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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Results : In the present day, surgical mortality with or without CABG is repture to be 5-7 per cent. The late survival is 85 per cent, 75 per cent and 65 per cent at 1, 3 and 5
Q. List some points to keep in mind while counselling? 1. Judgment Do not be judgemental. Be a patient listener. Assess and make decision. 2. Patience and Acceptance
Why Chordates are Schizocoelic, while other deuterostomes are enterocoelic?
Q. Do the phosphate and the pentose groups give heterogeneity or homogeneity to the nucleic acid chains? What about the nitrogen- containing groups? Supported by that, which of tho
Q. Write the definition of counselling? Definition of counselling "A process of helping or enabling a person / persons to solve certain interpersonal, emotional problems and
Define Clinical Features and Medical Complications of Anorexia Nervosa? Anorexia nervosa, as we have learnt above, is a disorder characterized by deliberate weight loss, induce
Gene Project
ELECTROCONVULSIVE THERAPY: 1) ConceptlMeaning of Electroconvulsive Therapy (ECT) Electroconvulsive Therapy (ECT) is a painless form of electric therapy. It is called as ph
Precautions It is a common observation that BP recordings are variable when taken by different individuals. To decrease this variability it is important that certain precautio
Define regulation of L-PK enzymes by starvation & refeeding? L-PK catalyzes the conversion of phosphoenolpyruvate to pyruvate and plays an important role in directing pyruvate
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