Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Genetics, GENETICS 1.         The branch genetics started in 1900 after...

GENETICS 1.         The branch genetics started in 1900 after rediscovery of Mendel's work. 2.         Gregor Johann Mendel is called Father of genetics. 3.         The t

Microbiology, general characteristics of cynobacteria

general characteristics of cynobacteria

What is enteral feeding, Tube or Enteral Feeding Ideally the patient mu...

Tube or Enteral Feeding Ideally the patient must be fed orally, but in cases where the patient is unable to take solid foods, a part or all of intake is usually given by the tu

Determine organic material for the energetic metabolism, How are bacteria c...

How are bacteria classified according to the production of organic material for the energetic metabolism? Most bacteria are heterotroph, they do not produce their own food. The

Describe three ways that rna differs from dna, Describe three ways that RNA...

Describe three ways that RNA differs from DNA. RNA is single stranded; DNA is double stranded all of the codons from the deletion point to the end of the transcript would be s

How physical activity affect requirement of protein, How Physical Activity ...

How Physical Activity affect requirement of protein? Research on protein needs for muscular work had its beginning in 1863 when Von Leiberg postulated that muscle protein was d

What are the factors to an actual shift in the supply curve, What are the f...

What are the factors to an actual shift in the supply curve? Factors which cause an actual shift in the supply curve are described in below: When prices rise or fall this wo

Define about the metabolism of copper, Define about the Metabolism of Coppe...

Define about the Metabolism of Copper? In food, most copper is present as Cu 2+ and some as Cu 1+ . This copper is bound to organic compounds especially protein. Gastric HCI p

Ruminate endosperm - variants of endosperm, Ruminate Endosperm - Variants o...

Ruminate Endosperm - Variants of Endosperm In certain plants the surface of the mature cellular endosperm shows a high degree of irregularity and unevenness, giving a ruminate

Define the protective of vitamin c role as an antioxidant, Define the Prote...

Define the Protective of Vitamin C role as an antioxidant? Vitamin C is a powerful antioxidant because it can donate a hydrogen atom and form a relatively stable ascorbyl free

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd