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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Physical properties of an oil Physical properties of an oil or fat are of critical importance in determining its functional characteristics or use in food products. One
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Determine the Occurrence of Vitamin E The tocopherols are widely found in animal and vegetable materials, like nuts and seeds. Considerable amounts are found in a number of
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2.?In order to increase muscular force production, muscular contractions may be modulated in two ways. Explain.
Q. Explain H - Shaped Incision? This type of incision is especially useful for: The anterior maxilla or mandible. Accurately identified implant position. Exposure o
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