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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Examination is begun by placing the transducer in left parasternal region, usually in the third or fourth left intercostal space. From this position, a sector image of the heart
While studying evolution, a student comes across a cladogram that includes clades like amphibia, reptilia, aves, and mammalia. What must be the basal clade?
DETERMIN A TIO N OF THE AGE OF FOSSIL - Radioactive clock (Boltwood -1907) - Half life of uranium is 4.5 billion years. This means half of total uranium disintegrates
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Q. How different are the concepts of action potential, resting potential and excitation threshold concerning neurons? Action potential is the maximum positive voltage level ach
The cystic fibrosis allele occurs in European populations with q=0.02, what fraction of this population can be expected to have cystic fibrosis?
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Explain Prophylaxis of lyme disease No vaccine is currently available to prevent Lyme disease in humans. Avoidance of ticks and use of tick repellents such as DEET or picaridin
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