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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
WHAT IS THE TYPICAL REPRODUCTION CYCLE OF A DNA VIRUS
ADRENAL OR SUPRARENAL GLANDS (GLANDS OF EMERGENCY) - These are paired structures located on the top of the kidneys. Each adrenal gland has two parts external adrenal cort
Q. Explain about Utility Gloves? Because of the increased risk of percutaneous injury during instrument cleaning (100% of the Faculty incidents reported in academic year 2003/0
What are living and nonliving reservoirs? Viruses are both living as well as non-living. They have reservoirs of genes. A one nucleotide is a unit of gene. Viral genes make use
You are working with plants that have either hairy (dominant) or 'bald' (recessive) flowers. Hairy flowers have little hairs on them that make the flowers look fuzzy, bald flowers
What is difference between self pollination and cross pollination? Which of the two modes of pollination contributes more to the plant diversity? Self pollination take places w
Explain about Diabetes mellitus Diabetes mellitus (DM) has relation with all the systems of our body. With some systems it has direct relationship, while with others it has in
When did life appear on earth? It is estimated that the life on earth emerged about 3.5 billion years ago, thus 1 billion years after the formation of the planet.
Isomer : Existence of different compounds having same molecular form but different structural forms.
What is inflammation? Inflammation is the initial response of the unspecific defense system versus aggressions against the body (the aggressions might be caused by infectious p
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