Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

How are living beings divided into two groups, Q. According to the cellular...

Q. According to the cellular organization how are living beings divided into two groups? Cellular beings are divided into two groups, unicellular beings and the prokaryotes who

Express the monomer unit of a protein, Describe the monomer unit of a prote...

Describe the monomer unit of a protein and how monomer units are assembled into peptides.

Composting, C o m p o s t i n g It is a modification of a conv...

C o m p o s t i n g It is a modification of a conventional or solid manure handling system with anaerobic decomposition. Types of composting systems include windrow, b

Hydropericardium-hepatitis syndrome, H yd r o p e r i c a r d i...

H yd r o p e r i c a r d i um - h e pa titi s syndrome Also known as 'Angara disease' or 'leachy heart disease', this complex illness of poultry spread rapid

Phases of water, Phases of Water The state of water can be changed from...

Phases of Water The state of water can be changed from gaseous to liquid and liquid to solid and vice versa by the addition or removal of heat energy. To convert one gram of li

What is adaptive convergence?, What is adaptive convergence? The Adapti...

What is adaptive convergence? The Adaptive convergence is the phenomenon by which living beings facing the same environmental pressure (problems) and undergoing genetic variabi

Nucleus, NUCLEUS First described and christened by Robert brown 1831, a...

NUCLEUS First described and christened by Robert brown 1831, a nucleus is the largest and most important organelle of eukaryotic cells. It is membrane bound and generally locat

Regulation of the renal function, Q. Which are the three hormones that part...

Q. Which are the three hormones that participate in the regulation of the renal function? Antidiuretic hormone or ADH or vasopressin, atrial natriuretic factor (or ANF) and ald

Genetics Problems, Andalusian chickens may be either black, white, or gray....

Andalusian chickens may be either black, white, or gray. The gene for black is not dominant over the gene for white, nor is the gene for white dominant over the gene for black. Whe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd