Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Mode of nutrition , What is the mode of nutrition in fish,human,amoeba,scor...

What is the mode of nutrition in fish,human,amoeba,scorpian & toad ?

How to monitor body weight to enhance athletic performance, How to monitor ...

How to monitor body weight to enhance athletic performance? Monitoring body weight is a practical way to assess energy balance. Weight stability, particularly during periods

List the heterotrophic nutrition in plants, a) List the a variety of modes ...

a) List the a variety of modes of heterotrophic nutrition in plants. b) Define any two modes giving one example of each.

Determine the appearance of a plant cell, Determine the appearance of a pla...

Determine the appearance of a plant cell when water is plentiful? When water is plentiful, the central vacuole expands. The other organelles are pushed against the plasma me

What do you mean by bleeding index, Q. What do you mean by Bleeding Index? ...

Q. What do you mean by Bleeding Index? This factor is applicable at this stage to evaluate the health of one stage implants in which the transmucosal component allows the forma

Sickle Cell, Sickling occurs in deoxyhemoglobin S, but not in oxyhemoglobin...

Sickling occurs in deoxyhemoglobin S, but not in oxyhemoglobin S. Oxyhemoglobin has a small hydrophobic \"pocket\" in a ß chain region located in the interior of the protein. In de

Which are plant tissues that cover the stem and the leaves, Which are plant...

Which are plant tissues that cover the stem and the leaves? The stem perhaps covered by epidermis (having stomata, cuticle and photosynthetic cells) as in monocots or, alternat

What is active transport , The active transport of molecules requires a...

The active transport of molecules requires an input of metabolic energy and this can  be  derived  either  from  direct  coupling  to  the  hydrolysis  of  ATP  or  by coupling to

Explain the endochondral ossification, Explain the endochondral ossificatio...

Explain the endochondral ossification The second type of osteogenesis is endochondral ossification. This process is different from intramembranous ossification in that it occur

Insects - hormones in growth and reproduction, Insects - Hormones in Growth...

Insects - Hormones in Growth and Reproduction In insects hormones regulate moulting and metamorphosis. The larvae or nymphs which hatch out of the eggs undergo regular moultin

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd