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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the Principle or Theory of biuret method? If a strongly alkaline solution of Biuret is heated with very dilute copper sulphate, a violet colour is obtained. The substan
Term Translation or protein synthesis, is quite similar in eukaryotes and prokaryotes. We will look at the details of the process in prokaryotes and consider the differences in euk
What are abiotic factors? The Abiotic factors are the nonliving elements that constitute a given environment, like light, temperature, water, minerals, gases, atmospheric press
Types of Ovarian Cycles in Mammals Mammals exhibit two types of ovarian cycles; Estrous Cycle, exhibited by non-primates such as rats, cats, dogs, pigs, and Menstr
Q. Are there any bacteria made of more than one cell? There are no pluricellular bacteria. All bacteria are unicellular prokaryotic.
CONNECTIV E TISSUE Connective tissues are formed by the mesoderm of the embryo. Three components are present in the connective tissues- (i) intercellular medium
Hom o sapiens sapiens (MODER N MAN) - Developed from Cro-Magnon about 10,000 years ago after last glacial period in the regions of Caspian and Mediterranean seas.
Name the subcellular particle where the electron transport chain is located. Mitochondria is the subcellular particle where the electron transport chain is located.
Q. Carbohydrates requirements for ulcerative colitis? Carbohydrates: They form the easily absorbable source of energy. Bulk-producing vegetables are restricted so as to allow b
Dyslipidemia is an important correctable factor for Coronary Artery Disease. There is a strong, independent, continuous, and graded relation between total cholesterol (TC) or low-
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