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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Explain Spoilage by fungi and bacteria? The dominant spoilage organisms in fruits are fungi, as their pH is low (normally 5),whereas the pH of vegetables is fiom 5.0 to 7
What is Infra-diaphragmatic Type of TAPVC? Initial stages of operation are the same as for supracardiac type of TAPVC. The heart is lifted out of the pericardial cavity and c
what is the advantage of being dark skinned?
Which of the following is true for the ATP-sensitive potassium channel? A. The channel is a spanning protein with a receptor site for ATP located on an extracellular region of
Nutritional care can be recognized as both a science and art. Can you tell why? It is considered as a science because the raped advances in scientific knowledge provides all h
Q. Show the parameters which are of interest in CHD? Total Cholesterol: Serum totnl cholesterol equals the sum of HDL-cholesterol (HDL-c), VLDL-cholesterol (VLDL-c) and LDL-cho
Q. How to Investigate mitral stenosis by Electrocardiogram? Electrocardiographic changes are not specific for mitral stenosis and are never diagnostic for any valvular heart di
Q. Considerations for congestive cardiac failure? - Subjects with congestive cardiac failure often tolerate small frequent meals better than larger infrequent meals as these a
Metalimnion - Summer Stratification This zone lies below the epilimnion and above the hypolimnion and thus forms the intermediate layer which is non-circulating. The metalimni
Q. Describe about coronary reactive hyperemia? Myocardium depends almost completely on aerobic metabolism. Occlusion of a coronary artery even briefly produces an increase in c
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