Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

Ciliates - parasitic protozoan, Ciliates - Parasitic Protozoan The onl...

Ciliates - Parasitic Protozoan The only ciliate of medical and veterinary importance is Balantidium coli which inhabit the large intestine of man, apes, monkeys and pigs. B. c

Determine the molecular function, Please answer the following three questio...

Please answer the following three questions on Sequence Z: Metadata The GO Ontology is a very widely-used resource in the bioinformatics community as a tool to annotate

Vitamin C, What is pasteurization of fruit juice?

What is pasteurization of fruit juice?

Are birds rare in polar regions, How different are reptiles and birds conce...

How different are reptiles and birds concerning the maintenance of body temperature? Are birds rare in polar regions? Reptiles are heterothermic, i.e., they do not control thei

How can we determine solutions, How can we determine Solutions Solution...

How can we determine Solutions Solutions are a homogeneous mixture of two or more different substances. This means that the molecules of the dissolved substances (solute) and t

Differences between hypothesis and theory, DIFFERENCE S BETWEEN HYPOTHESIS...

DIFFERENCE S BETWEEN HYPOTHESIS AND THEORY -     1. Hypothesis   It is a reasoned or educated guess about a     1.

Protozoa, advantages and disadvantages of protozoa

advantages and disadvantages of protozoa

Genetic reservoir - conservation of wildlife, Genetic Reservoir - Conservat...

Genetic Reservoir - Conservation of Wildlife  Despite the present and future economic and health importance to human beings, very little is known about most of the earth's 1.

Minerals requirements for dyslipidemia, Q. Minerals requirements for dyslip...

Q. Minerals requirements for dyslipidemia? Minerals: The three most important minerals are chromium, zinc and magnesium. These minerals play a critical role in maintaining p

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd