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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
the Mendel's first law? A Mendel's first law postulates that a characteristic (trait) of an individual is always determined by two factors, one inherited from the mother and th
Preparation for Hospitalization Prevention is a strong component of nursing care. Preparation prior to hospitalization is essential to make the transition from home to hospi
What are some diseases or genetic abnormalities caused by dominant genes? Why are severe dominant genetic diseases rarer than recessive ones? Instance of dominant genetic disea
what are the levels of divercity
Vibriosis/campylobacteriosis This is genital disease of cattle and sheep. The causal organism Campylobacter fetus is a gram negative, curved or spiral shaped bacteria having s
Determine the tenant of the Cell Theory Which of the following is not a tenant of the Cell Theory as postulated by Mathias Schleiden and Theodor Schwann? A. All organisms a
General Principles for Incisions The general principles for placing incisions so as to create surgical flaps remain the same regardless of the reason for creating them. These a
Define Procedure for Testing the Presence of Urea in Milk? 1. To 0.5 ml of milk add 0.5 ml of DMAB solution (prepared by dissolving 0.4 gm of p-dimethyl amino benzaldehyde po
MOLECULAR MECHANISM OF HORMONE ACTION - Once a hormone enters the bloodstream it can reach almost any cell in the body. However, each hormone affects only certain kinds of c
Treatment of susceptible tb All isolates of Mycobacterium tuberculosis should be tested for antimicrobial susceptibility, but results generally do not become available for at l
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