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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Renal Glycosuria Renal glycosuria is a condition in which the glucose is excreted in the urine in spite of normal blood sugar levels. Self Monitoring of Blood Glucose (SMBG
Define methods of prevention and control of anaemia? The available methods of prevention and control of anaemia are: Supplementation Food fortification Dietary
Define the Essentiality of Carbon for the Growth of Microorganisms Carbon is needed for the skeleton of all organic molecules. Microorganisms acquire it either as inorganic car
Explain about the Dehydration or Drying? The technique of drying is the oldest method of food preservation practiced by mankind. The removal of moisture, which is actually dehy
Which are the heart chambers respectively where the entrance and the exit of blood occur? The heart chambers by which blood enters the heart are the atria. There are the right
Energy Contrary to matter. Energy neither occupies space, nor possesses mass. Hence it is defined, not on physical ,but on operational or functional basis as the force whic
Determine the Functions of Vitamin C? The vitamin C is involved in several physiological and biochemical functions in the body. Ascorbate is the biochemically active form of t
Explain about the Gelation? Gelation refers to the process where denatured molecules aggregate to form an ordered protein network. Proteins can form a well-ordered gel matrix b
Phimosis Phimosis may be congenital or acquired. The foreskin in young infants cannot be retracted, because at that gge it is adherent to the underlying glans. Wlen an attemp
What are the two big groups into which cells are classified? Cells can be divided as eukaryotic or prokaryotic. Prokaryotic cell is the cell that without a delimited nucleus
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