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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
pls explan 2 puc lesson
Q. What is the water cycle? The water cycle represents the recycling and circulation of water in nature. The Liquid water on the planet surface is heated by the sun and turns i
Natural Gums These are derived from various plant as well as microbial sources and are divided into the following four basic categories: 1. Seaweed gums: Agar, Alginate,
(a) Hormones of the Anterior lobe - The anteriorIobe of the pituitary gland secretes the following hormones, most of them are trophic hormones. (i ) Somatotropic hormone o
Valvular Heart Diseases Normal heart valves function to maintain a uni-directional flow of blood through cardiac chambers. Two basic problems that compromise the normal functi
Define Functions of fluoride? The only beneficial role demonstrated for fluoride is in reducing the prevalence and severity of dental caries in children and adults. This is en
Urea is known to denature proteins Based on the structure of urea, how do you think this occurs?
As the name shows, the cytoskeleton is responsible for the supporting of the normal shape of the cell; it also acts as a facilitator for substance transport across the cell and for
Gene expression is controlled by chromatin state, _______ and _________. a. transcription factors and epigenetic regulators b. polymerases and control proteins c. delta and tau pro
Q. What is Etiopathology? Who is likely to develop gout? What are the risk factors? Let us find out. Gout is caused when there is over production of uric acid in normal purine
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