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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
List the benefits of exercises. Benefits of exercise are : - improves sugar control - decreases HbA1c level - decreases blood pressure - decreases triglyceride lev
Define about the Absorption of Iron? Before it can be absorbed, iron whether it is in the form of haem or non-haem must be released from the food matrices where it is bond with
Nitrogen Fixation As we have said before, atmospheric nitrogen cannot be used by plants or animals. It has to be first fixed. The term nitrogen fixation refers to the oxidatio
Define Proline It is a prominent amino acid found in fish and may contribute to sweetness. The sugars ribose, glucose and glucose-6-phosphate are flavour contributors, as is 5-
Question 1. State the basic properties of light. Discuss the special features of ultra-microscopy and phase contrast microscopy 2. What are ultrasonic waves? Explain the dif
Second Stage of Invagination The 2 nd stage of invagination starts with release of secondary mesenchyme from the tip of archenteron into the blastocoel. The cells of secondar
Medical Management Trealment of choice is cephalosporin which is given interavenously, alternatively can be given. In case of increased intra cranial pressure mannitol and o
What is Mega sporogenesis
Three point charges are located at the corners of an equilateral triangle. Find the magnitude and direction of the net electric force on the 1.60 uC charge. (Let A = 1.60 uC, B= 6.
What is Double Labelled Water (DLW)? Technique DLW is currently considered the most accurate technique for measuring TEE in free- living individuals. It is the method used to m
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