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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
how much classification of protozoa
Ethylene glycol is a poison that causes about fifty deaths a year in the United States. Treating people who have drunk ethylene glycol with massive doses of ethanol can save their
Excretory organ for protozoa
St. Jude Medical Valve (SJM) : This is a low profile bi-leaflet valve, which was introduced in 1977. The leaflets and the housing are made of pyrolitic carbon. The currently
What is Glycolysis in Cell Metabolism? Glycolysis : is the process that breaks down glucose into pyruvate and energy. Glycolysis does not require the presence of oxygen and
What gum conditions may result from a failure to remove plaque? Gingivitis (gum inflammation) and periodontal disease (infection of the socket) might be result from a failure t
Define Nutritional Needs of the Elderly? In this, we learnt about the nutritional needs of the elderly, and in doing so we have also focused on many other aspects such as who d
Describe the nature of the enzymes involved in the phosphorylation of glucose Phosphorylation of glucose: Glucose is converted to glucose-6-phosphate since phosphorylated inter
Q. What are plasmids? And what is the significance of plasmids for the recombinant DNA technology? Plasmids are circular fragments of DNA that are accessories to the main bacte
What are the main constituents of the earth's atmosphere in our time? The present atmosphere of the earth is constituted mostly of molecular nitrogen (N2) and molecular oxygen
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