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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the Burden of Hypertension in heart diseases ? Since 1942, there have been several small and large population-based studies on Hypertension. A ineta-analysis showed an
Are bacteria the only prokaryotic beings? Prokaryotic beings are classified into two big groups: archaebacteria and bacteria (this last also known as eubacteria). Compared
Explain what is Genetic Factors ? It has been suggested that certain genetic defect(s) could be partly responsible for increased risk of CAD in Asian Indians. No specific genet
Polarity in Regeneration A distinct polarity or gradient exists in planarians (and in other animals). The meaning of this is that in any piece or segment of the body; its ant
Q. Are there any bacteria made of more than one cell? There are no pluricellular bacteria. All bacteria are unicellular prokaryotic.
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Indications for Surgery : 95 per cent of ventricular aneurysms occur after transmural myocardial infarction. Trauma, Chaga's disease, sarcoidosis and congenital causes are the ot
Integral plasma membrane proteins are also synthesized by ribosomes on the RER, but become inserted in the rough endoplasmic reticulum membrane rather than transported into the lum
Question 1 Describe in detail the immunoprophylaxis Question 2 Give a detailed account on autoimmune diseases Question 3 Describe the classification and properties of an
Differential reinforcement of other behaviour (DRO) This is used to decrease frequent behaviour by reinforcing any behaviour other than the undesired one. An instance would be r
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