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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Biochemical Function - Essential Elements Elements Mg, Mn, K, Ca and Fe are cofactors for many enzymatic reactions. Fe is carrier of electrons in electron transfer chain. Phos
Examples of cloning and molecular biology cloning may produce heterozygous individuals (as mother was heterozygous) and suggests a way to get pure breeding cats molecu
Q. What is hypercholeslerolaemia? Cholesterol: It is a natural component of foods such as mutton, pork, ham, sausages, lamb, chicken, eggs (yellow), whole milk, cheese, ice-cre
Illustrate the Applications of Gellan gum Gellan is a highly versatile food ingredient and has numerous applications in foods. The combination of gelatin with gellan, by compar
Q. How is the excretory system of molluscs characterized? Molluscs have one or two pair of spongelike nephridia, similar to kidneys
P o lymerase chain reaction (PCR): The PCR technique enables the exponential amplification of nucleic acid sequences from any biological samples. In the last few ye
Define influence of polyphenols on Carbohydrates? Binding of proteins may indirectly affect carbohydrate absorption. Inhibition of amylolytic enzymes and subsequent reduction o
Q. Spoilage of butter? Butter, which has a high content of fat, is subject to rancidity and microbial spoilage due to contaminated cream from which it has been prepared
A home-made wormery A wooden box 30 cm by 30 cm by 15 cm fitted with a glass front is beneficial for studying the habits of earth-worms. Fill the box nearly to the top with
types of conservation
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