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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
What is the effect of physical activity on health? The Surgeon General's report (1996) summarizes the effects of physical activity on health as follows. Overall Mortality: H
Which factors are altered when cells are moved from an in vivo to an in vitro environment, and how do cells respond to these changes?
Interactions between Volatile Substances and Proteins Flavour binding may involve adsorption at the surface of food or penetration to the food interiority by diffu
Q. Explain the process of Degradation of Proteins? Proteins are composed of amino acids combined by peptide linkages. The native proteins are resistant to attack by microo
Explain about the Net Protein Ratio (NPR)? This method was introduced by Bender and Doell (1957) and is a modification of the PER method. In this method, an allowance is made f
1.sarcomastigophora 2.sporozoa 3.cnidospora 4.ciliophora
Life S cience is originated from latin word scientia , which means knowledge . Biology is a branch deals with living beings. Biology word is originated from t
Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment..... Question: Suppose restriction
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List three ways that DNA technology could be used to improve the lives of humans. Answers could include: identifying human remains, determining a person's paternity, tracing h
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