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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
mode of digestion in coelenterata
What is the function of the skin in humans? The skin is the external covering of the body. In humans its major functions are protection, perception of information from the envi
what is cell?
Are fruits always the flesh part of the "fruits"? Is the edible part of the onion a fruit? In some so-called fruits the actual fruit is not the flesh part. For instance, the fl
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Types of Forests - Ecosystem The forest biomes of the world have been classified into the three major categories: coniferous forest, tropical forest and temperate forest. C
Sinus Perforations The maxillary antrum can sometimes be inadvertently penetrated. Through careful pre-operative planning using radiographs, including axial scans, this compli
Water Pollution Water is one of the most essential requirements for the survival of all living organisms. Water is used for household, agricultural, industrial and recreation
It is found in around 20 per cent cases of ARF and it is a late manifestation occurring even 3 months after GAS pharyngeal infection. Chorea is triggered by emotional disturbances
what do our brain and heart do when we are asleep?
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