Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

Which part of the teeth is it important to remove plaque, From which part o...

From which part of the teeth is it particularly important to remove plaque. It is most significant to remove plaque from among the teeth and from the region where the gum cover

How to planning the daily diet for underweight patients, How to Planning th...

How to Planning the Daily Diet for Underweight Patients? As mentioned above you need to add calories gradually to the diet. A practical way of doing so is to take the present i

How many people carry the allele, Phenylketonuria is a severe form of menta...

Phenylketonuria is a severe form of mental disability caused by a homozygous recessive allele. The condition affects about 1 in 10,000 neborn Caucasians. Estimate the frequency of

Conformational protection - azotobacter, Conformational Protection - Azotob...

Conformational Protection - Azotobacter In conformational protection a Fe-S redox protein provides protection to the enzyme. The protein gets oxidised in the presence of oxyge

Human body, what are the importance in studying animal tissues?

what are the importance in studying animal tissues?

Percussion - criteria for endosteal implants, Percussion - criteria for end...

Percussion - criteria for endosteal implants? It is neither an indicator of clinical health nor of rigid fixation. Ringing sound that occurs on percussion corresponds to the pr

What are the main parts of ferns, What are the main parts of ferns? Fer...

What are the main parts of ferns? Ferns are constituted by small roots that come downwards from the rhizome (stem, often orizontalized). The fronds also arise from the rhizome.

How a glycosidic bond would be present, A glycosidic bond would be present ...

A glycosidic bond would be present in: Select one: a. acetone. b. methyl-alpha-D-glucose. c. 2-deoxy-beta-D-ribose. d. glucose-6-phosphate. e. fructose-1, 6-bisph

What are the symptoms of brown-sequard syndrome, What are the Symptoms of B...

What are the Symptoms of Brown-Sequard syndrome Symptoms of Brown-Sequard syndrome usually appear after an affected individual experiences a trauma to the neck or back. First s

What is the water cycle, Q. What is the water cycle? The water cycle re...

Q. What is the water cycle? The water cycle represents the recycling and circulation of water in nature. The Liquid water on the planet surface is heated by the sun and turns i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd