Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Explain the culture media and its types, Explain the Culture Media and Its ...

Explain the Culture Media and Its Types? A culture medium (Pl. media), we already know, is a solid or liquid preparation containing all the nutrients required by microorganisms

Explain natural classification, Natural Classification Natural classifi...

Natural Classification Natural classification is based on the natural characters of the taxa. Some consider natural classification a phylogenetic one reflecting the evolutionar

Sex, How can I fuck my girlfriend without taking out cum?

How can I fuck my girlfriend without taking out cum?

Explain the history of diet nnd, History of diet nnd or  tube feeding toler...

History of diet nnd or  tube feeding tolerance History of diet nnd or  tube feeding tolerance: The dietitian  presents  the patient's history, which may  include presence or hi

Composed of only glucose monomers, Which of the following is composed of ON...

Which of the following is composed of ONLY glucose monomers? (mark all that apply) Select one or more: a. Starch b. glycogen c. Maltose d. chitin e. Cellulose

Why is a leguminous crop rotation used in agriculture, Q. Why is a legumino...

Q. Why is a leguminous crop rotation used in agriculture? The Leguminous crop rotation and other crop rotations are used in agriculture for the reason that in these plants many

Define and compare bone modeling and remodeling, Define and compare bone mo...

Define and compare bone modeling and remodeling Bone modeling is a surface-specific activity (apposition or resorption) that produces a net change in the sie and/or shape of a

Explain the process of diagnosis of cholera, Explain the process of diagnos...

Explain the process of diagnosis of cholera Diagnosis: Cholera can be confirmed only by the isolation of the causative organism from the diarrheic stools of infected individual

Explain turnover number, Explain Turnover number Turnover number:- Th...

Explain Turnover number Turnover number:- The  number  of  molecules  of substrate  transformed per catalytic  site of the enzyme per minute,

What is biotic potential, Q. What is biotic potential? The Biotic poten...

Q. What is biotic potential? The Biotic potential is the capability of growth of a given population under hypothetical optimum conditions, i.e., in an environment without limit

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd