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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
In biology, a species is smallest and basic unit of classification. Each species is placed within a single genus. For example biological name of human being is homosapiens. Here ho
What is the reason behind the high surface tension of water?
Q. Meaning of Counselling in diabetes mellitus? The word counselling is a very broad term which is used for helping others to overcome their particular difficulty. It has been
What are three main problems with having only two kingdoms There are three main problems with having only two kingdoms. The first problem concerns the unicellular flagellates
what are sphaerosomes?
Q. What is an antibiogram? The Antibiogram is a laboratory test intended to guide the choice of sufficient antibiotic to treat a given bacterial infection. In an Antibiogram cu
What are the differences between astral and anastral mitosis? Astral mitosis is that in which there is structure of the aster, a structure made by the centrioles. Anastral mito
Indifferences in ionic composition Answer A. across membranes can be created through the action of ATP driven pumps as long as number of negative and positive ions on remain equal
Borna disease Borna disease is fatal neurological disease of horses and sheep caused by a virus (RNA) of the family Bornaviridae and the disease is characterized by sub acute meni
Q. Describe Sampling of grains? The quality of the food grains is assessed starting with the process called sampling. A sample of the product to be evaluated is taken and the
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