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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are plant hormones? Plant hormones, also known as phytohormones, are substances that control the embryonic development and the growth of the adult plant.
Besides mercury which other heavy metals cause toxic pollution? Examples of other heavy metals that cause toxic pollution are lead, cadmium and chromium.
Q. Dietary Management fo oesophagitis? Providing adequate nutrition support may require emphasis of different aspects during acute and chronic oesophagitis. In acute phase,
A charged particle A exerts a force of 2.39uN to the right on charged particle B when the particles are 12.3 mm apart. Particle B moves straight away from A to make the distance be
how does epigenetic controls transcription?
Insulin was the first protein to be sequenced biochemically. Assuming that there were no introns involved in the process, what are the possible DNA sequences that produced the last
What is the difference between ulcerative colitis and Crohn's disease? The differences between the two include: I) Anatomic distribution of the inflammatory process: Crohn'
Pituitary The pituitary is also known as hypophysis. It is composed of two embryologically distinct tissue. The anterior pituitary or adenohypophysis is derived from the roof
Q. What is the structural depiction of a carboxyl group? Carboxyl groups have a carbon attached to one hydroxyl group by a simple bond and one oxygen by a double bond. The othe
Define Requirements of Chromium? There is no Recommended Dietary Allowance (RDA) for chromium but adequate intakes that can be used as a goal for individual intakes has been pr
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