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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Plankton - Aquatic Ecosystem This group includes both microscopic plants (phytoplankton) and animals (zooplankton) found in all aquatic ecosystems, except certain swift moving
Three charges are arranged as shown in the figure below. Find the magnitude and direction of the electrostatic force on the charge q = 4.78 nC at the origin. (Let r12 = 0.260 m.)
Rapid Crawling and Swimming Rapid Crawling This movement depends upon mainly on the contralateral waves of contraction of the longitudinal muscles of the body wall that
How are platelets formed? What is the function of platelets? What consequences does the clinical condition known as thrombocytopenia yield? Platelets, also called as thrombocyt
How potassium is essential for the following physiological functions It is vital for photosynthesis. When K + ion is deficient, photosynthesis declines and respiration incr
Ask question #M1. Discuss why Obelia is considered to be of special interest in Zoology as an animal showing an intermediate grade of organization. inimum 100 words accepted#
Q. Finding the Problem by communication process? - Open up the patient with general talk. - Initiate topic easy to talk. - Ask simple questions easily understood. - As
Nutrition Monitoring and Evaluation Monitoring and evaluation is an essential step in the nutritional care process and is very important too. Monitoring specifically refers to
Define Methods of Determination of Proteins and Amino Acid Content in Foods? The methods for protein quality evaluation are grouped under the following headings: I) Analytic
Q What is the function of the skin in humans? The skin is the external covering of the body. In humans its major functions are protection and perception of information from the
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