Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

Define the general mortality and morbidity risk, Define the General Mortali...

Define the General Mortality and Morbidity Risk? Obesity increases the risk of morbidity and mortality. The obese are more prone to developing morbidities or other chronic dise

Define male and female ascaris lumbricoides, Define Male and Female Ascaris...

Define Male and Female Ascaris Lumbricoides? Ascaris lumbricoides unlike most free-living nematodes and a large number of parasites of plants and animals are not tiny or micro

Explain about low blood sugar, Q. Explain about Low blood sugar? Low bl...

Q. Explain about Low blood sugar? Low blood sugar can also be caused by accidentally taking too much of insulin. When this occurs, more insulin is present in the body than nee

Define muscular endurance component for physical fitness, Define Muscular e...

Define Muscular endurance component for physical fitness? It is the ability of a muscle, or a group of muscles to sustain repeated contractions or to continue applying force ag

Phylum arthropoda, PHYLUM ARTHROPODA Definition and Introduction ...

PHYLUM ARTHROPODA Definition and Introduction Bilateral  and protostomial eucoelomate  eumetazoa with metamerically  segmented  and each  segment bearing a pair  of  join

Interaction of insect hormones, Interaction of insect hormones in the proce...

Interaction of insect hormones in the process of metamorphosis The organs and the hormones usually included in metamorphosis of insects. This is since despite the fact that t

Determine the syndrome of listeriosis, Determine the Syndrome of Listerios...

Determine the Syndrome of Listeriosis Syndrome: Listeriosis in humans is not characterized by a unique set of symptoms since the course of the disease depends upon the state

Etiologic factor of congestive cardiac failure, Q. Etiologic factor of Cong...

Q. Etiologic factor of Congestive Cardiac Failure? Congestive heart failure develops over a period of time when the necrotic tissues are not replaced by functional connective c

How can nutrients are classified, According to their functions how can nutr...

According to their functions how can nutrients are classified? One possible and utile functional classification for nutrients is the one that divides them into energetic, struc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd