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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
how they reproduce?
What are the characteristics of cardiac muscle that allow it to be highly resistant to fatifue?
Define Economic Evaluation of Malnutrition? You must be familiar with a proverb frequently used in the field of economics, which says - "resources are limited and wants are unl
Q. Diagnosis of diabetes mellitus? Timely and proper diagnosis plays a key role not only in identifying new cases but also in managing old cases with or without diabetic compli
R uminal tympany (Bloat) It is the over-distension of the rumen with gases which may be either frothy or free gas. The disease is characterized by change in silhouette of the
Characteristics Define Cancer - Hyperplasia Hyperplasia is the extreme proliferation of cells which can be observed in normal as well as cancerous tissues. In normal tissue, a
In a school laboratory, what is usually regarded as evidence that photosynthetis has occurred in a plant
write down the division of cryptogamae and phanerogamae
What are the near point and the far point of the vision? The near point is the closest distance among an object and the eye that makes possible the formed image to be focused,
1. (a) Which physicochemical properties need to be regulated by the cell? Define chemical equilibrium and discuss how it is maintained by the cell. (b) Differentiate between facili
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