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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Points to Keep in Mind while feeding a preschooler? 1. Do not force-feed the child. 2. Children naturally eat a variety of foods. Their sensory-specific satiety ensur
In his experiments, Mendel noted that when two traits are involved in a genetic cross, they are inherited independently of each other. The reason for this is that A. genes on the s
CEL L BODY (= CYTON OR SOMA) OR PERIKARYON - It is nucleated & broader part of neuron. It may be up to 13.5 mm in diameter and may be irregular, spherical, oval, rounded
Non-recurrent Type As mentioned earlier it refers to embryo development in reduced embryo sacs. The mechanisms identified for such cases are: Reduced Pseudogamy : The
Reproduction
simplified carbon cycle
CELL WALL Discovered by Robert Hooke , 1665 when he saw dead empty cork cells. Bonne r - studied the chemical nature of cell wall. The cell wall is a rigid & prote
When a wound occurs in humans, platelets in the blood activate a substance that starts clotting process. The substance which starts clotting is: a) Adenosine (pron: ah-den-ah-s
The tertiary protein structure is a spatial conformation additional to the secondary structure in which the alpha helix or the beta-sheet folds up itself. The forces that stay the
In this section we are to discuss the means by which populations get separated or isolated from each other, first gain the status of'Sub-species and finally evolve mechanisms which
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