Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

Explain the sarcomere, For a sarcomere of a skeletal muscle, define the fol...

For a sarcomere of a skeletal muscle, define the following terms: A is the length of the A Band; H is the length of the H Zone; I is the total length of the I Bands in the sarcomer

Biogeochemical cycle, what are the effects of mining and farming on the pho...

what are the effects of mining and farming on the phosphorus cycle?

Excretory system - kidney, Kidney - 2 in number. Dark red. Sl...

Kidney - 2 in number. Dark red. Slightly flattened. Bean shaped. 10 cm. long, 5 cm wide and 4 cm thick (In rabbit 20-25 mm). Weighing 150 gm in male 135 gm in fema

Define beriefly about the phytoestrogens, Define beriefly about the Phytoes...

Define beriefly about the Phytoestrogens? You may be aware of the surging interest in phytoestrogens (PE) especially in connection with osteoporosis. This section briefly discu

Types of changes encountered during the support phase, What are the types o...

What are the types of changes encountered during the Support phase Adaptation - To accommodate changes to its environment Correction - To uncover defects in the Software

Define the structure of vitamins, Define the Structure of Vitamins? Fo...

Define the Structure of Vitamins? For your convenience, however, we have given the structures of these vitamins here in the text. Look at Figure which illustrates the fat-solu

Viral vectors, Viral vectors   There are a large number of viral vector...

Viral vectors   There are a large number of viral vectors available which can transfer the desired sequence properly into the host. Examples of the viral vectors are as follows

Write the meaning of gestational diabetes mellitus, Q. Write the meaning of...

Q. Write the meaning of Gestational Diabetes Mellitus? Gestational Diabetes Mellitus (GDM) is defined as glucose intolerance of any severity that has its onset or is first reco

Atp molecules produced for each glucose molecule, Q. How many ATP molecules...

Q. How many ATP molecules are produced for every glucose molecule used in fermentation? And How many ATP molecules are produced for each glucose molecule used in aerobic respiratio

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd