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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Technique of food processing Freezing is another ancient technique of food processing, which allows the transportation of perishable food items for long distances from producti
Determine the term - Akinetic and Myoclonic Seizures Akinetic seizures are ordinarily seen only in children. Usually, an affected child collapses suddenly and without warning.
what is the general characteristic of aschelminths
why does the removal of extremity of coleoptile prohibit plant growth?
Define Root-End Resection Apicoectomy a. Examine the root surface before cutting for: cracks, anatomical variations, and quality of orthograde obturation. b. Cut the root en
R e striction enzyme analysis: The restriction endonuclease analysis of viral DNA fragments or DNA fingerprinting discovered in 1970s has revolutionized the study o
In which of the following functional groups ionization is stabilized by resonance? Select one: a. Carboxyl b. Hydroxyl c. Amine d. Aldehyde e. All of the above
Ask quesWhat type of antigenic agents may constitute vaccines? tion #Minimum 100 words accepted#
Q. Explain about Ecological species concept? The ecological species concept: This identifies a species as a population that occupies a distinct ecological niche, whence its rep
General process of post-hatching growth in insects While a young insect hatches out from an egg it is covered by a firm, inflexible, sclerotized cuticle, which because of its
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