Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Plant might be adapted to a hot dry environment, Give three ways in which a...

Give three ways in which a plant might be adapted to a hot dry environment. A plant might be adapted to a hot dry environment by having a decreased leaf area, waxy cuticle to

Define vaccine immunogenicity, Immunogenicity Based on immunogenicity s...

Immunogenicity Based on immunogenicity studies and experience with other vaccines, the new meningococcal conjugate vaccine (Menactra) should be highly effective in preventing m

Formation of the amino acids sequences of a protein, Q. How do nucleotides ...

Q. How do nucleotides of mRNA chains encode information for the formation of the amino acids sequences of a protein? There are only four types of nitrogen- containing bases tha

Diversity is good axioms of conservation biology, "Diversity is good" is on...

"Diversity is good" is one of the axioms of conservation biology. However in absentia of historical information, why is noting higher diversity in a system NOT the best way to eval

What is the generic function of leukocytes, What is the generic function of...

What is the generic function of leukocytes? What are leukocytosis and leukopenia? The generic function of leukocytes is to contribute in the defense of the body against strange

Deficiency diseases-predisposing factors, Predisposing factors The inc...

Predisposing factors The incidence of metabolic diseases in cows and buffaloes increases as the milk production increases and the herd size becomes larger. The highest inciden

Role of carbohydrates in dyslipidemia, Q. Role of Carbohydrates in dyslipid...

Q. Role of Carbohydrates in dyslipidemia? It is important to know about these carbohydrates, as they all differ in their digestive properties. The rate of absorption is variabl

Embryology and histology, Embryology: Embryology (from Greek words embryon...

Embryology: Embryology (from Greek words embryon which means unborn embryo and logia which means study) is a branch of science which deals with the development of an embryo starti

Infectious bronchitis (ib), I nfectious bronchitis (IB) Infectious bro...

I nfectious bronchitis (IB) Infectious bronchitis is a highly contagious respiratory disease of poultry, caused by a virus belonging to the family Coronaviridae. Its existence

Solubility of gases in water, Solubility of Gases in water Most gases w...

Solubility of Gases in water Most gases which are important for biological processes dissolve readily and specially in water. The solubility of any gas in water generally var

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd