Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

How is litmus paper, How is litmus paper and testing for acid, base, and ne...

How is litmus paper and testing for acid, base, and neutral, related to biology?

Why on earth would be drinking milk to feel better, If a person has a stoma...

If a person has a stomach ulcer (that is aggravated by excessive hydrochloric acid {HCl} secretion ), why on earth would he or she be drinking milk to feel better, when milk is als

What is extruded starch, Extruded Starch  Cereal products, that are pr...

Extruded Starch  Cereal products, that are prepared by pumping cereals through a small opening for instance, pasta, Ready-to-eat cereals etc.

Explain the virtual dissection of the rat, Explain the Virtual Dissection o...

Explain the Virtual Dissection of the Rat in multimedia tutorials? This animation will teach you about basic mammalian anatomy. The major organ systems in a rat are very simila

Evolution and growth of sports nutrition as a discipline, Define Evolution ...

Define Evolution and Growth of Sports Nutrition as a Discipline? An academic discipline is basically a formal body of knowledge discovered, developed, and disseminated through

Zoonotic diseases and trade, Zoonotic diseases and trade Disease and t...

Zoonotic diseases and trade Disease and trade have a long-standing, interwoven history. During the 14th century, Europeans realized the value of exotic nature of goods and mat

Explain the classification of hydrocolloids by function, Classification of ...

Classification of hydrocolloids by function It must be clear to you now that gum ghatti, gum karaya, gum tragacanth ( classified as exudate gums) locust bean  gum, guar g

Collection of cerebro-spinal fluid (csf), Collection of Cerebro-spinal Flui...

Collection of Cerebro-spinal Fluid (CSF) Examination of the CSF is one of the most useful laboratory procedures in neurological diagnosis. Four types of punctures ma

Carbon dioxide for photosynthesis, Aim: To prove that Carbon dioxide is ess...

Aim: To prove that Carbon dioxide is essential for photosynthesis. Apparatus: A potted healthy plant with long and narrow leaves, a wide mouthed glass bottle, split cork. Chemicals

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd