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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Signify Asexual and how it is formed? Organisms which do not reproduce by recombination of genetic material contained in gametes. There is not any combination of a haploid sper
Explain the Precautions for Detection of Number of Bacteria in Milk? 1. Make a thin film of milk on the slide. 2. Do not over stain with methylene blue. If over staining ha
how to explain an example from each class of vertebrate
Which one of the following statements about morula in humans is correct? 1. It has almost equal quantity of cytoplasm as an uncleaved zygote but much more DNA 2. It has far l
Determine the Food Sources of Iodine? Please note that unlike other minerals studied so far, like selenium, the iodine concentration in foods is highly variable and also depend
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What is Defense mechanism Defense mechanism relate to the body's ways to protect itself from any infection or a disease
Explain about the Foods Effect on Drug Utilization? The following illustrations highlight the effect of food on drug utilization. Liver and green leafy vegetables can decrea
Q. Pathophysiology of mitral stenosis? Normally there is no pressure gradient between left atrium and the left ventricle during diastole. However, as the valve orifice size dec
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