Explain the transfer from liquid medium to solid media, Biology

Assignment Help:

Explain the Transfer from liquid medium to solid media?

Here the steps involved include:

1. Inoculating loop is sterilized by incineration and allowed to cool, as described earlier.

2. Liquid culture tube is held in left hand and cotton plug or cap is removed with the little finger of the right hand that holds the sterile inoculating loop.

3. Neck of the tube is flamed briefly and a loopful of culture is taken by inoculating loop for inoculating agar plate or slants.

4. Cap or Cotton plug is replaced on the tube after reflaming the mouth.

5. Agar plate is held in the palm of the left hand near the flame and lid is opened slightly with the thumb. The agar plate is inoculated either in the centre by the culture on the inoculating loop or by the streak method and lid is replaced on the plate.

6. For agar slants, cotton plug or cap is removed from the slant tube. The neck is flamed and then slant is inoculated by inserting and drawing inoculating loop in a slant tube lightly over hardened surface in a zig-zag/straight line. The tube neck is reflamed and cap is replaced. For deep agar tubes, inoculation is done with sterilized inoculating needle. It is inserted straight into the agar upto the bottom (stab inoculation).


Related Discussions:- Explain the transfer from liquid medium to solid media

Coelom, Why Chordates are Schizocoelic, while other deuterostomes are enter...

Why Chordates are Schizocoelic, while other deuterostomes are enterocoelic?

Puerperal psychosis and rape - psychiatric emergencies, Puerperal Psychosis...

Puerperal Psychosis: Child birth can precipitate schizophrenia, depression and mania. Management:  it  depends on  the nature of the symptoms. Danger  to  self and othcrs (inc

Transport of gases in blood, Transport of Gases in Blood We know that ...

Transport of Gases in Blood We know that two types of gas exchanges are constantly occurring in the animal body--one at the inter phase of the respiratory membrane and externa

Which molecule should be most radioactive, Yeast is cultured in the presenc...

Yeast is cultured in the presence of radioactive phosphate and the following biological molecules are purified. Which molecule should be most radioactive? A. An oligosaccharide B.

Rheumatic fever, Rheumatic fever is an immunologically mediated connective ...

Rheumatic fever is an immunologically mediated connective tissue disorder following throat infection with group-A streptococci (GAS). It is characterised by an inflammatory proces

Define structures in a vertebrate with a four-chambered, Which of the follo...

Which of the following structures in a vertebrate with a four-chambered heart would have blood with the highest oxygen concentration? And why?

What is the water cycle, What is the water cycle? The water cycle repre...

What is the water cycle? The water cycle represents the circulation and recycling of water in nature. Liquid water on the planet surface is heated by the sun and turns into

The digestive processes, Alimentary tract The alimentary tract and the ...

Alimentary tract The alimentary tract and the secretary organs associated with it are involved in the process of digestion. The sequence of events includes ingestion of food, d

Existence value of biodiversity, There may also be non-use existence values...

There may also be non-use existence values for components of biological diversity due to the value placed on biodiversity purely based on its continued existence, irrespective of w

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd