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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Teratology: It is the study of abnormal embryos. In other words teratology is the study of abnormalities of physiological development. It is often use as the study of birth defect
What happens when auxin concentration in some structures of the plant is over the action range of the hormone? In some of parts of the plant (roots, stem, lateral buds) there a
Define Iron Status of the Individual Lastly, iron status of the individual is a floury determinant of how much iron is absorbed. On a mixed diet with some haem iron, the overal
Economic and ecological importance of protozoans
Progressive senescence - Senescence This is the third category of senescence. Here also the leaves are shed but it is gradual senescence of leaves up the stem. For example, th
Radioisotopes Radioisotopes are those forms of an element which exhibit rzdioactivity and so can be used as markers or tracers. For this purpose, we first introduce the radiois
what is a cell
Technique of food processing Freezing is another ancient technique of food processing, which allows the transportation of perishable food items for long distances from producti
What mechanism of regulation is utilized in maintaining homeostatic balance of sugar?
What is the genetic condition in which the heterozygous individual has different phenotype from the homozygous individual? This condition is called lack of dominance and it can
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