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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain lipids? Lipids : Lipids function as energy-storing molecules such as fats and oils, protective waxes, digestive tract lubricants, heat insulation such as whale blubber
Chylomicrons, the widest of the lipoproteins, are synthesized in the intestine. They including ingested triacylglycerols to other tissues, mainly skeletal muscle and adipose tissue
Culture Medium - Techniques of Plant Tissue Culture In nature green plants are capable of synthesizing organic compounds necessary for their growth and development from the mi
Cyclosporine Immunosuppressive agent that is selective for lymphocytes mainly "T" lymphocytes. Route: Oral, parental, dose as prescribed. Side-effects: Ne
What do you understand by tegument? Outer covering of parasitic flatworms, including tapeworms and flukes, comprising a syncytial outer layer of cytoplasm connected to cell bod
Explain the Functions of Folate? Folate, also known as folic acid, is essential for good health. Folate requiring reactions include those involved in phases of amino acid metabol
In systematic four types of relationships are studied and they are: a) Relationships of phylogeny (evolutionary descent) or the degree to which two organisms are thought to be r
What is telophase? Telophase is the cell divides apart and screws itself.
Q. Where does most of the water resorbed after glomerular filtration go? What are the other substances resorbed by the nephron tubules? Only 0.5 to 1% of the glomerular filtrat
Bond specificity Some enzymes act on a particular bond (glycosidic, peptide, ester etc.). Examples: pepsin, trypsin, chymotrypsin etc. are all only acting on
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