Explain the swab method, Biology

Assignment Help:

Explain the Swab Method?

Swab method is the oldest and widely used method in food and dairy industry and was developed by W.A. Manheimer and T. Ybanez in 1917. A sterile cotton swab is used which is made up of wound cotton head on a 12-15 cm long wooden stick. It is moistened with a sterile rinse solution and used for rubbing the surface to be examined.

Swabbing is the most commonly used method to sample food contact surfaces. It is generally used for surfaces having high contaminant counts. Swab samples can be taken from any surface of the food processing facility like chillers, coolers, freezers, utensils, holding tanks, packaging machines, meat slicers, floor, walls, drains, working table, interior of a pipe or equipment piece etc. and analyzed by plating technique for total plate count. The exposed swab is kept back in the test tube containing a suitable diluent and kept in a refrigerator till used for plating. The organisms in the diluent are counted by SPC or any other method used for enumeration, as discussed earlier. Calcium alginate swabs can also be used in place of cotton swabs. Sterile 0.85% saline can be used to rinse the swab. It is used to hold microbial cell temporarily in stasis so that no change in number occur between the sampling and plating events.


Related Discussions:- Explain the swab method

What do you mean by herbaria and museums, Q. What do you mean by Herbaria a...

Q. What do you mean by Herbaria and Museums? A herbarium is a collection of pressed and dried plants arranged according to some valid system of classification and available for

Show gastrointestinal diseases and disorders, Q. Show Gastrointestinal Dise...

Q. Show Gastrointestinal Diseases and Disorders? Before discussing the many gastrointestinal problems, it is useful to understand the basic physiology of the gastrointestinal t

Mosaic endosperm - variants of endosperm, Mosaic Endosperm - Variants of En...

Mosaic Endosperm - Variants of Endosperm In some plants patches of two different colors appear in the tissues of the endosperm providing a mosaic design. In maize, red and whi

Define the carbohydrate utilization pattern test, Define the Carbohydrate U...

Define the Carbohydrate Utilization Pattern Test? Carbohydrates are composed of carbon, hydrogen and oxygen. These organic molecules are catabolized by large number of microorg

Determine the cardio-vascular system, Determine the Cardio-vascular System ...

Determine the Cardio-vascular System Cardio-vascular system consists of a heart and blood vessels. The function of cardio- vascular system is to maintain blood supply to all t

Middle ear, MIDDL E EAR - Protected by tympanic bone. It's cavity...

MIDDL E EAR - Protected by tympanic bone. It's cavity is tympanic cavity. It is lined by simple cilliated columnar epithelium. It is connected to naso pharynx by Eu

How does the contraceptive diaphragm work, Q. How does the contraceptive di...

Q. How does the contraceptive diaphragm work? What are the limitations of this contraceptive method? The contraceptive diaphragm is an artifact made of plastic or latex that wh

Poverty and malnutrition, Poverty and Malnutrition Poverty and malnutr...

Poverty and Malnutrition Poverty and malnutrition are closely related. As noted before, many elementary aspects of being poor like hunger, inadequate health care, unhygienic l

Reproduction, what is the difference between anisogamy and oogamy?

what is the difference between anisogamy and oogamy?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd