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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How are lipids classified?
explain the role of cyclic photophosphorylation in photosynthesis
VERTEBR A T E HEART Heart = Cardia. Most important organ of body. Main function is to pump the blood. Study of heart is cardiology. In heart auricles and ventricles pr
Excretory organ of lizard.
Ooplasmic Determinants and Somatic Cell Determination Eggs of tunicates go through typically mosaic development in which the various blastomeres become determined to follow th
Explain Nephrotoxicity Nephrotoxicity is the major dose-limiting toxicity of amphotericin B deoxycholate; sodium loading with normal saline may prevent or ameliorate it and is
How sugar is used in Icings? Sugars provide sweetness, flavor, bulk and structure to icings and frostings. In addition to sweetness and flavor, icings also function as a barrie
Explain the types of Carrageenan It was first produced commercially from the red algae, Chandrus crispus, found along the northeast shores of the U.S and Canada and referred t
Explain arterial continuous mummer? Arterial Continuous Mummer : These originate in constricted arteries as in carotid or femoral artery obstruction (Mummer are louder in sys
NON-MUSCULA R MOVEMENT - 1 . Streaming movement - In amoeba, cyclosis is common. 2 . Pseudopodial - In leucocyte, macrophages, amoeboid movement take place
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