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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Isomerization of citrate Isomerization of citrate: In this step, citrate is isomerized to isocitrate by aconitase which has iron-sulphur centre as its prosthetic grou
Healthy Person X is walking on level ground. Which of the following is true for the knee extensor muscle of X's right leg during the step cycle? A. The right knee extensor mus
It is mostly spread by sexual contact, blood transfers and from HIV infected mother to child.
Isomerization of dihydroxyacetone phosphate Isomerization of dihydroxyacetone phosphate: Triosephosphate isomerase interconverts dihydroxyacetone phosphate and glyceralde
Regarding solubility, how are lipids classified? Fats and oils are hydrophobic molecules, i.e., they are non polar and insoluble in water. Lipids in general are molecules with
what are phototrophs
Define isolated soybean proteins in Seafood products? The most important application in this category is the use of ISP in fish sausage and surimi based restructured fish produ
It is a neural mechanism that plays a role in encoding of new information. Encoding takes place as a result of changes in the strength of synapses among neurons in neuronal network
The patient will appear anxious and dyspnoeic. Patients in chronic heart failure are usually malnourished and even cachectic. Chronic passive venous congestion may produce exophtha
give a detail account of modes of locomotion in protozoa
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