Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What are enzyme cofactors, What are enzyme cofactors? Some enzymes requ...

What are enzyme cofactors? Some enzymes require other associated molecules to work. These molecules are known as enzyme cofactors and they can be, for example, organic ions lik

Protozoa, habitat of protozoa .

habitat of protozoa .

Eggs of hen, EGG S OF HEN It is a meiolecithal egg which is oval. T...

EGG S OF HEN It is a meiolecithal egg which is oval. The animal pole is found in the form of a small germinal disc. The vegital pole has two types of yolk viz. White yol

Explain the treatment of travelers diarrhea, Treatment of travelers'diarrhe...

Treatment of travelers'diarrhea  The most common cause of travelers' diarrhea, usually a self-limited illness lasting several days, is infection with noninvasive enterotoxigeni

What are the sources of nutrients of cornea, What are the Sources of Nutrie...

What are the Sources of Nutrients of Cornea? Sources of Nutrients of Cornea: 1) Oxygen for the endothelium comes from aqueous humour and for epithelium from atmosphere th

Determine the term clade, Determine the term Clade? In cladistic taxono...

Determine the term Clade? In cladistic taxonomy, how groups of animals are related to each other. It includes the ancestral group and all of the other groups, or lineages, of a

Determine the categories of latent squint, Determine the categories of Late...

Determine the categories of Latent squint Latent squint category there are five subtypes: 1)  Esophoria 2)  Exophoria 3)  Hypophoria 4)  Hyperphoria 5)  Cyclopho

#title.Homework., Why do you think it is necessary for physicians to have s...

Why do you think it is necessary for physicians to have so many different terms that describe fractures (compound, comminuted, transverse, greenstick, etc.)?

Striped muscles, STRIPED MUSCLES - These muscles are found in the li...

STRIPED MUSCLES - These muscles are found in the limbs, body walls, tongue, pharynx and beginning of oesophagus and are under the control of animal's will. These muscle f

Explain spray-dried milk powder, Q. Explain Spray-dried milk powder? Th...

Q. Explain Spray-dried milk powder? The milk which is concentrated by the process of spray drying contains about 40- 45% total solids. Do you know how the dried milk powder i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd