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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is the cellular regeneration? How is the mitosis related to this process? Some tissues are capable to regenerate when injured. The liver, for instance, regenerates when
ENDOPLASMIC RETICULUM (ER) Eucaryotic celIs have two major compartments- nucleus and cytoplasm. Cytoplasm was known to have no structure until the discovery of electron micrsscop
Armamentarium 1. Edentulous stock trays 2. Rubber bowl and alginate mixing spatula, plaster mixing spatula 3. Vernier calipers 4. Alginate impression material 5. Gy
Skin The skin or integument is the outer covering of the body and have appendages i.e. nails and hairs. The outer layer of skin is known as epidermis; the inner layer is dermis
what do you understand by the term monoculture
Zone of Polarizing Activity (ZPA) The A-P axis is the 1 st axis to be fixed in the presumptive limb mesoderm at a very early stage. On the basis of results of a series of cur
What are the parathyroids? Where are they located and what are the hormones secreted by these glands? The parathyroids are four small glands embedded two in every posterior fac
Explain Identification of Cancer Cells from Normal Cells? Cancer cells can be distinguished from normal cells by examining them under a microscope. In a specific tissue cancer
Explain HIV/AIDS HIV/AIDS is another example of chronic infections. Its existence was discovered recently in 1981. The spread of HIV infection is widespread and can pro
In holly, serrated leaves are dominant to smooth-edged leaves, and red berries are dominant to green berries. Two holly plants heterozygous for leaf edge shape and berry color are
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