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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
In the evolution of whales, there is evidence that whales and hippo form a monophyletic group. Show all the evidence that supports the close relationship between whales and hippos
Explain the role of calcium ions, troponin and F-Actin during contraction in striated muscles of humans?
Q. Definition of Osseointegration in microscopic biophysical? Osseointegration implies that at light microscopic and electron microscopic levels, the identifiable components of
what are the consequences of parthenogenesis in human life if humans reproduced through parthenogenesis?
Functions of Synergids Ultra structurally the structure and concentration of cell organelles reveals that synergids are metabolically active. The following Wee functions are a
What would happen to a cell if it was placed into a hypertonic solution? Into a hypotonic solution? What would happen to a cell if the cell itself was hypertonic to the solution?
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Explain the Main Benefits of Canned Foods? The main benefits of canned foods are: (a) They are safe and hygienic and have logically good nutritional value; (b) They are e
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