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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How did Pasteur's experiment answer the objections raised by supporters of the "vital force" hypothesis? Pasteur's experiment allowed air from the out- side to mix with air fro
Agricultural activities - Cause of Air Pollution Agricultural activities too are a major cause of air pollution. About 60 to 65% of carbon dioxide is produced from burning of
what are the modes of nutrition in different animals
Explain Evidence of coverage of Ecology Evidence of coverage of Ecology and Evolution to the level of Descriptor 2 and an evaluation or discussion that shows depth of underst
define alimentary canal
Please answer the following question on Sequence Y: Protein databases 1. What sequence from which organism is this sequence most similar to? 2. Can you find any homo
Functionality of implant material It should take maximum advantage of available bone and permit the maximum amount of forces to be transmitted through the implant within physio
Benthic Zone - Organisation of the Marine Ecosystem The benthic zone is divisible into sub zones horizontally. These are depicted in a cross section portion of the marine habi
Classification of Enzymes It has been a long tradition of giving enzymes names ending in 'ase'. The only major exception to this 'ase' is the proteolytic enzymes, whose names
Q. Periapical radiography - criteria for endosteal implants? Periapical radiographs are images of a limited region of the mandibular or maxillary alveolus. Periapical radiogra
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