspecific ecological interaction? In the cannibalism an individual eats other of the same species (occurs in some arachnids and insect
Bovine viral diarrhoea Bovine viral diarrhoea (BVD) and mucosal disease (MD) are clinically dissimilar disease syndrome yet have a common viral etiology. The acute disease is
Q. Explain about Low blood sugar? Low blood sugar can also be caused by accidentally taking too much of insulin. When this occurs, more insulin is present in the body than nee
Disposal : You must always sterilize cultures and contaminated instruments and glassware before disposing them of or before washing-up. Usually, you can do this by autoclaving or
Human Impact on Carbon Cycle Human activities have greatly influenced the carbon cycle. The discharge of CO 2 , into the atmosphere is steadily increasing owing to burning of
Q. What are carbohydrates? Carbohydrates are important organic compounds widely distributed in animals and plants. Plants can synthesize carbohydrates by the process of photos
Define Interaction of vitamin c with Ascorbic acid and Leucine? Ascorbic acid : The biologically active form, ascorbate, is a cofactor or co- substrate for eight isolated enz
describe the two scientific models for evolution (gradualism, punctuated equilibrium)
S a f e ty, ethical and welfare considerations Development of transgenic organisms is proceeding in an environment of intense public debate about safety, environmental impa
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