Explain transfer from solid culture to liquid media, Biology

Assignment Help:

Explain Transfer from Solid Culture to Liquid Media

The steps involved in this technique are included herewith:

1. Sterilized inoculating loop or needle is touched carefully to the microbial growth in agar plates or in agar slant.

2. After removing the cap/plug from the broth tube and sterilizing its neck, the loop or needle is shaken slightly in sterile broth medium to dislodge the organisms.

After inoculation of the medium (liquid/solid), plates and tubes are kept in the incubator at 37°C or at specific temperature required by microorganism for its growth.  For liquid culture, "shake water baths" can be used at appropriate constant temperature.


Related Discussions:- Explain transfer from solid culture to liquid media

How does the swim bladder of fishes work, How does the swim bladder of fish...

How does the swim bladder of fishes work allowing fishes to control their depth under water? From Hydrostatics it is called that an object does not sink if its density is equal

Wet and dry thermal treatment, Wet and dry thermal treatment Wet therma...

Wet and dry thermal treatment Wet thermal treatment: it is based on exposure of shredded infectious waste to high temperature, high pressure steam. It is inappropriate for the

Reptiles, biting mechanism in snakes?

biting mechanism in snakes?

Explain the functionality of carboxymethyl cellulose, Functionality of CMC ...

Functionality of CMC CMC is soluble in cold water and mainly used for controlling viscosity without gelling. As its viscosity drops during heating, it may be used to improve t

Cells, List structures found in unicellular organisms that are an adaptatio...

List structures found in unicellular organisms that are an adaptation to their free-living life and are not usually found in the cells of multicellular organisms.

Explain about the freeze drying Method, Explain about the freeze drying Met...

Explain about the freeze drying Method? The Techniques of freeze-drying have been developed over the past half-century for the purpose of preserving certain biological material

What is meiosis, What is Meiosis ? Meiosis starts with a diploid cell a...

What is Meiosis ? Meiosis starts with a diploid cell and forms four haploid, or 1n, (or "n") germ cells. These n germ cells fuse during fertilization with another germ cell to

Describe pericardial rub and their characteristic, Describe Pericardial Rub...

Describe Pericardial Rub and their Characteristic ? Generation of sound: The sound is generated due to rubbing of visceral and parietal pericardial surfaces against each other

Brain, why left brain controls right side of the body and right brain contr...

why left brain controls right side of the body and right brain controls left side of the body....?????

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd