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Explain the Primary Stain - Ziehl-Neelsen Method?
Ordinary aqueous stains like methylene blue, crystal violet, etc. are not able to stain most of the species of Mycobacteria. Carbol fuchsin, a red mixture of basic fuchsin and phenol can be used for staining these bacteria. Basic fuchsin, binds to carboxylic acid group of mycolic acid and phenol enhances penetration of basic fuchsin into lipid. Penetration is further enhanced by slow heating to steaming for 2-3 minutes. This drives the stain inside the cytoplasm. In modified Ziehl-Neelsen method, instead of heating, a wetting agent - Turgitol - can be added to the stain. This agent reduces surface tension between the cell wall of the mycobacteria and the stain. After staining, all cells become red.
Classification of the Phylum Protozoa up to orders
The field of dietetics can be related to: Nutrition care and intervention focused on the individual, and Nutrition care and intervention focused on the group.
Of which type of defense cell do viral infections stimulate the multiplication? The major leukocytes that generally multiply and participate in the defense against viral infect
Explain Advantages and Disadvantages of Spread Plate Method? The advantages and disadvantages of spread plate method are highlighted next. Advantages 1. It is useful fo
Drug Intolerance For patients who cannot tolerate rifampin, alternative regimens include 9-12 months of isoniazid, ethambutol and pyrazinamide, with or without a fluoroquinolo
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Explain Surface properties of proteins The surface properties relates primarily to surface tension, a) Emulsification and b) Foaming characteristics of proteins,
Explain Micafungin sodium Micafungin sodium (Mycamine - Astellas), the second echinocandin antifungal1 to become available in the US, has been approved by the FDA for intraveno
Define the term - microfilaments and microtubules The cytoskeleton is, in fact, termed as the "bone and muscle" of the eukaryotic cell, and is composed of a network, consisting
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