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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
A snapdragon plant that bred true for white petals was crossed to a plant that bred for purple petals, and all the F1 had white petals. The F1 was selfed. Among the F2, three other
In a test cross: A.alleles of two genes is always involved. B.One parent must be homozygous recessive C.the physical manifestation of a trait can be deterimined. D.both A and C are
Determine about the term - Copper Copper is known to act as an electron carrier in enzymes which bring about oxidation - reduction and regulates respiratory activity in plant
Open Heart Surgery These surgeries are done under alternative arrangements to continue oxygenated systemic blood supply, while the heart is operated on (Cardiopulmonary bypas
Q. Evaluating Inducible Ischaemia after revascularisation? Most early treadmill stress tests are performed either at discharge or within two weeks of an MI ard terminated with
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Define the Classification of Proteins? Proteins vary widely in. their properties. We suggest you look up this unit once again now as the information about proteins and their pr
What is the importance of the uterine glycogen-producing glands? The uterine glands produce glycogen that can be degraded into glucose to nourish the embryo before the complete
Materials Handling and Storage Material handling is a very significant process in working place. The materials are handled in not finished, semi finished and finished states.
Explain about the Fermentation - methods of food processing? In contrast to other preservation methods, multiplication of microorganisms and their metabolic activities are enco
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