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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain about the Adequate Intake (AI)? If sufficient data are not available to establish an EAR and hence RDA, the AI is derived instead. The AI is derived from observations o
Reproduction Living things do not arise spontaneously. They arise only from pre-existing living things This is one of the fundamental tenets of biology. The ability of an orga
Explain Changes in feeding behaviour of infants? On maturation of neuro-muscular system, the body is able to coordinate sucking, swallowing and breathing. Till about three mont
Foscarnet (Foscavir) Foscarnet is an alternative to ganciclovir and valganciclovir for treatment of CMV infection. It is approved for use in CMV retinitis, including progress
The Polymerase Chain Reaction Kary Mullis established the technique of the polymerase chain reaction (PCR) in the year of 1983. He won a Nobel Prize for the procedure in the ye
Define Absorption, Storage and Elimination of vitamins? Reading through this section you would realize that all fat-soluble vitamins undergo similar metabolic fate. They are a
What is the meaning of Prohaptor? The specialized attachment sucker which surrounds the mouth in monogenean flukes. It comrpise adhesive structures which help attach the animal
Dermato phytoses Dermatophytosis (ringworm) is a cosmopolitan, superficial and highly contagious disease of man, animals and birds. The infection is caused by fungi of three g
Q. Non - Direct Approach to counseling? The counsellor's participation is minimal; here the counsellee freely expresses. The counsellor pays attention to the emotion and attitu
What is the ranges of tolerance and performance optima? Ranges of Tolerance and Performance Optima : When it comes to physical factors or resources, most species are able to
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