Explain the swab method, Biology

Assignment Help:

Explain the Swab Method?

Swab method is the oldest and widely used method in food and dairy industry and was developed by W.A. Manheimer and T. Ybanez in 1917. A sterile cotton swab is used which is made up of wound cotton head on a 12-15 cm long wooden stick. It is moistened with a sterile rinse solution and used for rubbing the surface to be examined.

Swabbing is the most commonly used method to sample food contact surfaces. It is generally used for surfaces having high contaminant counts. Swab samples can be taken from any surface of the food processing facility like chillers, coolers, freezers, utensils, holding tanks, packaging machines, meat slicers, floor, walls, drains, working table, interior of a pipe or equipment piece etc. and analyzed by plating technique for total plate count. The exposed swab is kept back in the test tube containing a suitable diluent and kept in a refrigerator till used for plating. The organisms in the diluent are counted by SPC or any other method used for enumeration, as discussed earlier. Calcium alginate swabs can also be used in place of cotton swabs. Sterile 0.85% saline can be used to rinse the swab. It is used to hold microbial cell temporarily in stasis so that no change in number occur between the sampling and plating events.


Related Discussions:- Explain the swab method

Explain the urinary excretion of ascorbic acid, Explain the Urinary excreti...

Explain the Urinary excretion of Ascorbic Acid? The urinary excretion of ascorbic acid falls when the dietary intake is low. Since the tissues are not saturated, a greater part

Winter stratification - thermal stratification, Winter Stratification - The...

Winter Stratification - Thermal stratification During extremes of winter the surface layer of the lake freezes or attains a temperature close to 0° Celsius. Under these condit

What is chylomicrons , Chylomicrons, the widest of the lipoproteins, are...

Chylomicrons, the widest of the lipoproteins, are synthesized in the intestine. They including ingested triacylglycerols to other tissues, mainly skeletal muscle and adipose tissue

What is the goal of biological classification, The goals of biological clas...

The goals of biological classification The world of animal diversity is quite complex and it requires an ability to recognise similarities and differences among organisms. Class

What is polymerase chain reactionin genetics, What is Polymerase Chain Reac...

What is Polymerase Chain Reactionin genetics? A great advance in sequencing and cloning DNA is the polymerase chain reaction, devised by the American biochemist Kary Mullis in

Complications during angina pectoris, Q. Complications during angina pector...

Q. Complications during angina pectoris? It is a symptom giving a warning of impending myocardial infarction, sudden cardiac death or even ischemic necrosis of the brain leadin

The progeny is expected to have homozygous genotypes, If a plant of genotyp...

If a plant of genotype A/a is selfed, and numerous offsprings are scored, what proportion of the progeny is expected to have homozygous genotypes?

Biochemical methods of detecting tumors, Based on your knowledge of neoplas...

Based on your knowledge of neoplasia, discuss biochemical methods of detecting tumors, and imaging characteristics (in your particular imaging modality) that suggest malignancy. Ex

What is the mechanism of elisa, ELIZA stands for enzyme linked immune diffu...

ELIZA stands for enzyme linked immune diffusion assays. It works on standard of antigen antibody reaction.

Acute myocardial infarction with haemodynamic deterioration, Acute Myocardi...

Acute Myocardial Infarction with Haemodynamic Deterioration :  These patients may require urgent insertion of intra aortic balloon pump (IABP) followed by PTCA or surgery. 50 per

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd