Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Cell, the s phase of interface is important and a cell can newer divide wit...

the s phase of interface is important and a cell can newer divide without it justify?

Explain back pain, Explain Back pain, arthritis and gout - Effect of Obesit...

Explain Back pain, arthritis and gout - Effect of Obesity? Abdominal obesity increases the risk of back pain because of the extra load on the spinal column. This, in turn, red

Mcnaughton case and law, MCNAUGHTON'S CASE AND LAW: In 1843, McNaughto...

MCNAUGHTON'S CASE AND LAW: In 1843, McNaughton shot and  killed Sir Robert Peel's secretary Edward -Drymmond.  It was  shown  that McNaughton suffered from a number of  delusi

Functions of the musculoskeletal system, Q. What are the functions of the m...

Q. What are the functions of the musculoskeletal system? The musculoskeletal system has the functions of protecting and supporting organs, maintenance of the body spatial confo

Effect of insulin on metabolism of carbohydrate, The foods we take have thr...

The foods we take have three basic ingredients - carbohydrate, protein and fat. Insulin has profound effects on the metabolisms of carbohydrate, protein and fat. Metabolism helps o

Diffusion, applications of diffusion

applications of diffusion

Which respiratory adaptations or organs do, Q. Which respiratory adaptation...

Q. Which respiratory adaptations or organs do aquatic and terrestrial arthropods respectively present? In crustaceans, typical aquatic beings, there are richly vascularized gil

Explain vitamin B complex, Vitamin B Complex The vitamin B complex comp...

Vitamin B Complex The vitamin B complex comprises the vitamins B 1 , B 2 , B 6 and B 12 as well as the vitamin B factors biotin, folic acid, nicotinic acid and its amide as w

Speciation, SPECIATION - Origin of new species: An isolated popula...

SPECIATION - Origin of new species: An isolated population of a species independently develops different types of mutations. The later accumulate in its gene pool. Afte

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd