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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain about the Absorbed Moisture - water found in food? It is the moisture that has passed through cell walls and entered the cytoplasm of the cell. It is this form of water
notochotr is absent in the group ...........
What is the evolutionary importance of the emergence of seeds in the plant kingdom? The evolutionary significance of the seed is related to the plant capability of distant colo
Indications for Surgery : The normal mitral valve area is 4-5 cm 2 . Usually symptoms appear when the valve area has become less than 2.5 cm2. Symptoms are present at rest when t
AGA R - AGAR It is cell wall mucopolysaccharide of some red algae (e.g. Gelidium, Gelidiella, Gracillaria). Agar is used as culture medium, thickener, stabilizer and as lax
How Viruses Multiply? Obligatory parasitism - Outside cells viruses are nonliving, inactive particles but after entering into live cells these multiply fast by replication
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What are the values of DPD for plant cells under hypertonic, isotonic and hypotonic media? In plant cells under hypertonic medium there is loss of water for the exterior, SF >
Differences between Prokaryotes and Eukaryotes It is obvious that there are a number of differences between them. Prokaryotes, like bacterial cells are generally much smaller,
Mineral availability of organic mineral complexes A number of mineral chelates and complexes are available from a variety of manufacturers. A chelate is described as a metal c
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