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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
CONSEQUENC E OF OVER POPULATION - With increase in population the available natural resources will fall short of requirements. A severe competition will ensure creating soc
a) In what ways do farmers try to improve the quality of (i) their soil, (ii) their crop plants? b) What other steps do farmers take to maximise the yield from their
Contagious ecthyma The contagious ecthyma virus is immunologically distinct from vaccinia, but similar to the causative agent of pseudo-cow-pox. It belongs to the genus Parapoxvi
Pyruvate Pyruvate has three different fates. Under aerobic conditions, pyruvate enters mitochondriaand is converted to acetyl CoA. The acetyl CoA enters thecitric acid cycle.
Hypothalamus functions are generally aimed at maintaining A region in the brain beneath the thalamus; consists of number of aggregations of nerve cells and controls a variety of a
What are the processes involved in the preparation of plant tissue for free hand sectioning?
Endothelium - Ovule In plants bearing unitegmic ovules, the nucellus degenerates during early stages of ovule development and the embryo sac comes in contact with the innermos
What is the role of Naupliar eye? The unique eye found in larval stage of the crustacean life cycle. This single compound eye is located medially, and with exception of copepod
What is genesis of coronary artery diseases ? Like any other disease conditions, origin of CAD depends on an interplay between the heredity and environment so called 'nature' a
heterotrophic mode nutrition plants
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