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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
A Molecule of DNA is 2.17 um long. The ends of the molecule become strongly ionixed: negative on one end, positive on the other. The helical molecule acts like a spring and compres
Mr. Jones I.V. was hung at noon. It is a liter bag and is infusing at 65 ml/hr. How many hours will this bag last?
Q. What are the two divisions of meiosis and what are the major events that occur in those divisions? Meiosis is divided into first meiotic division, or meiosis I, and second m
Describe the Plasma Homocysteine levels ? Several studies have investigated the contribution of homocysteine to CVD risk both among immigrant Indians and those living in India.
What is Physical Accommodation Physical Accommodation is the actual physical deformation in the lens power. It is measured in diopters, e.g., if the focussing power of the eye
The clinical manifestations of IE result from the local destructive effects of intracardiac infection; the embolization of bland or septic fragments of vegetations to
Antibodies of dissimilar specificities, which react to the same antigen, are known as polyclonal antibodies.
Specify the things needed for a nurse-led primary health care practice relating to childhood obesity. In particular, you need to do the following two things: (a) Indicate:
What are multiple alleles? Is there dominance in multiple alleles? Multiple alleles is the phenomenon in which the similar gene has more than two dissimilar alleles (in normal
Define Factors that effect birth weight and gestational duration? A WHO review has identified 43 factors that influenced birth weight and gestational duration. They are grouped
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