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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Precautions for Isolation of Pure Culture? 1. Plating of the medium should be done 24 hours before performing the streaking. There should not be any moisture on the
What is Procambium? The procambium produces cells that eventually become the vascular tissues xylem and phloem. These primary vascular tissues form a core in the center of th
How do white cells differ from red cells (a) In their structure, (b) Their function? a) White cells have nuclei, red cells do not have nucle
What is the main moral problem about the cloning of human individuals? As well biological perils, a very serious moral problem involves the nucleus transplantation technology c
Pantothenic acid (Calcium pantothenate) Calcium pantothenate is a white, loose, faintly hygroscopic powder without odour and of bitter taste. It is easily soluble in water, gly
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Determine the Preservation Changes While Cooking? While cooking, two preservation changes (at least) occur which include: 1. Destruction or reduction of microorganisms 2
Define Requirements of Fluid in Postoperative Nutritional Care Extensive fluid losses may occur through vomiting, haemorrhage, diesis, excudate, fever and sweating after a surg
Q. with no saturation of transport proteins and under the same concentration gradient how can the speed of simple diffusion is compared to the speed of facilitated diffusion? T
Requirements of Dental implants Dental implants require special oral hygiene techniques. The particular technique chosen often depends upon the prosthesis. A removable prosth
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