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Explain the Voges Proskauer (VP) Test?
As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.
Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.
Q. What are the pentoses? What are the vital roles of pentoses in DNA and RNA molecules? Pentoses are carbohydrates whole of five carbons. The DNA molecule is made of a sequ
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a) Explain somatic hybridisation? b) Describe the various steps involved in the process. Mention any two uses of somatic hybridisation
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how nematodes adapted to their mode of feedings
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Any significant variation in the internal temperature could have damaging effects on the body's enzymes. Homeotherms or warm-blooded animals including human can however generate co
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