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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. How Linnaeus classify the plant kingdom? Linnaeus classified the plant kingdom into 24 classes in his famous work 'Genera Plantarum' (1737) and 'Species Plantarum' (1753). I
Q. What are some major differences of the vertebrate nervous systems comparing to invertebrates? In vertebrates the nervous system is well-characterized, having dorsal neural c
The Peripheral membrane proteins are less strongly bound to the lipid bilayer than integral membrane proteins and can be readily erased through washing the membranes wi
What is the treatment of tuberculosis The disease can be very effectively treated with the help of antibiotic therapy, rest and nourishing food. The key to the treatment is ear
What are gonads? What are the male and the female gonads in humans? Gonads are the organs that make gametes. They have the germ cells that undergo division and generate gametes
SPLEEN Largest lymph gland, also with myeloid tissue is an important specialized reticuloendothelial organ in vertebrates, as the site of erythropoiesis. Splenic tissue i
ISOMERISM AND STERIOISOMERISM IN HEXOSE SUGERS
Q. Show the list of symptoms? The list of symptoms includes: • Bulky, pale, loose, greasy and foul smelling stools. • Anorexia, feeling of fullness, pain abdomen. The
EXAMPLES OF NATURAL SELECTION - 1 . INDUSTRIAL MELANISM - First studied by Fischer & Ford (1845) on pepperd moth (Biston betularia) of two types : grey moth (B.
Define Solvent Chemical Potentials from Phase Equilibria? Previously explained how we can evaluate the activity coefficient γ m,B of a nonelectrolyte solute of a binary soluti
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