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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Describe the Planes of Muscles The planes of superior and inferior recti in primary position form an angle of about 23 0 with the Y-axis. Therefore, the axis of rotation of th
Pachytene: The pairing of homologous chromosomes is completed. Chromosomes become more and more thick. Each bivalent has four chromatids , which wrap around each o
Q. Does each amino acid have a central carbon? To which organic group is that central carbon bound? A carboxyl group -NH 2 , an atom of hydrogen - COOH, an amine group-H and a
Adverse Effects of Imiquimod Application site reactions (irritation, pruritus, flaking, erosion) are generally mild to moderate in intensity and resolve within 2 weeks of cessa
Q. What is the usual morphological feature of nematodes that differentiates them from platyhelminthes? Nematodes are also called as roundworms. As the name indicates they are n
how we attempt a question of phylum annilida in exam?
Q. Are hormones only proteins? Some hormones are proteins, like glucagon, insulin and ADH others are derived from proteins (modified amino acids) like noradrenaline and adrenal
Management of waste amalgam and wastes containing mercury: As all of us know that amalgam is used for restorations very widely.An operator of a dental operation that produces w
What is the essential condition for a protein to be identical to another protein? For a protein to be identical to another protein it is essential for the sequence of amino aci
Quaternary Structure (4 o Structure). Globular in structure. When two or more than two molecules of protein of tertiary structure are connected to each other through
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