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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Yellow fever Yellow fever also known as Black Vomit and American plaque is a viral disease primarily of monkeys and transmitted to man by female Aedes mosquitoes. It is caused
Explain Free Water - Water Found in Food? Water which is bound by such minute forces, that its vapour pressure is equal to the vapour pressure of pure water. It can be found as
COMMON RESPIRATORY DISORDERS: Respiration is one of the most vital functions of the body. The purpose of respiration is to provide oxygen ta the body cells and to remove exc
Explain Light in cell Metabolism? Energy for almost all organisms on earth is derived directly or indirectly from light energy produced by the sun. Sunlight consists of energy
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Explain the Acoelomates - Animals without a Body Cavity? The simplest group of animals that has bilateral symmetry and a solid body (acoelomate) is the Platyhelminthes. Phy
Define the effect of Vitamins (A, D, K and B-Complex) on athletes? Vitamins A, D and K have been found to have no ergogenic effects. Ingesting large doses of these vitamins hav
Q. What is Supraventricular Arrhythmias? Such arrhythmia tend to be reduced by the vagal withdrawal accompanying the onset of exercise.This and wandering pacemakers tend to rec
Q. What are the Keys for Identifying plants and animals? Keys are determined as devices to help in identifying plants and animals. These are merely an aid to identification bas
What technique would you use to see if two proteins interact with each other inside of a cell?
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