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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are the uses of microorganisms
Given an individual who is heterozygous at 6 loci, how many different gametic Genotypes are possible? A. 36 B. 12 C. 32 D. 48 E. 64
mode of nutrition : out side the body
A fire hose ejects a stream of water at an angle of 38.8 ° above the horizontal. The water leaves the nozzle with a speed of 26.6 m/s. Assuming that the water behaves like a projec
Radioisotopes Radioisotopes are those forms of an element which exhibit rzdioactivity and so can be used as markers or tracers. For this purpose, we first introduce the radiois
Advantages (1) Liquid and gaseous fuels can be manufactured from solid fuels. (2) Relatively cheap and easily available. (3) These are easy to transport. (4) A
Determine the Occurrence of Vitamin E The tocopherols are widely found in animal and vegetable materials, like nuts and seeds. Considerable amounts are found in a number of
Define Supplementation to prevention of iron deficiency anaemia? Supplementation with low doses of iron is necessary to prevent anaemia in particular groups of people. Fortifie
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V AGIN A - Median, elastic, muscular tube 7.5 cm long. Open into vestibule by vaginal orffice. The lining forms vaginal rugae. Space between vaginal wall & cervix is forn
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