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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Portal Hypertension: If you review the portal circulation you may recall that normal blood flow to and from the liver depends on proper functioning of the portal vein, the he
Q. Show overall management of diabetes? Diabetes as we all know is a disease with ever increasing prevalence in' our country. It can be described as a disease characterized by
Why is glucose such a good source of energy for bacteria? 1) Glucose contains six oxygen atoms, which can be used for oxidative phosphorylation and the generation of many ATP molec
Q. What is an instance of a hypothesis which may explicate why there is not a big representation of the class Reptilia found in Polar Regions? Beings of the class Reptilia are
Q. What is the functional unity of the kidneys? The functional (filtering) unity of the kidneys is the nephron a nephron is made of efferent arteriole, afferent arteriole, glom
Nuclease is an enzyme which degrades the nucleic acids. A nuclease can be DNA-specific (a DNase), RNA-specific (RNase) or non-specific. It might act only on single stranded nuclei
what three connective tissues are liquid
Grouping of Stem Cells: The range of differentiation “Potency” is an important property of Stem cells & serves as the basis for classifying cells as : Totipotent SCs -capa
Q. Classification and Justification of hemichordata? Kingdom Animalia Animals; multicellular organisms with cells that lack a cell wall, many capable of inovement or movement o
Is there vaccine against tuberculosis? The vaccine against tuberculosis is known as BCG (bacillus Calmette-Guérin). BCG is not used in some countries where tuberculosis is not
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