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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Lactose intolerance - Modification of carbohydrate intake? This has been covered under the section on digestion and absorption earlier. We learnt that in case of lactose
The nominalists deny the existence of Plato's "types". For them only individuals exist. Species are man-made artificial abstractions. But naturalists, the biologists who have actua
What is the genetic condition in which the heterozygous individual has different phenotype from the homozygous individual? This condition is called lack of dominance and it can
Describe the Advantages and Disadvantages of insulin pump Advantages - Insulin pumps deliver more precise amounts of insulin. This supports control over blood sugar level,
Endothecium - Anther Wall Layers The layer of cells lying immediately next to the epidermis is the endothecium, which is responsible for the dehiscence of the anther. It is us
Ground Water Ground water represents a major subterranean reservoir of fresh water. In general. it is not tapped by plants as it is too deep and does not suffer from loss due t
Coagulation - Blood Collection : Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies. There are many types of anticoagulants used
Social Basis of Primitive Life: In the previous section, we gave you a glimpse of how the primitive human beings faced the problems of day to day existence and found some so
Determine the term Anapsid ? The type of skull found in reptiles and seen today only in turtles. Behind the opening of the orbits the skull is solid and lacks temporal openings
Which of the following pairs of functional groups CANNOT react with each other by a dehydration reaction? Select one: a. Carboxyl;Hydroxyl b. Carboxyl;Sulfhydryl c. Pho
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