Explain the swab method, Biology

Assignment Help:

Explain the Swab Method?

Swab method is the oldest and widely used method in food and dairy industry and was developed by W.A. Manheimer and T. Ybanez in 1917. A sterile cotton swab is used which is made up of wound cotton head on a 12-15 cm long wooden stick. It is moistened with a sterile rinse solution and used for rubbing the surface to be examined.

Swabbing is the most commonly used method to sample food contact surfaces. It is generally used for surfaces having high contaminant counts. Swab samples can be taken from any surface of the food processing facility like chillers, coolers, freezers, utensils, holding tanks, packaging machines, meat slicers, floor, walls, drains, working table, interior of a pipe or equipment piece etc. and analyzed by plating technique for total plate count. The exposed swab is kept back in the test tube containing a suitable diluent and kept in a refrigerator till used for plating. The organisms in the diluent are counted by SPC or any other method used for enumeration, as discussed earlier. Calcium alginate swabs can also be used in place of cotton swabs. Sterile 0.85% saline can be used to rinse the swab. It is used to hold microbial cell temporarily in stasis so that no change in number occur between the sampling and plating events.


Related Discussions:- Explain the swab method

How vitamin e is directly related to vitamins a, How vitamin e is directly ...

How vitamin e is directly related to Vitamins A, D and K? A high intake of vitamin E interferes with the functions of other fat-soluble vitamins such as vitamin K absorpt

Reflexes, Reflexes It is used to assess the neurobehavioural status....

Reflexes It is used to assess the neurobehavioural status. Rooting reflex - Being a primitive reflex, if cheek is rubbed, the infant will turn his head into that dire

Classification of microorganisms basis of temperature, Classification of Mi...

Classification of Microorganisms On basis of temperature for growth  Microorganisms can be classified into: -  Thermophillic: Microbes those require high temperature for

Briefly explain about gelation, Explain Gelation Gelation, which occurs...

Explain Gelation Gelation, which occurs when yolk is frozen and thawed, is apparently due to the aggregation of yolk lipoproteins because  of the imbalance and shift in water.

What are the major harms caused by vitamin a deficiency, Q. What are the ma...

Q. What are the major harms caused by vitamin A deficiency? How does this vitamin act in the physiology of vision? Deficiency of vitamin A (retinol) may corneal dryness (xeroph

Difference between hazard and disaster, 1.      Hazard Is An Extreme Event ...

1.      Hazard Is An Extreme Event Whereas Disaster Is Its Consequence. 2.      A hazard can occur without human interference but it may become a disaster on account of careless

Locomotion in leech, LOCOMOTION IN LEECH - Leech moves by looping actio...

LOCOMOTION IN LEECH - Leech moves by looping action of its body on substratum. Posterior sucker is attached to base, body elongates, then anterior sucker is attached. Now po

Biodiverse ecosystems of economic application, Q. What are some economic ap...

Q. What are some economic applications that can be generated by very biodiverse ecosystems? Extremely biodiverse areas present huge economic potential and they can be a source

The oxidative phase generates nadph, 1)  The oxidative phase  generates N...

1)  The oxidative phase  generates NADPH The oxidative branch of  the pathway  generates NADPH  and pentose-5-phosphate, through the following  reactions: a)  Glucose-6-pho

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd