Explain the transfer from liquid medium to solid media, Biology

Assignment Help:

Explain the Transfer from liquid medium to solid media?

Here the steps involved include:

1. Inoculating loop is sterilized by incineration and allowed to cool, as described earlier.

2. Liquid culture tube is held in left hand and cotton plug or cap is removed with the little finger of the right hand that holds the sterile inoculating loop.

3. Neck of the tube is flamed briefly and a loopful of culture is taken by inoculating loop for inoculating agar plate or slants.

4. Cap or Cotton plug is replaced on the tube after reflaming the mouth.

5. Agar plate is held in the palm of the left hand near the flame and lid is opened slightly with the thumb. The agar plate is inoculated either in the centre by the culture on the inoculating loop or by the streak method and lid is replaced on the plate.

6. For agar slants, cotton plug or cap is removed from the slant tube. The neck is flamed and then slant is inoculated by inserting and drawing inoculating loop in a slant tube lightly over hardened surface in a zig-zag/straight line. The tube neck is reflamed and cap is replaced. For deep agar tubes, inoculation is done with sterilized inoculating needle. It is inserted straight into the agar upto the bottom (stab inoculation).


Related Discussions:- Explain the transfer from liquid medium to solid media

Explain difference among essential and natural amino acids, What is the dif...

What is the difference among essential and natural amino acids? Essential amino acids are those that the organism is not able to synthesize and that require to be ingested by t

Define prevention of idd - iodized salt distribution, Define Prevention of ...

Define Prevention of IDD - Iodized salt distribution? Many approaches to reduce iodine deficiency has been formulated. Some of these are reviewed herewith: Iodized salt dist

Acute heart failure, Ac ute heart failure The acute heart failure is c...

Ac ute heart failure The acute heart failure is characterized by sudden loss of consciousness and falling with or without convulsions. The mucous membranes become pale followe

Explain about cerebral hemispheres, How is it structurally explained that t...

How is it structurally explained that the motor activity of the left side of the body is controlled by the right cerebral hemisphere and the motor activity of the right side of the

Define conjunctival xerosis - micronutrient deficiencies, Define Conjunctiv...

Define Conjunctival Xerosis - Micronutrient Deficiencies? Conjunctiva in nonnal children is bright white, smooth and glistening. Conjunctival xerosis is characterized by drynes

Explain the both sex-linked trait and a sex-influenced trait, Explain the d...

Explain the difference among a sex-linked trait and a sex-influenced trait. A sex-influenced trait is influenced by the presence of sex hormones and its genes are not situated

Define the dietary and lifestyle modifications - obesity, Define the Dietar...

Define the Dietary and Lifestyle Modifications - Obesity? The management of obesity basically comprises the following three- pronged approach. a) Dietary modifications b)

What are genotypes and phenotypes, The agouti locus in mice has three allel...

The agouti locus in mice has three alleles: A is recessive and confers a black coat, A is dominant to a and confers an agouti coat, and AY is dominant to both and confers a yellow

Is the bone marrow made of osseous tissue, Q. In which bones can bone marro...

Q. In which bones can bone marrow chiefly be found? Is the bone marrow made of osseous tissue? Bone marrow can mainly be found in the internal cavities of flat bones, like the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd