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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How do contraceptive pills generally work? Contraceptive pills generally have the hormones estrogen and progesterone. If taken daily from the 4th day after menses the abnormal
It is immune difficult mediated hypersensitivity. Typical manifestations contain rheumatoid arthritis, serum sickness, necrotizing etc.
Relate Mitochondria organelles with eukaryotic cells Mitochondria are highly specialised organelles in eukaryotic cells. These perform the energy transducing process, and are o
Q. What are respectively some remarkable functions of albumin, myosin, CD 4 , keratin, immunoglobulin, reverse transcriptase, hemoglobin and insulin? Myosin is a protein that w
Drawbacks of Lamellar model This model does not explain the elasticity of PM. Although this model says that PM is elastic but it also states that head of lipid molecules is
Pulmonary edema is life-threatening condition and therefore, treated as a medical emergency. As is the case with chronic stable heart failure, identification and correction of any
Q. Can Nutrition affecting the growth of microorganism? Ans. Food is food to humans and microbes - be it carbohydrates, proteins or fats. The nutritional requirements of b
Q. Chronic exposure of Cadmium? An increase in the soil cadmium content due to soil pollution by industrial wastes, burning of coal, fossil fuels, sewage sludge, medical and
Explain the Properties Locust bean gum Locust bean gum is slightly soluble in room temperature water and must be heated to 75 to 85 o C for complete hydration and viscosity dev
Peste des petits ruminants (PPR) is an acute, highly contagious viral disease of goats and sheep caused by peste des petits ruminants (PPR) virus which belongs to the genus Morbil
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