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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
(a) Which elements contains Carbohydrates (b) Which elements contains Proteins a) Carbohydrates contain the elements carbon , hydrogen and oxygen. b) Protei
SIGNIFICANC E OF GASTRULATION - It results in the formation of three germ layers. It also resutls in the formation of different organs & organ systems. Gastrulation is e
Eric Davidson isolated and compared the sequences of mRNA transcripts and genomic DNA from the sea urchin. Which of the following is a true statement regarding his findings? A.
Production of Disease-free Plants Under normal conditions plants are infected by a wide range of pathogens such as bacteria, fungi, viruses, viroids, and insects like nematode
What is the formula of the DPD for withered (shrank) plant cells? How is that situation possible? Ans) Withered plant cells are those that shrank because of loss of water by eva
Totipotency and Pluripotency In the starting we said that the fertilized egg cell (zygote) has the capacity or potentiality to give rise to all kinds of cell types, like a bl
Which of the following processes help bring oxygen to the body cells that are in a leg? A. Net flux of oxygen from red blood cells into blood plasma in the body capillaries in
What are the chemical elements that form most of living biological matter? The elements are:- a) oxygen (O), b) carbon (C), c) hydrogen (H) and d) Nitrogen (N).
Q. What is parthenogenesis? Parthenogenesis is the formation or reproduction of a new individual from the egg cell but without fecundation by the male gamete. According to the
EGG S OF HEN It is a meiolecithal egg which is oval. The animal pole is found in the form of a small germinal disc. The vegital pole has two types of yolk viz. White yol
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