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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Insulators Numerous experiments showed that a transgene is generally poorly expressed when it contains a cDNA rather than the corresponding genomic DNA; when it is integrated a
Q. How different is the fish epidermis from the amphibian epidermis? The fish epidermis is very contains and thin mucus-secreting cells the fish skin doesn't present keratin, t
Why acquired traits are not directly related to the process of evolution? As acquired traits are not genetically determined, they cannot be passed on to offspring. Therefore,
Synapse Nervous System Structural feature of unique importance in the nervous tissue is the synapse. A synapse is the anatomical site at which axon terminal processes of one
Purple (P) flowers are dominant and white (p) flowers are recessive. A homozygous dominant purple flower is crossed with a homozygous recessive white flower. what percentage of the
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What are the chemical substances that compose the plasma membrane? Ans) The major constituents of the plasma membrane are phospholipids, proteins and carbohydrates. The phosphol
MERIT AND DEMERIT OF FERTILITY MEASUREMENT
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Ears We have a pair of ears. Each ear has three parts - external, middle and inner ear. The function of the middle ear is transmission of sound waves from external to inner ear
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