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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
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Alteration of Turgor of Guard Cells There are two ways in which the relative turgor of guard cells may be altered. A decrease in osmotic potential or A decre
is the leukamia dangerous
HISTORICAL DEVELOPMENT OF PSYCHIATRIC NURSING: History is a record of opportunities, recognized and informed, that produce profound changes in a given field. History of psyc
Q. Doppler evaluation for constrictive pericarditis ? 2D Echocardiography and Doppler evaluation can provide valuable clues to the presence of constrictive pericarditis. M-Mo
Why are vaccines used in the prevention but not in the treatment of infections? Why can antivenom serums be used in prevention and treatment? Vaccines are not used in the treat
a) A cat has just arrived to the veterinary clinic with low blood pressure. The cat weighs 5 kg, and as the on call Vet you want to use a bag of saline to prepare a dopamine drip f
Phosphorus deficiency Deficiency of phosphorus is usually primary and is widespread under natural conditions. It is characterized by pica, poor growth, infertility, and osteody
Metallurgical coke should have following characteristics: 1. Purity: low amounts of moisture, ash, sulphur & phosphorous should be present. 2. Porosity: it should b
classification of grantia
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