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Explain the Voges Proskauer (VP) Test?
As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.
Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.
Define the Functional Properties of Proteins? a) Hydration properties (dependent on protein-water interactions), which include properties like swelling, adhesion, dispersibilit
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Spina Bifida Occulta There is no protrusion of the meninges but the vertebrae are bifid. There are three components of the spina bifida occulta. a) Vertebral- there is
Each of the properties that follow is characteristic of the carbon atom. In each case, indicate how the property contributes to the role of the carbon atom as the most important at
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