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Where would a predicted silent mutation have to be situated to actually result in a loss-of-function mutation (and potentially lead to the onset of disease)?
A. Intron-exon junction/splice site
B. Translational start site
C. Stop codon
D. Anywhere within the exonic sequences
A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction
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