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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Metabolic response to food - Requirement of Energy? Eating requires energy for the ingestion and digestion of food, and for the absorption, transport, inter conversion,
Three point charges are located at the corners of an equilateral triangle. Find the magnitude and direction of the net electric force on the 1.60 uC charge. (Let A = 1.60 uC, B= 6.
Q. What are the periods of the second meiotic division? Meiosis II is divided into metaphase II, prophase II, anaphase II and telofase II.
Clostridium botulinum is an anaerobic, Gram-positive, spore forming, rod shaped bacteria that produces the potent neurotoxin. The organism and its spores are widely distributed in
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Explain about the Obliques muscles The superior and inferior oblique muscles form an angle of about 51 0 with the optical axis. The oblique muscles produce cyclorotation becau
If given this in this sequence to find surface area, 1 cm x 2 cm x 2 cm block, am I to assume it is written in LxWxH format? I actually think the length is 2 though and the width i
Describe the Physiology of atrial septal defect? In ASD the magnitude of left to right shunt is determined by the size of the defect and the relative compliance of the right ve
What are the constituent elements of the blood? The blood is made of a liquid and a cellular part. The fluid part is known as plasma and in it there are several substances, suc
3. Which substance(s) crossed the dialysis membrane? Support your response with data-based evidence.
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