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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Implant Integrity Implant Integrity: A horizontal dark line at the abutment level is most probably due to screw-loosening and separation of elements. This may happen
Define Homogenization or Blending of Food Sample? Food homogenization - Liquid food samples are mixed manually before analyzing whereas solid foods are homogenized in a diluent
are there non-parasitic viruses
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Apex locator in retreatment cases: - Electronic apex locators may give misread for working length when gutta-percha is initially being removed. - Due to that file beging
Q Which are the organs and characteristics of the digestive system of earthworms related to the kind of diet of these animals? Small organisms ingested together with soil parti
Anthrax Synonym : Wool sorter’s disease Species affected : Cattle, sheep, goats, pig, horse and majority of the mammals. Fowls are resistant to anthr
Classify phylum mollusca?
What is the difference between the concepts of karyotype and genome? Genome is the set of DNA molecules that characterizes each living being or every species. The concept then
Name the reserve food materials in pheophyceae Ans) Reserve food materials in pheophyceae are laminarin and mannitol
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