Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

#title., 15 character of protozoa

15 character of protozoa

Explain group specificity, Group  specificity Some enzymes prefer a sp...

Group  specificity Some enzymes prefer a specific functional group to be present\%on  the substrate molecules. Example:- alcohol dehydrogenase  acts on alcohols having  -OH  gr

What structural features do these organisms, What STRUCTURAL features do th...

What STRUCTURAL features do these three organisms have in common in terms of a) excretory systems b) respiratory systems c) circulatory systems and d) nervous systems ? The species

What are main successful vaccines, Main successful vaccines are live attenu...

Main successful vaccines are live attenuated and heat killed vaccines.

Explain cidofovir, Cidofovir (Vistide) IV cidofovir given once weekly ...

Cidofovir (Vistide) IV cidofovir given once weekly for 2 weeks and then once every 2 weeks for maintenance therapy can delay progression of CMV retinitis in patients with AIDS

Phototropic curvature is the result of uneven distribution, Phototropic cur...

Phototropic curvature is the result of uneven distribution of : 1. Gibberellin 2. Phytochrome 3. Cytokinins 4. Auxin Auxin

Oxygen cycle, Oxygen is a major component of all living organisms. All the ...

Oxygen is a major component of all living organisms. All the organisms and plants required oxygen for aerobic respiration which they absorb from environment. Only plants release ox

Enzyme - linked immunosorbent assay (elisa), E n z y m e - l...

E n z y m e - linked immunosorbent assay (ELISA):   ELISA in its var io us modifications e.g. plate ELISA, sandwich ELISA, competitive ELISA, ELISA strip or

Explain about freezing point - properties of solutions, Freezing Point - Pr...

Freezing Point - Properties of Solutions The freezing point of a material is the temperature at which it changes from a liquid to a solid. A liquid freezes when its vapour pre

Bones, which bone forms the non-moving muscle attachment in the hip joint

which bone forms the non-moving muscle attachment in the hip joint

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd