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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
elementatr cannal and feeding mechenism
Cheese Production Cheese production involves the conversion of the milk protein, k-casein to paracasein by a defined, limited hydrolysis, catalysed by chymosin (rennin). In the
Q. Classification of plants and animals? The first and foremost step in the classification of plants and animals is the. grouping together of individual organisms on the basis
Q. How to investigate aortic stenosis by Roentgenogram? Cardiac enlargement does not occur in pure compensated aortic stenosis. Post stenotic dilatation of aorta may be seen. F
what are the character selection criteria?
β - Pleated Sheets In this inter molecular hydrogen bonds are present Two or more polypeptide chains come together and from a sheet. Condensation in little. However
case study haematology
Difference between rods and cones - Rods Cones 1. More in number 1150 lakh 2. Outer seg. is cylindrical and contains
REGULATION OF BLOOD GLUCOSE LEVEL Various levels of regulation are exerted at substrate level, hormonal level, enzymatic level and at organ level on carbohydrate metabolism s
Left Antero Lateral Thoracotomy Approach : Arterial and central venous pressure monitoring lines are placed. A left antero lateral thoracotomy is done through the 5th or 4th
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