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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
green gland in prawn
Explain about the various Cooking methods? All of us eat food either raw or in cooked form. Have you ever thought why we need to cook food? Cooking is a primary process to mak
Therophytes - Classes of Life Form These are annual plants of the summer season or of the favourable season. They complete their life cycles in a single favourable season and
Restriction endonucleases are enzymes which: 1. make cuts at specific positions within the DNA molecule 2. recognize a specific nucleotide sequence for binding of DNA ligase
Define Hypoglycemic effects of Dietary Factor with antinutritional effect? Of the different carbohydrate foods tested for starch digestibility and blood glucose response, those
Define Energy needs during pregnancy Period? Two factors determine energy requirements: increase in mother's basal metabolism to support the work required for foetal growth and
are protozoans primitive to life .
A diploid cell contains three pairs of homologous chromosomes designated C1 and C2, M1 and M2, and S1 and S2; no crossing over occurs. What possible combinations of chromosomes
Q. Meaning of Gestational Diabetes Mellitus? In this type of diabetes, increased blood sugar level is first diagnosed or recognized during pregnancy. Women are not known as dia
Analyze the similarities and differences between chemosynthesis and photosynthesis. Determine what you believe is the most significant step in regard to harnessing energy within ea
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