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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the major classes of lipids The major classes of lipids are simple lipids (acylglycerols and waxes); Compound lipids (glycerophospholipids); Derived lipids (compounds t
history of biology
WHAT ARE THE DIFFERENT CULTURE MEDIAS USED FOR ISOLATION OF ACTINOMYCETES?
General characteristics of phylum
Explain Clinical dietitians Clinical dietitians, sometimes called therapeutic dietitians, are associated with health care institutes, hospitals and nursing homes. Depending on
Atmosphere is a multilayer blanket of various gases such as N2 (78.80%), O2 (20.95%), Ar(0.93%), CO2 (0.03%), water vapor and some other gases. This envelope extends from earth's c
ARTIFICIAL INSEMINATION - Artificial insemination is a technique to make a female pregnant by artificially introducing semen into the vagina. Artificial insemination has
How is the depolarization of the neuronal plasma membrane generated? How does the cell return to its original rest? When the neuron receives a stimulus by the binding of neurot
Define two major aspects of nutrition economics? There are two major aspects of nutrition economics. These aspects are: 1) Quantities of food commodities and their developme
What has caused evolution? 1) Different individuals have dissimilar characteristics 2) These differences result in altering abilities to reproduce under local circumstances
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