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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Microtubule assembly: A) generally originates in the centrosome. B) Occurs only during mitosis. C) Occurs randomly throughout the cell. D) Is regulated by myosin. E) Is inhibited d
Hemerythrins - Respiratory Pigments The Hemerythrins are rather rare. They take place in some animals belonging to the minor phyla like the sipunculid worms, some brachiopods,
The main constituents of food are carbohydrates, lipids, and proteins but they are found in complex forms that cannot be directly utilized by the body. Hence, they are broken down
Define the Obesity in Children? It is difficult to measure overweight or obesity in children and adolescents because they grow and mature at different rates. Weight status in c
Q. What are the substances that are constitute the chromatin? What is the difference between chromosome and chromatin? The chromatin, dispersed in the nucleus, is a set of fila
PHYLUM ANNELIDA Definition and Introduction Bilateral and protosomial eucoelomate eumetazoans whose long narrow and wormlike body is division into ring like t
Explain about bimodal diurnal pattern Animals that show a bimodal diurnal pattern have their activity controlled by two biological clocks - one that controls the time of onset
Define Listeriosis monocytogenes L. monocytogenes can grow over the temperature range of about 1 0 to 45 0 C and the pH range 4.1 to around 9.6, it maybe expected to survive
What is the energy efficiency of photosynthesis? a) What area is occupied by each molecule? b) Calculate the molecular absorption cross section in units of A 2 A cr
Erwin Chargaff conducted a series of experiments in which he was calculating the amounts of dissimilar nucleotides that were present in dissimilar tissues and organisms. These stud
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