Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

How are ecological interactions classified, Q. How are ecological interacti...

Q. How are ecological interactions classified? Ecological interactions are classified as interspecific or intraspecific interactions and as harmonious or inharmonious interacti

Define selectivity - terminologies used in chromatography, Define Selectivi...

Define Selectivity - terminologies used in chromatography? Selectivity is a measure of preference a stationary phase shows for one solute over another and is expressed as:

What is a provirus, It is the DNA representing, the genome of virus that ha...

It is the DNA representing, the genome of virus that has been included into the DNA of the host.

Heterotrophs, Collect images of heterotrophs and make a Powerpoint Presenta...

Collect images of heterotrophs and make a Powerpoint Presentation on them

What are some factors that can lead to protein denaturizing, Protein denatu...

Protein denaturizing can be source by temperature variation, pH change, and changes in the concentration of surrounding solutes and by other processes. Most proteins are denatured

How age factors affecting the calcium absorption, How Age factors Affecting...

How Age factors Affecting the calcium absorption? Age is another factor which influences the absorption of calcium. Fractional absorption of calcium is highest in infancy i.e.,

Define points to keep in mind while feeding a preschooler, Define Points to...

Define Points to Keep in Mind while feeding a preschooler? 1. Do not force-feed the child. 2. Children naturally eat a variety of foods. Their sensory-specific satiety ensur

Golgi complex, GOLGI COMPLEX ( GOLGI APPARATUS = GOLGI BODY) The Nobel ...

GOLGI COMPLEX ( GOLGI APPARATUS = GOLGI BODY) The Nobel laureate, Camilla  Golgi (1898)  discovered  the  presence of a special,  small group  of interconnecting  membranous  s

Define diet for treatment for protein energy malnutrition, Define Diet for ...

Define Diet for Treatment for Protein Energy Malnutrition? Treatment of cases of kwashiorkor or marasmus involves mainly providing appropriate nutrition support. The child shou

Cartilaginous and the osseous tissue, Q. Which kind of tissue are the carti...

Q. Which kind of tissue are the cartilaginous and the osseous tissue? The osseous and the cartilaginous tissues are considered connective tissues since they are tissues in whic

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd