Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Describe insulin resistance in non-conventional factors, Describe Insulin r...

Describe Insulin resistance in Non-conventional Factors ? Insulin resistance refers to a generalized metabolic disorder in which various tissues are resistant to normal levels of

Zoology, characteristics of class mamalias

characteristics of class mamalias

Bovine viral diarrhoea, B o v i ne viral diarrhoea Bovine viral dia...

B o v i ne viral diarrhoea Bovine viral diarrhoea (BVD) and mucosal disease (MD) are clinically dissimilar disease syndrome yet have a common viral etiology. The acute dise

What is the vector of chagas disease, Q. What is the vector of Chagas' dise...

Q. What is the vector of Chagas' disease? How is the disease transmitted? The vector of the Chagas' disease is its intermediate host, a triatomine bug. The key species is Triat

What are the causes of endodontic pain, What are the causes of endodontic p...

What are the causes of endodontic pain? Lesions in retreatment cases called post endodontic diseases. Reasons of Endo retreatment; = reasons of failure: - Signs or symptoms of

How all the exercises are active exercises, How All these exercises are act...

How All these exercises are active exercises All these exercises are active exercises that is active exercises are those type of physical activities carried out by the individ

Change of health status over time, Change of Health Status Over Time Hea...

Change of Health Status Over Time Health of a nation can be gauged from the available information on death.Disaggregated data by causes of death is more reflective of the status

Define apoptosis, Question Write a short note on the following- 1 Ph...

Question Write a short note on the following- 1 Phases of fertilization 2 Blastulation 3 Germ layer derivatives 4 Gap genes 5 Discuss any 5 potentials and limita

Bovine rotavirus diarrhoea, Bovine rotavirus diarrhoea The bovine rota...

Bovine rotavirus diarrhoea The bovine rotavirus is a RNA virus with 11 segments of double stranded RNA belonging to the genus Rotavirus in the family Reoviridae. Rotaviruses c

How age factors affect the requirement of protein, How age factors affect t...

How age factors affect the requirement of protein? Age: Protein in excess of maintenance needs is required, when a new tissue is being fornled. Certain age periods, when growth

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd