Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Immunity, We have no antibodies against any blood group when we are born st...

We have no antibodies against any blood group when we are born still we say that some blood groups are incompatible for us.why?

Explain law of segregation, Which of the below terms laws best describes th...

Which of the below terms laws best describes the statement: Members of a homologous (pron: ho-MOL-eh-gus) pair of genes are separated at the time of meiosis (pron: my-O-sis) o

Define types of indicators, Define Types of Indicators? Macro indicator...

Define Types of Indicators? Macro indicators are used at strategic levels while micro indicators are used at performance levels. From the previous sections it is clear that man

How to increase in the amount of glucokinase in liver, How to increase in ...

How to increase in  the amount of glucokinase in liver Consumption of a meal rich in carbohydrate  or administration  of insulin initiate an increase in  the amount of glucokin

Chlamydiosis-pathogenesis, Pathogenesis The mode of intracellular repl...

Pathogenesis The mode of intracellular replication of chlamydial agents is investigated morphologically and cytochemically with both light and electron microscope. It is estab

Nursing assessment of acute renal failure, Nursing Assessment If the ...

Nursing Assessment If the cause is due to hypovolemia you may observe the child for dry mucus membrane, poor skin turger, hypotension and oliguria. You should also observe

Where is the gall bladder located, Where is the gall bladder located? T...

Where is the gall bladder located? The gall bladder is located in the Upper Right Quadrant (URQ) of the abdomen just below the liver. It keeps bile secreted by the liver. The d

Explain about gout disease, Q. Explain about Gout disease? Gout is a ch...

Q. Explain about Gout disease? Gout is a chronic disease due to an inherited abnormality of purine metabolism. What are purines? You may recall studying about purines and their

What is viscosity?, Q. What is viscosity? Viscosity is resistance to fl...

Q. What is viscosity? Viscosity is resistance to flow of a liquid. It is a measure of the resistance of a fluid to deformation under shear stress. It is commonly perceived as "

Protozoa, what are characteristics of protozoa?

what are characteristics of protozoa?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd