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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Where in the body is the thyroid gland located? The thyroid is located in the anterior cervical region (frontal neck), in front of the trachea and just below the larynx. It is
Q. How Tyndallisation used for sterilization? John Tyndall devised a process of sterilisation by steaming for a few minutes at 100 o C on 3-4 successive operations separated by
Types of Leukemia Leukaemia may be chronic or acute and is classified into the following categories: i) Acute Lymphocytic Leukaemia (ALL) This is the most comm
what is known as respiration in animals?
Explain Bone Healing Process in Nutritional Care? Protein is essential for callus formation, calcification and bone healing especially in cases of orthopaedic surgery. The prot
From which part of the teeth is it particularly important to remove plaque. It is most significant to remove plaque from among the teeth and from the region where the gum cover
what is the effect of KClO4 on the kidneys in the nephrotic system?
Infection Due to the many invasive monitoring techniques, ET tube, urinary catheter, surgical interventions. Cultures of blood or sputum/urine/swab form the wound is don
Explain Basal Metabolism Rate (BMR) - Ageing? From age 25 years, the basal metabolism decreases by about 2 percent for each decade due to the increasing proportion of body fat
a) Why is it significant that ions being transported across a cell membrane be shielded from the interior of the lipid bilayer? b) Why is photosynthesis referred to as
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