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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
The destruction of the ozone layer may be responsible for an increase in
What type of bonds is susceptible to hydrolysis? Book examples are C-N and C-OH.
Q. How many ATP molecules are made after glycolysis? Glycolysis is a process similar to glucose degradation in fermentation it produces final balances two molecules of ATP for
DEVELOPMENTAL DISORDERS - 1 . AMNIONITIS- Inflammation of amnion due to infection. 2 . ABORTION- Escape of a embryo prior to the stage of viability (ab
Define the Concept of Nutrition? Nutrition may be defined as the science of food and its relationship to health. It is concerned primarily with the past played by nutrients in
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Define the Incubator - Food Microbiology? The growth of the microorganisms is altered by the chemical and physical nature of its surrounding. Most important environmental featu
What is the evolutionary importance of pteridophytes? As the first tracheophytes, pteridophytes were also the first plants to extensively colonize the terrestrial environment f
What are epithelial tissues? What are their general function and how is that function associated to the features of the tissue? Epithelial tissues, also known as epithelia, are
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