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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
describe five features of chromosomes used in cytogenetic analysis?
what is role of macro and micro nutrients
Advances in Mineral and Vitamin Nutrition of Livestock Even though, minerals and vitamins as nutrients are as essential as energy and protein, general negligence occurs primari
Q. Tools used in Taxonomy? Before you plan for a field trip decide the tools and equipment required. Certain, equipments are necessary for plant collection. A collecting pick i
Sources of vitamin C It is found in many of the natural foods like fresh citrus fruits like limes, lemons and oranges are rich sources of vitamin C. The best and the chea
Measurements of the level of pollution There are many factor for finding the level of pollution in the waste waters that generally find out the quantity of organic matter susp
Fl u o r osi s Continual ingestion of small amount of fluoride through feed or water leads to fluorosis in animals. The toxic effects are based on amount of fluorine inges
What is cyclosis? Cyclosis is a type of internal cell movement in which an oriented flow of circulating material is formed and maintained in the cytoplasm by the action of micr
Q. Investigations process of constrictive pericarditis? Electrocardiogram Low voltage complexes can occur. Left atrial enlargement may be seen. Atrial fibrillation occu
Explain what is Enzymes? Enzymes are organic substances that speed up, or catalyze, a chemical reaction. At a given temperature, molecules have varying amounts of energy, and
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