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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the uses of hydrocolloids In particular, hydrocolloids can provide water for increasing the flexibility (plasticizing) of other food components. These can also affect i
Echocardiography Utilizes principle of ultrasound for visualization of heart and great vessels. It uses sound in the frequency of 1-10 MHz. As the frequency of probe increase
Formation of pronuclei : Information of pronuclei, micronucles which is one of the conjugants, plays an important role. Micronucleus undergoes meiosis and forms four haplo
In general compare and contrast the three functional classifications of joints according to movement. What are two characteristics that make synovial joints unique and different fr
PRINCIPLES OF CARDIOPULMONARY BYPASS MECHANISM (CPB) What is CPB? It is a technique by which the mechanical function of the heart and respiratory function of the lungs
How can a baby become infected with (a) gonorrhoea, (b) syphilis (a) During birth, a baby might be become infected with gonorrhoea bacteria as it passes by t
CHARACTERICTICS OF PLATYHELMENTUS
Explain about the Anorexia Nervosa? Anorexia nervosa, as you have read above, is a psychological eating disorder characterized by somatic delusions that one is too fat despite
What is ascaris? What is the disease caused by this worm? Ascaris, or Ascaris lumbricoides, is an animal of the nematode phylum, i.e., a roundworm. Ascaris causes ascariasis, a
what is bowman''s capsule?
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