Explain the process of solubility test, Biology

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Explain the Process of Solubility test?

This test is given by all monosaccharides and disaccharides. Polysaccharides are not soluble in cold water.

Principle

All monosaccharides are soluble in water and insoluble in non polar substances. Since glucose, fructose and galactose are monosaccharides they will be soluble in water. Disaccharides readily dissolve in water. Starch is insoluble in cold water. In hot water it makes a colloidal solution. Dextrin being a less complex molecule is more soluble than starch.

Test

 Reagents Required 

  Methodology

Observation

Solubility test

 

 - Glucose, fructose, galactose powder, lactose, maltose, sucrose, starch.

 - Distilled water (hot and cold)

Take a 10 ml test tube and put a little glucose powder in it. Add about 2 ml of distilled water to test the solubility of the sugar in cold water. Repeat this with fructose and galactose separately. Try dissolving the sugars in hot distilled water.

A clear solution of all the three sugars will be observed both with hot and cold water. All

Monosaccharides are soluble in water.

Disaccharides readily dissolve in water.

Starch is insoluble in cold water. In hot water it forms a colloidal solution.

Dextrin being a less complex molecule is more soluble than starch.

 

 

 


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