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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Morphology in relation to taxonomy? Morphology is the basic tool of taxonomy, because identification is primarily based on the characters of the plant. The morphological cha
Biotechnology: Biotechnology is, perhaps. best defined as the industrial utilisation of biological systems or processes. In sense, therefore, biote(biology ha existed fo
Vitamin B 1 (Thiamine hydrochloride) a) Thiamine hydrochloride is a white, crystalline powder, slightly hygroscopic, of an odour resembling yeast and of bitter to taste. b)
Determine the fuction of Monomeric enzymes Monomeric enzymes include, pepsin, like the pancreatic serine proteases, plays a role in the digestion of proteins eaten by mammals.
CARBOHYDRATE METABOLISM Carbohydrates are broken down into monosaccharides which are absorbed into the blood stream. In the liver and muscles, most of the glucose is
Q. Important aspect of dietary management during hypertension? Most important aspect of dietary management i.e. the intake of minerals and electrolytes, which are closely assoc
Q. What are the few examples of the energetic function of organic molecules? Since they are complex molecules, organic molecules store large amount of energy, presenting many c
What is Cerebral Hemorrhage Cerebral hemorrhage is a massive bleeding into the substance of the brain. The most frequent cause is high blood pressure, or hypertension. Other ca
Question 1 List various liver function tests. Discuss the importance of each test. Add a note on standardization of various liver function tests Question 2 Discuss the follow
MULTIPLE ALLELES (i) More than two alternative forms (alleles) of a gene in a population occupying the same locus on a chromosome or its homologue are known as multiple
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