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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
The most recent blood work of a patient with a diagnosis of acute myelogenous leukemia (AML) reveals thrombocytopenia. Where is the patient most likely to experience abnormal bleed
Q. In which meiotic division does the separation of identical chromatids take place? And After the end of this process what are the ploidies of the new cells? The separation of
Q. Dietary recommendations proposed by WHOM? We learnt about their etiological factors, metabolic alterations, clinical anifestations and dietary management of the disease of h
Define Counter Stain - Staining Technique? Finally, the smear is counter stained with a simple basic dye different in colour from crystal violet. Safranin is the most commonly
Explain Simple Staining Technique? Here single staining agent is used to determine the size, shape and arrangement of bacterial cells. It is simple and easy to perform. Dried s
Many definitions of species have been offered, but none of them proved to be satisfactory. The definitions did not categorically provide the basis to decide whether two similar gro
Define crucial periods of a woman's life - pregnancy and lactation? In this unit, we studied about the most crucial periods of a woman's life, pregnancy and lactation, especial
Why is it significant that exact copies of DNA are produced during replication? Producing exact copies make sure that when a cell divides, the offspring cells will receive the
Most forms of cardiac diseases, like hypertension, valvular diseases and coronary artery diseases manifest as low output heart failure. Systemic vasoconstriction with cold extremit
Why is complementary base pairing important in DNA structure? Complementary base pairing is important because the hydrogen bonds between the bases hold the two strands of DNA
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