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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What is Radiographic Template Radiographic Template : You should always use it while taking a panoramic radiograph of the patient. It is done to counter the effect of magnific
What is the significance of Pronephric? The first, or ancestral, kidney that appears in the anterior part of the coelomic cavity and is connected to the archinephric duct. In a
Fl u o r osi s Continual ingestion of small amount of fluoride through feed or water leads to fluorosis in animals. The toxic effects are based on amount of fluorine inges
State the Versions of Binocular movements Versions are movements of both eyes in the same direction. a) Dextroversions: Movements of both eyes to'the right side. b) Levov
How different are fecundation in osteichthyes and in chondrichthyes? In chondrichthyes fecundation is internal by means of copulation. In osteichthyes fecundation generally is
Phylum Chordata- Chordata name given by Balfour. Herst put hemichordata into separate phylum. Lamarck gave the word tunicata & vertebrata. Pisces classified by Rom
PEROXISOMES AND GLYOXYSOMES Certain small ovoid unit membrane bound sac like cytoplasmic organelles, resembling lysosomes in size and appearance were isolated from var
Define the Prevention of Adverse Food Reactions? Considering the increasing incidence, cost and morbidity associated with allergic reactions, it is perhaps useful to design pre
In the lack of tryptophan shown in the figure, a trp repressor protein encoded by a split operon, trpR that is synthesized and forms a dimer. Furthermore, this is inactive and so
Examination of the Prosthesis It is important to examine the prosthesis thoroughly. This involves assessing mobility, checking screws, evaluating attachments and porcelain res
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