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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
SURGERY FOR CORONARY ARTERY DISEASE : Stenotic coronary artery disease (CAD) is caused by the thickening and narrowing of the coronary arteries (Atherosclerosis). Initially it cau
what are the general methods for studying microbial physiology
Q. Rapport Building of Counselling process of diabetic patient? 1. Rapport Building - Introduce yourself to the counsellee. - Get the introduction of counsellee. - Sit
STRUCTURE Golgi body consists of :- (1 ) Cisternae These are plate like unbranched structure with dilated ends. One golgi body has 4-8 cisternae. Mono cisternal
Paclitaxel is a anticancer drug. It stabilizes microtubules and as a result, interferes with the normal breakdown of microtubules (you will know about microtubules in future lectur
Define effect of dietary fibre expect physiological effects? In addition to all the physiological effects mentioned above, dietary fibre may exert other effects. Acetate and pr
what are the general characters of chordates? At least 10 characters.
State about the Recti and Oblique Muscles The extra-ocular muscles are mainly involved in the maintenance of postural tonicity and performance of quick contractions. These mus
Q. Explain Therapy for diarrhoea? The therapy for diarrhoea thus consists of: 1. Determining the status of dehydration 2. Fluid management * Oral Rehydration Therapy (
Which is the vertebrate class that is considered the first entirely terrestrial? The first totally terrrestrial vertebrate class, totally independent from the aquatic habitat,
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