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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define tuberculosis Tuberculosis (TB) is still a problem in the United States, even though the incidence continues to decline in most of the country. Treatment of TB can be div
Explain carbohydrates requirement during thyphoid Carbohydrates: Carbohydrate intake should be liberal. This can be attributed to: 1. Repletion of glycogen stores. 2.
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what is the difination of osmosis
Explain the term of measurement of height in veous pressure? Measurement of Height: Measurement of height of venous pressure is done from the sternal angle of Louis verticall
Define Regents for Estimation of Reducing Sugar by Fehling Soxhlet Method? Fehling A solution - copper sulphate solution Fehling B solution - alkaline tartarate so
#qprokroyotic flagella structure and protocoluestion..
Dietitians and nutritionists Dietitians and nutritionists, who are associated with hospitals and clinics generally have regular work hours. At times, they may be required to wo
A person notices a mild shock if the current along a path through the thumb and index finger exceeds 84 µA. Compare the maximum possible voltage without shock across the thumb and
Vegetative Development - Growth It is quantitative and irreversible change. It reflects an increase in size and volume of an organism. For example, the change in the size of a
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