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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
State the Diagnosis and prevention of VIBRIO PARAHAEMOLYTICUS Diagnosis: Diagnosis of gastroenteritis caused by this organism is made by culturing the organism from the diarrh
Explain what is pulse ? Examination of peripheral pulses as a very important step in cardiovascular examination. A normal pulse has upstroke (anacrotic limb) followed by a down
Epithelial tissue the cells in animals which are closely packed in either single or multiple layers, and which cover internal and external surfaces both of the animal body. It is
what is the life cycle of plasmodium vivax?
Explain about the Obliques muscles The superior and inferior oblique muscles form an angle of about 51 0 with the optical axis. The oblique muscles produce cyclorotation becau
A simple osmometer Remove the bottom from a small glass bottle about 2.5 cm in diameter. Fit a one-hole stopper tightly into the bottom and put a 50 cm length of glass tubing o
Patient after cardiac transplant The major focus of medical and nursing care after transplantation is to prevent early identification of rejection so that appropriate
Approaches to Neuropsychological Assessment Probably the most useful model of neuropsychological interpretation is described in Reitan and Wolfson's (1993) four approaches to a
Life cycle of Filarial worm Filariasis : It is popularly known as elephantiasis. It is caused by a nematode parasite, wuchereria bancrofti, and W.Malayi. Parasite : Wucher
What are cells with a delimited nucleus called ? What are the main elements of the nucleus? Cells with delimited nucleus are known as eukaryotic cells. Organisms composed of on
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