Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

How are mammals characterized, Q. Mammal identity card. How are mammals cha...

Q. Mammal identity card. How are mammals characterized according to examples of representing beings, skin, respiration, nitrogen waste, basic morphology, circulation, thermal contr

protozoa, explain protozoa taxonom

explain protozoa taxonomy

Adrenaline and noradrenaline, Both these neurotransmitter strengthen memory...

Both these neurotransmitter strengthen memory when they are released into the blood stream following learning. Stressful events stimulate release of stress hormones from the adrena

What do you understand by pericardium, Q. What do you understand by Pericar...

Q. What do you understand by Pericardium? Pericardium is the covering sac of the heart. The pericardium prevents acute cardiac chamber dilatation. Acute pericarditis, pericardi

What is the phase of the menstrual cycle, Q. In general what is the phase o...

Q. In general what is the phase of the menstrual cycle when copulation may lead to fecundation? Although this is not a rule, to be effective fecundation in general must occur w

Predisposing factors and pathphysiology of meningitis, Predisposing Factors...

Predisposing Factors The patients with diabets mellitus, malignancies and those on immunosuppressive drugs have reduced resistance and are more susceptible to develop meningi

Choice of graft material depends on, Choice of graft material depends on ...

Choice of graft material depends on - Its nature towards bone e.g., Osteoconductive or osteoinductive - Its architecture i.e., granular or block, pore si-e - Its origin -

Explain phylum sarcdina, Phylum Sarcdina 1) They move by means of pseud...

Phylum Sarcdina 1) They move by means of pseudopodia (ralse feet) or similar structures. 2) They feed heterotrophically by phagocytosis.   Some examples: Amoeba, Entamo

Carbohydrates requirement in chronic diarrhoea, Q. Carbohydrates requiremen...

Q. Carbohydrates requirement in chronic diarrhoea? Adequate amount of carbohydrates i. e 60-65% of the total energy should be given to the patient. Easily assimilated carbohydr

Importance of research in nursing, Importance of Research in Nursing: ...

Importance of Research in Nursing: It is  the responsibility of the nursing profession  to discover, verify, structure and restructure the professional knowledge through  syst

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd