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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Question 1 Describe any two possible mechanisms of mutagenesis 2 Explain any five functions of Endoplasmic reticulum 3 Write short note on Ames test 4 Describe the structure an
biostatistical application in biotechnology
Adult (Post natal) stem cells : act as repair system for the body replenishing specialized cells but also maintain the normal turnover of regenerative organs such as blood, skin o
Define role of Zinc in controlling Gene Expression? Zinc atoms have specific structural roles in enzyme molecules, as well as, in many other proteins and in bio membranes. Thes
what is flora?
What is the tree girdling? What happens to the plant when that girdle is removed from the stem (below the branches)? Tree girdling or Malpighi's girdling is the removal from a
Key skills for healthy coping are: - Problem-solving and goal setting skills: Finding a problem and challenges, thinking of alternatives for dealing with them, testing those
wHAT IS 10um?
Succession - Community Change The factors like fire, floods and human interventions affect an ecosystem considerably. They often lead to the depletion or stripping off of orig
Inorganic Nitrogen and Sulphur Metabolism Productivity in agriculture, forestry and other ecosystems is basically limited by the availability of nitrogen nutrients like nitrat
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