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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Fomivirsen Fomivirsen, an antisense oligonu- cleotide, is FDA-approved for intravitreal treatment of CMV retinitis in HIV-infected patients who cannot tolerate or ha
Equine herpes virus infections (equine rhinopneumonitis, abortions) Equine herpes virus infections in horses and foal are caused by Alphaherpes virus of herpesviridae family p
Please I need short notes on polyphylrtic theory
Is memory loss an early sign of Alzheimer's disease? A. Memory loss is one of the earliest symptoms of Alzheimer's disease and other kinds of dementia however occasional forget
Assume that your yard has many tall trees that result in about fifty percent of the yard being shaded and fifty percent of the yard being exposed to full sun. The shaded areas and
Explain Oxygen Therapyin respiratory support? Oxygen is given to correct hypoxemia. An initial high concentration of oxygen (FiO2) is given while the underlying cause is identi
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Locomotion in Mollusca The major locomotor organ in Mollusca is the foot, which is a characteristic feature of these animals. In its simplest form the foot is a flit ventral
What is the Importance of Vitamin K The site of action of vitamin K activity is the highly complex mechanism of blood coagulation. Due to its effect on prothrombin, vitamin K i
porifera characteractericts
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