Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Why biologists use comparative method, Normal 0 false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Definition of diabetes mellitus, Q. DEFINITION OF DIABETES MELLITUS? Th...

Q. DEFINITION OF DIABETES MELLITUS? The word "diabetes" is derived from the Greek word meaning "a siphon". The patients of diabetes had polyuria (passing excessive urine) and "

Nervous system, a) Produce annotated diagrams that illustrate how nerve imp...

a) Produce annotated diagrams that illustrate how nerve impulses travel i) along a neurone ii) across a synapse.

Phylogeny and evolution, While studying evolution, a student comes across a...

While studying evolution, a student comes across a cladogram that includes clades like amphibia, reptilia, aves, and mammalia. What must be the basal clade?

Name the various test batteries, Name the various Test batteries Test ...

Name the various Test batteries Test batteries typically assess the following domains: General cognitive ability Language ability Visuoperceptual and constructi

How much fluids should be taken for management of obesity, How much Fluids ...

How much Fluids should be taken for management of obesity? Liberal amounts of water and zero/low calorie fluids may be included in the diet. It may be helpful to have a glass o

Cycles of matter, describe the path of nitrogen through its biogenochemicsa...

describe the path of nitrogen through its biogenochemicsal cycle

Why dairy or milk product are important for human body, Why Dairy or milk p...

Why Dairy or milk product are important for human body? This first food of mammals is rich in body-building proteins and bone-forming calcium, besides being the only source of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd