Explain the swab method, Biology

Assignment Help:

Explain the Swab Method?

Swab method is the oldest and widely used method in food and dairy industry and was developed by W.A. Manheimer and T. Ybanez in 1917. A sterile cotton swab is used which is made up of wound cotton head on a 12-15 cm long wooden stick. It is moistened with a sterile rinse solution and used for rubbing the surface to be examined.

Swabbing is the most commonly used method to sample food contact surfaces. It is generally used for surfaces having high contaminant counts. Swab samples can be taken from any surface of the food processing facility like chillers, coolers, freezers, utensils, holding tanks, packaging machines, meat slicers, floor, walls, drains, working table, interior of a pipe or equipment piece etc. and analyzed by plating technique for total plate count. The exposed swab is kept back in the test tube containing a suitable diluent and kept in a refrigerator till used for plating. The organisms in the diluent are counted by SPC or any other method used for enumeration, as discussed earlier. Calcium alginate swabs can also be used in place of cotton swabs. Sterile 0.85% saline can be used to rinse the swab. It is used to hold microbial cell temporarily in stasis so that no change in number occur between the sampling and plating events.


Related Discussions:- Explain the swab method

Bacteriophage, Bacteriophage is a virus which infects a bacterium and whic...

Bacteriophage is a virus which infects a bacterium and which is many times used in molecular genetics experiments as the vector, or cloning vehicle. Recombinant phages can be made

#title., what organ stores glycogen

what organ stores glycogen

Determine the method of recombination, Which of the following is a false st...

Which of the following is a false statement regarding the method of recombination or crossing-over? A. Crossing-over takes place during prophase I of meiosis B. Recombinatio

How is heart contraction triggered, How is heart contraction triggered? ...

How is heart contraction triggered? Heart contraction is independent from neuronal stimulus (although it can be modulated by the autonomous nervous system). In the heart there

Discuss about human ears, Ears We have a pair of ears. Each ear has thr...

Ears We have a pair of ears. Each ear has three parts - external, middle and inner ear. The function of the middle ear is transmission of sound waves from external to inner ear

What is alkaline phosphatase, Question 1 Discuss the quantitative methods ...

Question 1 Discuss the quantitative methods for the estimation of urinary proteins. What are the instructions to be given to a patient while collecting urine for biochemical exami

Explain about the vascular tissues, Is transportation of gases in tracheoph...

Is transportation of gases in tracheophytes made through the vascular tissues? Carbon dioxide and oxygen are not transported by the xylem or phloem. These gases reach the cells

Define the use of computers in numerical taxonomy, Define the Use of Comput...

Define the Use of Computers in Numerical Taxonomy The use of computers in numerical taxonomy has made it possible to compare a large number of characters from many organisms wi

Develop questions which will facilitate effective research, Theories are im...

Theories are import because they can be used to a. Test research hypothesis b. Develop questions which will facilitate more effective research c. Conduct more meaningful and useful

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd