Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What is the evolutionary importance of pteridophytes, What is the evolution...

What is the evolutionary importance of pteridophytes? As the first tracheophytes, pteridophytes were also the first plants to extensively colonize the terrestrial environment f

Express rate of mutation of a given species, Select all of the correct/true...

Select all of the correct/true answers. The rate of mutation of a given species can be predicted. Mutations are a source of new alleles and contribute to the biodiversity present o

Snake bite, S n ake bite Biting by a poisonous snake is manifested by...

S n ake bite Biting by a poisonous snake is manifested by clinical findings of local swelling and nervous symptoms. E t iology: The poisonous snakes have different typ

Experiment the test for limestone, The test for limestone You can chec...

The test for limestone You can check the rock samples to see if any are limestone by dropping lemon juice, vinegar or some other dilute acid on them. If any are limestone they

Flaws in developmen of human, Flaws in Developmen of Human The human ...

Flaws in Developmen of Human The human growth and development in spite of its complexity works perfectly most of the time. But while development goes awry it creates a giant

What is mitral stenosis, Q. What is mitral stenosis? Most common cause ...

Q. What is mitral stenosis? Most common cause of mitral stenosis is rheumatic fever. Nearly 30 per cent of patients with rheumatic fever may go on to develop pure mitral valve

Aril - seed appendages, Aril - Seed Appendages It is an outgrowth that...

Aril - Seed Appendages It is an outgrowth that arises from the funicle or the testa near the raphe and covers the seed partially or completely. It is often referred to as the

All or None Response, All or None Response A very weak stimulus does n...

All or None Response A very weak stimulus does not result in an action potential. The stimulus must have a certain strength to evoke an excitation. This is the threshold value

How different are fecundation in chondrichthyes, Q. How different are fecun...

Q. How different are fecundation in chondrichthyes and in osteichthyes? In chondrichthyes fecundation is internal by resources of copulation. In osteichthyes fecundation genera

Describe the properties of gum ghatti, Describe the properties of gum ghatt...

Describe the properties of gum ghatti Important property of gums referred to as 'viscosity' and the effets of pH. Gum ghatti does not form true aqueous solutions but form visco

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd