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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Symptoms: The E. coli gastroenteritis syndrome is caused by the ingestion of 10 6 -10 10 viable cells/g that must colonize the small intestine and produce enterotoxin. The syndr
Locomotion in Arthropoda The Arthropods are characterized by the existence of some special features which can be considered key to their success. These involve a rigid exoske
I need help with making a taxonomic key
Explain the Colorimetric Method Glucose Estimation? Alkaline copper reduction methods are by far the most commonly used methods for glucose estimation. This time honoured metho
VITAMIN - K Also known as naptho quionone / anti haemorrhagic vitamin / coagulation vitamin / menadone . It requires for formation of blood clotting factor prothrombin.
short notes on cholesterol.
Explain what is DNA? DNA : DNA is the source of the information that directs the development of the cell. Although all segments of DNA are not active in every cell in the huma
General Characters-
Define ascending type of paper chromatography? The ascending type consists in dipping the lower end of the paper containing the sample spots into the solvent so that it is abov
cellular respiration
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