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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Dark Field Microscope In this microscope, condenser system is modified, so that only reflected and refracted light by specimen forms an image. Here, the object is bright
E n z y m e - linked immunosorbent assay (ELISA): ELISA in its var io us modifications e.g. plate ELISA, sandwich ELISA, competitive ELISA, ELISA strip or
Explain about the Importance of Vitamin E in Human Body? Vitamin E is the generic term for tocopherols and tocopherols that have a Phenolic functional group on a chromane ring
For a sample containing both protein and nucleic acid, the absorbance measured at a particular wavelenght will be the sum of contributions from both types of molecule. using the pr
Solubility and physical properties of protiens Simple proteins Conjugated proteins Derived proteins
How does the study of fossils strengthen the theory of evolution? The study of fossils reveals extinct and ancient species having many similar structures to others of the prese
To develop the food exchange list, a doctor or dietician keeps in mind four important basic points. These are: 1) Group similar foods in one group. For example cereals, roots,
Define Nutritional Management of Neurological Disorders Your friend's aged father, over a period of time has become very confused, forgetful and is unable to do many things he
E mb ryonic stem cells (ES): These are pleuripotent cells of early embryo (blastocyst) capable of giving rise to all differentiated cells, including germ line cells. Em
If NAD+ is an electron acceptor that becomes NADH and the NADH then donates a proton to ATPase, shouldn't NADH now become NAD- since it lost it's proton but still has the electron?
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