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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What is viscosity? Viscosity is resistance to flow of a liquid. It is a measure of the resistance of a fluid to deformation under shear stress. It is commonly perceived as "
Define Quantitative Procedures in Carbohydrates? You have learnt how to identify some individual monosaccharides, disaccharides and polysaccharides. However, if we were asked t
Q. Explain about precautionary principle? The ethical values of biodiversity highlights the intrinsic value of biodiversity for its own sake and it is independent of the varied
how many atoms are H2SO4 compound
Temperature - Aquatic Habitats The water temperature changes less rapidly than the temperature of air because water has a considerably higher specific heat than air, that is l
Ribonucleases and deoxyribonucleases These enzymes are responsible for the degradation of dietary nucleic acids. Ribonucleases and deoxyribonucleases secreted by the
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Vivipary - Development Biology In flowering plants the seed or fruit generally is dispersed and germination occurs when the conditions are congenial for growth. However, in so
Similarities in body parts often indicate shared ancestry. Which of the following is true? Structures used for different purposes in different groups can be controlled by the same
Describe Respiratory System? The respiratory system is responsible for the transport of oxygen and carbon dioxide from outside the body to and from the blood. This process is c
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