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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What are the Signs of cardiac tamponade? The clinical presentation will be that of a low output state with anxiety, restlessness, dyspnoea, sweating, cold extremities and dr
what is the normality law?
Define Essentiality of Nitrogen for Growth of Micro-Organism? Nitrogen is an essential atom in certain cellular macromolecules, e.g. amino acids, purines, pyrimidines, enzymes,
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Diagram of a cell surface membrane. State the width of a cell surface membrane. (i) On which side of the membrane, shown in Figure, X or Y, is the cytoplasm of the cell?
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