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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Food Sources of lron? lron is found in foods in one of the two forms i.e. haem or non-haem. In the human diet, the primary sources of haem iron are the haemoglobin and
Syphilis and HIV Most clinicians treat HIV-infected patients with syphilis and normal CSF with standard penicillin doses for the stage of syphilis, but some patients may need
the body cavity in roundworms is
Nitrogen Control of Nitrogen Assimilation N 2 -fixer like Klebsiella pneumoniae and Nostoc can grow with N 2 , NO - 3 or NH + 4 as nitrogen source. You would like to know
describe the cell theory and its exception
what are four types of microorganisms and their definitions?
Write the chain structure of D galactose and D mannose? The ring form of ribose is a component of ribonucleic acid (RNA). Deoxyribose, which is missing an oxygen at positi
Q. What is the formula of the DPD for withered (shrunken) plant cells and how is that situation possible? Withered plant cells are those that have shrunk due to loss of water b
Q. What do you understand by Herbarium ethics? You should also know that herbaria provide us with policy statements including such information as 1) filing arrangements, 2) res
What are the main structures within the cell nucleus? Within the cell nucleus the major structures are: the nucleolus, an optically dense region, spherical shaped, where there
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