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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Now try to pull the strands apart at the "origin of replication." What happens to the DNA molecule when the ends are restricted
Q. Managing diarrhoea in children? Managing diarrhoea in children is of great concern. The guidelines for the same are as follows: 1. The first objective is to rehydrate the
(a) What is the distinction among 'breathing' and 'respiration'? (b) What part do 'ventilation' and 'gaseous exchange' play in these processes? (a) Breathing i
What are the lateral meristems? Where can they be found and which kind of plant growth do they promote? The Lateral or the secondary, meristems, are the cambium and the phellog
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Consequences of Air Pollution The physiological effects of toxic pollutants on living beings can be of two types: Acute effects and Chronic effects. Acute effec
Define the Nutritional Assessment Tools? Malnutrition/protein energy malnutrition amongst elderly persons has been observed in various studies -be it hospitalized patients, nur
Explain the Working Principle of Spectrophotometer? The working principle of spectrophotometer is graphically presented in Figure. Figure: Working princip
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