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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Congenital Anomalies of Small and Large Intestines: 1) Intussusecption Intussusecption is the invagination or telescoping of one part of the intestine into another part
Biotic Balance of Nature A nineteenth century Biologist is reported to have expressed, Glory of Britain is due to its old maids He reasoned Healthy Britons are nourished by roa
Relate the oxide layer and biocompatibility of titanium. The compatibility of a metal with its host environment depends on its resistance to biodegradation and on the degree of
Q. Prevention of Coronary Heart Diseases? In view of the steep rise in the incidence of chronic degenerative coronary heart diseases, several programmes have been formulated to
Indications for Surgery : Congenital pulmonic stenosis is the most common lesion requiring relief. Very rarely it could be due to rheumatic involvement along with disease of othe
Initiation Codon is the codon at which the translation of a polypeptide chain is initiated. This is generally the first AUG triplet in the mRNA molecule from 5' end, where the rib
What are plankton, nekton and benthos? Plankton, nekton and benthos are the three groups into which aquatic living beings may be separated. The plankton is formed by the alg
Characteristics of Nutrient Uptake These results show certain characteristics of nutrient uptake. Selectivity: Certain mineral elements are taken up preferentiall
GAS T RUL A TIO N - Morphogenic movements are occured. 3 germinal layers are formed. Ectoderm, endoderm & mesoderm. Endoderm is formed first. Mesoderm is formed in the
If a solution has a pH of 7.5, what would its new pH be if the concentration of H 3 O ions in the solution were increased by 100 times? Explain your reasoning. As a tenfold inc
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