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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Nutrient Availability You have seen that cation exchange plays an important part in nutrient availability to plants. Cation exchange functions in two ways as nutrients are rele
Q. What are the conditions favouring the outbreak of Staphylococcal food poisoning? • Botulism is caused by the bacteria Clostridium botulinum. • All low acid foods can s
Q. Carbohydrates requirement during diabetes? Carbohydrates: These to a large extent depend on the food habits. Complex carbohydrates with more fibre are recommended to simple
How does the pancreatic juice resume the digestion of carbohydrates? What is the involved enzyme? Carbohydrate digestion starts with the action of the salivary amylase (ptyalin
Pulmonary stenosis is a relatively common congenital heart defect. Usually these children with mild to moderate pulmonary stenosis survive into childhood. Since bicuspid pulmonary
VERTEBR A T A It is divided into two parts - 1. Agnetha 2. Gnetha S.No. C haracter A gnetha G
Basic characters of vertebrate classes
Q. What is a prion? The prion is an infectious (transmissible) protein able to replicate by transforming other proteins into a copy of the prion. The mechanism of the copying i
Comparing toxicity and the requirement for dilution in water how different are the ureotelic and the uricotelic excretions? What are some examples of animals that present these res
Q. For non-insulin users consumption of Alcohol? 1. Substitute for fat calories. A drink providing 100 calories should be substituted by 2 fat exchanges. . 2. Avoid if your
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