Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Effect of genetic drift, Is the effect of genetic drift likely to be the sa...

Is the effect of genetic drift likely to be the same in pop 1 and pop 2? How are genetic drift and pop size related? when there is strong selection against the homozygous recessive

Learning objectives of coronary artery disease, Q. Learning objectives of c...

Q. Learning objectives of coronary artery disease? • describe the various forms of coronary heart diseases, • enumerate the risk Factors (genetic and environmental) in causati

Define reagents required and methodology for fehling's test, Define Reagent...

Define Reagents required and Methodology for Fehling's Test? - Sugar solutions of glucose, fructose, galactose, lactose, maltose, sucrose, starch - Fehling A (Copper sulphat

Glycogen synthesis, To synthesize glycogen two enzymes are needed: 1.  U...

To synthesize glycogen two enzymes are needed: 1.  UDP-glucose   pyrophosphorylase catalyzes the synthesis of UDP-glucose from UTP and glucose 1-phosphate:   The   py

Define food intake during polar expeditions, Define Food Intake during Pola...

Define Food Intake during Polar Expeditions? Observations made by Easty (1967) at Halley Bay, the British Antarctic survey base during 1961-62 expeditions indicate mean calorie

How is digestion performed in protozoans, How is digestion performed in pro...

How is digestion performed in protozoans? Digestion in protozoans is intracellular digestion: organic material is internalized and degraded inside the cell. Protozoans get

What a test tube brush and how is it used, What a test tube brush and how i...

What a test tube brush and how is it used? It is a method, made with nylon bristles attached to a twisted-wire shaft, used to knock the bottoms out of test tubes.

Explain about vitamin D, Vitamin D (Calciferol - vitamin D2) The main f...

Vitamin D (Calciferol - vitamin D2) The main forms are Vitamin D2 (ergocalciferol-plant origin) and vitamin D3 (cholecalciferol-animal origin). Vitamin D2 forms colourless, aci

Drugs and insulin used in diabetes, Q. Drugs and Insulin used in diabetes? ...

Q. Drugs and Insulin used in diabetes? When diet, exercise or even weight reduction do not improve the diabetic symptoms and blood sugar levels, the uses of hypoglycemia drugs

Signify the term in details - pseudocoelom., Signify the term in details - ...

Signify the term in details - Pseudocoelom. A body cavity that isn't completely lined by mesoderm. Mesoderm is associated with only the ectodermal surface but not endoderm. The

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd