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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Glycogenesis : This is an anabolic process in which glucose is polymerized into glycogen by the sequence of reactions. Since glycogenesis occur in all body cells .but
Which of the following best describes why Okazaki fragments are not observed in PCR? A. DNA primase is used for the entire reaction therefore eliminating the production of Okaz
Do fishes there an open or closed circulatory system? How many chambers does a fish heart have? How does blood flow all through the fish body? As in every vertebrate the circul
Define Intra enterocyte transport - non-haem iron absorption? Intra enterocyte transport: In the enterocyte, the absorbed iron can have one of the following metabolic fates:
1. DNA damaga can be spontaneous or can be 'Induced by external agents a. What are 'spontaneous' mutations? Give examples of causes of spontaneous mutations and the kinds od DNA
Define the LTLT Pasteurization Method? In LTLT pasteurization, the pasteurization time is in the order of minutes and related to the temperature used; two typical temperature/t
PREPARATION FOR RESUSCITATION Two trained, personnel capable of working together to perform all aspects of resuscitation, it may be nurse and paediatrician or two skilled nurs
Q. Define Premotor Cortex Located in the frontal lobes? Premotor Cortex - Involved in control of more complex, learned motor skills like writing, driving, etc. Broca's area or
assignment of digestive gland
Q. Explain Pulmonary Capillary Wedge Pressure? The PCW waveform is similar to LA pressure waveform except that it is damped and delayed due to transmission through the lungs. T
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