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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What do digestive enzymes do to food? Digestive enzymes suspend food; make food soluble, break large insoluble food molecules into smaller, soluble molecules
Read the information found in the Bioethics: Choices for the Future box on page 40 of the text entitled "Should you Bank your Stem Cells?" Based on the information given, discuss
RIBS- Curved or stretched 'S' shaped bones. 12 pairs, form thoracic basket, attached by head part to vertebral column and by tail part to sternum. 7 pairs a
How does aggregation occur in sickle-cell anemia? Place the steps in the correct order from no aggregation to sickle red blood cell. Note that deoxyhemoglobin is generally in the T
Q. What are the Symptoms of acute pericarditis? Chest Pain Chest pain is the most important symptom. It is retrosternal in location and patient usually locates the site o
A haem British English or heme American English is a prosthetic group which having of an iron ion contained in the middle of a huge heterocyclic organic ring called as a porphyrin.
What are plant tissues that constitute the functional structures of the leaf veins ? The Leaf veins are made of the vascular tissues.They are constituted by the xylem and the p
The ability to maintain myocardial perfusion at constant levels in the face of changing driving presence is termed autoregulation. In normal cases, autoregulation is maintained at
explane the role of nitrogenase enzyme
In humans, the recessive allele that causes a form of red-green colour blindness (c) is found on the X chromosome. a) Determine the genotypes and phenotypes of the F1 generation fr
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