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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Post Operative Anticoagulation : Thromboembolism remains a serious complication after valve replacement. Lifelong anticoagulation is requised for all patients with a prosthetic v
Define Enrichment Media - Culture Media? It contains selective ingredients in the medium, which shifts the growth in a mixed microbial population to a particular group of micro
EGG S OF FROG This egg is round & its diameter is of 1-2 mm. It is mesolecithal & has distinct animal pole & vegital pole. The nucleus is found in the centre of the anim
1. List alternative therapies for musculoskeletal problems. 2. What is a xyrospasm? What is the Greek derivative of the word? 3. Name and describe the three types of varus con
Draw structure of ethyl ammonium with all carbons and hydrogens.
What are the stages into which photosynthesis is divided? Photosynthesis is separated into the photochemical stage, or light reactions, and the chemical stage. Photosynthesi
write about complementary genes
Which of the following does NOT contain a guanidinium group Select one: a. Urea b. arginine c. creatine d. guanidinium ion
what is amphibian
Schizocoelous pattern – Coelom There are two patterns of coelom formation in animals schizocoelous and enterocoelous. In schizocoelous pattern, two teloblasts cells (primordi
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