Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What is diagnostic wax up, Diagnostic Wax Up A diagnostic wax up should...

Diagnostic Wax Up A diagnostic wax up should be done by you or got done from a laboratory for each and every case regardless of the extent or difficulty level of the case as it

Advantages of fertilizers and manures, Advantages of Fertilizers and manure...

Advantages of Fertilizers and manures Fertilizers and manures are necessary for maintaining the soil in a high state of fertility and productivity.  It is important to know the

How to grow root hairs, How to grow root hairs Hairs can simply be seen...

How to grow root hairs Hairs can simply be seen on the roots of mustard seed grown on a damp flannel. Seeds placed on an earthenware dish standing in a soup plate having water

Defects of heart, DEFECT S OF HEART 1 .      Blue Baby syndrome (Cya...

DEFECT S OF HEART 1 .      Blue Baby syndrome (Cyanosis) - Due to persisting foramen ovalis in atrial septum even after birth, the impure blood from right auricles comes to

What is micrographia?, Micrographia is abnormally small, cramped handwritin...

Micrographia is abnormally small, cramped handwriting and/or the progression to continually smaller handwriting. This is one of the symptoms of Parkinson''s disease.

Diabetes mellitus in the world, Diabetes Mellitus in the World As per t...

Diabetes Mellitus in the World As per the International Diabetes Federation Directory, in 1994 the global burden of the disease was estimated at 110 million. These cases will i

Why in the f1 generation is one of the colors missing, Considering hybridiz...

Considering hybridization in a trait like the color of the flowers of a given plant species (red/yellow) conditioned by a pair of different alleles in relation to complete dominanc

Why is it advantageous to have weak hydrogen bonds, Why is it advantageous ...

Why is it advantageous to have weak hydrogen bonds among complementary bases and strong covalent bonds among phosphate and Deoxyribose groups in a DNA molecule? The hydrogen bo

Dreams, what do our brain and heart do when we are asleep?

what do our brain and heart do when we are asleep?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd