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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Causes of Degradation of Ecosystem Due to Agriculture Degradation of the ecosystem due to agriculture can be on the following accounts: a) Removal of trees (deforestation
What key idea, contained in Malthus's essay on populations, helped Darwin formulates his theory of natural selection?
Despite having a great biodiversity why is the Amazon Rainforest under risk of desertification? The natural soil of the Amazon Rainforest is not very fertile but it is enriched
Mechanisms for Response – Protozoan Protozoa are sensitive to many kinds of stimuli like touch, temperature changes, light, chemicals, etc. It is not clear how they do so but
Give the introduction to Congenital Heart Disease ? The past 3 or 4 decades have witnessed a revolution in pediatric cardiac sciences. A number of conditions previously conside
Why is sub-culturing essential in plant tissue culture? Explain the process of heterosis. How is it dissimilar from inbreeding depression? Where and how is urea produced in
Explain about Stacking faults Stacking faults happen in a number of crystal structures, but the common example is in close-packed structures. Face-centered cubic (fcc) struct
Respiration It is important that you comprehend the main points because they ate essential for your understanding of respiratory physiology. To enter or leave the body, oxygen
Stages of Contact Osteogenesis Osteoconduction: This is the first and the most important healing phase and relies on the recruitment and migration of osteogenic cells to the
Proteins of Plant Origin - Nuts Nuts are excellent sources of proteins. Examples of nuts include cashew nuts, almond nuts , hazel nuts , coconuts, walnuts, brazil nuts ,cashew
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