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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Tricuspid Annuloplasty : Tricuspid valve is evaluated by digital palpation before connecting the patient to cardio pulmonary by pass. Functional regurgitation can be repaire
An ecosystem includes
Q. How do arthropods grow? Due to the presence of exoskeleton the growth of an arthropod is periodical during the growth period the animal loses the exoskeleton, develops and g
Tetanus This is an infectious, non-febrile disease of animals and man, and is characterised by spasmodic tetany and hyperaesthesia. The causative agent is Clostridium tetani,
What is the difference between osmosis and diffusion? Osmosis is the occurrence of movement of solvent particles (in general, water) from a region of lower solute concentration
Define Brain Stem Brain stem consists of three parts i.e. midbrain, pons and medulla oblongata. The functions of the Brain Stem are: Brain stem contains points of cranial
Which of the following is a false statement regarding restriction enzymes? A. Restriction enzymes bind to a sequence specific element and disrupt the hydrogen bonds among
Metabolic Disorders The disease conditions, which are attributable to an imbalance between rates of input of dietary nutrients and the output of production, are defined as pro
Why does oxygen concentration in the blood decrease during exercise? Why does carbon dioxide concentration in the blood change during exercise?
Describe Valsalva Manocuvre in dynamic auscultation ? This consists of deep inspiration followed by forced inhalation against a closed glottis for 10-20 seconds. It can be perf
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