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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
DEFECT S OF HEART 1 . Blue Baby syndrome (Cyanosis) - Due to persisting foramen ovalis in atrial septum even after birth, the impure blood from right auricles comes to
What would you conclude from PCR/DGGE (denaturing gradient gel electrophoresis) analysis of a sample that yielded one band by PCR and one band with DGGE? One band by PCR and two ba
The Father of Botany (370-285 B.C.); of all the men who ever lived upon the earth certainly one of the most remarkable was Theophrastus of Eresus who was born about 370 B.C. on the
Q. Pathophysiology of Tricuspid regurgitation? Tricuspid regurgitation is associated with prominent venous filling waves and elevated right atrial venous pressures. Hepatic and
Data Gathering It is the gathering of information about client's physical heath, mental outlook, social circumstances, family and community life, and other factors. To p
How are human voices created? Human voice is formed with the help of muscles in the neck and the vocal cords. The tighter the vocal cords the higher the pitch of voice. This is
Shell - body form Protozoans Majority of protozoans posses an exoskeleton in the form of a shell, also called test. The shell forms a loose covering around the body of the ani
Determine the stages of healing events The healing events are described in 3 stages Stage 1: Wound healing and formation of woven bone (callus) (2 to 6 weeks) Stage 2: La
#question.why are active cells small .
Preparation of ICU Cardiac surgical ICU is generally connected to the operation theatre so that the patient can be wheeled into the ICU after surgery and also to wheel back
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