Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Explain the lactose fermenters and non lactose fermenters, Explain the Lact...

Explain the Lactose Fermenters and Non lactose Fermenters 1) Lactose Fermenters - These produce acid on lactose fermentation, which results in red colouration on bacterial colo

Phylum , General characteristics of phylum

General characteristics of phylum

What is enzyme assay, What is Enzyme assay Enzyme assay may also be use...

What is Enzyme assay Enzyme assay may also be used to determine whether stored plant products are suitable for use as food commodities, for e.g.  α-amylase should be present

Explain the use and care of microscope, Explain the Use and Care of Microsc...

Explain the Use and Care of Microscope? Proper care and maintenance of microscope is needed. Following points should be kept in mind while handling the microscope: (i) Instr

Regulation of blood glucose level, REGULATION OF BLOOD GLUCOSE LEVEL Va...

REGULATION OF BLOOD GLUCOSE LEVEL Various levels of regulation are exerted at substrate level, hormonal level, enzymatic level and at organ level on carbohydrate  metabolism  s

A common biocontrol agent for the control of plant diseases, A common bioco...

A common biocontrol agent for the control of plant diseases is : 1. Baculovirus 2. Bacillus thuringiensis 3. Glomus 4. Trichoderma Trichoderma

Purposes of assessing growth and development in children, PURPOSES OF ASSES...

PURPOSES OF ASSESSING GROWTH AND DEVELOPMENT IN CHILDREN Before you assess the growth and development you should be aware of the purpose of monitoring. The purposes are a

Dietary management during congestive cardiac failure, Q. Dietary Management...

Q. Dietary Management during congestive cardiac failure? Nutritional care is a little difficult in congestive cardiac failure. This is because oedema complicates the nutritiona

How campylobacteriosis is caused, How Campylobacteriosis is caused It i...

How Campylobacteriosis is caused It is caused by  Campylobacter jejuni, a Gram-negative rod. It is a microaerophillic organism, which means it has a requirement for reduced lev

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd