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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Features of Plant Hormones The following are the important features of the role of plant hormones in growth and development: The hormone may initiate one response
What are the factors that limit our ability to fully classify and understand microbial diversity and what implications do these challenges have for environmental and health managem
Q. What are melanocytes? Melanocytes are epithelial cells of the skin specialized in secretion of melanin and Melanin is a pigment that besides coloring the iris of the eye and
Q. Define osseointegration and its theories? Osseointegration implies that "it is a contact established without interposition of non bony tissue between normal remodeled bone a
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comparitive chart on different types of respiration obserbed in different type of respiration organ of respiration , types of respiration,energy obtained , by product formed
Vitamin B 12 (Cyanocobalamin) Vitamin B 12 refers to a group of Cobalt-containing corrinoids known as cobalamins. It is also called antipernicious- anemia factor, extrinsic f
How were the classification systems of Aristotle and Linnaeus similar? Both systems divided all living things into two major groups, animals and plants, but Aristotle's system
What is Burden of Rheumatic Heart Diseases? Although in the twenty-first century RHD has been eradicated in western countries, in India and other developing countries it contin
what year was homo erectus fossils found
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