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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Casein is the main protein in milk. There are three types of casein, one of which is beta casein. Beta casein comes in two main variants called A1 and A2. The order of amino acids
Define measuring body composition- Multiple isotope Dilution? Multiple isotope dilution (Total and extracellular body water): It is based on the principle that certain substanc
Define Nutritional Needs during Exercise? During Exercise: Addressing the nutritional needs during training is essential for optimal performance. The focus should be to:
Define the Operations of a Public Nutritionist? A public nutritionist can perform the following: In the hospital-based set up, she is a part of the team delivering thera
Define some Usual Doubts of Children related to Food? Before embarking yourself to go through this unit, here is an activity for you to perform, Talk to 2-3 school' boys and gi
Q. What is the structural depiction of a carboxyl group? Carboxyl groups have a carbon attached to one hydroxyl group by a simple bond and one oxygen by a double bond. The othe
Application : Off Pump Coronary Artery Bypass Surgery (OPCAB) CABG is done on epicai-dial vessels. Cardio pulmonary by pass is used only to get a still h
what is radula?
Birds and Mammals - Heart is 4 chambered. 2 auricles & 2 ventricles. Sinus venosus & truncus arteriosus absent. Arteriovenous heart. Double circulation i.e. pre
Reflexes It is used to assess the neurobehavioural status. Rooting reflex - Being a primitive reflex, if cheek is rubbed, the infant will turn his head into that dire
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