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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are holandric genes? Holandric genes are genes situated in the nonhomologous region of the Y chromosome. Holandric genes condition phenotypes that emerge only in men as in
write the classification of chordata?
List of Conditions Requiring Rapid Treatment of Hypertension 1) Cardiac: • Acute aortic dissection • Acute left ventricular failure • Acute or evolving myocardial i
Administer Accurate and Appropriate Antibiotics: Appropriate antibiotics to which organism isolated from sputum or bonchoscopic aspirate, is sensitive are administered for 4-6
Excretory organ of lizard.
Fl u o r osi s Continual ingestion of small amount of fluoride through feed or water leads to fluorosis in animals. The toxic effects are based on amount of fluorine inges
Explain the Nature of fatty acids present in the triglyceride? Nature of fatty acids present in the triglyceride determines the physico-chemical properties and biological signi
C4 plants have higher net photosynthetic rate because a.they have no photorespiration b.can photosynthesise in low in
multiple choice question
MICROBODIES Single unit membrane bound small spherical structures. Microbodies are structurally related to lysosome. On the basis of functions microbodies are of four typ
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