Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Statements concerning the inheritance of a dominant trait, Which of the fol...

Which of the following statements concerning the inheritance of a dominant trait is true? Every affected person must have at least one affected parent. The trait is observed to ski

Determine the membrane voltage of neuron b, Consider Neuron B in the frog c...

Consider Neuron B in the frog central nervous system whose plasma membrane has a previously unknown channel that is selectively conductive to a newly discovered tetravalent anion n

Trihybrid cross between two true-breeding homozygous, Which of the followin...

Which of the following statements is true regarding a trihybrid cross between two true-breeding homozygous individuals with contrasting phenotypes? Which of the following statement

Explain herbals of brunfels, Explain Herbals of Brunfels? Between the y...

Explain Herbals of Brunfels? Between the years 1530 and 1536 Otto Brunfelsius (Brunfels) (1463-1534) published his 'Herbal' which consisted of descriptions of a large number of

Natality rate - natality, Natality Rate - Natality Natality rate or bi...

Natality Rate - Natality Natality rate or birth rate is determined by dividing the number of individuals born by unit time and is expressed as follows: Natality rate =  ΔNn

Coniferous forests and taiga, As you travel south from the tundra you will ...

As you travel south from the tundra you will enter the circumpolar belt of coniferous forests which stretches across North America to Eurasia, this region is called taiga, a world

Mature embryo, Mature Embryo A typical dicotyledonous embryo, as seen ...

Mature Embryo A typical dicotyledonous embryo, as seen in a median longitudinal section, consists of an embryonal axis having two broad cotyledons. The portion of embryonal ax

Describe assist control ventilation, Describe Assist Control Ventilation (A...

Describe Assist Control Ventilation (ACV) The machine delivers a set tidal volume at the set rate; besides, each time the patient breathes on his own, a machine breath is init

What are the chemical bonds of the dna molecule, Q. What are the chemical b...

Q. What are the chemical bonds of the DNA molecule that are broken for replication process to occur? During the DNA replication process, hydrogen bonds between the nitrogen- co

Which gas expect to be more soluble in water, Which gas in each of the foll...

Which gas in each of the following pairs would you expect to be more soluble in water? Why? (a) Oxygen and Carbon Dioxide (b) Nitrogen and Ammonia (c) Methane and Hydrogen Sulfide.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd