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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Environment and Health- Impact of Air and Water Pollution Environment includes a host of factors of which air and water are the two major components. Air pollution is caused b
Since hypoglycaemia can occur in any diabetic patient at any time, it is important for you to learn the causes of hypoglycaemia. Such knowledge will help you to educate the patient
Tube or Enteral Feeding Ideally the patient must be fed orally, but in cases where the patient is unable to take solid foods, a part or all of intake is usually given by the tu
What are the parts of a surgical needle. How do you classify surgical needles The surgical needle is comprised of 3 parts: the needle point, the needle body, and the swaged (pr
The excretory organs and excretory materials of plants and animals
Explain about Blood pressure Blood pressure is explained as the force exerted by the blood against the walls of the arteries. Blood pressure (BP) can be described as the fo
Q. What is the function of the antidiuretic hormone? Where it made and which is the stimuli that increase or reduce its secretion? The antidiuretic hormone is secreted by the h
Notes on branches of embryology
MOD E OF HORMONE ACTION THROUGH EXTRACELLULAR RECEPTORS - The molecules of amino acid derivatives, peptides or polypeptide (protein) hormones bind to specific receptor molecul
When conducting a hair comparison, what factors would you consider?
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