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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Some people believe that being a vegan is the most responsible way to get nutrients while still maintaining the balance of the ecosystem. Explain this rationale, giving solid infor
Define Tips that should be followed before an event Start? Before the event, following tips should be followed: As the glycogen stores may be used up during night, a h
Define Vitamin K-dependent carboxylation? Vitamin K acts as a cofactor in the synthesis of g-carboxyglutamic acid (Gla) from glutamic acid residues required for the normal coa
Human Genome Initiative is the collective name for number of projects begun in 1986 by Department Of Energy (DOE) to (1) create an ordered set of DNA segments from known chrom
what is the difference between neurological and neurovascular observations
description on characteristics of pisces fish
Disadvantages of Ridgemapping a) Traumatic procedure b) Time consuming procedure and needs more armamentarium c) There is an inherent risk of inaccuracy especially whil
Respiratory system of Crustacea ?
Define the Iodine Test - Carbohydrates? This test is given by polysaccharides and is not given by monosaccharides or disaccharides. Principle Iodine forms a coloured ad
Gum Tragacanth Gum tragacanth, the exudation of Astragalus species, is defined as the "dried gummy exudate" obtained from Astragalus gummifer Labillardiere. Gum tragacanth is
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