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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is the estimated percentage (in mass) of water in the human body? Is this percentage expected to be larger in the adult or in the old individual? Approximately 65% of t
Q. What is the DNA vaccine? The DNA vaccination or is a vaccination technology based on genetic engineering. In DNA vaccine a recombinant plasmid (vector) containing the gene o
Phospholipids exhibit important biological functions. They are: a) increase the rate of fatty acid oxidation b) act as inorganic ion carrier across the membrane c) hel
Titration of inverted honey solution It involves the titration of the inverted honey solution. Carry out the titration in the following manner. 1) In a conical flask take 5
Explain functional properties of gellan gums One of the most important features of gellan gums is its versatile texture which is defined in terms of hardness (measure of ruptur
Why should you not apply cosmetics while doing a lab experiment in class?
What are examples of nematodes? Ascaris, hookworm and filaria, all parasites of humans, are instance of nematodes (also called as roundworms).
PHYLUM CNIDARIA (= COELENTERATA ) Definition and Introduction Tissue grade eumetazoans with a radial symmetry .The term coelenterate signifies the presence
What is mitochondrial inheritance? The Mitochondrial inheritance is the passage of mitochondrial DNA molecules (mtDNA) to the offspring. All the stock of mtDNA an individual ha
What is the rule for the pairing of nitrogen-containing bases in the DNA molecule? And in the RNA? The rule for the pairing of nitrogen-containing bases of the polynucleotide c
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