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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Initiation Codon is the codon at which the translation of a polypeptide chain is initiated. This is generally the first AUG triplet in the mRNA molecule from 5' end, where the rib
what is respiration of plants and its types
How does the excretory system of nematodes work? The metabolic residuals of nematodes are collected by two longitudinal lateral excretory channels that open in one excretory po
Causes of Degradation of Ecosystem Due to Agriculture Degradation of the ecosystem due to agriculture can be on the following accounts: a) Removal of trees (deforestation
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Define some Essential facts about the Fats? 1) Fats are essential in diets to facilitate satiety, high-energy intakes, and absorption of fat-soluble vitamins and provide essent
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Define Streptomycin Streptomycin causes ototoxicity (usually vestibular disturbance) and, less frequently, renal toxicity. Amikacin and kanamycin commonly cause tinnitus an
Thickness and toughness of plant walls are increased by deposition within original cellulose wall, of a substance called as lignin. Process of deposition is called: a) Lignific
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