Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

What is oxygen-haemoglobin dissociation curve, What is oxygen-haemoglobin d...

What is oxygen-haemoglobin dissociation curve? Explain the role of red blood cells in the transport of oxygen and carbon dioxide by blood. Briefly describe the principle, pr

Bacteria anerobic respiration, how does the bacteria clostrium tetani respi...

how does the bacteria clostrium tetani respire anerobically

Locomotion in mollusca, Locomotion in Mollusca The major locomotor or...

Locomotion in Mollusca The major locomotor organ in Mollusca is the foot, which is a characteristic feature of these animals. In its simplest form the foot is a flit ventral

Define the sun and solar drying method, Define the Sun and Solar Drying Met...

Define the Sun and Solar Drying Method? The make use of solar energy to preserve the food items is mainly the oldest form of food reservation and is still practiced today for f

Measures of evenness, Measures of Evenness The maximum Shannon-Wiener i...

Measures of Evenness The maximum Shannon-Wiener index for a given number of species can be calculated as: The minimum Shannon-Wiener index for a given data set can be c

The middle course - classification of the river, The middle course - Classi...

The middle course - Classification of the river The middle course of the river occurs over the foothill belt where the velocity or water is comparatively less which moves a li

What are some examples of biological activities, What are some examples of ...

What are some examples of biological activities in which osmosis plays an important role? Hemolysis (destruction of red blood cells) by entrance of water, the hydric regulation

Cause the inhibition of apoptosis, We now understand that mutations that ca...

We now understand that mutations that cause the inhibition of apoptosis are found in tumors. Because proliferation itself is not induced by the inhibition of apoptosis, explain how

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd