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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
By-Products from Sugar Industry India is one of the important sugarcane (Saccharum officinarum) growing countries of the world. Five main by-products are available from the su
Q. Fats requirement in chronic diarrhoea? Total amount of fat may be restricted as its digestion and absorption is compromised. In order to increase on the calorie density of t
how chlorophyll is degaded in plants?
Q. How different are the concepts of action potential, resting potential and excitation threshold concerning neurons? Action potential is the maximum positive voltage level ach
Q. Why does not a long lasting vaccine against common cold exist yet? Viruses that present a high mutation rate like the virus that causes the common cold escape easily from th
STRIPED MUSCLES - These muscles are found in the limbs, body walls, tongue, pharynx and beginning of oesophagus and are under the control of animal's will. These muscle f
Dictyosomes are produced on the Organelles in the plant cells composed of the series of flattened membrane sacs which sort, chemically modify, and package protein srough endoplasm
Dormant Vegetative Structures You have learnt that light a period of chilling or application of gibberellin often breaks seed dormancy and stimulates seed germination. Vegetat
Sucrose is commonly used to preserve fruits why not glucose? Ans) Glucose is reactive due to its open-chain form having an aldehyde group.
Composed of C, H and O (fewer polar OH bonds than carbohydrates) Can be used at energy storage, components of cell membranes, chemical signals & thermal insulation Are
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