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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
what is osmoregulation.
Functions of the Vascular System The major function of the circulatory system is to provide a transport mechanism for the blood flow to and from the heart. The vascular syste
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Metazoan Branches The Kingdom Animalia comprises all the multicellular animals (metazoans). These are divided into three branches: Mesozoa Parazoa Eumetazoa
main branches of biology
Although there is much talk in the news about stem cell research, the public and policymakers need to understand how basic body cells work to transmit information and replicate to
Q. Describe the metabolism of lipids? Fats are split into fatty acids and glycerol by the enzyme. They are absorbed in small intestine and recombined into fats. It is stored in
Do echinoderms present internal or external fecundation? Is there sex division among individuals? The fecundation in echinoderms is external; gametes are liberated in water whe
Q. Dietary management of dyspepsia? Keeping in mind the etiology, symptoms and complications of dyspepsia it must be clear that treatment and management of [his disorder does n
Foot as Burrowing Organ The Scaphopoda that usually have burrowing habit, possess foot suitably modified for burrowing purpose. The conical and protrusible foot of Dentalium
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