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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Show overall management of diabetes? Diabetes as we all know is a disease with ever increasing prevalence in' our country. It can be described as a disease characterized by
Q. Show Takhtajans System of Classification? Another important phytogenetic system was proposed by Takhtajan, the famous Russian Palaeobotanist working at Komarov Botanical Ins
Explain the Bioavailability of Vitamin D? The nutritional availability of vitamin D is less significant because it can be endogenously produced and retained for long periods by
Q. Do all mammals have a placenta? Mammals of the monotreme group (platypus, echidnas) are oviparous egg- laying and they don't have a placenta. Mammals of the marsupial group
how vagina is inserted in cock
how conjugation takes place in paramecium
The three main forms of fat found in food are glycerides (principally triacylglycerol [triglyceride], the form in which fat is stored for fuel), the phospholipids, and the sterols
Define Some Caution for Inoculating Loops and Needles? 1. Heat the inoculating wire till it becomes red hot. 2. Never use hot inoculating wire for culturing otherwise cells
What is Composition? Blood consists of red blood cells, white blood cells, and platelets, suspended in a straw-colored liquid called plasma. If whole blood is centrifuged, abou
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