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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Describe the concept of soil testing Different grades of complex and mixed fertilizers are being marketed to suit the soil and crops of a region. The recommendations are made o
Explain Environmental Sampling - Methods and Techniques? Environmental sampling can be done for total microbial load or for some specific pathogens or spoilage organisms. The m
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Which of the below describes the type of fruit characterized as a dry, simple, one-seeded indehiscent (pron: in-deh-HISS-ent) fruit with seed attached to one ovary wall at only one
Q. Areas affected in peptic ulcer? The areas affected in peptic ulcer (due to erosion) can be: 1. Lower part of the oesophagus. 2, stomach (lesser curvature - an urn, whe
nutrition in euglena
Which of the following mediates the interactions among the splicing machinery and the mRNA transcript? A. Alpha helical structures within the RNA binding domain of the splicing
how nerve signals are transmitted
What is visual accommodation? Visual accommodation is the phenomenon of varying the curvature of the crystalline lens to make possible the variation of its refractivity to adju
FUNCTION S - (a) It is antagonistic to glucagon. Insulin decreases the level of glucose in the blood. It acts by increasing the rate at which glucose is transported out of the
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