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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How Thirst and satiety influence water intake? Thirst and satiety influence water intake, apparently in response to changes sensed by the mouth, hypothalamus and nerves. When t
Define the Term Epimerism? In addition to D and L isomerism sugars also exhibit epimerism. Hexoses that differ from each other as a result of variation of H and OH on carbons 2
Technological level - Pollution control Air pollution can be reduced by using bag filters, electrostatic precipitators and cyclone separators that separate large and small par
D o w n e r s cow syndrome The Downer's cow, otherwise known as creepers, is commonly a fatal condition of dairy animal associated with the unsuccessful treatment of cas
What is anemia? What are the four main types of anemia? Anemia is low concentration of hemoglobin in the blood. The four main types of anemia are the nutrient-deficiency ane
Independent assortment has which of the following effects on the inheritance of alleles? a.Alleles on the same chromosome are not always inherited together. b.Alleles on diff
Discuss the biological importance of Imidazole. Bring in examples of biomolecules that contain this group. Please be as thorough as possible.
Carboxypeptidase The inactive zymogen procarboxypeptidase is activated by trypsin .The further action on the polypeptides is carried out by carboxypeptidase which attac
Estimate the Results of Tetralogy of Fallot? In excellent centres, the mortality is reported one per cent or less. In infants and babies with low weight, higher mortality is ex
What are the differences between vertebrates and the other chordates? Vertebrates are dissimilar because they have a spinal column (vertebral column). In these animals the noto
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