Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Who is heterozygous at 6 loci, Given an individual who is heterozygous at 6...

Given an individual who is heterozygous at 6 loci, how many different gametic Genotypes are possible? A. 36 B. 12 C. 32 D. 48 E. 64

Life processes, mode of nutrition : out side the body

mode of nutrition : out side the body

How far from the building should the fire hose be located, A fire hose ejec...

A fire hose ejects a stream of water at an angle of 38.8 ° above the horizontal. The water leaves the nozzle with a speed of 26.6 m/s. Assuming that the water behaves like a projec

Radioisotopes, Radioisotopes Radioisotopes are those forms of an elemen...

Radioisotopes Radioisotopes are those forms of an element which exhibit rzdioactivity and so can be used as markers or tracers. For this purpose, we first introduce the radiois

Advantages and disadvantages in usage of solid fuels, Advantages (1)   ...

Advantages (1)   Liquid and gaseous fuels can be manufactured from solid fuels. (2)   Relatively cheap and easily available. (3)   These are easy to transport. (4)   A

Determine the occurrence of vitamin e, Determine the Occurrence of Vitamin ...

Determine the Occurrence of Vitamin E The tocopherols are widely found in animal and vegetable materials, like nuts and seeds. Considerable amounts are found in a number of

Supplementation to prevention of iron deficiency anaemia, Define Supplement...

Define Supplementation to prevention of iron deficiency anaemia? Supplementation with low doses of iron is necessary to prevent anaemia in particular groups of people. Fortifie

Adaptations, Adaptations Every organism can live and reproduce within a...

Adaptations Every organism can live and reproduce within a certain range of climatic conditions. Organisms that live in hot or cold environments have behavioural and physiologi

Female reproductive system - vagina, V AGIN A - Median, elastic, musc...

V AGIN A - Median, elastic, muscular tube 7.5 cm long. Open into vestibule by vaginal orffice. The lining forms vaginal rugae. Space between vaginal wall & cervix is forn

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd