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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Describe Class Holothuroidea in details? Members of this Class resemble soft and squishy cucumbers lying on their sides on the bottom of the sea. On first glance, they appear n
Define Functions of Carbohydrate - Source of energy? Glucose is a major source of energy for all the body cells. One gram of carbohydrate provides 4 Kcal. RBCs are particularly
Hydrophily - Cross-pollination All hydrophytes are not necessarily pollinated by water. In fact most of the aquatic plant are anemophilous, e.g. Alisma, Nymphaea. Like anemoph
Preparation on the Previous Day of Operation Remove all the jewellery, nail polish, cut nails. The patient's body is cleaned - shaving from chin to toe, inclu
A flashlight with a 6-volt bulb draws 2 amps from its batteries. What is the resistance of its circuitry?
Minerals:- Selenium Food Source Sea food, organ meats, cereals (levels vary depending on soil levels) Nutritional Functional role Essential nutrient: Keshan
Explain the role of cuvier in animal taxonomy? Cuvier (1769-1832) was critical of Lamarck's evolutionary concept which thereafter remained in oblivion for half a century. Cuvi
THE DENVER DEVELOPMENT SCREENING TEST (DDST) The Denver Development Screening Test is a standardized test to assess gross motor, fine motor, language and social development
Explain the Features of Alternaria? 1. Alternaria colony is wooly and compact. Underside is very dark coloured. Colony colour is grayish green or black with gray edges rapidly
WRITE ON FUNGAL NUTRITION
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