Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Bovine spongiform encephalopathy (bse), Normal 0 false fal...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Classification of plant on the basis of their resemblances, Q. Classificati...

Q. Classification of plant on the basis of their resemblances? Pliny seemed to have little interest in the classification of plant on the basis of their resemblances. He classi

Show gastrointestinal diseases and disorders, Q. Show Gastrointestinal Dise...

Q. Show Gastrointestinal Diseases and Disorders? Before discussing the many gastrointestinal problems, it is useful to understand the basic physiology of the gastrointestinal t

gas exchange, what are the organs of respiration in the lower form of anim...

what are the organs of respiration in the lower form of animals?

Outbreak of staphylococcal food poisoning, Q. What are the conditions favou...

Q. What are the conditions favouring the outbreak of Staphylococcal food poisoning? • Botulism is caused by the bacteria Clostridium botulinum. • All low acid foods can s

How vitamin k used to prevents bone loss, How Vitamin K used to Prevents bo...

How Vitamin K used to Prevents bone loss? Vitamin K is known to inhibit bone loss through inhibiting effect on osteoclast formation. Thus, adequate levels of vitamin K must be

What is the concept of lophophore, What is the concept of Lophophore? A...

What is the concept of Lophophore? A unique double ring of hollow ciliated tentacles that surround the oral opening in some animal phyla. There is some debate as to whether the

Explain the composition of blood agar, Explain the Composition of Blood Aga...

Explain the Composition of Blood Agar? Infusion from beef heart - 500 gm Tryptose - 10.0 gm Sodium Chloride -  5.0 gm Agar - 15 gm Distilled water - 1000  ml pH

What is spermatogenesis in human biology, What is Spermatogenesis in human ...

What is Spermatogenesis in human biology? The male sex cells, or sperm, are produced in the oval-shaped testes, the primary sex organs of the male reproductive system, in a pro

What is expression of the dominant phenotype, If we make a cross of two phe...

If we make a cross of two phenotypically WT zebrafish, and we get the following results, what can we say about the genotype of those two WT looking parents from the following resul

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd