Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Explain the functions of folate, Explain the Functions of Folate? Folate,...

Explain the Functions of Folate? Folate, also known as folic acid, is essential for good health. Folate requiring reactions include those involved in phases of amino acid metabol

SOIL, WHICH SOIL WILL BE FRUIFUL FOR GROWING LENTILS AND OTHER PULSES

WHICH SOIL WILL BE FRUIFUL FOR GROWING LENTILS AND OTHER PULSES

Illustrate the major cells of the nervous system, Q. Illustrate the major c...

Q. Illustrate the major cells of the nervous system? The major cells of the nervous system are the neurons. as well the neurons the nervous system is also constituted of glial

#classification of parasitic protozoa.., most recent classification of para...

most recent classification of parasitic protozoa with its general characters

Define principle for estimation of cholesterol - lipids, Define Principle f...

Define Principle for Estimation of cholesterol - Lipids? Zlatkis and associates have evolved a method of quantitative determination of cholesterol based on a reagent containing

What is an endospore - staining strategies, What is an endospore? An en...

What is an endospore? An endospore is a specialized, highly resistant, dormant structure formed within the vegetative cell of some bacteria e.g. Bacillus (rod), Clostridium (ro

Define the term parasite -giardia, Normal 0 false false fal...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

How do contraceptive pills generally work, How do contraceptive pills gener...

How do contraceptive pills generally work? Contraceptive pills generally have the hormones estrogen and progesterone. If taken daily from the 4th day after menses the abnormal

Define clostridium perfringens-associated gastroenteritis, Q. Define Clostr...

Q. Define Clostridium perfringens-associated gastroenteritis? Clostridium perfringens-associated gastroenteritis is a food borne disease which is frequently reported and is

State about led fredrick griffith, In the experiments that led Fredrick Gr...

In the experiments that led Fredrick Griffith to discover the "Transforming Principle", which of the following was the key step in illustrating that a substance within one cell cou

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd