Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Basic principles involved ark, Q. Basic principles involved ark? The ba...

Q. Basic principles involved ark? The basic principles involved ark: The individual must be treated as such and for that careful initial history - daily living situation, a

Define briefly about vitamin B1, Vitamin B 1 (Thiamine hydrochloride) ...

Vitamin B 1 (Thiamine hydrochloride) a) Thiamine hydrochloride is a white, crystalline powder, slightly hygroscopic, of an odour resembling yeast and of bitter to taste. b)

Purposes of patient /client records, PURPOSES OF PATIENT's /CLIENT's RECO...

PURPOSES OF PATIENT's /CLIENT's RECORDS Following purposes are served by maintaining patient's/client's records: A means of communication.  A basis on which therap

Vitamins requirement during myocardial infarction, Q. Vitamins requirement ...

Q. Vitamins requirement during myocardial infarction? The requirement of vitamins and minerals is largely governed by the existing nutritional status and the clinical parameter

What are cd4 lymphocytes, What are CD4 lymphocytes? What is the relationshi...

What are CD4 lymphocytes? What is the relationship between these cells and HIV? How does HIV replicate? CD4 lymphocytes are T helper lymphocytes that there in their plasma memb

What nerve is being compressed, what major nerve do you think is being comp...

what major nerve do you think is being compressed when a person often feel pain in the posterior surface of the thigh radiating to the area behind the knee and where is the likely

Define the historical process of spatial processes, Define the Historical P...

Define the Historical Process of Spatial processes? In year 1952 Alan Turing developed his theory of the generation of pattern in systems of reaction diffusion equations, now k

The population thus contains secretors, The A and B antigens in humans may ...

The A and B antigens in humans may be found in water-soluble form in secretions, including saliva, of some individuals (Se/Se and Se/se) but not in others (se/se). The population t

How many chromosomes does a human normal haploid cell have, Q. How many chr...

Q. How many chromosomes does a human normal diploid cell have? How many chromosomes does a human normal haploid cell have? How many are the sex chromosomes within each of them?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd