Explain the turbidity measurement, Biology

Assignment Help:

Explain the Turbidity Measurement?

Presence of sufficient number of microbes makes the liquid medium turbid. Turbidity also increases with the multiplication of microbes. Estimation of turbidity can be used both for the quantitation of microbes and monitoring microbial growth. The technique depends on the fact that cells scatter the light striking them and the amount of scattering is directly proportional to the biomass of cells present and indirectly to the cell number. The scattering of light can be measured by spectrophotometer or photometer. In the instrument, a beam of light is passed through a cell suspension and the amount of unscattered light is detected by a light sensitive detector. Photometer uses a simple filter to generate incident light whereas in spectrophotometer, prism is used instead of a filter. Commonly used wavelengths for bacteria are 540 nm, 600 nm and 660 nm. More the number of microbes in the suspension, less is the light reaching to the detector and read as percent transmission or absorbance (OD, optical density, a logarithmic expression).


Related Discussions:- Explain the turbidity measurement

Define dark field microscope, Define Dark Field Microscope In this micr...

Define Dark Field Microscope In this microscope, condenser system is modified, so that only reflected and refracted light by specimen forms an image. Here, the object is bright

Avian (fowl cholera), Avian (fowl cholera) Fowl cholera is a contagious...

Avian (fowl cholera) Fowl cholera is a contagious septicaemic disease of almost all classes of fowl. The causal agent is Pasteurella multocida.Serotypes A:1 and A:3 are usually

Supply of animals in lab, Supply of Animals: Lab animals must be obtained ...

Supply of Animals: Lab animals must be obtained from accredited dealers and by accredited dealers, we mean suppliers in the business of supplying animals for lab use, and not from

Formation of double bonds, In eukaryotes  the SER has enzymes  able to begi...

In eukaryotes  the SER has enzymes  able to begin  double  bonds into fatty acyl CoA molecules  in an oxidation  reaction  which uses molecular  oxygen.  This reaction is catalyzed

Pyramid of numbers - ecological pyramids, Pyramid of Numbers - Ecological P...

Pyramid of Numbers - Ecological Pyramids A graphic representation of the total number of individuals of different species belonging to each trophic level in an ecosystem is kn

Types of fertilization, TYPES OF FERTILIZATION - On the basis of site o...

TYPES OF FERTILIZATION - On the basis of site of fertilization, it is of two types - 1. External fertilization        2. Internal fertilization 1 .       EXTERNAL FERTILIZ

Essentials of Human Diseases and Conditions, How do pathological conditions...

How do pathological conditions affect homeostasis of your body?

How much proteins should be taken for management of obesity, How much Prote...

How much Protein should be taken for Management of Obesity? Adequate amount of proteins should be included in the diet to ensure proper metabolism and prevent weakness which is

State what is genetic engineering, State what is Genetic engineering Ge...

State what is Genetic engineering Genetically engineered plant-incorporated protectants are created by a process that utilizes  several dissimilar modern scientific techniques

Can you explain nerves, Q. What are nerves? Axons extend all through th...

Q. What are nerves? Axons extend all through the body inside nerves. Nerves are axon-containing structures presenting many axons and covered by connective tissue. The nerves co

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd