Explain the transfer from liquid medium to solid media, Biology

Assignment Help:

Explain the Transfer from liquid medium to solid media?

Here the steps involved include:

1. Inoculating loop is sterilized by incineration and allowed to cool, as described earlier.

2. Liquid culture tube is held in left hand and cotton plug or cap is removed with the little finger of the right hand that holds the sterile inoculating loop.

3. Neck of the tube is flamed briefly and a loopful of culture is taken by inoculating loop for inoculating agar plate or slants.

4. Cap or Cotton plug is replaced on the tube after reflaming the mouth.

5. Agar plate is held in the palm of the left hand near the flame and lid is opened slightly with the thumb. The agar plate is inoculated either in the centre by the culture on the inoculating loop or by the streak method and lid is replaced on the plate.

6. For agar slants, cotton plug or cap is removed from the slant tube. The neck is flamed and then slant is inoculated by inserting and drawing inoculating loop in a slant tube lightly over hardened surface in a zig-zag/straight line. The tube neck is reflamed and cap is replaced. For deep agar tubes, inoculation is done with sterilized inoculating needle. It is inserted straight into the agar upto the bottom (stab inoculation).


Related Discussions:- Explain the transfer from liquid medium to solid media

Differences between electron acceptors and electron carriers, What are the ...

What are the differences between electron acceptors and electron carriers? Give an example of each.

What are the main bryophyte groups, What are the main bryophyte groups? ...

What are the main bryophyte groups? The main bryophyte groups are the mosses, the liverworts and the hornworts. Image Diversity: mosses liverworts hornworts

What is nucleotides, What is Nucleotides? Nucleotides are the basic b...

What is Nucleotides? Nucleotides are the basic building blocks of nucleic acids. Each nucleotide is composed of a nitrogen base, a 5-carbon sugar called a pentose, and an ass

Describe briefly tyndall effect, Tyndall Effect One of the best ways to...

Tyndall Effect One of the best ways to distinguish a solution from a colloidal dispersion is to use a strong beam of intense light. As the beam passes through a colloidal dispe

What is vaso-constriction, a) What is 'vaso-constriction'? b) W...

a) What is 'vaso-constriction'? b) What are the effects of vaso-constriction in the skin?   a) Vaso-constriction is the reduce in diameter of small arterioles an

Blood protozoan and ricketsial diseases - trypanosomiasis, B l oo d Prot...

B l oo d Protozoan and Ricketsial Diseases T r y p a n o s o m i a si s It is also known as surra in equines and tibarsa in camel and results in intermitte

Fuel requirements - deforestation, Fuel Requirements - Deforestation T...

Fuel Requirements - Deforestation The increasing demand for fuel wood is one of the major factors leading to the degradation of the forest ecosystem. Fuel wood is of such majo

What possible combinations of chromosomes will be present, A diploid cell c...

A diploid cell contains three pairs of homologous chromosomes designated C1 and C2, M1 and M2, and S1 and S2; no crossing over occurs. What possible combinations of chromosomes

Multipotent scs-pulp tissue revascularization, Multipotent SCs: are cells ...

Multipotent SCs: are cells differentiated & form a number of other tissues, while their sources are not fetal tissue; umblical cord blood & postnatal stem cells including dental p

Explain about the phosphorus - macro minerals, Explain about the Phosphorus...

Explain about the Phosphorus - Macro Minerals? Phosphorus is the second most abundant element in the human body, comprising 30% of the total mineral content. An adult human bod

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd