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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
hi there,i would like u to help me with understanding Hutchinson phyletic dieta
Compare and contrast replication, transcription, and translation based on the following criteria. Criteria Replication Transcription
What are the typical vegetation and the typical fauna of the taigas? Taiga, or the boreal forest, is characterized by coniferous trees, pine forests. There are also mosses, lic
Defone Economic consequences of malnutrition? Figure explains the economic consequences of malnutrition. You would note from the Figure that the economic productivity of the in
TYPES OF CLEAVAGE - Depending upon the distribution & amount of yolk, cleavage is of following types - 1 . HOLOBLASTIC EQUAL CLEAVAGE ( holos , whole ; blastos, ger
Which one of the following is used as vector for cloning genes into higher organisms ? 1. Baculovirus 2. Salmonella typhimurium 3. Rhizopus nigricans 4. Retrovirus
What is the Still Retorts? Still Retorts are used in canning plants for heating foods in glass containers or different sizes of cans. These retorts are usually cylindrical in s
Impoundments - Lentic Ecosystems We have so far discussed natural lakes. In addition to these there are a number of lakes both small and large artificially created by man cal
what is respiratory system ?
R e troviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transf
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