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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
the s phase of interface is important and a cell can newer divide without it justify?
Explain Back pain, arthritis and gout - Effect of Obesity? Abdominal obesity increases the risk of back pain because of the extra load on the spinal column. This, in turn, red
MCNAUGHTON'S CASE AND LAW: In 1843, McNaughton shot and killed Sir Robert Peel's secretary Edward -Drymmond. It was shown that McNaughton suffered from a number of delusi
Q. What are the functions of the musculoskeletal system? The musculoskeletal system has the functions of protecting and supporting organs, maintenance of the body spatial confo
The foods we take have three basic ingredients - carbohydrate, protein and fat. Insulin has profound effects on the metabolisms of carbohydrate, protein and fat. Metabolism helps o
applications of diffusion
are viruses cellular organisms
Q. Which respiratory adaptations or organs do aquatic and terrestrial arthropods respectively present? In crustaceans, typical aquatic beings, there are richly vascularized gil
Vitamin B Complex The vitamin B complex comprises the vitamins B 1 , B 2 , B 6 and B 12 as well as the vitamin B factors biotin, folic acid, nicotinic acid and its amide as w
SPECIATION - Origin of new species: An isolated population of a species independently develops different types of mutations. The later accumulate in its gene pool. Afte
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