Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

How to obtain the correct healing collar, To obtain the correct Healing Col...

To obtain the correct Healing Collar the following steps need to be followed: - Determine the si-e of the implant platform.76 Practical Manual - Select the emergence profile

How is the nervous system of nematodes organized, Q. How is the nervous sys...

Q. How is the nervous system of nematodes organized? Where are the neural chords located in their body? Roundworms have a ganglial nervous system with an anterior neural ring r

What is fenugreek seeds, Q. What is Fenugreek seeds? Fenugreek seeds: '...

Q. What is Fenugreek seeds? Fenugreek seeds: 'Commonly known as methi seeds in Hindi. They are commonly used in Indian cuisine in chutney and even in pickles and several dishes

Lateral and the apical buds of the plants, What is the dissimilarity betwee...

What is the dissimilarity between the lateral and the apical buds of the plants? The Lateral buds are portions of meristematic tissue located in the base of the shoots. The Api

What is exudative diarrhoea, Q. What is Exudative diarrhoea? Exudative ...

Q. What is Exudative diarrhoea? Exudative diarrhoea: It is associated with the mucosal damage leading to out pouring of mucus, blood and plasma proteins with a net accumulation

What are ions, What are ions? What are the two types of molecules into whic...

What are ions? What are the two types of molecules into which ions are classified? Ions are atoms or substances electrically charged by means of loss or gain of electrons.

Budding, What processes occur during the budding of yeast?

What processes occur during the budding of yeast?

Assignment., what are the sub- headings in energy?describe energy in biolog...

what are the sub- headings in energy?describe energy in biology?

What is biodiverse ecosystems, What are some economic applications that can...

What are some economic applications that can be generated by very biodiverse ecosystems? Very biodiverse areas show enormous economic potential. They can be a source of raw mat

Explain erythema migrans, Erythema migrans Oral antibiotic therapy sho...

Erythema migrans Oral antibiotic therapy shortens the duration of the rash and generally prevents development of late sequelae. Among the 3 drugs used for this indication, onl

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd