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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
1. The Combination Of Rapid Industrial Growth, Lack Of Regulations, Inadequate Supervision Of Safety Measures By The Authorities And Lack Of Sufficient Opportunities For The L
Q. What are organogenesis and histogenesis? Histogenesis is the procedure of tissue formation in the embryonic development. Organogenesis is the procedure of organ formation. B
Explain Point Mutations gene? Mutations involving single genes occur by addition, substitution, or deletion of a single base in DNA. Such mutations, called point mutations, ca
Q. What do you mean by Systematic Zoology ? It contains articles on animals systematic, and papers dealing with cytological attributes of species and higher taxa, distribution p
PECTIN It consists of methylated galacturonic acid, galactose & arabinose. It is also called plant cement becuase it binds layers of cell wall. It is a structural poly
Explain the Gram Staining technique? Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staini
modes of nutrition in animals?
What is biotechnology? Biotechnology is the application of biological knowledge to get new techniques, materials and compounds of pharmaceutical, medical, agrarian, industrial
Tropical seasonal forests - Ecosystem Tropical seasonal forests occur in regions where total annual rainfall is very high but segregated into pronounced wet and dry periods. T
Elaborates the Pathophysiology of ventricular septal defect? The shunt in the VSD is left to right. The magnitude of the shunt is determined by the size of the VSD and the, lev
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