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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Mobilization of bone calcium and phosphorous? It is now firmly established that vitamin D 3 is metabloized first in the liver to 25-hydroxyvitamin D (25 OH- D) (calcicl
General guidelines for use of BONE GRAFTING 1. The surgical site should be prepared, that is it should be free of granulation tissue. 2. For autogenous grafts- the donor sit
Ranking of Companies According to the Death Rate The death rates because of accidents vary from industry to industry. Following is the list of industries in order of decreasin
Q. What is the difference between the reptile heart and the amphibian heart? The reptiles have incomplete and double circulation three heart chambers two atria and one ventricl
Phylum-Bacteria (Eubacteria) Eubacteria,th e true bacteria, are extremely small and they exist in vast numbers virtually everywhere on earth. They range from the upper atmosphe
Gluconeogenesis Gluconeogenesis is the procedure through that glucose is synthesized from smaller easier molecules such as lactate and pyruvate. Although the catabolic linear p
what year did virchow state where a cell exists there must have been a pre-existing cell
What evidence is there that makes it seem humans have more than a material existence?
what is phylum protozoa
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