Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Embryology, what is the full name of Balfour in Balour''s law of cleavage?

what is the full name of Balfour in Balour''s law of cleavage?

fungi, Fungi The study of fungi (s. fungus) is called mycology. Fungi...

Fungi The study of fungi (s. fungus) is called mycology. Fungi are unicellular or multicellular chemoheterotrophic eukaryotes. They may be grouped into yeasts, molds and funga

Define the basic types of drying process, Define the Basic Types of Drying ...

Define the Basic Types of Drying Process? Primarily, there are three basic types of drying process: a) Sun drying and solar drying;  b) Atmospheric drying including batch

Explain degradation of carbohydrates, Q. Explain Degradation of Carbohydrat...

Q. Explain Degradation of Carbohydrates? Ans. The carbohydrates that are naturally present in foods, you may already know, can be divided into monosaccharides, disaccharid

What purpose does sleep serve for the brain, What purpose does sleep serve ...

What purpose does sleep serve for the brain? A. We don't yet know full answer to this fundamental question however neuroscience is providing intriguing clues. It's increasingly

Effects of solid waste pollution, The improper handling and transfer of the...

The improper handling and transfer of the solid wastes results in various health and environmental hazards, such as: 1.      Spoilage of landscape: Municipal wastes heap up o

Determine about the secondary minerals, Determine about the Secondary miner...

Determine about the Secondary minerals Secondary minerals are formed under conditions of temperature and pressure found at the  Earth's surface by the weathering of pre-existi

Explain about the calcium and hypertension, Explain about the Calcium and H...

Explain about the Calcium and Hypertension? Chronic inadequate intake of calcium may play some role in etiologies of hypertension. Calcium deficiency has been linked to hyperte

What are prions? define concerns of food safety, Normal 0 false...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd