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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What raw materials require for the manufacture of margarine Soybean and peanut (or groundnut) oils are of great economic importance. Refined soybean oil contains branched fura
What is the binding between two amino acids called? The chemical bond among two amino acids is called a peptide bond. Protein Structure Review - Image Diversity: peptide bo
Plant sources Although there is a multitude of colours in the plant kingdom, their extraction and use in food systems is not an easy task. Unless the colourants have some outst
Define Nutritional and metabolic factors that contribute to the malnutrition of CHD? Nutritional and metabolic factors that contribute to the malnutrition of CHD infant are ele
Estimate the Results of Tetralogy of Fallot? In excellent centres, the mortality is reported one per cent or less. In infants and babies with low weight, higher mortality is ex
Explain Bioluminescence - Ethological Approach in Taxonomy In those animals which exhibit bioluminescence. The pattern produced by the bioluminescence is used in taxonomy. It h
What are the Advanced surgical procedures The scope of implantology has widened to include many more clinical situations which would be hitherto contraindicated for implants du
Ecosystem Degradation and Wildlife Man is an animal with ecological requirements just like any other animal. It constantly needs a source of energy and.minera1 nutrients for i
Proto-oncogenes Proto-oncogenes are common genes present. In all organisms and emerge to be highly conserved in the course of evolution. They have been isolated in species ra
Psychology : This is the study of human mind. On other words Psychology is the science of behavior or mental processes. Its immediate aim is to understand the individuals or groups
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