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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
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Define the Precursor of co-enzymes - Functions of riboflavin? The major function of riboflavin is to serve as the precursor of the coenzymes FMN and FAD and of the covalently
What is dry mass? When biomasses are compared often the method of dry mass is used. The dry mass is the total mass less the water mass of an individual. The total mass is also
Comparing toxicity and the requirement for dilution in water how different are the ureotelic and the uricotelic excretions? What are some examples of animals that present these res
Cannulation : Typically blood is drained by gravity through two cannulae inserted into the superior and inferior vena cavae. During bypass, if the SVC and IVC are snared, the ent
What concentrations of succrose gradient are used to separate between the two types of endoplasmic reticulum please?
write breif notes on oogenesis
classification and characteristics
Pulse wave transducer has one Doppler crystal. This crystal emits a short burst of ultrasound at a certain frequency [PULSE REPETITION FREQUENCY (PRF)]. The ultrasqund is reflec
Biological Significance of Incompatibility Both interspecific and intraspecific incompatibility determines the degree of inbreeding and out breeding of plants. Self-incompati
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