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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Photoreceptor - Phototropism The molecular nature of the photoreceptor is unsolved. Initially in the 1930s, some form of carotenoid was thought to be the phototropic receptor.
Describe Infectious Agents ? Recently there has been increasing interest in the relation between chronic low grade systemic inflammation and mortality of coronary artery diseas
What is the best microscope to get a detailed view of the parts inside of a preserved plant cell
Do all vascular plants develop annual rings? Vascular plants are those, which have phloem and xylem structures within them to transport water and nutrients around the plant. Mo
Which one of the following statements about morula in humans is correct? 1. It has almost equal quantity of cytoplasm as an uncleaved zygote but much more DNA 2. It has far l
Define Patient Education for preventive strategies for food allergy? Patient education, therefore, is critical and should include: Allergen identification (e.g., how to
Define Feeding and Nutritional Management for patient of alzheimer's disease? Keeping the clinical manifestations of Alzheimer's disease in mind, treatment involves personaliz
Blood Plasma - Circulation Centrifugation of blood results in its separation into two portions - a packed mass of cells which constitute around 45 per cent of the volume of t
Explain Viscosity of proteins Viscosity reflects resistance to flow. The main single factor influencing the viscosity behavior of protein fluids is the apparent diame
Explain Interactions of Macrominerals With Other Nutrients? Various nutrients interact with minerals thereby affecting their bioavailability. These interactions occur at differ
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