Explain the swab method, Biology

Assignment Help:

Explain the Swab Method?

Swab method is the oldest and widely used method in food and dairy industry and was developed by W.A. Manheimer and T. Ybanez in 1917. A sterile cotton swab is used which is made up of wound cotton head on a 12-15 cm long wooden stick. It is moistened with a sterile rinse solution and used for rubbing the surface to be examined.

Swabbing is the most commonly used method to sample food contact surfaces. It is generally used for surfaces having high contaminant counts. Swab samples can be taken from any surface of the food processing facility like chillers, coolers, freezers, utensils, holding tanks, packaging machines, meat slicers, floor, walls, drains, working table, interior of a pipe or equipment piece etc. and analyzed by plating technique for total plate count. The exposed swab is kept back in the test tube containing a suitable diluent and kept in a refrigerator till used for plating. The organisms in the diluent are counted by SPC or any other method used for enumeration, as discussed earlier. Calcium alginate swabs can also be used in place of cotton swabs. Sterile 0.85% saline can be used to rinse the swab. It is used to hold microbial cell temporarily in stasis so that no change in number occur between the sampling and plating events.


Related Discussions:- Explain the swab method

How does one get infected by etec, Q. How does one get infected by Enteroto...

Q. How does one get infected by Enterotoxigenic escherichia coli gastroenteritis(ETEC)? Infection occurs when a person eats food or drinks water or ice contaminated with ETEC

Differance between rescue-capacity-building strategy, QUESTIONS: Write ...

QUESTIONS: Write an analysis of the Chapter Twelve, the concluding chapter in ROLL AWAY THE STONE. It will be worth UP TO 50 points if you do it well. Submit it through the "Ex

Male reproductive disorders-cryptorchidism, Cryptorchidism Cryptorchid...

Cryptorchidism Cryptorchidism or incomplete descent of testis or retention of testis in the abdominal cavity occurs in all domestic species but is seen most commonly in stalli

How many cellular nuclei does the pollen tube have, How many cellular nucle...

How many cellular nuclei does the pollen tube of angiosperms have? What is the ploidy of each of these nuclei? The pollen tube that is the mature male gametophyte of angiosperm

Speculate why stoma in the lower epidermis, Carbon dioxide needed for photo...

Carbon dioxide needed for photosynthesis enters the leaf via the stomata but water is also lost when these pores are open. Water is often limiting and to limit water loss through s

Porcine rotavirus, Porcine rotavirus Piglet diarrhoea and mortality are ga...

Porcine rotavirus Piglet diarrhoea and mortality are gaining importance and the viral agent which is highly incriminated in such episodes is porcine rotavirus. The clinical featur

Explain skeletal muscle, Explain Skeletal muscle Skeletal muscle has...

Explain Skeletal muscle Skeletal muscle has low activity of glucose-6-phosphate dehydogenase. Yet, like most other  tissues  it can synthesize ribose-5-phosphate.  This  is.

Describe genetic equilibrium is maintained in population, a) How does the H...

a) How does the Hardy- Weinberg's expression (p²+2pq+q² =1) Describe that genetic equilibrium is maintained in a population? (b)  List any two factors that can disturb the ge

What is the computed tomograpy scan, Computed Tomograpy Scan Developmen...

Computed Tomograpy Scan Developments in cross-sectional imaging techniques such as spiral tomography and reformatted computerized tomograms have become increasingly popular in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd