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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Complex or Undefined Media? Here, the exact composition of the medium is not known. It contains some complex components of plant and animal extracts whose exact chemica
How Campylobacteriosis is caused It is caused by Campylobacter jejuni, a Gram-negative rod. It is a microaerophillic organism, which means it has a requirement for reduced lev
Food Applications Dextran can be used in food products, as it is capable of moisture retention and inhibition of crystallization of sugar. The properties of dextran as gelling
Explain in detail about the Concomitant squint Concomitant squint is also called comitant squint. It means "I follow you", i.e., in all directions of gazes, the squinted eye fo
Q. What is the usual shape of poriferans? Sponges have bodies in the form of globes or tubular vases open in the upper extremity. They have a porous walls and internal central
Define Clinical Significance and Risk Factors for Drug-Nutrient interactions? We are already aware of the fact that poor nutritional status can impair drug metabolism and the d
SCOPE OF BIOLOGY- Present century is witnessing explosion of information in modem biology directly related to life and health. Issues like global warming, pollution, populat
Determine Calcium requirements of school children and adolescents? Calcium requirements in children and adolescents can be calculated on the basis of Calcium accretion during g
Foot examination For all diabetics, foot examination is important and necessary in all visits to identify risk factors of ulcers or injury of foot. This will help in preventing
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