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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Which of the following statements concerning the inheritance of a dominant trait is true? Every affected person must have at least one affected parent. The trait is observed to ski
Your client has the flu and reports 5-6 loose stools a day. He has experienced an isotonic fluid volume loss. Explain what an isotonic fluid loss means.
Transgenic Animal Over the centuries, animals with new combinations of genes have been produced using conventional breeding methods by means of careful selection of particular
Give the causative organism and symptoms of Bacillary Dysentery • Bacillary dysentery is caused by Shigella sp. (Shigella sonnei, S. dysenteraei). Incubation period is
What is the type of reproduction that occurs in roundworms? What typical element do nematode sperm cells have? Nematodes reproduce sexually. The nematode sperm cell does not h
what is vagina explain with diagram
Antibody Structure: An antibody molecule consist of two alike light chains ( 220 amino acids each) ad two similar heavy chains (about 440-450 amino acids each) held together
Q. Define Diabetic Ketoacidosis? Diabetic Ketoacidosis (DKA) is one of the acute complications of diabetes mellitus. The name itself implies that there is acidosis (decrease in
You have a 1M KCl stock solution and a 85 mg/ml sulphuric acid stock solution. How will you make a 200 ml single solution containing 20mM KCl and 18 mg/ml sulphuric acid?
Define Sit and Reach Flexibility component in the humans? The subject sits on a mat with legs extended straight ahead. Legs should be at right angles to a taped the r box, with
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