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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
State the phases of soil genesis Soil genesis embodies two distinct phases. One is weathering that involves disintegration and decomposition of rocks and minerals, the other is
Which of the following serves as an actuating signal, or as part of an actuating signal, in a system with feedback? (Either positive feedback or negative feedback) A. Blood pla
How do birds reproduce? Birds, like each vertebrate, have sexual reproduction. Their embryos develop within shelled eggs having extraembryonic membranes and outside the mother'
You now want to express the gene in E coli, but unfortunately it has an intron in it that will not be processed out in bacteria, hence it must be removed. (a) Design P
What is Archaebacteria ? Archaebacteria is a relatively new grouping that was created in the light of relatively recent biochemical and electron microscopic evidence. These fin
what is example of class,genus and species of platyhelminthes?
Categories of Air Pollutants From the above list you can see that the air pollutants can be broadly classified into the following two categories: Primary Pollutants
What is the Definition of Polyphenols? Polyphenols are the compounds that contain an-ON group attached to the benzene ring. The main classes are: flavonoids, phenolic acids and
SHARPS : Cuts due to careless handling of sharps such as sectioning razors, microtome blades etc. are probably the most common cause of injury in the biology lab. The only real re
There are specific sensitive/critical periods in development during which acquisition of certain skills is substantially easier than at other times. In the animal domain there are
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