Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Admission information - nursing, Admission Information   The physician ...

Admission Information   The physician and nurses are the primary source of facts concerning the purpose of therapeutic plan and expected outcome of hospitalization. The informa

Obelia, what is obelia write its external feature and mainly economic impo...

what is obelia write its external feature and mainly economic important

Respiration, How does Respiration in animals

How does Respiration in animals

Medical and first aid, Medical and First Aid - Factors Affecting Occupation...

Medical and First Aid - Factors Affecting Occupational Health Depending on the type of work the industry must make arrangement for readily available medical facility. A nearby

The trp repressor , In the lack of tryptophan shown in the figure, a trp r...

In the lack of tryptophan shown in the figure, a trp repressor protein encoded by a split operon, trpR that is synthesized and forms a dimer.  Furthermore, this is inactive and so

Explain the spectrophotometer or colorimeter, Explain the Spectrophotometer...

Explain the Spectrophotometer or Colorimete? These can be used to measure the microbial growth or can be used in various microbial and biochemical assays. Figure illustrates t

What are seed gums, What are seed gums? What are its characteristic propert...

What are seed gums? What are its characteristic properties? Seed gums are a group of polysaccharides with the following properties: light to dark cream colored amorphou

What is the climax stage of an ecological succession, Q. What is the climax...

Q. What is the climax stage of an ecological succession? The climax stage is the stage of an ecological succession in which the community of an ecosystem turn into stable and d

Contagious bovine pleuropneumonia, C o n t a g i o u s bovine pleu...

C o n t a g i o u s bovine pleuropneumonia It is a disease of bovines characterized by high rise of body temperature and difficulty in respiration. E t iolo

Determine the functions of human body system, Determine the function of hum...

Determine the function of human body system Human body has different systems to perform different functions. A system consists of organs to perform a particular set of function

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd