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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define the role of riboflavin in Antioxidant Activity? Flavoproteins also have powerful antioxidant activity from their role as precursors to FMN and FAD. Among the FAD-requiri
Q. First type of rheological model? This method establishes relation between the pressure gradient and the volume rate of flow. Here, the piston measurement is used to measure
1) The presence of epicardial coronary artery stenosis caused by artherosclerotic plaques is by far the most frequent angiographic finding in any cardiac ischaemic syndrome. 2)
Explain how a cell produces and releases proteins. Proteins are made on ribosomes and packaged into vesicles by the Golgi apparatus. The vesicles move to the cell membrane and
Q. How does the CD4 counting act to monitor the HIV infection? What is another laboratory method to follow up the disease? The CD4 counting test is complete from a blood sample
Plants are supplied with adequate amount of phosphorus Phosphorus has also been associated with early maturity of crops particularly the cereals. Its shortage is accompanied by
Explain about the plateau effect - Energy Balance? You must have noticed that when people start following weight reducing diets, they lose weight rapidly in the beginning, then
How many carbon dioxide molecules are liberated after each cycle of the Krebs cycle? For a single glucose how many carbon dioxide molecules were already liberated by the aerobic re
Q. Procedures Adopted by Numerical Taxonomists? Since numerical taxonomy is an operational science, the procedure is divided into a number of repeatable steps, allowing the res
Q. What are alkalosis and acidosis? Acidosis is the condition in which the blood pH is abnormally low Alkalosis is the condition in which the blood pH is abnormally high Normal
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