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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is eutrophication" describe its consequences on the life of plants and animals living in such waters. Why is oxygen depletion in a eutrophicated water-body faster at nigh
I need help with p-p, q-q and r-r intervals and heart rates
What is the difference between transcription and translation? Transcription is the name given to the formation of RNA molecules from an open DNA chain used as a template. Trans
Q. Does glycolysis take place within the mitochondria? Glycolysis happens in the cytosol and not in the mitochondria. Pyruvic acid molecules later enter mitochondria to partici
What are the cytokinins? And where are they made? The Cytokinins are phytohormones active in the promotion of cellular division they slow down the aging of tissues and act toge
function of topoisomerace?
Question 1: (a) Describe the maturity stages in fish. (b) "k" and "r" selection strategies are commonly used to describe the response of fish to biotic and abiotic cond
Discuss the example of Comprehensive neuropsychology Example of these include deficits of visuo-spatial perception hampering performance on construction tasks, poor comprehe
Explain the Root amputation, Hemisectioning and Bicuspidization Root amputation: removal of one or more roots of a multirooted tooth "upper teeth". Hemisectioning: surgic
EVOLUTION OF THE CELL All the living organisms are made up of cells. A living cell may be defined as a compartment enclosed by a membrane that consists of several chemical cons
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