Explain the transfer from liquid medium to solid media, Biology

Assignment Help:

Explain the Transfer from liquid medium to solid media?

Here the steps involved include:

1. Inoculating loop is sterilized by incineration and allowed to cool, as described earlier.

2. Liquid culture tube is held in left hand and cotton plug or cap is removed with the little finger of the right hand that holds the sterile inoculating loop.

3. Neck of the tube is flamed briefly and a loopful of culture is taken by inoculating loop for inoculating agar plate or slants.

4. Cap or Cotton plug is replaced on the tube after reflaming the mouth.

5. Agar plate is held in the palm of the left hand near the flame and lid is opened slightly with the thumb. The agar plate is inoculated either in the centre by the culture on the inoculating loop or by the streak method and lid is replaced on the plate.

6. For agar slants, cotton plug or cap is removed from the slant tube. The neck is flamed and then slant is inoculated by inserting and drawing inoculating loop in a slant tube lightly over hardened surface in a zig-zag/straight line. The tube neck is reflamed and cap is replaced. For deep agar tubes, inoculation is done with sterilized inoculating needle. It is inserted straight into the agar upto the bottom (stab inoculation).


Related Discussions:- Explain the transfer from liquid medium to solid media

Peak flow velocity, Peak Flow Velocity  fs  derived  from Doppler  shift by...

Peak Flow Velocity  fs  derived  from Doppler  shift by  rearranging Doppler equation: V = C/2 x ΔF/Fo Various  information can be obtained from spectral display

Of what substances is the nucleolus made, Q. Of what substances is the nucl...

Q. Of what substances is the nucleolus made? Is there a membrane around the nucleolus? Nucleolus is a region within the nucleus made of proteins and ribosomal RNA (rRNA). It is

Venous return, This is the amount of blood that is returned to the heart by...

This is the amount of blood that is returned to the heart by the veins. This is influenced by various factors. Inspiration decreases the pressure in the  central veins and thus inc

Fate of pyruvate, Pyruvate Pyruvate has three different  fates. Under a...

Pyruvate Pyruvate has three different  fates. Under aerobic conditions,  pyruvate enters mitochondriaand is converted to acetyl CoA. The acetyl CoA enters thecitric acid cycle.

Define the term behaviour change communication, Q. Define the term Behaviou...

Q. Define the term Behaviour Change Communication? Behaviour Change Communication (BCC) is an interactive process with communities to develop specific messages and methods usin

Animal cloning, Animal Cloning: When entire animals are obtained from s...

Animal Cloning: When entire animals are obtained from somatic cells of an animal, it is known as animal cloning. Cloning is usual in plants but in case of animals only some deg

Meaning of metabolism, Q. Meaning of Metabolism? Metabolism refers to c...

Q. Meaning of Metabolism? Metabolism refers to chemical changes which absorbed food undergoes within the body cells. Body metabolizes carbohydrate, fat and protein to supply en

Explain about the gel bands detection techniques, Explain about the Gel ban...

Explain about the Gel bands detection techniques? Gel bands, resulting from a gel electrophoretic separation, may be detected by staining, radioactive counting or immunoblottin

Heparinisation, Heparinisation Blood when allowed to flow out from ...

Heparinisation Blood when allowed to flow out from body to the circuit tubings can get clotted. To prevent this, heparinisation is done. Patient is administered 3 mg

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd