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Explain the Voges Proskauer (VP) Test?
As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.
Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.
Explain the Gram Staining technique? Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staini
What is the classification of protozoa with examples
Body Fluids of Marine Teleost The body fluids of marine teleost are hypotonic to seawater, so these fishes lose water to the environment especially across the gill epithelium.
Q. Explain Aortic Valvuloplasty? Valvar aortic stenosis accounts for 4-6 per cent of CHD. LV Outflow Tract obstruction eventually leads to LV dysfunction and congestive heart f
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Define the Toxicity of Vitamin B 12 ? Intake of 1000 μg vitamin B 12 has never been reported to have any side-effects. Similar large amounts have been used in some preparati
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