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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Micronutrient needs of a lactating mother? Vitamin and mineral deficiencies can have profound influence on the composition of milk. Calcium is a nutrient of special conc
In this section you will be introduced to a variant of Darwinian concept of natural selection which he termed sexual selection. You may have observed very distinct and striking dif
Blastula is the ball of cells surrounding a fluid-filled cavity (known as blastocoel) which is produced by the cleavage of a zygote.
Which of the following is not needed for C to U deamination during RNA editing? A. Formation of localized segments of double stranded RNA. B. The 5' and 3' efficiency elemen
Define Complete Assessment for Dietary Management during Surgery? A complete assessment must include: Physical examination (anthropornetric measurements such as ideal/us
Q. Explain Femoral Approach? This approach involves the insertion of a catheter over a guidewire i.e. inserted into a sheath in the right femoral artery. Systemic anticoagulati
SIGNIFICANCE OF FERTILIZATION - 1. In fertilization process number of chromosome are diploid in zygote. 2. During fertilization male & female chromosomes are combi
Nutrition in amoeba?
FLUI D MOSAIC MODEL Proposed by Nicholson and Singer (1972) Proteins and lipids are arranged in a mosaic fashion. Lipids and proteins are in a semi fluid structure.
Q. Definition of Biological diversity? Biological diversity has no single standard definition. One definition holds that biological diversity is a measure of the relative diver
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