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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
In the evolution of whales, there is evidence that whales and hippo form a monophyletic group. Show all the evidence that supports the close relationship between whales and hippos
What is ADP phosphorylation? What respectively are photophosphorylation and oxidative phosphorylation? ADP phosphorylation is the addition of one inorganic phosphate in the mol
Explain the Energy requirement in Postoperative Nutritional Care? Adequate calorie intake is critical for successful outcome of surgery and should be provided in the form of ca
Define Limitations for Standard or Total Plate Counts Method? This method has certain limitations - 1. Microbial count depends on the type of media, incubation temperature,
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what is almentary canal
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State three main ways in which the body uses food. The body uses food (i) for energy, (ii) for growth (making new cells), (iii) repairing or replacing tissues.
1 list the names of the phyla of animalike protists that have only free-living members
DEALCOHOLISM - Treatment of alcoholism or withdrawal symptoms of alcohol is known as dealcoholism. Recovery from alcohol dependence is greatly aided by sociobehavioural c
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