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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is Inoculation and Culturing? In microbiology, inoculation is the process where a microbe of interest is introduced into a previously sterilized growth medium for the p
what is cockroach respiratory system?
In what forms are nitrogenous wastes excreted in birds, humans and aquatic turtles respectively ? Why so, describe?
Define Nutritional Status of the Elderly? Nutritional risk factor is defined as "characteristic or occurrence that increases the likelihood that an individual has or will have
abnormal lung volumes and capacities
What are stains? Microorganisms are difficult to be seen in living state because of their minute, colourless and transparent nature. Moreover, there is limitation of insufficie
Define Birth weight - etiological factors for obesity? It has been shown that slow growth of the foetus in utero and during infancy is followed by accelerated weight gain in ch
why is it known as middle man
Determine fat-soluble vitamins Recommended for Infant? ICMR (1990) does not give recommendations for vitamins D, E and K. As you already know, vitamin D is expected to be met
A kinase is in general an enzyme which catalyzes the transfer of the phosphate group from ATP to something else. In the molecular biology, it has acquired the more specific verbal
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