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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the groups of diabetes food pyramid The six groups of diabetes food pyramid are shown in Figure below. 1) Fat, oils and sweets (smallest group) 2) Milk 3) Mea
Explain Precautions for capsule staining in a culture? 1. Never heat fix the smear. This is because by heating shrinkage can occur which may create a clear zone around the cell
Silviculture: It concerned with the cultivation of timber yielding plants. Silviculture is the way of controlling the growth, establishment, composition, health or quality of fore
Q. What are the classes of lipoproteins in the blood? Blood lipids (cholesterol, triglycerides and phospholipids) being insoluble in blood need a ship to travel in our body; it
PRINCIPLES OF DARWINISM - 1 . OVERPRODUCTION (PRODIGALITY) - All organisms have enormous fertility and they multiply in geometric ratio. Examples of over product
one disadvantage of the pyramid if a tree and grass each count as one organism
Which of the following is a true statement regarding the Eyes Absent Protein? A. EYA is a transcriptional activator that having both a DNA binding domain and a transactivation
Chronic Renal Failure (CFW) Chronic renal failure begins when the diseased kidney can no longer maintain the normal chemical structure of body fluids under normal conditio
Explain the Food Applications of Gum Arabic A major use for gum Arabic is in the confectionery industry where it has two important functions; to retard or to prevent crysta
Photoorganoheterotrophs and Chemoorganoheterotrophs - Nutritional Types (1) Photoorganoheterotrophs - These microorganisms use light as a source of energy and organic compound
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