Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

Values of dpd for plant cells under hypertonic media, Q. What are the value...

Q. What are the values of DPD for plant cells under hypertonic, hypotonic and isotonic media? In plant cells under hypertonic medium there is loss of water for the external, SF

she kept forgetting her appointment with the dentist, Listed below are exa...

Listed below are examples of behaviors that typify a defense mechanism at work. For each example choose the correct defense mechanism and explain the reason for your choice. 1. J

Reproduction, The fusion of two individuals with each acting as a gamete is...

The fusion of two individuals with each acting as a gamete is known as

Biological stress, Biological Stress Since in nature, the various orga...

Biological Stress Since in nature, the various organisms do not live in complete isolation from others, stress to a plant species might also be caused by what other organisms

Global Warming Prevention, Preventive steps for global warming :  Growing ...

Preventive steps for global warming :  Growing trees in vacant places. Growing social forests and protect them. Promoting abundant growth of phytoplankton plant species in marine

Define titrimetric estimation of carbohydrates, Define Titrimetric Estimati...

Define Titrimetric Estimation of Carbohydrates? The Fehling's Soxhlet method is the commonly used method for titrimetric estimation of carbohydrates. In the following section y

Aril - seed appendages, Aril - Seed Appendages It is an outgrowth that...

Aril - Seed Appendages It is an outgrowth that arises from the funicle or the testa near the raphe and covers the seed partially or completely. It is often referred to as the

Tissue synthesis for wound healing in nutritional care, Explain the Tissue ...

Explain the Tissue Synthesis for Wound Healing in Nutritional Care? Tissue proteins are synthesized only by amino acids brought to the tissues by circulating blood. The necessa

Define functions of sodium and chloride, Define Functions of sodium and chl...

Define Functions of sodium and chloride? So far you have learnt that most minerals participate in important functions of body as they support the activity of specific enzymes.

Explain the term microfilaments, Explain the term Microfilaments ? Mi...

Explain the term Microfilaments ? Microfilaments are fine fibrils, only 7 nm in diameter, composed of a protein called actin. Actin is one of the two major proteins involved

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd