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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are the similarities between chlorplasts and prokaryotic cells?
define analogous
Explain about the Adolescent Pregnancy? The pregnant adolescent is viewed as a high-risk patient, highly susceptible to suboptimal pregnancy outcome. Even when not pregnant, a
Some activities that make use of biological resources, without involving their extraction or destruction, denote non- extractive use of biological resources. This includes ecotouri
Q. How you would dispose off sharps and needles? Used needles should not be bent or broken prior to disposal. Unsheathed needles are placed directly into the designated, punctu
What are neurodegenerative diseases and how might they be treated? Neurodegenerative diseases that include Alzheimer's, Parkinson's, Huntington's, and amyotrophic lateral scler
The plasmids below show the insertion of the EZH2 gene into the pBluescript plasmid in either sense (forward) or antisense (reverse) orientation. These are the plasmids you will ho
What is the condition for Mendel's second law to be valid? Mendel's second law is only valid for genes located in dissimilar chromosomes. For genes situated in the same chromos
What is the endocrine function of the placenta? The placenta is not a permanent gland of the endocrine system but it also has endocrinal function. The placenta produces estroge
Effects of Kwashiorkor on children Kwashiorker is an African word- it means displaced child. The child is weaned out very early and is given foods having high amounts of carboh
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