Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Therapeutics for infective endocarditis, Therapeutic   Accurate identif...

Therapeutic   Accurate identification of the infecting organism is the key to successful treatment.  Antibiotics as per the sensitivity study in high doses IV is given.

Eletrons., Atom has how many eletrons

Atom has how many eletrons

Infective endocarditis, Despite improvements in health care, the incidence ...

Despite improvements in health care, the incidence of infective endocarditis has not decreased over the past decades. Infective endocarditis is lethal if not aggressively treated w

Structure of golgi body, STRUCTURE Golgi body consists of :- (1 ) ...

STRUCTURE Golgi body consists of :- (1 ) Cisternae These are plate like unbranched structure with dilated ends. One golgi body has 4-8 cisternae. Mono cisternal

Formation of double bonds, In eukaryotes  the SER has enzymes  able to begi...

In eukaryotes  the SER has enzymes  able to begin  double  bonds into fatty acyl CoA molecules  in an oxidation  reaction  which uses molecular  oxygen.  This reaction is catalyzed

Mulluscas., economic importance of mulluscas

economic importance of mulluscas

What is isotope switching, It is conversion of antibody class to another re...

It is conversion of antibody class to another resulting from genetic rearrangement of heavy chain same region genes in B cells. Isotope switching is also known as class switching.

What treatment should be used for constrictive pericarditis, Q. What treatm...

Q. What treatment should be used for constrictive pericarditis? Medical Treatment 1) Judicious use of diuretics to alleviate systemic congestion. This may be enough in s

Define key concepts and facts of polysaccharides, Define Key Concepts and F...

Define Key Concepts and Facts of Polysaccharides? 1. All polysaccharides contain several monosaccharide units. 2. All monosaccharide units are joined to each other by glycosidi

Explain the anabolic phase - dietary management for burns, Explain the Anab...

Explain the Anabolic Phase - Dietary Management for Burns? During this period the patient is well hydrated and the reactions due to metabolic stress are under control. The pati

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd