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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is the type of digestion that occurs in cnidarians? These animals have a digestive cavity and they make intracellular and extracellular digestion. The extracellular digest
what SOAP is: Subjective - The data includes information obtained from the patient or the patient's family regarding the problem. Objective dat a - the data is gathered f
Q. Where is it produced and what is the function of secretin in the digestive process? Secretin is made in the duodenum the chyme acidity causes the duodenum to release this ho
process of excretion in reptiles
You have 875 mL of a 0.37 M acetic acid solution. What volume (V) of 1.20 M NaOH solution must you add in order to prepare an acetate buffer of pH = 4.36? (The pKa of acetic acid i
Explain Indications for Surgery of Congenital Pulmonary Stenosis ? Interventional treatment is required for all neonates presenting with critical pulmonary valvar stenosis. Thi
Q. How chloroplasts multiply? Like mitochondria chloroplasts have their own the DNA, ribosomes and RNA and they self-replicate through binary division.
Q. What are the two main species of taenias that cause human diseases? The two chief species of taenias that cause disease in humans are Taenia solium, or the pork tapeworm, an
Define the process of Dough Formation? You already know that gluten is the protein found in wheat. A unique property of gluten proteins of wheat grain endosperm (and to a lesse
Anticodon is the series of three nucleotides on the transfer RNA molecule which recognizes and pairs with the specific codon on a messenger RNA molecule; it helps in controlling t
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