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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define about the Types of indicators? The various types of indicators used are: (i) Acid-base indicators (ii) Redox indicators (iii) Metal ion indicators (iv) Preci
Alternative polyadenylation sites Exact pre-mRNAs hold more than single group of signal sequences for 3' end polyadenylation and cleavage. In some cases, the area of t
Is the stage when an insect larva is within a cocoon a stage of total biological inactivity? The period when the larva is inside its cocoon is a time of intense biological acti
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Q. What is the difference between type II diabetes mellitus and type I diabetes mellitus? Type I diabetes also known as insulin-dependent diabetes (as this name is not adequate
Pre-procedural mouth rinse Patients' use of a an anti-microbial mouthwash of 0.12% chlorhexidine gluconate solution for 30 seconds prior to intra-oral procedures will reduce th
how do respiration take place in animal????
Why after the passage of animals from the aquatic to the terrestrial habitat does the abandonment of the ammoniotelic excretion occur? Ammonia is a highly toxic molecule if not
How to reduce the stresses on the implant i) There is need to reduce the stresses on the implant - ii) Frequent recall for maintenance or hygiene appointments. iii) Gingi
ORGANIZER THEORY - It was propounded by spemann (1901). He was given Nobel prize of 1935 for this wrok. He studied the embryonic development of frog, newt & other amph
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