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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
In infant botulism, viable botulinal spores are ingested and upon germination in the intestinal tract, toxin is synthesized. It is confined to infants under a year of age. High num
What are the capillaries of the vascular system? Capillaries are small blood vessels that perform exchange of substances among the blood and the body tissues. Capillaries are n
What is a centimorgan? The Centimorgan, or The recombination unit, by convention is a distance between two linked genes that corresponds to 1% of recombination frequency of the
1. In the human, normal pigmentation ( A ) is dominant to albinism ( a ). What results would be expected from the following matings, both genotypically and phenotypically? AA x
A fire hose ejects a stream of water at an angle of 38.8 ° above the horizontal. The water leaves the nozzle with a speed of 26.6 m/s. Assuming that the water behaves like a projec
Why and how does the food get spoiled? There are several causes of food spoilage. These include: Growth of Microorganisms, such as bacteria, molds and yeasts, which can
Consider 100 ml of 10% solute and 10 ml of 50& solute. Which has the greatest concentration of solute? What is the concentration of solute? Which has a greater quantity of solute?
Calculation of Maximum Crop Yield Percent It was pointed out previously that 1 Baule of any growth factor is equal in effect on growth to the effect of 1 Baule of any other fa
explain in brief about phyletic lineage?
Cell movements are movements performed by cell structures, like the movements of cilia and flagella, the pseudopod movements (in amoeba, macrophages, etc.), the cyclosis of the cyt
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