Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Observation or inference for half and full saturation test, Define Observat...

Define Observation or Inference for half and full saturation Test? 1. Starch is precipitated by half saturation with ammonium sulfate whereas dextrin is not precipitated. This

Classical swine fever (hog cholera), Classical swine fever (hog cholera) ...

Classical swine fever (hog cholera) Swine fever, known as hog cholera or European Swine Fever is a highly infectious virus disease characterized by rapid spread and high morbi

Define pipette - nutritional biochemistry, Define Pipette - Nutritional Bio...

Define Pipette - Nutritional Biochemistry? Pipettes are used to deliver a known volume of liquid from one container to another. They are precision instruments of measurement an

Glucose-6-phosphate to glucose, Glucose-6-phosphate to Glucose Glucose...

Glucose-6-phosphate to Glucose Glucose-6-phosphate to Glucose : Glucose-6-phosphate  is converted to glucose  by glucose-6-phosphatase which is present in intestine, liver and

Explain corrective surgery - endodontic surgery, Explain Corrective Surgery...

Explain Corrective Surgery - Endodontic Surgery perforation repair periodontal management (root resection, tooth resection) intentional reimplantation

Sunlight used for sterilization, Q. Sunlight used for sterilization? It...

Q. Sunlight used for sterilization? It possesses appreciable bactericidal activity under natural condition. The action is primarily due to content of the ultraviolet rays; howe

What is meant by the peripheral nervous system, Q. What is meant by the per...

Q. What is meant by the peripheral nervous system (PNS)? The peripheral nervous system comprehends the ganglia and nerves of the body.

Strawberry slain - common disorders of skin, Strawberry Slain: It  sig...

Strawberry Slain: It  signfies the dilatation  of deeper vessels  to form  cavernous spaces like those seen  in  the erectile tissue of the penis. The  lesion  is well-defined

Mechanisms of speciation, In the previous section we discussed the concept ...

In the previous section we discussed the concept of species in detail and concluded that the concept of biological species and Mayr's definition of species have a wide acceptance a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd