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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Show Upsloping ST-Segment? There are few subjects in exercise literature that has generated more controversy than the significance of up sloping ST-segments. The up sloping
What is dry mass? When biomasses are compared often the method of dry mass is used. The dry mass is the total mass less the water mass of an individual. The total mass is also
what is the differnce between syndrome and symptom?
wt is an air bladder and its function wt is catadromous and anadromus migration
What is ADP phosphorylation? What respectively are photophosphorylation and oxidative phosphorylation? ADP phosphorylation is the addition of one inorganic phosphate in the mol
Explain Starch - Tests for the Presence of Exoenzymatic Activity? Starch is a polymer of glucose linked together by glycosidic bonds. Microorganisms produce hydrolytic enzyme a
Causes of Air Pollution We known about the composition of atmosphere, its major and minor constituents. The composition of atmosphere has remained the same for thousands of y
ATG or AUG: The codon for the methionine; the translation initiation codon. generally, protein translation can only start at the methionine codon (although this codon might be fou
Q. Waste produced during surgical process? Regular garbage: Regular garbage generated from clerical office procedures or kitchen waste should be disposed of within the usu
What are the three major groups into which mammals are divided? The three groups into which mammals are divided are: monotremes (or prototherian, e.g., platypus), marsupials (o
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