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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Theory or Principle of Determination of Coliforms? Coliforms are gram negative, non-spore forming, facultative anaerobic bacteria which produce acid and gas on lactose
Which of the following require ATP? The movement of potassium from the outside of the neuron to the inside? The movement of sodium into the neuron through a chemically gated
Name the various suturing techniques There are a various suturing techniques each suited for a particular situation. Few of the common suturing techniques are mentioned here
Mineralisation and Humification As a result of physical weathering, the rocks are broken down into smaller particles. But this is not the true soil, and plants cannot grow well
Plankton - Aquatic Ecosystem This group includes both microscopic plants (phytoplankton) and animals (zooplankton) found in all aquatic ecosystems, except certain swift moving
Explain about Suspensions Sol is a colloidal system, in which solid particles are dispersed in a liquid. When the particles of a solid are separated into large aggregates of pa
Hybridization is the reaction by which pairing of the complementary strands of nucleic acid happens. DNA is usually double-stranded, and when these strands are separated form each
Why does thermal inversion increases air pollution? What harm can thermal inversion cause to humans? Thermal inversion confines at low altitude a layer of pollutants that would
Q. List the areas of educating a diabetic patient? 1 Self monitoring of Blood -glucose 2 Urine testing 3 Hypoglycaemia 4 Self Administration of Insulin 5 Foot Care
What are the causes of obesity - Etiology? However simple the question may sound, the answer to it is not all that simple. We cannot deny that excess weight results from positi
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