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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Plant classification according to Ancient Greeks and Romans? Hippocrates, "The Father of Medicine" (460-377 B.C.) is reputed to have been one of Democritus 's disciples. He
What is Community Ecology explain their characterstics? Community Ecology: In ecological terms, a community consists of an assemblage of all of the populations living and int
Pulses Careful examination of both upper and lower limb pulses is useful in detecting coarctation, and other arterial stenosis. The carotid arteries should be checked for sten
What is the classification of this phylum
what is the skeleton in the different classes of coelentrata known
The organisms that live in hostile environments that cannot support other forms of life are members of what kingdom?
Define Functions of Inorganic Phosphorus? The major functions of inorganic phosphorus include: a) Structural component of bones and teeth: Phosphorus is a part of calcium ph
Embryonic stem cells: can differentiate into all cell types of the specialized embryonic tissues when given sufficient and necessary stimulation for a specific cell type, e.g. Plu
Toxoplasma gondii Another well known Sporozoan is Toxoplasma gondii which has an unusually wide host- range for n protozoan parasite. It can probably infect all warm-blooded a
What is Ascending Aortic Aneurysm ? Ascending Aortic Aneurysm : In aneurysms confined to ascending aorta well below the innominate artery origin, the technique used is
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