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Explain the Voges Proskauer (VP) Test?
As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.
Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.
Explain Glycerol esters of fatty acids Glycerol esters of fatty acids, which make up to 99% of the lipids of plant and animal origin have been traditionally called fats and oi
Define Surface Sampling Food contact surfaces (e.g. storage tank, packaging material, ripening room, utensils, equipments, refrigerators etc.) which directly or indirectly (wal
Q. Areas affected in peptic ulcer? The areas affected in peptic ulcer (due to erosion) can be: 1. Lower part of the oesophagus. 2, stomach (lesser curvature - an urn, whe
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Explain the following and give their values in a normal human adult: a) Tidal volume b) Expiratory reserve volume c) Inspiratory capacity.
The effect of natural selection on colonies or population favouring one group in preference to other may be referred to as group selection. A group can be the smallest collection o
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Give one example in each case of (a) a fixed joint, (b) a ball and socket joint, (c) a hinge joint. (a) The bones of the skull, the junction of pelvic gi
Q. ST Depression only on Recovery? Although ST depression during exercise often persists into recovery, it may not manifest until exercise has been terminated. The reasons for
Define Procedure for estimation of vitamin c - colorimetric method? Carry out the experiment following the instructions given herewith: 1. Prepare a series of tubes for blan
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