Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Why the vitamin niacin is a necessary component of nad, The vitamin niacin ...

The vitamin niacin is a necessary component of NAD. Niacin can be consumed in food or manufactured in the body from tryptophan, an amino acid. How would a person's ability to break

Explain briefly protein hydrolysates, Explain Protein hydrolysates It i...

Explain Protein hydrolysates It is valued for their superior nutritional qualities, including increased bioavailability and reduced antigenecity. Several enzymatic modification

What are cotyledons, What are cotyledons? Cotyledons or else seed leave...

What are cotyledons? Cotyledons or else seed leaves are structures formed by the embryo of angiosperms to absorb nutrients from the endosperm and to store and transfer these nu

Filaments of a sarcomere in a skeletal muscle is decreasing, When the overl...

When the overlap between the thin and thick filaments of a sarcomere in a skeletal muscle is decreasing, the A. total length of the I band minus the length of the H zone is dec

How have brain-imaging methods such as pet and mri, How have brain-imaging ...

How have brain-imaging methods such as PET and MRI affected neuroscience research and clinical care? Techniques which capture pictures of the living human brain at work have be

Can you illustrate compensation point, Q. What is compensation point? What ...

Q. What is compensation point? What is the inference of the compensation point for the plant growth? The photic compensation point is the photic energy intensity under which ae

Explain the difference among dna primase and dna polymerase, Which of the f...

Which of the following is not a difference among DNA primase and DNA polymerase? A. DNA primase can initiate replication of DNA de novo whereas DNA polymerase needs a short oli

Which kind of polarity do water-soluble have, Which kind of polarity do wat...

Which kind of polarity do water-soluble and fat-soluble substances respectively have? Water-soluble substances are polar molecules, i.e., they have electrically charged areas.

Harshey-chase experiement, What is the Harshey-chase experiement .explain i...

What is the Harshey-chase experiement .explain in detail

Values of dpd for plant cells under hypertonic media, Q. What are the value...

Q. What are the values of DPD for plant cells under hypertonic, hypotonic and isotonic media? In plant cells under hypertonic medium there is loss of water for the external, SF

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd