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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define the Role of Vitamin D in the immune system? Immune responses that are, mediated by T-cells can be inhibited by tile large doses of calcitriol i.e. 1, 25 dihydroxycholec
What are sols? A colloidal system in which solid particles are dispersed in a liquid is referred to as a sol, to distinguish it from a true solution. In a true solution, the
Q. Where are the chemoreceptors that detect the acidity of the trigger and blood the respiratory compensation located? The chemoreceptors that participate in the ventilation co
Alkaline Phosphatases use water as a second substrate. Why do we still treat their kinetics as if they were for a single-substrate reaction?
What is the concept of Lophophore? A unique double ring of hollow ciliated tentacles that surround the oral opening in some animal phyla. There is some debate as to whether the
List the five major phases of the cell cycle, and briefly explain what happens during each phase. G1 phase: the cell grows. S phase: DNA is copied. G2 phase: the cell prepares
How is retaining wall works? A retaining wall together with the backfill the wall retains and the soil that supports the wall is a highly indeterminate system. The magnitudes o
You wish to use PCR to sequence a particular gene that you have isolated. The sequencing protocol suggests that you use 3.2pmole (pico moles) of your primer in a 2 micro liter volu
Neuron A is a healthy neuron with all the usual ion channels. When at rest with a membrane voltage of R millivolts, neuron A produces no action potentials. The voltage thres
What are the main constituents of the earth's atmosphere in our time? The present atmosphere of the earth is constituted mostly of molecular nitrogen (N2) and molecular oxygen
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