Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

Viral diseases of domestic animals, Cattle and buffalo diseases Rinde...

Cattle and buffalo diseases Rinderpest: Rinderpest is the most destructive of the virus diseases of cloven-footed animals, viz. cattle, buffaloes, sheep, goats, pigs and wil

Briefly explain about pickled vegetables, Briefly explain about Pickled veg...

Briefly explain about Pickled vegetables These are produced by spontaneous lactic acid fermentation. Fermentation improves the digestibility and wholesomeness  of the product.

Cardiac resynchronization therapy, This innovative, pacemaker-based approac...

This innovative, pacemaker-based approach to the treatment of patients with heart failure who have a wide QRS complex (>140 ms) on 12-lead ECG aims at providing electromechanical c

Digestive system - stomach, STOMACH It is wide, J-shaped, placed obl...

STOMACH It is wide, J-shaped, placed obliquely on left side in upper abdominal cavity so left kidney is slightly down ward. It is 30 cm, long and 15 cm. wide. It has g

What resources are competed for by animals, What resources are competed for...

What resources are competed for by (a) animals, (b) plants?  (a) Animals compete for food, mates and shelter.  (b) Plants compete for light, water and minerals.

State the exocytosis in a skeletal muscle, Exocytosis in a skeletal muscle?...

Exocytosis in a skeletal muscle?   A.  During exocytosis in a skeletal muscle, there will be release of calcium ions from intracellular vesicles in the sarcoplasmic reticulum i

Why is ph regulation important for living beings, Why is pH regulation impo...

Why is pH regulation important for living beings? How do mineral salts participate in this regulation? The potential of hydrogen (pH) is a measure of the amount of hydrogen ion

Class of subphylum uniramia - insecta, Class of Subphylum Uniramia - Insect...

Class of Subphylum Uniramia - Insecta Class Insecta consists of nearly a million described species. There are more species of insects as compared to all the other species of a

Determine energy requirements for athletes, Determine Energy Requirements f...

Determine Energy Requirements for Athletes? You are familiar with the fact that body needs' energy to maintain body temperature, to meet metabolic demands, maintain electrolyte

Explain food intolerances, Food Intolerances Food Intolerances:  The in...

Food Intolerances Food Intolerances:  The intolerances of  the patient for specific food  items must be assessed. For example,  milk may lead  to diarrhoea  in some people whil

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd