Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Define symptoms of overall wilting, If you observe symptoms of overall wilt...

If you observe symptoms of overall wilting, stunting, and thinning of the leaves, would you expect a foliar or root disease? Why?

Discovery of plant hormones, Discovery of Plant Hormones To date, five...

Discovery of Plant Hormones To date, five major classes of plant hormones have been discovered namely auxins, gibberellins, cytokinins, abscisic acid and ethylene. It is possi

Ecology.., write the charecteristics of lake ecosystem

write the charecteristics of lake ecosystem

Risk during crown removal-endodontics principles, Risk during crown removal...

Risk during crown removal -Tooth inadvertently extracted using a crown\bridge remover . -Endodontic therapy was performed , and the tooth was replanted , a procedure known

Corpus cardiacum - endocrine organs, Corpus Cardiacum - Endocrine Organs ...

Corpus Cardiacum - Endocrine Organs We before mentioned that corpus cardiacum (pl. corpora cardiaca) is a neurohemal organ in insects. It is gland of neural origin. They are c

Diffusion of gases, Diffusion of Gases Gases diffuse from areas of hi...

Diffusion of Gases Gases diffuse from areas of higher partial pressure to areas of lower partial pressure. The diffusion along the partial pressure gradient ceases only when

Water - properties and functions, W A TER Occurence Water is ...

W A TER Occurence Water is the most abundant substance, covering 70% of earth's surface. It constitutes 60-90% of living cells.              5-6% in tooth e

What is the role of the ozone layer for living beings, Q. What is the role ...

Q. What is the role of the ozone layer for living beings? The Ozone, O3, is a gas of the atmosphere that filters ultraviolet radiation from the sun disallowing most of that rad

Functions of stomata, Functions of Stomata The cuticle forms outermost...

Functions of Stomata The cuticle forms outermost surface of the leaf and offers resistance to the evaporation of water vapour and entry of carbon dioxide necessary for photosy

What is the conjugate acid, Calculate the pH: 300mL of 0.25M sodium ascorba...

Calculate the pH: 300mL of 0.25M sodium ascorbate plus 150mL of 0.2M HCl (the pKa of ascorbic acid is 4.04) okay, what's the conjugate acid and base of this problem? Can someone wa

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd