Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Human population-historical overview, Human Population-Historical Overview ...

Human Population-Historical Overview Throughout history, the human population has been quite small. It has grown relatively slowly and even experienced occasional declines. Fi

Describe wide split s in details, Describe Wide Split S in details? It ...

Describe Wide Split S in details? It is a persistent split that widens on inspiration to at least 60 msec. May be due to delay in P, e.g. RBBB or due to early A, as occasionall

Nutritional changes and requirement - geriatric nutrition, Explain the Nutr...

Explain the Nutritional Changes and Requirement - Geriatric Nutrition? Nutrition is affected in two way -due to the changes in physiological function with  aging having effect

Categorisation of major brain functions, Categorisation of Major Brain Func...

Categorisation of Major Brain Functions As a framework, the major brain functions typically divided into modalities and domains. The major modalities are motor function and th

Important aspect of dietary management during hypertension, Q. Important as...

Q. Important aspect of dietary management during hypertension? Most important aspect of dietary management i.e. the intake of minerals and electrolytes, which are closely assoc

Pathophysiology and medical mangement of acute renal failure, Pathophysiolo...

Pathophysiology of Acute Renal Failure It is usually reversible but the deviation of physiologic function can be extreme and mortality in pediatric age group remains high. Th

Molecular hydrogen, Molecular Hydrogen N 2 -fixing organisms also prod...

Molecular Hydrogen N 2 -fixing organisms also produce a membrane bound enzyme called uptake-hydrogenase under N 2 -fixing condition. The physiological significance of the pres

Campylobacter infection, C ampylobacter infection C a mpylobacter ...

C ampylobacter infection C a mpylobacter jejuni is the commonest species found in poultry. These are delicate organisms that may not survive outside the host unless prot

Imaginal discs, Imaginal Discs In the holometabolous larva, there are ...

Imaginal Discs In the holometabolous larva, there are two cell populations: (1) The larval cells that are used for the larval structures and (2) The imaginal disc and th

Explain the determination and application of vitamin e, Explain the Determi...

Explain the Determination of Vitamin E DL-α-Tocopherol is oxidized with ferric chloride and the ferrous ions formed are measured colorimetrially with the aid of α,α'-dipyridyl

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd