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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What hormone can be used to overcome jet lag? Jet lag takes place when an individual's biological clock is out of sync with local time. According to general rule it takes ab
Considering hybridization in a trait like the color of the flowers of a given plant species (red/yellow) conditioned by a pair of different alleles in relation to complete dominanc
Considering a pair of homologous chromosomes containing a gene having two different alleles how many different genotypes can the individual present? If a gene of the diploid sp
DIFFERENC E BETWEEN SPERMATOGENESIS AND OOGENESIS - S.NO . SPERM A T OGENESIS OOGENESIS 1. 2. 3.
a report on similarities and diffrences between protozoans and mesazoans
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Explain about the Glycoproteins? Most of the naturally occurring conjugated proteins are glycoproteins. Sugar molecules are covalently bound to them, especially those secreted
Actions of Gastrointestinal hormones Secretin is released under acidic conditions (low pH); digested fat or bile initiates production of pancreatic juice low in enzymes but ri
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