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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Does an increase of H+ cause the hemaglobin oxygen binding curve to shift to the left or right? Explain.
Describe Prostaglandin - Availability, Administration and Alternatives ? Prostaglandin El (available in India as Prostin VR, Pharmacia) is a very essential drug and should be a
Q. What is wall resistance of plant cells? Does this resistance make difficult the entrance of water into the cell or facilitate? Turgor Pressure TP, or Wall resistance, is the
What is an analogy for a smooth endoplasmic reticulum? Endoplasmic reticulum (ER) is such as a manufacturing plant, like a factory, where proteins and lipids are made. This is
The cell cycle undergoes a sequence of changes which involve a period of growth replication of DNA, Followed by cell division. This sequence of changes is called cell cycle.
why phloem made of living cells
Explain Iron Balance and Regulation of Iron Absorption? The body has three unique mechanisms for maintaining iron balance. The first is the continuous reutilization of iron fro
Define Role of Food Industry? Publications of major trade associations; industry standards; structure of the food industry; international food corporations; information on alli
Question 1 Write a short note on the following- Totipotency Soma clonal variation Bioreactor Virus indexing Question 2 List and explain any 4 advantages of
List the types of Gellan gum. The three types of gellan gums are: High acetyl gellan (partially deacetylated) Low acetyl gellan (highly deacetylated) High cl
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