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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
An incubation combination is set up having the single-stranded DNA template, DNA polymerase I, the primer and all 4 deoxyribonucleoside triphosphates (dTTP, dGTP, dCTP, dATP),
Functions of the Vascular System The major function of the circulatory system is to provide a transport mechanism for the blood flow to and from the heart. The vascular syste
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Explain how a cell produces and releases proteins. Proteins are made on ribosomes and packaged into vesicles by the Golgi apparatus. The vesicles move to the cell membrane and
general characteristics of sponges
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Define Treatment and Management of Adverse Food Reactions? Management of adverse food reactions involves diagnosing the problem followed by nutritional and medical care.
Q. Is it expected that a change in the primary, in the secondary or in the tertiary structure of a protein will produce furthermore functional consequences? Any change of the p
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Ensuring Food Safety It is now well-known that the end product inspection and testing does not provide safety of food. It has inherent limitation of mapping the potential ha
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