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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Considering a pair of homologous chromosomes containing a gene having two different alleles how many different genotypes can the individual present? If a gene of a diploid spec
Eutrophication - Effect on Water Bodies Moderate quantities of sewage decay naturally in a water body and water gets purified after some time. But the problem arises when the
Explain Total Parenteral Nutrition OPN) It is a method of providing, complete nutritional support in which the gastrointestinal (GI) tract is bypassed by introducing assimilab
Q. To which heart chamber does the blood go after leaving the left atrium? What is the valve that separates these compartments? The valve between the left atrium and the left v
Explain the types of Carrageenan It was first produced commercially from the red algae, Chandrus crispus, found along the northeast shores of the U.S and Canada and referred t
How Pathologic Conditions causing the underweight? Illness can affect weight status in a number of ways. For example, fevers and infections increase the demand for energy, whic
Write on spoilage of food products by fungi,nutrition,condition for growth,consequencies and prevention.
Q. What is the difference between the exocrine gland and the endocrine gland? Endocrine gland is a gland whose secretions are called as hormones that are collected by the blood
D-glucose differs from D-galactose only in the arrangement around carbon 4. Therefore D-glucose and D-galactose are- Select one: a. enantiomers b. epimers c. mirror ima
Poriferans and cnidarians do not have excretory systems. Do platyhelminthes have an excretory system? Platyhelminthes have a primitive excretory system made of flame cells (als
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