Explain the swab method, Biology

Assignment Help:

Explain the Swab Method?

Swab method is the oldest and widely used method in food and dairy industry and was developed by W.A. Manheimer and T. Ybanez in 1917. A sterile cotton swab is used which is made up of wound cotton head on a 12-15 cm long wooden stick. It is moistened with a sterile rinse solution and used for rubbing the surface to be examined.

Swabbing is the most commonly used method to sample food contact surfaces. It is generally used for surfaces having high contaminant counts. Swab samples can be taken from any surface of the food processing facility like chillers, coolers, freezers, utensils, holding tanks, packaging machines, meat slicers, floor, walls, drains, working table, interior of a pipe or equipment piece etc. and analyzed by plating technique for total plate count. The exposed swab is kept back in the test tube containing a suitable diluent and kept in a refrigerator till used for plating. The organisms in the diluent are counted by SPC or any other method used for enumeration, as discussed earlier. Calcium alginate swabs can also be used in place of cotton swabs. Sterile 0.85% saline can be used to rinse the swab. It is used to hold microbial cell temporarily in stasis so that no change in number occur between the sampling and plating events.


Related Discussions:- Explain the swab method

Is the ventricle lumen larger during diastole, Q. Is the ventricle lumen la...

Q. Is the ventricle lumen larger during diastole or during systole? During diastole the opposite occurs. The muscle fibers of the ventricles relax and the lumen of these chambe

Explain the determination of nicotinic acid, Determination of Nicotinic aci...

Determination of Nicotinic acid The chemical methods of assay are based on colour reactions of pyridine. Nicotinic acid and nicotinamide are converted by cyanogen bromide into

Paratyphoid and other salmonella infections, P a r a typhoid and other ...

P a r a typhoid and other Salmonella infections Paratyphoid salmonellae are non-host-specific. The commonly reported species are S. Typhimurium, S . Enteritidis S . T

Substances that facilitate to compose the plasma membrane, Q. What are the ...

Q. What are the chemical substances that facilitate to compose the plasma membrane? The major constituents of the plasma membrane are phospholipids, proteins and carbohydrates.

Rheumatic fever, Rheumatic fever is an immunologically mediated connective ...

Rheumatic fever is an immunologically mediated connective tissue disorder following throat infection with group-A streptococci (GAS). It is characterised by an inflammatory proces

Describe about defecation reflex, Q. Describe about defecation reflex? ...

Q. Describe about defecation reflex? An example of a reflex having an autonomic (and somatic) component is the DEFECATION REFLEX: The rectum is generally empty, but when fec

Coarctation of aorta, Careful palpation of the upper and lower limb pulses ...

Careful palpation of the upper and lower limb pulses would make one suspect coarctation as the cause of hypertension. The lower limb pulses are weak and delayed. Confirmation ca

Production efficiency - ecosystem, Production Efficiency - Ecosystem T...

Production Efficiency - Ecosystem The maximum amount of solar energy harvested by plants is about 5 per cent but the average for green plants, on the whole is only a small fra

Depth and currents, Depth and Currents Depth The sea is v...

Depth and Currents Depth The sea is very deep varying in different regions. Generally life extends to all depths but is confined more to the continental shelf and

Botany, Sir/Madam, I am a Banquet Farm Owner and face the problem with the ...

Sir/Madam, I am a Banquet Farm Owner and face the problem with the Grass that in November & December the grass does not grow well or disappear beacuse of heavy foot fall. Is their

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd