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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are the three main types of nitrogen wastes excreted by living beings? The major nitrogen wastes excreted by living beings are ammonia, uric acid and urea. Living beings t
Relationship of Ecology with Other Disciplines of Biology In order to understand the scope and relevance of ecology let us consider its position in relation to other biological
What are cytokinins? Where are they made? Cytokinins are phytohormones active in the promotion of cellular division; they slow down the aging of tissues and act together with a
2.?In order to increase muscular force production, muscular contractions may be modulated in two ways. Explain.
What is Nucleotides? Nucleotides are the basic building blocks of nucleic acids. Each nucleotide is composed of a nitrogen base, a 5-carbon sugar called a pentose, and an ass
Adenosine triphosphate (ATP) is a general form in which the energy is stored in living systems; comprises of a nucleotide (with ribose sugar) with three phosphate groups. Energy c
There are several methods by which a disease-causing organism can be transferred from the reservoir to the host organism. Direct contact : Disease - causing organism may be tr
Define Classification of functions of minerals? The varied functions of minerals can be grouped under four general physiologic roles viz. Structural: They form an integ
What is Endocrine System explain ? The endocrine system consists of glands, organs that secrete hormones. Endocrine glands are tissues that produce secretions within the body,
Q. Can you explain Non-restrictive ventricular septal defect? In non-restrictive VSD PA pressures are elevated hence VSD gradient is low. Non-restrictive VSD is larger in s
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