Explain the transfer from liquid medium to solid media, Biology

Assignment Help:

Explain the Transfer from liquid medium to solid media?

Here the steps involved include:

1. Inoculating loop is sterilized by incineration and allowed to cool, as described earlier.

2. Liquid culture tube is held in left hand and cotton plug or cap is removed with the little finger of the right hand that holds the sterile inoculating loop.

3. Neck of the tube is flamed briefly and a loopful of culture is taken by inoculating loop for inoculating agar plate or slants.

4. Cap or Cotton plug is replaced on the tube after reflaming the mouth.

5. Agar plate is held in the palm of the left hand near the flame and lid is opened slightly with the thumb. The agar plate is inoculated either in the centre by the culture on the inoculating loop or by the streak method and lid is replaced on the plate.

6. For agar slants, cotton plug or cap is removed from the slant tube. The neck is flamed and then slant is inoculated by inserting and drawing inoculating loop in a slant tube lightly over hardened surface in a zig-zag/straight line. The tube neck is reflamed and cap is replaced. For deep agar tubes, inoculation is done with sterilized inoculating needle. It is inserted straight into the agar upto the bottom (stab inoculation).


Related Discussions:- Explain the transfer from liquid medium to solid media

Ureotelism - excretion, Normal 0 false false false EN-I...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What are the factors to an actual shift in the supply curve, What are the f...

What are the factors to an actual shift in the supply curve? Factors which cause an actual shift in the supply curve are described in below: When prices rise or fall this wo

Define criteria for assessment of niacin status, Define Criteria for Assess...

Define Criteria for Assessment of Niacin Status? Niacin status can be monitored by daily urinary excretion of methylated metabolites, especially the ratio of the 2-pyridone to

Which is better to grow plants in rock sand or soil, Explain which is bette...

Explain which is better to grow plants in Rock sand or soil? Ans) When we grew plants inside, with no wind, and the plants in the rocks grew superior to the plants that were gr

Myocardial hypertrophy and remodeling, The long term adaptive mechanisms in...

The long term adaptive mechanisms involve myocardial hypertrophy and remodeling which occurs slowly over weeks to months. The capacity of these adaptive mechanisms meant to sust

Light-environmental components, LIGHT All of us know that the sun is the ...

LIGHT All of us know that the sun is the ultimate source of energy for all activities in our biosphere. The electromagnetic radiations from the sun supply energy which warms up t

What is sustainability, What is sustainability? Sustainability is the a...

What is sustainability? Sustainability is the ability to meet human needsin a way that will allow a human population to survive indefinitely.

Typical components of a closed circulatory system, Q. What are the typical ...

Q. What are the typical components of a closed circulatory system? The typical components of the closed circulatory system are the blood vessels within which blood circulates c

Explain the direct microscopic counts (dmc), Explain the Direct Microscopic...

Explain the Direct Microscopic Counts (DMC) The process includes making a smear of food sample on a microscope slide followed by staining with an appropriate dye and viewing an

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd