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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
what is the fate of corpus luteum if egg gets fertilised ?
Define Iron Status of the Individual Lastly, iron status of the individual is a floury determinant of how much iron is absorbed. On a mixed diet with some haem iron, the overal
Explain the Objectives of Taxonomy Let us summarise the objectives of taxonomy: 1. To inventory the world flora and fauna 2. To provide a method for identification and co
Question : (i) What is Ciguatera Fish Poisoning provide some examples of fishes implicated in Ciguatera Fish Poisoning in Mauritius? (ii) Provide a general account of its d
What are caloreceptors
Explain Metameric in detail. A body plan which consists of a series of identical units, metameres, repeated down longitudinal axis of the animal. Every metamere contains identi
Q. What are the cells produced in the first stage of the embryonic development called? The cells that result from the cleavage (the first stage of the embryonic development) ar
The metabolism of phenylalanine will now be taken in some detail, as two inborn errors of metabolism are known which affect this pathway. The Phenylalanine is 1st hydroxylated by p
In the metagenesis of Aurelia and Obelia what is the form that produces gametes? What is the form that reproduces asexually? In the metagenesis of some coelenterates, like Obel
Hydrochory - Dispersal of Seeds Plants that grow in or along the bank of water bodies often utilize water as an agency for dissemination of fruits and seeds. Coconut, Cocos nu
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