Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Invertebrates - osmotic and ionic regulation, Invertebrates - Osmotic and I...

Invertebrates - Osmotic and Ionic Regulation Regulation of water and ions in invertebrates is done by neuroendocrine mechanism. It operates at the level of Malpighian tubules

Explain pro-oxidants - lipid oxidation, Explain Pro-Oxidants Transitio...

Explain Pro-Oxidants Transition metals, particularly those possessing two or more valency states and a suitable oxidation - reduction potential between them (e.g., cobalt, cop

Preference share capital or quasi-equity, Preference Share Capital or Quasi...

Preference Share Capital or Quasi-Equity It is also called quasi-equity because it combines features of equity and those of debt.  It is preference because it is preferred to

Treatment of diabetic ketoacidosis, Q. Treatment of diabetic ketoacidosis? ...

Q. Treatment of diabetic ketoacidosis? The components of treatment of NKHDC are: 1) control of water loss. 2) control of sugar using insulin. 3) adjust electrolytes li

Photosynthesis, how do i prove in a diagram that water is needed for photos...

how do i prove in a diagram that water is needed for photosynthesis

Difference between self pollination and cross pollination, What is the diff...

What is the difference between self pollination and cross pollination? Which of these two modes of pollination contributes more to the plant diversity? Self pollination happens

Drugs and insulin used in diabetes, Q. Drugs and Insulin used in diabetes? ...

Q. Drugs and Insulin used in diabetes? When diet, exercise or even weight reduction do not improve the diabetic symptoms and blood sugar levels, the uses of hypoglycemia drugs

Determine the principle of neuropsychological assessment, Determine the Pri...

Determine the Principle of neuropsychological assessment The neuropsychological assessment of school age of children and adolescents is not primarily a technological enterpris

External events contribute to gain of heat in the body, What (a) internal, ...

What (a) internal, (b) external events contribute to gain of heat in the body?   a) Respiration in the tissues, particularly in the brain and active muscles, is the major in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd