Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Striped pattern of the striated muscle cells formed, Q. How is the striped ...

Q. How is the striped pattern of the striated muscle cells formed? The functional units of the muscle fibers are the sarcomeres within the sarcomeres blocks of myosin and actin

How implant design and surface affect osseointegration, Q. How Implant desi...

Q. How Implant design and surface affect osseointegration? Implant design Most conductive design for osseointegration is root form. They can be threaded, hydroxyapatite co

Explain why goblet cells are non-functional, If for some reason our goblet ...

If for some reason our goblet cells are non-functional, this will adversely affect: 1. Production of somatostatin 2. Secretion of sebum from the sebaceous glands 3. Matura

What is diagnosis of heart disease in the newborn, What is Diagnosis of Hea...

What is Diagnosis of Heart Disease in the Newborn ? Clinical diagnosis of heart disease in the newborn can be quite challenging. Manifestations of potentially life threatening

Explain some ethological activities, Explain Some Ethological Activities ...

Explain Some Ethological Activities Comparative studies of behaviour or activities are also used in systematic for identification of organisms. The comparative study of the beh

What are the vitamins which make up the b complex, Q. What are the vitamins...

Q. What are the vitamins which make up the B complex? Which problems does the lack of these vitamins cause? Vitamins of the B complex are: vitamin B1 or thiamin, riboflavin, or

Define vitamins and minerals requirement for cancer patients, Define Vitami...

Define Vitamins, Minerals and Phytochemicals requirement for cancer patients? Several vitamins particularly those of the B-group are essential to promote adequate metabolism of

Which are the specialized conductive tissues of the plants, Which are the s...

Which are the specialized conductive tissues of the plants? The vascular tissues of the plants are the xylem and the phloem. Xylem is the plant tissue that forms the vessels t

Endoscopy, E n d o scopy (Greek: endon, within; skopion, to loo...

E n d o scopy (Greek: endon, within; skopion, to look at) Endoscope, a tubular instrument, is used to examine interior of body cavity or a hollow organ.  Besides e

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd