Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Define limitations for standard or total plate counts method, Define Limita...

Define Limitations for Standard or Total Plate Counts Method? This method has certain limitations - 1. Microbial count depends on the type of media, incubation temperature,

Impediments to infuse biology - lack of trained people, Define Impediments ...

Define Impediments to infuse biology - Lack of trained people? At present, biologists as a rule have not been trained sufficiently in quantitative approaches. A heavy dose of m

Bracketed keys to identifying plants and animals, Q. Bracketed Keys to iden...

Q. Bracketed Keys to identifying plants and animals? In bracketed keys two couplet leads of the same rank are bracketed together. The alternative clues or couplets are written

Historical review of developmental biology, HISTORICA L REVIEW Anox...

HISTORICA L REVIEW Anoximander (600 B.C.) was the first western philospher who proposed the idea that "living creatures arose from the moist element". Empedocles (500 B.

Define sympathetic and the parasympathetic neural actions, Q. What is the a...

Q. What is the antagonism between the sympathetic and the parasympathetic neural actions? In general the action of the parasympathetic and the sympathetic are antagonistic that

How is cancer usually treated, Q. How is cancer usually treated? If the...

Q. How is cancer usually treated? If the cancer is in its initial stage cure is often done by surgical removal of the neoplastic tissue. The Cancers already disseminated are of

The glomerular filtrate in comparison to the blood, What is the main transf...

What is the main transformation presented by the glomerular filtrate in comparison to the blood? Glomerular filtrate is the name given to the plasma after it has passed the glo

Define sample preparation for haemoglobin content in blood, Define Sample P...

Define Sample Preparation for Haemoglobin Content in Blood Wipe the index finger or middle finger with a cotton swab soaked in spirit. Prick the finger with a lancet op

Characteristics of genetically modified foods, Normal 0 false ...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Define cercaria larva, Define Cercaria Larva? Cercaria  larva is the f...

Define Cercaria Larva? Cercaria  larva is the fourth  larval stage  in  the life-cycle of F, hepatica. It is a free living stage produced  by  the  redia  larva. 1. It has

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd