Explain the principle or theory of biuret method, Biology

Assignment Help:

Explain the Principle or Theory of biuret method?

If a strongly alkaline solution of Biuret is heated with very dilute copper sulphate, a violet colour is obtained. The substances which contain two carbamyl (-CONH2) groups joined together either directly or through a single carbon or nitrogen atom and those which contain two or more peptide links give a blue or purple coloured complex with alkaline copper solution.

 

23_Principle or Theory of biuret method.png

Proteins respond positively since there are pairs of -CONH2 groups in the molecule. The reaction is due to coordination of cupric anion with the unshared electron pair of peptide nitrogen and the oxygen of the water. A coordination complex is formed which is purplish coloured and can be colorimetrically measured. When protein solution is made strongly alkaline with NaOH or KOH and very dilute CuSO4 is added, a purplish to pink violet colour is obtained, the intensity of the colour depending upon the concentration of protein or the number of peptide bonds undergoing the reaction.


Related Discussions:- Explain the principle or theory of biuret method

Primary succession - community change, Primary Succession - Community Chang...

Primary Succession - Community Change In primary succession on a terrestrial site the new site is first colonised by a few hardy pioneer species that are often microbes, liche

Cause the inhibition of apoptosis, We now understand that mutations that ca...

We now understand that mutations that cause the inhibition of apoptosis are found in tumors. Because proliferation itself is not induced by the inhibition of apoptosis, explain how

What are the possible phenotypes of the progeny, A (w+/ w+) red-eyed Drosop...

A (w+/ w+) red-eyed Drosophila female is crossed with a white-eyed male. Assuming the trait for eye colour is sex-linked, what are the possible phenotypes of the progeny?

Explain procedure for simple staining of bacterial cultures, Explain Proced...

Explain Procedure for Simple Staining of Bacterial Cultures? Now carry out the exercise following the steps given herewith: (1) Prepare thin bacterial smear on clean glass s

Hiatus hernia, Hiatus Hernia: In  this type of hernia, cardiac end of ...

Hiatus Hernia: In  this type of hernia, cardiac end of  the stomach passes through an abnormally wide oesophageal opening in the diaphragm  (see Fig. 2.6). Clinical manifestat

Records and reports - nursing service administration, RECORDS AND REPORTS: ...

RECORDS AND REPORTS: The principles of administration are described as  'POSDCORB'.  The  'R' stands for recording and reporting. Recording and reporting is related to all oth

Major conventional symbols & signs used in genetic family, What are the maj...

What are the major conventional symbols and signs used in genetic family trees? In the genetic family trees the male sex is usually represented by a square and the female by a

Determinants of health status, Determinants of Health Status The linkage...

Determinants of Health Status The linkage between economic development and health was discussed in the previous unit. The present unit deals with an identification of the factor

What are the main cellular functions of potassium, Q. What are the main cel...

Q. What are the main cellular functions of potassium? Moreover being important for the osmotic regulation and for the acid-base equilibrium (pH) potassium is fundamental for th

Reproduction, The fusion of two individuals with each acting as a gamete is...

The fusion of two individuals with each acting as a gamete is known as

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd