Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Define the activation of an agonist during movement, Explain the relationsh...

Explain the relationship between an agonist muscle and its antagonist as it relates to positional control, functional movement and neuromuscular activation. For example, how the th

What is the etiological agent of amebiasis, What is the etiological agent o...

What is the etiological agent of amebiasis? How is it transmitted and what are the typical manifestations of the disease? Amebiasis is caused by the protozoan Entamoeba histoly

Explain about cloning, What is Cloning Cloning: Somatic (ie 2N) cell fr...

What is Cloning Cloning: Somatic (ie 2N) cell from original female cat removed (nucleus has mutated allele), fertilised egg extracted from another cat, nucleus removed, and egg

Define proteins as enzymes, Define Proteins as Enzymes? From conception...

Define Proteins as Enzymes? From conception to death, living cells use oxygen and metabolize fuel. Cells synthesize new products, degrade others, and generally are in a state o

Which is better to grow plants in rock sand or soil, Which is better to gro...

Which is better to grow plants in Rock sand or soil? When we grew plants inside, with there is no wind, and the plants in the rocks grew improved than the plants that were grow

How do you verify that a certain gene encodes a certain prot, For homework,...

For homework, I had to prove that a certain catalyst was a protein, then tell how I would purify the protein, then tell how I would clone a human chromosomal region that contains t

Define the term- organismal complexity, Which of the following statements l...

Which of the following statements least accurately explains our knowledge of how gene number relates to "organismal complexity"? A. Based on known gene numbers there appears to

To study the structure of a seed, To study the structure of a seed Soak...

To study the structure of a seed Soak seeds of bean, pea, pumpkin, sunflower, corn and other large forms. Take away the seed- coats and carefully cut the seeds open. Discover t

What is reverse cholesterol transport, Q. What is Reverse Cholesterol Trans...

Q. What is Reverse Cholesterol Transport? HDL is involved in reverse cholesterol transport whereby cholesterol surplus to cellular requirements is returned from the periphery t

What are homologous chromosomes, What are homologous chromosomes? Which are...

What are homologous chromosomes? Which are the human cells that do not have homologous chromosomes? Chromosomes have genes (genetic information in the form of nucleotide sequen

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd