Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

What are the signs of cardiac tamponade, Q. What are the Signs of cardiac t...

Q. What are the Signs of cardiac tamponade? The clinical presentation will be that of a low output state with anxiety, restlessness, dyspnoea, sweating, cold extremities and dr

Normality, what is the normality law?

what is the normality law?

Define essentiality of nitrogen for growth of micro-organism, Define Essent...

Define Essentiality of Nitrogen for Growth of Micro-Organism? Nitrogen is an essential atom in certain cellular macromolecules, e.g. amino acids, purines, pyrimidines, enzymes,

What are retroviruses, What are retroviruses? How do they reproduce and wha...

What are retroviruses? How do they reproduce and what is the role of the enzyme reverse transcriptase? Retroviruses are viruses whose genetic material is RNA. HIV and the virus

What is kingdom monera, What is Kingdom Monera? The eubacteria, archaeb...

What is Kingdom Monera? The eubacteria, archaebacteria, and blue-green bacteria (or cyanobacteria) which form Kingdom Monera lack the cellular organization that all other livin

Neo darwinism or synthetic theory of evolution, NEO DARWINISM OR SYNTHETIC ...

NEO DARWINISM OR SYNTHETIC THEORY OF EVOLUTION - It is the synthesis of the ideas of Neo Darwinians as a new theory of evolution based upon recent findings of genetics and m

Explain the nonpolar molecular attractions, Explain the Nonpolar Molecula...

Explain the Nonpolar Molecular Attractions in basic chemistry? Nonpolar Molecular Attractions :  Van der Waals forces and hydrophobic interactions are weak (not as strong

Animal kingdom, what is animal kingdom and what are its functions

what is animal kingdom and what are its functions

Atp, ATP The energy currency of the body One of the monomers u...

ATP The energy currency of the body One of the monomers used in the synthesis of RNA Contains three phosphate groups àhigh energy Removal of one releases e

Explain cell surface membrane, Diagram of a cell surface membrane.  ...

Diagram of a cell surface membrane.  State the width of a cell surface membrane. (i) On which side of the membrane, shown in Figure, X or Y, is the cytoplasm of the cell?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd