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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Determine by nutritive muscular cell? The cells which form the gastrodermis lining the inner cavity of cnidarians. They carry out two functions: First is to absorb and digest f
Vitamin E (DL-α-Tocopherol) DL-α-Tocopherol is a yellow to red-brown, clear, viscous oil almost without odour which decolourise when exposed to light and air. DL-α-Tocopherol
Name the two hormones produced by the pancreas and say (a) in what circumstances, (b) in what way, they adjust the glucose concentration in the blood.
What are the three main types of trophic pyramids studied in Ecology? The three kinds of trophic pyramids studied in Ecology are the numeric pyramid, the biomass pyramid and th
Grasping instruments -Plier or clamp forceps -put piece of rubber in the plier -applied on the buccal and lingual surface -make pressure to
he recombinant plasmid extracted from the surviving colonies contains a gene called SUC2 that codes for convertase, an enzyme needed to metabolize sucrose. Mutations in the SNF gen
What is the mode of nutrition in fish,human,amoeba,scorpian & toad
Explain the Formation of enzyme? Formation of enzyme: Vitamin D is essential for the formation of two enzymes - alkaline phosphtase in the intestinal lining (involved in cal
What is resting membrane potential of a neuron? What is the function of sodium potassium pumps in maintaining it?
Define requirements of Vitamin A of infants? On the basis of vitamin A ingested by breast fed infants in well-nourished communities, ICMR (1990) has recommended a daily allowan
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