Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Define excretion of iron in human body, Define Excretion of Iron in Human B...

Define Excretion of Iron in Human Body? Our body has a limited capacity to excrete iron once it has been absorbed. Daily losses in adult man are between 0.9 to 1.05 mg. About 0

Determine the patellar reflex, How is it explained that a person with the s...

How is it explained that a person with the spinal cord sectioned at the cervical level is still able to perform the patellar reflex? The arch reflex depends only on the integr

Classification of diabetes mellitus, Classification of Diabetes Mellitus ...

Classification of Diabetes Mellitus a) Type1Diabetes Mellitus i) Type 1 a ii) Type 1 b b) Type 2 Diabetes Mellitus c) Others i) Gestational Diabetes Mellitus

Timber, introduction,types,uses and characteristics of a good timber?

introduction,types,uses and characteristics of a good timber?

Onion cells, What is the process of mitosis in onion root tips in a lab

What is the process of mitosis in onion root tips in a lab

What is the function other than protection of the ribs, What is the functio...

What is the function, other than protection, of the ribs? The ribs help to alter the volume of the thorax during breathing movements.

Terms of incubation time and the generation time, If the generation time (t...

If the generation time (t) is the incubation time (t) per generation (G), or t = t/G, rewrite the formula you derived in question 2 for bacterial population growth in terms of incu

What is the binding between two amino acids called, What is the binding bet...

What is the binding between two amino acids called? The chemical bond among two amino acids is called a peptide bond. Protein Structure Review - Image Diversity: peptide bo

What is class crinoidea, What is Class Crinoidea? This Class includes t...

What is Class Crinoidea? This Class includes the sea lilies and the feather stars. What makes them different from the other Echinoderms is the placement of their mouth and anus

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd