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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is the optimum temperature for catalyses? For any chemical reaction, the reaction rate enhances with temperature, so the higher the temperature, the faster the rate. For a
Class adverse effects of NNRTI All NNRTIs can cause a rash that is sometimes severe. NNRTIs are metabolized by and can affect hepatic CYP 450 isozymes; drug interactions can o
Q. What is the formula of the net primary production (NPP)? How does NPP relate to the energy pyramids? The Net primary production is the gross primary productivity less the or
(a) Anti-malarial drugs kill the parasites in the blood but they do not cure the disease.Why is this? (b) Why was the use of DDT insecticide not successful in eradicating mal
What is the probability of two individuals (AaBBccDd and aaBbCcDd) having a child with the following genotype AaBBCcDD?
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The xylem and phloem of the plant could be described as the "circulatory system" of the plant. However there are key differences between vascular tissue in plants and our own circu
How plants solve the problem of transporting substances throughout their tissues? In the bryophytes the substance transport is done by the diffusion. In the tracheophytes (pter
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Alluvial Materials of this catego~ya re deposited by running water in the form offroodplains, river terraces, deltas and alluvial fans. These deposits have two outstanding char
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