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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. What does this fungus looks like? Ans. The important characteristic features of aflatoxicosis are jaundice, cirrhosis. Long term exposure to aflatoxins could lead to
Indications for Surgery: In a mixed lesion, either regurgitation or stenosis may be dominant and decision of surgery depends on the haemodynarnics. At the time of surgery on othe
Q. Nutrient requirement during angina pectoris? The nutrient requirement here is the same as discussed earlier, however, to sum up it can be said that we need to restrict calor
Candida albicans, and Aspergillus species are the most common of the many fungal organisms identified as causing IE. Fungal endocarditis arises in specific settings. Valve replace
Catecholamines increase activation of Ca 2+ via beta-adrenergic receptors and the adenyl cyclase system. Dopamine has both alpha and beta and dopaminergic effects. Its beta ef
Q. Why is carbon dioxide concentration a limiting factor of the photosynthesis process? And When the carbon dioxide concentration is increased indefinitely is photosynthesis also i
Define beneficial effects of functional foods? Several beneficial effects of functional foods have been reported, which include effects such as antioxidant, anticarcinogenic, b
Under which forms is the Trypanosoma cruzi found in its hosts? In the definitive hosts as well as in triatomine bugs (middle hosts) the protozoan that causes Chagas' disease ch
Blood Pump : Ideal Characteristics 1) I should be able to pump up to seven litres of blood per minute. 2) It should not damage cellular and cellular components of blo
Q. What is ascaris? What is the disease caused by this worm? Ascaris lumbricoides or Ascaris is an animal of the nematode phylum that is a roundworm. Ascaris causes ascariasis,
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