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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
NON-STARCH POLYSACCHARIDES A polysaccharide often termed as complex carbohydrate. Besides starch, a mixture of substances called non starch polysaccharide (NSP), also constitut
PRODUCT S OF HEMP PLANT - Four drugs - bhang, ganja, charas & marijuana obtained from Cannabis indica and C. sativa of family Moraceae . Main alkaloid is tetrah
what are the general characters of chordates? At least 10 characters.
The human abo blood type codominant alleles a and b are dominant to O. A man with blood type B and women with blood type A could potentially have children with which of the followi
Aplasia of epidydimis This condition is seen in only 1 or 2 % of young bulls and is marked by absence of one (more common) or both epididymis. Bulls having a single epidydimis
Excretory organs of agama lizard
Counter current flow - Respiration Each filament has an upper and a lower row of flat lamellae. The lamellae of successive filaments are in close contact. Gas exchange takes p
Define Foam Spray Drying Method? This is an extension of spray drying and involves the use of gases dissolved under pressure prior to spraying. The main advantage is that the
What is neiosis
Q. Proteins requirement in dyslipidemia? Proteins: While the quantity of protein does not impose any significant impact on the serum lipoproteins, it is the quality of protein,
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