Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Assignment, parts involving in circulatory system

parts involving in circulatory system

What is thyroid disease, Q. What is thyroid disease? Thyroid disease: T...

Q. What is thyroid disease? Thyroid disease: Thyroid disease is associated with angina. Anginas pectoris presents itself in the form of speific symptoms which tend to re- oc

Secondary egg envelopes, Secondary Egg Envelopes These are secreted b...

Secondary Egg Envelopes These are secreted by oviducts and other accessory parts of genital organs while the egg is passing through them from ovary to the exterior. a) In

.rRNA , rRNA structure and synthesis assignment

rRNA structure and synthesis assignment

Theskin, drawandlabelthemajorendocrineglandsofthehumanbody

drawandlabelthemajorendocrineglandsofthehumanbody

Express an example of directional selection, The peppered moth experiment w...

The peppered moth experiment was an example of directional selection; due to environmental conditions the directional selection shifted from one extreme to the other in 1850s Engla

Define about the chemotherapy, Define about the Chemotherapy - Cancer? ...

Define about the Chemotherapy - Cancer? Chemotherapy results in lot of side effects. This is because the drug effects are not specific to cancer cells alone. Even the host cell

Birth control - permanent methods, PERMANEN T METHOD - 1. Vasectomy in...

PERMANEN T METHOD - 1. Vasectomy in male. 2. Tubectomy in female. 3. Leproscopy is used in tubal ligation , to ligate fallopian tubes.

Does an increase of h+ cause the hemaglobin, Does an increase of H+ cause t...

Does an increase of H+ cause the hemaglobin oxygen binding curve to shift to the left or right? Explain.

Explain the treatment of iron deficiency anaemia, Explain the Treatment of ...

Explain the Treatment of iron deficiency anaemia? Oral iron is the preferred method of treatment of IDA. The dosage is decided depending on the severity of the condition. Gener

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd