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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Explain Atherosclerosis? Atherosclerosis is one of the most important causes of morbidity and mortality in developed as well as in developing countries. Atherogenesis occurs
Trophic structure Organisms in a community are closely interrelated with each other through feeding relationships. Another aspect which is quite obvious in a community is th
What is Fermentation ? Fermentation is a process used by anaerobic organisms and certain cells of aerobic organisms, such as muscle cells deprived of oxygen, to obtain addition
MYCOPLASMOSIS Mycoplasma organisms are the smallest free living, lack of cell-wall and bounded by a triple layer of plasma membrane. The organisms are pleuomorphic in shape, viz.
I have 9 questions in my undergraduate cardiovascular physiology class I need help with. It is mostly dealing with slow response action. I need the work in about 2nite hours from n
Define the Reaction to Food Additives? Food additives such as preservatives, flavour enhancers and colouring agents have been linked to adverse food reactions. Additives implic
Are the phloem and the xylem made of living cells? The cells of phloem are living cells and the cells that constitute the xylem ducts are dead cells killed by the lignin deposi
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CHARACTERISTICS OF FEMALE'S SKELETON - 1. Skull is lighter. 2. Shoulders are nanow. 3. Sacrum is shorter but wider. 4. Pelvis is wider. 5.
In which chloroplast structure are chlorophyll molecules found? Chlorophyll molecules are placed in an organized manner in order to increase the exposure to light on the thylak
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