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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
what is endosperm haustorium
Adverse effects of lamivudine Because lamivudine is also active against hepatitis B virus (HBV), HIV-positive patients with chronic HBV infection may experience a flare of hep
Q. Of what subunits are ribosomes are made? Ribosomes are made of two subunits the small subunit and the large subunit. These subunits are made of proteins and ribosomic RNA (r
You have 875 mL of a 0.37 M acetic acid solution. What volume (V) of 1.20 M NaOH solution must you add in order to prepare an acetate buffer of pH = 4.36? (The pKa of acetic acid i
Q. How can triglycerides be decreased? Triglycerides could be decreased by: - limiting foods high in fats - decreasing sugar and sugar containing foods (carbonated bevera
Q. How do biodiversity, the total number of living beings and the biomass respectively vary during the ecological succession? The Biodiversity, the biomass of an ecosystem and
describe the endoskeleton of fish?
What characteristics might a breeder select for in (i) a cereal crop, (ii) a farm animal? (i) In a cereal variety, a breeder might select for high yield; disease-, frost- or dr
Fungi are classified in their own kingdom. Into which phyla is the fungi kingdom divided? Into which of those phyla are mushrooms classified? The kingdom fungi is separated in
Q. What is folic acid? Why is the anemia caused by deficiency of folic acid known as megaloblastic anemia? The folic acid when ionized it is called folate is a coenzyme that pa
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