Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Definition of a neuropsychological test, Definition of a neuropsychological...

Definition of a neuropsychological test The best definition of a neuropsychological test has been offered by Ralph Reitan, who described it as a test that is sensitive to the

Transferring the patient form icu to ward, Transferring the Patient form IC...

Transferring the Patient form ICU to Ward The patient is made to ambulate in ICU before shifting to ward . vitals are stabilized. The ward nurse is explained the pa

Explain the determination of hydrolysis, Explain the Determination of Hydro...

Explain the Determination of Hydrolysis Hydrolysis of calcium pantothenate in acidic medium produces  α,γ-dihydroxy-β,β-dimethylbutyrolactone, which reacts with hydroxylamine i

Explain deteriorative changes in fats and oils, Explain Deteriorative chang...

Explain Deteriorative changes in fats and oils The food products undergo changes in flavour due to the chemical changes occurring in fats and oils present in them. The causativ

Phylum arthropoda, PHYLUM ARTHROPODA Definition and Introduction ...

PHYLUM ARTHROPODA Definition and Introduction Bilateral  and protostomial eucoelomate  eumetazoa with metamerically  segmented  and each  segment bearing a pair  of  join

Why the pancreas is considered a mixed gland, Q. What is a mixed gland? Why...

Q. What is a mixed gland? Why the pancreas is considered a mixed gland? Mixed gland is a gland that produces exocrine and endocrine secretions. The pancreas is an example of

Precautions for estimation of sucrose by fehling soxhlet, Explain Precautio...

Explain Precautions for Estimation of Sucrose by Fehling Soxhlet Method? 1. Maintain proper temperature of the water bath for complete hydrolysis. 2. Add distilled water to

Explain the digestive system, Explain the Digestive System ? The diges...

Explain the Digestive System ? The digestive system takes in food and processes it. Digestion is the mechanical and chemical breaking down of food into a form that can be used

Define type of root canal perforations according to location, Define Types ...

Define Types of Root Canal Perforations According to location According to location (level) in relation to crestal bone): Coronal perforation: (supragingival) coronal to

Oceanic islands, Oceanic islands are interesting and somewhat specialised b...

Oceanic islands are interesting and somewhat specialised biomes. Islands which have broken away from the main continents have similarity of flora and fauna related to the continent

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd