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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
The Word 'disaster' is believed to be originating from the French word 'Disastre' which is a combination of two words 'des' meaning bad and 'astre' meaning star. Thus this term ref
how respiration takes place in mammls and reptiles
What is Binary Fission in cell reproduction? Cell division takes place in prokaryotic cells by binary fission, also called prokaryotic fission. In prokaryotes, DNA is contained
Types of Biogeochemical Cycles There are two basic types of biogeochemical cycles, gaseous and sedimentary. In the gaseous type of biogeochemical cycle there is a prominent ga
Explain about the Toxicity of pyridoxine? Though toxicity related to pyridoxine intake are rare, but use of high doses of pyridoxine for the treatment of pre-menstrual syndrom
Q. What is Parkinson's disease? The Parkinson's disease is a degenerative disease of the nervous system in which the major manifestations are progressive motor disturbances, li
Potassium (K) - Macronutrients The main source of K + for plants comes from withering of K containing minerals. Potassium released by withering dissolves in the soil solution
General Characteristics of Protozoans The protozoans, are eukaryotes that form a group of about 80,000 single-celled organisms under the kingdom Protista. The unicellular leve
What are some things that have algae in them? Yeast is considered Algae.
GENERA L MECHANISM OF BLOOD COAGULATION - All researchers agree that blood clotting is a cascade mechanism and involves three essential steps - (a) Formation of prothrombin ac
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