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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Surface area The rate of oxidation increases in direct proportion to the surface area of the lipid exposed to air. Furthermore, as surface - volume ratio is increased; a given
Eutrophication - Effect on Water Bodies Moderate quantities of sewage decay naturally in a water body and water gets purified after some time. But the problem arises when the
What are brief descriptions of how you could test hypothesis. or how would you know if your hypothesis is testable?
Q. What is the difference between diffusion and osmosis? Osmosis is the phenomenon of movement of solvent particles in general, water from a region of lower solute concentratio
Necessity of Transport in Phloem Leaves produce photoassimilates and support various tissues including roots. The excess photoassimilates and metabolites stored in specialised
Q. What are immunoglobulins? Immunoglobulin is the alternate name given to antibody and Immunoglobulins are complex proteins containing a variable portion and an invariable por
P a r a typhoid and other Salmonella infections Paratyphoid salmonellae are non-host-specific. The commonly reported species are S. Typhimurium, S . Enteritidis S . T
how do you define sex
What is the typical shape of poriferans? Sponges have bodies in the type of tubular vases or globes open in the upper extremity. They have an internal central cavity and porous
Somatic Embryogenesis The process of embryo development is called embryogenesis. It is not the monopoly of the egg to form an embryo. Any cell of the female gametophyte (Embr
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