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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is the mode of nutrition in fish,human,amoeba,scorpian & toad ?
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What are mineral salts? Where in living beings can mineral salts are found? Mineral salts are simple inorganic substances made of metallic chemical elements, such as iron, sodi
Q. What are the major human diseases caused by virus? Among diseases caused by virus are common cold, variola (considered eradicated nowadays), mumps, measles, rubella, AIDS, t
There is a wide spectrum of potential clinical presentations with heart failure. Most patients have signs and symptoms of pulmonary congestion including dyspnea, orthopnea, and par
There is no store for nitrogen-having compounds as there is for carbohydrate (glycogen) or lipids (triacylglycerol). Thus nitrogen ingested in excess of what is required through
If an enzyme-controlled reaction normally takes place at 10ºC, in general terms how will the reaction be affected by (a) a fall in temperature to 2°C , (b) a rise in temperature t
The Eukaryotic transcription is more difficult than prokaryotic transcription. For example, in eukaryotes the genetic material DNA and therefore transcription is basically localize
Is the effect of genetic drift likely to be the same in pop 1 and pop 2? How are genetic drift and pop size related? when there is strong selection against the homozygous recessive
Determine about the term - heterogeneous mixture The solid phase made up of soil and other source material for nutrients, is a heterogeneous mixture. Each nutrient source contr
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