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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
phyla under protozoans
Why does geographical isolation lead to speciation? The Geographical isolation between groups of the same species leads to formation of a new species since it disallows crossin
Q. What do you mean by Oesophagitis? We already know that oesophagus is a muscular tube 25 cm in length and basically helps in transporting the food from the mouth to stomach,
What are the parts of Nervous system Nervous system is divided into three main parts: 1) The brain and spinal cord or Central Nervous System (CNS). 2) The nerves or Peri
What is biology
Q. Filming Projection and Technique? Biplane ventriculography is preferred over single plane ventriculography because it gives more information without additional risk to the p
Cyanotic Congenital Heart Disease So far we have discussed the acyanotic congenital heart disease. Now we shall discuss some of the main cyanotic congenital heart diseases.
Rheumatic Fever and Rheumatic Heart Diseases Rheumatic fever is an inflammatory disease of the heart potentially involving all layers of the heart. The resulting damage to t
Define Titrimetric Method - 2, 6 Dichlorophenol Indophenol Method? The titrimetric method is a redox titration method which depends on the reduction of the blue dye 2,6 dichlor
ENZYME INHIBITION Enzymes are often inhibited by the presence of suitable inhibitors. Much of current drug therapy is based on this. Basically, there are three major cla
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