Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What carbohydrate characteristic chemical features include, Carbohydrate ch...

Carbohydrate characteristic chemical features include: Select one: a. the potential to form multiple hydrogen bonds. b. the existence of one or more asymmetric centers.

What is the cardiovascular system, What is the cardiovascular system Th...

What is the cardiovascular system The cardiovascular system, nervous system, kidneys and special sensory organ, eyes is directly affected by diabetes and leads to fatal conditi

Explain uncompetitive inhibition, Uncompetitive inhibition  In this typ...

Uncompetitive inhibition  In this type of inhibition, the inhibitor only binds with the enzyme-substrate complex making  it  inactive.  As  a  result,  the  product formation

Which are the brain regions associated with memory, Which are the brain reg...

Which are the brain regions associated with memory? According to researchers some of the main regions of the nervous system associated with the memory phenomenon are the hippoc

Trihybrid cross between two true-breeding homozygous, Which of the followin...

Which of the following statements is true regarding a trihybrid cross between two true-breeding homozygous individuals with contrasting phenotypes? Which of the following statement

Determine about the chloroplast, Determine about the Chloroplast One of...

Determine about the Chloroplast One of the most distinguishing features of all eukaryotic plant cells is that in addition to mitochondria, they contain special light harvesting

Life cycle of parasite - protozoan, Life cycle of Parasite - Protozoan ...

Life cycle of Parasite - Protozoan The life cycle of the parasite in man begins when an infected mosquito, while biting and taking a blood meal, injects sporozoites into the

Clinical trials for changing nutrient requirements with age, Define Clinica...

Define Clinical Trials for Changing nutrient requirements with age? Several clinical trials provide evidence of changing nutrient requirements with age. The metabolic demand fo

Discovery of insulin, Q. Discovery of insulin? The discovery of insulin...

Q. Discovery of insulin? The discovery of insulin has dramatically changed the lives of people having type 1 diabetes. With this wonder drug diabetics can lead a normal, enjoya

Explain procedure for capsule staining in a culture, Explain Procedure for ...

Explain Procedure for Capsule Staining in a Culture? We have learnt that Capsule staining can be undertaken either by negative staining or Anthony method. Now carry out the exe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd