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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
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Characteristics of Gametes Following are some general characteristics of gametes: 1. The female gametes are usually laden with food material, the yolk, while the male gamet
Consider a time when the membrane voltage of a SA node cell in the heart is at its minimum value (near -80 mv). At this time, A. all the voltage-gated calcium channels will be
Disorders of Thyroid Function: The disorders of thyroid function may lead to following problems: Juvenile Hypothyroidism Hypothyroidism is one of the most common end
Determine some Dietary Sources of Vitamin C? Fruits (particularly citrus fruits), vegetables are the best sources of ascorbic acid. Amla is the richest source of ascorbic acid.
Fensom and Spanner Electroosmotic Flow Hypothesis In electroosmosis the ions flow across a membrane in response to electrical gradient. Ions pull along water and other content
Handling : The desire for survival is same whether in animals or human beings. You may be aware of the ability of even tiny animals to scratch and bite. So we won't say any more o
Possible mechanisms of increased risk are that hyperhomocystinemia may impair release of nitric oxide form endothelial cells, stimulate proferation of atherogenic smooth muscle cel
important of biology to man
Q. Explain about Rheumatic Heart Disease? Rheumatic Heart Disease (RHD) is a very common cause of cardiovascular disorder in children and adolescents in India. This disease inv
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