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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
There are two distinct methods through that cells synthesize ATP. In oxidative phosphorylation which containing the electron transport chain and the generation of ATP is linke
In many animals, glucose, rather than starch, is transported by the blood through the body to all the cells. Starches in many foods are digested to yield glucose. Why is the digest
Primary Succession - Community Change In primary succession on a terrestrial site the new site is first colonised by a few hardy pioneer species that are often microbes, liche
What is Coarctation of Aorta ? More common in males (3:l). Narrowing of aorta typically located near aortic attachment of ligamenturn arteriosum or PDA. It can be a localized
Define the genetic growth pattern of infant? It takes 1-3 years for an infant to establish his own genetic growth pattern. Once this figure is established (such as height-for-a
discuss about protozoa,metazoa,mesozoa and parazoa
Explain the Centrifuge - Microbiological Apparatus and Equipments? Centrifuge is used for separating the substance in liquid or concentrating the microorganisms on the basis of
What is metagenesis? What are the other names of this process? Metagenesis is the type of life cycle in which there are two dissimilar forms of individuals of the same species,
Explain Cyanotic Heart Disease breifly? Presence of cyanosis means deoxygenated blood reaching the systemic circulation bypassing the lungs i.e., right to left shunt. Uniform c
Define Diagnostic Criteria for Anorexia Nervosa? Table gives the American Psychiatric Association diagnostic criteria for anorexia nervosa. Although the manual does not use bod
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