Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Define the iodine test - carbohydrates, Define the Iodine Test - Carbohydra...

Define the Iodine Test - Carbohydrates? This test is given by polysaccharides and is not given by monosaccharides or disaccharides. Principle Iodine forms a coloured ad

Phloem, why phloem made of living cells

why phloem made of living cells

What is the senses explain their vision , What is the Senses explain their ...

What is the Senses explain their Vision ? Vision :  The human eye consists of structures which evolved together to focus light on a sensitive area called the retina. Light f

Aquatic ecosystem - types of ecosystem, Aquatic Ecosystem - Types of Ecosys...

Aquatic Ecosystem - Types of Ecosystem Global waters cover about three quarters of the earth's surface either as fresh water where salt content is less than 0.5 per cent or as

Light requirement - seed dormancy, Light Requirement - Seed Dormancy T...

Light Requirement - Seed Dormancy The light requirement for germination of many seeds is presumably a mechanism that prevents the germination of small seeds buried deep underg

What hormones participate in digestive secretions, Q. The releasing of dige...

Q. The releasing of digestive secretions is controlled by hormones. What are the hormones that participate in this regulation? The hormones that participate in the regulation o

What are the consequences of pem, What are the Consequences of PEM? Th...

What are the Consequences of PEM? The consequences of PEM are most often long lasting and irreversible. The common consequences include: • Irreversible growth retardation

Pentose phosphate pathway, The pentose phosphate pathway also called as the...

The pentose phosphate pathway also called as the phosphogluconate pathway and the hexose monophosphate shunt is a procedure which gives pentoses 5-carbon sugars and NADPH. There ar

Describe the physiology of atrial septal defect, Describe the Physiology of...

Describe the Physiology of atrial septal defect? In ASD the magnitude of left to right shunt is determined by the size of the defect and the relative compliance of the right ve

Embryo and endosperm, What is the difference between embryo and endosperm?

What is the difference between embryo and endosperm?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd