Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Cnidarla, Tlie phylum Cnidaria or Coelenterata, includes the faliiiliar hyd...

Tlie phylum Cnidaria or Coelenterata, includes the faliiiliar hydras, jelly fishes, sea anemones and corals. You have olreacly studied about Cnidarialis in Units 4 and 7 of LSE-09

Define the reduction of the activation energy, How does the formation of th...

How does the formation of the enzyme-substrate complex define the reduction of the activation energy of chemical reactions? The enzyme possibly works as a test tube within whic

What should we focus on during pregnancy, What should we focus on During pr...

What should we focus on During pregnancy? Our focus should be strongly on the preventive aspects of nutrition, identify women at-risk, recognize special needs for counseling, a

Transparency - aquatic habitats, Transparency - Aquatic Habitats Trans...

Transparency - Aquatic Habitats Transparency affects the extent of light penetration. It is indirectly related to turbidity. Suspended particulate matters such as clay, silt a

Show the important groups of bacteria, Q. Show the important groups of bact...

Q. Show the important groups of bacteria? The important groups of bacteria are: a) Bacillus: rod-shaped. b) Coccus: spherical. c) Coccobacillus: oval-shaped. d)

Explain use of rifaximin, Rifaximin (Xifaxan)  An anon-absorbed oral an...

Rifaximin (Xifaxan)  An anon-absorbed oral antibiotic, was about as effective as ciprofloxacin for treatment of travelers' diarrhea, mostly caused by E. coli. It is not effecti

Predict the genotypes and phenotypes of the f2, In rats, the gene pigment (...

In rats, the gene pigment (P) is dominant to no pigment (p). The gene for black (B) is dominant to the gene for cream (b). If a pigment gene (P) is absent, genes B and b are inoper

Human population-historical overview, Human Population-Historical Overview ...

Human Population-Historical Overview Throughout history, the human population has been quite small. It has grown relatively slowly and even experienced occasional declines. Fi

Formation of an h+ gradient, All of the electron carrier in the electron tr...

All of the electron carrier in the electron transport chain interact according to their redox potentials.  Every time whereas an electron transfers occurs, the accepting carrier ha

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd