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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
vasoconstriction
What is PCR? How does PCR works? PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor
Which of the following is a false statement regarding prokaryotic and eukaryotic core promoters? A. The core promoters of eukaryotic genes have more recognition elements for ge
explain pcr
Shoot Multiplication -Clonal Propagation This is the most important step with respect to the rate of propagation and genetic uniformity of the product. The most reliable and
What is visual accommodation? Visual accommodation is the phenomenon of varying the curvature of the crystalline lens to make possible the variation of its refractivity to adju
Q Are bacteria the only prokaryotic beings? Prokaryotic beings are classified into two big groups: bacteria and archaebacteria (this last also known as eubacteria). Compared
What are the dietitians services Dietitians are engaged in a variety of positions and in a number of work settings. Of course the largest proportion is involved engaged in
Q. What is Phytates? Phytates are widely distributed in seeds of vegetables, fruits and grains. Unrefined cereals and millets are the richest sources of phytates. Refined or
Ac ute heart failure The acute heart failure is characterized by sudden loss of consciousness and falling with or without convulsions. The mucous membranes become pale followe
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