Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What is light reaction, What is light reaction? Light reactions are rea...

What is light reaction? Light reactions are reactions, which are initiated by light (also photo-induced reactions). Usually, light energy is change into chemical energy.

Which are the brain regions associated with memory, Which are the brain reg...

Which are the brain regions associated with memory? According to researchers some of the main regions of the nervous system associated with the memory phenomenon are the hippoc

What is mitral valve and coronary arteries, Q. What is Mitral Valve and Cor...

Q. What is Mitral Valve and Coronary Arteries? On the PA view, mitral valve calcification is seen just to the left of the spine, below the position of the aortic valve. The lar

What is the significance of choanocyte, What is the significance of Choanoc...

What is the significance of Choanocyte? Unique collar-shaped cells whose flagella are responsible for generating water current in the sponge. As flagella beats, food particles

Role of private sector in health care - competition, Role of Private Sector...

Role of Private Sector in Health Care - Competition When the private sector is driven by competition it tends to be more efficient. With competition, benefits like cost reduct

What is physiology and hemodynamic, What is Physiology and Hemodynamic ? ...

What is Physiology and Hemodynamic ? Normally, in the post-natal circulation the pulmonary and aortic circuits are in series so that the deoxygenated blood coming into the righ

Give name of the cells capable of making gametes, Q. What is the name of th...

Q. What is the name of the cells capable of making gametes? What is the ploidy of these gamete-forming cells? The cells that form gametes are the germ cells as opposed to the s

What is the principle of automated blood cell counters, Question 1 Discuss...

Question 1 Discuss various methods of blood collection in a laboratory. Add a note on collection of blood by using vacutainers. Make a table indicating the color of vacutainer tub

Explain the metabolic alterations during cancer, Explain the Metabolic Alte...

Explain the Metabolic Alterations during Cancer? As we have studied earlier, patients with advanced cancer experience profound anorexia, early satiety, changes in the structure

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd