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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is Echocardiography? Ans. Echocardiography has become an established and powerful tool for diagnosing presence of CAD and defining its consequence. It can help in
How Water content Influence the RS content? The yield of RS formed in heat-moisture treatment is closely related to water content, which may be an inherent component of food or
Q. Can solutions with the same concentration of diverse solutes have different osmotic pressures? The osmotic pressure of a solution does not depend on the nature of the solute
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Describe how many bones are there in human foot The human foot is a great mechanical marvel. It has 26 bones, 29 joints, and 42, various ligaments, a sensitive and protective s
are protozoans primitive to life .
Define Basic Properties To Proteins, To Be a Good Foaming Agent? A protein must: a) Be able to rapidly absorb at the air-water interface during whipping, b) Undergo rapid
Explain Procedure for the use of Light Microscope? Now carry out the exercise following the steps enumerated herewith. 1. Place the microscopic slide with any specimen on th
Alternative polyadenylation sites Exact pre-mRNAs hold more than single group of signal sequences for 3' end polyadenylation and cleavage. In some cases, the area of t
What are the classes in Phylum coelenterata?
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