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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Insulin binding to insulin receptors in the plasma membrane of a A. liver cell will lead to an enhance in the intracellular amounts of cAMP in the liver cell. B. beta-islet
Define the different causes of under nutrition ? 1. Scales (Screening Tool): The basic biochemical and anthropometric parameters are used in this screening. 2. Recognizing th
MOVEMENT OF BODY PARTS - Movement of appendages bring about locomotion. Perapodia, setae in Annelida, jointed legs in Arthropoda, flat foot in mollusca, tube feat in sta
What is the function of vitamin E? In which foods can it be found? Vitamin E, or tocopherol, is a fat-soluble vitamin that participates as coenzyme in the respiratory chain, t
A large number of integral proteins in eukaryotes do not traverse the membrane but are anchored in one or other leaflet of the bilayer through covalent attachment to a hydrocarbon
What is the Harshey-chase experiement .explain in detail
what features are in a epithelial cell
Q. What are the major theoretical models that try to explain the formation of the enzyme-substrate complex? There are two major models that explain the formation of the enzyme-
What is the Axes of Fick The Axes of Fick show that any eye position can be specified as the amount of rotation around a set of axes fixed in the orbit: 1) Vertical Axis: Z-
What is Translocation? Sometimes pieces of two chromosomes that are not homologous break off at the same time. If the broken pieces are re-attached to the opposite (in this cas
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