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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Which is the type of nitrogen waste eliminated by beings of the class Reptilia? These beings excrete mainly uric acid. This substance is less toxic than ammonia and it can be k
What is the Importance of vitamin B 6 In the human and animal organisms, vitamin B 6 acts in the form of pyridoxal. In the form of 5'-orthophosphate (Pyridoxal-5'-phosphate),
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Pulmonary valve is surface marked at the sternal end of the left 3rd costal cartilage. Aortic valve is surface marked at the sternal margin of the left 3rd intercostal space. Mitra
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Explain the Flavour Binding? Some protein preparations, although acceptable from a functional and nutritional stand point, necessitate a deodorizing step to remove the bound of
what is ecology?sub divison of ecology?
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