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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Objective of nutrient needs during periods of pregnancy? describe the various physiological changes during pregnancy, describe foetal growth and development and
Explain Sodium Sodium (Nu) : In sodium-restricted diets, no salt is added to the diet which still provides approximately 50 mmoL Na. Foods containing high Na content must b
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IMPORTANCE OF MENDELISM 1 . Improvement of Plants Hybridization is used for obtaining improved varieties of plants. This process results in combinations of desirable
Q. Write the meaning of Polydypsia? Increased Thirst (Polydypsia): As the patient is losing more water through excessive urination, the patient feels thirstier and tends to tak
Q. Why does the urinary volume increase when alcoholic beverages are ingested? Alcohol inhibits the ADH (antidiuretic hormone) secretion by the hypophysis and Low ADH reduces t
Benthos - Aquatic Ecosystem The benthos or the benthic organisms are those found living in or on the bottom or benthic region of the water mass. They exhibit a variety of adap
Define Nutritional Management of Eating Disorders? In the last unit, we focused on weight management. It is important to understand that the problems associated with too much d
Define Excretion of Methyl malonic acid in urine (Methyl malonil aciduria)? Methyl malonic acid is found only in traces in normal urine (1-2 mg/day). In vitamin B 12 deficie
Armamentarium 1. Edentulous stock trays 2. Rubber bowl and alginate mixing spatula, plaster mixing spatula 3. Vernier calipers 4. Alginate impression material 5. Gy
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