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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Counselling for day to day Management? As we know that diabetes is a chronic disease, a major part of its control requires counselling the patient and his family for day to
GASTRO-INTESTINA L MUCOSA - It develops from the endoderm of the embryo. Inner most layer of the wall of the alimentary canal is called mucosa. Certain cells of the mucosa
Segmentation in Regenerating Annelids Several worms as they grow continue to add new segments at the posterior end. In these, segmentation takes place in a growth zone located
Explain about the Toxicity - fat soluble Vitamin? Because vitamin A is fat-soluble and can be stored, primarily in the liver, routine consumption of large amounts of vitamin A
How much ampicillin (sodium sal, mw=371.40) would you dissolve in 400 mL of water to make 80 mg/ml solution of ampicillin?
Staphylococci exist in air, dust, sewage, water, milk and food or on food equipment, environmental surfaces, humans and animals. Humans and animals are the primary reservoirs. Stap
Stress - Responses of Plants to Stress Let us recall what happens within the natural communities occupying the same habitat. The relative location of two plants may place them
charecterstic features of phylum protozoa.
Conjugated Proteins Conjugated proteins are composed of easy proteins combined with a non- proteinous substance. The non-proteinous substance is known as prosthetic group o
Sporadic exertional rhabdomyolysis (azoturia, tying up in horses) Azoturia is a metabolic condition of horses that is characterized by reluctance to move and poor performance.
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