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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define the Techniques of Instrument Retrieval Endo Extractor (Brasseler) a. Hollow tube bonded to coronal tip of separated instrument by cyanoacrylate adhesive, with
(a) What effect does alcohol have on reaction time? (b) What other short-term effects does alcohol have? (c) What long-term effects can result from an exce
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how do viruses reproduce
Explain Gluconeogenesis Gluconeogenesis (i.e synthesis of new glucose) is the synthesis of carbohydrate from non-carbohydrate, source. The major substrates for glucone
Artificial Parthenogenesis In various cases, the eggs that normally develop only after fertilisation can be experimentally induced to develop parthenogenetically by specific
Explain the Air Samplers - Air Sampling? Air Samplers, e.g., all glass impinger and the Andersen sieve samplers may be used. Volume of the air sampled is known. In all glass im
Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiol
Phyium Oomycetes 1) They reproduced asexually by non-motile conidia and/or mobile flagellatedzoospores. 2) The sexual reproduction is by fusion of a male gamete with an egg
Diagnostic Wax Up A diagnostic wax up should be done by you or got done from a laboratory for each and every case regardless of the extent or difficulty level of the case as it
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