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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Which of the following is true for the ATP-sensitive potassium channel? A. The channel is a spanning protein with a receptor site for ATP located on an extracellular region of
The quartenary structure of a holoenzyme contains four long polypeptide subunits composed of non-polar, polar and charged amino acids. All subunits are properly folded and function
Define Principle of fehling test - reduction tests? Sugars that possess a free or potentially free (those that can be converted to free) aldehyde or ketonic group have a proper
recessive epistasis
H yd r o p e r i c a r d i um - h e pa titi s syndrome Also known as 'Angara disease' or 'leachy heart disease', this complex illness of poultry spread rapid
The different organs of the respiratory system Nostrils through which we inhale air in the nasal cavity Pharynx Larynx Trachea (wind pipe) Lungs - Bronchi,
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Describe TR Murmur and VSD murmur in details? TR Murmur : Characteristic: Holosystolic murmur, loudest at left lower-sternal border and becomes louder with inspiration (Carv
Enantiomers: Select one: a. Have the same molecular weight b. Have the same connectivity c. Are mirror images d. Are non-superimposable e. All of the above
Explain exotic species? Describe with the help of two examples how the exotic species disturb the native species of an ecosystem ?
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