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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Indifferences in ionic composition Answer A. across membranes can be created through the action of ATP driven pumps as long as number of negative and positive ions on remain equal
How to increase in the amount of glucokinase in liver Consumption of a meal rich in carbohydrate or administration of insulin initiate an increase in the amount of glucokin
Biological Nitrogen-Fixation The process by which molecular nitrogen (N 2 ) is reduced to ammonia (NH 3 ) is called nitrogen-fixation (N 2 -fixation). This is the most importa
What is the life cycle of the hookworms? Adult hookworms within the human intestine release eggs that are eliminated with the human feces. Under adequate conditions of moisture
economic importance of mollusca
Explain the Bioavailability of Vitamin D? The nutritional availability of vitamin D is less significant because it can be endogenously produced and retained for long periods by
What is critical photoperiod? And How can the critical photoperiod relate to flowering be experimentally determined? Critical photoperiod is the limit of the photoperiod durati
can you tell 10 examples of trinomial system of nomenclature?
Advise on Rest, recreation, sleep The patient should take enough rest and get good sleep. Restrict the visitors so that the patient can get enough rest. Not to strain th
Explain the Difficulty of establishing and sustaining collaborations? Working with another is both rewarding and challenging. Working across disciplines is even more so. Though
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