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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Aiscommoncellismwhatsk question #Minimum 100 words accepted#
Table provides you the classification of the living primates. The primate fossil history dates back to 60 million years i.e. to Paleocene times, more specifically to the cretaceous
Define briefly about the Nuts as protein source? Nuts are excellent sources of proteins. Examples of nuts include cashew nuts, almond nuts , hazel nuts , coconuts, walnuts, bra
Explain about the Cheese Making - methods of food processing? Cheese is a way of preserving milk for long periods of time. In this process, the milk in cheese becomes something
All Solid Semi-Solid Wastes Arising From Human And Animals Activities, Except Human Excreta And Sullage (Liquid Wastes From Bathrooms, Kitchens Etc.) are discarded as useless or un
Q. Complications during angina pectoris? It is a symptom giving a warning of impending myocardial infarction, sudden cardiac death or even ischemic necrosis of the brain leadin
Why is the cerebellum more developed in mammals that jump or fly? The cerebellum is the major brain structure that coordinates the movement and the equilibrium of the body. For
Supra Vulvar Aortic Stenosis : Supra valvar aortic stenosis in children with elfin facies, mental retardation, multiple peripheral pulmonary artery stenosis and hyper calcem
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What is karyotype? Name the karyotype is given to the set of chromosomes of an individual, generally when visualized and identified under the microscope. The visualization in g
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