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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
A leaf is detached from a tree and tested with iodine. The leaf turns dark blue. (a) What does this result tell you? (b) Why is this result not sufficient e
Transplantation - It is implanting of a living or preserved tissue or organ from one site to another side in the same individual or from one individual called donor to recip
Which physical elements contributed to the great amount of available energy on the primitive earth at the time of the origin of life? 3.5 billion years since the water cycle wa
Effects on Materials - Air pollutants Most air pollutants are reactive chemicals, so they react with most of the substances around. You may recall from your chemistry lessons
Gibberellins in plant growth Gibberellins were first isolated from the culture of a fungus Gibberella fujikuori. Gibberellins has a significant effect on stem elongation
What is the difference between the endocrine gland and the exocrine gland? Endocrine gland is a gland whose secretions (known as hormones) are collected by the blood and reach
Define the Drug and Drug Effect? In the discussions above, we reviewed the effect of food on drug metabolism. Interestingly, not only can drugs interact with food and alcohol,
Myocarditis Myocarditis is inflammation of the myocardium, of the heart muscles. i) Causes Infection: . Wrus, bacteria, fungus, parasites Non-infection: Radi
Q. Show the Artificial Keys? These are based on the resemblances and differences of some prominent characters in the taxa. These are based on the phylogenetic relationship b
Explain Electron transport chain Electron transport chain :Transport of high-energy electrons through a series of carriers in mitochondria.
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