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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Oedema - Clinical Signs of Kwashiorkor? Oedema: Oedema refers to accumulation of fluid in the tissues and usually begins with a slight swelling in feet gradually spread
Filariasis Filariasis is a chronic infection caused by filarid worms. Around 10 species of Filaridae family are parasitic to man. Adult worms live in the tissues or body cavities
complete definition of Sporogenous tissue
Blotting is the technique for detecting one RNA within the mixture of RNAs (a Northern blot) or one type of the DNA within of a mixture of DNAs (a Southern blot). A blot can prove
Where can RNA be found within cells? In the eukaryote cell nucleus RNA can be found dispersed in the nuclear fluid, with DNA, and as the main constituent of the nucleolus. In c
what is the function of dna primer
Cultured plant cells: Cultured plant cells are recognized to give biochemicals of interest since 1950's , but starting the yields were very low. Refined culture structures hav
Cleavage - Metazoa The unicellular zygote begins cell division (cleavage). First, the single cell divides forming two cells, these redivide further to form four, then eight ce
Q. What are the values of DPD for plant cells under hypertonic, hypotonic and isotonic media? In plant cells under hypertonic medium there is loss of water for the external, SF
Structural Function - Essential Elements The elements nitrogen, phosphorus and sulphur absorbed from the soil are essential components of amino acids and nucleotides. The othe
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