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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Cellular level - Level of body organization The next higher level of organization is the cellular level. This is really an aggregation of cells that are functionally different
Explain the Systemic Metabolic Responses? Many of the metabolic responses to infection are similar to those following injury. The key changes include: Hyper metabolism: Oxyg
Explain Food Sources of lron? lron is found in foods in one of the two forms i.e. haem or non-haem. In the human diet, the primary sources of haem iron are the haemoglobin and
NE R VE IMPULSE (IT'S GENERATION) - It is an electro chemical phenomenon manifested through ionic movement across cell membrane. It was discovered by A. L. Hodykin & A
write a note on mode of nutrition
Q What are the biological troubles that molluscs face due to their soft body? Because molluscs have a soft body they are further fragile. They also have more complexity to supp
How can the concept of recombination frequency be used in genetic mapping? Genetic mapping is the determination of the location of the genes in a chromosome. By determining
feeding in holozoic organisms
Why is quarrantine a must before introduction of a plant species from a dissimilar country? a) What is meant by apoplast pathway? Why does it occur in cortex and not in endoder
Adverse effects of rifamycin No severe adverse effects were observed in clinical trials with rifaximin. There have been a few postmarketingeports of hypersensitivity reactions
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