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Explain the Voges Proskauer (VP) Test?
As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.
Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.
Define Reagent for Separation of Amino Acid by Paper Chromatography? The following reagents will be required for carrying out the experiment: 1. Amino acid solution- Weig
Autotrophic and Heterotrophic Succession The succession where initially the green plants are much greater in quantity than the animals, is known as autotrophic succession. Su
Define pisces
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Q. Define Primary and Somatic Sensory Area? Primary Sensory Area - Located in postcentral gyrus in parietal lobes (just posterior to central sulcus). Neurons here receive info
Explain Precautions for preparation of general purpose media 1. Dissolve ingredients one by one in distilled water. 2. Check and set the medium to appropriate pH. 3. Auto
Pathophysiology Insufficient amount of iron present in the body leads to reduction in serum transferrin (Serum betaglobulin, which binds and transports iron) saturation. T
Regeneration Leading To Asexual Reproduction Regeneration is described as the replacement of the lost parts of the body of an organism. This capacity is present in approximat
What are the fruiting bodies present in some fungi? Fruiting bodies are structures made of hyphae that project radially from the superior portion of the peduncle of some fungi.
Explain Biochemical Tests Bacterial Testing? It deals with the various biochemical tests used to differentiate microorganisms. Identification of an unknown organism isolated fr
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