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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Brain Neurosecretory Cells and their Hormones Kopec was the very first to suggest the role of hormones in controlling metamorphosis. On the base of his experiments on the lar
Clothes : The concept of clothes might have started even before weaving, as an extension of the practice of carrying food hd implements about. Attachments with a convenient
Q Which are the extra abundant ribosomes in secretory cells - the free cytoplasmic ribosomes or those associated with the rough endoplasmic reticulum? Free cytoplasmic ribosome
Cellular Transport Cellular transports demote to the movement of compounds across the outer wall or membrane of the cell. This transport is critical in two respects. Firstly, t
Define the effect of niacin deficiency on Nervous System? Delirium is the most common -mental disturbance in acute pellagra. Dementia is more frequently seen in the chronic cas
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Describe the Methods of Assessment The four general principles outlined above, that is, adaptation, brain and behaviour, context, and development serve as the foundation for th
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Define Isotope Dilution Method? The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance i
Q. Define Eye Balling? Ans. Two-dimensional echocardiography provides a good visual perception of cardiac functions. With experience, the echocardiologist learns to percei
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