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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain about the optic disc The optic disc is also called a blind spot while the macula is referred to as the yellow spot. The ora serrata (the intersection between the retina
How are antivenoms produced? Why are antivenoms an example of passive immunization? Antivenoms are getting by the following process: the venom (antigen) is inoculated into othe
WHY PROTOXYLEM LACK TYLOSIS?
Which type of cell came first in evolution the eukaryotic cell or the prokaryotic cell? Ans) This is an interesting problem of biological evolution. The most accepted hypothesis
Define Amino acid requirements for Human? Data regarding the essential amino acid requirements of infants, children and adults are given in terms of egg protein and cow's milk
polyspermic fertilization
Define Stress Factors - Etiological Risk Factors in Cancer? Emotions playing a part in malignancy are not a new idea. But these relationships are extremely difficult to measure
DEMOGRAPHIC CYCLE - 1 . First stage - B high - D high = 0 2 . Second stage - B high - D lo w = G more eg.
Stage two using a mucoperiosteal flap A Mid crestal incision over the implant site is made and reflection of mucoperisteal flap is done under local anesthesia. After locating t
BLOO D CLOTTING - It is a nature's device to check the excessive loss of blood from an injury. Bleeding time is 1-3 minutes. Clotting time is 2-6 minutes. Process of
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