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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Preparation of Dilutions - Standard or Total Plate Counts? 1. Serial dilution of homogenized sample in an appropriate diluent. Preparation of dilutions - Decimal dilu
what is the classification of skeletal tissue?
Traumatology : This is the study of wounds. In other words we can say that traumatology is the study of wounds or injuries which can be caused by accidents or violence to a person
Programmes directed towards the common crop plants These programmes can be directed towards incorporating the following traits among common crop plants. Non-photo
What is the meaning of Food Exchanges Food exchanges mean grouping of foods in which the specified amount of all the food provides approximately equal amounts of carbohydrate,
give main charecters of protozoa
Q. What do you mean by hydraulic modelling? Modelling is neither new nor relevant to hydraulic engineering only. It is practiced in the field of hydraulic machinery for testing
what is endosperm haustorium
Aortic Valve replacement : The initial steps of the operation have been described earlier. Ascending aorta is cannulated. A single two-stage atrio-venous cannula is inse
Determine Procedure for determination of protein content? Now carry out the procedure as enumerated herewith: 1. Preparation of standard albumin: A standard solution of 2 mg
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