Explain the transfer from liquid medium to solid media, Biology

Assignment Help:

Explain the Transfer from liquid medium to solid media?

Here the steps involved include:

1. Inoculating loop is sterilized by incineration and allowed to cool, as described earlier.

2. Liquid culture tube is held in left hand and cotton plug or cap is removed with the little finger of the right hand that holds the sterile inoculating loop.

3. Neck of the tube is flamed briefly and a loopful of culture is taken by inoculating loop for inoculating agar plate or slants.

4. Cap or Cotton plug is replaced on the tube after reflaming the mouth.

5. Agar plate is held in the palm of the left hand near the flame and lid is opened slightly with the thumb. The agar plate is inoculated either in the centre by the culture on the inoculating loop or by the streak method and lid is replaced on the plate.

6. For agar slants, cotton plug or cap is removed from the slant tube. The neck is flamed and then slant is inoculated by inserting and drawing inoculating loop in a slant tube lightly over hardened surface in a zig-zag/straight line. The tube neck is reflamed and cap is replaced. For deep agar tubes, inoculation is done with sterilized inoculating needle. It is inserted straight into the agar upto the bottom (stab inoculation).


Related Discussions:- Explain the transfer from liquid medium to solid media

Degradation/destruction, How does degradation/destruction differ on differe...

How does degradation/destruction differ on different vegatation zoner or at different heights?

Scentific american article suggests some improvements, The human skeleton h...

The human skeleton has evolved from that of four-legged animals. Unfortunately, the adaptation is far from perfect; thus, our upright posture causes problems like backage and the k

Explain phylum sarcdina, Phylum Sarcdina 1) They move by means of pseud...

Phylum Sarcdina 1) They move by means of pseudopodia (ralse feet) or similar structures. 2) They feed heterotrophically by phagocytosis.   Some examples: Amoeba, Entamo

Aerobic respiration, Ask question end product of aerobic repiration ar...

Ask question end product of aerobic repiration are

What are the main biological functions of water, Q. What are the main biolo...

Q. What are the main biological functions of water? Ans. Water is the basic solvent for chemical reactions of living beings; it is the main means of substance transportati

Proteins of animal origin - fish, Proteins of Animal Origin - Fish Fish...

Proteins of Animal Origin - Fish Fish: The edible portion is skeletal muscles of the body. Even though the skeletal muscles of different animals are basically similar, fish spe

Why o-h bond is a polar covalent bond, Describe how electro negativity can ...

Describe how electro negativity can explain why a C-H bond is a no polar covalent bond, but a O-H bond is a polar covalent bond.

Terms of incubation time and the generation time, If the generation time (t...

If the generation time (t) is the incubation time (t) per generation (G), or t = t/G, rewrite the formula you derived in question 2 for bacterial population growth in terms of incu

Explain pasteurization, Explain Pasteurization The act or process of h...

Explain Pasteurization The act or process of heating a beverage or other food, to a specific temperature for a specific period of time in order to kill microorganisms that cou

Rejection reaction, Rejection Reaction For rejection reaction the phys...

Rejection Reaction For rejection reaction the physiological and biochemical processes are set in the pistil by the recognition reaction specific to the type of pollen that lan

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd