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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Investigation of Tricuspid stenosis by Echocardiography? Findings are subtle. One should carefully search for organic tricuspid valve involvement in all rheumatic heart dise
Hypertensive emergencies should be treated within one hour. Hypertensive urgencies may be treated more slowly. The term accelerated malignant hypertension is used when retinal haem
define alimentary canal
Energy Energy: Fever is characterized by elevation of BMR, thus caloric requirements are increased.. The increase in caloric needs is dependent on severity of infection
Define functions of magnesium - Macro minerals? Like Ca, Mg too has a role in bone formation. Soft tissue magnesium functions as a cofactor of many enzymes involved in energy m
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what are diploblastic organisms?
What is the difference between facultative anaerobic beings and obligate anaerobic beings? Facultative anaerobic beings, such as the fungi Saccharomyces cerevisiae, a brewing y
What is Tagma, Tagmosis and Tagmatization? Distinct body regions resulting when different segments of a metameric animal become involved in specific functions. These segments a
At the starting of the first round of elongation, the initiation codon (AUG) is positioned in the P site with fMet-tRNAfMet bound to it by codon-anticodon base pairing. In The nex
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