Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Signify the term - pectoral girdle., Signify the term - Pectoral girdle. ...

Signify the term - Pectoral girdle. Bones in vertebrates that connect the appendages on the left and right side of the anterior appendicular skeleton to each other. Pectoral gi

Dietary tips for hyperlipideinic patients, Q. Dietary tips for hyperlipidei...

Q. Dietary tips for hyperlipideinic patients? It is important to note here that the dietary modifications need to be individual in each patient and these should be tailored lo

What are the main types of waste, Q. What are the main types of waste? ...

Q. What are the main types of waste? The waste can be classified into many kinds, each of them carrying its own different environmental problem: recyclable waste, organic waste

Requirements of glutamine in diverticular disease, Q. Requirements of Gluta...

Q. Requirements of Glutamine in diverticular disease? Glutamine: Wile specific nutrients that may have an impact on diverticula disease have not been studied as thoroughly as t

Mechanism of regeneration, MECHANISM OF REGENERATION - T.H. morgan reco...

MECHANISM OF REGENERATION - T.H. morgan recognised 2 primary mechanisms - M o r ph o l axis Epim orphosis 1. Production of enti

Telophase of karyokinesis, - The chromosomes at each pole uncoil and elonga...

- The chromosomes at each pole uncoil and elongate to form the chromatin. - A nucleolus reappears at each pole. - Spindle fibers and asters disappear and centrioles split. - A nu

How do bones grow, Q. How do bones grow? Bones form and develop through...

Q. How do bones grow? Bones form and develop through a process known as ossification. There are two types of ossification: endochondral ossification and intramembranous ossific

Chamaephytes - classes of life form, Chamaephytes - Classes of Life Form ...

Chamaephytes - Classes of Life Form The perennating buds and organs are borne on shoots close to but just above the ground. The buds receive protection from fallen leaves and

Explain enfuvirtide, Explain Enfuvirtide Enfuvirtide is the first fusio...

Explain Enfuvirtide Enfuvirtide is the first fusion inhibitor approved by the FDA for treatment of HIV infection and is indicated for treatment-experienced patients with ongoin

Why are lysosomes known as the cleaners of the cell waste, Q. Why are lysos...

Q. Why are lysosomes known as "the cleaners" of the cell waste? Lysosomes carry out heterophagic and autophagic digestion autophagic digestion by digesting residual substances

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd