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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Awareness for diabetes mellitus? Awareness should be generated in the community to prevent the diabetes mellitus, since we know prevention is better then cure. Use behav
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Q. What is Forebrain? Forebrain: Found in the area of the forehead, this part of the brain is concerned with all the emotions, planning, organising, reasoning, memory, movement
Explain Results of abdominal aneurysm surgery? Results: For ascending aorta replacement, the hospital mortality varies from 0 to 9 per cent. Aortic arch has higher risk of
Which one of the following statements about morula in humans is correct? 1. It has almost equal quantity of cytoplasm as an uncleaved zygote but much more DNA 2. It has far l
Arrayed library: An individual primary recombinant clones (hosted in the phage, cosmid, YAC, or other vector) which are placed in two- dimensional arrays in microtiter dishes. Eac
Name the Associated Foods used in bacillus cereus Cereal dishes that contain corn and corn starch, mashed potatoes, vegetables, minced meat, liver sausage, milk, cooked meat.
Is there any situation in which DNA is made based on a RNA template? What is the enzyme included? The process in which DNA is synthesized having as template a RNA chain is know
The long term adaptive mechanisms involve myocardial hypertrophy and remodeling which occurs slowly over weeks to months. The capacity of these adaptive mechanisms meant to sust
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