Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Medication for cardiac patients, Medication The patient is advised to ...

Medication The patient is advised to continue antibiotics for a week more after discharge. Other medications prescribed are analgesics (acetaminopen), cough syrup, diuretics

Gregor johann mendel, GREGOR JOHANN MENDEL 1.         Mendel was born o...

GREGOR JOHANN MENDEL 1.         Mendel was born on 22 July 1822 at Heizendorf in Austria at Selesia village. 2.         Mendel was worked in Augustion Monastry as monk at Br

Determine the genotypes of parents, In humans, the recessive allele that ca...

In humans, the recessive allele that causes a form of red-green colour blindness (c) is found on the X chromosome. a) Determine the genotypes and phenotypes of the F1 generation fr

Lymphatic system - circulation, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Define the axon of a neuron, Consider an axon of a neuron. At time=t1, its ...

Consider an axon of a neuron. At time=t1, its voltage is at threshold for an action potential; at time=t2, its voltage is at 0 millivolts prior to the peak of that action potential

What are blood stem cells, What are blood stem cells? Stem cells are un...

What are blood stem cells? Stem cells are undifferentiated cells able to distinguish into other types of specialized cells. The stem cells of the bone marrow create the diff

Protozoa, what is the classification of protozoa

what is the classification of protozoa

Describe the functional problems of implant failures, Functional Problems o...

Functional Problems of implant failures The masticatory efficiency of the implant supported prosthesis depends on the anchorage related factors and the prosthesis related fact

What are the main human diseases caused by fungi, Q. What are the main huma...

Q. What are the main human diseases caused by fungi? The major human diseases caused by fungi in immunocompetent patients are , blastomycosis, histoplasmosis, paracoccidioidomy

Heterotrophic nutrition, heterotrophic nutrition in any 5 type...

heterotrophic nutrition in any 5 types of organism

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd