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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
In the sarcomere of a skeletal muscle, there are A. myosin molecules in the I band. B. both tropomyosin and myosin molecules in the region of the A band that is not in the H
Can you help explain this question in fairly simple terminology? A certain sample of DNA has a Tm of 65oC, while another has a Tm of 58oC. What can you tell about the relative n
Q. Nutritional Management in Inflammatory Bowel Disease? Adequacy of nutritional needs and minimizing stress on the inflamed or narrowed segment of the bowel are the main princ
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Which of the following patients will likely experience difficulty in maintaining lipoprotein synthesis resulting in elevated LDL levels? A physician is explaining to a group of med
Q. How CAD Patients Response in Hypertenstion? An exercise rise in systolic blood pressure (over 200 mm Hg) has been used as a reason to terminate exercise in some centers. She
Pathology: This concerned with the identification of different diseases, causative organs, their prevention and control methods. Pathology is a kind of study or diagnosis of diseas
Question 1: Write the applications of Oracle clinical in Pharmaceutical industries Explain what is "Oracle clinical" Explain different application of oracle clinical
What are the four types of weak bonds, and how do they differ from each other and from covalent bonds?
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