Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Hematology case study , would i be able to get help on these case study que...

would i be able to get help on these case study questions

What are the illustrations of the criminal law, What are the illustrations ...

What are the illustrations of the criminal law? Illustrations of crimes' law: Illustrations of crimes are theft, reckless or murder behaviour. The major aim of illegal

Terminal process of diseased stage, Terminal process of diseased stage ...

Terminal process of diseased stage This is the last stage in the process of disease. In this stage, the disease process is halted either temporarily or permanently. There are t

Explain changes in mental development of infants, Explain Changes in Mental...

Explain Changes in Mental Development of Infants? There is evidently an increase in the brain size. There is a rapid increase in the number of brain cells in the first 5-6 mont

ACTINOMYCETES, WHAT ARE THE DIFFERENT CULTURE MEDIAS USED FOR ISOLATION OF ...

WHAT ARE THE DIFFERENT CULTURE MEDIAS USED FOR ISOLATION OF ACTINOMYCETES?

De vega annuloplasty, De Vega Annuloplasty :  Classic De Vega annulop...

De Vega Annuloplasty :  Classic De Vega annuloplasty is done using 2'0' double armed polyester suture with pledgets on either end. It starts with one of the needles passed fr

Natural and artificial ecosystem, Natural and Artificial Ecosystem i) N...

Natural and Artificial Ecosystem i) Natural ecosystems are those which are mostly free from human disturbances, such as tropical forests, grasslands, oceans, lakes and deserts.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd