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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Upon admission the DPU nurse notes: Fasting status - fasted from 12 midnight, no morning medication taken. Consent - signed Pre anaesthetic check Test results
uses and advantages and disadvantages of immunolocalization
discuss why obelia is considered to be a special organism in zoology
Zymogen :- an inactive form of an enzyme; becomes active prior to its action.
What is Neuroscience? Theoretical, mathematical, and computational approaches to queries in neuroscience are now recognized as valid by experimental physiologists. The main neu
Autoradiography is the process to detect radioactively labeled molecules (which commonly have been separated in an SDS-PAGE or agarose gel) based on their ability to develop an im
Determine the Syndrome of Listeriosis Syndrome: Listeriosis in humans is not characterized by a unique set of symptoms since the course of the disease depends upon the state
Would you expect the muscle fibers of the tongue to be striated or smooth? What about the muscle of the diaphragm/ Explain your answer.
Q. What is Tall T-Wave? It has been reported that tall T-wave during or after exercise indicate inferior wall ischaemia. However, Ellestad reports the findings that tall T-wav
Energy Loss - Energy Flow Let us now take the second point that is the loss of some energy at each trophic level. You might recall that the second law of thermodynamics states
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