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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Headache Associated with Neurological Diseases Headache is a symptom of many nervous system disorders, usually resulting from the distortion of pain sensitive structures. Commo
What is plant transpiration? What are the two main types of plant transpiration process? Which of them is more significant in volume? Transpiration is the loss of water from th
Define Evolution and Growth of Sports Nutrition as a Discipline? An academic discipline is basically a formal body of knowledge discovered, developed, and disseminated through
Q. List the stages of process of behaviour change? Stages of behaviour change process: · Unawareness · Awareness · Acquiring knowledge · Motivation · Trial
PHYLUM PROTOZOA Definition and Introduction All unicellular ( or acellular ) eukaryotic animals. Most primitive (Gr. Protos = first=zoon= animals ) organisms
Explain about the plateau effect - Energy Balance? You must have noticed that when people start following weight reducing diets, they lose weight rapidly in the beginning, then
Q. Define the term Behaviour Change Communication? Behaviour Change Communication (BCC) is an interactive process with communities to develop specific messages and methods usin
An insect collecting net A useful insect net can be made from a round stick such as a broom or mop handle, some heavy wire and mosquito netting or cheese cloth. Bend a heavy pi
Describe a health issue or disease and recommend an intervention (you may utilize the health issue . Justify the intervention level (primary, secondary and tertiary) and setting ch
How long do flowers usually last? Some flowers, like Bachelors Buttons, Zinnias, Marigolds, and Petunias last pretty much from the time they begin blooming until frost. Others,
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