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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define the effects of Deficiency of Calcium in the Body? If there is a continued inadequate intake or poor intestinal absorption of calcium, plasma calcium concentrations will
Phylum Zygomycetes 1) Asexual reproduction by non-motile spores produced from a stalked spore case which is known as sporangium. 2) They reproduced by- conjugation between n
A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction
Define the Precursor of co-enzymes - Functions of riboflavin? The major function of riboflavin is to serve as the precursor of the coenzymes FMN and FAD and of the covalently
Q. Explain Limited mucosal contact diarrhoea? Limited mucosal contact diarrhoea: It results from situations of inadequate mixing of chyme (semi-liquid mass of food passing thro
Determine the parts consists in Our urinary system Our urinary system consists of following: Two kidneys Two ureters One urinary bladder One urethra Apart fr
What are some examples of biological activities in which osmosis plays an important role? Hemolysis (destruction of red blood cells) by entrance of water, the hydric regulation
Poxvirus zoonoses Naturally occurring poxvirus infections affect humans and many species of animals and insects. However, for most part, poxviruses other than variola cause self-l
Jackson Coleman and Fischer Theory Jackson Coleman Theory: During accommodation the ciliary muscle contraction causes an anterior thrust of the vitreous. This vitreous thrust p
a) Giving two reasons define why there is more species biodiversity in tropical latitudes than in temperate ones. b) Name an upload drug and its source plant. How does the dr
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