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Explain the Presumptive Test - Most Probable Number Test?
The Presumptive test is used to detect and estimate coliform population of a water sample. This test is carried out by inoculating a series of lactose broth medium with three different sample volumes (10 ml, 1 ml and 0.1 ml). The series consists of 3 groups, each composed of 5 tubes and each group is inoculated with the designated volume of the water sample. The greater the number of tubes per group, the greater the sensitivity of the test. Lactose medium contains the lactose, bile salt and pH indicator.
Lactose and bile salts are selective for coliforms as lactose is used only by coliforms and not by other enteric bacteria while bile salts are depressent for enteric bacteria other than coliforms. A pH indicator such as bromocresol purple is added to the medium to detect acid fermentation from lactose. Production of acid changes the colour of the medium to yellow. Gas production during fermentation is detected by inverted Durham tube in lactose medium. Positive test for coliforms is indicated by the production of acid and gas from lactose fermentation after 24-48 hours of incubation. Number of tubes showing positive test is counted and the result is expressed as most probable number (MPN) of coliforms by matching the results
Producers Autotrophs (self-nourishing) are green plants as they synthesise carbohydrates from simple inorganic raw materials like carbon dioxide and water in the presence of su
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Will the plant-incorporated protectants rules affect the Bt reassessment? No. To make sure that our biotechnology assessments reflect the latest data on health and ecological e
In developing countries because of poor compliance, poverty, ignorance, primary and secondary prevention strategies are difficult to implement properly. Also in western countries (
Q. Why is rubella during gestation a threat to the fetus? If taking place during gestation rubella is a dangerous disease because the virus crosses the contaminates fetus and t
what is nutrition
Extraradicular Infections Whatever the cause of post endodontic disease we should do proper diagnosis to determine what is the cause; is it intra or extraradicular infection ,
Primary Prevention: Primary prevention should have the following goals: i) Ascertaining at the risk population and the high risk situations where stressful life events a
Explain about the Energy Requirements in elderly? Based on the Benedict - Harris equation for men and women, the daily energy requirements can be calculated. For Men: 66 t
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