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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Infectious and Chronic Diseases The most prevalent diseases of poverty, many of which are also infectious by nature, are malaria, tuberculosis, respiratory infections, water b
What is the Mode of Nutrition in mosquito?
EXCRETIO N IN FLATWORM - Main organs are flame cells, having bundle of cillia. It receives solute and water from surrounding tissue fluid by ultra filtration & discharge th
assignment on biodiversity management
Explain the Importance of nicotinic acid Nicotinic acid is successfully used in the therapy of a number of vascular disorders such as vascular spasms, angina pectoris, arthriti
Define the Type of chemical reaction used in volumetric analysis? The titration has to be based on some rapid chemical reaction. Although any type of chemical reactions may be
Q. Value of biodiversity? Today, there is widespread concern for conservation of wild animals and plants. One might wonder why there is so much concern about protecting other s
Spermatogenesis: In the mature male functional sperm cells are produced within the seminiferous tubules of the testes. Around the periphery of the seminiferous tubules are l
Review of Characteristics of Phylum Nematode and Its Position in Animal Classification? Nematodes may be free-living or parasites of plants or animals. however, all nematode
ECOLOGY
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