Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Determine the failures at during abutment connection, During Abutment Conne...

During Abutment Connection Implants should be tested for the presence of any mobility and the radiographs should be taken. These radiographs are very vital as they serve as the

Cleavage of fructose-1, Cleavage of fructose-1,6-bisphosphate Aldolase...

Cleavage of fructose-1,6-bisphosphate Aldolase cleaves  fructose-1, 6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde-3-phosphate in the reversible reaction.

What concerns should alek''s healthcare, Nine-year-old Alek is admitted to ...

Nine-year-old Alek is admitted to the emergency room with a closed fracture of the right femur. Radiography revels that the fracture crosses the distal epiphyseal plate. What conce

Phanerophytes - classes of life form, Phanerophytes - Classes of Life Form ...

Phanerophytes - Classes of Life Form The perennating buds in this case are present on erect, negatively geotropic shoots, much above the ground. These buds are naked or least

What is diagnostic wax up, Diagnostic Wax Up A diagnostic wax up should...

Diagnostic Wax Up A diagnostic wax up should be done by you or got done from a laboratory for each and every case regardless of the extent or difficulty level of the case as it

What is procambium, What is Procambium? The procambium produces cells...

What is Procambium? The procambium produces cells that eventually become the vascular tissues xylem and phloem. These primary vascular tissues form a core in the center of th

Implantation - pre-embryonic development, Implantation - Pre-Embryonic Deve...

Implantation - Pre-Embryonic Development After entering the uterus and formation of ICM, the blastocyst starts to embed in the endometrium of the uterine wall. By one week aft

Respiration would cease even oxygen and food were supplied, If a tissue was...

If a tissue was heated to 65ºC for 10 minutes, respiration would cease even if oxygen and food were supplied. Why is this? A temperature of 65°C for 10 minutes would denature

Of which type of tissue is the heart made, Of which type of tissue is the h...

Of which type of tissue is the heart made? How is this tissue oxygenated and nutrified? The heart is made of striated cardiac muscle tissue. The heart muscle is known as the my

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd