Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Role of exercise and drugs in management of diabetes, Q. Role of Exercise a...

Q. Role of Exercise and Drugs in management of diabetes? Aerobic exercise for at least 20-30 minutes four or more times a week is recommended. Exercise after meals is preferre

Chromosomes, Chromosomes  are the condensed, fibrillar, self- replicating g...

Chromosomes  are the condensed, fibrillar, self- replicating genetic structures of cells containing the cellular DNA which bears in its nucleotide sequence the linear array of gene

Humans which distinguish them from other primates, State two characteristic...

State two characteristics of humans which distinguish them from other primates. Two features which differentiate humans from other primates are their upright posture and their

What is the classification that divides orders, What is the classification ...

What is the classification that divides orders? Orders are divided into Families. The hierarchy of classification of living things most usually used is, from broadest to nar

Explain about the physiological accommodation, Explain about the Physiologi...

Explain about the Physiological Accommodation Physiological Accommodation contractile power of the ciliary muscle required to change the focussing power of the eye by ID. Meas

What do you mean by respiratory pigments, Q. What are respiratory pigments?...

Q. What are respiratory pigments? What are some respiratory pigments and in which animal groups can each of them be found? Respiratory pigments are molecules present in the blo

Define the sources of vitamin d, Define the Sources of Vitamin D? Vitam...

Define the Sources of Vitamin D? Vitamin D, also called the sunshine vitamin is easily manufactured in the skin from 7-dehydro cholesterol on exposure to sunlight, as already m

Photosynthesis, in a school laboratory, what is usually regarded as evidenc...

in a school laboratory, what is usually regarded as evidence that photosynthesis has occurred in plants

Swine-pox, Swine-pox Pigs of 2 months of age may be infected with vaccinia...

Swine-pox Pigs of 2 months of age may be infected with vaccinia either naturally or artificially and show pox lesions on the eyelids, snot, inside the thigh and undersurface of th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd