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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Left internal mammary artery (LIMA): It is most often used for bypassing left anterior descending (LAD) coronary artery and its diagonal branch. The right internal mammary
Explain the Ebb or Shock Period - Dietary Management for Burns? During the initial bums after injury, the focus is on counteracting the stress induced neurohormonal and physiol
Q. What are the two major divisions of the chordate phylum? Chordates are divided into vertebrates and protochordates (urochordates and cephalochordates). Q. What are the d
Q. What are the venous of the valves system? What is their function? The valves of the venous system are structures inside the veins that permit blood to flow only in the norma
Briefly explain the difference between stabilizing selection, directional selection, and disruptive selection.
Explain the Chemical Properties of Monosaccharides? As you already know the chemical properties of monosaccharides depend on the presence of the hydroxyl the aldehyde or the ke
Advantage of solar energy: 1. It is renewable: it produce comes from sun, so it is plentiful and will always be there as long we need it . 2. Solar
Fatty acid breakdown happens in the cytosol of prokaryotes in plants in peroxisomes and in the mitochondrial matrix of all other eukaryotes. By entering the mitochondrial matrix,
Young's Trichromatic Theory According to Young's theory, three types of cones exist, each sensitive to a particular pigment-rythrolabe (red), chlorolabe (green), and cyanolabe
What is the functionality of Lungs in human biology? The right lung consists of three lobes, and the left lung, two lobes. Both lungs hold between 5 to 6 L of gas, called the t
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