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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Invertebrates - Osmotic and Ionic Regulation Regulation of water and ions in invertebrates is done by neuroendocrine mechanism. It operates at the level of Malpighian tubules
Explain Pro-Oxidants Transition metals, particularly those possessing two or more valency states and a suitable oxidation - reduction potential between them (e.g., cobalt, cop
Preference Share Capital or Quasi-Equity It is also called quasi-equity because it combines features of equity and those of debt. It is preference because it is preferred to
Q. Treatment of diabetic ketoacidosis? The components of treatment of NKHDC are: 1) control of water loss. 2) control of sugar using insulin. 3) adjust electrolytes li
how do i prove in a diagram that water is needed for photosynthesis
What is the difference between self pollination and cross pollination? Which of these two modes of pollination contributes more to the plant diversity? Self pollination happens
Q. Drugs and Insulin used in diabetes? When diet, exercise or even weight reduction do not improve the diabetic symptoms and blood sugar levels, the uses of hypoglycemia drugs
WHAT ARE STAKEHOLDERS?
Determine the Principle of neuropsychological assessment The neuropsychological assessment of school age of children and adolescents is not primarily a technological enterpris
What (a) internal, (b) external events contribute to gain of heat in the body? a) Respiration in the tissues, particularly in the brain and active muscles, is the major in
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