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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the Lactose Fermenters and Non lactose Fermenters 1) Lactose Fermenters - These produce acid on lactose fermentation, which results in red colouration on bacterial colo
General characteristics of phylum
Endocrine Glands
What is Enzyme assay Enzyme assay may also be used to determine whether stored plant products are suitable for use as food commodities, for e.g. α-amylase should be present
Explain the Use and Care of Microscope? Proper care and maintenance of microscope is needed. Following points should be kept in mind while handling the microscope: (i) Instr
REGULATION OF BLOOD GLUCOSE LEVEL Various levels of regulation are exerted at substrate level, hormonal level, enzymatic level and at organ level on carbohydrate metabolism s
A common biocontrol agent for the control of plant diseases is : 1. Baculovirus 2. Bacillus thuringiensis 3. Glomus 4. Trichoderma Trichoderma
PURPOSES OF ASSESSING GROWTH AND DEVELOPMENT IN CHILDREN Before you assess the growth and development you should be aware of the purpose of monitoring. The purposes are a
Q. Dietary Management during congestive cardiac failure? Nutritional care is a little difficult in congestive cardiac failure. This is because oedema complicates the nutritiona
How Campylobacteriosis is caused It is caused by Campylobacter jejuni, a Gram-negative rod. It is a microaerophillic organism, which means it has a requirement for reduced lev
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