Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Explain hepatitis c, Hepatitis C It provides sustained viral responses ...

Hepatitis C It provides sustained viral responses (SVRs) of 54% to 63%. When used as monotherapy, peginterferons once weekly are more effective than standard interferon 3 times

Poultry and duck diseases-marek''s disease, Marek's disease Marek's dis...

Marek's disease Marek's disease is a lymphoproliferative disease of chicken, quails, bantams and other free-flying birds. It is caused by the Gallid Herpesvirus 2 of subfamily

Determine teenager mindset and the eating disorders, Determine Teenager min...

Determine Teenager mindset and the eating disorders? Self image is an important determinant of weight and figure. Mostly girls under eat as already mentioned earlier, in order

Types of endoplasmic reticulum, TYPES E.R. consists of two types -     ...

TYPES E.R. consists of two types -        1. Rough E.R.                2. Smooth E.R.   R o ugh E.R.   S m o o th E.R.

What is pcr and how does pcr works, What is PCR? How does PCR works? Th...

What is PCR? How does PCR works? The PCR, polymerase chain reaction, is a method to synthesize many copies of specific regions of a DNA molecule known as target-regions. Its in

Is this the same thing as tonicity, These labs discuss the principle of osm...

These labs discuss the principle of osmolarity, which is defined as the total number of solute particles in 1 L of solution. Is this the same thing as tonicity? Explain your answer

Describe the origin of muscle cramps and pains, Q. How can the knowledge ab...

Q. How can the knowledge about fermentation describe the origin of muscle cramps and pains after intense physical exertion? A typical fermentation process due to oxygen scarcit

Define regents for determination of inorganic phosphorus, Define Regents fo...

Define Regents for Determination of Inorganic Phosphorus? 1. Trichloroacetic acid (10%) - Dissolve 100 g of high grade crystalline acid in distilled water and dilute to 1 Litre

Why is it important for chromosomes to be condensed, Why is it important fo...

Why is it important for chromosomes to be condensed during mitosis and decondensed during interphase? During mitosis the major problem to be solved is the correct separation of

An a=t mispairing leads to an a=c substitution, An A=T mispairing leads to ...

An A=T mispairing leads to an A=C substitution. The other DNA helix will contain a(n) __ pair. a. A=C b. A=T c. G=C d. B=Q e. T=T Can you also explain it please so if I'm ask

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd