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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define about the Carbohydrates? In the previous unit, we came to know that availability of glucose is the major factor in exercise performance. Hence, carbohydrate manipulation
What is Community Ecology explain their characterstics? Community Ecology: In ecological terms, a community consists of an assemblage of all of the populations living and int
During a routine annual physical, a patient was checked to determine the amount of glucose in the blood. After the assay, It was found that the glucose that the glucose concentrati
Explain What are analogies for mitochondria? Ans) Well, as we know, the mitochondria are the producers of lots of a cell's energy and the nucleolus makes ribosome's.
What is Computerised tomography Computerised tomography (CT, but also known as computerised axial tomography, or CAT) provides structural images. To generate brain scans, low l
Q How different are extracellular and intracellular digestion? What is the evolutionary advantage of extracellular digestion? Intracellular digestion is that in which the break
If a spectrophotometer can read accurately within the range of 0.1-1.0 optical density units, then how can you obtain the absorbance of a stationary culture (which may have an A550
Circulatio n I n Animal Circulation is served by a fluid as blood/ lymph that circulates through the body in a regular manner, receiving & distributing materials in the wa
Dietitians and nutritionists Dietitians and nutritionists, who are associated with hospitals and clinics generally have regular work hours. At times, they may be required to wo
What is the importance of vitamin A The importance of vitamin A is undisputable. You may already be aware about the functions/role of vitamin A in our body. Vitamin A is absol
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