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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
The cell membrane is a slim semi-permeable membrane which surroundings the cytoplasm of a cell. Membrane function is to defend the integrity of the interior of the cell by allowing
Q. What is the lymphatic system? The lymphatic system is a network of specialized valved vessels that drain interstitial fluid (lymph), the lymphatic system is as well responsi
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What is the difference between asexual and sexual reproduction
Platinum color in foxes is produced by heterozygous genotype Ss, and silver foxes are the genotype SS. The genotype ss is lethal, and the fox dies in early embryonic development. I
right classification of protozoa up to class
Discuss six bad listening habits. Which do you think is the biggest challenge for you personally?
Ethylene glycol is a poison that causes about fifty deaths a year in the United States. Treating people who have drunk ethylene glycol with massive doses of ethanol can save their
a) Explain the polygonum type of embryo sac. Why is it generally referred to as monosporic ? b) Explain human leucocyte antigen complex? Describe its role in organ transplantati
is the leukamia dangerous
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