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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Chemical Stress Survival of cells is dependent on carrying out of a set of chemical reactions (metabolic reactions) in a particular order. This results in a net gain in mass a
What is pollen? Pollen grains are the male gametophytes of the phanerogamic (flowering) plants. Thus within the pollen grains the male gametes of these plants are produced by m
Suppose you treated butter with a fatty acid desaturase, an enzyme that removes hydrogen from fatty acids and creates double bonds. Please explain what would happen?
Messenger RNA (mRNA) are the proteins are not synthesized directly from the genomic DNA. Insteadof that an RNA template (a precursor mRNA) is constructed from the sequence of gene
At 1 AM, a researcher places a healthy squid giant axon in a bath of normal squid physiological extracellular saline and internally perfuses the axon with normal squid intracellula
Explain the Plating Method - Pure Culture Techniques? Plating method is one of the commonly employed methods for getting a pure culture. The procedure involves the separation
Define Etiology - Anorexia Nervosa? The exact cause of eating disorders is not known. It is multi factorial in origin in which the personality of the patient, family relationsh
Q. Dietary Fibre requirement during diabetes? Dietary fibre is that part of food which is not digested by the intestines. High fibre meals have shown to give the best glycemic
Question : (i) What is Ciguatera Fish Poisoning provide some examples of fishes implicated in Ciguatera Fish Poisoning in Mauritius? (ii) Provide a general account of its d
genral charecter and classificatin of porifera
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