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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define the Medical Complications of Bulimia Nervosa? The medical complications of bulimia nervosa range from fairly benign, transient symptoms, such as fatigue, bloating and co
Transcription continues until a termination series is reached. The most common termination signal is a GC-rich region which is a palindrome, followed by an AT-rich sequen
A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction
Ernst Mayr, for whom, species and species problem have been the main concern of research, points out that speciation, the multiplication of species, that is division of one parent
Amoebae- Parasitic Protozoan The amoebae of the genus Entamoeba vary in their biology. Entamoeba histolytica or the dysentery amoeba occurs as a parasite in the large intestin
C o mbination of meat products Combining meats and byproducts of different species to produce value added products results in complementation and supplementation of their qua
Q. What si Adenosine or Dipyridamole Thallium Scan? These studies are newer modalities that allow studying perfusion abnormalities to the myocardium when the patient cannot per
Q. Illustrate the Methods to evaluate osseointegration? The criteria of success in implant dentistry remains complex. The vast majority of clinical reports noting success and f
Enumerate the absolute and relative contraindications. 1. Absolute contraindications - Recent myocardial infarction - Valvular prosthesis - Severe renal disea
What is Radioactivity ? Radioactivity : Some isotopes, called radioisotopes, have unstable nuclei that release high energy particles or radiant energy, or both. An example
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