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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define the Coenzyme activity of nicotinic acid? Nicotinamide exists within the redox-active coenzymes, nicotinamide adenine dinucleotide (NAD) and its phosphate (NADP), which
Agarose gel electrophoresis is the method to analyze the size of the DNA (or RNA) fragments. In the presence of an electric field, bigger fragments of DNA move through a gel slowe
Explain disease typhoid Typhoid is often called enteric fever because the infection or bacteria is found in the intestines and attaches itself to the epithelium of the intes
DIANCEPHALO N - Less visible. Occupies only 1% of brain's volume. Its lumen is diocoel or III ventricle. Roof is epithalamus . On it pineal body is present. Fl
what is the chemical reaction of respiration in cockroach?
Q. In general what are the products and reagents of fermentation? In fermentation glucose sugar is degraded into pyruvic acid each glucose molecule forms two pyruvic acid molec
Q. What is population density? The Population density is the relation between the number of individuals of a population and the area or volume they occupy. For instance, in 200
Lipids are essential fats that have much importance to the human body. Lipids are biological molecules that are insoluble in aqueous solutions and soluble in organic solvents are c
Describe the access in heart disease Newborn? A secure peripheral or central intravenous access is very essential. Inotropic agents with vasoconstrictor properties like Dopamin
Explain the term Lipids? If somebody discovers that you have some knowledge of nutrition, you will be promptly shooted with two questions, first, "Oh! You really can tell how m
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