Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

Lungs, what''s the role of lungs in terms of excretion? Like the trachea wh...

what''s the role of lungs in terms of excretion? Like the trachea what''s its role in excretory system?

What is alkaline phosphatase, Question 1 Discuss the quantitative methods ...

Question 1 Discuss the quantitative methods for the estimation of urinary proteins. What are the instructions to be given to a patient while collecting urine for biochemical exami

Explain glucokinase, Glucokinase In the fed state, clearance of blood  ...

Glucokinase In the fed state, clearance of blood  glucose is mainly by  liver via glucokinase. Glucokinase, which  is an  inducible enzyme, removes most of the blood glucose fr

What is intra oral and extra oral, What is Intra oral and Extra Oral A...

What is Intra oral and Extra Oral A) Intra oral a) Frontal in occlusion b) Left and right occlusion (Using intra oral photography mirror) c) Occlusal view maxillary

To study if bacteria grow better where it is dark or light, To study if bac...

To study if bacteria grow better where it is dark or light Inoculate two sterile dishes as before. Label one 'dark' and the other 'light'. Place the 1st dish in a darkwarm plac

Commensalism, #quesWhat benefits can commensalism offer to a species..

#quesWhat benefits can commensalism offer to a species..

Recent concerns of food safety, Normal 0 false false fal...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Differences between hypothesis and theory, DIFFERENCE S BETWEEN HYPOTHESIS...

DIFFERENCE S BETWEEN HYPOTHESIS AND THEORY -     1. Hypothesis   It is a reasoned or educated guess about a     1.

Major information storage molecule, Following statement true for prokaryoti...

Following statement true for prokaryotic or eukaryotic? They use deoxyribonucleic acid as their major information storage molecule.

Effect of alcoholic beverage, Q. Why does the urinary volume increase when ...

Q. Why does the urinary volume increase when alcoholic beverages are ingested? Alcohol inhibits the ADH (antidiuretic hormone) secretion by the hypophysis and Low ADH reduces t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd