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Explain Transfer from Solid Culture to Liquid Media
The steps involved in this technique are included herewith:
1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.
2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.
After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth. For liquid culture, "shake water baths" can be used at appropriate constant temperature.
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Put the following in the most probable order
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