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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How does the sexual arousal mechanism in women facilitate fecundation? During sexual arousal in women the vagina secretes substances to neutralize its acidity therefore allowin
Q. What is the kind of digestion that occurs in cnidarians? These animals have a digestive cavity and they make intracellular and extracellular digestion. The extracellular dig
Q. Do echinoderms present external or internal fecundation? Is there sex division among individuals? The fecundation in echinoderms is external, gametes are liberated in water
Biological mechanisms Annual rhythm. Innate behaviour pattern stimulated by environmental cues (shortened day length is the cue for preparation - candidates should exp
Hypothesis: The spelling of hypothesis when written as "hypotheses" indicate plural. The hypothesis is a statement or declaration of the expected outcome of a research st
Hipot is an abbreviation for high potential. Usually, Hipot is a term given to a class of electrical safety testing instruments used to confirm electrical insulation in finished ap
I am studying the evolution of a giraffe. How do I find more information?
How VIBRIO PARAHAEMOLYTICUS spread in humans Associated foods:- Vehicle foods for outbreak are raw, improperly cooked, or cooked recontaminated seafoods, such as, oysters, s
Hemoconcentration Before, during and after CPB, hemoconcentration allows the patient's blood to be salvaged during surgery to help reduce the need for donor blood. Ultra
Alternative polyadenylation sites Exact pre-mRNAs hold more than single group of signal sequences for 3' end polyadenylation and cleavage. In some cases, the area of t
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