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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What are the general functions of the connective tissues? The major functions of the connective tissues are: filling and supporting of spaces, cellular nutrition, energetic
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Respiration in animals
Width of Attached Gingiva at Implant Site The zone of attached gingiva needs to be intact and of adequate width to protect (by separating) the tissue around the implant. If it
Q. Explain the Technique of Balloon Mitral Valvuloplasty? The procedure is performed by cannulation of the right femoral vein and the procedure is similar upto transseptal punc
Enterocoelous pattern – Coelom In enterocoelous development of coelom the mesoderm arises in the embryo as paired lateral pouches growing out from the archenteron. The pouche
Regulation of Respiration Whenever the need for oxygen in the body increases, ventilation of the respiratory organs also increases. In the same way whenever the level of oxyge
What is Post Myocardial Infarction Ventricular Septal Defect ? Post Myocardial Infarction Ventricular Septal Defect (Septal Rupture) The correct terminology is septal
What is a single cell protein? The proteins obtained from microbial sources, i.e. algae, fungi, bacteria, yeast etc. are referred to as Single Cell Proteins. These are isolated
Q. What are the extraembryonic membranes present in vertebrates? The extraembryonic membranes that may be present in vertebrates are the yolk sac, the amnion, the placenta, the
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