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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Foods Effect on Drug Transport? Many drugs are transported in blood bound lo plasma proteins. Severe malnutrition or diseases affecting the synthesis of plasma proteins
Syphillis Parenteral penicillin G remains the drug of choice for treating all stages of syphilis. Primary, secondary or latent syphilis known to be of less than one year's dur
A useful way to grow seeds Tie a piece of cloth over the mouth of an old potted meat jar. Permit extra cloth to hang down the sides and dip in about 2 cm of water contained in
Q. Management of Gout? The goals of therapy or management of gout is based on the following aspects: • early resolution of inflammation, • prevention of recurrent attac
Emerging and New Generation By-Products Oil palm by-products are good examples of NCFR which are becoming increasingly important new feeds. This is associated with the rapidly
What are the classes in Phylum coelenterata?
Food Gathering and Hunting : Food Gathering and Hunting In order to live, man needed to eat and to protect himself from the weather and animals. For both purposes he found it
who is the farther of bio diversity?
What is the typical localization of the tropical forests regarding latitude? The Tropical rain forests, like the Congo forest and the Amazon forest, are typically located in lo
GAS T RUL A TIO N - Morphogenic movements are occured. 3 germinal layers are formed. Ectoderm, endoderm & mesoderm. Endoderm is formed first. Mesoderm is formed in the
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