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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What would be the approximate number of amino acids of a protein with a mass of 203 kd? Assume an average molecular weight for amino acids of 110 grams/mol.
Q. Management of hypertension? the management of hypertension, angina pectoris and myocardial infarction. It must be clear to you that some aspects of dietary management may di
Class angiospermae (Flowering plants) Flowers are the reproductive structures. Ovules are protected within ovary, xylem vessels are present. After fertilisation the ovary develop
Q. What is facial prosthesis? Dental implants have prosthesis also been used for facial prosthesis which includes nasal, orbital or auricular prosthesis. Implant and bar retain
The human skeleton has evolved from that of four-legged animals. Unfortunately, the adaptation is far from perfect; thus, our upright posture causes problems like back age and the
Explain about Battery Battery: Two electrodes (zinc and carbon) placed in electrolyte (dilute sulfuric acid). Chemical reactions in electrolyte force positive charges to
Inversion We know that atmosphere is heated from below and warm air rises, but air can be prevented from rising by a condition known as inversion. An inversion refers to a situ
charachteristicsof phylum canidaria
What are pioneer species? What is the role of the pioneer species? Pioneer species are those first species that colonize places where before there were no living beings, like,
Explain the Bulimia Nervosa? Diagnostic Criteria Bulimia Nervosa patients, unlike those of anorexia nervosa with binge and purge subtype, are typically within the normal weight
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