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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Which structures of the body have more bones for their size than any other part of the body? Hand and wrist have more bones in them for their size than any other part of bod
what are the methods of seed formation
The secretary coil fills the lumen with a NaCl solution that is isotonic with the blood plasma. As this solution moves upward through the reabsorptive duct, NaCl is reabsorbed into
Phase Contrast Microscopy Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in liv
COMPOSITION Proteins = 44-76%, Lipids = 20-53%, Carbohydrates = 1-8%, (Protein-Lipid ratio = 0.8 : 1 to 4 : 1) Most of lipid are "Phospholipids" which are amphipat
What is Gum ghatti Gum ghatti, also known as Indian gum, is amorphous, translucent exudates of the Anogeissus latifolia tree of the Combretaceae family. Gum Ghatti is composed
Nuclear run-on is a technique used to estimate the relative rate of the transcription of a given gene, as opposed to steady-state level of the mRNA transcript (which is influenced
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what is exonephric
one idea on how plant physiology can apply in food engineering
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