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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Solutes and ions tie up water in solution. Solutes lower aw and this reduction in aw depends on the total concentration of dissolved molecules and ions. Since these bind to water m
Critical Thinking Why is it necessary to use the same restriction enzyme to cut two pieces of DNA that are to be joined together? As each restriction enzyme recognizes a specif
In which meiotic division does the separation of the homologous occur? What are the ploidies of the generated cells after the end of that process? The separation of the homolog
Or nit h o s i s (psittacosis) This is an important zoonotic bacterial infection and causes disease in humans and birds. Collectively, these conditions are called as chla
Q. Can you explain Ventricular Septal Defect? VSD can be found in various part of ventricular septum hence all possible views except suprasternal views are used to detect an
Describe Lifetime Risk Estimation ? Traditional statistical and epidemiological methods of estimating long-term risks do not accurately reflect population burden of disease. Th
Define effect on Human placental lactogen in pregnancy? Human placental lactogen, with a structure similar to the growth hormone, increases throughout pregnancy. Its rate of pr
Aging population is an important factor to be considered in implant dentistry. Life expectancy has increased significantly past the age of retirement. Social pleasures continue thr
Define the term - innor mitochondrial membrane The innor mitochondrial membrane has about 60 different proteins associated with it. The outer membrane is associated with some
Define nutritional management of severe anorexia nervosa? The nutritional management of severe anorexia nervosa is therefore, considered in terms of three consecutive phases:
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