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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Definition of a neuropsychological test The best definition of a neuropsychological test has been offered by Ralph Reitan, who described it as a test that is sensitive to the
Transferring the Patient form ICU to Ward The patient is made to ambulate in ICU before shifting to ward . vitals are stabilized. The ward nurse is explained the pa
Explain the Determination of Hydrolysis Hydrolysis of calcium pantothenate in acidic medium produces α,γ-dihydroxy-β,β-dimethylbutyrolactone, which reacts with hydroxylamine i
Explain Deteriorative changes in fats and oils The food products undergo changes in flavour due to the chemical changes occurring in fats and oils present in them. The causativ
PHYLUM ARTHROPODA Definition and Introduction Bilateral and protostomial eucoelomate eumetazoa with metamerically segmented and each segment bearing a pair of join
Q. What is a mixed gland? Why the pancreas is considered a mixed gland? Mixed gland is a gland that produces exocrine and endocrine secretions. The pancreas is an example of
Explain Precautions for Estimation of Sucrose by Fehling Soxhlet Method? 1. Maintain proper temperature of the water bath for complete hydrolysis. 2. Add distilled water to
Explain the Digestive System ? The digestive system takes in food and processes it. Digestion is the mechanical and chemical breaking down of food into a form that can be used
Define Types of Root Canal Perforations According to location According to location (level) in relation to crestal bone): Coronal perforation: (supragingival) coronal to
Oceanic islands are interesting and somewhat specialised biomes. Islands which have broken away from the main continents have similarity of flora and fauna related to the continent
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