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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
The modern water treatment plan is often called upon to perform wonders with the water fed to it. The clear, safe , even tasteful water that comes from a faucet may have started a
Define the working of pH Meter? The pH of a solution, we know, measures the degree of acidity or alkalinity relative to the ionization of water sample. A pH meter is a sophisti
The Period of Persecution: Earlier. treatment of the inentally sick depended on men's various superstitious beliefs. It was thought that sounds and motions are the factors
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Q. Definition of Osseointegration in microscopic biology? From a view point of macro and microscopic biology and medicine. Osseointegration of a fixture in bone is defined as t
Cleft grafting The stem of the stock is cut across. A 'V' shaped insertion is made at the end of the stock. The scion is cut in shape. The scion is inserted into the 'V'
You are working at a large, well funded research institute with the latest biochemical techniques available. The focus of your research is to find a specific mutation, or set of mu
which intracellular organelles are present in bacteria?
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