Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

Define mechanisms for antinutritional effect on human, Define mechanisms fo...

Define mechanisms for antinutritional effect on Human? There are several possibilities: reduced availability of amino acids to cancer cells by reversing the carcinogen-induced

Cartoon, A dialogue and cartoon between haemoglobin and chlorophyll

A dialogue and cartoon between haemoglobin and chlorophyll

Medicinal value - conservation of wildlife, Medicinal Value - Conservation ...

Medicinal Value - Conservation of Wildlife About 40% of all drugs used throughout the world have active ingredients extracted from plants and animals. The world-wide annual sa

What do you understand by herbarium ethics, Q. What do you understand by He...

Q. What do you understand by Herbarium ethics? You should also know that herbaria provide us with policy statements including such information as 1) filing arrangements, 2) res

Why dairy or milk product are important for human body, Why Dairy or milk p...

Why Dairy or milk product are important for human body? This first food of mammals is rich in body-building proteins and bone-forming calcium, besides being the only source of

Signify platelets of blood, Which of the subsequent statements concerning p...

Which of the subsequent statements concerning platelets is INCORRECT. Platelets: a) Are between 1/2 and 1/3 the diameter of the red cell b) Are roughly disk-shaped c) Hav

Explain the cell cycle in details, Explain the cell cycle in details? M...

Explain the cell cycle in details? Most cells of higher organisms follow a cyclic pattern of division. The bulk of the cycle consists of the stage known as interphase, the peri

Define the needs for enteral feeding, Define the needs for Enteral Feeding?...

Define the needs for Enteral Feeding? For enteral feeding following need to be considered: - In preterm calorie density to be 0.8 Kcal/ml, - LBW formulas used: protein -

Describe wide split s in details, Describe Wide Split S in details? It ...

Describe Wide Split S in details? It is a persistent split that widens on inspiration to at least 60 msec. May be due to delay in P, e.g. RBBB or due to early A, as occasionall

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd