Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What is karyotype, What is karyotype? Name the karyotype is given to th...

What is karyotype? Name the karyotype is given to the set of chromosomes of an individual, generally when visualized and identified under the microscope. The visualization in g

How the couple is a carrier for a genetic disease, On a separate piece of p...

On a separate piece of paper, draw a simple family tree for a couple showing children (F1), and lineages from two children showing grand children (G2), great grandchildren (G3), gr

What is phosphorylation, Q. What is phosphorylation? What are various biolo...

Q. What is phosphorylation? What are various biological processes in which phosphorylation plays a critical role? Phosphorylation is the name given to procedure of the addition

Explain deficiency diseases due to vitamin c, Explain Deficiency diseases d...

Explain Deficiency diseases due to vitamin C? Vitamin C deficiency in human's results in the disease called scurvy, whose symptoms include hemorrhaging (especially in the gums)

Explain about the torbed dryer, Explain about the Torbed Dryer? On the ...

Explain about the Torbed Dryer? On the other hand, is a variation of the fluidized bed dryer. This is designed for use with particulate foods. Torbed dryers are used to dry haz

Criteria for implant success as given by albrektsson, Q. Show the Criteria ...

Q. Show the Criteria for Implant Success as given by Albrektsson? Criteria for Implant Success as given by Albrektsson are: 1. An individual unattached implant is immobile w

Choice of transgenic animal, C h oice of transgenic animal First and ...

C h oice of transgenic animal First and foremost is the selection of animal species in which gene of interest is to be transferred. Whatever is the goal of production of tran

Explain about ia muscle-spindle, Which of the following occur in response t...

Which of the following occur in response to an increase in the length of the right knee extensors in response to a quick tap applied to the right patellar tendon?  An increase in t

Point out some guidelines for breast-feeding, Point out some Guidelines for...

Point out some Guidelines for breast-feeding? 1) Follow demand feeding of the baby instead of a fixed schedule. 2) Initiate breast-feeding within 2 h after a normal delivery

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd