Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

What is catalyzes a substrate level phosphorylation, The enzyme in the TCA ...

The enzyme in the TCA cycle that catalyzes a substrate level phosphorylation is -citrate dehydrogenase -succinate thiokinase -isocitrate dehydrogenase

What is the auditory tube and its function, Q. What is the auditory tube an...

Q. What is the auditory tube and its function? Auditory tube (Eustachian tube) connects each middle ear to the throat. This tube conducts air between tympanic cavity and outsid

Disorder of adrenal function, Disorder  of Adrenal Function: The diso...

Disorder  of Adrenal Function: The disorders of  adrenal function may lead  to acute and chronic  .adrenocortical insufficiency. Let  us briefly  describe each  one as follows

Prevention and community action - psychoactive substance, Prevention and Co...

Prevention and Community Action: A number of  preventive action are necessary. Alcohol prevention programmes can have major effects on alcohol problem rates, and can influence

What are ganglia, What are ganglia? Ganglia (singular ganglion), or neu...

What are ganglia? Ganglia (singular ganglion), or neural ganglia, are structures located outside the central nervous system (for instance, beside the spinal column or near visc

Natural disasters, EARTHQUAKES          An earthquake with its terrible...

EARTHQUAKES          An earthquake with its terrible aftereffects is one of the most frightening and destructive phenomena of nature. It can be defined as a sudden movement of

Parathyroid disorders, PARATHYROID DISORDERS - (i) Hypoparathyroidism ...

PARATHYROID DISORDERS - (i) Hypoparathyroidism (deficiency of PTH) . It causes the lowering of blood calcium level. This increases the excitability of nerves and muscles, caus

Explain increases-decreases or shifts in demand terminology, Explain about ...

Explain about the increases or decreases or shifts in demand terminology. Market into equilibrium P 1 Q 1 , here demand D 1 equals supply S 1 • When price reduce fromP 1

Serendipidity, SERENDIPIDIT Y - Sometimes discoveries are made unexpec...

SERENDIPIDIT Y - Sometimes discoveries are made unexpectedly matter of chance. This is serendipidity. Examples - 1.       Structural formula of benzene - It was dreamt by

Mycotic diseases-sheep, Goat and sheep M. mycoides subsp. capri is the...

Goat and sheep M. mycoides subsp. capri is the cause of caprine pleuropneumonia, a serious malady of goats. The disease is prevalent in certain parts of this country. The mort

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd