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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
why are proteins so versatile?
Arboriculture : It deals with the cultivation of ornamental plants. Arboriculture is the cultivation, management or study of individual shrubs, trees, vines, or other perennial woo
Define the Food Sources of Thiamin? Thiamin is present in many food products and depending on the amount of vitamin present, we have categorized the foods as rich, good or fair
Epimorphic Regeneration In this sort of regeneration the lost part is reformed and restored via the growth of a bud or blastema from the remaining part of the organism followe
Isolation and identification of the etiological agent: Isolation of the etiological agent is attempted in chicken embryos, cell cultures, laboratory animals, culture medi
Antinutritional factors Unconventional feed resources including many agriculture by-products are rich in antinutrients and toxic constituents, and tannins being the most widely o
Explain the Evolution of the Vascular Plant Body? Vascular Plant anatomy reflects adaptation to life on land. In an aquatic environment, the photosynthetic surfaces are support
The DNA replication is a biological procedure which happens in all living organisms and copies their DNA; it is the basis for biological inheritance. The process will starts when o
Which are the main positive ions found in living beings? The major cations found in living beings are the sodium cation (Na+), the potassium cation (K+), the calcium cation (Ca
information on climbing beans
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