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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
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Wind Strong current of air is known as wind, it is an important ecological factor as it affects plant life mainly on flat plains, along sea coasts and at high altitudes in moun
Question 1 A patient is admitted to the hospital with suspected pneumococcal pneumonia. How would you confirm this initial diagnosis? Answer the following questions a) Which is
Digestion of carbohydrates Carbohydrate digestion in vertebrates and invertebrates is very similar. All the enzymes shown in Table are not required by all animals. The enzymes
Q. Categories of value of biodiversity? The value of biodiversity is often divided into two main categories: · UTILITARIAN (also known as instrumental, extrinsic, or use) v
What is the difference between an acid and a base? Base is anything, which has a capability to abstract a proton. Using the easiest definition, an acid is something when add
Annexin V is a protein that binds to Phosphatidylserine. Why is this useful in the lab? Does it have to do with testing whether the cell is in normal condition?
Define Treatment and Management of Adverse Food Reactions? Management of adverse food reactions involves diagnosing the problem followed by nutritional and medical care.
Role of Endarterectomy : Endarterectomy is done as a semi open technique. 5-10mm long incision is made on the outer layers of the coronary artery and plain developed in 11ic thic
Define the Food Pricing? The pricing of the food products bought by the consumer is subject to multifarious factors. Each of the factors as discussed below can affect the prici
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