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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Most dental offices have a designated area for instrument reprocessing that is separate from the dental treatment room. This is ideal, since cleaning, sterilizing and storing instr
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Why is it important to be familiar with the laboratory apparatus and their uses? If you do not use instruments or lab apparatuses correctly (or use an apparatus for something i
what are the theories of animal classification
Outline the steps involved in using bacteria to produce human insulin. The gene for insulin is 'cut' from the appropriate strand of DNA using restriction enzymes. Plasmids are
What are the Advanced surgical procedures The scope of implantology has widened to include many more clinical situations which would be hitherto contraindicated for implants du
interpretation of liver photomicrograh slides
Cardiomyoplasty This is a procedure wherein the latissimus dorsi muscle is wrapped around the heart and stimulated to contract synchronously with it. It has been largely given
Proteins of Animal Origin - Milk: Milk: A value of 3.5% protein is often considered as an average for milk. Milk protein has traditionally been divided into 2 classes - casei
What are the parasympathetic neurons Patient A has a disease that has destroyed half of the alpha-adrenergic receptors on each of the smooth muscles that control the diameter o
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