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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define the Goals of treatment of Obesity? As said in the beginning, the goal of treatment of obesity today has shifted from mere 'weight loss' to 'weight management'. Each weig
Determine Energy Requirements for Athletes? You are familiar with the fact that body needs' energy to maintain body temperature, to meet metabolic demands, maintain electrolyte
What is Quantitative Techniques? Microbial quality of substances like food, milk, air, water, soil etc. can be assessed both qualitatively and quantitatively. Quantitative enum
write about asexual reproduction in mucor?
Explain the Malthus' Theory about population growth? Darwin's Theory was also based on Thomas Malthus' writings on population growth, in which he explained that populations gro
Protozoans have endoskeletons formed from microtubular cytoskeleton of the exoskeletons or cytoplasm secreted as shells or tests. Some of these single-cell organisms are capable of
Into which subphyla are the protochordates divided? What are some representatives of each protochordate subphylum? Urochordates (or tunicates) and cephalochordates are the two
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Removal of a patient's peripheral intravenous catheter resulted in brief bleeding and the loss of a small amount of blood. Which of the following processes occurred during the form
After Abutment Connection The patient may complain of pain during screw tightening. Any clinical evidence should be looked into. It is important not to overtighten and overtorq
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