Explain the transfer from liquid medium to solid media, Biology

Assignment Help:

Explain the Transfer from liquid medium to solid media?

Here the steps involved include:

1. Inoculating loop is sterilized by incineration and allowed to cool, as described earlier.

2. Liquid culture tube is held in left hand and cotton plug or cap is removed with the little finger of the right hand that holds the sterile inoculating loop.

3. Neck of the tube is flamed briefly and a loopful of culture is taken by inoculating loop for inoculating agar plate or slants.

4. Cap or Cotton plug is replaced on the tube after reflaming the mouth.

5. Agar plate is held in the palm of the left hand near the flame and lid is opened slightly with the thumb. The agar plate is inoculated either in the centre by the culture on the inoculating loop or by the streak method and lid is replaced on the plate.

6. For agar slants, cotton plug or cap is removed from the slant tube. The neck is flamed and then slant is inoculated by inserting and drawing inoculating loop in a slant tube lightly over hardened surface in a zig-zag/straight line. The tube neck is reflamed and cap is replaced. For deep agar tubes, inoculation is done with sterilized inoculating needle. It is inserted straight into the agar upto the bottom (stab inoculation).


Related Discussions:- Explain the transfer from liquid medium to solid media

Subtropical rain forests - ecosystem, Subtropical rain forests - Ecosystem ...

Subtropical rain forests - Ecosystem In regions of fairly high rainfall but less temperature differences between winter and summer and broad-leaved evergreen subtropical biome

How to investigate mitral regurgitation by chest radiogram, Q. How to inves...

Q. How to investigate mitral regurgitation by Chest Radiogram? Enlarged left atrium is obvious on chest X-ray and it may occupy most of the cardiac silhouette in patients with

Nonsurgical retreatment will be the preferred choice, Nonsurgical retreatme...

Nonsurgical retreatment will be the preferred choice -Less invasive .. not cause problem to the pt. -Less traumatic postoperative .. most probably no post operative pain

How plant use day-night length to solve the paradox, How plants use the pat...

How plants use the pattern of changes in day/night length to solve the paradox that day length is equal twice a year. Plants possess the ability to balance the daily changes in

Explain fats in biological compound, Explain Fats in biological compound ? ...

Explain Fats in biological compound ? Fats :   Monoglycerides, diglycerides, and triglycerides are fats consisting of a backbone of the alcohol molecule glycerol, consisting of

Define the spray drying method, Define the Spray Drying Method? Spray d...

Define the Spray Drying Method? Spray drying is a unique drying process since it involves both particle formation and drying. It is most suitable for drying of liquid foods suc

Explai what is dna cloning , DNA Cloning in biology is the process of produ...

DNA Cloning in biology is the process of producing similar populations of genetically identical individuals which happens in nature when organisms like as insects, bacteria or plan

Explain shear pin model, Q. Explain Shear Pin Model? Shear Pin Model: I...

Q. Explain Shear Pin Model? Shear Pin Model: In this type, a shearing force is applied using a pin to shear the food Product. We are rupturing the element of the food product b

Indications for emergency surgery in ar, Indications for Emergency Surgery ...

Indications for Emergency Surgery in AR 1) Post balloon valvotomy AR with haemodynamic compromise 2) Acute thrombosis of prosthetic valve, not responding to thrombolysis

What is colloids and suspensions , What is Colloids and Suspensions ? ...

What is Colloids and Suspensions ? Colloids and Suspensions :  Colloids and suspensions are mixtures in which larger particles replace solutes. Colloids, or colloidal suspe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd