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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is a genetic family tree? Genetic family tree is a schematic family tree that shows the biological inheritance of some trait by successive generations. Genetic family t
TYPES E.R. consists of two types - 1. Rough E.R. 2. Smooth E.R. R o ugh E.R. S m o o th E.R.
Dietetic Foods for diabetes: With advances in food technology, foods for special use by diabetics are now available in the global market, they are emerging in India also. These
Indications for Surgery : Echocardiography or right heart catheterization can quantify tricuspid stenosis. It is categorized as Tricuspid valve repair is advised when ther
is paper broken down by microorganisms
S i m p l e indigestion The disease is common in stall-fed individuals. Consumption of indigestible food material, moldy and nutritionally unbalanced feed results in simpl
Gutta percha removal: Poorly condensed GP or single cone technique, - Hedstrom hand files. - Barded broaches. The well condenced GP: - Rotary files; - Ultrasoni
Pathophysiology If the ductus arteriosus does not close after birth, the higher pressure in the aorta than in the pulmonary artery causes the blood to flow from the aorta, t
Fast Response - Biological Responses Some of the fast responses are discussed below: It was found that when mung bean root tips were kept in a specific solution (contain
Metabolism Metabolism as you know is a process by which living organisms acquire and use energy. A large number of biochemical reactions take place in the living organisms dur
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