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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
In hormonal terms why does menses occur? Menses is the endometrial monthly desquamation that happens as the estrogen and progesterone levels fall after the regression of the co
what is evolution
Management of Canal Impediment a) Impediments are iatrogenic mishaps resulting from vigorous instrument short of the b) Appropriate working length and failure to confirm api
What is hydrogen bonds? Hydrogen Bonds : Hydrogen bonds form between polar molecules, such as water. Each water molecule has two positively charged hydrogen poles, and one ne
How dissimilar are the endosperm of gymnosperms and the endosperm of angiosperms? In the gymnosperms the endosperm is haploid (n), it is called as primary endosperm. In the ang
DNA replication in eukaryotes is much more complex than in prokaryotes while there are various same aspects. The Eukaryotic cells can only initiate DNA replication at a particular
Which of the following activators is correctly paired with the glycolytic or gluconeogenic enzyme that is activates? -AMP: Fructose 1,6 Bis -Citrate: Fructose 1,6 Bis -Fru
The internal composition of the cell is maintained because the plasma membrane is selectively permeable to small molecules. A lot of biological molecules are unable to distribute b
WHAT IS A CELL
Explain in detail about the optic nerve The optic nerve contains more than one million axons that initiate in the ganglion cell layer of the retina. This structure originatcs a
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