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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Counselling at Diagnosis? A newly diagnosed case of diabetes will have lots of questions and will be confused regarding the treatment and complications of the diabetes. It i
What is Ecology? Ecology is the field of Biology that studies the relationship among living beings and among living beings and the environment.
Where in the leaves is photosynthetic tissue often located? The major photosynthetic tissue is the photosynthetic parenchyma (also known as chlorenchyma, do not confuse with co
Explain the Process of Solubility test? This test is given by all monosaccharides and disaccharides. Polysaccharides are not soluble in cold water. Principle All monosa
Q. What are immunoglobulins? Immunoglobulin is the alternate name given to antibody and Immunoglobulins are complex proteins containing a variable portion and an invariable por
What are multiple alleles? Is there dominance in multiple alleles? Multiple alleles is the phenomenon in which the similar gene has more than two dissimilar alleles (in normal
what is the difination of osmosis
Examination is begun by placing the transducer in left parasternal region, usually in the third or fourth left intercostal space. From this position, a sector image of the heart
The main arena of various types of activities of a cell is: 1. Plasma membrane 2. Mitochondrian 3. cytoplasm 4. Nucleus Cytoplasm
Explain the Regulation of Blood Glucose Concentration? A number of mechanisms function to maintain blood glucose at remarkably constant level of 70-100 mg/dl under fasting cond
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