Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Nucleic acids, Why is the number of bonds important in nucleic acids

Why is the number of bonds important in nucleic acids

Listeriosis, Listeriosis The organisms have an extensive host range which ...

Listeriosis The organisms have an extensive host range which includes mammal, poultry, fish, crustaceans and ticks including man. The organism has been isolated from sheep, goats,

How sex occurs in bacteria, Q. How sex occurs in bacteria? Occurrence o...

Q. How sex occurs in bacteria? Occurrence of sex in bacteria was first described by Edward Tatum and Joshua Lederberg in 1946 (Nature, volume 158, page 558) who were studying m

Explain oncogenes or defective tumour suppressor genes, 1.  Explain why a g...

1.  Explain why a gain of function would be a dominant effect and a loss of function would be a recessive effect. Which types of genes are each associated with: oncogenes or defect

Asexual reproduction and cloning in plants, before stem cuttings are plante...

before stem cuttings are planted the cut end of the stem is often dipped in a hormone powder .what is the point of this?

How are the lipids classified, Q. Concerning solubility, how are the lipids...

Q. Concerning solubility, how are the lipids classified? Oils and Fats are hydrophobic molecules, i.e., they are insoluble in water and non polar. Lipids in general are molecul

Explain adverse effects of oseltamivir, Explain Adverse Effects of Oseltami...

Explain Adverse Effects of Oseltamivir  Nausea and vomiting can occur. Taking the drug with food decreases the incidence of nausea. In juvenile rats, very high doses of osel

Walrasian model, Normal 0 false false false EN-IN X-N...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Excretory system, is thare any excretory system found in echinoderms

is thare any excretory system found in echinoderms

Determine the quality of various food grains, Q. Determine the quality of v...

Q. Determine the quality of various food grains? After undertaking this activity, you will be able to: • determine the various fractions involved with the physical examinati

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd