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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the Penicillium - Fungi? Classification Kingdom - Mycetae Division - Amastigomycota Class - Ascomycetes Subclass - Plectomycetidae Order - Eurotiales
mendal laws
Explain the differences between a thrombus and an embolus. Include predisposing factors, mechanism of occurrence, treatment, and methods of detection with medical imaging, if any.
Vascular Cambium The vascular cambium usually, if not always, has a dual origin within the primary tissues; from provascular strands, and from the "ground" meristem tissues be
Functional properties of food lipids Chemically, fats and oils, as you may already know by now, are a combination of glycerol and fatty acids. The glycerol molecule has three s
A student while studying the anatomy of leaves of four specimens (P - S), observed the following characters: P. Reticulate venation and no bundle sheath. Q. Parallel venati
Q. What is the structure of the adult fern within which cells undergoing meiosis can be found? In these plants meiosis takes place within structures known as sorus (plural, sor
a 6th grade student friendly definition?
What is the relationship of hydrogen ion concentration in tomato juice relative to lemon juice and to pure water? Be as quantitative as you can (i.e., use numbers to describe the m
Explain Objectives of Root-End Resection Apicoectomy a) Removal of untreated apical portion of the root, b) Enable the obturator to determine the cause of failure, c) To
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