Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

How poor selection of food causing the underweight, How Poor Selection of F...

How Poor Selection of Food causing the Underweight? Poor Selection of Food: Poor selection of food along with irregular eating habits may be responsible for insufficient food

Do we know what causes mental illnesses or how to treat them, Do we know wh...

Do we know what causes mental illnesses, or how best to treat them? Mental illnesses take various forms: debilitating sadness in depression; uncontrollable repetitive actions i

Differences between continuous traits and discrete traits, Can you please d...

Can you please describe the differences between continuous traits and discrete traits and how it helps an ecologist measure variation? Please give a few examples.

How the body gets rid of dust which enters the lungs, Using the words 'cili...

Using the words 'cilia' and 'mucus', describe, very briefly, how the body gets rid of dust which enters the lungs. The lining of the air passages makes mucus which traps dust p

Where in these kinds of cells can dna are found, Q. Bacteria are prokaryoti...

Q. Bacteria are prokaryotic cells, i.e., they don't have a membrane-delimited nucleus. Eukaryotes have cells with a delimited nucleus. Where in these kinds of cells can DNA are fou

Explain pro-oxidants - lipid oxidation, Explain Pro-Oxidants Transitio...

Explain Pro-Oxidants Transition metals, particularly those possessing two or more valency states and a suitable oxidation - reduction potential between them (e.g., cobalt, cop

Intermediate filaments - role of cytoskeleton structures, Intermediate fila...

Intermediate filaments - Role of Cytoskeleton Structures These filaments are intermediate in size among microtubules and microfilaments and are 10 nm in diameter. Five classes

Nucleic acids, Why is the number of bonds important in nucleic acids

Why is the number of bonds important in nucleic acids

How many are the known amino acids, How many are the known amino acids that...

How many are the known amino acids that form proteins in living beings? There are twenty dissimilar known amino acids that form proteins related to the genetic code of the livi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd