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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is Photosynthesis ? Photosynthesis is the method by which plants trap radiant energy from the sun and convert the energy into a biochemical form. This biochemical energy
briefly explain filter feeding in holozoicanimals
you are setting up a pcr reaction to amplify a 1000 basepair long dna grament. in your 50 microliter reaction volume you have 100 nanogrM OD RWMPLrw sn QIRH eoufly 50/50 at/gc 100
Q. Are gametes always made by meiosis? In the haplontic haplobiontic life cycle and in the plant life cycle (diplobiontic life cycle) gametes are made by mitosis and not by mei
Q. What are the major divisions of the hypophysis? What are their functions? The hypophysis is divided into two portions- the anterior hypophysis or adenohypophysis and the pos
Explain process of Fatty acid composition We know fats/oils are made up of fatty acids. The number, position and geometry of double bonds within the fatty acids affect the rate
A protein is normally found completely within the inside of a lipid bilayer. The protein likely has: a) Mostly hydrophobic side chains, pointed outwards, with the peptide backbo
Explain the Dietary Management of a lactating mother? Meeting the needs of lactation requires a varied nutrient-dense diet. Generally, a well-balanced diet will meet nutrient n
In humans, the recessive allele that causes a form of red-green colour blindness (c) is found on the X chromosome. a) Determine the genotypes and phenotypes of the F1 generation fr
Why in the preparation of a karyotype analysis is the use of a substance like colchicine interesting? Colchicine is a substance that disallows the formation of microtubules and
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