Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What are the penetrance and the expressivity of a gene, What are the penetr...

What are the penetrance and the expressivity of a gene? The Individuals that carry a same genotype don't always manifest in an identical manner the correspondent phenotype. The

Report writing, tell me how to write a report in molecular biology?

tell me how to write a report in molecular biology?

Causes of degradation of ecosystem due to agriculture, Causes of Degradatio...

Causes of Degradation of Ecosystem Due to Agriculture Degradation of the ecosystem due to agriculture can be on the following accounts: a) Removal of trees (deforestation

Define the intradermal dye test, Define the Intradermal Dye Test? The r...

Define the Intradermal Dye Test? The rate of decolouration of a small quantity of the dye, 2, 6- dichlorophenol injected intradermaly is an index of the ascorbic acid nutrition

Nutrition in animals, composition,sources,importance,types,requirements for...

composition,sources,importance,types,requirements for each food substances

What is global warming, What is global warming? Global warming is the e...

What is global warming? Global warming is the enhance in the temperature of the planet due to accumulation of some gases in the atmosphere, especially gases that retain the sol

Determine the eukaryotic replication origins, Which of the following statem...

Which of the following statements is wrong regarding eukaryotic replication origins? A. More origins are licensed and initiated within embryonic cells than in adult cells. B

How to increase in sodium conductance, How to increase in sodium conductanc...

How to increase in sodium conductance A complete motor neuron is removed from a frog and placed in normal physiological saline at 1 AM.  The neuron is healthy.  At 2 AM, the ph

The movements of the foetus in which month of pregnancy, The first movement...

The first movements of the foetus and appearance of hair on its head are usually observed during which month of pregnancy? 1. Fourth month 2. Fifth month 3. Sixth month

Under which forms is the trypanosoma cruzi found, Under which forms is the ...

Under which forms is the Trypanosoma cruzi found in its hosts? In the definitive hosts as well as in triatomine bugs (middle hosts) the protozoan that causes Chagas' disease ch

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd