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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
what are disadvantage of protozoa
Which of the following statements about buffers is true? Select one: a. A buffer composed of a weak acid of pKa = 5 is stronger at pH 4 than at pH 6. b. At pH values lower
Determine the term - Language Abilities The study of aphasia and acquired language disorders was one of the driving forces in the growth of neuropsychology in this century. Th
Explain Understanding the response of farmers? Understanding the response of the farmers by the government also influences the food production. Government policy influences the
Question 1: Write the applications of Oracle clinical in Pharmaceutical industries Explain what is "Oracle clinical" Explain different application of oracle clinical
File & chemical removal of gutta percha: - K-type file & chloroform is the best choice in small and curved canals. - The pulp chamber floated with chloroform, then k-typ
Making individual rock collections Peoples should be encouraged to make their own collection of rocks. Small pasteboard or cigar boxes will serve to be the collections. The spe
conductometry and its uses in biological experiments
What is Passive Transport? Passive Transport : Specialized proteins called permeases also move specific substances across the cell membrane in a process called carrier transp
In order to be able to study the neural basis of perceptual and cognitive functions, it is necessary to have some knowledge of brain anatomy (i.e., what the names of different brai
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