Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Perception of light - floral induction, Perception of Light - Floral Induct...

Perception of Light - Floral Induction Stimulus Leaves are found to be organs that perceive light 'stimulus', K.C. Hamner and J. Bonner showed in 1938 that even if all the lea

Supplementation to prevention of iron deficiency anaemia, Define Supplement...

Define Supplementation to prevention of iron deficiency anaemia? Supplementation with low doses of iron is necessary to prevent anaemia in particular groups of people. Fortifie

Into which subphyla are the protochordates divided, Into which subphyla are...

Into which subphyla are the protochordates divided? What are some representatives of each protochordate subphylum? Urochordates (or tunicates) and cephalochordates are the two

Enzyme-controlled reaction, If an enzyme-controlled reaction normally takes...

If an enzyme-controlled reaction normally takes place at 10ºC, in general terms how will the reaction be affected by  (a) a fall in temperature to 2°C , (b) a rise in temperature t

What is the gel electrophoresis, Which of the following is a false stateme...

Which of the following is a false statement regarding gel electrophoresis? A. An electric current is used in order move DNA fragments by a semi-porous gel. B. Fragments of n

Define hepatopoietic and immune system - geriatric nutrition, Define Hepato...

Define Hepatopoietic and immune System - geriatric nutrition? Though the circulating red blood cells or the white cell count or platelet number does not normally change with a

Define central nervous system, Q. Define Central nervous system? The ne...

Q. Define Central nervous system? The nervous system begins as a simple tube during embryonic development (then anterior part expands and also ventricles are formed).  Foreb

Respiration, how respiration takes place in mammls and reptiles

how respiration takes place in mammls and reptiles

Prove thet nucleic acid is the transforming agent, Fredrick Griffith publis...

Fredrick Griffith published a series of experiments that collectively were dubbed "The Transforming Principle" in 1928. Which of the following stopped him from being able to declar

What does secondary productivity in an ecosystem indicate, a) What does sec...

a) What does secondary productivity in an ecosystem indicate? b) List any two factors by which productivity is limited in aquatic ecosystems

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd