Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Stratification, Stratification On the basis of the variation in air tem...

Stratification On the basis of the variation in air temperature. the atmosphere has been divided vertically into four layers: (figure shown below) thee troposphere, stratospG,

Explain temperate deciduous forests-taiga and tundra, Explain Temperate Dec...

Explain Temperate Deciduous Forests-Taiga and tundra? Temperate deciduous forests are typified by the type of forests predominantly found in the eastern and northeastern Unit

How many respectively are genotypical and phenotypical forms, In F2 generat...

In F2 generation of a hybridization for a given trait conditioned by a pair of alleles T and t, according to Mendel's first law what are the genotypes of each phenotypical form? An

What is the meaning of darwins expression, What is the meaning of Darwin's ...

What is the meaning of Darwin's expression "descent with modification"? Descent with modification refers to natural selection. Descent with modification refers to evolutionary chan

How an electron acceptor becomes nadh, If NAD+ is an electron acceptor that...

If NAD+ is an electron acceptor that becomes NADH and the NADH then donates a proton to ATPase, shouldn't NADH now become NAD- since it lost it's proton but still has the electron?

Structure and function of triacylglycerols , Triacylglycerols  which is als...

Triacylglycerols  which is also  known as  triglycerides or fats  consist  of  three  fatty  acid chains  esterified  to  a  glycerol  backbone.  Simple  triacylglycerols   have  t

Zoology , canal system in porifera

canal system in porifera

Describe infectious agents, Describe Infectious Agents ? Recently there...

Describe Infectious Agents ? Recently there has been increasing interest in the relation between chronic low grade systemic inflammation and mortality of coronary artery diseas

Human skeleton, HUMAN SKELETON - Hard supportive or protective elements...

HUMAN SKELETON - Hard supportive or protective elements of the animal body form the skeleton system. It's study in osteology. EXOSKELETO N - Present outside skin. It

Dietary management for short bowel syndrome, Q. Dietary Management for shor...

Q. Dietary Management for short bowel syndrome? It must be evident from the symptoms listed above that the disease results in reduced food intake, impaired absorption and hence

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd