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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How many pairs of electrons do the two oxygen atoms in an oxygen molecule share with each other? Explain your answer. The oxygen atoms share two pairs of electrons, sinc
How Vitamin E is directly related to selenium and PUFA? Selenium: An inter-relationship exists between vitamin E and selenium, as selenium functions as an integral par1
Which actions proposed in 1994 were not finalized in the recently issued plant- incorporated protectants rules ? EPA has shown in a supplemental notice, which is part of the fi
Genes and Alleles The inheritance of any character can be studied only when thcre are two contrasting conditions, such as yellow versus green seed colour (as observed by Mendel
Leishmanias – Flagellates Species of the genus Leishmania are parasites of mammals including man. Leishmanias infections occur over wide regions of the world from Asia, the ne
what is the main quality of this phylum
Q. Can you explain Tannins? Tannins are another class of compounds which interfere with the absorption of minerals like iron and reduce the availability of proteins by bindin
What is the lasting form in the gametophyte, pteridophytes or the sporophyte? How can it be compared to bryophytes? The lasting form in pteridophytes is the diploid (2n) sporop
Explain about lyophilic If the affinity between the dispersed phase and the medium is high, the dispersed phase is said to be lyophilic (solvent loving) or hydrophilic, in the
Explain about the cyclamate - artificial sweeteners? Another sweetener is cyclamate (sodium cyclohexylsulphamate). Have a look at its structure as shown in the figure. It is on
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