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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
The common nitrogen-fixer in paddy fields is: 1. Rhizobium 2. Azospirillum 3. Oscillatoria 4. Frankia Azospirillum is the common nitrogen-fixer in paddy fields
What are lateral meristems? Where can they be found and which type of plant growth do they promote? Lateral, or secondary, meristems, are the cambium and the phellogen (also ca
A detail note on cryptophyes
DNA (deoxyribonucleic acid) is the molecule which are responsible for storing and transmitting the genetic information. DNA is a double- stranded molecule which is held together b
Q. What is self-catalytic RNAs? Ribozymes - Term 'ribozyme' was initially suggested by Thomas R. Cech, Nobel Prize winning biochemist, who discovered this class of RNA molecul
What are hydrophobic molecules (or hydrophobic molecular regions)? What are hydrophilic molecules? How can they be characterized in relation to their polarity? Hydrophobic mol
Petrol or gasoline can be synthesized by following three methods: 1. Polymerisation 2. Fischer - tropsch method 3. Bergius process polymerisation In
Explain Methodology Required for Osazone Test? Take about 250-300 mg of phenylhydrazine mixture in the 3 clean labelled 15 ml dry test tubes. Add 5 ml of the glucose, fructose
Estimation of protein by Miscellaneous parameters include? Miscellaneous parameters include Plasma amino acid levels Microbiological methods Chemical scoring
What is Metallic implants Metallic implants undergo one or more several surface modifications to enable them to become suitable for implantation. These modifications are passiv
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