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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Reality Theory Developed in the 1960s by Willian glasser, a psychiatrist, reality therapists view human nature in terms of behaviour. They believe that human behaviour i
difference between holophytic nutrition and holozoic nutrition.
Results: The risk of isolated aortic valve replacement is 3-4 per cent. The long term survival for 5, 10 and 15years is 75 per cent, 60 per cent and 40 per cent respectively. The
Explain about Thermal Destruction of Enzymes during Sterilization? Before we move on to canning, we need to emphasize here that like thermal destruction of microorganisms, ther
Results: Largely depends on the LV function. In most centres surgical mortality is 3 to 4 percent. For patients with severe LV dysfunction the immediate and late results of sur
Ethylene - Plant Hormones Amongst hormones in both plant and animal kingdoms, ethylene, a gaseous hydrocarbon, is unique. Despite its chemical simplicity, it is a potent growt
#what is LH#
Disposal : You must always sterilize cultures and contaminated instruments and glassware before disposing them of or before washing-up. Usually, you can do this by autoclaving or
What are the results of Atrial Septal Defect ? Mortality for ASD closure has approached 0 per cent in most cardiac surgery centers. Late survival of patients who has ASD closu
regenerstion in invertebrates
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