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Explain the Turbidity Measurement?
Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).
Summer Stratification - Thermal stratification Thermal stratification is fairly pronounced during the summer seasons in most lakes of the temperate (cold) regions but is rare
DIFFERENCE BETWEEN LIVING AND NON-LIVING BEINGS - S .No. C h a ra cte r L i v i n g be ings N o n - l i v i n g be
where does kreb cycle takes place
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Q. Illustrate the tyndallisation? John Tyndall devised a process of sterilisation by steaming for a few minutes at 100 o C on 3-4 successive operations separated by 24 hour in
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How are amino acids brought to the cellular site where translation takes place? What is an anticodon? Amino acids are brought to ribosomes by RNA molecules called as transfer R
WHICH SOIL WILL BE FRUIFUL FOR GROWING LENTILS AND OTHER PULSES
Define Distribution of Food Products - Public Nutrition? We learnt earlier that we have buffer stocks of food grains in our country. These stocks do help to combat acute transi
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