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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Family is the Basic Unit of Society Each child within a family needs love and security to develop feelings of trust and self esteem. Each child is a unique individual who h
Q. Describe Bentham and Hookers System? Bernard de Jussieu (1699-1776) tried to classify the plants in Royal Garden, Paris. During this exercise he developed a system of classi
Q. What is the molecule that donates hydrogen for photosynthesis, in sulfur photosynthetic bacteria? In sulfur photosynthetic bacteria the substance that donates hydrogen is hy
Digestion The nutritional requirements and the various ways used by heterotrophic organisms to obtain nutrition. Whether food is used to give energy or to build the body, the
What is Asexual Reproduction? Asexual reproduction occurs in prokaryotes through the process of binary fission. Primitive eukaryotes and plants also reproduce by various t
Hans spemann theory
What is Intermittent ST Depression ? Ans. Several patients progress from variable ST-segment depression, often associated with respiration, to the classic ST-segment chang
Q. Can you briefly explain about parasitism? The Parasitism is an inharmonious interspecific ecological interaction in which individuals of a species (the parasites) explore or
Physical map is the map of locations of the identifiable landmarks on DNA (for example restriction of the enzyme cutting sites, genes), regardless of their inheritance. Distance i
What are sols? A colloidal system in which solid particles are dispersed in a liquid is referred to as a sol, to distinguish it from a true solution. In a true solution, the
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