Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What do you mean by punch cards, Q. What do you mean by Punch cards? Pu...

Q. What do you mean by Punch cards? Punch cards are used when the number of taxa to be keyed out is large. In this type of cards the holes at numbers corresponding to a charact

Ethylene - dormancy, Ethylene - Dormancy Ripening of fruits involves a...

Ethylene - Dormancy Ripening of fruits involves a chain of cellular events like: Rise in the rate of respiration, Breakdown of higher carbohydrates into carboxylic

Transport in phloem, Transport in Phloem The basic necessities of plan...

Transport in Phloem The basic necessities of plants, water, are taken up by the roots. Another purpose served by the roots is to absorb water soluble mineral nutrients from th

Respiration in fishes, RESPIR A TIO N IN FISHES - Respiratory org...

RESPIR A TIO N IN FISHES - Respiratory organs are gills. In elasmobranchi 5-7 pairs of gills present, spiracles present. In Teleostomai 4 pairs gills present, covered

Explain the predominantly diurnal, Evidence  Similarities between 79° a...

Evidence  Similarities between 79° and 70°, eg Feeding activity continuous / continues for 24 hours during polar day (summer months) feeding activity more intense i

Osteogenesis - production of bone on soft tissue, Osteogenesis: Production ...

Osteogenesis: Production of Bone on Soft Tissue Now that we know the players in bone adaptation, we will look at the ways in which bone may be created and modified. There are t

Effects of smoking on oesophagitis, Q. Effects of smoking on oesophagitis? ...

Q. Effects of smoking on oesophagitis? 1. A decrease in pyloric sphincter pressure which may predispose an individual to duodenal ulcer. 2. An alteration in the nature of ga

Describe how platelets interact to form a blood clot, Describe briefly how...

Describe briefly how platelets, fibrin and red cells interact to form a blood clot.   Platelets release a substance which, indirectly, causes fibrinogen to be changed to fi

Newcastle disease / ranikhet disease (nd/rd), N e wcastle disease / Ranik...

N e wcastle disease / Ranikhet disease (ND/RD) This disease is caused by Newcastle disease virus (NDV) belonging to the genus Paramyxovirus of the family Paramyxoviridae. Alo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd