Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

Scintigraphy or nuclear medicine, S c inti g r ap h y ...

S c inti g r ap h y or nuclear medicine: Scintigraphy was introduced in veterinary medicine in 1970s. It is highly sensitive and can detect bone dama

Define water distribution and compartments of body water, Define Water Dist...

Define Water Distribution and Compartments of Body Water? Each one of us has a veritable 'sea within', held in place by multiple membranes and our protective envelope of skin.

Urea molasses mineral block (ummb) licks, Urea molasses mineral block (UMMB...

Urea molasses mineral block (UMMB) licks Development of urea molasses mineral block licks is another technology being increasingly used in several parts of India and such lick

Determine st-segment elevation, Q. Determine ST-Segment Elevation? Ans...

Q. Determine ST-Segment Elevation? Ans. Exercise induced ST-segment elevation is common in subjects with post MI stress tests in leads where Q-waves are present. It has be

Explain the vertical implant position, Vertical Implant Position: The i...

Vertical Implant Position: The implant can be submerged in the bone up till the level, where it is surface treated or further embedded till its shoulder, depending upon the sit

What are the destinations of those oxygen atoms, Q. Photosynthesis is the m...

Q. Photosynthesis is the most significant producer of molecular oxygen (O2) on our planet. From which molecule do oxygen atoms liberated by photosynthesis come and from which other

Define recommended dietary allowances for vitamin d, Define Recommended Die...

Define Recommended Dietary Allowances for Vitamin D? The recommendations of vitamin D are expressed as IU. 1 IU is defined as the activity contained in 0.025 mcg of cholecalci

How do bones grow, Q. How do bones grow? Bones form and develop through...

Q. How do bones grow? Bones form and develop through a process known as ossification. There are two types of ossification: endochondral ossification and intramembranous ossific

Excretory system, is thare any excretory system found in echinoderms

is thare any excretory system found in echinoderms

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd