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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Explain about biological diversity? Biodiversity or biological diversity is a neologism and portmanteau word, from bio and diversity. It is the diversity of and in living na
Define Principle for Estimation of cholesterol - Lipids? Zlatkis and associates have evolved a method of quantitative determination of cholesterol based on a reagent containing
What is Soil Horizons In a profile, different horizons or layers are identified by certain designations assigned after comparison of the properties of the layer with those o
Explain the Phase Contrast Microscop? Unpigmented and unstained living cells can be easily observed by phase contrast microscope. It has a special objective and a condenser th
Pre-procedural mouth rinse Patients' use of a an anti-microbial mouthwash of 0.12% chlorhexidine gluconate solution for 30 seconds prior to intra-oral procedures will reduce th
CHARACTERSTICS
What are the main biological processes in which calcium participates? Calcium is present in almost all cells and has some functions. Calcium has a significant role in muscul
Explain Voriconazole It has a spectrum of activity similar to itraconazole but appears to be more active against Aspergillus spp. And some species of Candida, containing C.
Q. How are antivenoms produced? Why are antivenoms an example of passive immunization? Antivenoms are obtained by the following process: the venom (antigen) is inoculated into
Explain Glycerol esters of fatty acids Glycerol esters of fatty acids, which make up to 99% of the lipids of plant and animal origin have been traditionally called fats and oi
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