Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

Triploblastic, What Is the advantage of having a coelomic body cavity

What Is the advantage of having a coelomic body cavity

Goat pox, Goat pox Goat-pox is common in occurrence, and its nature is ...

Goat pox Goat-pox is common in occurrence, and its nature is similar to sheep pox. The incubation period varies from 5 to 10 days. The disease tends to attack male kids and ewe

Right coronary artery, Right coronary artery arises from the anterior aorti...

Right coronary artery arises from the anterior aortic sinus. It runs down along the anterior part of the coronary sulcus, reaches the lower border of heart turns round the lower bo

What is a terrestrial organism, Explain what is a terrestrial organism? ...

Explain what is a terrestrial organism? Ans) 'Terra' is the Latin word for earth. Thus, an animal that lives on the surface of the earth is known as terrestrial. This is the si

Explain cloning vector, Cloning vector: The DNA molecule originating from ...

Cloning vector: The DNA molecule originating from the virus, a plasmid, or the cell of a higher organism into which an another DNA fragment of appropriate size can be integrated w

Show some fungal diseases transmitted by animal feces, Q. What are some fun...

Q. What are some fungal diseases transmitted by animal feces? Pigeon and Bat feces can carry Histoplasma capsulatum, the fungus agent of histoplasmosis. The infection is transm

Urine sugar testing, Testing for urine sugars is not recommended for either...

Testing for urine sugars is not recommended for either diagnosis or monitoring of patients with diabetes. This is because a urine sugar is not a reliable test. When no facilities a

Cross-pollination - types of pollination, Cross-Pollination - Types of Poll...

Cross-Pollination - Types of Pollination In this kind of pollination the pollen from anther of one individual is transferred to the stigma of another individual of the same sp

Explain simple sugar, Simple sugars are monosaccharides consisting of a si...

Simple sugars are monosaccharides consisting of a single polyhydroxy aldehyde or ketone unit. Their general formula is (CH 2 0) n (n=3 to 7).

What is passive transport, What is Passive Transport? Passive Transpor...

What is Passive Transport? Passive Transport :  Specialized proteins called permeases also move specific substances across the cell membrane in a process called carrier transp

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd