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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Explain Bruce Protocol? Bicycle Ergometry Most bicycle test are performed sitting upright, but supine bicycle test have become more popular. Treadmill Test The
Coarse fish reach or lowland course zone This zone corresponds to the lower course of the river. Here the river is deep and slow moving. Its sluggish flow results in the depos
How is the genetic determination of sex established in humans? In the diploid genome of the human beings, there are 46 chromosomes, 44 of them are autosomes and two are sex chr
Phylogenetic considerations
a) Mention the scientific term used for modified form of reproduction in which seeds are produced without fusion of gametes. b) What does ecological niche of an organism represe
Define Manganese Metabolism - Micro Minerals? Intestinal absorption of Mil occurs throughout the length of the small intestine although the exact mechanism of absorption is not
Explain the histologic techniques A clinician has to base the diagnosis only on the clinical and radiographic findings as microbiologic and histologic techniques are restricte
A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction
Q. After the fact that it is based on information from mRNA what is the process of protein synthesis called? Protein synthesis is called translation of genetic information into
SQL's Fifth Concept - NULL Unfortunately, SQL embraces a fifth concept, called NULL, an apparently simple little thing but one that has pervasive effects on our usual underst
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