Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

How can coacervates be formed of phospholipids, How can coacervates be form...

How can coacervates be formed of phospholipids or polypeptides? Phospholipids are amphipathic molecules, i.e., they present a polar portion and a nonpolar portion. In contact w

What are the factors affecting osseointegration, Factors Affecting Osseoint...

Factors Affecting Osseointegration a) Biocompatibility of the material b) Surgical technique- c) Bone density - d) Occlusal load e) Infection f) Implant surface

Define prevention of iron deficiency, Define Prevention of Iron Deficiency?...

Define Prevention of Iron Deficiency? Iron deficiency anaemia accounts for approximately one-half or more of all the anaemia's seen worldwide. Iron deficiency without anaemia a

Explain the various types of protein structure, Explain the various types o...

Explain the various types of Protein Structure? Protein Structure :  The structure of proteins can be examined at four levels of increasing complexity, with the primary struc

What are the autotrophic beings called, In the ecological study of food int...

In the ecological study of food interactions, what are the autotrophic beings called? In the Ecology autotrophic beings are called as producers because they synthesize the orga

Ovule, Ovule The ovule, also known as the megasporangium is the forer...

Ovule The ovule, also known as the megasporangium is the forerunner of the seed. It consists of a central mound of tissue called the nucellus, which is enveloped by one or tw

Define epithelial tissue, Epithelial tissue the cells in animals which are...

Epithelial tissue the cells in animals which are closely packed in either single or multiple layers, and which cover internal and external surfaces both of the animal body. It is

Explain the term viruses, Normal 0 false false false EN...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Define about the environmental deficiency of iodine, Define about the Envir...

Define about the Environmental deficiency of iodine? The ultimate causative Factor is deficient intake of iodine. Iodine occurs in soil and sea as iodide, the ions of which arc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd