Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Illustrate internal epithelium of the bowel, Q. Is the internal epithelium ...

Q. Is the internal epithelium of the bowel the alike as it was one month ago? The internal epithelial covering of the intestine acts as means of nutrient absorption and as prot

What benefits can commensalism offer to a species, What benefits can commen...

What benefits can commensalism offer to a species? Commensalism may include obtainment of food (for instance, the innocuous bacteria of the human gut), shelter or support (epip

Population growth, Increase in population size is known as population growt...

Increase in population size is known as population growth. It depends upon number of persons added to the population and number of persons lost from the population. Addition in pop

Evolution, how to calculate the frequency of mating with the HW equation?

how to calculate the frequency of mating with the HW equation?

Where in leaves the photosynthetic tissue often located, Where in the leave...

Where in the leaves is the photosynthetic tissue often located? The major photosynthetic tissue is the photosynthetic parenchyma (also called as chlorenchyma, do not confuse wi

Explain about the cellular senescence - geriatric nutrition, Explain about ...

Explain about the Cellular senescence - Geriatric Nutrition? Aging brings about an irreversible state of growth arrest at the cellular level. Though the cells are viable and me

What is gluconeogenesis, What is gluconeogenesis There is accumulation ...

What is gluconeogenesis There is accumulation of lactate, which is released into the blood and taken up by  the liver where  it  is converted to glucose by  the  process called

Types of hypertension, Types of hypertension are as follows: Essential ...

Types of hypertension are as follows: Essential Hypertension is called essential when no apparent cause is suspected or detected. This accounts for almost 90 per cent of pati

Formation of pronuclei , Formation of pronuclei : Information of pron...

Formation of pronuclei : Information of pronuclei, micronucles which is one of the conjugants, plays an important role. Micronucleus undergoes meiosis and forms four haplo

What are the main human diseases caused by prions, What are the main human ...

What are the main human diseases caused by prions? The main called human diseases of such type are the Creutzfeldt-Jacob disease (CJD), the kuru and the Gerstmann-Sträussle-Sch

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd