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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Illustrate Stents? Stents are metallic scaffolds that are deployed within a diseased segment of a coronary artery to establish and then maintain a widely patent lumen. Stent
Explain Diffusion and Osmosis in cell structure? Diffusion and Osmosis : Diffusion through a cell membrane occurs as it does elsewhere, from an area of high concentration of
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Copper deficiency Copper deficiency may be primary or secondary (conditional). Primary copper deficiency occurs due to its inadequate dietary intake; whereas secondary copper d
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Q What is the usefulness of fungi for some industries? Fungi are industrially used in the production of fermented beverage, cheese, bread, and so on. A few fungi are very impor
Conduits Figure: Venous Conduits Reversed saphenous vein was the first conduit used for CABG. 'It is usually harvested from the leg starting above the ankle going up
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