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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Difference between aqueous humour and vitreous humour - Aquou s Humour Vitrou s humour 1. Occurs in aquous chamber 2. Wat
Q. Function of Glutamate? Glutamate It is the principal excitatory transmitter in the brain and is found throughout the central nervous system. Receptors Glutamate rec
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Define Practical Preparation - Qualitative Test for Monosaccharides? 1. Wear your labcoat. Make sure your hair is tied and you have no loose clothing. These can be dangerous in
Phytochrome - Floral Induction Phytochrome a pigment that exists in two different inter convertible forms was discovered by Hendricks and Borthwrick et. al. and was chemically
Regeneration - Root and Shoot Morphogenesis Higher plants have three kinds of regenerative activities; All plants possess inherent ability to regenerate lost parts. Fo
Characters of pennetula
Algae have cell wall made up of: 1. Cellulose, galactans and mannans 2. Hemicellulose, pectins and proteins 3. Pectins, cellulose and proteins 4. Cellulose, hemicellulo
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