Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Endosperm with micropylar haustorium, Endosperm with micropylar Haustorium ...

Endosperm with micropylar Haustorium A very prominent and aggressive micropylar haustorium is seen in Impatiens. Here the division of the primary endosperm nucleus is followed

What proportion of children with down syndrome, What proportion of children...

What proportion of children with Down syndrome do you expect when women with down syndrome have children with men who have 46 chromosomes Justify your answer

Illustrate pathology, Q. Illustrate Pathology? Following rheumatic feve...

Q. Illustrate Pathology? Following rheumatic fever, over next few years to decades, the typical funnel shaped mitral valve assumes a fish mouth appearance. During rheumatic fev

Nerves found in human body, Motor nerves : Nerves which carry impulses fro...

Motor nerves : Nerves which carry impulses from brain or spinal cord to effector organs are called efferent or motor nerves. Stimulation of motor nerves makes the muscles to contr

Fragmentation - types of asexual reproduction, Fragmentation - Types of Ase...

Fragmentation - Types of Asexual Reproduction Fragmentation is a phenomenon where parent animal spontaneously (on its own accord) splits into two or more fragments. Each one o

What are uses of hydrocolloids for health, Uses of Hydrocolloidsfor health ...

Uses of Hydrocolloidsfor health Hydrocolloids, together with other dietary fibres are increasingly being seen as contributing to a number of health effects. Some of the hydroco

Determine some common micronutrient deficiencies, Determine some Common Mic...

Determine some Common Micronutrient Deficiencies? Vitamin A deficiency Iron deficiency anaemia Iodine deficiency disorders Zinc deficiency

Placenta, PLACENTA - A common tissue of foetus & mother (uterus) which ...

PLACENTA - A common tissue of foetus & mother (uterus) which is physical, physiological & endocrinal connection is known as placenta. FUNCTIO N - To provide nutrients

Explain the term- ecology, What is Ecology? Ecology is the field of Bio...

What is Ecology? Ecology is the field of Biology that studies the relationship among living beings and among living beings and the environment.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd