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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What are pioneer species? What is the role of the pioneer species? The Pioneer species are those first species that colonize places where previously there were no living bei
Q. Explain about Dietary Management? Treatment for gout often include a diet of lower purine intake. Indeed, about one third of the body's uric acid can be attributed to diet.
Zymogen :- an inactive form of an enzyme; becomes active prior to its action.
Define the meaning of Population Structure? The structure of population can be viewed as to how the population is composed of in terms of age and sex. The structure of populati
what is nitrogen cycle?
Solubility of Oxygen It is also important to know that solubility of gases decreases with the presence of solutes or increased salinity. Table shows the solubility of oxygen a
Define the Physical and Chemical Factors That Affect the Pasteurization? There are a few other physical and chemical factors that affect the pasteurization method. These involv
Classify phylum Aschelminthes
Question 1 List the cells and organs involved in immune system. Explain the role of thymus in development of T cells. Add a note on recognition of self antigens by immune system
Solid waste management, Other Management The solid wastes from a summer camp with 100 children and a staff of 25 are to be collected once per week. If bottles and cans (representi
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