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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. which of structures is the nuclear membrane composed? Eukaryotic cells have nucleus delimited with two juxtaposed membranes that continue with the membrane of the endoplasmi
Do disinfectants kill bacteria? Secure various types of commercial and house- hold disinfectants. Inoculate as more culture dishes as you have samples of disinfectant and singl
Hormone s . Cortical steroids (corticoids-hormones of adrenal cortex) are grouped into three catagories : mineralocorticoids, glucocorticoids and gonadocorticoids. (i) Mine
phylum porifera
Emulsions You may have eaten Mayonnaise. Some of you may also know how it is made. Mayonnaise is a liquid in liquid dispersion, in fact a true emulsion. A true emulsion re
Deciduous Senescence - Senescence Only the leaves senesce as in many trees. The senescence of leaves, or abscission occurs when at the base of a leaf a layer of cells is laid
Extraradicular Infections Whatever the cause of post endodontic disease we should do proper diagnosis to determine what is the cause; is it intra or extraradicular infection ,
Define Source of Electrons - Growth of Microorganism? Depending upon the source of electrons used by microorganisms, these are known as - a) Lithotrophs (rock eaters) - use
he Lac Repressor protein, which we will talk about a bit later in the course, is important for controlling expression of a number of genes in E. coli. There are about 10 copies of
Glycogenesis : This is an anabolic process in which glucose is polymerized into glycogen by the sequence of reactions. Since glycogenesis occur in all body cells .but
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