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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
(i) Area uses or permit exposure to radiation should be Marked' Restricted Area 'or Radiation zone etc. (ii) Avoid x-rays of chest and lower back in routine physic
Embryology in relation to taxonomy?
Explain the Treatment of iron deficiency anaemia? Oral iron is the preferred method of treatment of IDA. The dosage is decided depending on the severity of the condition. Gener
Which of the following could potentially be a free radical? Answer oxygen (atomic number 8) helium (atomic number 2) neon (atomic number 10)
Mixture of gasoline and air is used as fuel in internal combustion engine. It is highly compressed and then ignited. 1. The process involves four strokes : 2. The f
What is Arsenite inhibitor Arsenite inhibits synthesis of ATP, in the conversion of 1,3-bisphosphoglycerate to 3- phosphoglycerate, by causing uncoupling of oxidation and
Ivory was much in demand for production of a variety of jewellery and artifacts in the past. Reptile leather from crocodiles, lizards and snakes has quite a high demand for manufac
what are the excretory organs of birds
Bilateral - Metazoa Bilaterally symmetrical animals have the major axis running from head (anterior) to tail (posterior). They have a ventral (lower) and dorsal (upper) surfac
The PCR (polymerase chain reaction) is an extremely simple as immensely powerful methods. It permits enormous amplification of any particular sequence of DNA
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