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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Cake mix evaluation Originally, cake mix formulations were very similar to bakery cakes and utilized standard "Hi-Ratio" cake oils; however, development of improved cake mixes
What was the Oral Status of the Patient It is imperative that the patient carry out a strict oral hygiene regimen as dental plaque is one of the main factors that leads to impl
Flowering - Development of plant One of the major changes that occur during the life cycle of a plant is the transition from vegetative stage to the flowering stage. In this t
Because of developmental abnormality, the wall of left ventricle of an infant's heart is as thin as that of right ventricle. What would be its explicit effect on circulation of blo
Why does one need to study about soil and what is its importance? You would be aware that, from the dawn of agriculture, cultivators were attracted to fertile soils of river
Q. What are the functions of the musculoskeletal system? The musculoskeletal system has the functions of protecting and supporting organs, maintenance of the body spatial confo
The symptoms and signs of endocarditis are often constitutional and, when localized, often result from a complication of IE rather than reflect the intracardiac infection itself. C
Individual components of biodiversity provide an unbeliev able wide range of products that are used to enhance the lives of people in all countries of the world. It is the diversit
• Cancer cells do not respond normally to the body's control mechanism. o They divide excessively and invade other tissues. o If left unchecked, they can kill the organism. •
Q. Primary prevention of diabetes mellitus? Approaches to Prevention: There are various approaches to prevention. Four levels of prevention related to different stages of a di
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