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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
You have discovered that a single protein in two different cell lines is significantly over expressed in one of them. The proteins are identical, yet Northern blot analysis has dem
How the Rhizopus reproduce? Rhizopus usually reproduce asexually, but under unfavourable conditions, sexual reproduction (isogamous) occur resulting in formation of zygospores
Classification
Define Basic Terminology in Relation to Nutritional Requirements? By now you would have a good understanding that nutrient requirements are affected by a number of factors and
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Q. Which structures of the body have more bones for their size than any other part of the body? Hand and wrist have more bones in them for their size than any other part of bod
Gills - Respiratory Organs Gills are the specialised respiratory organs of several aquatic animals. They are found in mollusis and as well in many crustaceans. Typically gills
Explain Principle of Fehling's Soxhlet method (Lane-Eynon method)? Reducing sugars are those which have free sugar groups and hence may be estimated directly by titrating the s
MA L THU S THEORY OF HUMAN POPULATION GROWTH - Malthus put forward in 1778. 1. Population grows in geometrical ratio, where as the means grow in arithmetical ratio.
Few sentences about embryo
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