Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Functions of the vascular system, Functions of the Vascular System The...

Functions of the Vascular System The major function of the circulatory system is to provide a transport mechanism for the blood flow to and from the heart. The vascular syste

Plasma Membrane, What can I do with this topic to make it into a brochure

What can I do with this topic to make it into a brochure

Metazoan branches, Metazoan Branches The Kingdom Animalia comprises al...

Metazoan Branches The Kingdom Animalia comprises all the multicellular animals (metazoans). These are divided into three branches: Mesozoa Parazoa Eumetazoa

Dna replication at biochemical level, Although there is much talk in the ne...

Although there is much talk in the news about stem cell research, the public and policymakers need to understand how basic body cells work to transmit information and replicate to

Describe the metabolism of lipids, Q. Describe the metabolism of lipids? ...

Q. Describe the metabolism of lipids? Fats are split into fatty acids and glycerol by the enzyme. They are absorbed in small intestine and recombined into fats. It is stored in

Do echinoderms present internal or external fecundation, Do echinoderms pre...

Do echinoderms present internal or external fecundation? Is there sex division among individuals? The fecundation in echinoderms is external; gametes are liberated in water whe

Dietary management of dyspepsia, Q. Dietary management of dyspepsia? Ke...

Q. Dietary management of dyspepsia? Keeping in mind the etiology, symptoms and complications of dyspepsia it must be clear that treatment and management of [his disorder does n

Foot as burrowing organ, Foot as Burrowing Organ The Scaphopoda that ...

Foot as Burrowing Organ The Scaphopoda that usually have burrowing habit, possess foot suitably modified for burrowing purpose. The conical and protrusible foot of Dentalium

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd