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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Question 1 How would you perform ABO blood grouping? Add a note on advantages and disadvantages of each method. Also discuss the precautions you may take to avoid errors in variou
Brooke's Formula: a) Fluid requirement b) Estimate the accurate/approximate weight of the patient c) First 24 hours Colloids (blood, plasma, dextran) 0.5 ml
Directly observed therapy In treating TB, poor adherence to therapy is the most important cause of treatment failure and is associated with emergence of drug resistance. Medica
Fowl pox Fowl-pox is a highly infectious disease of poultry, especially chickens and turkeys. Pheasants, ducks, geese and guineafowls also suffer from an infection similar to f
Based on the scientific name. Streptococcus agalactiae what morphology would you expect these cells to have?
Reparative Regeneration This type of regeneration, as the name suggests, involves repair of a wound or replacement of a body part removed intentionally or because of injury.
how many hair are there on head
What are nymph and imago? Nymphs are larvae of hemimetabolic insects (like grasshoppers). They are very same to the adult insect although smaller. In holometabolic insects (lik
What is risk factor interaction ? Coronary artery disease, as has been explained, is a multifactorial disease with diverse risk factors coming together and interacting to produ
Explain Prophylaxis of lyme disease No vaccine is currently available to prevent Lyme disease in humans. Avoidance of ticks and use of tick repellents such as DEET or picaridin
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