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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Reagents required and Methodology for Fehling's Test? - Sugar solutions of glucose, fructose, galactose, lactose, maltose, sucrose, starch - Fehling A (Copper sulphat
make a project on heterotrophic nutrition in plants
What are the inheritance and dominance patterns of the Rh blood system? The inheritance pattern of the Rh blood system is autosomal dominant, i.e., the heterozygous manifests a
Explain Food plan for an anaemic pregnant lady? Justification of the Plan: The plan is for an anaemic pregnant lady, from a lower socio-economic level and doing moderate lev
Q. Illustrate Thallium Scintigraphy? It is often done with SPECT (single photon emission computed tomography) which further increases the sensitivity. However, a stress test w
Unsaturated fatty acids show different types of isomerisms. We have already learnt about the.concept of isomerism in the last unit. You would realize that fatty acids with s
Q. Why is the nickname "dark reactions" not completely correct for the chemical stage of photosynthesis? "Dark reactions" is not a correct name for the chemical phase of photos
Q. Properties of foods? various properties of foods, under which the quality attributes of foods were introduced to you in terms of food quality and its categories as appearanc
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Anterior superior alveolar nerve It is a branch of infraorbital nerve which arises within the infraorbital canal. It gives a nasal branch which passes into nasal cavity to supp
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