Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Determine the testing for albumin in urine, Determine the Testing for Album...

Determine the Testing for Albumin in Urine Acetic Acid Test About 2 - 8 mgm/dl of protein is excreted in urine which is not usually detected by routine test. The testing o

What is the numeric relation between purine bases, Q. What is the numeric r...

Q. What is the numeric relation between purine and pyrimidine bases in the DNA molecule? Is that relation valid in the RNA molecules? The DNA molecule is made of two bound poly

Find the genotypes and phenotypes of the f1, Determine the genotypes and ph...

Determine the genotypes and phenotypes of the F1 generation from a colour blind father and a mother who is homozygous for normal colour vision.

Illustrate the types of Bone Remodeling, Illustrate the types of Bone Remod...

Illustrate the types of Bone Remodeling Bone remodeling occurs on existing bone surfaces. However, unlike modeling, remodeling cannot cause large changes in bone structure at a

Isolating mechanism, In this section we are to discuss the means by which p...

In this section we are to discuss the means by which populations get separated or isolated from each other, first gain the status of'Sub-species and finally evolve mechanisms which

Auxins - plant growth substances, Auxins - Plant Growth Substances Jul...

Auxins - Plant Growth Substances Julius Sachs (1882) first suggested on the basis of his observations that there are special substances for forming roots and some special subs

Define the obesity in children, Define the Obesity in Children? It is d...

Define the Obesity in Children? It is difficult to measure overweight or obesity in children and adolescents because they grow and mature at different rates. Weight status in c

Types of cell respiration, Q What are the kinds of cell respiration? Th...

Q What are the kinds of cell respiration? There are two types of cell respiration aerobic cell respiration a reaction with participation of molecular oxygen (O2) and anaerobic

Explain deet, DEET DEET is available in the US in many formulations wi...

DEET DEET is available in the US in many formulations with concentrations of 5%-40% and 100%; higher concentrations present complete protection for a longer period of time, bu

Modern biochemistry and cell physiology, This problem set is a little diffe...

This problem set is a little different, in that it is one large project/problem. To answer it, you must write a short single-spaced typed report of 4-5 pages MAXIMUM. Here is the p

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd