Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Deficiency diseases-copper deficiency, Deficiency of vitamin -A (hypovitami...

Deficiency of vitamin -A (hypovitaminosis-A) Vitamin A deficiency develops due to insufficient supply of the vitamin or its defective absorption from alimentary tract. The defi

While meiosis results in four diploid cells, Which of the following best co...

Which of the following best compares the results of mitosis and meiosis? Mitosis results in four diploid cells, while meiosis results in two haploid cells. Mitosis results in four

What is invertebrates, What is Invertebrates? Invertebrates: About 99...

What is Invertebrates? Invertebrates: About 99% of all the animals lack backbones, and are invertebrates! Invertebrates include the phylum Arthropoda, or the animals with joi

How many bones in the human face, Explain how many bones in the human face?...

Explain how many bones in the human face? Ans) 14 bones are present in human face and these bones are known as sepio

Cardiovascular, Describe the process of excitation-contraction coupling (EC...

Describe the process of excitation-contraction coupling (ECC) in the heart. How do adrenaline, noradrenaline and acetylcholine alter heart rate and or force?

Explain secretion of parathormone and calcium blood level, What is the rela...

What is the relation between secretion of parathormone and the calcium blood level? The parathormone enhances the calcium blood level as it stimulates the resorption (remodelat

Explain inhibitors, Explain Inhibitors Inhibitors: Citrate synthase i...

Explain Inhibitors Inhibitors: Citrate synthase is inhibited by ATP, NADH, succinyl CoA and acyl CoA derivative of fatty acids (fatty acyl CoA). The rate of the reaction  is

Chloroplasts , Chloroplasts Like mitochondria, chloroplasts are also di...

Chloroplasts Like mitochondria, chloroplasts are also distinctive organelles of eucaryotic cell. But they are found only in plant cells. Chloroplasts contain membranes forming

State the symptoms of epilepsy, State the symptoms of epilepsy Three p...

State the symptoms of epilepsy Three particular symptoms are found in many types of epilepsy: 1) An aura, or warning, of impending seizure: This aura may take the form of a

Define about the sporocyst larva, Define about the Sporocyst Larva? Sp...

Define about the Sporocyst Larva? Sporocyst is the second larval stage in the life cycle of F. hepatica. It develops from the miracidium larva within the pulmonary chamber of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd