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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
State about polyploid cells During development cells of certain tissues like the salivary gland and liver produce extraordinary amounts of selected proteins. This is often achi
Define Feeding and Nutritional Management for Neuro trauma? The main objective of nutritional management is to counteract the hypermetabolism associated with inflammation. The
Why is the cerebellum more developed in mammals that jump or fly? The cerebellum is the major brain structure that coordinates the movement and the equilibrium of the body. For
Define the Incubator - Food Microbiology? The growth of the microorganisms is altered by the chemical and physical nature of its surrounding. Most important environmental featu
Biological Responses Controlled by Phytochrome Phytochrome responses are those which are controlled reversibly by red and far-red light. These can be broadly categorised as:
The marine biomes consist of the earth's oceans and its associated areas like the shorelines, islands, reefs and estuaries. The marine waters contain about 3.5 percent salt, mostly
Detritus Food Chains Detritus food chains begin with dead organic matter which is an important source of energy. A large amount of organic matter is contributed by the death o
Observing where soil bacteria live Dig up a clover, alfalfa or soy-bean plant. Carefully increase all the soil from the roots and see if you can find the little white nodules o
mode of nutrition in rose plant
Adrenal causes of hypertension are: 1) Excess of aldosterone production in primary aldosteronism. The diagnosis may be suspected when persistent hypokalemia is detected. Most of
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