Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Explain functional properties of food lipids, Functional properties of food...

Functional properties of food lipids Chemically, fats and oils, as you may already know by now, are a combination of glycerol and fatty acids. The glycerol molecule has three s

What would be the flow rate, The physician orders 2 liters of lactated ring...

The physician orders 2 liters of lactated ringers over 16 hours. The drip factor of the tubing is 20 gtt/cc. What will be the flow rate? a. 33.33 or 34 gtt/min b. 41.66 or 42

Animals vs plants, Animals vs Plants Organisms are of two main types an...

Animals vs Plants Organisms are of two main types animals and plants, although all the above mentioned Unifying concepts of Biology apply equally to both animals and plants, ye

Explain food applications of xanthan, Explain Food Applications of xanthan ...

Explain Food Applications of xanthan Xanthan gum is mainly considered to be non-gelling and used for the control of viscosity due to the tenuous associations, endowing it w

Bovine rotavirus diarrhoea, Bovine rotavirus diarrhoea The bovine rota...

Bovine rotavirus diarrhoea The bovine rotavirus is a RNA virus with 11 segments of double stranded RNA belonging to the genus Rotavirus in the family Reoviridae. Rotaviruses c

Explain the regulation of blood glucose concentration, Explain the Regulati...

Explain the Regulation of Blood Glucose Concentration? A number of mechanisms function to maintain blood glucose at remarkably constant level of 70-100 mg/dl under fasting cond

Common features of eye and limb development, Common Features of Eye and Lim...

Common Features of Eye and Limb Development A comparison of the embryonic development of the very much different organs, the eyes and the limb, points to various interesting

Which are the organs of the excretory system, Q. Which are the organs of th...

Q. Which are the organs of the excretory system? The excretory system is formed of ureters (two), kidneys (two), bladder and urethra.

Population growth - population parameters, Population Growth - Population P...

Population Growth - Population Parameters and Regulation The size of a population depends upon the balance between natality and immigration through which individuals are added

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd