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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Amphibian frog embryo
Define the Structure of D and L isomerism? The prefix D or L are used to refer to the configuration of the carbon next to the primary alcoholic group or in other words, the con
Taeniasis Taeniasis is a zoonotic disease caused by specific cestodes. The two parasites of major importance are Taenia saginata and T. solium. Both in their adult forms, are
Or nit h o s i s (psittacosis) This is an important zoonotic bacterial infection and causes disease in humans and birds. Collectively, these conditions are called as chla
Locomotion and reproduction in echinodermata
Q. Does Ascaris lumbricoides present an intermediate host? An Ascaris is a monoxenous parasite, its life cycle is dependent only on one host and so it doesn't have intermediate
Using an average wavelength of 450 nm (0.45 um) can you resolve two objects that sit greater than 200nm apart from one another? Use mathematical calculations to justify your answer
Q In which environments bacteria live? Bacteria can be found in a variety of environments throughout the planet. There are bacteria in the air, on the surface, in fresh water,
Which of the following is true for active hyperemia, a local control mechanism within the circulatory system? A. There will be a decrease in force developed by smooth muscles s
Q. Why is rubella during gestation a threat to the fetus? If taking place during gestation rubella is a dangerous disease because the virus crosses the contaminates fetus and t
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