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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
1. Carbon monoxide (CO): It is colourless, odourless, tasteless gas and is not soluble in water. Source: CO is produced due to: (i) Incomplete combustion of fuels
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Define the Composition of Potato Dextrose Agar? This media is used for cultivation of yeasts, fungi and moulds. Potato (Peeled) - 200.0 gm Dextrose - 20.0 gm Agar - 15
L A W S OF CLEAVAGE - 1. Sac's Law - According this law, during cleavage each blastomere to form similar blastomere. New cleavae always take place at the right angle of th
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Define endocytosis of GLUT4 Transporters Person X is a healthy human who has volunteered to take experimental drug Y. Person X has a normal dinner at 6 PM on April 1 and then
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Please show a diagram of the ATP molecule and label the major parts ot this molecule. Also, what kind of building block is ATP?
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