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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Q. What are the epithelial tissues? What is their general function and how is that function associated to the features of the tissue? Epithelial tissues also called as epitheli
Characteristic features of Phylum Cnidaria All are aquatic animals. Radial or biradial symmetry around an oro-aboral axis, but no head. Diploblastic, with an epidermi
Explain about the Cellular senescence - Geriatric Nutrition? Aging brings about an irreversible state of growth arrest at the cellular level. Though the cells are viable and me
What is Cyanosis ? Cyanosis: Cyanosis may be peripheral or central. Peripheral cyanosis almost exclusively involves lips and extremities. Normal neonates may have bluish extrem
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What is tree girdling? What happens to a plant when that girdle is removed from the stem (below the branches)? Malpighi's girdling, or tree girdling, is the removal from a ste
Q. Can you explain Cholera? Cholera is one of the diseases which had caused epidemics all over the world. The organism which causes the disease is Vibrio cholerae. It is an
Bacteriophage is a virus which infects a bacterium and which is many times used in molecular genetics experiments as the vector, or cloning vehicle. Recombinant phages can be made
Starr-Edward (S-E) Silastic Ball Valve Prosthesis : This was introduced in 1961 by Albert Stm and has different models for mitral and aortic positions. It is a cage and b
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