Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Explain third mitotic period, Q. What are the major events of the third mit...

Q. What are the major events of the third mitotic period? The third mitotic period is anaphase. In anaphase the following events occur breaking and duplication of centromeres w

Ciliates - parasitic protozoan, Ciliates - Parasitic Protozoan The onl...

Ciliates - Parasitic Protozoan The only ciliate of medical and veterinary importance is Balantidium coli which inhabit the large intestine of man, apes, monkeys and pigs. B. c

Which substance starts clotting in humans after a wound, When a wound occur...

When a wound occurs in humans, platelets in the blood activate a substance that starts clotting process. The substance which starts clotting is: a) Adenosine (pron: ah-den-ah-s

Endocrine system, INTRODUCTIO N - Endocrinolog y - Study of endocri...

INTRODUCTIO N - Endocrinolog y - Study of endocrine glands, hormones and their effects. Thomas Addison - Father of endocrinology. Described hormonal disease in 1855 kn

Protozoa, Characteristics Protozoa and examples

Characteristics Protozoa and examples

Explain the dimensions of practice of nutrition care process, The nutrition...

The nutrition care process acknowledges the common dimensions of practice by  the following: Defining a common language that allows nutrition practice to be more measurable,

Riccia, What isthe Riccia

What isthe Riccia

Class pycnogonida of phylum arthropoda, Class Pycnogonida of Phylum Arthrop...

Class Pycnogonida of Phylum Arthropoda Measures generally 3-4 mm; body chiefly made up of cephalothorax, abdomen being very small; generally four pairs of walking legs; long p

What is the normal duration of the menstrual cycle, What is the normal dura...

What is the normal duration of the menstrual cycle? How does the calendar contraceptive method work? The normal duration of the menstrual cycle is 28 days but it can vary betwe

Causes of variation, CAUSE S OF VARIATION - Effect of environmental co...

CAUSE S OF VARIATION - Effect of environmental conditions Change in the gene pattern like - (a) Random distribution of homologous genes in meiosis (b) Crossing over

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd