Explain the requirements of special stains, Biology

Assignment Help:

Explain the requirements of Special Stains?

Special stains are required to make spores clearly visible. Different techniques like negative staining, dorner staining method, schaeffer fulton staining method can be used. The most commonly used one is a schaeffer-fulton method. It is a differential staining technique using 2 different reagents - Malachite green and Safranin.

Malachite green is a primary stain. Spores do not take the stain easily, so heating is applied by steaming for about 5 minutes. This makes the stain penetrate the endospore wall and make vegetative cell, as well as, endospores stain green as you can see in the margin illustration. After primary stain, decolourization is done by tap water. Once the slide is cooled down, washing with tap water is done for about 30 seconds to one minute to remove the excess primary stain. As the stain does not have the strong affinity for vegetative cell, it is removed from the vegetative cell by tap water. So vegetative cells become colourless and spores remain green. Safranin is used as a counter stain. It is absorbed by the vegetative cells, which become colourless during decolourizing step. So after staining by this technique, spores will appear green and vegetative cells red.


Related Discussions:- Explain the requirements of special stains

Which groups does not contain a carbonyl, Which of the following functional...

Which of the following functional groups does NOT contain a carbonyl? Select one: a. Aldehyde b. Carboxyl c. Phosphate d. Amide e. Organic ester

Explain some physiological changes during pregnancy, Explain some Physiolog...

Explain some Physiological Changes during Pregnancy? A whole new life begins at conception. Organ systems develop rapidly and nutrition plays many supportive roles. Pregnancy f

Define objectives to know about methods of food processing, Define Objectiv...

Define Objectives to know about methods of food processing? After studying this unit, you will be able to: Enumerate the different methods of food processing Enlist

Health education to hospitalised child, Health Education to Hospitalised C...

Health Education to Hospitalised Child You have unique opportunity for teaching and reinforcing good hygienic care while the child is hospitalised. Clean hair, nails, clothin

Anatomic factors in patient selection for mitral valvuloplas, Q. Anatomic F...

Q. Anatomic Factors in Patient Selection for Mitral Valvuloplasty ? The ideal patient is young, has pliable non calcified mitral leaflets, and mild subvalvular disease. TEE may

How much ampicillin can dissolve in 400 ml, How much ampicillin (sodium sal...

How much ampicillin (sodium sal, mw=371.40) would you dissolve in 400 mL of water to make 80 mg/ml solution of ampicillin?

Streptococcal antibody test, In about 80 per cent of ARF patients, ASO titr...

In about 80 per cent of ARF patients, ASO titre is significantly raised. ASO titres vary with age, geographical area and other fevers, which influence frequency of streptococcal in

Explain meningococcal disease, Meningococcal disease  Meningococcal vac...

Meningococcal disease  Meningococcal vaccine is recommended for adults and children >2 years old who are traveling to areas where epidemics are occurring, or to the "meningitis

Write short note on gluconeogenesis, Gluconeogenesis that is also called as...

Gluconeogenesis that is also called as GNG is a metabolic pathway which results in the generation of glucose from non-carbohydrate carbon substrates such as pyruvate, glycerol, lac

Explain the infraorbital nerve and vessels, Infraorbital nerve and vessels ...

Infraorbital nerve and vessels The infraorbital nerve exits through the infraorbital foramen. This foramen and the neurovascular contents are within 5 to10mm of extremely resor

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd