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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Why is not Mendel's second law always valid for two or more phenotypical traits of an individual? The Mendel's second law, or the law of the independent assortment, is valid fo
difference between anaerobic respiration and fermentation
Metabolic Significance of HMP Pathway Having gone through the HMP pathway, you would have got some idea about the significance of this alternative oxidative pathway for t
Which organs of the body are part of the human digestive system? The digestive system, also called as "systema digestorium", or gastrointestinal system, is composed of the dige
structure of oxysome
1) What is the effect of increase temperature on a non enzyme catalyzed reaction? 2) What are the effects of increased temperature on an enzyme catalyzed reaction?
The terms ecosystem was introduction by Sir Arthur tensely in 1935. An ecosystem can be defined s " a structural and function unit of biosphere consisting of living organisms and t
Q. What are ganglia? Neural ganglia or Ganglia (singular ganglion) are structures located outside the central nervous system (for instance, beside the spinal column or near vis
Q. What is ST Elevation? Although a stable LV aneurysm may manifest ST-segment elevation in the precordial leads at rest. Some subjects with normal hearts show a degree of elev
how many types of respiration are?define them also
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