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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
classification sketch of protozoa
What are the two divisions of meiosis? What are the main events that happen in those divisions? Meiosis is divided into first meiotic division, or meiosis I, and second meiotic
Define the Energy requirement to avoid Underweight problem? The total calorie intake should be 500 to 1000 Kcal in excess of the daily needs in order to result a gain in weight
At 1 AM, an impermeable membrane separates a 1 liter solution of 2M KCl in the left compartment from a 1 liter solution containing both 1M NaCl and 1M KCl in the right compartment.
Q. Why is maternal milk important for the immune protection of the baby? Besides being nutritionally important like maternal milk participates in the defense of the baby agains
Fibroids, also called as leiomyomas, are benign smooth muscle tumors that happen in the uterus. They grow under the influence of estrogen and progesterone, and are therefore seen i
Q. Can Obesity causes the cardiovascular disease? As you know obesity or excessive weight is the primary cause of cardiovascular disease. It is an independent risk factor for h
During a routine annual physical, a patient was checked to determine the amount of glucose in the blood. After the assay, It was found that the glucose that the glucose concentrati
Packaging Compound feeds, whether in mash or pellet form, are packed in bags or stored in bins. Bags may be filled directly from mixers, pellet coolers or holding bins and wei
Q. What is Coping? Coping refers to a person's patterns of response to stress. Coping is the process of managing circumstances, making effort to solve personal and interpersona
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