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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Use of Chemicals to Control and Destruct Microorganisms? Ans. The use of chemicals, as a process of food preservation, has been used since long, ever since man found
Determine the significance of Siphuncle. A cord of tissue that passes between different chambers of a Nautilid shell. Siphuncle regulates the amount of air or fluid in chambers
Your client has the flu and reports 5-6 loose stools a day. He has experienced an isotonic fluid volume loss. Explain what an isotonic fluid loss means. Your patient has a respi
What is the first step normally taken when you look through the oculars? The nuclei of the cheek cells have been stained using a special dye so that they appear purple. What is the
Suppose you were breeding rabbits and wanted to design an experiment to see if a new type of food would help your rabbits have fluffier fur. How would you treat the rabbits in the
in sericulture industry do which stages of silkworm weaver buy? why do they do so?
Question 1 A patient is admitted to the hospital with suspected pneumococcal pneumonia. How would you confirm this initial diagnosis? Answer the following questions a) Which is
#question.why obelia is considered of special interest in zoology.
Q. What are the endocrine functions of the placenta? The placenta has endocrine function since it secretes the hormones estrogen and progesterone that maintain the endometrium
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