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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
What are the processes involved in the preparation of plant tissue for free hand sectioning?
Which is the kind of gamete (for a given trait) produced by a dominant homozygous individual? What is genotypical proportion of these gametes? And what about the recessive homozygo
Explain Hazard identification Hazard identification is "the identification of biological, chemical and physical agents capable of causing adverse health effects and which m
Types of Ecosystem Basically ecosystems are of two types : terrestrial and aquatic. If you travel from plains to the mountains in the Himalayas, you notice significant changes
what are the economic importance of obelia?
Occurrence of Jaundice Excessive production of bilirubin - this occurs in some diseases due to destruction of large number of red blood cells in the liver. Obstruction to
Explain the Pour Plate Method Isolated colonies can also be obtained by pour plate method. The method involves mixing of small volume of microbial suspension with molten nutrie
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Plant Responses to Specific Stress Conditions By now you must have developed a feeling that plants are organised to function optimally under a set of environmental conditions.
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