Explain the principle of nelson-somogyi method, Biology

Assignment Help:

Explain the Principle of Nelson-Somogyi Method?

Glucose is estimated by Nelson-Somogyi method. We begin our study of this method by getting to know the principle involved in this estimation.

Principle

All sugars which contain a free aldehyde group undergo enolization when placed in hot alkaline solution. The enediol form of sugars is highly reactive and is easily oxidized by oxidizing agents in alkaline solution. This property is utilized in the quantitative determination of sugars. The sugar solution is heated with alkaline copper reagent in a Folin's tube. The solution is made to react with arsenomolybdate solution to produce a blue colour. The intensity of this colour is measured colorimetrically.


Related Discussions:- Explain the principle of nelson-somogyi method

The cell cycle, what happens to the liver cell during the cell cycle

what happens to the liver cell during the cell cycle

Female reproductive tract, Female Reproductive Tract The female repro...

Female Reproductive Tract The female reproductive tract involves the fallopian tubes, uterus and vagina. Look at diagram. You would notice that the ovaries lay one on each si

Explain the filtration - air sampling, Explain the Filtration - Air Samplin...

Explain the Filtration - Air Sampling? Filtration - Air stream may also be filtered through a micro-filter.  Microorganisms are released from the filter using a suitable diluen

Genetic, Ask quesIn Cross 1, pure-breeding colored, starchy kernel plants (...

Ask quesIn Cross 1, pure-breeding colored, starchy kernel plants (C1Wx/C1Wx) were crossed to plants pure-breeding for colorless, waxy kernels (c1 wx/c1 wx). The F1 of this cross we

Whta is the effect of processing on mineral content of foods, Effect of pro...

Effect of processing on mineral content of foods Minerals are comparatively stable under processing conditions such as heat, light, use of oxidizing agents and extremes in pH.

Transport in human, what is the name of the valve that separates the chambe...

what is the name of the valve that separates the chamber?Why is that valve necessary?

Mechanisms of hemorrhage contention, Q. What are the mechanisms of hemorrha...

Q. What are the mechanisms of hemorrhage contention called? The physiological mechanisms of hemorrhage contention one of them is blood clotting are generically named hemostatic

How natural colourants produced, Natural Colors Used In Foods Natural c...

Natural Colors Used In Foods Natural colourants produced for use in an analogous way to the coal-tar dyes are crude extracts of pigments, which are basically unstable. The appa

Fundamental structure of the hiv virus, Q What is the fundamental structure...

Q What is the fundamental structure of the HIV virus? What is the function of the glycoproteins of its envelope? HIV is an RNA virus in its core there are two strands of RNA an

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd