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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Codon is the group of three consecutive nucleotides within the messenger RNA (mRNA) which encodes a message to initiate translation, to incorporate the specific amino acid into th
Define Classification of Lipids? Chemically, lipids are the organic molecules poor in oxygen content, soluble in organic solvents but insoluble in water. They are classified as
Give a short explanation of what UPS really means and why it is so important to health practitioners and consumers/patients.
What type of athlete would have a above average reaction time please explain.
Q. What are some antibiotics used against fungi? The systemic or topical azoles (like fluconazole, itraconazole and others), amphotericin B, the echinocandins (caspofungin, mic
How are the male gametophytes and the male gametes formed in angiosperms? In the anthers of every stamen there are pollen sacs. Within the pollen sacs there are microspore moth
how conjugation takes place in paramecium
Determine the Effect of Iodine Deficiency? Iodine deficiency affects all populations at all stages of life, from the intrauterine stage to old age. However, pregnant women, lac
Raised blood pressure is a major risk factor for cardiovascular disease. The higher the blood pressure, the higher the risk of stroke, coronary heart disease, kidney disease, heart
Infectious canine hepatitis The disease is caused by type-1 canine Adenovirus in the family Adenoviridae. The disease appears in pups as peracute, acute, and mild. The clinica
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