Explain the transfer from liquid medium to solid media, Biology

Assignment Help:

Explain the Transfer from liquid medium to solid media?

Here the steps involved include:

1. Inoculating loop is sterilized by incineration and allowed to cool, as described earlier.

2. Liquid culture tube is held in left hand and cotton plug or cap is removed with the little finger of the right hand that holds the sterile inoculating loop.

3. Neck of the tube is flamed briefly and a loopful of culture is taken by inoculating loop for inoculating agar plate or slants.

4. Cap or Cotton plug is replaced on the tube after reflaming the mouth.

5. Agar plate is held in the palm of the left hand near the flame and lid is opened slightly with the thumb. The agar plate is inoculated either in the centre by the culture on the inoculating loop or by the streak method and lid is replaced on the plate.

6. For agar slants, cotton plug or cap is removed from the slant tube. The neck is flamed and then slant is inoculated by inserting and drawing inoculating loop in a slant tube lightly over hardened surface in a zig-zag/straight line. The tube neck is reflamed and cap is replaced. For deep agar tubes, inoculation is done with sterilized inoculating needle. It is inserted straight into the agar upto the bottom (stab inoculation).


Related Discussions:- Explain the transfer from liquid medium to solid media

Bovine rotavirus diarrhoea, Bovine rotavirus diarrhoea The bovine rota...

Bovine rotavirus diarrhoea The bovine rotavirus is a RNA virus with 11 segments of double stranded RNA belonging to the genus Rotavirus in the family Reoviridae. Rotaviruses c

What is defense mechanism, What is Defense mechanism Defense mechanism...

What is Defense mechanism Defense mechanism  relate to the body's ways to protect itself from any infection or a disease

Define precautions for simple staining of bacterial cultures, Define Precau...

Define Precautions for Simple Staining of Bacterial Cultures? 1. Heat fix all the bacterial smears. 2. Use clean, grease free glass slides. 3. Wash the stained slide gent

The denver development screening test (ddst), THE DENVER DEVELOPMENT SCREEN...

THE DENVER DEVELOPMENT SCREENING TEST (DDST) The Denver Development Screening Test is a standardized test to assess gross motor, fine motor, language and social development

Why to dilute this stock to give the desired concentration, You are working...

You are working on a protocol in lab that requires 10ml of a 5% saline solution for a particular step. All you have in your lab is a 20% saline stock solution. How can you dilute t

Rituals , Rituals: The social life of  the earliest human groups or tri...

Rituals: The social life of  the earliest human groups or tribes revolved around food gathering. To begin with, they must have collected anything they could eat-seeds,  nuts, f

Nutrition, vulture undergo which type of nutrition

vulture undergo which type of nutrition

Neutralize acid rain because water is buffered, Select all that are true or...

Select all that are true or correct regarding a buffer: alternately donating and accepting ions a set of chemicals that work together to resist pH change often a weak acid or base

Tonicity and the plant cells., Tonicity and the plant cells. Complete the ...

Tonicity and the plant cells. Complete the experiment and answer the questions. Table 7.3: Potato type: Potato A: Beginning displacement (ml) = Ending displacem

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd