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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Drug effects on Lipid Metabolism? Drugs can affect the metabolism of various essential nutrients in the body. These impairments are highlighted herewith: Lipid metabo
a nutrient agar plate labelled 10^(-5)ml had 154 colonies after incubated. what is the cell density in the original sample
How nutritional status influences the age? The nutritional status influences the age-related rate of functional decline in many organ systems in the body. Nutrition is also an
Types of dietary adaptations for trerapieutic needs Normal nutrition is the foundation upon which therapeutic modifications are based. We have already discussed in previous
How do sponges try to protect themselves against harm from the environment? Is that method efficient or rudimentary? Sponges can close their pores to avoid the entrance of wate
Regeneration in Hydra You previously know that the Hydra has spectacular regenerative ability. The hydra is a small tubular, two layered fresh water animal computing 20mm in
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Explain about the DNA viruses - Carcinogenic? The three DNA viruses found to cause human cancers are EBV, HBV and HPV. What are these? Let us find out. Epstein-Barr viru
Quantitative Methods - Therapeutic diets Quantitative Methods: These are often essential for constructing Lherapeutic diets. The two ways by which this could be done are as
Hypoglycaemia Unawareness ? Some people with diabetes do not have early warning signs of low blood glucose, a condition called hypoglycemia unawareness. ? This conditio
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