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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Generally which phase of the cell cycle has longer duration? The mitotic phase has quite a shorter length and the interphase comprises approximately 4/5 of the cell cycle.
Q. What are the four initial stages of the embryonic development? The four early stages of the embryonic development are the blastula stage, the morula stage, the gastrula stag
Define Molisch's Test or alpha naphthol reaction? The Molisch test is a common test for the existence of carbohydrates. Principle The reaction is due to the formation of
As bone looses width, height, volume, the attached gingiva gradually decreases and is prone to abrasions caused by the overlying prosthesis. In addition high muscle attachments and
Consumers - Biotic Components These are also called as phagotrophs or heterotrophs. The organisms grouped under this category cannot manufacture their own food but obtain the
Prophylaxis High-risk neutropenic patients, such as those undergoing allogeneic and certain autologous stem cell transplants, and those with hematologic malignancy who are exp
Identify and briefly explain the structural and functional changes that occur in the large bowel when colorectal cancer develops.
Diplotene: The paired chromosomes repel each other and begin to separate. Separation however, is not completed, because homologous chromosomes remain united by their point
write the raspiration of different types of animals of different phylum
Explain in brief about the term - Calcium Calcium is absorbed as Ca 2+ ion and is a constituent of cell wall, an activator of different plant enzymes and is essential for t
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