Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

Heparin, HE P ARIN It is highly sulphated mucopolysaccharide made ...

HE P ARIN It is highly sulphated mucopolysaccharide made of alternate units of glucosamine and glucuronic acid. Heparin is mainly produced by liver and mast cells. I

Define vitamins b12 deficiency in vegans, Define Vitamins B 12 Deficiency ...

Define Vitamins B 12 Deficiency in Vegans? Because plants do not synthesize vitamin B 12 , individuals who consume diets completely free of animal products (vegan diets) are a

Explain insect resistance, In 2003 nearly 70 million hectares globally were...

In 2003 nearly 70 million hectares globally were planted in genetically modi?ed crops. Of these, 73%  were plants such as soybean, corn and cotton, which were modi?ed to be he

Define the recommended dietary allowances of vitamin k, Define the Recommen...

Define the Recommended Dietary Allowances of Vitamin K? Recommended dietary intakes have not been suggested for different age groups or gentler. The safe levels of intake have

How boiling used for sterilization, Q. How Boiling used for sterilization? ...

Q. How Boiling used for sterilization? Though highly unreliable as a sterilization technique and more effective for disinfection, it is used very often. Boiling water in hot wa

Define quantitative procedures in carbohydrates, Define Quantitative Proced...

Define Quantitative Procedures in Carbohydrates? You have learnt how to identify some individual monosaccharides, disaccharides and polysaccharides. However, if we were asked t

Cellular and molecular events of bone formation, Q. Summarize the cellular ...

Q. Summarize the cellular and molecular events of bone formation? The events involved in osseous wound healing at implants recapitulate the events of wound healing. Haemostasi

Define transverse section of body of male ascaris, Define Transverse sectio...

Define Transverse section of body of male Ascaris? 1. Body wall consists of the following: (a) Cuticle is the outermost layer. It is a thick, tough, elastic membrane

Explain the process of absorption of fats, Explain the process of Absorptio...

Explain the process of Absorption of Fats? After digestion, only 25% of triglycerides are broken completely to glycerol and fatty acids. Major digestion product is 2-monoacylgl

How are artificial and natural pearls created, Q. How are artificial and na...

Q. How are artificial and natural pearls created? Pearls are made from small strange particles that deposit between the mantle and the shell of the oyster. These particles trig

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd