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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Give some Chlorine-releasing compound? Of all the chlorine - releasing compounds, hypochlorites have been the choice disinfectants used in food industry. The hypoc
Ornithophily - Cross-pollination In tropical areas, the birds dominate over insects as important pollinators. The most common among them are humming-birds, sun-birds and honey
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Take the prepared slide of Euglena and focus it first under low power and next under the high power of tlie microscope. Observe tlie following characteristics: i) Euglena is an
Phylum Cyanobacteria Cyanobacteria are prokaryotes, but they are not like bacteria in the usual sense of the word. The cyanobacteria lack chloroplasts, and their l
Another important genus of mycotoxin producers is Fusarium, many species of which produce members of the trichothecene family of mold metabolites like deoxynivalenol, neosolaniol a
Magnesium - It is available in most of the plants, especially vegetables. By its deficiency diarrhoea is caused. Its important functions are - (a) Along with c
What is the virus that causes flu? Why doesn't the body create permanent immunity against that virus? How does the vaccine against flu work? Flu is a disease caused by the inf
In marked contrast to prokaryotic genes where proteins are encoded by a continuous sequence of triplet codons and the vast popular of protein-coding genes in eukaryotes are discont
Define Reaction for Nelson-Somogyi Method? The reagent used for enolization contains alkaline solution of cupric sulfate and Rochelle salt. Rochelle salt in the reagent prevent
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