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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Various ways/techniques suggested for control of water pollution are as follows: 1. Pollutants (radio active, chemical, biological) present in water bodies can be removed by app
Light and Distribution We have mentioned in the beginning that the variation in the amount of light generally affects the global and local distribution of plants and animals. L
NEO DARWINISM OR SYNTHETIC THEORY OF EVOLUTION - It is the synthesis of the ideas of Neo Darwinians as a new theory of evolution based upon recent findings of genetics and m
Explain some Effect of Inadequate nutrition? Inadequate nutrition ranks as one of the major problems of old age. Various factors which may be responsible for the change in one
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A fire hazards is any situation when there is a greater risk of harm to the people or the property due to fire. Causes 1. Overloaded electricity systems. 2. St
Q. How different is the simple cuboidal epithelium from the columnar epithelium? Where can these epithelia are found in the human body? The simple cuboidal epithelium is made o
Q. What are the conditions favouring the outbreak of Staphylococcal food poisoning? • Botulism is caused by the bacteria Clostridium botulinum. • All low acid foods can s
Internal budget - Nutrient Budgets This is concerned with the circulation of nutrients through various biotic and abiotic compartments of a given ecosystem. In other words the
Insect resistant crops (IRC) Insects are a natural selection pressure so plants resistant to certain insects could have an evolutionary advantage (in natural environments)
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