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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the Characteristics of Starch Granule Starch granules, primarily, are made up of amylose (20-30%) and or amylopectin (70-80%) molecules arranged radially. Each granule
Q What is genetic code? Genetic code is the key for the conversion of the DNA nucleotide sequences and thus the RNA nucleotide sequences into amino acids sequences that will co
conductometry and its uses in biological experiments
Doppler echocardiojgraphy measures blood flow velocities and direction of blood flow in the heart add great vessels. The characteristics of blood flow are evaluated using both a
State the term - Neuroanatomists Broadmann Neuroanatomists Broadmann used a combination of staining and microscopy to map the cytoarcitecture (cell structure\type) of human ce
Feeding Mechanisms of Animals All animals have evolved successful methods for extracting their required nutrition from the environment. Thus we find a diversity of feeding mec
Drugs for hepatitis B Chronic HBV infection is currently treated with interferon alfa, lamivudine or adefovir. Lamivudine is much better tolerated than interferon and much les
Give three examples of human disorders which are caused by the action of a single pair of alleles. In every case say whether the harmful allele is dominant or recessive to the non-
CELL COAT (GLYCOCALYX) Present in Protists and animal cells. Usually made of oligosaccharide side chains of glycolipids and glycoproteins. Cell coat has very charged t
A cell with a euploid number of chromosomes contains: A.one half of a complete set of chromosomes B.several complete sets of chromosomes C.the correct number of chromosomes D.two c
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