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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Natural Gums These are derived from various plant as well as microbial sources and are divided into the following four basic categories: 1. Seaweed gums: Agar, Alginate,
Define Types of Root Canal Perforations According to location According to location (level) in relation to crestal bone): Coronal perforation: (supragingival) coronal to
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Q. Reasons for increasing popularity of dental implants? There are various reasons for increased popularity of dental implants. With the rapid development and evolution of impl
Explain Ventricular Septal Defect in details ? Indications of surgery : Some VSDs close spontaneously or become smaller in size. This has to be taken into consideratio
Briefly discuss the Salmonella food infection. • Salmonella food infection is caused by Salmonella gastroenteritis. Transmitted by faecal contamination of foods. In
Define Nutritional Requirements of Fats and Oils during Infancy? Adequate breast-feeding ensures 30 g fat intake by infants, of which 10% is linoleic acid and 1% is linolenic a
Growth Phase -Stages in spermatogenesis Growth phase is characterized by the acquisition of the structural and functional characteristics of distinct sex cells. Also here is a
Poxvirus zoonoses Naturally occurring poxvirus infections affect humans and many species of animals and insects. However, for most part, poxviruses other than variola cause self-l
describe the phylums of invertebrates?
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