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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Have you ever said, ‘I cannot cope up with this?' Or have you heard any one who is sick in your family saying, ‘I cannot cope with my sickness' or ‘I can not take care of this sick
Determine the list of Sensory Organs There are four special sensory organs in our body. They are: 1) Eyes 2) Ears 3) Nose and 4) Skin
What is Stem cell therapy Most defects / diseases treated by stem cell therapy are not genetic in origin so the therapy will have no impact on gene pool in terms of change in f
Determine the main four events of bone remodeling Quiescence refers to the resting state of the bone surface. This includes all of the bone surfaces. Activation is the recr
Access through the crown Advantages : -Allow for better isolation with the rubber dam -Better esthetic anf functioning. -Good coronal seal between visits Limitations : -Proc
Rickets Rickets is a complex mineral deficiency disease of young, growing animals. It is characterized by defective mineralization of growing bones, persistence of hypertrophic
How old is the universe? From the analysis of data collected by the Hubble telescope the age of the universe is estimated to be about 12 billion years.
How was it proved in the case of Amoeba that the key to the life of a cell is Nucleus?
Cardiac Output - Circulation The cardiac output can be determined in a number of ways. The most common method is the Fick's principle. A. Fick a German physiologist in 1870 de
Explain the Basal Metabolic Rate? The Basal Metabolic Rate (BMR) gradually decreases after the attainment of maturity due to a decrease in muscle mass tissues and physical acti
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