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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
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FUNCTIONS OF E.R. (1 ) Mechanical support - E.R. participates in formation of cytoskeleton with microfilaments, microtubules and intermediate filaments. (2 )
Why is the fish circulation classified as a simple and complete circulation? Complete circulation is that in which there is no mixture of venous blood and arterial blood. As ci
Name four substances that have to be excreted from the body. Substances that have to be excreted from the body are:- A) Carbon dioxide, B) urea, C) uric acid, D) s
Define the Standard Deviation (2 score) classification? Statistics teaches us that when normal values of any variable are distributed as per their frequency of occurrence, they
what is taxonomy
Enumerate different implant materials with their pros and cons Biomaterials fall into four categories: metal and metal alloys, ceramics (carbon included in this group) syntheti
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What is a goiter? What is endemic goiter? How is this problem socially solved? Goiter is the abnormal enlargement of the thyroid gland. The goiter appears as a tumor in the ant
Chromophore - Development of plant The chromophore is a tetrapyrrole molecule like chlorophyll, but unlike chlorophyll it is an open tetrapyrrole and contains no metal ion. It
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