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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Poverty and ignorance - cause of anaemia is dietary? Low purchasing power of the communities and their consequent inability to meet the nutrient requirements, even after
Define Functions of fluoride? The only beneficial role demonstrated for fluoride is in reducing the prevalence and severity of dental caries in children and adults. This is en
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Q. What is the relation between the concepts of chromosome and chromatin? Are heterochromatin and euchromatin part of chromosomes? Every filament of chromatin is a complete DNA
Multicausal Nature of Cancer With several dividing cells in the organism and the various proto-oncogenes and tumor suppressor genes that can be changed to cause cancer. one m
Q. What is the difference between cellular specific immune response and humoral specific immune response? Humoral specific immune response is the defense system by means of, an
Phyium Oomycetes 1) They reproduced asexually by non-motile conidia and/or mobile flagellatedzoospores. 2) The sexual reproduction is by fusion of a male gamete with an egg
Preadmission Preparation Preadmission preparation is most effective when the admission is planned and there is enough time for the nurse to provide necessary information to
Planning the Nursing Care Provide bed rest Administer antibiotics as advised Prevent infection Implementation of Nursing Care Provide Bed Rest Sin
Soil-Environmental Components The word soil is derived from Latin word 'solum' meaning earthy material in which plants grow. The soil is the consolidated outer layer of the ear
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