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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Chromosomes Karl Nageli and Hofmeister observed chromosomes in pollen mother cells of Tradescantia. Chromosome name proposed by Waldeyer. During cell division,
Define Decreased oxygen availability at high altitude (Hypobaric hypoxia)? The governing biophysical factor at high altitude is decrease in barometric pressure with increase in
Types of Surgery : Pulmonary valve replacement using pulmonary or aortic allograft is the procedure of choice. It increases R.V. ejection fraction, decreases R.V. end diastolic p
Hypothalamus functions are generally aimed at maintaining A region in the brain beneath the thalamus; consists of number of aggregations of nerve cells and controls a variety of a
Q. What is an instance of a hypothesis which may explicate why there is not a big representation of the class Reptilia found in Polar Regions? Beings of the class Reptilia are
Q. What are the major structures of chloroplasts? Chloroplasts are involved by two membrane layers the inner and the outer membranes. Inside the organelle the formative unit is
i have question that all scientist agree that the basic unit off life is a cell and scientist said this to be a cell theory... and they also said that if a theory survive continuou
Examine the differences between DNA and RNA. Explain why DNA is the most favorable molecule for genetic material and how RNA compares to it in this respect.
Are the following statements true for eukaryotes , prokaryotes, both groups, or neither group? Answer They use a deoxyribonucleoside triphosphate as their energy currency"D They us
How are the epithelial tissues classified? The epithelial tissues are classified according to the shape of the cells that form it (epithelial cells may be cuboidal, columnar, o
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