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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What volume of 4x protein sample buffer do you need to add to 15 ml of a cell extract in order to load it on a gel?
The human blood group, related to the MN system is controlled by two alleles, S and s. Three distinct phenotypes can be identified by mean of biochemical tests. Among 1000 Britishe
What er the Parafunctional Habits Parafunctional Habits like bruxism and clenching create mechanical and biological problems due to overloading and are considered to be one of
G L YCOLIPIDS They are lipids having sugar residues. Two common glycolipids are cerebrosides and gangliosides. (1) Cerebrosides They are made of amino alcohol sphi
WHAT IS CHARACTER SELECTION CRITERIA
Q. How do chordates reproduce? Reproduction in beings of the phylum Chordata is sexual, with the exception of urochordates that can be also reproduce asexually. In some classes
Please help with the following: For each of the four macromolecules carbs, lipids, proteins and nucleic acids, please identify the monomer or molecular components and name the b
Explain Changing Body Composition with Ageing? With ageing, a progressive decline in the water content and the lean body mass is accompanied by an increasing proportion of body
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Q. In which period of meiosis does the pairing of homologous chromosomes occur? The pairing of homologous chromosomes is a very important step for meiosis because the rightness
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