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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
CHARACTERSTICS OF CLEAVAGE - In cleavage involve the series of mitotic division, so daughter cells are genetically similar to the parental cell. The resulting cells are c
What is the cost-benefit relationship regarding sewage treatment as a method to fight water pollution? To treat sewage is much cheaper for society. The non treated sewage pollu
Illustrate Nernst equilibrium potential for potassium ions A complete motor neuron is removed from a frog and placed in normal physiological saline at 1 AM. The neuron is hea
Difference between other eukaryotic cells ? Cell Wall : In addition to the presence of photosynthetic plastids, another significant difference between other eukaryotic cell
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Q What are the two big groups into which cells are classified? Cells can be classified as prokaryotic or eukaryotic. Prokaryotic cell is that with no delimited nucleus. Eukaryo
Explain Pasteurization (temperature below 100° C) - method of food preservation? Pasteurization is a heat treatment that kills a part but not all the microorganisms present and
What is the difference between osmosis and diffusion? Osmosis is the occurrence of movement of solvent particles (in general, water) from a region of lower solute concentration
Preventive steps for global warming : Growing trees in vacant places. Growing social forests and protect them. Promoting abundant growth of phytoplankton plant species in marine
Explain the Ethological Approach in Taxonomy The use of behavioural or ethoIogica1 characteristics in animals is relatively new, though extremely useful, particularly in the id
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