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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Why in the preparation of a karyotype analysis is the use of a substance like colchicine interesting? Colchicine is a substance that disallows the formation of microtubules and
Species diversity An important attribute of a community is its species diversity. The diversity is calculated both by the number of species (richness) and the relative abundanc
Mylohyoid muscle Surgical manipulation at the crest of a severely resorbed ridge may injure the mylohyoid muscle. Manipulation of the tissues of the floor of the mouth for plac
Most of the surface water originates directly from precipitation in the form of rainfall or snow. Ground water from springs also contributes to the flow of most of the streams. The
classes of phylum porifera
Explain the Digestive System ? The digestive system takes in food and processes it. Digestion is the mechanical and chemical breaking down of food into a form that can be used
Photosynthesis - Responses of Plants to Stress Photosynthesis is also inhibited due to low water potential in the leaves. The leaf water potential is the sum of turgor pressur
It is a neural mechanism that plays a role in encoding of new information. Encoding takes place as a result of changes in the strength of synapses among neurons in neuronal network
Plot the amount of DNA in the nucleus of spermatogonia from the G1 stage prior to the first meiotic division through the completion of meiosis. Label each of the major stages of th
Stratification On the basis of the variation in air temperature. the atmosphere has been divided vertically into four layers: (figure shown below) thee troposphere, stratospG,
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