Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Rejection reaction, Rejection Reaction For rejection reaction the phys...

Rejection Reaction For rejection reaction the physiological and biochemical processes are set in the pistil by the recognition reaction specific to the type of pollen that lan

What is interdental threaded cleaners, What is Interdental Threaded Cleaner...

What is Interdental Threaded Cleaners The use of floss is recommended around any implant-supported bridge, crown, or bar. Instruct the patient to wrap the floss around the impl

Explain the nerve centres which control the body temperature, The nerve cen...

The nerve centres which control the body temperature and the urge for eating recontained in: 1.  Hypothalamus 2. Pons 3. Cerebellum 4. Thalamus Hypothalamus is the

What is a community, What is a community? What is the difference between th...

What is a community? What is the difference between the concepts of community and population? A community is a set of the populations of living beings that live in the same reg

Determine the floor of nose and nasal spine, Floor of nose and Nasal spine ...

Floor of nose and Nasal spine Although variable, the nasal floor is typically situated 5-9mm below the level of inferior turbinates. The inferior piriform rim is used as a guid

Explain the role in insulin formation, Explain the Role in Insulin Formatio...

Explain the Role in Insulin Formation? You are aware that insulin receptors are present in many cells with their concentration being highest in adipocytes (cells present in adi

Types of neurons, TYPE S OF NEURONS - The neurons are classified on th...

TYPE S OF NEURONS - The neurons are classified on the basis of their structure and function. (A) On the basis of the structure, the neurons are of four types.

What is the formula is to determinate such number?, Considering the indepen...

Considering the independent segregation of all factors, how many types of gametes does a VvXXWwYyzz individual produce? What is the formula is to determinate such number? The m

Explain the hydrophobic grid membrane filter (hgmf), Explain the Hydrophobi...

Explain the Hydrophobic Grid Membrane Filter (HGMF)? It is also used to enumerate microorganisms from a variety of food samples. By using this method, organisms as few as 10 ce

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd