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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Debate over ethics of using gene therapy for treating human beings started since the discovery of recombinant DNA technology. The mere fact that it involves the alteration of DNA w
Define Diet for Treatment for Protein Energy Malnutrition? Treatment of cases of kwashiorkor or marasmus involves mainly providing appropriate nutrition support. The child shou
Acute Myocardial Infarction : Patients with acute non-Q myocardial infarction may need urgent intervention as indicated for cases of unstable angina.
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Bacterial food poisoning Food poisoning is an acute gastro-enteritis or intestinal disorder resulting from the ingestion of foods contaminated with either living microorganism
Heartwood differs from sapwood in: 1. Presence of rays and fibres 2. Absence of vessels and parenchyma 3. Having dead and non-conducting elements 4. Being susceptible t
Maintenance and Regulation of Peripheral Circulation Pressure receptors (baroreceptors) located in the wall of the internal carotid arteries and in the arch of aorta, when
Q. What is viscosity? Viscosity is resistance to flow of a liquid. It is a measure of the resistance of a fluid to deformation under shear stress. It is commonly perceived as "
Explain ozone shield? Name two gases that can cause damage to this shield. Give one harmful effect of this damage each on plants and animals.
FATS ( = True Fats, Triglycerides, Neutral Fats) They are esters of fatty acids and 3-carbon trihydric alcohol glycerol (= glycerine). T rue fat is also called trig
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