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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is the structural depiction of a carboxyl group? Carboxyl groups have a carbon attached to one hydroxyl group by a simple bond and one oxygen by a double bond. The othe
Explain about the Connecting models and data? Perhaps the most dramatic change in quantitative environmental biology in recent years has been the burgeoning progress in the dir
Medical Management Trealment of choice is cephalosporin which is given interavenously, alternatively can be given. In case of increased intra cranial pressure mannitol and o
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Explain the importance of understanding the relationship between structure and function?
Q. Enlist the various modalities available for sterility assurance? Various modalities available for sterility assurance are a) Prevention of overloading b) Chemical indi
There is no Interphase preceding second meiotic division. There is a brief intervening period called Interkinesis During this period there may be synthesis of some reserve fo
which organism are called primitive and how are they different from the so-called adavnced organisms
Explain the Decolourizing Agent - Ziehl-Neelsen Method? Acid alcohol - a mixture of 95% ethanol and 3% HCI - is used for decolourization. Before decolourization, smear is allow
what is nutrition
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