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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Can you explain Toxicants in Plant Foods? Several foods, some of which we consume regularly, have toxic compounds in them which are best avoided. These compounds can be prot
Vegetative Development - Growth It is quantitative and irreversible change. It reflects an increase in size and volume of an organism. For example, the change in the size of a
What are the parasympathetic neurons Patient A has a disease that has destroyed half of the alpha-adrenergic receptors on each of the smooth muscles that control the diameter o
Abortions Abortion in dairy cattle is commonly defined as a loss of the foetus between the age of 42 days and approximately 260 days (buffaloes 300 days). It is important to d
Q. What are the main functions of the cytoskeleton? As the name indicates, the cytoskeleton is accountable for the support of the normal shape of the cell it also acts as a fac
Full Description about microfilament
Egg - Synergids The three cells of the egg apparatus are arranged in triangular fashion with the egg sharing a common wall with the two synergids and the central cell. In the
A large number of plants are used for bio-pesticides and bio-fertilizers. Several plant species are used as insecticides against aphids and larvae of insect pests: for insects like
Q. Do sponges have circulatory, nervous and excretory systems? Sponges do not have a nervous system neither excretory system nor circulatory system.
brief account on classification and general characters of protozoa
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