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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
We have no antibodies against any blood group when we are born still we say that some blood groups are incompatible for us.why?
Which of the below terms laws best describes the statement: Members of a homologous (pron: ho-MOL-eh-gus) pair of genes are separated at the time of meiosis (pron: my-O-sis) o
Define Types of Indicators? Macro indicators are used at strategic levels while micro indicators are used at performance levels. From the previous sections it is clear that man
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Nursing Assessment If the cause is due to hypovolemia you may observe the child for dry mucus membrane, poor skin turger, hypotension and oliguria. You should also observe
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what are characteristics of protozoa?
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