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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
critera for the animal classification
Differentiate between neuropsychologists and neurologists Cooperation among neuroraudiologists, neurologists, and neuropsychologists has already led to the accomplishment of se
Explain in detail about the Concomitant squint Concomitant squint is also called comitant squint. It means "I follow you", i.e., in all directions of gazes, the squinted eye fo
Explain the Energy Requirements during Sepsis? Patients suffering from septicemia with or without MODS are generally hyper-metabolic which results in weight loss. Critically il
Meaning of Behaviour Change Communication Now day's very common term used for giving health education to people and for giving information to bring change in behaviour is Behav
make a project on heterotrophic nutrition in plants
Q. Why we use Histamine in consciousness? Histamine is involved in controlling the level of consciousness during waking. The level of histamine diminishes during sleep and its
Why Ascending Method of Paper Chromatography is Preferred? The ascending method is preferred because of the simplicity of the set up. In actual method the substance to be separ
Q. What do you understand by Herbarium ethics? You should also know that herbaria provide us with policy statements including such information as 1) filing arrangements, 2) res
uses of conductometry in biological experiments
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