Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

.excretion, name the blood vessel which bring waste product to kidney

name the blood vessel which bring waste product to kidney

Define dietary management of cancer patients, Define Dietary Management of ...

Define Dietary Management of Cancer Patients and Feeding Problems Related To Cancer Therapy? We took an overview about the general nutritional requirements of cancer patients.

Bee and silkworm diseases, BEE DISEASES - Suffer from nosema diseases...

BEE DISEASES - Suffer from nosema diseases caused by Nosema apis. SILK WORM DISEASES - Pebrine disease caused by Nosema.

Determine how nutrition used to prevent dehydration, Determine How Nutritio...

Determine How Nutrition used to Prevent Dehydration? Maintaining optimal hydration status along with sufficient muscle glycogen stores delays the onset of fatigue and enables

Explain the sticky films - food microbiology, Explain the Sticky Films - Fo...

Explain the Sticky Films - Food Microbiology? Sticky films or tape are pressed against the surface to be assessed. Exposed film/tape is then pressed on the agar plate and analy

What proportion of offspring will have same rflp pattern, A laboratory mous...

A laboratory mouse homozygous for an RFLP marker is mated to a wild mouse that is heterozygous for that marker. One of the heterozygous individuals resulting from this cross is mat

Illustrate in detail about the cell theory, Illustrate in detail about the ...

Illustrate in detail about the Cell theory All living organisms are composed of cells, and they are the basic structural and functional units of life, just like the atom is a

Microfilaments - role of cytoskeleton structures, Microfilaments - Role of ...

Microfilaments - Role of Cytoskeleton Structures Microfilaments are as well present in all animal cells. They are found singly throughout the cytoplasm organized like a meshwo

How does temperature affect the action of enzymes, How does temperature aff...

How does temperature affect the action of enzymes upon their substrates? There are explained temperature ranges under which enzymes operate and there is a specific temperature

Explain the flower in diversity of life, Explain the Flower in diversity of...

Explain the Flower in diversity of life? In order for plants to evolve from an aquatic existence to a terrestrial existence, specific adaptations in their basic modes of reprod

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd