Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Explain non-starch polysaccharides, NON-STARCH POLYSACCHARIDES A polysa...

NON-STARCH POLYSACCHARIDES A polysaccharide often termed as complex carbohydrate. Besides starch, a mixture of substances called non starch polysaccharide (NSP), also constitut

Products of hemp plant, PRODUCT S OF HEMP PLANT - Four drugs - bhan...

PRODUCT S OF HEMP PLANT - Four drugs - bhang, ganja, charas & marijuana obtained from Cannabis indica and C. sativa of family Moraceae . Main alkaloid is tetrah

Chordates, what are the general characters of chordates? At least 10 charac...

what are the general characters of chordates? At least 10 characters.

The human abo blood type codominant alleles a and b, The human abo blood ty...

The human abo blood type codominant alleles a and b are dominant to O. A man with blood type B and women with blood type A could potentially have children with which of the followi

Male reproductive disorders-aplasia of epidydimis, Aplasia of epidydimis ...

Aplasia of epidydimis This condition is seen in only 1 or 2 % of young bulls and is marked by absence of one (more common) or both epididymis. Bulls having a single epidydimis

Counter current flow, Counter current flow - Respiration Each filament...

Counter current flow - Respiration Each filament has an upper and a lower row of flat lamellae. The lamellae of successive filaments are in close contact. Gas exchange takes p

Define foam spray drying method, Define Foam Spray Drying Method? This...

Define Foam Spray Drying Method? This is an extension of spray drying and involves the use of gases dissolved under pressure prior to spraying. The main advantage is that the

Proteins requirement in dyslipidemia, Q. Proteins requirement in dyslipidem...

Q. Proteins requirement in dyslipidemia? Proteins: While the quantity of protein does not impose any significant impact on the serum lipoproteins, it is the quality of protein,

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd