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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Factors that affects Oxygen Dissociation Curves Oxygen dissociation curve for a sample of blood is affected by several factors. The most important of them are: Te
Explain about the Renal and Urogenital System? Various studies have shown a decrease in the creatinine and insulin clearance. Due to the changes in glomerular structure of the
Vitamin K (Menadione - Vitamin K3, oil soluble) Menadione is a yellow, lustrous, crystalline powder. It is easily soluble in benzene, soluble in fats and fatty oils. It is spa
Explain the Objectives of Congenital Heart Disease ? After reading this unit, you should be able to: 1 Comprehenced the epidemiologic significance of congenital heart disease (
Inorganic Nitrogen and Sulphur Metabolism Productivity in agriculture, forestry and other ecosystems is basically limited by the availability of nitrogen nutrients like nitrat
Q. Ecological Evidence of organisms to environment? Ecology is concerned with organisms in relationships to their environment. These relationships may be classified as biotic,
Reproduction
What is vernalisation? How is the process of vernalisation benefits to plants?
Type 1 Diabetes Mellitus occurs when the body's immune system destroys beta cells. Beta cells produce insulin, a hormone that helps to move the glucose contained in food into cells
Q. What are the Chronic complications of diabetes? • Atherosclerosis: Degeneration of walls of the arteries due to fatty plaques - deposition on arterial walls. Diabetics ar
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