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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What are the Pseudo-yeasts? These are like true yeasts but do not form spores. All the members of this group are particularly unsuitable for fermentation purposes as they p
How has the position of the stomata changed in some plants to prevent excessive water loss by transpiration? In the some plants that have leaves that receive too much sunlight
EGG S OF HEN It is a meiolecithal egg which is oval. The animal pole is found in the form of a small germinal disc. The vegital pole has two types of yolk viz. White yol
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what are the negative and positive economic importance of snai
Retrograde Peri-Implantitis It has been described by Misch as implant failure probably due to bone microfractures caused by premature implant loading or overloading, other form
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Determine the Occurrence of vitamin K Vitamin K is found naturally in plants and vitamin K2 is synthesized by the bacteria in the intestines in humans. In nature, only two nap
The first evidence of implants dates back to 600 A.D. in the Mayan population. There are reports that the ancient Egyptians attempted intra-osseous implantation of animal teeth or
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