Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Explain the Procedure for Preparation of Bacterial Smear?
Start the exercise by following the steps enumerated herewith.
(1) Take properly washed and dried glass slides for making bacterial smear. If slides are oily or greasy, wash them properly with soap and water followed by water rinse and a rinse with 95% alcohol. The slides are then dried.
(2) Now, label the slides properly by writing organism's name on either end of the slide by glassware marking pencil or permanent marker.
(3) Prepare the bacterial smear by using either liquid or solid culture medium as discussed herewith. From Liquid Culture - Re-suspend the sedimented cells in broth culture by tapping the culture tube with finger. Spread one or two loopfuls of the culture with a sterile inoculating loop over the center of the slide. Allow smear to air dry. From Solid Culture - Place a loopful of water over the center of the slide. Transfer the culture to the drop with a sterile, cooled loop. Mix small amount of cells with this drop and spread in a circular motion of the loop. Allow smear to air dry completely. The smear should appear as semitransparent or translucent whitish film.
(4) Fix the bacterial smear on glass slide by heat fixation. This can be accomplished by rapid passage of air-dried smear over the Bunsen burner flame two-three times. So you have done this. The slide is ready for staining.
(5) Examine each slide for the confluent, whitish film or haze.
Define Serum Vitamin A Content? Assessment of serum vitamin A content is the most reliable criterion Tor assessing vitamin A status. Serum levels indicative of various degrees
High fibre diets High fibre diets: The patients are advised to eat high fibre cereals as whole grain flour and bread, whole grain bread cereals, whole wheat pasta and b
Are the phloem and the xylem made of living cells? The cells of phloem are living cells and the cells that constitute the xylem ducts are dead cells killed by the lignin deposi
Q What is the major external morphological feature that differentiates platyhelminthes from other worms (nematodes)? Platyhelminthes are also recognized as flatworms because th
Seminal plasma in human males is rich in : 1. fructose and calcium 2. glucose and calcium 3. DNA and testosterone 4. ribose and potassium Fructose and Calcium
How many of the same proteins are made at the same time by each ribosome in the translation of one mRNA molecule? How does consecutive protein production occur in translation?
Q. What is the difference between primary ecological succession and secondary ecological succession? The Primary ecological succession is the changing sequence of communities f
Determine some Common Micronutrient Deficiencies? Vitamin A deficiency Iron deficiency anaemia Iodine deficiency disorders Zinc deficiency
Vascular Cambium The vascular cambium usually, if not always, has a dual origin within the primary tissues; from provascular strands, and from the "ground" meristem tissues be
habitat, habit, cell wall chemistry, reason why cell wall like this of Archaebacteria , eubacteria,fungi,algae,bryophytes ,pteridophyes, gymnosperms , angiosperms..
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd