Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

How infections and infestations cause pem, How Infections and Infestations ...

How Infections and Infestations cause PEM? Childhood infections (viral/bacterial) and parasitic infestations are almost always associated with PEM. These cause anorexia (loss o

Simple closed circulation and double closed circulation, Q. What is the dif...

Q. What is the difference between simple closed circulation and double closed circulation? Double closed circulation or Closed circulation is that in which the blood circulates

Problems with untreated/ammoniated crop residues, Problems with untreated/a...

Problems with untreated/ammoniated crop residues Besides the low energy and protein contents, crop residues generally contain low content of minerals such as Calcium, Phosphor

What do you mean by acute pericarditis, What do you mean by Acute Pericardi...

What do you mean by Acute Pericarditis? Ans. Acute pericarditis is defined as acute inflammation of the pericardium and is clinically characterized by chest pain, fever, t

What is a biologic failure, What is A biologic Failure A biologic Fail...

What is A biologic Failure A biologic Failure can be defined as the inadequacy of the host to establish or to maintain osseointegration. The inability to establish osseoint

Global alteration of brain functioning - sleep, Q. Global alteration of bra...

Q. Global alteration of brain functioning - Sleep? Sleep involves a global alteration of brain functioning, and occupies one third of our lives, The transition from waking to

Genetics, what about cytoplasmic sex determination

what about cytoplasmic sex determination

Explain right atrial enlargement, Q. Explain Right Atrial Enlargement ? ...

Q. Explain Right Atrial Enlargement ? The right atrium (RA) is a globular chamber, forming the right heart border on the PA view. On the PA view, the border of the enlarged RA

What does the formula dpd = sf - tp mean, What does the formula DPD = SF - ...

What does the formula DPD = SF - TP mean? DPD is the abbreviation of diffusion pressure shortage, SF (suction force) is the vacuolar osmotic pressure and TP is the turgor press

Conditions necessary for outbreak in salmonellosis, Q. Conditions Necessary...

Q. Conditions Necessary for Outbreak in salmonellosis? The food must contain or become contaminated with the Salmonella bacteria. These bacteria must be there in considerable n

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd