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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
give an example invertebrate phylum/phyla for protoplasmic grade organization
A scientist wanted to study the effect of a drug on the blood pressure of rats. She set up an experiment in which the experimental group consisted of rats that were injected with a
Homeostasis Homeostasis may be defined as the maintenance of constancy in the internal environment of the organism. This is essential for maintenance of life. Without homeost
critera for the animal classification
Define Procedure for estimation of vitamin c - colorimetric method? Carry out the experiment following the instructions given herewith: 1. Prepare a series of tubes for blan
Somatic Embryogenesis The process of embryo development is called embryogenesis. It is not the monopoly of the egg to form an embryo. Any cell of the female gametophyte (Embr
Organ Systems : Organisms are composed of organ systems. Each organ system is made up of several different organs. For example, the digestive system is composed of several organ
Q. What is Deoxynivalenol? Deoxynivalenol (DON) is a far more common, but much less toxic, trichothecene and is produced by species such as F. graminearum and F. culmorum. LD
Define Modification of Carbohydrate Intake for Specific Disorder? In our daily diet almost 60-7096' of energy is contributed by carbohydrates. Majority of them are comprised of
Strawberry Slain: It signfies the dilatation of deeper vessels to form cavernous spaces like those seen in the erectile tissue of the penis. The lesion is well-defined
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