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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Following ways can be used for control of noise pollution: It can be controlled by designing, fabricating and using quiter machines. In heavy industries, sound proof insul
Adverse Effects of Bronchospasm Nasal and throat discomfort can occur. Bronchospasm, sometimes severe, has been reported uncommonly in patients with reactive airway disease; za
Q. How does the pancreatic juice resume the digestion of carbohydrates? What is the involved enzyme? Carbohydrate digestion begins with the action of the salivary amylase (ptya
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a) How is the milk production regulated by hormones in human female? Define. b) Explain the importance of the auxin / cytokinin ratio in plant tissue culture.
Neurological and Sensory System There are three neurological systems: sensory, motor and autonomic. Sensory system consists of sensory receptors, sensory nerve fibres. Differen
Q. Explain about Glycemic Index? Although the use of exchange lists is still popular for planning diabetic diets, it has been realized in recent years that in exchange lists th
Define Changing Body Composition and Techniques for Measuring Body Composition? Changing body composition has been suggested as a pathway to explain age-related functional decl
Define Swollen and Tender Joints and haemorrhage in various Tissues? Haemorrhages occur deep in muscle, particularly in calf, thigh, buttocks and forearm, causing pain in surro
Disability evaluations after injuries: (a) Explain the following terms: "duration of disability" and "degree of disability". (b) Explain how degrees of disability after injur
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