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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Regulatory enzymes Regulatory enzymes: Citrate synthase, isocitrate dehydrogenase and a-ketoglutarate dehydrogenase complex are the key enzymes which regulate
Q. Defination of Behaviour Change Communication? Behaviour Change Communication (BCC) is an interactive process with communities to develop specific messages and methods using
What is the Use of Uristix There are some enzymatic products and reagents impregnated on paper or plastic strips and dipping them in urine provide the results in less time comp
What is Subphylum Crustacea and Subphylum Uniramia? The Arthropoda are usually grouped into four subgroupings called subphyla (singular-subphylum). One of these four subphyla -
Transfer RNA molecules are notable for having generally nucleotides shown in the figure such as 1-methylguanosine (m1G), pseudouridine (Ψ), dihydrouridine (D), inosine (I) and
Unconventional Feed Resources and Anti-Nutritional Factors With increasing ruminant population, there is a need to identify and introduce new and lesser-known food and feed cro
Q. What is the tympanum? In which part of the ear is it located and what is its function? The tympanum (or ear drum) is a membrane located in the middle ear just after the audi
Q. List five advantages of DSME? Following are the advantages of DSME. It enables the patient to 1. Accept the disease. 2. Gain knowledge about disease, its prevention
Determine Protein requirements of school children and adolescents? FAO/WHO/UIW (1985) recommends factorial method to compute their protein requirements. The factorial value is
Which type of cell came first in evolution - the eukaryotic cell or the prokaryotic cell? This is an interesting problem of biological evolution. The most accepted hypothesis a
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