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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Which of the following mediates the interactions among the splicing machinery and the mRNA transcript? A. Alpha helical structures within the RNA binding domain of the splicing
Kingdom Animalia Multicellular, heterotrophic, eukaryotes, tissues are specialised and most of them have organs, mostly highly responsive. Only gametes are haploid, fertilisation
Q. Can you define termination of the stress Test? Test should be terminated when: 1) PVCS develop in pairs or with increasing frequency or when ventricular tachycardia devel
Similarities between autotrophic succession and heterotrophic succession
What is Metabolism ? The sum total of vital activities ( movements , nutrition, growth, respiration, excretion , reproduction, behaviour ,etc. Is the life of a living bei
Illustrate Nernst equilibrium potential for potassium ions A complete motor neuron is removed from a frog and placed in normal physiological saline at 1 AM. The neuron is hea
Q. What is severity of the oesophagitis? The severity of the oesophagitis resulting from oesophageal reflux is determined by the content of gastric reflux mucosal resistance,
assignment
Blood functions to maintain homeostasis in the human body through all but which of the following: Answer moving carbon dioxide away from cells following completion of aerobic metab
P ARTURITIO N - Gestation period is of 280 days (in rabbit 28 days). Parturition is the expelling of fully mature young ones from the mother's uterus. Oxytocin stimulates
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