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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the functionality of Central Nervous System ? The central nervous system is the center of the nervous system, receiving impulses from, and sending impulses to, the per
what is example of class,genus and species of platyhelminthes?
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What is the secondary structure of a protein? The secondary protein structure is formed by the manner its amino acids interact by the intermolecular bond. These interactions ma
Define Evolution and Growth of Sports Nutrition as a Discipline? An academic discipline is basically a formal body of knowledge discovered, developed, and disseminated through
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How do embryos of placental mammals excrete nitrogen wastes? Placental animals, as well as embryos, excrete urea. In the adult placental mammal urea is excreted by the urine.
What are the possible effects on the fetus if, during pregnancy, the mother (a) smokes, (b) catches rubella? a) Smoking during pregnancy can lead to an underwe
Define Important Points While Working With Autoclave? Note: Following points should be kept in mind while working with autoclave. 1. Autoclave should not be packed tightly o
SCOPE OF BIOLOGY- Present century is witnessing explosion of information in modem biology directly related to life and health. Issues like global warming, pollution, populat
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