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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define the Classification of Proteins? Proteins vary widely in. their properties. We suggest you look up this unit once again now as the information about proteins and their pr
Task: Write a maximum of five (5) pages of your research plan which should include details on the following topics as appropriate to your chosen study design: I. Background info
Explain the requirement of Nutrition during Stress? We all experience stress at some time or the other in life. Stress is the condition or stimulus that threatens the body's ho
GER M LAYER THEORY - Proposed by Pander, studied chick embryo development. Coined the term ectoderm, mesoderm & endoderm.
what is meant by double penetration sex?
Thermodynamics Thermodynamics tells us that every transformation of matter chemical transformation is accompanied with energy conversion .For example when complex molecul
I nfe c tio u s laryngotracheitis It is a slow, laterally spreading respiratory disease of chickens, pheasants, peafowl and turkeys caused by a herpes virus with high morb
Explain the Invasive species? Tens of thousands of non-native species have been introduced into the United States of America, with more arriving each year. These species can ha
Explain the Primary Stain - Ziehl-Neelsen Method? Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. C
Define Precautions for estimation of vitamin c in a solution? 1. Rinse all glassware with 3% metaphosphoric acid before you begin your practical and subsequently each time you
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