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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is Organelles? Organelles : Eukaryotic cells contain various membrane-bound structures called organelles, in contrast to prokaryotes, which lack a definite internal or
what is a crabs excretory system?
Maintain nutrition Your responsibility as a nurse is to take care of nutrition and prevent the child from starvation. Breast feeding'should be continued during the diarrhoea e
Define Absorption, Storage and Elimination of cyanocobalamin? Vitamin B 12 in food is bound to proteins and is only released by the action of a high 'Concentration of hydroch
Based on your reading of the article entitled "The Evolution of Color Vision", which of the following is a false statement? A. Trichomatic vision is found in all male and femal
All the cells present in the body contain genes and an approach targeted to modulate the expression of genes is known as gene therapy. This novel approach has emerged by the end of
Q. What is the lasting form in the gametophyte, pteridophytes or the sporophyte? How can it be compared to bryophytes? The lasting form in pteridophytes is the diploid (2n) spo
Explain the Disadvantages of Colonies Obtained At Different Dilution 1. Heat sensitive microorganisms may be damaged by melted agar, giving low viable count as compared to spre
What is polymorphism
Biomaterial is defined, in the broader sense, as any pharmacologically inert material that is capable of interacting with a living organism without causing adverse reactions either
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