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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Are the limbs modified into wings of bats and the wings of birds examples of evolutionary analogy or homology? What about whale fins compared to fish fins? Bat and bird wings h
Q. What are the fundamental constituents of the cell membrane? The cell membrane is formed of lipids, carbohydrates and proteins. The membrane lipids are phospholipids, a sp
Explain Voriconazole It has a spectrum of activity similar to itraconazole but appears to be more active against Aspergillus spp. And some species of Candida, containing C.
Interferon alfa In about one third of adults and children with chronic hepatitis B, treatment with interferon alfa-2b leads to loss of HBeAg, return to normal aminotransferase
Q. What is the chorioallantois membrane present in the embryonic development of birds and reptiles? How does this membrane participate in the energetic metabolism of the embryo?
Why do ribosomes move along mRNA during translation? During translation the ribosome always exposes two mRNA codons to be translated by moving along the mRNA. When a peptide bo
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Q. Fats requirement in pectic ulcer? Fats: These delay the gastric emptying. The products of fat digestion in the small intestine stimulate entrogastrone, which inhibits gastri
why obwlia is considered to be of special interest in zoologyas an animal showing an intermediate grade of organisation
On what structural level of the enzyme (primary, secondary, tertiary or quaternary) does the enzyme-substrate interaction depend? The substrate binds to the enzyme in the activ
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