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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
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steps of digestive system in amoeba,with a clear picture description
Transposable elements are able to move around the genome by precise or imprecise excision events. What is meant by imprecise excision? A. Imprecise excision refers to a transpo
Q. What is the relation between the hypophysis and the thyroid? The hypophysis secretes TSH (thyroid- stimulating hormone). This hormone hastens the secretion of thyroid hormon
Based on your reading of the article "The Inner Life of the Genome" which of the following is a wrong statement regarding the organization of chromosomes within the nucleus? A.
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An adequate level of pressure is needed in the arteries to keep the blood flowing through the cardiovascular system. The chief determinant of arterial pressure is the volume of blo
Explain the Disadvantages of tissue punch - There is a loss of keratini-ed tissue and this can lead to a compromised soft tissue barrier in cases where there is deficiency of a
Q. What is an example of a lab experiment that shows the variation of the photosynthesis effectiveness in relation to different photic energy frequencies to which the reaction is e
Temperate deciduous forest - Ecosystem The temperate forests are characterised by a moderate climate and broad-leafed deciduous trees, which shed their leaves in fall, are bar
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