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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
A microbiology student isolated a hypothetical bacterial cell that seems to quadruple every generation. Knowing that binary fission is an exponential function, derive a mathematica
Characteristics Define Cancer - Hyperplasia Hyperplasia is the extreme proliferation of cells which can be observed in normal as well as cancerous tissues. In normal tissue, a
Problem: Explain the procedure for import of cosmetics in US. - Briefly explain the procedure for import of cosmetics in US What are the major points to be considered whe
Fatty Acid Metabolism The body has a limited supply of glucose associative to the energy stored as fat. There are 3 sources of fatty acids for energy metabolism within animals;
hows does insulin from the pancreas reach the liver?
#Predict: In order to be able to energize a transport protein by phosphorylation (by addition of a phosphate group from ATP), the portion of the protein to which the phosphate grou
Q. Illustrate Physiological Evidence ? Physiological and biochemical evidence is providing data of increasing importance to plant systematic. Recently, it has become apparent t
Methods of Filter Feeding More elaborate methods of filter feeding are seen in tube dwelling polychaetes which use tentacles to entangle the food particles. Figure shows some
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Ultrasonic removal of Gutta percha: -the piezoelestic ultrasonic system. - As the instruments is energized; producing heat to themosoften gutta-percha. - Float gutta-perc
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