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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Phases of Ovarian Cycle In non-mammalian vertebrates the ovarian cycle consists of the following phase; Pre breeding phase Breeding phase and Post breedin
Q. What are the two major divisions of the chordate phylum? Chordates are divided into vertebrates and protochordates (urochordates and cephalochordates). Q. What are the d
Explain about the Anti Obesity Drugs? The anti-obesity drugs can be classified into two broad groups as indicated in the Figure. Drugs must be taken only under Doctor's
Define the Concept of Nutrition? Nutrition may be defined as the science of food and its relationship to health. It is concerned primarily with the past played by nutrients in
conductometry and its uses in biology
Define Regulation of Water Balance? The input of water, as well as, its loss can be highly variable due to individual habits and environmental factors; in spite of this, the to
Q. Causes of oesophagitis? The causes include tissue erosion by hydrocliloric acid (EIC1) and pepsin, with symptoms of substernal burning, cramping, pressure sensation or sever
Effect of Metamorphic Hormones on Gene Expression Effect of metamorphic hormones on gene expression in moulting and metamorphosing insects, the first report of a particular ho
Q. Define Low - density lipoprotein? Ans. LDL is the major cholesterol-rich lipoprotein carrying approximately 70 per cent of plasma cholesterol. It serves to transport ch
It is made up of three cusps, anterior, posterior and septal. The septal cusp is attached to the medial margin. The anterior cusp is attached to the superior-lateral part of the ma
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