Explain the transfer from liquid medium to solid media, Biology

Assignment Help:

Explain the Transfer from liquid medium to solid media?

Here the steps involved include:

1. Inoculating loop is sterilized by incineration and allowed to cool, as described earlier.

2. Liquid culture tube is held in left hand and cotton plug or cap is removed with the little finger of the right hand that holds the sterile inoculating loop.

3. Neck of the tube is flamed briefly and a loopful of culture is taken by inoculating loop for inoculating agar plate or slants.

4. Cap or Cotton plug is replaced on the tube after reflaming the mouth.

5. Agar plate is held in the palm of the left hand near the flame and lid is opened slightly with the thumb. The agar plate is inoculated either in the centre by the culture on the inoculating loop or by the streak method and lid is replaced on the plate.

6. For agar slants, cotton plug or cap is removed from the slant tube. The neck is flamed and then slant is inoculated by inserting and drawing inoculating loop in a slant tube lightly over hardened surface in a zig-zag/straight line. The tube neck is reflamed and cap is replaced. For deep agar tubes, inoculation is done with sterilized inoculating needle. It is inserted straight into the agar upto the bottom (stab inoculation).


Related Discussions:- Explain the transfer from liquid medium to solid media

After the morula stage what is the next stage, Q. After the morula stage wh...

Q. After the morula stage what is the next stage? What is the morphological feature that defines this stage? In the blastula stage the compactness is lost and an internal cavit

Maintenance of the normal quantity of chromosomes, Q. Why is meiosis signif...

Q. Why is meiosis significant for the maintenance of the normal quantity of chromosomes of a species with sexual reproduction? A reduction to a half of the maximum normal quant

Explain congenital heart disease (chd), Explain Congenital Heart Disease (C...

Explain Congenital Heart Disease (CHD)? Congenital heart disease is an abnormality at birth in cardiac circulatory structure or function. Optimal management of CHD aims not mer

Assessment of diastdlic function, Q. Assessment of Diastdlic Function? ...

Q. Assessment of Diastdlic Function? Myocardial ischemia Clters diastolic function of left ventricle. The earliest abnormality to appear with prolonged ischemia is delayed my

Explain phylum ascomycetes, Phylum Ascomycetes 1) Sexual reproduction i...

Phylum Ascomycetes 1) Sexual reproduction is by conjugation and is followed by the formation of ascospores inside a sac called ascus. 2) The asci may be grouped together to

Telophase of karyokinesis, - The chromosomes at each pole uncoil and elonga...

- The chromosomes at each pole uncoil and elongate to form the chromatin. - A nucleolus reappears at each pole. - Spindle fibers and asters disappear and centrioles split. - A nu

Potential and excitation threshold for neurons, Q. How different are the co...

Q. How different are the concepts of action potential, resting potential and excitation threshold concerning neurons? Action potential is the maximum positive voltage level ach

Why networking in hospitals, WHY NETWORKING IN HOSPITALS Sharing of dat...

WHY NETWORKING IN HOSPITALS Sharing of data is essential, especially in any kind of hospital setting. For staff and doctors of a hospital, if they have details of patients rea

Explain about the plateau effect - energy balance, Explain about the platea...

Explain about the plateau effect - Energy Balance? You must have noticed that when people start following weight reducing diets, they lose weight rapidly in the beginning, then

Bacterial diseases-black disease, Black disease It is a fatal peracute...

Black disease It is a fatal peracute disease caused by Clostridium novyi Type B. It is associated with liver damage caused by liver fluke. The onset of the disease is rapid fo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd