Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Define phospholipids, a) Lipoproteins are large molecular compounds that a...

a) Lipoproteins are large molecular compounds that are essential to the transport of cholesterol and triglycerides (TGs) within the blood. They contain a lipid core composed of TG

#Types of Blastula, Give a detailed description of stereoblastula, please ?...

Give a detailed description of stereoblastula, please ?#

Agro industrial-evaluation of feedstuff, Evaluation of feedstuff In th...

Evaluation of feedstuff In the feed manufacturing process evaluation of the feed ingredients is very important in achieving consistent quality throughout the year. If one is a

Bioluminescence, #question.which annelids shws bioluminescence .

#question.which annelids shws bioluminescence .

Define aerobic exercise or aerobic energy system, Define Aerobic Exercise o...

Define Aerobic Exercise or Aerobic Energy System? To produce necessary energy, the body uses an aerobic pathway and an anaerobic pathway. Exercise that relies heavily on oxygen

Explain the economics in nutrition, Explain the Economics in Nutrition? ...

Explain the Economics in Nutrition? We mentioned earlier that nutritional problems affect the productivity of the individual, which, in turn affects the productivity of the nat

Cardiac resynchronization therapy, This innovative, pacemaker-based approac...

This innovative, pacemaker-based approach to the treatment of patients with heart failure who have a wide QRS complex (>140 ms) on 12-lead ECG aims at providing electromechanical c

Constituents of cell walls in bacteria, Q. What are the main respective con...

Q. What are the main respective constituents of cell walls in bacteria, protists, fungi and plants? In bacteria the cell wall is made of peptidoglycans, amongst protists algae

How to create a 20 organism ecosystem quick, i need to create a 20 organism...

i need to create a 20 organism forest ecosystem, help?

Prior to the solving of the structure of dna, Prior to the solving of the s...

Prior to the solving of the structure of DNA, Erwin Chargaff calculated the amounts of the four nucleotides (A, T,G,C) and made which of the following conclusions? A. All four

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd