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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Absorption of Dietary Iron? Haem iron is more bioavailable than non-haem iron because it is absorbed intact as a soluble complex by endocytosis (process whereby cells ab
Outline the steps involved in using bacteria to produce human insulin. The gene for insulin is 'cut' from the appropriate strand of DNA using restriction enzymes. Plasmids are
The AV node of a mammalian heart is destroyed. A. A depolarization in a cell in the left atrium will cause a depolarization of a cell in the left ventricle. B. The contracti
.respiration in animals
how many types of respiration are?define them also
ERRORS IN FERTILIZATION - 1. Polyspermy - When more than one sperms are fused in an ovum, this condition is called polyspermy. 2. Polygynae - During oogen
Honors A&P assignments
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How the Oxidation-reduction potential influence the spoilage of meat? You learnt earlier that the presence or absence of oxygen and the 0-R potential of the food itself has a b
Respiratory Insufficiency Respiratory insufficiency usually indicate inadequate exchange of oxygen and carbondioxide to meet the needs of the body during normal activities.
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