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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Is a gene the same as a DNA molecule? Genes are units of heredity. A gene is a section or segment of a DNA molecule. This section of DNA provides a genetic code for synthesi
Q. How is the striped pattern of the striated muscle cells formed? The functional units of the muscle fibers are the sarcomeres within the sarcomeres blocks of myosin and actin
Q. What are the "chiasms" of homologous chromosomes observe in prophase I? Chiasms are intersections of two tracts in the form of X, The chiasms seen in prophase I are chromoso
summarise the comparative anatomy of the digestive system of the invertebrate and the vertebrate.
Mass Dispersal - Population Dispersal A population is inherently dynamic in nature since individuals are always leaving or entering the populations. But such changes normally
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Unprecedental clarity has come to our understanding of genetic differences by the analysis of DNA sequences. It is not surprising in which the latest DNA technologies are leading t
what is a scientific name of mango?
Explain the Bioavailability of Nicotinic Acid? We have already learnt earlier that niacin is provided in the diet primarily as the pyridine nucleotides-NAD and NADP. In additio
Explain the Periapical surgery - Endodontic Surgery a) Curretage 1 b) Root-end ressection 2 c) Root-end preparation 3 d) Root-end filling 4
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