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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define effect on Oestrogens and progesterone in pregnancy? Oestrogens and progesterone are secreted by the placenta from 8-10 weeks of gestation. Progesterone increases through
Q. Rate Related Bundle Branch Block? The term has often implied the absence of significant coronary or myocardial pathology. It cannot be judged without taking the total clinic
Q. How does the female gamete move from the ovary to the uterus? The female gamete released from the ovary falls into the surrounding abdominal cavity and is collected by the F
Define Male and Female Ascaris Lumbricoides? Ascaris lumbricoides unlike most free-living nematodes and a large number of parasites of plants and animals are not tiny or micro
If water dousing, homeopathic cures, and so on work for just me but not for anyone else, it is still science.
Determine number of sucrose molecules in the solution When a solid is dissolved in a volatile solvent the vapour pressure of the solution is less than the vapour pressure of th
Q. Describe Coping Skill and Coping Mechanism? Coping Skill is a tool which may be used by individuals in any difficult situation like solving a problem by choosing best soluti
Explain the different types of cells in cell structure ? Types of Cells : Cells can be classified as being either prokaryotic or eukaryotic depending on whether or not a mem
MUSCLES - In eye orbit, eye ball is fixed by 6 skeletal muscles attached to 3rd, 4th & 6th cranial nerves (motor). 4 - straight or recti muscles are present. 2 - oblique
What is the Importance of biotin Biotin is involved in a number of important metabolic reactions, probably as coenzymes. Deficiency symptoms are manifest as degenerative cha
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