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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Adverse Effects of Valacyclovir Valacyclovir is generally well tolerated; adverse effects are similar to those with acyclovir. GI disturbance, headache, rash, CNS effects such
A biologist examines a series of cells and counts 140 cells in interphase, 10 cells in metaphase, 4 cells in anaphase and 7 cells in telophase. if complete cell cycle requires 24 h
Define Anaerobic Exercise or Energy System? Thus, exercises which are done for short bursts of time lasting only for few seconds (less than 120 sec) use only anaerobic system o
Q. Explain about Oral Glucose Tolerance Test? This is most commonly used diagnostic test particularly for identifying new and ‘at risk' individua1s.Thi.s test is carried out af
Please help with the following: 32P is an unstable isotope of phosphorus that has a half-life of 14.29 days. Atoms of this isotope undergo beta decay in which a neutron decays i
Explain Water soluble vitamins for Infants? Thiamin, riboflavin and niacin requirements are met through breast milk alone and solely breast-fed infants meet their requirements.
what is life process
1. Given a log phase bacterial culture with 1 x 106 cells per ml and a generation time of 20 minutes, how long does it take the culture to reach a density of 6.4 ? 107 cells per ml
Use of micro-organisms to control insect pests, pathogens or weeds constitutes biological control, and the biological agents employed are called biocontrol agents. Micro-organis
Give an example of how a functional group can affect the elements of an organic compound. The hydroxyl group on alcohols is polar and this produces alcohols polar compounds.
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