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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
A fifth member of the monoamine transmitter group is histamine. That release histamine as their neurotransmitter is located in the hypothalamus and project to cerebral cortex, lim
What is the best microscope to get a detailed view of the parts inside of a preserved plant cell
Are there chloroplasts in cyanobacteria? In cyanobacteria there are no chloroplasts and the chlorophyll layers are dispersed in cytosol. Which chemical element is central in
Define Precautions for Quantitative Determination of Viable Microbes? 1. Dilution should be made carefully. 2. Use fresh sterile pipette for making each dilution. 3. Asep
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Human Factor Theory - Accident Causation This theory of accident causation suggests that a chain of events caused by human error is the cause of accident. The chain of events
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why do we get severe leg pains when the internal bone is cracked?
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