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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Differentiate between sterilization and disinfection? 1) Sterilization is defined as freeing the object or substance from all life of any kind. It is the process by which a
Nitrogen Control of Nitrogen Assimilation N 2 -fixer like Klebsiella pneumoniae and Nostoc can grow with N 2 , NO - 3 or NH + 4 as nitrogen source. You would like to know
Q. How many chromosomes does a human normal diploid cell have? How many chromosomes does a human normal haploid cell have? How many are the sex chromosomes within each of them?
Explain Irradiation The physical process of exposing an object, system, or a material to a high radiant energy for the sterilization or preservation.
What is the activity of a 0.35 M/L CaCl2 solution? What is the osmolarity of this solution?
How can germs can be transmitted Germs can be transmitted many ways, including: Touching dirty hands; Changing dirty or contaminated cloths; Through contaminated water and f
Nature: Matter and Energy Nature is not just our immediate surrounding or environment, but is the whole Universe of Cosmos. Study of the origin and topography of cosmos is ca
Explain Fluids Fluids: Fluid diets are given to patients with more advanced dysphasia or fractured jaws. The diet may include fruit juices, thin strained porridge with milk, e
Can someone explain how is cancer related to ras and p53 gene? I know that the ras protein regulate cell growth and its mutation will cause cancer. How is the mutation caused?
Dysrhythmias Usually occur during the initial 24-72 hours, but may occur later on also. The causes may be ventricular irritability due to manipulations of heart durin
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