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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Can you explain Bayesian Theorem? Bayesian Theorem: The predictive value of a test is related to the incidence of disease in the population. A history of typical angin
What are the physiological changes during pregnancy
Animals of the open water zone The limnetic region of this zone contains certain fishes as well as rotifers, zooplankton such as crustacean and protozoan. In the profundal zo
excretory organs in spiders?
Explain Defensive Proteins These proteins protect against diseases. Antibodies are the example of defensive proteins. These combat bacteria and viruses. Also, immunoglobulins,
Explain the Process of dna technology Procedures involving recombinant DNA technology has been used to increase the yield of enzymes already produced by the microorganisms, e.
classifying kingdom of organisms
Q. In sickle cell anemia, a hereditary disease, there is replacement of one amino acid by another in one of the four polypeptide chains of hemoglobin. In this case are all of the s
Q. with no saturation of transport proteins and under the same concentration gradient how can the speed of simple diffusion is compared to the speed of facilitated diffusion? T
Q. What is genetic engineering? Genetic engineering, It is also known as molecular cloning or gene cloning, is artificial recombination of nucleic acid molecules in a test tube
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