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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are the differences b/w bone and cartiledge
Define the Quantitative Analysis in Nutritional Biochemistry? Quantitative analysis involves the measurement of the amount of a substance present. This measurement can be done
Q. Minerals requirements for dyslipidemia? Minerals: The three most important minerals are chromium, zinc and magnesium. These minerals play a critical role in maintaining p
What is aves?
Q. Show the Artificial Keys? These are based on the resemblances and differences of some prominent characters in the taxa. These are based on the phylogenetic relationship b
Explain Water as a lubricant? All fluids have lubricating as they can make it easier for the solid materials to slip over one another. Water-based fluids act as lubricants in
Define some adverse effects of saponins? The adverse effects of saponins may be described as under: - A well-known toxic effect is 'erythrocyte lysis' since they interact wi
In humans, maleness or femaleness is determined by a pair of sex chromosomes called X and Y. (a) What is the genotype for males? (b) What is the genot
evolution of metazoa
According to the Mendel's second law, in the crossing between homozygous individuals concerning two pairs of nonlinked alleles, AABB x aaBB, what are the phenotypical and genotypic
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