Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Animals, explain the modes of nurition in animals

explain the modes of nurition in animals

Describe in detail about the chlorophyll molecule, Describe in detail about...

Describe in detail about the chlorophyll molecule Each chlorophyll molecule contains magnesium ion, there could be no green plants without magnesium. As magnesium is present in

Explain regulation of water balance for cells in the kidneys, Explain Regul...

Explain Regulation of Water Balance for Cells in the kidneys? Cells in the kidneys respond to low blood pressure by releasing an enzyme called renin, which in turn causes the k

Energy input in ecosystem, Energy Input in Ecosystem Survival and func...

Energy Input in Ecosystem Survival and functioning of ecosystem is dependent on the input of energy. Continuous availability of energy is essential for supporting diverse ecos

Explain why the plasmid is described as a vector, Humans produce insulin fr...

Humans produce insulin from certain cells in the pancreas.The insulin gene is isolated from a human pancreas cell and then inserted into a plasmid.The DNA responsible for the synth

Methods of virus, How Viruses Multiply? Obligatory parasitism - Outsi...

How Viruses Multiply? Obligatory parasitism - Outside cells viruses are nonliving, inactive   particles but after entering into live cells these multiply fast by replication

Gases will be taken in and given out by a green plant, What gases will be t...

What gases will be taken in and given out by a green plant (a) in darkness, (b) in bright sunlight?   a) In low light intensities a green plant will be taking in oxygen and

What is the significance of pharyngeal gill slit, What is the significance ...

What is the significance of Pharyngeal gill slit ? Lateral opening in the wall of the pharynx which allows water to enter into the mouth and exit through the pharynx. This is on

What are dna ligases, What are DNA ligases? How do these enzymes participat...

What are DNA ligases? How do these enzymes participate in the recombinant DNA technology? The DNA ligases are enzymes specialized in tying the complementary DNA chains that for

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd