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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What do we mean by fixation? Fixation is the process of preserving internal and external structures of microorganisms. It leads to the killing of microbial cells and their fir
identifying characters of pennetula
Q. Meaning of Diabetes Self Management Education? You have learned that the treatment of diabetes depends upon the cooperation of the patient and also on how actively a patient
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Introduction: There are different technologies available for dealing with waste water management and excreta. Some are suitable for dealing off-site sanitation and other can b
Q. What is the cost-benefit relationship regarding sewage treatment as a strategy to fight water pollution? To treat sewage is greatly cheaper for society. The non-treated sewa
Infusion Pump As you'have learnt earlier, the amount of fluid to be administered to neonate is based on the neonate's weight and physiological status. It is recommended that
What are hormones? Hormones are chemicals secreted by the body to create changes in physical appearance, reproductive behavior, and emotions. Hormones are organic chemical m
is a wheat plant a eustele or a siphonostele or a protostele?
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