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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Sterol Regulatory Element Binding Proteins (SREBPS)? These belong to a general family of transcription factors. They are synthesized as membrane embedded proteins in res
Name the methods for extracting phosphorous Phosphorus/phosphate - Phosphorous is estimated as phosphate. Some methods for extracting phosphorous are as follows: Dyer met
Which of the following is not needed for C to U deamination during RNA editing? A. Formation of localized segments of double stranded RNA. B. The 5' and 3' efficiency elemen
Q. What is rigor mortis? Dead bodies are at first limp. Many hours after death, skeletal muscles undergo a partial contraction that fixes the joints. This condition, called rig
Erysipeloid A zoonosis called erysipelas in animals, rose disease in pig, erysipaloid in man is caused by Erysipelothrix rhusiopathiae (insidiosa). Other names given to this co
How is lymph propelled through the lymphatics? Some of the larger lymphatics are capable to contract, or else the lymph is propelled by body muscles which contract and 'squash'
Explain the Prevalence of iron deficiency anaemia? We can find out about the prevalence of anaemia if we know what percentage of population is suffering from anaemia. The WHO h
Congenital disorders Poor fertilizing quality of semen could be due to impaired spermatogenesis. When such a condition is due to genetic congenital causes the treatment is not
Define some adverse effects of saponins? The adverse effects of saponins may be described as under: - A well-known toxic effect is 'erythrocyte lysis' since they interact wi
Selective Destruction - Wildlife The selective destruction of one species of an existing fauna can produce equally unfortunate results. The perfect demonstration of unexpected
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