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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
J o h n e ' s disease It is also known as paratuberculosis characterized by chronic enteritis and progressive weakness in dairy animals. E t iology: Mycobac
You have discovered that a single protein in two different cell lines is significantly over expressed in one of them. The proteins are identical, yet Northern blot analysis has dem
Define the Meaning of Goitrogens? Goitrogens are substances that interfere with iodide metabolism in any way that inhibits thyroid hormone synthesis. As a result, there is augm
Determine the Symptoms of Campylobacteriosis Symptoms: C. jejuni infection causes diarrhoea, which may be watery or sticky and can contain blood and faecal leukocytes (white
Define Transport Proteins in Plasma? Transport proteins, embedded in lipid membranes, make easy the import of nutrients into cells or the release of toxic products into the sur
phylogenetic consideration
Adverse Effects of Imiquimod Application site reactions (irritation, pruritus, flaking, erosion) are generally mild to moderate in intensity and resolve within 2 weeks of cessa
How is energy transferred along a food chain? The energy flux beside a food chain is always unidirectional, from the producers to the decomposers.
State in brief about respiration When we breathe in the air, our chest expands and air is taken in the lungs and when we breathe out chest returns to its resting position. This
Q. Clinical Symptoms of diabetes mellitus? In mild cases of diabetes mellitus, no symptoms may be seen. The diagnosis could be incidental during a blood or urine investigation.
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