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Explain the Voges Proskauer (VP) Test?
As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.
Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.
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Explain the Direct Surface Agar Plating Method? In the direct surface agar plating method, molten agar is placed on to the surface to be assessed. Upon hardening, it is incubat
ISOMERISM AND STERIOISOMERISM IN HEXOSE SUGERS
a nutrient agar plate labelled 10^(-5)ml had 154 colonies after incubated. what is the cell density in the original sample
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Echocardiographic evaluation should be performed in all patients with clinically suspected IE, including those with negative blood cultures. Not only is TEE the preferred approach
difference between axon and cyton
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