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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the Glass Test Tubes - Food Microbiology Sterile glass tubes are used for culturing microorganisms in liquid or solid media. Sterility in tubes is maintained by using c
What is cell theory? Cell theory declares that the cell is the constituent unit of living beings. Before the discovery of the cell, it was not identified that living beings
Functional regions of digestive system In general the digestive system of metazoans is divided into 4 major functional regions of: Reception Conduction and s
Define the meaning of value - Soil Value refers to relative lightness or darkness of a colour and is a measure of the amount of light that reaches the eye under standard lighti
Origin of Cell Wall - The cell wall is product of cytoplasm. The cytoplasmic organelles such as endoplasmic reticulum, golgi apparatus play a very important role in the f
bird without syrinx
Define hexose monophosphate pathway The hexose monophosphate pathway (HMP also called the pentose phosphate pathway, or phoshogluconate pathway) consists of Mo irreversible o
name of the excretory organ of a lizard
Define Limitations for underwater weighing method? This method is expensive, time consuming and usually requires a lot of equipment and space. The subject needs to be
ELECTRON MICROSCOPY (EM) In EM, the object is viewed by a beam of electrons instead of visible light. An electron beam has a shorter wadelength (h = 0.004 nm) compared with vis
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