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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Corelation of ST Depressoin with Coronary Angiography? Various investigators considered 1 mm of horizontal or downsloping ST depression to denote a positive test and used fr
Q. What are the main biological functions of water? Ans. Water is the basic solvent for chemical reactions of living beings; it is the main means of substance transportati
Suppose that there are six pairs of alleles which control height and that every dominant allele adds 5cm to the stature. Assume also that the average height of an adult (with equal
What are muscle tissues? How is the function of this tissue related to the typical characteristics of its cells? Muscle tissues are tissues made of cells able to perform contr
Explain Precautions for Morphological Study of Fungi? 1. Use sterile forcep and needles for slide culture technique. 2. Aseptic conditions should be maintained. 3. Caref
Explain Results of abdominal aneurysm surgery? Results: For ascending aorta replacement, the hospital mortality varies from 0 to 9 per cent. Aortic arch has higher risk of
Define the role of Fluorine in Human Body? Fluorine is potentially a toxic element. Its essentiality for humans is not established although the role of fluoride in providing pr
Surface area The rate of oxidation increases in direct proportion to the surface area of the lipid exposed to air. Furthermore, as surface - volume ratio is increased; a given
Define Advantages of Using the Plate Counts Method? 1. It is simple and sensitive method. 2. It provides viable count as only live cells will grow and produce the colony.
Give two examples of chemical reactions which are catalysed by enzymes in the course of brewing. In the course of brewing, enzymes in the grain catalyse the conversion of star
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