Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Class of mollusca - monoplacophora, Class of Mollusca - Monoplacophora ...

Class of Mollusca - Monoplacophora Bilaterally symmetrical, with broad flat foot and single shell, mantle cavity has five to six pairs of gills; six pairs of nephridia of whi

Monounsaturated fatty acids required for dyslipidemia, Q. Monounsaturated f...

Q. Monounsaturated fatty acids required for dyslipidemia? Polyunsaturated fatty acids also have a cholesterol decreasing effect. Omega 3 fatty acids are excellent for the heart

Factors affecting water requirements of microorganisms, Q. Factors Affectin...

Q. Factors Affecting Water requirements of microorganisms? Factors that may affect aw requirements of microorganisms include: 1. Kind of solute employed to reduce the aw: F

Show the major proteins that constitute the sarcomere, Q. What are the majo...

Q. What are the major proteins that constitute the sarcomere? What is the function of those molecules in the muscle cells? In the sarcomere there are organized myosin and actin

Hydropericardium-hepatitis syndrome, H yd r o p e r i c a r d i...

H yd r o p e r i c a r d i um - h e pa titi s syndrome Also known as 'Angara disease' or 'leachy heart disease', this complex illness of poultry spread rapid

Effect on microbial ecology and food spoilage of ph, Q. Effect on Microbial...

Q. Effect on Microbial Ecology and Food Spoilage of pH? The acidity of a product plays an important role in deciding the type microflora present in food and the rate and type o

International organization and zoonoses, International organization and zoo...

International organization and zoonoses In 1962, the Food and Agriculture Organization and the World Health Organization created the Codex Alimentarius Commission (Codex) to e

Define advantages of direct microscopic count, Define Advantages of Direct ...

Define Advantages of Direct Microscopic Count? 1. It is easy, inexpensive and relatively quick method. 2. It gives information regarding size and morphology of microorganism

State normal physiological saline, A complete motor neuron is deleted from ...

A complete motor neuron is deleted from a frog and placed in a large volume of normal physiological saline.  The neuron is healthy; it has a stable resting voltage of -70 mill

Primary tubercle, Primary  Tubercle  (Ghon Tubercle): When  an  indiv...

Primary  Tubercle  (Ghon Tubercle): When  an  individual with  no  previous exposure  to  tuberclosis inhale a sufficient number of  tubercle bacilli into the alveoli, tubercu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd