Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Explain reality theory, Reality Theory Developed in  the 1960s by  Will...

Reality Theory Developed in  the 1960s by  Willian glasser,  a  psychiatrist,  reality  therapists  view human nature in terms of behaviour. They believe that human behaviour i

Nutrition, difference between holophytic nutrition and holozoic nutrition.

difference between holophytic nutrition and holozoic nutrition.

Results-ross operation--surgical techniques, Results: The risk of isolate...

Results: The risk of isolated aortic valve replacement is 3-4 per cent. The long term survival for 5, 10 and 15years is 75 per cent, 60 per cent and 40 per cent respectively. The

Explain thermal destruction of enzymes during sterilization, Explain about ...

Explain about Thermal Destruction of Enzymes during Sterilization? Before we move on to canning, we need to emphasize here that like thermal destruction of microorganisms, ther

Results-types of surgery in ar, Results: Largely depends on the LV functi...

Results: Largely depends on the LV function. In most centres surgical mortality is 3 to 4 percent. For patients with severe LV dysfunction the immediate and late results of sur

Ethylene - plant hormones, Ethylene - Plant Hormones Amongst hormones ...

Ethylene - Plant Hormones Amongst hormones in both plant and animal kingdoms, ethylene, a gaseous hydrocarbon, is unique. Despite its chemical simplicity, it is a potent growt

Disposal of microorganisms, Disposal : You must always sterilize cultures ...

Disposal : You must always sterilize cultures and contaminated instruments and glassware before disposing them of or before washing-up. Usually, you can do this by autoclaving or

What are the results of atrial septal defect, What are the results of Atria...

What are the results of Atrial Septal Defect ? Mortality for ASD closure has approached 0 per cent in most cardiac surgery centers. Late survival of patients who has ASD closu

Zoology, regenerstion in invertebrates

regenerstion in invertebrates

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd