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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Assume that you have 1 ml of a solution of amylase (an enzyme) at a concentration of 15 mg protein/ml. Calculate the volume of diluting buffer that you would have to add to 1.0 ml
What are quantitative data? Give two examples of quantitative data. Quantitative data are data that can be calculated in numbers. Examples contain the dimensions of an objec
Epithelial tissue the cells in animals which are closely packed in either single or multiple layers, and which cover internal and external surfaces both of the animal body. It is
Q. What is Fenugreek seeds? Fenugreek seeds: 'Commonly known as methi seeds in Hindi. They are commonly used in Indian cuisine in chutney and even in pickles and several dishes
Q. Describe Induced Ventricular Arrhythmias? The prognostic significance of ventricular ectopy provoked by stress testing after an MI is controversial. Complex ventricular arrh
Why does bark often die and break naturally? The bark is the mature periderm of the stem, branches and roots. It dies and breaks when these structures grow and thus the perider
WHY CONSERVE WILD LIFE ? 1 . ECONOMIC IMPORTANCE OF WILD LIFE - (i) Plants - (ii) Animals - (iii) Micro organisms - 2 . ECOLOGICAL BALANCE -
Person X is a healthy human who has volunteered to take experimental drug Y. Person X has a normal dinner at 6 PM on April 1 and then does not eat for 12 hours. At 5 PM on A
What is the difference between anabolism and catabolism? Metabolism comprises two opposing processes: anabolism and catabolism. Anabolism is a set of synthesis reactions that t
Basic Physical Concepts of Plant Water Relation The main idea of this section is to explain the concept of chemical potential of water or water potential and the effect of var
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