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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What are some fungal diseases transmitted by animal feces? Pigeon and Bat feces can carry Histoplasma capsulatum, the fungus agent of histoplasmosis. The infection is transm
Open Pulmonary Valvotomy : Open pulmonary valvotomy by technique of inflow occlusion is done without cardio pulmonary bypass. Surface hypothermia is used and inflow occlusio
What is protein denaturation? Is there any change in the primary structure when a protein is denatured? Secondary, tertiary and quaternary structures of proteins are spatial st
Q. Objective of nutritional management of hypertension? The objective of nutritional management of hypertension includes: • To achieve gradual weight loss in overweight and
Migration - Population Dispersal Dispersal is affected by the presence or absence of the barrier and vagility which means inherent power of movement also called dispersal powe
Loading and Unloading of Sieve Tubes In order to understand the loading of food from manufacturing leaf cells to sieve tubes we must examine the anatomy of a minor vein shown
Disorder of Platelets - Purpura Purpuras are bleeding disorders characterised by petechiae and ecchymosis which may be due to deficiency in either, the number or quality of
Degradation of Ecosystem Ancient man was simple-minded food gatherer and hunter. He looked upon nature with awe and respect and in fact he worshipped it. But from the time he
What are the cytokinins? And where are they made? The Cytokinins are phytohormones active in the promotion of cellular division they slow down the aging of tissues and act toge
Q. What is the use of Adrenergic Receptor? Adrenergic Receptors are also subdivided into 2 major categories: ALPHA α - or- BETA β In general, when NE (E) binds to alpha r
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