Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Sieve tubes, What are sieve tubes suited for translocation of food? a.bord...

What are sieve tubes suited for translocation of food? a.bordered pits b.no end walls c.bordered lumen and perforated cross walls D.no protoplasm

Define starch - carbohydrates, Define Starch - Carbohydrates? Starch is...

Define Starch - Carbohydrates? Starch is the major form of stored carbohydrate in plants. Starch, as you may already know, is composed of a mixture of two substances: amylose,

Non-striated or smooth muscles, NONSTRIATED (= SMOOTH) MUSCLES - Non...

NONSTRIATED (= SMOOTH) MUSCLES - Non-striated muscles are found in the posterior part of oesophagus, stomach, intestine, lungs, urinogenital tract, urinary bladder, blood ve

Why is it hard to produce vaccines against dengue and aids, Q. Why is it di...

Q. Why is it difficult to produce efficient vaccines against a viral infection like dengue and AIDS? It is difficult to make vaccines for dengue because there are four differen

Molluscus - hormones in growth and reproduction, Molluscus - Hormones in Gr...

Molluscus - Hormones in Growth and Reproduction We know quite well about the endocrine mechanism of growth and reproduction in the freshwater snail Lymnaea stagnalis. This is

Flower - plant growth substances, Flower - Plant Growth Substances Flo...

Flower - Plant Growth Substances Floral initiation is a dramatic event involving a total changeover of the character and developmental pattern of the meristem. The stimulus ca

Power plants of the aerobic cells, Why can mitochondria be considered the p...

Why can mitochondria be considered the power plants of the aerobic cells? Ans) Mitochondria are the "power plants" of aerobic cells due to within them the final stages of the ce

What are the bacteriophages, Q What are the bacteriophages? Bacteriopha...

Q What are the bacteriophages? Bacteriophages are viruses specialized in parasitism of bacteria. They are used in genetic engineering as molecular cloning vehicles to include r

Fossils formed by organisms that resemble modern, The oldest fossils look a...

The oldest fossils look as though they were formed by organisms that resemble modern?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd