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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Factors Influencing RS Content of Foods? Different fractions of RS mentioned in Table may be naturally present in some foods or may be generated as a result of industria
Determine the term - Neuropsychological approach The neuropsychological approach relies on the use of tests in which poor performance may indicate either focal (localised) or d
Borna disease Borna disease is fatal neurological disease of horses and sheep caused by a virus (RNA) of the family Bornaviridae and the disease is characterized by sub acute meni
describe food procurement and digestion in ciliates.
On a given microscope, the numerical aperture of the objective is 1.5. The microscope is provided with a filter that selects a wavelength of 500 nm. What is the limit of resolution
Endodontic success - According to Strindberg 1956 the endodontic success is the postoperative clinical as well as radiographic satisfaction after a predetermined postoperative
What is Septal Ablation (TASH/PTSMA) ? This is a nonsurgical interventional treatment. The septal myocardium supplied by 1 st septal branch of left anterior descending artery
CHF complicating IE is primarily the result of valve destruction or distortion or rupture of chordae tendinae. Intracardiac fistulas, myocarditis, or coronary artery embolization m
Have you ever said, ‘I cannot cope up with this?' Or have you heard any one who is sick in your family saying, ‘I cannot cope with my sickness' or ‘I can not take care of this sick
Oogenesis Oogenesis is the formation of ovum from oogonial cells that are formed in the ovary from primordial germ cells. And as in spermatogenesis it involves meiosis to pro
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