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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Valvular Heart Diseases Normal heart valves function to maintain a uni-directional flow of blood through cardiac chambers. Two basic problems that compromise the normal functi
Transferring the Patient form ICU to Ward The patient is made to ambulate in ICU before shifting to ward . vitals are stabilized. The ward nurse is explained the pa
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Epimorphic Regeneration In this sort of regeneration the lost part is reformed and restored via the growth of a bud or blastema from the remaining part of the organism followe
The Exine Layer It consists of two layers, inner (nexine) which is continuous and an outer (sexine) the sculptured one. The outer layer has ornamentations comprising rod-like
Human Population-Historical Overview Throughout history, the human population has been quite small. It has grown relatively slowly and even experienced occasional declines. Fi
Q. How can the hypothesis that asserts that chloroplasts as well as mitochondria were primitive prokaryotes that related in mutualism with primitive anaerobic eukaryotic cells be c
ARTERIAL SYSTEM OF MAMMAL Basic plan from origin to distribution is similar in all vertebrates as per the mode of respiration. Main anterior aorta in terrestrial forms ar
To be in good health, we need to take food. Energy is provided by food to carry out activities in day to day life. In the same way, a diabetic patient also needs healthy food for e
Aim: To prove that Carbon dioxide is essential for photosynthesis. Apparatus: A potted healthy plant with long and narrow leaves, a wide mouthed glass bottle, split cork. Chemicals
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