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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Mineral biofortification of plants One sustainable agricultural approach to reducing the mineral deficiencies in livestock animals is to enrich major staple food crops (rice,
Define Surface properties of Proteins? The surface properties relates primarily to surface tension, emulsification and foaming characteristics of proteins, which are discussed
FUNCTIONS OF CELL WALL 1. Provides shape to plant cell rigidity to cells. 2. Functions as a barrier to entry of pathogens into the cells. 3. Provides pr
What are the three main limiting factors of photosynthesis? The three major limiting factors of photosynthesis process are light intensity, carbon dioxide concentration and tem
State four steps to be followed to test for starch in a leaf
what is the deference between sp. and spp.?
Explain about Thermal Destruction of Enzymes during Sterilization? Before we move on to canning, we need to emphasize here that like thermal destruction of microorganisms, ther
The vagus gives of one superior cervical cardiac branch and an inferior cervical cardiac branch in the neck. These branches descend into the thorax and take part in performing the
1. A completely new protein has been synthesized by a research lab. You are inadvertently exposed to it and have an allergic response. What type of antibody initiates this respons
Maintain nutrition Your responsibility as a nurse is to take care of nutrition and prevent the child from starvation. Breast feeding'should be continued during the diarrhoea e
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