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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Enumerate the Sizes and shapes of eukaryotic and prokaryotic cells It is thus possible, due to these similarities, to generalise about the structures and functions of cells. In
Armamentarium 1. Denture duplicator or a modified plastic soapdish. (A plastic large soapdish with a base and cover can be adapted by drilling 2 mm holes interspersed and cover
What is the central dogma of molecular biology? Succinctly (and clearly) describe the concept.
Syngamy The pollen tube grows to a very limited extent in the synergid. It releases the contents either through a terminal or a subterminal pore. The contents include the two
Width of the Implant The width of the implant especially at the interface area is critical towards the success of the implant. It has been recommended that at least 1mm of bone
Determine the Effect of Iodine Deficiency? Iodine deficiency affects all populations at all stages of life, from the intrauterine stage to old age. However, pregnant women, lac
What are the organic chemical groups that characterize carbohydrates? How are carbohydrates classified according to the presence of those groups? Carbohydrates are also called
Collection of Cerebro-spinal Fluid (CSF) Examination of the CSF is one of the most useful laboratory procedures in neurological diagnosis. Four types of punctures ma
Limb Regeneration in Amphibians In vertebrates, the amphibians particularly the urodeles comprise spectacular power of regeneration. This power of regeneration has made them
Driving Force - Mineral Nutrition Let us now find out what is the driving force involved in protein mediated transport. Many membrane transport proteins allow specific solute
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