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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
explain the 4 stages of aerobic respiration
OSTEOPORASIS - Reduction in bone tissue mass causing weakness of skeletal strength. It leads to crush fracture. It occurs in pastmenopausal women & elderly man. Common
Distribution of Stomata There are two parameters normally used for expressing the distribution of stomata. Stomatal frequency: The number of stomata per unit area
Coal can be classified into following categories in the order of rank. Peat: peat is a brown and fibrous mass. It is the first stage of coalification. It is not used as
extensive note on the excretory system of amphibian
chracters
Q. Learning objectives of gastrointestinal disease? • explain the disease conditions, causes, complications of the disorders of the gastrointestinal tract, • discuss the ef
Q. What are sensory receptors? Sensory receptors are structures specialized in the acquiring of information, mechanical pressure, like temperature, pH, luminosity and chemical
Cyanide - Enzyme-activity Control Plants generate cyanide from cyanogenic glucides and histidines. Ethylene biosynthesis is also accompanied by cyanide production. Nitrate red
Q. Hutchinsons System of Classification? John Hutchinson, a renowned British Botanist proposed 24 phyletic dicta or principles and based on these dicta he published a phylogene
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