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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain some side effects of carbohydrate loading? Some potential side effects of carbohydrate loading are: Muscle , stiffness Diarrhoea Chest Pain Depres
Explain Blood Vessels that carry blood? Blood vessels that carry blood away from the heart to the lungs or to the rest of the body are called arteries. The walls of arteries ha
Q. Why is carbon monoxide toxic for humans? Hemoglobin "likes" carbon monoxide (CO) much more than it likes oxygen. When there is carbon monoxide in the inhaled air it binds to
Q. In the long term which are the organs affected by chronic Chagas' disease? In the chronic stage of Chagas' disease, that manifests years after the infection, the trypanosoma
Explain briefly how the stimulus at the finger produces an impulse in the sensory neurone using the following key words. 1. Energy conversion (linked to receptor) 2. Na+ in 3. Depo
What do numeric pyramids represent? Numeric pyramids show the number of individuals in each trophic level of a food chain.
Symptoms of digitalis toxicity include anorexia, nausea, headache, blurring or yellowing of vision, and disorientation. Cardiac toxicity may take the form of atrioventricular condu
Late recurrence of artgirza: This is a reflection of progress of disease in the native coronary arteries distal to the grafts or narrowing or blockage of one or more of the g
describe food procurement and digestion in ciliates.
What is the other name given to sex chromosomes? What is the function of sex chromosomes? Sex chromosomes are also known as allosomes (the other chromosomes that are not sex ch
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