Explain the swab method, Biology

Assignment Help:

Explain the Swab Method?

Swab method is the oldest and widely used method in food and dairy industry and was developed by W.A. Manheimer and T. Ybanez in 1917. A sterile cotton swab is used which is made up of wound cotton head on a 12-15 cm long wooden stick. It is moistened with a sterile rinse solution and used for rubbing the surface to be examined.

Swabbing is the most commonly used method to sample food contact surfaces. It is generally used for surfaces having high contaminant counts. Swab samples can be taken from any surface of the food processing facility like chillers, coolers, freezers, utensils, holding tanks, packaging machines, meat slicers, floor, walls, drains, working table, interior of a pipe or equipment piece etc. and analyzed by plating technique for total plate count. The exposed swab is kept back in the test tube containing a suitable diluent and kept in a refrigerator till used for plating. The organisms in the diluent are counted by SPC or any other method used for enumeration, as discussed earlier. Calcium alginate swabs can also be used in place of cotton swabs. Sterile 0.85% saline can be used to rinse the swab. It is used to hold microbial cell temporarily in stasis so that no change in number occur between the sampling and plating events.


Related Discussions:- Explain the swab method

Air can enter a plant through the leaf, Air can enter a plant through the l...

Air can enter a plant through the leaf Procure a leaf with a long stalk to it and seal it into a hole through a cork. Fit this with a side tube, and seal the cork into a flask

Describe the ways in which a genome can be sequenced, 1. Describe what effe...

1. Describe what effects epigenetic modifications such as methylation and acetylation can have in eukaryotic cells, and how these modifications are added or removed from chromatin.

Explain the coliforms - microbiological study of water, Explain the Colifor...

Explain the Coliforms - Microbiological Study of Water? These are widely used indicators which belongs to the family Enterobacteriaceae and make up about 10% of the intestinal

Define utilization of soy protein concentrate, Define Utilization of Soy Pr...

Define Utilization of Soy Protein Concentrate? Soy protein concentrates are used in food products for their nutritional characteristics or for their functional properties or fo

List the four objectives of counselling a diabetic patient, Q. List the fou...

Q. List the four objectives of counselling a diabetic patient? Objectives of Diabetes Counselling 1. Facilitating decision to start treatment and change life style 2.

What is physical activity ratio, What is Physical activity ratio? Physi...

What is Physical activity ratio? Physical activity ratio (PAR): The energy cost of an activity per unit of time (usually a minute or an hour) expressed as a multiple of BMR. It

Define techniques for detection hidden root canals, Define Techniques for D...

Define Techniques for Detection Hidden Root Canals Three well-angulated periapical films for 3D morphology: o With the cone directed straight on. o Mesiobilque. o And disto

Explain the history of darwins finches, Explain the history of Darwin's Fin...

Explain the history of Darwin's Finches? The most well known evidence upon which Darwin based his theory was the distribution pattern of the Galapagos finches. The Beagle's voy

Assessment of development in children, Assessment of Development in Childre...

Assessment of Development in Children The development of a child is studied through various responses which he exhibits following a natural or experimental stimulus. Basical

What is the function of mineral salts, Q. What is the function of mineral s...

Q. What is the function of mineral salts in the creation of electric tension (voltage) at the cellular level? The electric activity of the cell, for example, in neurons, depend

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd