Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Zoonotic diseases-yellow fever, Yellow fever Yellow fever also known a...

Yellow fever Yellow fever also known as Black Vomit and American plaque is a viral disease primarily of monkeys and transmitted to man by female Aedes mosquitoes. It is caused

Explain free water - water found in food, Explain Free Water - Water Found ...

Explain Free Water - Water Found in Food? Water which is bound by such minute forces, that its vapour pressure is equal to the vapour pressure of pure water. It can be found as

Common respiratory disorders, COMMON RESPIRATORY DISORDERS: Respiratio...

COMMON RESPIRATORY DISORDERS: Respiration is one of the most vital functions of the body. The purpose of respiration is to provide oxygen ta  the body  cells and to remove exc

Explain light in cell metabolism, Explain Light in cell Metabolism? Ene...

Explain Light in cell Metabolism? Energy for almost all organisms on earth is derived directly or indirectly from light energy produced by the sun. Sunlight consists of energy

Do membranes form only the outer wrapping of cells, Normal 0 fa...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Explain the acoelomates - animals without a body cavity, Explain the Acoelo...

Explain the Acoelomates - Animals without a Body Cavity? The simplest group of animals that has bilateral symmetry and a solid body (acoelomate) is the Platyhelminthes. Phy

Define effect of vitamins (a, Define the effect of Vitamins (A, D, K and B-...

Define the effect of Vitamins (A, D, K and B-Complex) on athletes? Vitamins A, D and K have been found to have no ergogenic effects. Ingesting large doses of these vitamins hav

What is supraventricular arrhythmias, Q. What is Supraventricular Arrhythmi...

Q. What is Supraventricular Arrhythmias? Such arrhythmia tend to be reduced by the vagal withdrawal accompanying the onset of exercise.This and wandering pacemakers tend to rec

What are the keys for identifying plants and animals, Q. What are the Keys ...

Q. What are the Keys for Identifying plants and animals? Keys are determined as devices to help in identifying plants and animals. These are merely an aid to identification bas

Why proteins interact with each other inside of a cell, What technique woul...

What technique would you use to see if two proteins interact with each other inside of a cell?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd