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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Question 1 What is megalobastic anemia? Discuss briefly its lab diagnosis. How would you differentiate megaloblastic anemia from other anemias? Question 2 What is hemog
Question 1 : What do you meant by medical writing? Show various types of medical writing. Define and explain briefly medical writing Discuss various types of medical w
Brasseler Endo Extractor tube Insert H file buccally and lingually and remove silver by friction.If I can't remove use solvent to desolve cement If the tip of silver not appear
Define Tests to Measure Flexibility Component in the Humans? The flexibility component measures the elasticity or range of motion around a joint. This component of physical fi
Pollen Biology Fluorescence microscopy coupled with biochemical analysis has helped resolve the differential activity of the enzyme, β-l, 3- glucanase that catalyses the disso
Q. What do you understand by Chromosome Behaviour? When we study meiosis. we not only observe the regularity of pairing which is important' for' the fertility of the plants, we
Anterior Leaflet Procedure: Repair is more difficult and less successful. Previously, a triangular excision of the anterior leaflet used to be done for prolapse and rupture of
A typical chest x-ray exposes the patient to a radiation dose of 0.01 rem. If a man receives 10 such x-rays in a lifetime, what is his chance of developing cancer as a result of th
Natality Rate - Natality Natality rate or birth rate is determined by dividing the number of individuals born by unit time and is expressed as follows: Natality rate = ΔNn
what is the function of the germinative zone
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