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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q In which environments bacteria live? Bacteria can be found in a variety of environments throughout the planet. There are bacteria in the air, on the surface, in fresh water,
Explain the Sub-clinical Protein Energy Malnutrition? You have already learnt that clinical forms of PEM represent only a small proportion of the total cases of PEM in a commun
What is mitosis
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What grade did you get in biology?
Q. Explain Type 2 Diabetes Mellitus? It is the most common form of diabetes accounting for 90-95% of patients with diabetes. Type 2 Diabetes Mellitus was previously called as N
Consider an axon of a neuron. At time=t1, its voltage is at threshold for an action potential; at time=t2, its voltage is at 0 millivolts prior to the peak of that action potential
Can enhancers work in Trans? That is, can an enhancer on one piece of DNA activate a promoter on another piece of DNA? Propose an experiment to test this. Be sure to discuss any ap
Classify bones structurally and embryologically(developmentally). Structural classification of bone: Macroscopically can be classified into two types, compact and trabecul
What is heterospermy?also give its significance?
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