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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are niches and competitive exclusion?
The allele that causes albinism (p) is recessive to the allele for normal pigmentation (P). A normal woman whose father is an albino marries an albino man whose parents are both no
Explain the Interaction of Vitamin A with Vitamin E? Of the various nutrients, the interaction of vitamin E, proteins, zinc and iron with vitamin A is of significance. Know? Le
Embryology This is the study of growth, fertilization, division or differential of the zygote into embryo of early development of living beings before the attainment of structure
Explain theTechnique of total anomalous Surgery ? Corrective surgery is undertaken as an emergency procedure after diagnosis has been confirmed by two-dimensional echocardiogra
Q. What is mitral stenosis? Most common cause of mitral stenosis is rheumatic fever. Nearly 30 per cent of patients with rheumatic fever may go on to develop pure mitral valve
#what is the habitat of phylum protozoa
The bases in DNA have carbon-nitrogen ring structures; due to the nitrogen atoms they are known as nitrogenous bases. There are two parts of ring structure. Adenine and guanine are
Q What is the kind of nitrogen waste that mammals eliminate? Like chondrichtian fishes and adult amphibians, mammals are ureotelic that is they excrete urea. Q. How do plac
Development of Nutritional Biochemistry Nutrition, in general, and particularly nutritional biochemistry, has traveled a long way since 1926, when nutrition was first reco
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