Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Vascular tissues, VASCULA R TISSUES (= FLUID TISSUES) - The vascula...

VASCULA R TISSUES (= FLUID TISSUES) - The vascular tissues are of two types: blood and lymph.

Transplantation, Transplantation - It is implanting of a living or p...

Transplantation - It is implanting of a living or preserved tissue or organ from one site to another side in the same individual or from one individual called donor to recip

What is the function of the skin in humans, Q What is the function of the s...

Q What is the function of the skin in humans? The skin is the external covering of the body. In humans its major functions are protection and perception of information from the

Explain the life cycle of water, Explain the life cycle of Water? The ...

Explain the life cycle of Water? The water cycle , or hydrologic cycle, is one of the most important processes to living organisms on Earth. Consider the following facts:

Pollution, Phosphate detergent as cause of water pollution

Phosphate detergent as cause of water pollution

Determine the extracellular concentration of tva, Determine the extracellul...

Determine the extracellular concentration of TVA Consider Neuron B in the frog central nervous system whose plasma membrane has a previously unknown channel that is selectivel

Virus, what is virus ?? what is classidication , uses , disease of viruses...

what is virus ?? what is classidication , uses , disease of viruses ? what are types of virus ?

Oxygen dissociation curves, Oxygen Dissociation Curves The oxygen cont...

Oxygen Dissociation Curves The oxygen content of blood fully saturated or oxygenated can be calculated. It is known as the oxygen capacity of blood and this varies for differe

Zoonoses disease-transmissible spongif orm encephalopathies, Transmissible ...

Transmissible spongif orm encephalopathies Transmissible spongiform encephalopathies (TSEs, also called Prion diseases) are fatal neurodegenerative diseases such as scrapie of

Determine the end of the meiotic process during oogenesis, What is the rela...

What is the relation among fecundation and the end of the meiotic process during oogenesis? The oocyte II only completes the second meiotic division (interrupted at metaphase) i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd