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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is NAD? What is the role of the NAD molecule in glycolysis? NAD (nicotinamide adenine dinucleotide) is a hydrogen acceptor essential as reductant (to receive hydrogen) in
What happens to a denatured enzyme regarding its functionality? How can that result be explained with the help of the lock and key model? According to the lock and key model th
Hom o sapiens neanderthalensis (NEANDERTHA L MAN) - Fossil discovered by C. Fuhlrott (1856) from Neander htat valley of Germany. This resembled modem man, cran
Two compartments of equal volume of physiological saline are divided by a membrane permeable only to oxygen. At 1 AM, equal amounts of oxygen are dissolved into both left and
What are the Results of prosthetic valve endocarditis? The hospital mortality for valve replacement for endocarditis varies between 4- 30 per cent. Operative mortality is highe
Q. What are the hormones secreted by the adenohypophysis? What are their respective functions? The ACTH (adrenocorticotropic hormone), adenohypophisys secretes GH (growth hormo
Proteins Protease inhibitors: Protease inhibitors are protein-based substances widely distributed within the plant kingdom, including the seeds of most cultivated legumes wh
Determine about the microtubules The microtubules are hollow tubular structures formed by a special arrangement of two proteins called the alpha and beta tubulins. The microtu
What are the uses of formycin B? Formycin B is a drug used to demolish of intestinal parasites.
Explain Theory or Principle of Gram Staining of Bacterial Cultures? Foods are rich in various nutrients and can support the growth and survival of microorganisms. The microbial
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