Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Define determinants of food security - food utilization, Define Determinant...

Define Determinants of Food Security - Food Utilization? It is the proper biological use of food, requiring a diet providing sufficient energy and essential nutrients, potable

Male reproductive disorders-aplasia of epidydimis, Aplasia of epidydimis ...

Aplasia of epidydimis This condition is seen in only 1 or 2 % of young bulls and is marked by absence of one (more common) or both epididymis. Bulls having a single epidydimis

Types of somatoform disorders, Types of Somatoform Disorders: Somatofo...

Types of Somatoform Disorders: Somatoform disorders are sub classified into the following categories. These include somatization disorder,  conversibtl  disorder, hypochondria

Recycling of plasma membrane components, Recycling of Plasma Membrane Compo...

Recycling of Plasma Membrane Components During exocytosis secretory vesicles fuse with plasma membrane, adding to the membrane surface area. Yet, the surface area of plasma me

Urine collection, Urine Collection: Untimed or random urine specimens ...

Urine Collection: Untimed or random urine specimens are suitable only for few chemical tests (mainly qualitative test). Usually urine sample is collected over a predetermined

Mandibles, which animal have most developed mandible

which animal have most developed mandible

Intermediate filaments - cytoskeletal structures, INTERMEDI A T E FILAME...

INTERMEDI A T E FILAMENTS These are non-living structures. Made up of different types of protein eg. Keratin, synemin, desmin and vimentin. These are divided into four

What are some evolutionary advantages of animals, What are some evolutionar...

What are some evolutionary advantages of animals with complete digestive tube? The complete digestive tube permits animals to continuously feed themselves without waiting for r

Nutritional requirement and dietary modifications in diet, Define Nutrition...

Define Nutritional Requirement and Dietary Modifications in the Diet of the Elderlu? The nutritional status influences the age-related rate of functional decline in many organ

Water and carbon dioxide towards the consumption of product, Q. What are th...

Q. What are the consequences of shifting the chemical equilibrium of the formation of bicarbonate from water and carbon dioxide towards the consumption of products of the reverse r

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd