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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is the leaf cuticle? The leaf cuticle is a thin waxy layer made of cutin and waxes on the outer surface of the leaf epidermis. Its function is to control the cellular tran
using an example in each case,describe the three main feeding methods used by animals based on the type of food available
Define two basic principles of food processing? The fundamentals of food processing, as you may recall, involves the following two basic principles: Prepare the products
Signify the term - Pectoral girdle. Bones in vertebrates that connect the appendages on the left and right side of the anterior appendicular skeleton to each other. Pectoral gi
Define Dietary Management of Cancer Patients and Feeding Problems Related To Cancer Therapy? We took an overview about the general nutritional requirements of cancer patients.
Q. Why are transgenics considered a threat to the environmental safety? The Transgenics can be dangerous to the whole biosphere since the transfer of genes between species may
Q. Explain about S-Shaped incision for a mini pedicle flap? The S - shaped incision is indicated where a papilla needs to be developed and was first described by Palacci. This
In genetic recombination by crossing over what is the difference between parental gametes and recombinant gametes? The Parental gametes are those gametes that maintain the orig
Define Procedure for Determination of Blood Glucose by Nelson Somogyi Method? 1. Label a series of Folins tubes with blank (B), standard (S1-S5) and sample (SA 1-2) and place
Process of Memory: There are three stages of memory Encoding process: It is the process of receiving sensory input and transforming it into a form or code, which can be stored.
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