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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Preparation on the Previous Day of Operation Remove all the jewellery, nail polish, cut nails. The patient's body is cleaned - shaving from chin to toe, inclu
R E GENER A TIO N - Natural ability of living being to replace worn out parts, to repair damaged parts, to regrow cost off organs & to reform entire body from a small piec
Q. How is the respiratory system in aquatic molluscs characterized? What adaptive respiratory structure do terrestrial molluscs present? In terrestrial molluscs the rich vascul
Three ways in which food might become contaminated by harmful bacteria. Food may become contaminated:- (i) from the unwashed hands of a food-handler, (ii) by house- flies
Sporadic exertional rhabdomyolysis (azoturia, tying up in horses) Azoturia is a metabolic condition of horses that is characterized by reluctance to move and poor performance.
Explain the Ebb or Shock Period - Dietary Management for Burns? During the initial bums after injury, the focus is on counteracting the stress induced neurohormonal and physiol
Specific Guidelines Physician's orders Each order should have the date and time it was written. Some way of indicating which orders have been carried out is nece
Define Interferons - Humoral Mechanisms Interferons are soluble proteins secreted naturally when cells become infected by foreign bodies. Infected lymphocytes release interfero
(a) What are the intermediate products of anaerobic respiration in an active muscle? (b) Which of them is associated with oxygen debt? (c) In what way is th
Air Microbes
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