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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
what are the different modes of nutrition
The left atrium is a thin walled cavity. Most of the wall is smooth. Musculi pectinati are present only in the auricle of the atrium. The interatrial septum separates the cavity of
What is Molting? Explain in brief. The periodic shedding of outer body covering of an animal. The term is applied to a variety of things including: loss of the outer exoskeleto
How does the excretory system of nematodes work? The metabolic residuals of nematodes are collected by two longitudinal lateral excretory channels that open in one excretory po
Q. What is Atrial Fibrillation ? As the left atrial size increase and atrial wall gets fibrosed, depolarization wave fronts get fragmented and atrial fibrillation sets in. Atri
Define Metabolic Aberrations and Clinical Manifestations? The state of obesity brings about certain alterations in the normal body processes which are enumerated herewith and h
Q. Is there an exchange of cells between the mother and the fetus through the placenta? Under usual conditions there is no passage of cells across the placenta during gestation
The pancreas produces insulin, a hormone that is specifically produced in the beta cells of the pancreas and it is responsible for the regulation of blood glucose. After carefull c
Q. Embryology in relation to taxonomy? Embryological information has been used for taxonomic purposes at various levels of classification. You know of a very basic division of
Pr o d u c t i o n Diseases The production diseases, which were previously referred as metabolic diseases, include diseases like parturient paresis (milk fever), downer
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