Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Calculate the width of the implant, Width of the Implant The width of t...

Width of the Implant The width of the implant especially at the interface area is critical towards the success of the implant. It has been recommended that at least 1mm of bone

Sphingolipids, What are Sphingolipids? Also there link to cancer caused in ...

What are Sphingolipids? Also there link to cancer caused in cells.

Explain difference between an ecological niche and a habitat, What is the d...

What is the difference between an ecological niche and a habitat? An ecological place is a set of peculiar activities, resources and strategies that a species explores to repro

What is the reason for the low red blood cell count, A patient has a low re...

A patient has a low red blood cell count, and microscopic examination reveals an abnormally high proportion of circulating reticulocytes. Upon subsequent examination, the patient i

Explain the false indications of endodontic surgery, Explain the False indi...

Explain the False indications of Endodontic Surgery a) Large periapical lesion that could not be eliminated with non-surgical approach. b) Incomplete root formation "open ap

Quantitative or analytical method for estimation of nitrogen, Define Quanti...

Define Quantitative or Analytical Method for Estimation of Nitrogen and Protein? You may recall reading earlier that proteins contain nitrogen along with carbon, hydrogen and s

Explain about natural pigments, Explain about natural pigments There ha...

Explain about natural pigments There has been an extensive search of the microbial, plant and animal kingdoms for pigments that possess both high tinctorial power/strength (a m

What would be the knowledge of regeneration, A 19 year old model for Glamou...

A 19 year old model for Glamour magazine tripped over an extension cord causing injury to her epidermis. She is afraid the injury will leave a scar. Based on your knowledge of rege

Respiration, how energy enters and then flows through a biologial system?

how energy enters and then flows through a biologial system?

Etiological factor of dyspepsia, Q. Etiological factor of dyspepsia? Th...

Q. Etiological factor of dyspepsia? The main etiological factor of dyspepsia is the failure of proper digestion and absorption of food in the alimentary tract and the consequen

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd