Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Pollen tube structure, Pollen Tube Structure The pollen tube in the s...

Pollen Tube Structure The pollen tube in the stigma is filled with cytoplasm containing numerous mitochondria and dictyosomes. The number of dictyosome cisternae is reduced i

Define protection against poisoning - function of vitamin e, Define Protect...

Define Protection against poisoning - Function of Vitamin E? 1) Protection against poisoning: It protects liver from injury due to carbon tetra chloride poisoning. 2) Pr

Explain digestion and of digestive system of platyhelminthes, Q What are th...

Q What are the kinds of digestion and of digestive system of platyhelminthes? Flatworms have incomplete digestive systems and they present intracellular and extracellular compl

Adverse effect to intestinal mucosa, Q. Adverse effect to intestinal mucosa...

Q. Adverse effect to intestinal mucosa? Alcohol: Alcohol is known to cause damage to intestinal mucosa independent of gastric acid content. Thus high amounts are not advised.

Describe a. the factors responsible for promoting lactation, Describe a. th...

Describe a. the factors responsible for promoting lactation, and b. the mechanism responsible for the cessation of lactation when a mother weans her baby.

Explain the flavour binding, Explain the Flavour Binding? Some protein ...

Explain the Flavour Binding? Some protein preparations, although acceptable from a functional and nutritional stand point, necessitate a deodorizing step to remove the bound of

How many kcal of gross energy were formed, The question asks . . . 18 moles...

The question asks . . . 18 moles of moles of maltose are completely oxidized by the reactions of the final oxidative pathways (Embden-Myerhoff Pathway and Krebs Cycle). How many AT

Group of the phylum chordata, Q. What is the group of the phylum Chordata t...

Q. What is the group of the phylum Chordata that first colonized the terrestrial environment? From which habitat did they come? Partially aquatic partially terrestrial animals,

Procedures for diagnosis - monoclonal antibodies, M o n o c ...

M o n o c l o n a l antibodies: Hybridoma technology for production of monoclonal antibodies is one of the most important advancements in the f

Define the methods of concentration, Define the Methods of Concentration? ...

Define the Methods of Concentration? We define the concentration process as one that starts with a liquid product and ends up with a more concentrated, although still liquid a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd