Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

Probability concept of requirements vs risk of deficient, Define Probabilit...

Define Probability concept of requirements vs risk of deficient and excess intake? Figure best illustrates the concept of a requirement for an essential nutrient. The relations

Explain adverse effects of foscarnet, Explain Adverse Effects of foscarnet ...

Explain Adverse Effects of foscarnet  Renal dysfunction often develops during treatment with foscarnet and is usually reversible, but renal failure requiring dialy sis may occu

Define conical flask - nutritional biochemistry, Define Conical Flask - Nut...

Define Conical Flask - Nutritional Biochemistry Conical flask is a piece of chemistry laboratory equipment, a container often made of glass, which has a narrow cylindrical mout

Membrane oxygenators-type of oxygenators , Membrane Oxygenators: They are...

Membrane Oxygenators: They are more physiological and are similar to natural lungs. There is separation of blood and gas by membrane across which gas exchange takes place. There

Extractive use of biodiversity, Q. Extractive use of biodiversity? This...

Q. Extractive use of biodiversity? This includes direct use such as harvesting of wild plant species for use as food, fuel, fodder, fibre, shelter or medicine and hunting anima

Major groups into which the study of the plants is divided, Q. What are the...

Q. What are the four major groups into which the study of the plants is divided? In Botany the plant kingdom is divided into pteridophytes, bryophytes, angiosperms and gymnospe

Left main coronary artery disease, Left Main Coronary Artery Disease (LMCA...

Left Main Coronary Artery Disease (LMCAD) : Stenosis of 50 per cent or more of the left main coronary artery is an indication. If or surgery. Results of medical management are ver

How age affects the bmr, How Age affects the BMR? The BMR per unit weig...

How Age affects the BMR? The BMR per unit weight also varies with age, being higher in children and lower in the elderly. The loss of FFM with ageing is associated with a decli

Show upsloping st-segment, Q. Show Upsloping ST-Segment? There are few ...

Q. Show Upsloping ST-Segment? There are few subjects in exercise literature that has generated more controversy than the significance of up sloping ST-segments. The up sloping

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd