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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Parasite and host during incubation period Incubation period is the second stage in the process of a disease. It is the time between the day of infection to the appearanc
distribution of biodiversity
Which of the processes results in the most ATP production within a cell?
Explain the Copper Toxicity in Human? Acute copper toxicity in humans is rare and occurs due to inadvertent consumption of copper salts. Symptoms include vomiting, diarrhoea, h
Osmoregulatory Animal An osmoregulatory animal is generally in an osmotic steady state even though there may be hourly and daily variations in osmotic balance. The concentrati
Programmes directed towards the common crop plants These programmes can be directed towards incorporating the following traits among common crop plants. Non-photo
Q. Role of Carbohydrates in dyslipidemia? It is important to know about these carbohydrates, as they all differ in their digestive properties. The rate of absorption is variabl
Name the proteins used in oteoblast Some of these proteins include fibronectin, thrombospondin, osteopontin and osteoadherin (most of them have RGD cell binding peptide for bin
what is the origin, uses, morphology, active constituents and market perparations ?
Threshold Value of Different Larval Tissues Threshold value of dissimilar larval tissues for the thyroid hormone concentration One of the main problems of metamorphosis is
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