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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the role of dietitian's in feeding dilemmas Usually, the dietitian's role in feeding dilemmas is seen as secondary and the physician's role is the focus. In reality,
Explain about the Spectrophotometer? The spectrophotometer, a key instrument today in biomedical laboratories, was invented in 1939 by the American chemist Arnold O. Beckman (1
Technique of food processing Freezing is another ancient technique of food processing, which allows the transportation of perishable food items for long distances from producti
Why should you not apply cosmetics while doing a lab experiment in class?
Compared to amphibians what are the two reproductive novelties of beings of the class Reptilia for the survival in dry environments? Compared to amphibians the two major reprod
Q. Which arthropod class is the mainly diversified animal group of the planet? How can this evolutionary success be explained? The insects are the animal group with most divers
Q. Does each amino acid have a central carbon? To which organic group is that central carbon bound? A carboxyl group -NH 2 , an atom of hydrogen - COOH, an amine group-H and a
Food Applications of hemicelluloses The hemicelluloses find their application in food systems as emulsifer, stabilizer and binder in flavor bases, dressings and pudding mixes.
Define Chromatography - Basic Separation Technique? Chromatography is the most effective technique for the separation, purification and identification of one or more biological
Q. How do cells of neoplastic tumors obtain oxygen and nutrients and release wastes? In the neoplastic tumors a phenomenon called as angiogenesis occurs. The Angiogenesis is th
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