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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Human Impact on Nitrogen Cycle Human activities are profoundly affecting the cycling of nitrogen in nature. Over 30 x 10 6 metric tons/yr. of N 2 is fixed in the commercial
Sublimation is the process by which solid water changes directly to vapour phase without passing through the intervening liquid phase. The gradual disappearance of flakes of ice
What are the Applications of Nicotinic acid Nicotinic acid is mainly used in the vitamin fortification of flour, macaroni and noodle products. Independent of its vitamin effic
What is a genotype? What is the difference between genotype and phenotype? Genotype is the genes, DNA nucleotide sequences have in the chromosomes of an individual that conditi
The intermediates in the cycle give precursors for various biosynthetic pathways. An instance is as given below: - From citrate synthesis of fatty acids; - Amino acid synth
filter feeding mode of nutrition is observed in- a) amoeba b) euglena c)paramoecium d) all of the above
The boll weevil, an insect native to Mexico and Central America, is another pest of commercial cotton. When chemical insecticides were first discovered, they proved highly effectiv
What are the main representatives of the pteridophytes? Is this plant group cryptogamic or phanerogamic? The better known pteridophytes are the ferns and the maidenhairs, from
Psychology : This is the study of human mind. On other words Psychology is the science of behavior or mental processes. Its immediate aim is to understand the individuals or groups
Define Management of Canal Blockage Canal Calcification Diamond coated instrument rt 1 designed for better visibility and cutting efficiency a) Very powerful for efficient
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