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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define the Obesity in Children? It is difficult to measure overweight or obesity in children and adolescents because they grow and mature at different rates. Weight status in c
1. Northern blotting The quantity of rna species can be quantified by calculating the quantity of rna through northern blotting which provide size and sequence information
Nature of neuropsychological tests Neuropsychological tests are aids in the neuropsychological examination. The tests calculates specified psychological processes including the
what is the classification of skeletal tissue?
i want to extract my compound of interest from tlc i m finding some enzyme inhbitor from microbial source can you plzz suggest me how to prepare nad get my compound
Salmonellosis Salmonellosis is an infection caused by bacterial species belonging to the genus Salmonella and usually associated with the ingestion of food containing salmonel
Q. What are the zymogens? proenzymes, or Zymogens, are enzymes secreted in inactive form. Under some conditions a zymogen shifts to the active form of the enzyme. Zymogen secre
Uses of Plural Embryos Although the basic that trigger causes polyembryony are not fully understood, there has been no dearth of interest in exploiting supernumerary embryos.
How are mineral salts, water and food (sugar) transported throughout the plant? Mineral salts, Water and sugar are transported throughout the plant through conductive vessels f
Q. List the major chemical changes occurring in food? The major chemical changes occurring in food are lipid oxidation leading to rancidity, loss of vitamins and degradation
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