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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Define Aortic regurgitation? Aortic regurgitation is a common valvular disease that may present chronically or acutely. Normally the integrity of valve closure depends upon
Oogenesis Oogenesis is the formation of ovum from oogonial cells that are formed in the ovary from primordial germ cells. And as in spermatogenesis it involves meiosis to pro
Explain the Minimum Requirement of nutritional needs? Minimum nutrient requirement is defined as the 'lowest amount of the nutrient ji-om the diet that will prevent clinically
note about water microbiology
Why would a tongue not detect mild sweetness after eating foods with high sweetness? This happens due to of the "desensitization" of sensory receptors on the sensory cells of y
Name an artificial fertiliser or fertilisers which farmers can use to increase the supply of nitrate, phosphate and potassium to their crops. NPK compound fertiliser havin
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P r o d u c t Quality Assurance Meat products should be manufactured under strict hygiene and sanitary conditions to avoid public health risks and improve aesthetic appea
Q. What is the essential morphology of a protozoan cell? Protozoans are eukaryotic cells so they have structures and organelles common to this kind of cell endoplasmic reticula
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