Explain the transfer from liquid medium to solid media, Biology

Assignment Help:

Explain the Transfer from liquid medium to solid media?

Here the steps involved include:

1. Inoculating loop is sterilized by incineration and allowed to cool, as described earlier.

2. Liquid culture tube is held in left hand and cotton plug or cap is removed with the little finger of the right hand that holds the sterile inoculating loop.

3. Neck of the tube is flamed briefly and a loopful of culture is taken by inoculating loop for inoculating agar plate or slants.

4. Cap or Cotton plug is replaced on the tube after reflaming the mouth.

5. Agar plate is held in the palm of the left hand near the flame and lid is opened slightly with the thumb. The agar plate is inoculated either in the centre by the culture on the inoculating loop or by the streak method and lid is replaced on the plate.

6. For agar slants, cotton plug or cap is removed from the slant tube. The neck is flamed and then slant is inoculated by inserting and drawing inoculating loop in a slant tube lightly over hardened surface in a zig-zag/straight line. The tube neck is reflamed and cap is replaced. For deep agar tubes, inoculation is done with sterilized inoculating needle. It is inserted straight into the agar upto the bottom (stab inoculation).


Related Discussions:- Explain the transfer from liquid medium to solid media

Cows of identical genotype - polled condition, 1. A roan bull which is hete...

1. A roan bull which is heterozygous for the polled condition is mated to several cows of identical genotype to his. How many roan, polled animals should be produced out of 16?

Homologous proteins - amino acid sequence in fasta format, Please answer th...

Please answer the following question on Sequence Y: Protein databases 1. What sequence from which organism is this sequence most similar to? 2. Can you find any homo

Why are grasshopper is said to show male heterogamity, a) Why are grasshopp...

a) Why are grasshopper and Drosophila said to show male heterogamity? Describe. b) Define female heterogamity with the help of an example.

What is diarrhoea, What is diarrhoea? Diarrhoea is characterized by th...

What is diarrhoea? Diarrhoea is characterized by the frequent evacuation of liquid stools, usually exceeding 300 ml, accompanied by an excessive loss of fluids and electrolyte

Phylum arthropoda - classification of multicellular animals, Phylum Arthrop...

Phylum Arthropoda - Classification of Multicellular Animals Earlier you known how the coelom evolved and the body of the coelomates got segmented leading to evolution of segme

What is the difference between amnion and chorion, What is the difference b...

What is the difference between amnion and chorion? Amnion is the membrane that covers the embryo. Chorion is the membrane that covers the amnion, the yolk sac and the allantois

Processing grains and oil seeds for animal feeding, Processing grains and o...

Processing grains and oil seeds for animal feeding Simple processing of grains such as grinding facilitates the digestion particularly in cattle and buffaloes. Fine grinding of

Photosynthesis, explain the role of photophosphorylation and chemiosmosis i...

explain the role of photophosphorylation and chemiosmosis in photosynthesis

Signify the term - opercular gill, Signify the term - opercular gill. T...

Signify the term - opercular gill. The gill found in bony fish. Operculum is the outer covering of the gill. When it's drawn away from the body, it pulls water inside the bucca

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd