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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Yersinia enterocolitica The genus Yersinia contains of 3 human pathogenic species, Y. enterocolitica, Y. pseudotuberculosis and Y. pestis, among which, Y. enterocolitica finds
Explain the life cycle of Water? The water cycle , or hydrologic cycle, is one of the most important processes to living organisms on Earth. Consider the following facts:
Signify the term - Trachea Part of the respiratory system in insects, vertebrates and some spiders. In invertebrates it comprise tubules carrying air directly from outside to t
Air pollution can be controlled by two fundamental ways. Preventive technique: Important measure to control air pollution Suitable fuel having low sulphur content shoul
Explain the Process of Canning? The basic principles of canning have not changed dramatically since Nicholas Appert and Peter Durand developed the process. Heat sufficient to d
Q. Why is it dangerous to talk while eating? If a person talks when eating, food may be inhaled into the lungs. Generally after food is swallowed, it passes into pharynx and th
Explain me the concept with diagram
What is the function of the immune system? The immune system performs exact defense against agents, the antigens, that are foreign or harmful to the body. Exogenous antigens
What is the external rigid carapace of arthropods called? Of which substance is it made? Which type of organic molecule is that substance? The external carapace of arthropods i
Although fructose does not have an aldehyde group it reacts first of all bonds of fructose was breaked by fehling solution then fructose coverted to aldehydic group then that
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