Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Consistently overwatering a potted tomato plant, Consistently overwatering ...

Consistently overwatering a potted tomato plant will eventually kill it. Using the map, suggest why waterlogged soil results in plant death. O2 cannot reach respiring root cells. T

Define both drosophila and bacteriophage, Alfred Sturtevant and Seymour Ben...

Alfred Sturtevant and Seymour Benzer's studies of recombination in both Drosophila and bacteriophage demonstrated which of the following? A. The gene is an indivisible unit and

Explain the objectives of congenital heart disease, Explain the Objectives ...

Explain the Objectives of Congenital Heart Disease ? After reading this unit, you should be able to: 1 Comprehenced the epidemiologic significance of congenital heart disease (

Structure of human spermatozoon, Structure of human spermatozoon: Na...

Structure of human spermatozoon: Nature: Human sperms are minute, microscopic and motile. Structure: Each sperm have an oval shaped head piece, a neck, a middle piece and

What is meant by unsaturation or saturation of oils and fats, Q. What is me...

Q. What is meant by unsaturation or saturation of oils and fats? When it is said that a triglyceride is saturated it means that in its molecule the carbon chain is bound in its

What are the alleles of a gene, What are the alleles of a gene? The Dip...

What are the alleles of a gene? The Diploid individuals have paired chromosomes. For instance in humans there are 23 pairs of chromosomes totaling 46 chromosomes. Every pair co

Body fluids of marine teleost, Body Fluids of Marine Teleost The body ...

Body Fluids of Marine Teleost The body fluids of marine teleost are hypotonic to seawater, so these fishes lose water to the environment especially across the gill epithelium.

Biota of pelagic zone, Biota of Pelagic Zone Pelagic region constitute...

Biota of Pelagic Zone Pelagic region constitutes 90 per cent of the total ocean surface and is less rich in species and numbers of organisms than the two regions discussed bef

Rules of nomenclature classification, Q. Rules of Nomenclature classificati...

Q. Rules of Nomenclature classification? The Rules of Nomenclature prescribed the categories into which plants should be classified. These categories constitute the units of cl

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd