Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Testis of non-mammalian vertebrates, Normal 0 false false f...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Find initial osmotic pressure on room temperature of a cell, Find the initi...

Find the initial osmotic pressure at room temperature of a cell if the only ions present are CaCl2 on either side of the membrane. Assume the concentrastions for K+ and Cl- from Ta

Hemoglobin, describe the mechanism of allosteric change.

describe the mechanism of allosteric change.

Functions of endoplasmic reticulum, FUNCTIONS OF E.R. (1 )      Mecha...

FUNCTIONS OF E.R. (1 )      Mechanical support - E.R. participates in formation of cytoskeleton with microfilaments, microtubules and intermediate filaments. (2 )

How does the absence of a nuclear envelope in prokaryotes, How does the abs...

How does the absence of a nuclear envelope in prokaryotes prevent prokaryotes from controlling gene expression by modifying RNA after transcription? Without a nuclear envelope

What is defective colour vision, What is Defective Colour Vision Defect...

What is Defective Colour Vision Defective colour vision is often called colour blindness. The ability to appreciate one or more of the primary colours is lacking. This can be e

Explain pasteurization, Explain Pasteurization The act or process of h...

Explain Pasteurization The act or process of heating a beverage or other food, to a specific temperature for a specific period of time in order to kill microorganisms that cou

Therapeutic diets - qualitative methods, Therapeutic diets - Qualitative me...

Therapeutic diets - Qualitative methods Qualitative methods: This  is where  you  give  the individual choices,  clear guidelines, menu guidance and supporting  information  su

Theca - body form protozoans, Theca - body form Protozoans It is a clo...

Theca - body form Protozoans It is a close-fitting hard structure around the plasma membrane, mainly composed of cellulose. It can be compared with the ceil wall of a plant ce

How execute the two complementary nucleotide chains, Q. How execute the two...

Q. How execute the two complementary nucleotide chains of the DNA facilitate the replication process of the molecule? The fact that DNA molecule is made of two polynucleotide c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd