Explain the precautions for sub-culturing of a culture, Biology

Assignment Help:

Explain the Precautions for Sub-Culturing of a Culture

1. Sterilization of inoculating wire before and after the transfer is a must. Sterilized wire should not be kept on laboratory bench.

2. Never place the closure of tubes or lid of the plates on working bench or touch it with any surface.

3. Neck of the tubes should be flamed before and after the inoculation to avoid contamination.

4. Culture transfer should be done near the flame.

5. Do not dip the needle into the agar while inoculating slant or agar plate.

6. Never leave the culture tube open any longer than the amount of time needed to transfer the culture.


Related Discussions:- Explain the precautions for sub-culturing of a culture

Tolerance range, Maximum and minimum range of an animal with the factors of...

Maximum and minimum range of an animal with the factors of Ph Salinity Pressure Relative human

What is pulmonary stenosis, Pulmonary Stenosis :  The obstruction may be v...

Pulmonary Stenosis :  The obstruction may be valvar, infundibular or supra valvar.

Explain the term phytates, Explain the term Phytates? We are familiar w...

Explain the term Phytates? We are familiar with phytates as an inhibitor of mineral absorption (calcium, iron etc.) especially in the vegetarian diets that are cereal-based. Th

Vertebrates - osmotic and ionic regulation, Normal 0 false fa...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What are the symptoms of dyslipidemia, Q. What are the Symptoms of dyslipid...

Q. What are the Symptoms of dyslipidemia? The main symptom is presence of xanthoma: This is a yellowish swelling, nodule or plaque in the skin resulting from deposits of fat. T

Define national iodine deficiency disorder control programme, Define Nation...

Define National Iodine Deficiency Disorder Control Programme (NIDDCP)? The prevalence of iodine deficiency disorders (IDD) are seen more among adolescent's young adults and sc

What is the molecular formula of the compound, A solid compound was found t...

A solid compound was found to contain 42.11% C, 51.46%O and 6.43% H by weight. The molecular weight of the compound was 341. What is the molecular formula of the compound?

Define the importance of iodine in human, Define the importance of Iodine i...

Define the importance of Iodine in human? Iodine derives the nutritional importance as a constituent of thyroid hormones, 3,5,3',5' tetraiodo-thyronine (thyroxine or T 4 ) and

Basic domains of dna, DNA binding domains called as basic domains rich in b...

DNA binding domains called as basic domains rich in basic amino acids that was happen in transcription factors in combination with zipper helix-loop-helix or leucine (HLH) dimeriza

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd