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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
List er i o si s The disease is caused by Listeria monocytogenes - Gram positive small non spore- forming bacilli seen in soil, silage, sewage and feces of animals and b
Explain Cis-trans isomers Atoms or groups are called cis or trans to one another when they project respectively on the same or on opposite sides of a reference plane identi
H yd r o p e r i c a r d i um - h e pa titi s syndrome Also known as 'Angara disease' or 'leachy heart disease', this complex illness of poultry spread rapid
Q. Treatment of congestive cardiac failure? A judicious and careful co-ordination between oxygen support, drug therapy and nutrient intake can help in alleviating majority of t
general characteristics of phylum chordata
Compare changes in the carbon cycle to that of the nitrogen cycle on human health. Please make a conceptual graphical model for how changing the carbon cycle is likely to impact hu
Why in some cases is mitosis a synonym of reproduction? In some living beings asexual reproduction happens by many means: binary division, schizogony, budding, grafting, etc. I
What is the pineal gland? The pineal gland, also called as pineal body or epiphysis, is situated centrally in the head. It secretes the hormone melatonin, a hormone formed at n
Human lungs A pair of spongy, elastic and bag like structures of lungs is present in the chest cavity, one on either side of the heart. Lungs are enclosed by a double lay
De Vega Annuloplasty : Classic De Vega annuloplasty is done using 2'0' double armed polyester suture with pledgets on either end. It starts with one of the needles passed fr
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