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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Distinct types of proteins Two distinct types of proteins are known: fibrous and globular proteins. Fibrous proteins are insoluble in water and are physically tough, which
Analysis of the written reports of service in relation to the fitness, quality and efficiency of the service should be provided. The routine record-checking is always insuffic
Define the Goals of treatment of Obesity? As said in the beginning, the goal of treatment of obesity today has shifted from mere 'weight loss' to 'weight management'. Each weig
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Similarities in body parts often indicate shared ancestry. Which of the following is true? Structures used for different purposes in different groups can be controlled by the same
Adrenal causes of hypertension are: 1) Excess of aldosterone production in primary aldosteronism. The diagnosis may be suspected when persistent hypokalemia is detected. Most of
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