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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
why is cosmozoic theory considered to be true?
Explain Procedure for Simple Staining of Bacterial Cultures? Now carry out the exercise following the steps given herewith: (1) Prepare thin bacterial smear on clean glass s
The Nurse's Role in Relieving the Child's Stres s Turning passive experiences into active ones that facilitate the child's healthy - Problems-I adjustment to hospitalization
A cat has just arrived to the veterinary clinic with low blood pressure. The cat weighs 5 kg, and as the on call Vet you want to use a bag of saline to prepare a dopamine drip for
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Q. Classify the diversity of organism in different groups • The goal of taxonomy is to develop a workable classification that reflects evolutionary relationships and provides
what is heterotroophic mode of nutrition?give some information about heterotrophic mode of nutrition?
Complete the sentence by choosing one of the given biological terms. If an ulcer continues and ultimately erodes all the way through the wall of stomach or duodenum, condition
Besides being fundamental for the activation of the main gastric digestive enzyme how does HCl also directly participate in digestion? With its corrosive effect, HCl also helps
Which of the following defines why the vast majority of segregation errors in human patients include the two sex chromosomes (XXY, XO, XYY) or chromosome 21 (triple 21)? A. Th
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