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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What happens when the ovum is fertilized? If the ovum is fertilized, there is no breakdown of endometrium and no menstrual flow. The fertilized ovum travels through uterine tub
Q. Describe Postpartum Cardiomyopathy? It is defined as presentation of LV Systolic dysfunction and heart failure in last trimester of pregnancy or within 6 months of delivery.
Explain the Life History of Lycophytes? Lycophytes have two separate and distinct generations in their life history that alternate with each other. The gametophyte stage is tin
Concerning their biological function what is the difference between mitosis and meiosis? The major biological function of mitosis is cellular multiplication, a fundamental proc
ENERG Y TRANSFORMATION - During photosynthesis radiant energy is converted into chemical energy by green plants. In biluminiscent organisms .e.g glow worm, noctilluca &
Give three ways in which a plant might be adapted to a hot dry environment. A plant might be adapted to a hot dry environment by having a decreased leaf area, waxy cuticle to
Explain Cis-trans isomers Atoms or groups are called cis or trans to one another when they project respectively on the same or on opposite sides of a reference plane identi
Explain Indications for Surgery in Aortic Aneurysm? Indications for Surgery : Thoracic aortic aneurysms are classified by the portion of aorta involved - ascending, arch,
The clinical manifestations of IE result from the local destructive effects of intracardiac infection; the embolization of bland or septic fragments of vegetations to
What are the three main types of trophic pyramids studied in Ecology? The three kinds of trophic pyramids studied in Ecology are the numeric pyramid, the biomass pyramid and th
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