Explain the swab method, Biology

Assignment Help:

Explain the Swab Method?

Swab method is the oldest and widely used method in food and dairy industry and was developed by W.A. Manheimer and T. Ybanez in 1917. A sterile cotton swab is used which is made up of wound cotton head on a 12-15 cm long wooden stick. It is moistened with a sterile rinse solution and used for rubbing the surface to be examined.

Swabbing is the most commonly used method to sample food contact surfaces. It is generally used for surfaces having high contaminant counts. Swab samples can be taken from any surface of the food processing facility like chillers, coolers, freezers, utensils, holding tanks, packaging machines, meat slicers, floor, walls, drains, working table, interior of a pipe or equipment piece etc. and analyzed by plating technique for total plate count. The exposed swab is kept back in the test tube containing a suitable diluent and kept in a refrigerator till used for plating. The organisms in the diluent are counted by SPC or any other method used for enumeration, as discussed earlier. Calcium alginate swabs can also be used in place of cotton swabs. Sterile 0.85% saline can be used to rinse the swab. It is used to hold microbial cell temporarily in stasis so that no change in number occur between the sampling and plating events.


Related Discussions:- Explain the swab method

Bomb calorimeter, Bomb calorimeter is used to determine the calorific value...

Bomb calorimeter is used to determine the calorific value of solid and liquid fuels. Construction          It consists of following parts 1.      Stainless steel bomb:

Can enhancers work in trans, Can enhancers work in trans? That is, can an e...

Can enhancers work in trans? That is, can an enhancer on one piece of DNA activate a promoter on another piece of DNA. Propose an experiment to test this. Be sure to discuss any ap

Explain about lymphatic system, Why in inflammatory and infectious conditio...

Why in inflammatory and infectious conditions may clinical signs related to the lymphatic system occur? The lymph nodes, or lymph glands, have lymphoid tissue that makes lympho

Depleted (vulnerable) species, Depleted (Vulnerable) Species These are...

Depleted (Vulnerable) Species These are the species whose numbers are greatly reduced from those of the recent past, and they are continuing to decrease. It is the continued d

What are the parts of the female reproductive system, What are the organs t...

What are the organs that are part of the female reproductive system? The organs that comprise the female reproductive system are the ovaries, the Fallopian tubes (or uterine t

A fruit fly has four pairs of chromosomes in its cells, A fruit fly has fou...

A fruit fly has four pairs of chromosomes in its cells. At meiosis, how many dissimilar combinations of maternal and paternal chromosomes are possible in the gametes? In gamet

Determine the function of osseointegration, Determine the function of Osseo...

Determine the function of Osseointegration Osseointegration is also a measure of implant stability, which occurs at two stages- primary and secondary. Primary stability has be

Consequences of shifting the chemical equilibrium, Q. What are the conseque...

Q. What are the consequences of shifting the chemical equilibrium of the formation of bicarbonate from carbon dioxide and water towards the increase of product (bicarbonate) format

How to produce a similar sickling effect, Which of the following amino acid...

Which of the following amino acids would you expect to produce a similar sickling effect if placed at position 6? Select all that apply. 1. Arginine 2. Leucine 3. Lysine

Uses of microbes in industry-citric acid, Normal 0 false fals...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd