Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Eggs of hen, EGG S OF HEN It is a meiolecithal egg which is oval. T...

EGG S OF HEN It is a meiolecithal egg which is oval. The animal pole is found in the form of a small germinal disc. The vegital pole has two types of yolk viz. White yol

#title.zoology., #question.why obelia is considered of special interest in ...

#question.why obelia is considered of special interest in zoology.

Glucose rehydration therapy, The movement  of Na + and glucose  from the l...

The movement  of Na + and glucose  from the lumen  of the intestine  across  the epithelial  cell to the blood  sets up a dissimilarity  in osmotic  pressure  across  the cell. As

Biological responses controlled by phytochrome, Biological Responses Contro...

Biological Responses Controlled by Phytochrome Phytochrome responses are those which are controlled reversibly by red and far-red light. These can be broadly categorised as:

What are some things that have algae in them, What are some things that hav...

What are some things that have algae in them? Yeast is considered Algae.

Zoology, living and non living

living and non living

What is spectroscopy, Question Write a short note on the following ...

Question Write a short note on the following 1 Simple diffusion 2 Viscosity 3 What is spectroscopy? List and explain types of spectroscopy 4 With a neat diagram,

Evolution of heart in higher animals, Evolution of heart in higher animals ...

Evolution of heart in higher animals The circulatory system in higher animals has undergone several changes during evolution. The heart has become totally muscular. Th

Sarcodina, general character of class sarcodina

general character of class sarcodina

Freshwater animals, Freshwater Animals In Figure you have seen that th...

Freshwater Animals In Figure you have seen that the body fluids of freshwater animals are hyperosmotic to their aqueous surroundings. This results in two-kinds of osmotic prob

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd