Explain the requirements of special stains, Biology

Assignment Help:

Explain the requirements of Special Stains?

Special stains are required to make spores clearly visible. Different techniques like negative staining, dorner staining method, schaeffer fulton staining method can be used. The most commonly used one is a schaeffer-fulton method. It is a differential staining technique using 2 different reagents - Malachite green and Safranin.

Malachite green is a primary stain. Spores do not take the stain easily, so heating is applied by steaming for about 5 minutes. This makes the stain penetrate the endospore wall and make vegetative cell, as well as, endospores stain green as you can see in the margin illustration. After primary stain, decolourization is done by tap water. Once the slide is cooled down, washing with tap water is done for about 30 seconds to one minute to remove the excess primary stain. As the stain does not have the strong affinity for vegetative cell, it is removed from the vegetative cell by tap water. So vegetative cells become colourless and spores remain green. Safranin is used as a counter stain. It is absorbed by the vegetative cells, which become colourless during decolourizing step. So after staining by this technique, spores will appear green and vegetative cells red.


Related Discussions:- Explain the requirements of special stains

How many different types of pseudopods can amoebas have?, Tests of shelled ...

Tests of shelled amoebas are made from a variety of substances including sand grains, chitin and substrate, calcium salts, and even silica. These shells still accumulate in ocean d

Describe the method of molecular photo copying, Question 1: "Plant tiss...

Question 1: "Plant tissue culture now has direct commercial applications" Explain. Describe plant tissue culture Illustrate importance of plant tissue culture Sho

Parthenogenesis, Parthenogenesis The diploid egg produced in the embr...

Parthenogenesis The diploid egg produced in the embryo sacs during diplospory and apospory develops into an embryo without fertilization, thus maintaining the sporophytic lev

Abscisic acid, Abscisic Acid Abscisic acid (ABA) as a naturally occurr...

Abscisic Acid Abscisic acid (ABA) as a naturally occurring growth inhibitor was discovered through independent investigations of different physiological phenomena in two diffe

Define some precautions for estimation of amount of bacteria, Define some P...

Define some Precautions for Estimation of the Amount of Bacteria? 1. Dilution should be made carefully. 2. Use fresh sterile pipette for making each dilution. 3. Aseptic

Define water balance - hormonal control of fluid balance, Define Regulation...

Define Regulation of Water Balance - Hormonal Control of Fluid Balance? Hormonal control of fluid balance: When water intake is insufficient or water loss is excessive, the kid

Uses of plural embryos, Uses of Plural Embryos Although the basic tha...

Uses of Plural Embryos Although the basic that trigger causes polyembryony are not fully understood, there has been no dearth of interest in exploiting supernumerary embryos.

Define the process of digestion of proteins, Define the Process of Digestio...

Define the Process of Digestion of Proteins? The daily protein intake (of about 50-100 g) and the protein of enzymes, sloughed (bled or drop off) epithelial cells and mucins, w

Higher calorific value (hcv) or gross calorific value (gcv), It is defined ...

It is defined as the total amount of heat produced, when unit mass/volume of the fuel has been burnt completely and the products have been cooled to room temperature (~ 15 0 C).

Explain adefovir dipivoxil, Adefovir dipivoxil (Hepsera)  This phosphon...

Adefovir dipivoxil (Hepsera)  This phosphonate nucleoside analog, administered as the oral prodrug, inhibits replication of HBV, including variants resistant to lamivudine. Ade

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd