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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Mosaic and Regulative Eggs Eggs as well as early cleavage embryos are occasionally divided into two categories: i) Mosaic or determinate embryos and ii) Regulative or i
Ruminate Endosperm - Variants of Endosperm In certain plants the surface of the mature cellular endosperm shows a high degree of irregularity and unevenness, giving a ruminate
Define observation or inference for barfoed test? 1. Reddish brown precipitate is seen on the sides and bottom of the tube. The precipitate of the sides and bottom indicates th
Q. Explain the factors on which Spoilage of fish depends? i) The type offish. Different types of fish are susceptible to spoilage due to shape and size. A fat fish is suscept
Q. In what order should Personal Protection be removed? Personal protection is removed in following order: Gloves Mask Glasses Wash hands
Bovine spongiform encephalopathy The bovine transmissible spongiform encephalopathy (BSE), known as 'mad cow disease'-first noticed in Great Britain in 1986, is similar to scra
Write short note on glycogen storage diseases. Glycogen storage diseases are caused by genetic defects that result in deficiencies in certain enzymes of glycogen metabolism.
Give an example of each of the following types of proteins: a. Enzyme b. Structural protein c. Motor protein
Compared to the original sequence what type of mutation occurred on the following DNA sequence? Original: TACGAATTCGATAC Mutant: TACASSTTCCATAC
Define Renal Function & cardiovascular - change related with ageing? Changes associated with the cardiovascular and renal function: The progressive accumulation of athermanous
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