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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
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what is gaseous exchange
Does pH affect the enzyme activity? The concentration of hydrogen ions in solution affects the enzyme activity. Every enzyme has maximal efficiency under an optimum pH. As p
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How does the presence of thick, cloudy, yellowish fluid in the peritoneal cavity differ from the normal state?
Complications : IABP can compromise blood flow to the leg at the time of insertion, pumping or after removal of balloon. It may also cause perforation, bleeding, thrombosis, embol
Explain Polio Adults who have not previously been immunized against polio should receive a primary series of inactivated polio vaccine (IPV) if traveling to areas where polio i
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