Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Explain the fructo oligosaccharides (fos), Explain the Fructo Oligosacchari...

Explain the Fructo Oligosaccharides (FOS)? FOS are polymers of fructose, usually attached to an initial glucose molecule. The total number of fructose units range from 2 to 8 a

Personal protective equipment, Personal Protective Equipment These eq...

Personal Protective Equipment These equipments are essentially required during working to protect face, eyes, respiratory system hands and clothes. Additionally in various in

Structure of vertebrate skeletal muscles, Normal 0 false fals...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Is it necessary for blood to have less or more hemoglobin, Q. In high altit...

Q. In high altitudes is it necessary for the blood to have less or more hemoglobin? In high altitudes the oxygen concentration and air is rarefied is lower than in low altitude

Electrodialysis, This method is used for the desalination of water i.e., fo...

This method is used for the desalination of water i.e., for the removal of dissolved mineral salts, acids, alkalis and also the radioactive substances from the effluents. Electro d

Consequences of aging, Consequences of Aging It is hard to enumerate ...

Consequences of Aging It is hard to enumerate the general consequences of aging in different animals. Gradual loss of reproductive ability, neuromuscular coordination and not

Cell theory, why is virus an exception of cell theory

why is virus an exception of cell theory

Medical management of chronic renal failure, Medical Management   In ch...

Medical Management   In chronic  renal failure  there  is irreversible renal failure. The goals of medical management are:   To promote maximal  renal  function  To ma

Venipuncture - blood collection, Venipuncture : patient shoul...

Venipuncture : patient should be seated or supine for at least 20 minutes before sampling. An arm with an inserted intravenous line should be avoided. The median cubi

Primitive nervous system - nerve net, Primitive Nervous System - Nerve Net ...

Primitive Nervous System - Nerve Net A nervous system creates its appearance for the first time in the phylogeny among Cnidaria. In this group the nerve cells make an irregula

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd