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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Fats requirement in diabetes? Fats: The total fat recommended by WHO is less than 30% of the total calories. However in view of the widely prevalent Asian paradox in India,
Q. Spastic and Obstructive constipation? Spastic constipation: It results from excessive tone of the colonic muscle. Obstructive constipation: It occurs usually due to obstr
Production of ATP is an endothermic reaction. How much energy in Kcal is having in 1 molecule of ATP?
Characteristic features of Phylum Cnidaria All are aquatic animals. Radial or biradial symmetry around an oro-aboral axis, but no head. Diploblastic, with an epidermi
What are the Properties of titanium Titanium has several favourable properties like low specific gravity with a density of 4.5 gm/cm 3 , high heat resistance and high strength
Explain about the Shade Drying? Shade drying is carried out for products which can lose their colour and/or turn brown if put in direct sunlight. Products that have naturally v
#q1. If offspring exhibit a 3:1 phenotypic ratio, what are the genotypes of a parent?uestion..
What are some examples of human cells that produce proteins for exportation? Which cytoplasmic organelle is expected to be well-developed and abundant in those cells? Specializ
classification of plan''s tissue
Local Needs, Choices and Circumstances Beyond obvious choice for private sector, the best public-private balance for a country depends on its local culture and circumstances.
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