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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is Respiratory System explain their functionality ? The respiratory system is responsible for the transport of oxygen and carbon dioxide from outside the body to and from
Presenting Features Anterior abdominal wall and anterior bladder wall is deficient and on the surface everted posterior wall of bladder with two ureteric orifices is seen.
B o v i ne ephemeral fever Ephemeral fever is commonly known as 'three-day sickness'. It affects mainly cattle and occasionally sheep in India. Causative agent is a mosquit
what is primitive fungi?
Several TCA cycle intermediates can be used as a substrate for the synthesis of other compounds. Succinyl CoA is on intermediate that is directly used for the synthesis of which of
Define Objective of nutrient needs during periods of pregnancy? describe the various physiological changes during pregnancy, describe foetal growth and development and
Explain Photolithoautotrophs - Nutritional Types of Microorganisms? These microorganisms use light as an energy source, inorganic substance as electron source and CO 2 as the
Fracture - It is a break of a bone. Bone fracture are of many types - (i) Green stick fracture - It is merely a crack, the bone remains partly intact. (ii ) Sim
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What are the indications of implant removal? Failed implants need to be removed and the specific indications include: - Severe Perimplant bone loss (> 50 per cent implant le
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