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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
15 character of protozoa
Fibroids, also called as leiomyomas, are benign smooth muscle tumors that happen in the uterus. They grow under the influence of estrogen and progesterone, and are therefore seen i
What is the basic morphology of a protozoan cell? Protozoans are eukaryotic cells so they have organelles and structures common to this part of cell: endoplasmic reticula, Gol
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Define the Concepts of Lipids? You have studied the classification of lipids in your theory lesson. Lipids, you learnt, are classified as simple, compound or derived lipids on
Phospholipids exhibit important biological functions. They are: a) increase the rate of fatty acid oxidation b) act as inorganic ion carrier across the membrane c) hel
Plankton - Aquatic Ecosystem This group includes both microscopic plants (phytoplankton) and animals (zooplankton) found in all aquatic ecosystems, except certain swift moving
how do carnivorous feed
Ciliated Protozoan Ciliates form the largest and the most homogenous group of protozoans. They are placed under one phylum Ciliophora and evidence indicates that its members s
Calculate a 95% confidence interval for the difference among the average numbers of ant species in the two regions. Compare your results to SPSS output and interpret the confidence
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