Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Zoology, general characteristics of phylum chordata

general characteristics of phylum chordata

What do you know about cardiothoracic ratio, Q. What do you know about Card...

Q. What do you know about Cardiothoracic ratio? The cardiothoracic ratio (CTR) shown in figure is the transverse cardiac diameter (the horizontal distance between the most righ

Consciousness as inner state, Q. Explain Consciousness as inner state? ...

Q. Explain Consciousness as inner state? Consciousness as inner state: We are conscious of thoughts, images, emotions and memories within ourselves though they may not have phy

Respiration, Define respiration in animals

Define respiration in animals

Gene vs mrna , Obtain the sequence for the gene and the sequence for the RN...

Obtain the sequence for the gene and the sequence for the RNA transcript in FASTA format. If there is more than one transcript choose and appropriate one and explain your choice. U

What are the extraembryonic membranes present in vertebrates, What are the ...

What are the extraembryonic membranes present in vertebrates? The extraembryonic membranes that might be present in vertebrates are the yolk sac, the amnion, the chorion, the

Chromosomes/genes/heredidty, Plot the amount of DNA in the nucleus of a spe...

Plot the amount of DNA in the nucleus of a spermatogonia from the G1 stage prior to the first meiotic division through the completion of meiosis. Label each of the major stages of

Lan in hospitals, LAN LAN stands for Local Area Network. This kind of ...

LAN LAN stands for Local Area Network. This kind of network consists of a set of interconnected computers that are not geographically spread out over a large area. For exa

Phylum coelenterata, what is the food getting habits of phylum coelenterata...

what is the food getting habits of phylum coelenterata?

Polar bear is adapted to its environment, Give three ways in which a polar ...

Give three ways in which a polar bear is adapted to its environment. A polar bear is adapted to its environment by its high ratio of bulk to surface area, a thick water-repelle

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd