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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is the excreatory organ in agama lizard
Explain about the Net Protein Ratio (NPR)? This method was introduced by Bender and Doell (1957) and is a modification of the PER method. In this method, an allowance is made f
Q. What is Salty taste? Various ions both cations and anions are responsible for the salty taste. These include: K (Potassium), Na (Sodium), Li (Lithium), Cl, Br (Bromine), I (
What is the logic of the transfusional compatibility concerning the ABO blood group system? The transfusional compatibility for the ABO system takes into account the antigens s
Q. Under which conditions do aerobic cells carry out fermentation? Some cells that usually obtain energy from aerobic cellular respiration can perform fermentation when oxygen
Chromatin is made of DNA molecules associated to proteins known as histones.
When a fatty acid reacts with glycerol, the result is- Select one: a. Formation of an amide b. Formation of an ester c. Formation of hydrocarbon d. Formation of a th
Technique : The suitability of the pulmonary autograft for aortic valve replacement has to be studied by accurate measurement by echocardiogram of the aortic and pulmonary annuli.
How does water move inside sponges? What is the function of the pores in these animals? Sponges are filtering beings. They make water enter their bodies by their lateral pores
RESTRAINING: Several treatment alternatives exist for intervention in assaultive behaviour. Medication, physical restraints and seclusion rooms may be used separately or
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