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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
M A TERNIT Y AND CHILD HEALTH & FAMILY PLANNING - 1. The health centers give necessary information, guidance & assistance to mother before & after delivery pregnant wo
Explain fats requirements during thyphoid Fats: Use of fats should be in moderation. This is because the typhoid patient has a compromised ability to digest and assimilate due
N a ture of viruses Viruses vary in their size ranging from 20 nm (200 Ao) to 350 nm (3,500 Ao) and in shapes from spherical to bullet, rod, brick or filamentous forms. Some
Ovipary, Vivipary and Ovovivipary In each case of external and in some cases of internal fertilisation, development of the fertilized egg (zygote) occurs outside the mother's
What are the plant root hairs? Where can they be found and what is their function? The root hairs are external elongated projections of the root epidermis. Their role is to
Explain Adverse Effects of foscarnet Renal dysfunction often develops during treatment with foscarnet and is usually reversible, but renal failure requiring dialy sis may occu
How the blood is circulated with the help of Heart?
DIFFERENCE BETWEEN LIVING AND NON-LIVING BEINGS - S .No. C h a ra cte r L i v i n g be ings N o n - l i v i n g be
While waiting at an airport , nail Campbell once overheard this claim, its paranoid and ignorant to worry about industry or agriculture contaminating the environment with chemical
Tonicity and the plant cell. Table 3: Amount of water displaced by potato samples before and after experiment?
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