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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is predatism? The Predatism is the ecological interaction in which one individual kills or mutilates another to get food. The Predatism is an inharmonious (negative) ec
Why does one need to study about soil and what is its importance? You would be aware that, from the dawn of agriculture, cultivators were attracted to fertile soils of river
TYPES OF CELL WALL Cell wall differentiated into the following layers : 1. Primary Cell Wall 2. Secondary Cell wall 3. Tertiary Cell wall 1 .
Q. What is the valve that separates the right ventricle from the pulmonary artery? Why is that valve important? The pulmonary valve is important to prevent blood from the pulmo
Young's Trichromatic Theory According to Young's theory, three types of cones exist, each sensitive to a particular pigment-rythrolabe (red), chlorolabe (green), and cyanolabe
Explain the relationship between an agonist muscle and its antagonist as it relates to positional control, functional movement and neuromuscular activation. For example, how the tw
Success which signifies Optimum Health The features or clinical conditions which are representative of this group are: i) No pain or tenderness in the implant upon function.
How body fat can be measured? The conventional golden method of measuring BF% is by underwater weighing. Difference of weight in air and in water gives density, from which the
During the life cycle of most animals, some diploid cells undergo meiosis and haploid form in a process called gametogenesis. Spermatogenesis: In the testes of the male, the
Define osseointegration and its theories. Osseointegration implies that "it is a contact established without interposition of non bony tissue between normal remodeled bone and
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