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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
does earthworm have rbc.
Define Keratomalacia - Micronutrient Deficiencies? This is a condition of rapid destruction and liquefaction of full thickness of cornea, leading lo prolapse of iris, resulting
Elaborate the following term in detail - Macronucleus. One of two types of dimorphic nuclei found in ciliate protozoans. Macronucleus contains multiple copies of genome (polypl
what is the taxonomic classification for the protist Archaeplastida?
Class angiospermae (Flowering plants) Flowers are the reproductive structures. Ovules are protected within ovary, xylem vessels are present. After fertilisation the ovary develop
Scrapie This is a chronic fatal disease of sheep and occasionally of goats caused by a Prion agent similar to BSE. The disease is reported from Europe, North America and in a f
List the four phases of meiosis I, and briefly explain what occurs during each phase Prophase I: DNA coils into chromosomes, the nucleolus and nuclear envelope disappear, the m
Q. What is the crossing over? And In which period of meiosis does this event occur? Crossing over is the eventual exchange of chromosomal fragments among homologous chromosomes
Q. Under which forms is the Trypanosoma cruzi found in its hosts? In the definitive hosts also as in triatomine bugs (intermediate hosts) the protozoan that causes Chagas' dise
Sexual reproduction in Eukaryotes In most eukaryotes, especially higher animals, individuals normally exhibit one or two sex phenotypes; female or male. In such species, females
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