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Explain what is Argininosuccinate Synthetase
In a 2-step reaction catalyzed by the cytosolic enzyme argininosuccinate synthetase, citrulline and aspartate are condensed to form argininosuccinate. This reaction involves the addition ofAMP (from ATP) to the amido carbonyl of citrulline, forming an activated intermediate on the enzyme surface (AMP-citrulline), and the subsequent addition of aspartate to form argininosuccinate as shown herewith.
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