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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Goals of prevention of diabetes mellitus? The goals of prevention are to delay the onset of DM and preventing or delaying the cardiovascular complications. Obesity should be
It corresponds to the anterior and inferior interventricular grooves. Its right side is convex and bulges to the right ventricle. The septum has a posterior border which separates
The main objectives of study of ecology are: (i) To study the distribution of biota local and geographical level. (ii) To study the interrelationship
Explain about the Adequate Intake (AI)? If sufficient data are not available to establish an EAR and hence RDA, the AI is derived instead. The AI is derived from observations o
X-Linked Traits in Humans Let us first examine traits determined by genes on the X-chromosome, or the X-linked traits. The human X chromosome contains many genes that are requi
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Why doesn't a long lasting vaccine against common cold exist yet? Viruses that show a high mutation rate like the virus that causes the common cold escape simply from the actio
Which is better to grow plants in Rock sand or soil? When we grew plants inside, with there is no wind, and the plants in the rocks grew improved than the plants that were grow
Explain Oedema - Clinical Signs of Kwashiorkor? Oedema: Oedema refers to accumulation of fluid in the tissues and usually begins with a slight swelling in feet gradually spread
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