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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are intraspecific and interspecific ecological interactions? Intraspecific ecological interactions are those among individuals of the same species. Interspecific ecologic
Q. Diagnosis of diabetes mellitus? Timely and proper diagnosis plays a key role not only in identifying new cases but also in managing old cases with or without diabetic compli
what term is used to indicate its grooves?
Bronchiectasis: Bronchiectasis literally means abnormal dilation of bronchi. It is a chronic inflammatory condition which is characterised by permanent dilation of bronchi a
Forestry : It concerned with protection or development of forest and to explore the outcome or economic potential of forest. Forestry is the art or science of tree resources, inclu
During the last 90 years, man-made materials and devices have been developed to replace parts of living systems in the human body. These special materials function in intimate cont
Quaternary Structure (4 o Structure). Globular in structure. When two or more than two molecules of protein of tertiary structure are connected to each other through
What are the differences and similarities between the bacterial and eukaryotic cell membranes?
Explain the term Direct Calorimetry? Calorimetry refers to the measurement of the amount of heat evolved or absorbed in a chemical reaction, change of state, or formation of a
Name the two groups of nephrons on the basis of their position in the kidney. How are they different from each other? a) How is the halophyte Rhizophora adapted to survive in i
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