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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Others Factors Affecting Occupational Health The list of factors which affect the occupational health and that need standard organising, scrutiny, inspection, surveillance and
Q. What is the life cycle of a tapeworm? The Pregnant proglottids with taenia eggs are released together with human feces. If ingested by the intermediate hosts, bovineor swine
Describe the uses of pupil Variation in the size, shape or reaction usually indicates some form of disease, disorder or use of medication. The pupil is usually dilated by the u
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Pressure and Continuity Pressure Water pressure increases with depth which varies in the ocean from 1 atmosphere where at the surface to 1000 atmosphere at the greatest
how is biotechnology useful?
An organism having the genotype AaBbCcDdEe is self-fertilized. What are the proportions of gametes that are expected to carry only dominant alleles?
Mechanism of Stomatal Opening It has been known for over a century that stomata open because of reversible turgor changes in the guard cells. Stomata open when turgor in the g
Explain the Voges Proskauer (VP) Test? As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly conve
Pyramid of Biomass - Ecological Pyramids The pyramid of numbers is not a very good method to use if the organisms at different trophic levels are of greatly differing sizes. T
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