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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define Primary Level Care - Public Nutrition? This is the first level of contact of an individual, the family and the community with the national health system. It is possible
A large leaf on a plant in bright sunshine evaporates 5 grams of water per hour. Cooling the leaf through evaporation in this manner enables the plant to eliminate heat energy at t
why do ecological models commonly have limited applications?
Explain Atrial Switch Operation ? The hospital mortality reported varies between 0 and 15 per cent. Late survival is worse for TGA with VSD compared to simple TGA. 15 year surv
Q. Clinical Features of gout disease? You may be enjoying good heath but may suddenly get a severe attack. You wake up in the middle of the night, and your big toe feels as if
Individual components of biodiversity provide an unbeliev able wide range of products that are used to enhance the lives of people in all countries of the world. It is the diversit
Explain Results of abdominal aneurysm surgery? Results: For ascending aorta replacement, the hospital mortality varies from 0 to 9 per cent. Aortic arch has higher risk of
Explain Oxygen Therapyin respiratory support? Oxygen is given to correct hypoxemia. An initial high concentration of oxygen (FiO2) is given while the underlying cause is identi
Q. What happens when the oxygen supply is inadequate to maintain aerobic cellular respiration during muscle exercise? If oxygen from myoglobin or hemoglobin is not enough for t
How much SDS do you need for 50 ml of a 20% SDS solution in water(weight/ volume)? SDS is a solid.
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