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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Chemical bonding : The present matter of earth contains atoms bound in groups of two or more called molecules. Their binding capacity is called chemical reactivity; It depends upo
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What is the logic of the transfusional compatibility concerning the Rh blood group system? The Rh + donor can only donate blood to the Rh + recipient. The person that lacks t
Salinity - Marine Ecosystems Physico-chemical factors - Salinity The sea is salty and its salinity is fairly constant, averaging about 3.5 per cent usually written as
Peste des petits ruminants (PPR) is an acute, highly contagious viral disease of goats and sheep caused by peste des petits ruminants (PPR) virus which belongs to the genus Morbil
Chemotaxis - Modes of Cell Movement Chemotaxis considers to me directed movement of cells in response to a concentration gradient of a chemical factor in solution. An instance
Q. Can you explain Ventricular Septal Defect? VSD can be found in various part of ventricular septum hence all possible views except suprasternal views are used to detect an
Establishment of Suspension Cultures A callus crumbles into smaller clumps and single cells in liquid medium by gentle agitation (100-120 rPM) on a shaker. Shaking the cultur
How have prokaryotic cells given origin to aerobic eukaryotic cells and to photosynthetic aerobic eukaryotic cells? As per to the most accepted hypothesis aerobic eukaryotic ce
Define Mechanisms for Provide protection against colon cancer? Several plausable mechanisms have been formulated by which fibre may provide protection against colon cancer. The
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