Explain the principle of microscopy, Biology

Assignment Help:

Explain the Principle of Microscopy?

When the light passes from one medium to another, refraction occurs, i.e., the ray is bent at the interface. The direction and the magnitude of bending are determined by the refractive indices of the two media forming the interface. The refractive index is a measure of how greatly a substance slows the velocity of light when light passes from air to glass or vice versa. When the light rays strike the lens, a convex lens will focus these rays at specific point called focal point. The distance between the center of the lens and the focal point is called the focal length. Convex lens act as a magnifier.

It provides a clear magnifying image at a much closer range. Lens strength is related to focal length. A lens with a short focal length has a more magnification power than a lens having a longer focal length. Magnification means enlargement. In compound microscope, it is carried out by two-lens system - objective lens and ocular lens. Objective lens produces the real image of the specimen, which is projected up into focal plane and then magnified by the ocular lens to produce the final image. Though magnification is important, it has limits. Unlimited enlargement by increasing magnifying power of the lenses is not possible because of the limitations of resolving power. Microscope should not only produce the enlarged image but also the clear image. Resolution or resolving power of the lens is its ability to separate two close points as separate entities.


Related Discussions:- Explain the principle of microscopy

Ornithine-urea cycle, Normal 0 false false false EN-IN ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Who was charles darwin, Who was Charles Darwin? Charles Darwin was an Eng...

Who was Charles Darwin? Charles Darwin was an English naturalist born in 1809 and considered the father of the theory of evolution. At the end of the year 1831, before turning 23

S-gene, S-Gene The S-gene has been suggested to be a super gene comple...

S-Gene The S-gene has been suggested to be a super gene complex with several linked genes. It is supposed to have at least six (may he more) closely-linked genes which determi

Explain autosomal recessive disease, The frequency of an autosomal recessiv...

The frequency of an autosomal recessive disease in a population is 1 in a million. An unaffected person who had a sibling affected with this disease marries a lady from the pop

Explain the explosion puffing, Explain the Explosion Puffing? Explosion...

Explain the Explosion Puffing? Explosion puffing has been recognized as one of the most significant developments in dehydration technology. In explosion puffing, partially dehy

Name the important inorganic molecular for living being, What are the most ...

What are the most significant inorganic molecular substances for living beings? The most significant inorganic substances for living beings are mineral salts, water, carbon dio

How is genetic engineering used to create bacteria, How is genetic engineer...

How is genetic engineering used to create bacteria capable of producing human insulin? In a production of human insulin by bacteria the human insulin gene is incorporated into

Ability of mammalian kidney, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd