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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Homologous Recombination is the exchange of the sequence between two associated but different DNA (or RNA) molecules, with the result that a new "chimeric" molecule is obtained. S
How we write assimgment about mycobiology of mycoplasma in detail
On living organism Air pollution causes following effects on living organism: (a) Effects on humans: 1. Carbon monoxide reduces the oxygen carrying cap
Another possible way to classify organisms would be to separate them into unicellular and multicellular organisms. Explain why this is not a useful classification system. Other
Dominance In each community, a few over topping species are present in greater bulk. By their greater number or biomass (living weight) the dominant species modify the habitat
Disability evaluations after injuries: (a) Explain the following terms: "duration of disability" and "degree of disability". (b) Explain how degrees of disability after injur
1. Biogas is produced by the activity of aerobic bacteria on animal waste 2. Methanobacterium is an aerobic bacterium found in rumen of cattle 3. Biogas, commonly called goba
STRUCTUR E OF A MYOFIBRIL - The dark bands of the myofibril are termed the A-bands (Anisotropic bands). Each A-band has at its middle a light zone called H-zone (Henson'
Q. Treatment and Management of Diarrhoea? You must have realized by now that diarrhoea should not be neglected and must receive prompt medical care to minimize the frequency of
(1) It should have high "calorific value" or heat content. (2) It should have "moderate ignition temperature". (lgnition temperature is the minimum temperature at which it s
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