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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Cell - Cell is the structural & functional unit of life. Study of the structure of the cell is called cytology. Study of structure and functions of the cell is called
Q. Microorganisms on On basis of nutrient degradation capacity? On basis of nutrient degradation capacity: - Proteolytic: Microorganisms, which are capable of protein degra
Kinds of Succession - Ecology The ecological succession can be broadly classified into two kinds, on the basis of the nature of the habitat. Figure: Kinds of Success
What are autotrophic beings? What are heterotrophic beings? Autotrophic beings are those that can make their own food, i.e., that make organic material from inorganic compounds
Explain Fetal Development? The medical profession divides pregnancy into three-month stages called trimesters. At the end of the first trimester, the fetus is only about 5 cm l
Types of Variations - Somatogenic and Blastogenic Variation Changes in somatic parts of the body are somatic variations e.g. wrestler's muscle, circumcised penis of mus
The marine biomes consist of the earth's oceans and its associated areas like the shorelines, islands, reefs and estuaries. The marine waters contain about 3.5 percent salt, mostly
Describe the term - Ambulacral groove The groove that runs down the oral surface of each echinoderm arm and contain the tube feet. If the region contains a visible furrow, or gr
information on climbing beans
Devonian is the period of geologic time from 410 - 360 million years before the present. Life on land diversified, with amphibians appearing late in this period of time. Plants we
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