Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

Explain intraoral barrier techniques, Q. Explain Intraoral Barrier Techniqu...

Q. Explain Intraoral Barrier Techniques? Rubber dam A rubber dam is used whenever possible for improved vision and access and to reduce dental personnels' exposure to micr

What is the auditory tube and its function, Q. What is the auditory tube an...

Q. What is the auditory tube and its function? Auditory tube (Eustachian tube) connects each middle ear to the throat. This tube conducts air between tympanic cavity and outsid

Steps for elongation , a) The elongation level of translation in eukaryo...

a) The elongation level of translation in eukaryotes requires three elongation factors, eEF-IB, eEF-1A and eEF-2, that have similar functions to their prokaryotic counterparts EF-G

Stratification of vegetation - qualitative characters, Stratification of Ve...

Stratification of Vegetation - Qualitative Characters Stratification of vegetation is another important feature of plant communities. There are different vertical strata in di

Describe the principles of dna sequencing, The ability to sequence DNA has ...

The ability to sequence DNA has led to a revolution in molecular biology. a) Describe the principles of DNA sequencing. b) A single sequencing reaction yields at best 1500 ba

What is the mechanism of elisa, ELIZA stands for enzyme linked immune diffu...

ELIZA stands for enzyme linked immune diffusion assays. It works on standard of antigen antibody reaction.

Trout beck zone - lotic ecosystem, Trout beck zone - Lotic Ecosystem T...

Trout beck zone - Lotic Ecosystem This is larger and more constant than the head stream. The greater volume of torrential water carves channels into exposed rock floor (bed ro

Forms of soil water, Forms of Soil Water Gravitational Water or Groun...

Forms of Soil Water Gravitational Water or Ground Water: After a heavy rain or irrigation, much of the water drains or sinks downwards. This is called gravitational water.

Explai total fertility rate and replacement level, Differentiate between To...

Differentiate between Total Fertility Rate (TFR) and Replacement Level (RL) Due to uncontrolled excessive hunting the population of tigers in a forest becomes Zero. Discuss the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd