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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Procedure for Isolation of Pure Culture? Now carry out the exercise following the steps enumerated herewith. 1. Label the name of the organism on the bottom of the p
Q. Etiological factor of gastritis? They are same as acute. Generally acute gastritis if well treated gets healed in 3-4 days, however if untreated can progress to chronic gast
Acute Aortic Regurgitation : The most dramatic presentation occurs in dissection of the aortic root. Endocarditis of the native or prosthetic aortic valve may also lead to a
Iron Deficiency Anaemia This is the most common nutritional and haematologic disorder in infancy and chhildhood in developing countries. It is caused by lack of sufficient i
microorganism
Question Write a short note on the following - 1 Plastids 2 Phagocytosis 3 Ribosomal subunits 4 Microfilaments 5 Cell cycle control 6 Tight junctions 7 W
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Define about the Environmental deficiency of iodine? The ultimate causative Factor is deficient intake of iodine. Iodine occurs in soil and sea as iodide, the ions of which arc
Q. Alcohol consumption by diabetes patient? Intake of alcohol should be limited. It is high in calories, lacks essential nutrients and may therefore promote ketoacidosis, hyper
What are the main cellular features of fungi? There are unicellular and pluricellular fungi. All fungi are eukaryotes and heterotrophs. Fungi have cells with cell wall made
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