Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Why are the recombinant dna technology, Why are the recombinant DNA technol...

Why are the recombinant DNA technology and the nucleus transplantation technology still dangerous? A recombinant DNA technology and the nucleus transplantation technology (clon

What are primary indications for pharmacologic stress, Q. What are primary ...

Q. What are primary indications for pharmacologic stress? The primary indications for pharmacologic stress are inability to exercise or the desire to identify viable myocardium

Explain amylase, Amylase The presence of pancreatic amylase brings abou...

Amylase The presence of pancreatic amylase brings about  the breakdown  of starch  and glycogen and  its action is similar to salivary amylase. The hydrolysis of  starch and gl

What is the function of the plant cell wall, Q. What is the function of the...

Q. What is the function of the plant cell wall? The plant cell wall has protective and structural functions. It plays an significant role in the constraint of the cell size, pr

Effect on microbial growth and ecology, Effect on Microbial Growth and Ecol...

Effect on Microbial Growth and Ecology Redox potential exerts an important selective effect on the microflora of a food since it will decide the type of microorganism that can

Describe binding of two amino acid for peptide formation, How can the bindi...

How can the binding of two amino acids for the peptide formation be described? A peptide is formed when a carbon from the carboxyl group of one amino acid is linked to the nitr

What is the importance of the glucose blood level, What is the importance o...

What is the importance of the glucose blood level for human health? The glucose blood level (glycemia) must be kept normal. If it is abnormally low there is not enough glucose

Under which conditions aerobic cells carry out fermentation, Q. Under which...

Q. Under which conditions do aerobic cells carry out fermentation? Some cells that usually obtain energy from aerobic cellular respiration can perform fermentation when oxygen

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd