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Explain Transfer from Solid Culture to Liquid Media
The steps involved in this technique are included herewith:
1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.
2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.
After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth. For liquid culture, "shake water baths" can be used at appropriate constant temperature.
Disease Y Study: Let us consider the Disease Y study, a brief description of the study is as follows. A case-control study was conducted of 75 cases with a rare genetic disorder "
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