Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Fatty Acids, How can I construct a fat molecule whose fatty acid tails are ...

How can I construct a fat molecule whose fatty acid tails are 4 carbon atoms long

Explain the working of specific acquired immunity, Working of Specific Acqu...

Working of Specific Acquired Immunity Primary lymphoid organs, as you already know, are thymus and bone marrow. Secondary organs are lymph nodes, spleen and lymphoid tissue. Wh

Ecosystem degradation and wildlife, Ecosystem Degradation and Wildlife ...

Ecosystem Degradation and Wildlife Man is an animal with ecological requirements just like any other animal. It constantly needs a source of energy and.minera1 nutrients for i

Determine the steps in surgical therapy, Determine the steps in surgical th...

Determine the steps in surgical therapy a) The first step in surgical therapy is to reflect the tissue and degranulate the defect. b) It is followed by exposing and treating

Define the vital force hypothesis, How did Pasteur's experiment answer the ...

How did Pasteur's experiment answer the objections raised by supporters of the "vital force" hypothesis? Pasteur's experiment allowed air from the out- side to mix with air fro

Define the term - stimulus, Define the term - Stimulus Stimulus and res...

Define the term - Stimulus Stimulus and response characteristics of the tests themselves, as well as of the test instructions, become exceedingly important considerations. In g

Classification of living organisms – species, Classification of Living Orga...

Classification of Living Organisms – Species You have by now already come across the word 'species'. The word has a definite meaning in biology. Although the living world is v

Explain heat preservation - method of food preservation, Explain Heat Prese...

Explain Heat Preservation - Method of Food Preservation? The process of heating was used centuries before its action was understood. Food is heated up or cooked. Heat kills mi

Relation between the hypophysis and the hypothalamus, Q. What is the relati...

Q. What is the relation between the hypophysis and the hypothalamus? The hypothalamus is a part of the brain situated just above the hypophysis and the hypothalamus gets centra

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd