Explain the voges proskauer (vp) test, Biology

Assignment Help:

Explain the Voges Proskauer (VP) Test?

As it has been discussed in previous exercise, second group of enteric bacteria produces acid during early incubation which rapidly converted to non-acidic components, i.e., 2, 3 butanediol and acetoin (acetylmethylcarbinol). These components can be detected by using Barritt's reagent, which is a mixture of alcoholic alpha-naphthol and 40% potassium hydroxide solution.

Acetylmethylcarbinol is oxidized to a diacetyl compound in presence of the alpha-naphthol catalyst and a guanidine group present in the peptone of MR-VP medium. This results in formation of a pink complex imparting rose red colouration to a medium after 15 minutes. Development of rose red colour indicates positive VP reaction while absence of it is a negative result.


Related Discussions:- Explain the voges proskauer (vp) test

What is the major constituent of the cell wall of bacteria, Q. According to...

Q. According to their morphology how bacteria are classified? Bacteria present different morphological patterns, a bacterium can be classified into bacillus, coccus, spirochete

How is the genetic determination of sex established in human, How is the ge...

How is the genetic determination of sex established in humans? In the diploid genome of human beings there are 46 chromosomes, 44 of them are autosomes and two are sex chromoso

Biochemistry starch and dextrin test, Why there is difference in the colour...

Why there is difference in the colour obtained for the dextrin solution (ammonium sulphate and dextrin )when brought to iodine test Respetively by first half saturation test and t

Is fecundation in amphibians internal or external, Q. Is fecundation in amp...

Q. Is fecundation in amphibians internal or external? In this aspect are amphibians evolutionarily proximal to fishes or to reptiles? In the majority of the amphibian species f

Heart of cockroach, Heart of cockroach: In cockroach, heart is situa...

Heart of cockroach: In cockroach, heart is situated in pericardial sinus. It has13 chambers These are one behind the other. Each chamber opens into preceeding one. The

State about intra oral periapical radiographs, Intra Oral Periapical Radiog...

Intra Oral Periapical Radiographs These can either be taken conventionally or by the help of RVG (RADIO VISUO GRAPHY).  RVG offers certain benefits over conventional X-Rays suc

Fixation and movement to echinoderms, Q What is the system that permits fix...

Q What is the system that permits fixation and movement to echinoderms? The system that permits fixation and movement to substrates in echinoderms is called the ambulacral syst

State in brief about respiration, State in brief about respiration When...

State in brief about respiration When we breathe in the air, our chest expands and air is taken in the lungs and when we breathe out chest returns to its resting position. This

Gastrulation, Gastrulation  Gastrulation is the process through whic...

Gastrulation  Gastrulation is the process through which the presumptive areas of organ specific rudimetns present on the surface of blastula move to their specific position

Behaviour change communication, Behavior means responses or reactions or ac...

Behavior means responses or reactions or acting in any situation. Change means act of changing or to become different or to modify. Behaviour Change can refer to any modifica

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd