Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Mendel''s second law not for two or more phenotypical traits, Why is not Me...

Why is not Mendel's second law always valid for two or more phenotypical traits of an individual? The Mendel's second law, or the law of the independent assortment, is valid fo

Respiration, difference between anaerobic respiration and fermentation

difference between anaerobic respiration and fermentation

Metabolic significance of hmp pathway, Metabolic Significance of HMP Pathwa...

Metabolic Significance of HMP Pathway Having gone  through  the HMP pathway, you would have got  some idea about the significance  of this alternative oxidative  pathway for  t

Explain about human digestive system, Which organs of the body are part of ...

Which organs of the body are part of the human digestive system? The digestive system, also called as "systema digestorium", or gastrointestinal system, is composed of the dige

Oxusome, structure of oxysome

structure of oxysome

What is the effect of increase temperature, 1) What is the effect of increa...

1) What is the effect of increase temperature on a non enzyme catalyzed reaction? 2) What are the effects of increased temperature on an enzyme catalyzed reaction?

Introduction to ecosystem, The terms ecosystem was introduction by Sir Arth...

The terms ecosystem was introduction by Sir Arthur tensely in 1935. An ecosystem can be defined s " a structural and function unit of biosphere consisting of living organisms and t

What do you mean by ganglia, Q. What are ganglia? Neural ganglia or Gan...

Q. What are ganglia? Neural ganglia or Ganglia (singular ganglion) are structures located outside the central nervous system (for instance, beside the spinal column or near vis

What is st elevation, Q. What is ST Elevation? Although a stable LV ane...

Q. What is ST Elevation? Although a stable LV aneurysm may manifest ST-segment elevation in the precordial leads at rest. Some subjects with normal hearts show a degree of elev

Respiration , how many types of respiration are?define them also

how many types of respiration are?define them also

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd