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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is low - density lipoprotein receptor pathway? Ans. The increasing cellular free cholesterol generated regulates the activities of two enzymes that are of crucial
Determine Materials for Separation of Amino Acids by Paper Chromatography? Petridishes (200 mm diameter) with lid, Whatman Chromatographic paper, brown paper, 500 ml separating
what are some physical features of the ocean
Bovine spongiform encephalopathy The bovine transmissible spongiform encephalopathy (BSE), known as 'mad cow disease'-first noticed in Great Britain in 1986, is similar to scra
scientific names of animals
Types of suture materials The suture technique and material selection should be based on a knowledge of the desired goals of the respective surgical procedures and the physical
Using Autonomous Transactions The transaction is a sequence of SQL statements that does a logical unit of work. Frequently, one transaction starts the other. In several appl
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The kind of epithelium which forms the inner walls of blood vessels is : 1. cuboidal epithelium 2. columnar epithelium 3. ciliated columnar epithelium 4. squamous epith
Explain about the Pickling - methods of food processing? Pickling was widely used to preserve meats, fruits and vegetables in the past, but today it is used almost exclusively
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