Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Effects of cardiac output and hormones, Excluding the effects of cardiac ou...

Excluding the effects of cardiac output and hormones, describe the other factors that may affect blood pressure and blood flow in a middle-aged man who is exercising in an aerobics

Define basic instruments used in biochemical laboratories, Basic Instrument...

Basic Instruments /Equipment Used In biochemical laboratories and important Working tips The first practical in this manual will orient you to the various equipment, apparatuses

What do you mean by platyhelminth phylum, Q What are the most tremendous kn...

Q What are the most tremendous known representatives of the platyhelminth phylum? The most popular representatives of the platyhelminthes are worms that cause human diseases, l

Define central nervous system, Q. Define Central nervous system? The ne...

Q. Define Central nervous system? The nervous system begins as a simple tube during embryonic development (then anterior part expands and also ventricles are formed).  Foreb

What are the flat bones and the long bones, What are the flat bones and the...

What are the flat bones and the long bones? The major bones of the body may be divided as flat or long bones (there are bones not classified into these categories). Such as fla

Why the av node of a mammalian heart is destroyed, The AV node of a mammali...

The AV node of a mammalian heart is destroyed. A. A depolarization in a cell in the left atrium will cause a depolarization of a cell in the left ventricle. B. The contracti

Define mineral trioxide aggregate - root end filling, Define Mineral Trioxi...

Define Mineral Trioxide Aggregate - Materials for Root End Filling Widely used Favorable biocompatibility Superior sealing qualities when compared to S-EBA and am

Can you explain pneumothorax, Q. Can you explain Pneumothorax? Air in t...

Q. Can you explain Pneumothorax? Air in the pleural cavity manifests in a number of ways on the CXR, depending on the volume of air and position of the patient. The typical fin

Define the effect of dietary fibre on satiety, Define the effect of Dietary...

Define the effect of Dietary fibre on Satiety? Satiety: Several investigators have speculated that ingestion of a high fibre food induces a feeling of satiety, reduces meal siz

Clinical features - rheumatic fever, The American Heart Association (AHA) h...

The American Heart Association (AHA) has recommended the revised Jones Criteria as a guide for ARF diagnosis. The same have been approved by WHO study group for the diagnosis of in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd