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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
VACUOLES They are of 4 types - 1. Sap Vacuole - Outer membrane of sap vacuole is called T onoplast . Sap vacuole contains amino sugars, carotenoids, proteins, m
What are Pure Culture Techniques? Microorganisms can be isolated from various natural environments like soil, food, water, sewage, decomposing matter and even from infectious m
Class Gastropoda Body asymmetrical, depicts torsion or its effects; shell coiled in most, well developed head with radula, large flat foot, gills one or two or with pulmonary
Q. What is self-catalytic RNAs? Ribozymes - Term 'ribozyme' was initially suggested by Thomas R. Cech, Nobel Prize winning biochemist, who discovered this class of RNA molecul
Regarding the article "One Hundred Tests" which of the following is a false statement? A. Genetic tests like the one developed by Counsyl can examine the multilevel genetic net
Procedure of radiographic template It is very similar to a flasking procedure. Mix slow setting irreversible hydrocolloid (alginate) with cold water (to gain more working ti
Q. How different is the simple squamous epithelium from the stratified squamous epithelium? Where can these epithelia are found in the human body? The simple squamous epitheliu
LIGHT All of us know that the sun is the ultimate source of energy for all activities in our biosphere. The electromagnetic radiations from the sun supply energy which warms up t
natural selection
Q. What are the major biological functions in which chlorine ions participate? Like sodium cations, chlorine anions actively participate in the regulation of the osmolarity of
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