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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain about the Smoking - methods of food processing? Smoking was known as a method of food preservation at an early date. Foods are exposed to smokes by burning some special
what the importance of phylla protozoa
Q What are some examples of tissues and organs where mitosis is more frequent, less frequent or practically absent? Generally in vertebrates mitosis is more frequent in tissues
If you have a control group for your experience, which of the following statements is true? A. The control group should be identical to each test group with the exception of one va
What is gene? Gene is a sequence of DNA nucleotides that codifies the production of a protein.
Transcription continues until a termination series is reached. The most common termination signal is a GC-rich region which is a palindrome, followed by an AT-rich sequen
B o vi n e spongiform encephalopathy (BSE) Bovine spongiform encephalopathy (BSE) is a transmissible, neurodegenerative, fatal brain disease of cattle characterized by post
The following series of nucleotide bases forms part of a DNA molecule: T A C T T A T G A C A C A G G A G G A C T a) Convert this string of bases that would be found on the corresp
When we watch animals in the wild, most often we see them foraging for food. The foraging behaviour of animals has been a focus of behavioural studies for many decades. Natura
Define the Starch gel electrophoresis? Starch gel has the advantage of fine texture and absence of protein adsorption with a molecular sieving effect retarding proteins of larg
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