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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Describe the Phylum Arthropoda and Metamorphosis? More than one million different species of arthropods are estimated to exist on the earth today-more than all other animal spe
Which is the biological molecule that contains the genetic information that is transmitted hereditarily and controls the cellular functioning? The hereditary molecule that cont
By what means can malarial parasites be transmitted from an infected person to a healthy person? The malaria parasite is transferred when a healthy person is bitten by a m
Explain An important strategy of group meetings An important strategy could be to conduct small group meetings for behavioural change encouraging full participation. The group
Does every gene mutation cause alteration in the protein the gene normally codifies? Not each gene mutation causes alteration in the composition of the protein the gene codifie
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A restriction map of plasmid and a human gene of identified sequence is demonstrated below. Your job is to clone the human liver cDNA for that gene than the start codon is closer t
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Hydrocolloids are extremely versatile and are used for many other purposes including: (a) Production of pseudoplasticity (i.e. fluidity under shear) at high temperatures to ea
Determine the Types of Exercises Exercises are generally grouped into three types: - Flexibility exercises such as stretching improves the range of motion of muscles and jo
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